SitesBLAST
Comparing WP_086510444.1 NCBI__GCF_002151265.1:WP_086510444.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
49% identity, 98% coverage: 2:288/292 of query aligns to 14:296/308 of P27305
- E55 (= E43) binding L-glutamate
- Y182 (= Y171) binding L-glutamate
- R200 (= R189) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
49% identity, 98% coverage: 2:288/292 of query aligns to 2:282/290 of 4a91A
- active site: S11 (≠ T11), K229 (= K230)
- binding glutamic acid: R7 (= R7), A9 (= A9), S11 (≠ T11), E43 (= E43), Y170 (= Y171), R188 (= R189), L192 (= L193)
- binding zinc ion: C99 (= C99), C101 (= C101), Y113 (= Y112), C117 (= C116)
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
35% identity, 80% coverage: 5:237/292 of query aligns to 4:247/468 of 8i9iA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
35% identity, 80% coverage: 5:237/292 of query aligns to 4:247/471 of P04805
- C98 (= C99) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C101) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C116) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ G118) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ P120) mutation to Q: No change in activity or in zinc content.
- H131 (≠ E122) mutation to Q: No change in activity or in zinc content.
- H132 (vs. gap) mutation to Q: No change in activity or in zinc content.
- C138 (≠ L127) mutation to S: No change in activity or in zinc content.
- S239 (= S229) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
37% identity, 80% coverage: 5:237/292 of query aligns to 4:232/380 of 4g6zA
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
31% identity, 98% coverage: 5:290/292 of query aligns to 4:310/485 of Q8DLI5
- R6 (= R7) binding L-glutamate
- Y192 (= Y171) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
31% identity, 98% coverage: 5:290/292 of query aligns to 3:309/484 of 2cfoA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
32% identity, 80% coverage: 5:237/292 of query aligns to 3:260/485 of 4griB
- active site: S9 (≠ T11), K253 (= K230)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (≠ T11), E41 (= E43), Y194 (= Y171), R212 (= R189), W216 (≠ L193)
- binding zinc ion: C105 (= C99), C107 (= C101), Y128 (≠ W115), C132 (= C116)
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
32% identity, 80% coverage: 5:237/292 of query aligns to 104:342/564 of 3al0C
- active site: S110 (≠ T11), K335 (= K230)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (≠ A23), E142 (= E43), Y276 (= Y171), R294 (= R189), G295 (= G190), D297 (= D192), H298 (≠ L193), L324 (= L219), I325 (= I220), L333 (= L228)
- binding : T144 (≠ I45), D145 (= D46), R148 (= R49), Y208 (= Y97), P213 (≠ S102), K252 (≠ F147), M255 (≠ Q150), I266 (≠ V161), K269 (≠ R164), S270 (≠ K165), Y276 (= Y171), D297 (= D192), H298 (≠ L193), L299 (= L194), S300 (≠ D195), N301 (= N196), K304 (≠ W199), R330 (≠ G225), P332 (≠ K227)
Sites not aligning to the query:
- binding : 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
35% identity, 79% coverage: 7:237/292 of query aligns to 5:253/468 of 2cv2A
- active site: K246 (= K230)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (≠ T11), G17 (= G19), I21 (≠ A23), E41 (= E43), Y187 (= Y171), R205 (= R189), A206 (≠ G190), E208 (≠ D192), W209 (≠ L193), L235 (= L219), L236 (≠ I220)
- binding : S9 (≠ T11), T43 (≠ I45), D44 (= D46), R47 (= R49), V145 (≠ A128), R163 (≠ F147), Y168 (≠ F152), E172 (= E156), V177 (= V161), K180 (≠ R164), S181 (≠ K165), Y187 (= Y171), E207 (≠ F191), E208 (≠ D192), W209 (≠ L193), V211 (≠ D195), R237 (≠ V221), K241 (≠ G225)
Sites not aligning to the query:
- binding : 272, 273, 274, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 432, 435, 442, 443, 444, 446, 447, 448
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
35% identity, 79% coverage: 7:237/292 of query aligns to 5:253/468 of 2cv1A
- active site: K246 (= K230)
- binding adenosine-5'-triphosphate: P8 (= P10), S9 (≠ T11), G17 (= G19), T18 (≠ S20), I21 (≠ A23), R47 (= R49), A206 (≠ G190), W209 (≠ L193), L235 (= L219), L236 (≠ I220)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (= E43), Y187 (= Y171), R205 (= R189), W209 (≠ L193)
- binding : S9 (≠ T11), E41 (= E43), T43 (≠ I45), D44 (= D46), R47 (= R49), V145 (≠ A128), R163 (≠ F147), V166 (≠ Q150), E172 (= E156), V177 (= V161), K180 (≠ R164), S181 (≠ K165), Y187 (= Y171), E207 (≠ F191), E208 (≠ D192), W209 (≠ L193), V211 (≠ D195), R237 (≠ V221), K241 (≠ G225), K243 (= K227)
Sites not aligning to the query:
- binding : 273, 274, 276, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
35% identity, 79% coverage: 7:237/292 of query aligns to 5:253/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
35% identity, 79% coverage: 7:237/292 of query aligns to 5:253/468 of 1n78A
- active site: K246 (= K230)
- binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (≠ T11), G17 (= G19), T18 (≠ S20), I21 (≠ A23), E41 (= E43), Y187 (= Y171), N191 (≠ V175), R205 (= R189), A206 (≠ G190), E208 (≠ D192), W209 (≠ L193), L235 (= L219), L236 (≠ I220)
- binding : S9 (≠ T11), T43 (≠ I45), D44 (= D46), R47 (= R49), V145 (≠ A128), R163 (≠ F147), V166 (≠ Q150), Y168 (≠ F152), E172 (= E156), V177 (= V161), K180 (≠ R164), S181 (≠ K165), Y187 (= Y171), E207 (≠ F191), E208 (≠ D192), W209 (≠ L193), L210 (= L194), V211 (≠ D195), R237 (≠ V221), K241 (≠ G225)
Sites not aligning to the query:
- binding : 273, 274, 282, 297, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
35% identity, 79% coverage: 7:237/292 of query aligns to 5:253/468 of 1j09A
- active site: K246 (= K230)
- binding adenosine-5'-triphosphate: H15 (= H17), E208 (≠ D192), L235 (= L219), L236 (≠ I220), K243 (= K227), I244 (≠ L228), S245 (= S229), K246 (= K230), R247 (≠ Q231)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (≠ T11), E41 (= E43), Y187 (= Y171), N191 (≠ V175), R205 (= R189), W209 (≠ L193)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 79% coverage: 7:237/292 of query aligns to 5:253/468 of P27000
Sites not aligning to the query:
- 358 R→Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
35% identity, 79% coverage: 7:237/292 of query aligns to 5:253/468 of 1g59A
- binding : D44 (= D46), R45 (≠ P47), A46 (≠ P48), R47 (= R49), P109 (≠ R103), V145 (≠ A128), R163 (≠ F147), V166 (≠ Q150), E172 (= E156), V177 (= V161), K180 (≠ R164), S181 (≠ K165), D182 (= D166), E207 (≠ F191), E208 (≠ D192), R237 (≠ V221), K241 (≠ G225), T242 (≠ Q226), K243 (= K227)
Sites not aligning to the query:
- binding : 273, 274, 282, 299, 300, 303, 304, 309, 312, 319, 357, 358, 417, 426, 427, 432, 435, 442, 443, 444, 445, 446, 447, 448
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
29% identity, 97% coverage: 1:284/292 of query aligns to 1:301/488 of 8vc5A