SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086510419.1 NCBI__GCF_002151265.1:WP_086510419.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8srvB Crystal structure of o-acetyl-l-serine sulfhydrylase a (cysk) from staphylococcus aureus nctc 8325 complexed with a transcriptional repressor (cymr) derived 10 amino acid peptide
48% identity, 64% coverage: 6:317/490 of query aligns to 14:312/314 of 8srvB

query
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8srvB

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binding : T79 (= T70), S80 (= S71), G81 (= G72), T83 (= T74), S127 (≠ A118), A129 (≠ K120), M130 (≠ G121), Q151 (= Q142), F152 (= F143), S215 (≠ A206), G224 (≠ A217), G230 (= G231), G232 (≠ S233), A233 (≠ P234), G234 (≠ D235)

D2Z027 O-ureido-L-serine synthase; Cysteine synthase homolog DscD; O-acetylserine sulfhydrylase; EC 2.6.99.3; EC 2.5.1.47 from Streptomyces lavendulae (see paper)
48% identity, 67% coverage: 1:330/490 of query aligns to 1:316/324 of D2Z027

query
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D2Z027

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K43 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Loss of catalytic activity, no longer binds N6-(pyridoxal phosphate)lysine.
V74 (≠ T74) mutation to T: KM for OAS is 61 mM, KM for H(2)S is unchanged.
Y97 (≠ F97) mutation to F: KM for OAS is unchanged, KM for H(2)S is 0.073 mM.; mutation to M: KM for OAS is 330 mM, KM for H(2)S is 0.084.
S121 (≠ E119) mutation to A: KM for OAS is 140 mM, KM for H(2)S is 0.095 mM.; mutation to M: KM for OAS is 44 mM, KM for H(2)S is 0.20 mM.

3x43A Crystal structure of o-ureido-l-serine synthase (see paper)
49% identity, 67% coverage: 4:330/490 of query aligns to 3:315/316 of 3x43A

query
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3x43A

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active site: K42 (= K43), S264 (= S279)
binding pyridoxal-5'-phosphate: K42 (= K43), N72 (= N73), F175 (= F176), G176 (= G177), T177 (= T178), T178 (≠ G179), T180 (= T181), G220 (= G231), S264 (= S279), P290 (= P305), D291 (= D306)

3x44A Crystal structure of o-ureido-l-serine-bound k43a mutant of o-ureido- l-serine synthase (see paper)
48% identity, 67% coverage: 4:330/490 of query aligns to 3:315/321 of 3x44A

query
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3x44A

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S

V

V

A

-

D

-

M

-

V

I

D

D

V

L

V

V

P

T

V

M

A

D

G

E

D

V

E

T

A

A

L

R

R

D

L

T

A

S

R

R

E

R

L

L

A

A

R

A

K

E

E

E

G

G

I

I

F

F

V

A

G

G

I

I

S
|
S

A

A

G

G

A

A

T

T

L

V

A

A

A

T

A

A

L

L

E

S

V

I

A

A

R

E

R

H

A

A

P

P

A

E

G

G

S

T

H

V

I

L

V

L

C

A

M

M

L

L

P
|
P

D
|
D

T

T

G

G

E

E

R

R

Y

Y

Q

L

S

S

T

T

P

F

L

L

F

F

E

D

G

G

I

V

E

D

D

E

E

G

M

S

N

D

E

D

E

A

E

W

L

L

A

A

active site: A42 (≠ K43), S264 (= S279)
binding (E)-O-(carbamoylamino)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: T69 (= T70), S70 (= S71), N72 (= N73), V73 (≠ T74), S120 (≠ E119), Q141 (= Q142), F175 (= F176), G176 (= G177), T177 (= T178), T178 (≠ G179), T180 (= T181), G220 (= G231), L221 (≠ W232), P223 (= P234), S264 (= S279), P290 (= P305), D291 (= D306)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 66% coverage: 1:322/490 of query aligns to 1:310/310 of P9WP55

query
sites
P9WP55

M

M

T

S

R

I

Y

A

A

E

S

D

I

I

L

T

D

Q

T

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

R

R

L

L

A

R

R

R

L

V

A

T

P

D

P

G

T

A

V

V

-

A

N

D

V

I

H

V

V

A

K

K

V

L

E

E

A

F

F

F

N

N

P

P

M

A

G

N

S

S

V

V

K
|
K

D

D

R

R

M

I

A

G

L

V

A

A

V

M

I

L

E

Q

A

A

E

E

R

Q

S

A

G

G

D

L

L

I

R

K

P

P

G

D

Q

T

T

I

V

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

M

V

V

C

C

A

A

R

A

K

R

G

G

Y

Y

P

R

L

C

V

V

V

L

T

T

M

M

A

P

E

E

S

T

F

M

S

S

L

L

E

E

R

R

R

M

L

L

R

R

F

A

L

Y

G

G

A

A

R

E

V

L

V

I

L

L

T

T

P

P

A

G

A

A

E

D

K

G

G

M

S

S

G

G

M

A

L

I

A

A

K

K

A

A

I

E

E

E

L

L

A

A

E

K

R

T

-

D

H

Q

G

R

Y

Y

F

F

L

V

C

P

R

Q

Q

Q

F

F

E

E

N

N

E

P

A

A

N

N

A

P

E

A

V

I

H

H

S

R

R

V

T

T

A

A

R

E

E

E

I

V

L

W

D

R

D

D

M

T

Q

D

G

G

E

K

P

-

I

V

H

D

A

I

W

V

V

V

S

A

G

G

F

V

G
|
G

T
|
T

G
|
G

T
|
T

L

I

K

T

G

G

V

V

S

A

R

Q

V

V

L

I

K

K

A

E

A

R

D

K

P

P

R

S

I

A

R

R

I

F

V

V

V

A

A

V

E

E

P

P

D

A

N

A

A

S

P

P

L

V

L

L

G

S

S

G

G

G

E

Q

V

K

Q

G

P

-

A

-

G

-

V

-

S

-

H

-

P

-

R

-

F

-

R

-

P

P

H

H

L

P

M

I

Q

Q

G

G

W

I

S

G

P

A

D

G

F

F

I

V

S

P

P

P

L

V

T

L

Q

D

Q

Q

A

-

V

-

A

-

D

D

M

L

V

V

D

D

D

E

V

I

P

T

V

V

A

G

G

N

D

E

E

D

A

A

L

L

R

N

L

V

A

A

R

R

E

R

L

L

A

A

R

R

K

E

E

E

G

G

I

L

F

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

T

A

L

T

A

V

A

A

L

L

E

Q

V

V

A

A

R

R

-

P

A

E

P

N

A

A

G

G

S

K

H

L

I

I

V

V

C

V

M

V

L

L

P

P

D

D

T

F

G

G

E

E

R

R

Y

Y

Q

L

S

S

T

T

P

P

L

L

F

F

E

A

G

D

I

V

E

A

D

D

K44 (= K43) modified: N6-(pyridoxal phosphate)lysine
N74 (= N73) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 177:181) binding pyridoxal 5'-phosphate
S266 (= S279) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
48% identity, 65% coverage: 1:317/490 of query aligns to 1:305/306 of 2q3dA

query
sites
2q3dA

M

M

T

S

R

I

Y

A

A

E

S

D

I

I

L

T

D

Q

T

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

R

R

L

L

A

R

R

R

L

V

A

T

P

D

P

G

T

A

V

V

-

A

N

D

V

I

H

V

V

A

K

K

V

L

E

E

A

F

F

F

N

N

P

P

M

A

G

N

S

S

V

V

K
|
K

D

D

R

R

M

I

A

G

L

V

A

A

V

M

I

L

E

Q

A

A

E

E

R

Q

S

A

G

G

D

L

L

I

R

K

P

P

G

D

Q

T

T

I

V

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

M

V

V

C

C

A

A

R

A

K

R

G

G

Y

Y

P

R

L

C

V

V

V

L

T

T

M

M

A

P

E

E

S

T

F

M

S

S

L

L

E

E

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R

M

L

L

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F

A

L

Y

G

G

A

A

R

E

V

L

V

I

L

L

T

T

P

P

A

G

A

A

E

D

K

G

G

M

S

S

G

G

M

A

L

I

A

A

K

K

A

A

I

E

E

E

L

L

A

A

E

K

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T

-

D

H

Q

G

R

Y

Y

F

F

L

V

C

P

R

Q

Q
|
Q

F

F

E

E

N

N

E

P

A

A

N

N

A

P

E

A

V

I

H

H

S

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R

V

T

T

A

A

R

E

E

E

I

V

L

W

D

R

D

D

M

T

Q

D

G

G

E

K

P

-

I

V

H

D

A

I

W

V

V

V

S

A

G

G

F
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

K

T

G

G

V

V

S

A

R

Q

V

V

L

I

K

K

A

E

A

R

D

K

P

P

R

S

I

A

R

R

I

F

V

V

V

A

A

V

E

E

P

P

D

A

N

A

A

S

P

P

L

V

L

L

G

S

S

G

G

G

E

Q

V

K

Q

G

P

-

A

-

G

-

V

-

S

-

H

-

P

-

R

-

F

-

R

-

P

P

H

H

L

P

M

I

Q

Q

G
|
G

W
x
I

S

G

P

A

D

G

F

F

I

V

S

P

P

P

L

V

T

L

Q

D

Q

Q

A

-

V

-

A

-

D

D

M

L

V

V

D

D

D

E

V

I

P

T

V

V

A

G

G

N

D

E

E

D

A

A

L

L

R

N

L

V

A

A

R

R

E

R

L

L

A

A

R

R

K

E

E

E

G

G

I

L

F

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

T

A

L

T

A

V

A

A

L

L

E

Q

V

V

A

A

R

R

-

P

A

E

P

N

A

A

G

G

S

K

H

L

I

I

V

V

C

V

M

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

Q

L

S

S

T

T

P

P

L

L

F

F

active site: K44 (= K43), S266 (= S279), P293 (= P305)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K43), T71 (= T70), S72 (= S71), N74 (= N73), T75 (= T74), Q144 (= Q142), V177 (≠ F176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (= T181), G222 (= G231), I223 (≠ W232), S266 (= S279), P293 (= P305), D294 (= D306)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
47% identity, 63% coverage: 1:311/490 of query aligns to 1:299/300 of 3zeiA

query
sites
3zeiA

M

M

T

S

R

I

Y

A

A

E

S

D

I

I

L

T

D

Q

T

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

R

R

L

L

A

R

R

R

L

V

A

T

P

D

P

G

T

A

V

V

-

A

N

D

V

I

H

V

V

A

K

K

V

L

E

E

A

F

F

F

N

N

P

P

M

A

G

N

S

S

V

V

K
|
K

D

D

R

R

M

I

A

G

L

V

A

A

V

M

I

L

E

Q

A

A

E

E

R

Q

S

A

G

G

D

L

L

I

R

K

P

P

G

D

Q

T

T

I

V

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

M

V

V

C

C

A

A

R

A

K

R

G

G

Y

Y

P

R

L

C

V

V

V

L

T

T

M

M

A

P

E

E

S

T

F

M

S

S

L

L

E

E

R

R

R

M

L

L

R

R

F

A

L

Y

G

G

A

A

R

E

V

L

V

I

L

L

T

T

P

P

A

G

A

A

E

D

K

G

G

M

S

S

G

G

M

A

L
x
I

A

A

K

K

A

A

I

E

E

E

L

L

A

A

E

K

R

T

-

D

H

Q

G

R

Y

Y

F

F

L

V

C

P

R

Q

Q
|
Q

F
|
F

E

E

N

N

E

P

A

A

N

N

A

P

E

A

V

I

H

H

S

R

R

V

T

T

A

A

R

E

E

E

I

V

L

W

D

R

D

D

M

T

Q

D

G

G

E

K

P

-

I

V

H

D

A

I

W

V

V

V

S

A

G

G

F
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

K

T

G

G

V

V

S

A

R

Q

V

V

L

I

K

K

A

E

A

R

D

K

P

P

R

S

I

A

R

R

I

F

V

V

V

A

A

V

E

E

P

P

D

A

N

A

A

S

P

P

L

V

L

L

G

S

S

G

G

G

E

Q

V
x
K

Q

G

P

-

A

-

G

-

V

-

S

-

H

-

P

-

R

-

F

-

R

-

P

P

H

H

L

P

M

I

Q

Q

G
|
G

W

I

S

G

P
x
A

D

G

F
|
F

I

V

S

P

P

P

L

V

T

L

Q

D

Q

Q

A

-

V

-

A

-

D

D

M

L

V

V

D

D

D

E

V

I

P

T

V

V

A

G

G

N

D

E

E

D

A

A

L

L

R

N

L

V

A

A

R

R

E

R

L

L

A

A

R

R

K

E

E

E

G

G

I

L

F

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

T

A

L

T

A

V

A

A

L

L

E

Q

V

V

A

A

R

R

-

P

A

E

P

N

A

A

G

G

S

K

H

L

I

I

V

V

C

V

M

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

active site: K44 (= K43), S266 (= S279), P293 (= P305)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T70), S72 (= S71), I126 (≠ L125), Q144 (= Q142), F145 (= F143), K215 (≠ V214), G222 (= G231), A225 (≠ P234), F227 (= F236)
binding pyridoxal-5'-phosphate: K44 (= K43), N74 (= N73), V177 (≠ F176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (= T181), G222 (= G231), S266 (= S279), P293 (= P305), D294 (= D306)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
47% identity, 63% coverage: 1:311/490 of query aligns to 1:299/300 of 2q3cA

query
sites
2q3cA

M

M

T

S

R

I

Y

A

A

E

S

D

I

I

L

T

D

Q

T

L

I

I

G

G

R

R

T

T

P

P

L

L

V

V

R

R

L

L

A

R

R

R

L

V

A

T

P

D

P

G

T

A

V

V

-

A

N

D

V

I

H

V

V

A

K

K

V

L

E

E

A

F

F

F

N

N

P

P

M

A

G

N

S

S

V

V

K
|
K

D

D

R

R

M

I

A

G

L

V

A

A

V

M

I

L

E

Q

A

A

E

E

R

Q

S

A

G

G

D

L

L

I

R

K

P

P

G

D

Q

T

T

I

V

I

I

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

M

V

V

C

C

A

A

R

A

K

R

G

G

Y

Y

P

R

L

C

V

V

V

L

T

T

M

M

A

P

E

E

S

T

F

M

S

S

L

L

E

E

R

R

R

M

L

L

R

R

F

A

L

Y

G

G

A

A

R

E

V

L

V

I

L

L

T

T

P

P

A

G

A

A

E

D

K

G

G
x
M

S

S

G

G

M

A

L

I

A

A

K

K

A

A

I

E

E

E

L

L

A

A

E

K

R

T

-

D

H

Q

G

R

Y

Y

F

F

L

V

C

P

R

Q

Q
|
Q

F

F

E

E

N

N

E

P

A

A

N

N

A

P

E

A

V

I

H

H

S

R

R

V

T

T

A

A

R

E

E

E

I

V

L

W

D

R

D

D

M

T

Q

D

G

G

E

K

P

-

I

V

H

D

A

I

W

V

V

V

S

A

G

G

F

V

G

G

T

T

G

G

T

T

L

I

K

T

G

G

V

V

S

A

R

Q

V

V

L

I

K

K

A

E

A

R

D

K

P

P

R

S

I

A

R

R

I

F

V

V

V

A

A

V

E

E

P

P

D

A

N

A

A

S

P

P

L

V

L

L

G

S

S

G

G

G

E

Q

V
x
K

Q

G

P

-

A

-

G

-

V

-

S

-

H

-

P

-

R

-

F

-

R

-

P

P

H

H

L

P

M

I

Q

Q

G
|
G

W

I

S

G

P
x
A

D

G

F

F

I

V

S

P

P

P

L

V

T

L

Q

D

Q

Q

A

-

V

-

A

-

D

D

M

L

V

V

D

D

D

E

V

I

P

T

V

V

A

G

G

N

D

E

E

D

A

A

L

L

R

N

L

V

A

A

R

R

E

R

L

L

A

A

R

R

K

E

E

E

G

G

I

L

F

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

T

A

L

T

A

V

A

A

L

L

E

Q

V

V

A

A

R

R

-

P

A

E

P

N

A

A

G

G

S

K

H

L

I

I

V

V

C

V

M

V

L

L

P
|
P

D

D

T

F

G

G

E

E

R

R

Y

Y

active site: K44 (= K43), S266 (= S279), P293 (= P305)
binding : T71 (= T70), S72 (= S71), G73 (= G72), T75 (= T74), M122 (≠ G121), Q144 (= Q142), K215 (≠ V214), G222 (= G231), A225 (≠ P234)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
46% identity, 64% coverage: 9:320/490 of query aligns to 10:309/310 of 5xoqA

query
sites
5xoqA

D

E

T

S

I

I

G

G

R

R

T

T

P

P

L

L

V

V

R

Q

L

I

A

N

R

R

L

L

-

T

A

A

P

G

P

L

T

S

V

S

N

R

V

V

H

L

V

A

K

K

V

I

E

E

A

G

F

R

N

N

P

P

M

A

G

Y

S

S

V

V

K

K

D

C

R

R

M

I

A

G

L

A

A

A

V

M

I

I

E

W

A

D

A

A

E

E

R

Q

S

S

G

G

D

K

L

L

R

K

P

P

G

G

Q

M

T

H

V

V

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

F

V

V

C

C

A

A

R

A

K

R

G

G

Y

Y

P

K

L

L

V

T

V

L

T

T

M

M

A

P

E

E

S

T

F

M

S

S

L

I

E

E

R

R

R

M

L

M

L

L

R

K

F

S

L

F

G

G

A

A

R

D

V

L

V

V

L

L

T

T

P

P

A

G

A

A

E

D

K

G

G
x
M

S

K

G

G

M

A

L

I

A

S

K

K

A

A

I

E

E

E

L

L

A

A

E

A

R

Q

H

P

G

G

Y

W

F

F

L

I

C

P

R

Q

Q
|
Q

F

F

E

K

N

N

E

P

A

A

N

N

A

P

E

A

V

I

H

H

S

V

R

K

T

T

A

G

R

P

E

E

I

I

L

W

D

N

D

D

M

T

Q

E

G

G

E

Q

P

-

I

V

H

D

A

V

W

F

V

V

S

A

G

G

F

V

G

G

T

T

G

G

T

T

L

I

K

T

G

G

V

V

S

A

R

R

V

F

L

L

K

K

A

H

A

E

D

K

P

K

R

H

-

P

I

V

R

H

I

V

V

V

V

A

A

V

E

E

P

P

D

A

N

A

A

S

P

P

L

V

L

L

G

A

S

G

G

G

E

-

V

-

Q

-

P

P

A

A

G

G

V

-

S

-

H

-

P

-

R

-

F

-

R

-

P
x
R

H
|
H

L

K

M

I

Q
|
Q

G
|
G

W

I

S

G

P
x
A

D

G

F

F

I

V

S

P

P

D

L

T

T

F

Q

D

Q

R

A

S

V

V

A

-

D

-

M

-

V

V

D

D

D

E

V

I

V

L

P

S

V

V

A

T

G

D

D

D

E

E

A

A

L

I

R

E

L

T

A

A

R

R

E

K

L

L

A

A

R

M

K

E

E

E

G

G

I

I

F

S

V

C

G

G

I

I

S

S

A

C

G

G

A

A

T

A

L

M

A

A

A

G

A

A

L

L

E

K

V

V

A

A

R

A

R

R

A

P

P

E

-

F

A

A

G

G

S

K

H

T

I

I

V

V

C

T

M

V

L

L

P

P

D

D

T

A

G

G

E

E

R

R

Y

Y

Q

L

S

S

T

T

P

A

L

L

F

F

E

E

G

N

I

L

binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), M123 (≠ G121), Q144 (= Q142), R218 (≠ P226), H219 (= H227), Q222 (= Q230), G223 (= G231), A226 (≠ P234)

3t4pA Crystal structure of o-acetyl serine sulfhydrylase from leishmania donovani in complex with designed tetrapeptide (see paper)
42% identity, 65% coverage: 6:324/490 of query aligns to 13:319/319 of 3t4pA

query
sites
3t4pA

S

S

I

I

L

D

D

Q

T

L

I

I

G

G

R

Q

T

T

P

P

L

A

V

L

R

Y

L

L

A

N

R

K

L

L

A

N

P

N

P

T

T

K

V

A

N

K

V

V

H

V

V

L

K

K

V

M

E

E

A

C

F

E

N

N

P

P

M

M

G

A

S

S

V

V

K
|
K

D

D

R

R

M

L

A

G

L

F

A

A

V

I

I

Y

E

D

A

K

A

A

E

E

R

K

S

E

G

G

D

K

L

L

R

I

P

P

G

G

Q

K

T

S

-

I

V

V

I

V

E

E

A

S

T
x
S

S
|
S

G
|
G

N

N

T
|
T

G

G

I

V

G

S

L

L

A

A

M

H

V

L

C

G

A

A

R

I

K

R

G

G

Y

Y

P

K

L

V

V

I

T

T

M

M

A

P

E

E

S

S

F

M

S

S

L

L

E

E

R

R

R

C

L

L

R

R

F

I

L

F

G

G

A

A

R

E

V

V

V

I

L

L

T

T

P

P

A

A

-

L

-

G

E
x
M

K

K

G

G

S

A

G

V

M

A

L

M

A

A

K

K

A

K

I

I

E

-

L

V

A

A

E

A

R

N

H

P

G

N

Y

A

F

V

L

L

C

A

R

D

Q
|
Q

F
|
F

E

A

N

T

E

K

A

Y

N

N

A

A

E

L

V

I

H

H

S

E

R

E

T

T

A

G

R

P

E

E

I

I

L

W

D

E

D

Q

M

T

Q

N

G

-

E

H

P

N

I

V

H

D

A

C

W

F

V

I

S

A

G

G

F

V

G

G

T

T

G

G

T

T

L

L

K

T

G

G

V

V

S

A

R

R

V

A

L

L

K

K

A

K

A

M

D

G

P

S

R

H

I

A

R

R

I

I

V

V

V

A

A

V

E

E

P

P

D

T

N

E

A

S

P

P

L

V

L

L

G

S

S

G

G

G

E

-

V

-

Q

K

P

P

A
x
G

G

-

V

-

S

-

H

-

P

-

R

-

F

-

R

-

P
|
P

H
|
H

L

K

M

I

Q

Q

G
|
G

W

I

S
x
G

P
|
P

D

G

F

F

I

V

S

P

P

D

L

V

T

L

Q

D

Q

R

A

S

V

-

A

-

D

-

M

L

V

I

D

D

D

E

V

V

V

L

P

C

V

V

A

A

G

G

D

D

E

D

A

A

L

I

R

E

L

T

A

A

R

L

E

K

L

L

A

T

R

R

K

S

E

D

G

G

I

V

F

F

V

C

G

G

I

F

S
|
S

A

G

G

G

A

A

T

N

L

V

A

Y

A

A

L

L

E

K

V

I

A

A

R

E

R

R

A

P

P

E

A

M

-

E

G

G

S

K

H

T

I

I

V

V

C

T

M

V

L

I

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

Q

L

S

S

T

T

P

T

L

L

F

Y

E

R

G

S

I

V

E

R

D

D

E

E

M

V

active site: K50 (= K43), S273 (= S279)
binding : S78 (≠ T70), S79 (= S71), G80 (= G72), T82 (= T74), M129 (≠ E119), Q151 (= Q142), F152 (= F143), G223 (≠ A217), P224 (= P226), H225 (= H227), G229 (= G231), G231 (≠ S233), P232 (= P234)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
40% identity, 66% coverage: 7:331/490 of query aligns to 7:318/318 of 4lmaA

query
sites
4lmaA

I

V

L

T

D

E

T

L

I

V

G

G

R

R

T

T

P

P

L

L

V

V

R

Q

L

L

A

N

R

R

L

I

A

-

P

-

P

P

T

Q

V

A

N

E

-

G

-

C

-

L

-

G

-

R

V

I

H

V

V

M

K

K

V

L

E

E

A

G

F

M

N

N

P

P

M

A

G

A

S

S

V

V

K
|
K

D

D

R

R

M

I

A

G

L

T

A

H

V

M

I

I

E

N

A

S

A

A

E

E

R

K

S

A

G

G

D

L

L

I

R

N

P

P

G

E

Q

T

T

T

V

V

-

L

I

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

M

V

T

C

A

A

A

R

A

K

K

G

G

Y

Y

P

R

L

L

V

I

V

L

T

T

M

M

A

P

E

E

S

T

F

M

S

S

L

L

E

E

R

R

R

A

L

M

L

L

R

K

F

A

L

Y

G

G

A

A

R

T

V

L

V

E

L

L

T

T

P

P

A

G

A

S

E

Q

K

G

G

M

S

K

G

G

M

A

L

I

A

L

K

R

A

A

I

Q

E

Q

L

I

A

V

E

D

R

S

-

I

H

P

G

G

Y

A

F

Y

L

M

C

L

R

Q

Q

Q

F

F

E

R

N

N

E

P

A

S

N

N

A

P

E

E

V

I

H

H

S

R

R

L

T

T

A

A

R

E

E

E

I

I

L

W

D

Q

D

D

M

T

Q

E

G

G

E

Q

P

-

I

V

H

D

A

F

W

I

V

V

S

A

G

G

F

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

K

T

G

G

V

V

S

A

R

E

V

V

L

I

K

K

A

S

A

R

D

K

P

P

R

S

I

F

R

Q

I

V

V

V

V

A

A

V

E

E

P

P

D

F

N

N

A

S

P

P

L

V

L

I

G

S

S

G

G

G

E

-

V

-

Q

N

P

P

A

G

G

-

V

-

S

-

H

-

P

-

R

-

F

-

R

-

P

P

H

H

L

K

M

I

Q

Q

G
|
G

W

I

S

G

P

A

D

G

F

F

I

I

S

P

P

-

L

-

T

-

Q

-

Q

E

A

V

V

L

A

R

A

T

D

D

M

L

V

I

D

D

D

E

V

V

V

I

P

T

V

V

A

S

G

D

D

E

E

E

A

A

L

F

R

Q

L

F

A

G

R

R

E

R

L

L

A

A

R

K

K

E

E

E

G

G

I

L

F

L

V

S

G

G

I

I

S
|
S

A

S

G

G

A

A

T

N

L

L

A

C

A

A

L

I

E

Q

V

L

A

A

R

Q

R

R

-

P

A

E

P

N

A

E

G

G

S

K

H

L

I

I

V

V

C

V

M

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

Q

L

S

S

T

T

P

L

L

M

F

F

E

Q

G

N

I

I

E

E

D

-

E

-

M

-

N

-

E

E

E

R

E

E

L

L

A

T

L

L

active site: K46 (= K43), S269 (= S279)
binding pyridoxal-5'-phosphate: K46 (= K43), N77 (= N73), G181 (= G177), T182 (= T178), G183 (= G179), T185 (= T181), G225 (= G231), S269 (= S279), P296 (= P305)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
42% identity, 63% coverage: 11:317/490 of query aligns to 7:301/302 of 2efyA

query
sites
2efyA

I

I

G

G

R

K

T

T

P

P

L

V

V

V

R

R

L

L

A

A

R

K

L

V

A

V

P

E

P

P

T

D

V

M

-

A

N

E

V

V

H

W

V

V

K

K

V

L

E

E

A

G

F

L

N

N

P

P

M

G

G

G

S

S

V

I

K
|
K

D

D

R

R

M

P

A

A

L

W

A

Y

V

M

I

I

E

K

A

D

A

A

E

E

R

E

S

R

G

G

D

I

L

L

R

R

P

P

G

G

-

S

-

G

Q

Q

T

V

V

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

V

I

C

A

A

A

R

S

K

R

G

G

Y

Y

P

R

L

L

V

I

V

L

T

T

M

M

A

P

E

A

S

Q

F

M

S

S

L

E

E

E

R

R

R

K

R

R

L

V

L

L

R

K

F

A

L

F

G

G

A

A

R

E

V

L

V

V

L

L

T

T

P

D

A

P

A

E

E

R

K

R

G

M

S

L

G

A

M

A

L

R

A

E

K

E

A

A

I

L

E

R

L

L

A

K

E

E

R

E

H

L

G

G

Y

A

F

F

L

M

C

P

R

D

Q
|
Q

F

F

E

K

N

N

E

P

A

A

N

N

A

V

E

R

V

A

H

H

S

Y

R

E

T

T

A

G

R

P

E

E

I

L

L

Y

D

E

D

A

M

L

Q

E

G

G

E

R

P

-

I

I

H

D

A

A

W

F

V

V

S
x
Y

G

G

F

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

K

T

G

G

V

V

S

G

R

R

V

Y

L

L

K

K

A

E

A

R

D

I

P

P

R

H

I

V

R

K

I

V

V

I

V

A

A

V

E
|
E

P

P

D

A

N

R

A
x
S

P

N

L

V

L

L

G

S

S

G

G

G

E

-

V

-

Q

-

P

-

A

-

G

-

V

-

S

-

H

-

P

-

R

K

F

M

R

G

P

Q

H

H

L

G

M

F

Q

Q

G
|
G

W
x
M

S

G

P
|
P

D

G

F

F

I

I

S

P

P

-

L

-

T

-

Q

-

Q

E

A

N

V

L

A

D

A

L

D

S

M

L

V

L

D

D

D

G

V

V

V

I

P

Q

V

V

A

W

G

E

D

E

E

D

A

A

L

F

R

P

L

L

A

A

R

R

E

R

L

L

A

A

R

R

K

E

E

E

G

G

I

L

F

F

V

L

G

G

I

M

S
|
S

A

S

G

G

A

G

T

I

L

V

A

W

A

A

L

L

E

Q

V

V

A

A

R

R

R

E

A

L

P

G

A

P

G

G

S

K

H

R

I

V

V

A

C

C

M

I

L

S

P
|
P

D
|
D

T

G

G

G

E

W

R

K

Y

Y

Q

L

S

S

T

T

P

P

L

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (= E202), S204 (≠ A206), S263 (= S279)
binding 5-oxohexanoic acid: T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q142), G175 (= G177), G219 (= G231), M220 (≠ W232), P222 (= P234)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S174), G175 (= G177), T176 (= T178), G177 (= G179), T179 (= T181), G219 (= G231), S263 (= S279), P289 (= P305), D290 (= D306)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
42% identity, 63% coverage: 11:317/490 of query aligns to 7:301/302 of 2ecqA

query
sites
2ecqA

I

I

G

G

R

K

T

T

P

P

L

V

V

V

R

R

L

L

A

A

R

K

L

V

A

V

P

E

P

P

T

D

V

M

-

A

N

E

V

V

H

W

V

V

K

K

V

L

E

E

A

G

F

L

N

N

P

P

M

G

G

G

S

S

V

I

K
|
K

D

D

R

R

M

P

A

A

L

W

A

Y

V

M

I

I

E

K

A

D

A

A

E

E

R

E

S

R

G

G

D

I

L

L

R

R

P

P

G

G

-

S

-

G

Q

Q

T

V

V

I

I

V

E

E

A

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

V

I

C

A

A

A

R

S

K

R

G

G

Y

Y

P

R

L

L

V

I

V

L

T

T

M

M

A

P

E

A

S

Q

F

M

S

S

L

E

E

E

R

R

R

K

R

R

L

V

L

L

R

K

F

A

L

F

G

G

A

A

R

E

V

L

V

V

L

L

T

T

P

D

A

P

A

E

E

R

K

R

G

M

S

L

G

A

M

A

L

R

A

E

K

E

A

A

I

L

E

R

L

L

A

K

E

E

R

E

H

L

G

G

Y

A

F

F

L

M

C

P

R

D

Q
|
Q

F

F

E

K

N

N

E

P

A

A

N

N

A

V

E

R

V

A

H

H

S

Y

R

E

T

T

A

G

R

P

E

E

I

L

L

Y

D

E

D

A

M

L

Q

E

G

G

E

R

P

-

I

I

H

D

A

A

W

F

V

V

S
x
Y

G
|
G

F

S

G
|
G

T
|
T

G

G

T
|
T

L

I

K

T

G

G

V

V

S

G

R

R

V

Y

L

L

K

K

A

E

A

R

D

I

P

P

R

H

I

V

R

K

I

V

V

I

V

A

A

V

E
|
E

P

P

D

A

N

R

A
x
S

P

N

L

V

L

L

G

S

S

G

G

G

E

-

V

-

Q

-

P

-

A

-

G

-

V

-

S

-

H

-

P

-

R

K

F

M

R

G

P

Q

H

H

L

G

M

F

Q

Q

G
|
G

W

M

S

G

P

P

D

G

F

F

I

I

S

P

P

-

L

-

T

-

Q

-

Q

E

A

N

V

L

A

D

A

L

D

S

M

L

V

L

D

D

D

G

V

V

V

I

P

Q

V

V

A

W

G

E

D

E

E

D

A

A

L

F

R

P

L

L

A

A

R

R

E

R

L

L

A

A

R

R

K

E

E

E

G

G

I

L

F

F

V

L

G

G

I

M

S
|
S

A

S

G

G

A

G

T

I

L

V

A

W

A

A

L

L

E

Q

V

V

A

A

R

R

R

E

A

L

P

G

A

P

G

G

S

K

H

R

I

V

V

A

C

C

M

I

L

S

P
|
P

D

D

T

G

G

G

E

W

R

K

Y

Y

Q

L

S

S

T

T

P

P

L

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (= E202), S204 (≠ A206), S263 (= S279)
binding (3s)-3-hydroxybutanoic acid: K40 (= K43), G71 (= G72), T73 (= T74), Q141 (= Q142), G219 (= G231)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S174), G173 (= G175), G175 (= G177), T176 (= T178), T179 (= T181), G219 (= G231), S263 (= S279), P289 (= P305)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
42% identity, 63% coverage: 11:317/490 of query aligns to 7:301/302 of 2ecoA

query
sites
2ecoA

I

I

G

G

R

K

T

T

P

P

L

V

V

V

R

R

L

L

A

A

R

K

L

V

A

V

P

E

P

P

T

D

V

M

-

A

N

E

V

V

H

W

V

V

K

K

V

L

E

E

A

G

F

L

N

N

P

P

M

G

G

G

S

S

V

I

K
|
K

D

D

R

R

M

P

A

A

L

W

A

Y

V

M

I

I

E

K

A

D

A

A

E

E

R

E

S

R

G

G

D

I

L

L

R

R

P

P

G

G

-

S

-

G

Q

Q

T

V

V

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

V

I

C

A

A

A

R

S

K

R

G

G

Y

Y

P

R

L

L

V

I

V

L

T

T

M

M

A

P

E

A

S

Q

F

M

S

S

L

E

E

E

R

R

R

K

R

R

L

V

L

L

R

K

F

A

L

F

G

G

A

A

R

E

V

L

V

V

L

L

T

T

P

D

A

P

A

E

E

R

K

R

G

M

S

L

G

A

M

A

L

R

A

E

K

E

A

A

I

L

E

R

L

L

A

K

E

E

R

E

H

L

G

G

Y

A

F

F

L

M

C

P

R

D

Q
|
Q

F

F

E

K

N

N

E

P

A

A

N

N

A

V

E

R

V

A

H

H

S

Y

R

E

T

T

A

G

R

P

E

E

I

L

L

Y

D

E

D

A

M

L

Q

E

G

G

E

R

P

-

I

I

H

D

A

A

W

F

V

V

S
x
Y

G

G

F

S

G
|
G

T
|
T

G

G

T
|
T

L

I

K

T

G

G

V

V

S

G

R

R

V

Y

L

L

K

K

A

E

A

R

D

I

P

P

R

H

I

V

R

K

I

V

V

I

V

A

A

V

E
|
E

P

P

D

A

N

R

A
x
S

P

N

L

V

L

L

G

S

S

G

G

G

E

-

V

-

Q

-

P

-

A

-

G

-

V

-

S

-

H

-

P

-

R

K

F

M

R

G

P

Q

H

H

L

G

M

F

Q

Q

G
|
G

W

M

S

G

P

P

D

G

F

F

I

I

S

P

P

-

L

-

T

-

Q

-

Q

E