SitesBLAST
Comparing WP_086510332.1 NCBI__GCF_002151265.1:WP_086510332.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
57% identity, 99% coverage: 3:241/242 of query aligns to 1:239/240 of 4ymuJ
- binding adenosine-5'-triphosphate: F11 (= F13), V16 (= V18), S36 (= S38), G37 (= G39), S38 (= S40), G39 (= G41), K40 (= K42), S41 (= S43), T42 (= T44), E162 (= E164), H194 (= H196)
- binding magnesium ion: S41 (= S43), E162 (= E164)
3c4jA Abc protein artp in complex with atp-gamma-s
56% identity, 100% coverage: 1:241/242 of query aligns to 1:241/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
56% identity, 100% coverage: 1:241/242 of query aligns to 1:241/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
56% identity, 100% coverage: 1:241/242 of query aligns to 1:241/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
56% identity, 100% coverage: 1:241/242 of query aligns to 1:241/242 of 2oljA
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
59% identity, 99% coverage: 3:241/242 of query aligns to 2:239/241 of 4u00A
P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
50% identity, 96% coverage: 8:239/242 of query aligns to 11:254/258 of P02915
- S41 (= S38) binding ATP
- G42 (= G39) binding ATP
- G44 (= G41) binding ATP
- K45 (= K42) binding ATP
- S46 (= S43) binding ATP
- T47 (= T44) binding ATP
1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
50% identity, 96% coverage: 8:239/242 of query aligns to 7:250/258 of 1b0uA
5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
41% identity, 97% coverage: 1:234/242 of query aligns to 1:238/615 of 5lilA
- binding adenosine-5'-triphosphate: F13 (= F13), V22 (= V18), S42 (= S38), G43 (= G39), G45 (= G41), K46 (= K42), S47 (= S43), T48 (= T44), Q92 (= Q86), K136 (≠ Q131), Q143 (≠ S138), S145 (= S140), G147 (= G142), Q148 (= Q143)
- binding magnesium ion: S47 (= S43), Q92 (= Q86)
5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
41% identity, 97% coverage: 1:234/242 of query aligns to 1:238/592 of 5lj7A
- binding adenosine-5'-triphosphate: V22 (= V18), S42 (= S38), G43 (= G39), G45 (= G41), K46 (= K42), S47 (= S43), T48 (= T44), Q92 (= Q86), K136 (≠ Q131), Q143 (≠ S138), S145 (= S140), G147 (= G142), Q148 (= Q143)
- binding magnesium ion: S47 (= S43), Q92 (= Q86)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 99% coverage: 3:242/242 of query aligns to 17:252/378 of P69874
- C26 (≠ H12) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F13) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ V31) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ S40) mutation to T: Loss of ATPase activity and transport.
- L60 (= L46) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ V62) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V126) mutation to M: Loss of ATPase activity and transport.
- D172 (= D163) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
38% identity, 99% coverage: 3:242/242 of query aligns to 2:237/358 of 8y5iA
P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 98% coverage: 3:239/242 of query aligns to 1:242/343 of P30750
- 40:46 (vs. 38:44, 86% identical) binding ATP
- E166 (= E164) mutation to Q: Exhibits little ATPase activity.
Sites not aligning to the query:
- 278:283 binding L-methionine
- 295 N→A: Reduces the binding of L-methionine to undetectable levels.
- 295:296 binding L-methionine
3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
36% identity, 98% coverage: 3:239/242 of query aligns to 2:243/344 of 3tuzC
Sites not aligning to the query:
3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
36% identity, 98% coverage: 3:239/242 of query aligns to 2:243/344 of 3tuiC
6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
36% identity, 98% coverage: 3:239/242 of query aligns to 2:243/344 of 6cvlD
- binding phosphothiophosphoric acid-adenylate ester: F12 (= F13), Q14 (vs. gap), I19 (≠ L16), S41 (= S38), G42 (= G39), A43 (≠ S40), G44 (= G41), K45 (= K42), S46 (= S43), T47 (= T44), N141 (≠ S138), S143 (= S140), Q146 (= Q143), H200 (= H196)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 98% coverage: 1:238/242 of query aligns to 1:234/369 of P19566
- L86 (= L90) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P165) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D170) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
41% identity, 88% coverage: 3:216/242 of query aligns to 1:223/232 of 1f3oA
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
36% identity, 98% coverage: 1:238/242 of query aligns to 1:234/371 of P68187
- A85 (≠ N89) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ N111) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A119) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L122) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ D124) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ K129) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G142) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D163) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ P232) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
36% identity, 90% coverage: 1:219/242 of query aligns to 1:220/230 of 6z4wA
Query Sequence
>WP_086510332.1 NCBI__GCF_002151265.1:WP_086510332.1
MSIIEFRNVSKHFGSLKVLDEVDLTIDRGEVVVLIGPSGSGKSTLLRCINGLEMITGGDL
VVDGLSVLAGNARLREIRQEAGMVFQQFNLFPQLTAAENVAFGPRHVRGVNRTQAREQAL
ALLDKVGLKAQAEQYPDSLSGGQQQRVAIARALAVKPKVMLFDEPTSALDPELKQKVLNV
MRTLAEEGMTMVVVTHEMSFARQVGSRLIFMEHGKIAVDGDPRDMISAPTNPRLKDFLRH
VH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory