SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086510675.1 NCBI__GCF_002151265.1:WP_086510675.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4hpnA Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
72% identity, 99% coverage: 1:375/378 of query aligns to 1:375/378 of 4hpnA

query
sites
4hpnA

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active site: F19 (= F19), G50 (= G50), R53 (≠ Q53), T134 (= T134), K164 (= K164), K166 (= K166), D194 (= D194), N196 (= N196), E220 (= E220), G245 (= G245), E246 (= E246), T247 (= T247), Q267 (= Q267), D269 (= D269), H296 (= H296), V297 (= V297), W298 (= W298), R320 (= R320), E329 (= E329), F330 (= F330), H334 (≠ E334)
binding calcium ion: D194 (= D194), D209 (≠ R209), E220 (= E220), G237 (≠ D237), E246 (= E246)

5olcC Crystal structure of the 3,6-anhydro-d-galactonate cycloisomerase from zobellia galactanivorans (see paper)
40% identity, 98% coverage: 2:373/378 of query aligns to 2:348/351 of 5olcC

query
sites
5olcC

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active site: K148 (= K164), K150 (= K166), D178 (= D194), N180 (= N196), E204 (= E220), G229 (= G245), E230 (= E246), D253 (= D269), H280 (= H296), E304 (= E329), E309 (= E334)
binding magnesium ion: D178 (= D194), E204 (= E220), E230 (= E246)

3sjnA Crystal structure of enolase spea_3858 (target efi-500646) from shewanella pealeana with magnesium bound
36% identity, 90% coverage: 33:373/378 of query aligns to 29:370/373 of 3sjnA

query
sites
3sjnA

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active site: S46 (vs. gap), L49 (vs. gap), T139 (≠ S136), K165 (= K166), G167 (= G168), M171 (vs. gap), D198 (= D194), A200 (= A195), E225 (= E220), I247 (≠ V242), G250 (= G245), E251 (= E246), S252 (≠ T247), Q272 (= Q267), D274 (= D269), H301 (= H296), G302 (≠ V297), F303 (≠ W298), M325 (≠ L328), E326 (= E329), Q329 (≠ R332), S331 (≠ E334)
binding magnesium ion: D198 (= D194), E225 (= E220), E251 (= E246)

3ozmD Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
32% identity, 99% coverage: 1:374/378 of query aligns to 2:373/381 of 3ozmD

query
sites
3ozmD

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A

A

L

L

W

W

D

D

I

L

K

K

G

G

K

R

R

A

F

M

G

N

V

Q

P

P

I

I

S

Y

T

Q

L

L

G

G

R

K

F

F

R

H

D

T

-

R

S

G

V

V

R

R

A

A

Y

Y

A

A

T
x
S

G

S

S

I

F

Y

R

W

R

D

A

L

G

T

V

P

D

D

R

Q

V

A

E

A

D

D

N

-

A

-

R

-

E

E

V

L

A

A

G

G

Y

W

R

V

R

E

E

Q

G

G

F

F

H

T

A

A

V

A

K
|
K

I

L

K
|
K

I

V

G

G

F

R

G

A

V

P

D

R

E

K

D

D

L

A

R

A

V

N

I

L

E

R

A

A

V

M

R

R

E

Q

S

R

I

V

G

G

P

A

D

D

M

V

R

E

L

I

M

L

I

V

D
|
D

A

A

N
|
N

H

Q

G

S

Y

L

D

G

Y

R

L

H

E

D

A

A

V

L

E

A

V

M

G

L

R

R

R

I

A

L

A

D

K

E

F

A

G

G

I

C

D

Y

W

W

F

F

E
|
E

E

E

P

P

V

L

L

S

P

I

E

D

H

D

V

I

S

E

A

G

Y

H

R

R

A

I

V

L

R

R

A

A

D

Q

-

G

Q

T

P

P

I

V

P

R

V

I

A

A

G

T

G
|
G

E
|
E

T
x
N

W

L

H

Y

G

T

R

R

Y

N

A

A

M

F

H

N

E

D

P

Y

L

I

A

R

T

N

R

D

A

A

V

I

D

D

I

V

I

L

Q
|
Q

P

A

D
|
D

I

A

C

S

G

R

V

A

G

G

F

I

T

T

E

E

I

A

R

L

R

A

V

I

A

S

D

A

M

S

A

A

L

S

H

A

G

H

V

L

R

A

L

W

V

N

P

P

H
|
H

V
x
T

W
x
F

G

N

T

D

A

I

V

I

C

T

L

V

A

A

S

N

L

L

Q

H

F

L

M

V

A

A

L

-

P

-

N

-

P

-

R

-

D

S

P

P

I

H

E

P

P

A

I

M

L

F

E
|
E

F

W

D

D

R
x
I

T

T

E
x
H

N

N

P

D

F

L

R

M

Q

T

A

R

V

L

V

A

T

S

T

Y

P

D

I

L

E

K

H

L

D

E

R

N

G

G

V

L

V

V

A

Q

I

P

P

P

D

Q

G

G

P

P

G

G

L

L

G

G

I

F

E

E

I

I

D

D

R

W

E

D

A

F

L

V

A

A

R

A

F

H

A

A

active site: M20 (≠ F19), G53 (= G50), D56 (vs. gap), S143 (≠ T134), K170 (= K164), K172 (= K166), D200 (= D194), N202 (= N196), E226 (= E220), G252 (= G245), E253 (= E246), N254 (≠ T247), Q274 (= Q267), D276 (= D269), H303 (= H296), T304 (≠ V297), F305 (≠ W298), E328 (= E329), I331 (≠ R332), H333 (≠ E334)
binding L-arabinaric acid: K172 (= K166), D200 (= D194), N202 (= N196), E253 (= E246), H303 (= H296), F305 (≠ W298), E328 (= E329)
binding magnesium ion: D200 (= D194), E226 (= E220), E253 (= E246)

3ozmA Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
32% identity, 99% coverage: 1:374/378 of query aligns to 2:373/386 of 3ozmA

query
sites
3ozmA

M

L

K

K

I

I

A

T

A

E

V

V

H

K

T

A

H

H

I

A

L

L

D

S

H

T

V

P

L

I

D

P

T

E

T

R

F
x
M

-

R

-

V

E

E

S
|
S

A

G

S

A

M

G

Y

L

F
x
K

A

L

R

N

R

R

Q

Q

H

M

C

I

L

L

V

V

E

E

I

V

V

R

C

T

D

D

D

E

G

G

T

V

I

T

G

G

W

V

G

G

E

S

C

P

L

S

G
|
G

P

P

-

Y

-
x
D

-

L

A

A

Q

V

P

L

N

K

A

R

A

A

V

I

V

E

K

D

A

V

Y

I

R

G

A

P

A

Q

L

L

I

I

G

G

Q

E

D

D

P

P

L

A

Q

N

T

I

E

N

R

Y

L

L

W

W

L

H

E

K

L

V

Y

F

N

H

R

G

-

E

-

V

L

S

R

R

D

N

Q

L

G

G

Q

H

R

R

G

S

L

V

T

G

V

I

T

A

A

A

L

M

S

S

G

G

I

V

D

D

I

I

A

A

L

L

W

W

D

D

I

L

K

K

G

G

K

R

R

A

F

M

G

N

V

Q

P

P

I

I

S

Y

T

Q

L

L

G

G

R

K

F

F

R

H

D

T

-

R

S

G

V

V

R

R

A

A

Y

Y

A

A

T
x
S

G

S

S

I

F
x
Y

R

W

R

D

A

L

G

T

V

P

D

D

R

Q

V

A

E

A

D

D

N

-

A

-

R

-

E

E

V

L

A

A

G

G

Y

W

R

V

R

E

E

Q

G

G

F

F

H

T

A

A

V

A

K
|
K

I

L

K
|
K

I

V

G

G

F

R

G

A

V

P

D

R

E

K

D

D

L

A

R

A

V

N

I

L

E

R

A

A

V

M

R

R

E

Q

S

R

I

V

G

G

P

A

D

D

M

V

R

E

L

I

M

L

I

V

D
|
D

A

A

N
|
N

H

Q

G

S

Y

L

D

G

Y

R

L

H

E

D

A

A

V

L

E

A

V

M

G

L

R

R

R

I

A

L

A

D

K

E

F

A

G

G

I

C

D

Y

W

W

F

F

E
|
E

E

E

P

P

V

L

L

S

P

I

E

D

H

D

V

I

S

E

A

G

Y

H

R

R

A

I

V

L

R

R

A

A

D

Q

-

G

Q

T

P

P

I

V

P

R

V

I

A

A

G

T

G
|
G

E
|
E

T
x
N

W

L

H

Y

G

T

R

R

Y

N

A

A

M

F

H

N

E

D

P

Y

L

I

A

R

T

N

R

D

A

A

V

I

D

D

I

V

I

L

Q
|
Q

P

A

D
|
D

I

A

C

S

G

R

V

A

G

G

F

I

T

T

E

E

I

A

R

L

R

A

V

I

A

S

D

A

M

S

A

A

L

S

H

A

G

H

V

L

R

A

L

W

V

N

P

P

H
|
H

V
x
T

W
x
F

G

N

T

D

A

I

V

I

C

T

L

V

A

A

S

N

L

L

Q

H

F

L

M

V

A

A

L

-

P

-

N

-

P

-

R

-

D

S

P

P

I

H

E

P

P

A

I

M

L

F

E
|
E

F

W

D

D

R
x
I

T

T

E
x
H

N

N

P

D

F

L

R

M

Q

T

A

R

V

L

V

A

T

S

T

Y

P

D

I

L

E

K

H

L

D

E

R

N

G

G

V

L

V

V

A

Q

I

P

P

P

D

Q

G

G

P

P

G

G

L

L

G

G

I

F

E

E

I

I

D

D

R

W

E

D

A

F

L

V

A

A

R

A

F

H

A

A

active site: M20 (≠ F19), G53 (= G50), D56 (vs. gap), S143 (≠ T134), K170 (= K164), K172 (= K166), D200 (= D194), N202 (= N196), E226 (= E220), G252 (= G245), E253 (= E246), N254 (≠ T247), Q274 (= Q267), D276 (= D269), H303 (= H296), T304 (≠ V297), F305 (≠ W298), E328 (= E329), I331 (≠ R332), H333 (≠ E334)
binding D-xylaric acid: S24 (= S21), K29 (≠ F26), Y146 (≠ F137), K170 (= K164), K172 (= K166), D200 (= D194), N202 (= N196), E253 (= E246), H303 (= H296), F305 (≠ W298), E328 (= E329)
binding magnesium ion: D200 (= D194), E226 (= E220), E253 (= E246)

3op2A Crystal structure of putative mandelate racemase from bordetella bronchiseptica rb50 complexed with 2-oxoglutarate/phosphate
32% identity, 99% coverage: 1:374/378 of query aligns to 2:368/375 of 3op2A

query
sites
3op2A

M

L

K

K

I

I

A

T

A

E

V

V

H

K

T

A

H

H

I

A

L

L

D

S

H

T

V

P

L

I

D

P

T

E

T

R

F
x
M

-

R

-

V

E

E

S

S

A

G

S

A

M

G

Y

L

F

K

A

L

R

N

R

R

Q

Q

H

M

C

I

L

L

V

V

E

E

I

V

V

R

C

T

D

D

D

E

G

G

T

V

I

T

G

G

W

V

G

G

E

S

C

P

L

S

G
|
G

P

P

-

Y

-
x
D

-

L

A

A

Q

V

P

L

N

K

A

R

A

A

V

I

V

E

K

D

A

V

Y

I

R

G

A

P

A

Q

L

L

I

I

G

G

Q

E

D

D

P

P

L

A

Q

N

T

I

E

N

R

Y

L

L

W

W

L

H

E

K

L

V

Y

F

N

H

R

-

L

-

R

-

D

G

Q

E

G

V

Q

S

R

R

G

S

L

V

T

G

V

I

T

A

A

A

L

M

S

S

G

G

I

V

D

D

I

I

A

A

L

L

W

W

D

D

I

L

K

K

G

G

K

R

R

A

F

M

G

N

V

Q

P

P

I

I

S

Y

T

Q

L

L

G

G

R

K

F

F

R

H

D

T

-

R

S

G

V

V

R

R

A

A

Y

Y

A

A

T
x
S

G

S

S

I

F

Y

R

W

R

D

A

L

G

T

V

P

D

D

R

Q

V

A

E

A

D

D

N

-

A

-

R

-

E

E

V

L

A

A

G

G

Y

W

R

V

R

E

E

Q

G

G

F

F

H

T

A

A

V

A

K
|
K

I

L

K
|
K

I

V

G

G

F

R

G

A

V

P

D

R

E

K

D

D

L

A

R

A

V

N

I

L

E

R

A

A

V

M

R

R

E

Q

S

R

I

V

G

G

P

A

D

D

M

V

R

E

L

I

M

L

I

V

D
|
D

A

A

N
|
N

H

Q

G

S

Y

L

D

G

Y

R

L

H

E

D

A

A

V

L

E

A

V

M

G

L

R

R

R

I

A

L

A

D

K

E

F

A

G

G

I

C

D

Y

W

W

F

F

E
|
E

E

E

P

P

V

L

L

S

P

I

E

D

H

D

V

I

S

E

A

G

Y

H

R

R

A

I

V

L

R

R

A

A

D

Q

-

G

Q

T

P

P

I

V

P

R

V

I

A

A

G

T

G
|
G

E
|
E

T
x
N

W

L

H

Y

G

T

R

R

Y

N

A

A

M

F

H

N

E

D

P

Y

L

I

A

R

T

N

R

D

A

A

V

I

D

D

I

V

I

L

Q
|
Q

P

A

D
|
D

I

A

C

S

G

R

V

A

G

G

F

I

T

T

E

E

I

A

R

L

R

A

V

I

A

S

D

A

M

S

A

A

L

S

H

A

G

H

V

L

R

A

L

W

V

N

P

P

H
|
H

V
x
T

W
x
F

G

N

T

D

A

I

V

I

C

T

L

V

A

A

S

N

L

L

Q

H

F

L

M

V

A

A

L

-

P

-

N

-

P

-

R

-

D

S

P

P

I

H

E

P

P

A

I

M

L

F

E
|
E

F

W

D

D

R
x
I

T

T

E
x
H

N

N

P

D

F

L

R

M

Q

T

A

R

V

L

V

A

T

S

T

Y

P

D

I

L

E

K

H

L

D

E

R

N

G

G

V

L

V

V

A

Q

I

P

P

P

D

Q

G

G

P

P

G

G

L

L

G

G

I

F

E

E

I

I

D

D

R

W

E

D

A

F

L

V

A

A

R

A

F

H

A

A

active site: M20 (≠ F19), G53 (= G50), D56 (vs. gap), S138 (≠ T134), K165 (= K164), K167 (= K166), D195 (= D194), N197 (= N196), E221 (= E220), G247 (= G245), E248 (= E246), N249 (≠ T247), Q269 (= Q267), D271 (= D269), H298 (= H296), T299 (≠ V297), F300 (≠ W298), E323 (= E329), I326 (≠ R332), H328 (≠ E334)
binding 2-oxoglutaric acid: K165 (= K164), K167 (= K166), D195 (= D194), E248 (= E246), H298 (= H296), E323 (= E329)
binding magnesium ion: D195 (= D194), E221 (= E220), E248 (= E246)

2ox4C Crystal structure of putative dehydratase from zymomonas mobilis zm4
31% identity, 92% coverage: 33:378/378 of query aligns to 24:393/397 of 2ox4C

query
sites
2ox4C

L

L

V

V

E

K

I

V

V

S

C

T

D

D

D

E

G

G

T

I

I

Y

G

G

W

L

G

G

E

E

C

A

-

G

-

I

-

A

L

Y

G

G

P

V

A

G

Q

G

P

S

N

A

A

A

-

G

V

I

V

L

K

K

A

D

Y

Y

R

A

A

A

A

L

L

L

I

I

G

G

Q

E

D

D

P

P

L

F

Q

N

T

T

E

E

R

A

L

I

W

W

L

E

E

K

L

L

Y

F

N

K

R

K

-

T

L

F

R

W

D

G

Q

Q

G

G

Q

G

R

G

G

T

L

V

T

I

V

F

T

S

A

G

L

I

S

S

G

A

I

F

D

D

I

I

A

A

L

F

W

W

D

D

I

I

K

K

G

G

K

K

R

A

F

L

G

N

V

L

P

P

I

V

S

Y

T

K

L

L

G

G

R

K

F

N

R

R

D

E

S

D

V

L

R

R

A

V

Y

Y

A

A

T

S

G

Q

-

L

-

Q

-

F

-

G

-

W

S

G

F

K

R

E

R

R

A

K

G

S

V

K

D

G

R

R

V

K

E

E

D

E

N

Y

A

A

R

E

E

E

V

A

A

L

G

K

Y

A

R

V

R

A

E

E

G

G

F

Y

H

D

A

A

V

V

K
|
K

I

V

K
x
D

I

V

-

L

-

A

-

H

-

D

-

R

-

N

-

G

-

S

-

R

-

E

-

G

-

V

-

F

-

L

-

E

G

G

F

P

G

L

V

P

D

S

E

E

D

T

L

I

R

K

V

I

-

G

-

V

-

E

-

R

I

V

E

E

A

A

V

I

R

R

E

N

S

A

I

V

G

G

P

P

D

D

M

V

R

D

L

I

M

I

I

V

D
x
E

A

-

N

N

H
|
H

G

G

Y

H

-

T

D

D

Y

L

L

V

E

S

A

A

V

I

E

Q

V

F

G

A

R

K

R

A

A

I

A

E

K

E

F

F

G

N

I

I

D

F

W

F

F

Y

E
|
E

E

E

P

I

V

N

L

T

P

P

E

L

H

N

V

P

S

R

A

L

Y

L

R

K

A

E

V

A

R

K

A

K

D

K

Q

I

P

D

I

I

P

P

V

L

A

A

G

S

G
|
G

E
|
E

T

R

W

I

H

Y

G

S

R

R

Y

W

A

G

M

F

H

L

E

P

P

F

L

L

A

E

T

D

R

R

A

S

V

I

D

D

I

V

I

I

Q
|
Q

P

P

D
|
D

I

L

C

G

G

T

V

C

G

G

F

F

T

T

E

E

I

F

R

K

V

I

A

A

D

D

M

M

A

A

A

H

L

I

H

F

G

E

V

V

R

T

L

V

V

Q

P

A

H
|
H

V

V

W

A

G

G

T

T

A

G

V

V

C

A

L

E

A

A

S

S

L

L

Q

H

F

A

M

E

A

I

A

A

L

-

L

I

P

P

N

N

P

F

P

C

R

I

R

H

D
x
E

P

-

I

-

E

-

P

-

I

-

L

-

E

H

F

H

D

Q

R

K

T

T

E

L

N

L

P

P

F

E

R

Y

Q

E

A

E

V

L

V

C

T

V

T

H

P

N

I

Y

E

Q

H

P

D

V

R

K

G

G

V

R

V

Y

A

K

I

V

P

P

D

E

G

L

P

P

G

G

L

I

G

G

I

Q

E

D

I

I

D

T

R

E

E

K

-

L

-

Y

A

Q

L

I

A

S

R

D

F

Y

A

V

L

S

V

I

E

E

A

A

active site: K165 (= K164), D167 (≠ K166), E214 (≠ D194), H216 (= H197), E240 (= E220), G265 (= G245), E266 (= E246), Q287 (= Q267), D289 (= D269), H316 (= H296), E341 (≠ D322)
binding magnesium ion: E214 (≠ D194), E240 (= E220), E266 (= E246)

3n4eA Crystal structure of mandelate racemase/muconate lactonizing protein from paracoccus denitrificans pd1222
35% identity, 91% coverage: 32:374/378 of query aligns to 19:361/368 of 3n4eA

query
sites
3n4eA

C

A

L

F

V

V

E

R

I

I

V

T

C

A

D

E

D

D

G

G

T

T

I

V

G

G

W

W

G

G

E

E

C

A

-

S

-

P

-

M

L

L

G

G

P

G

A

I

Q

A

P

S

N

L

A

G

A

V

V

V

V

A

K

R

A

D

Y

I

R

A

A

P

A

F

L

L

I

E

G

G

Q

Q

D

E

P

V

L

L

Q

D

T

H

E

A

R

V

L

L

W

L

L

D

E

R

L

M

Y

M

N

H

R

R

L

L

R

V

D

K

Q

L

G

G

Q

P

R

E

G

G

L

I

T

A

V

T

T

A

A

A

L

L

S

A

G

A

I

C

D

D

I

I

A

A

L

L

W

W

D

D

I

L

K

K

G

G

K

K

R

L

F

L

G

G

V

Q

P

P

I

I

S

Y

T

K

L

L

G

G

R

A

F

W

R

R

D

T

S

R

V

L

R

P

A

C

Y

Y

A

S

T
x
S

-

I

-

G

G

G

S

N

F

A

R

A

R

R

A

S

G

-

V

-

D

-

R

-

V

V

E

D

D

E

N

V

A

V

R

R

E

E

V

V

A

A

G

-

Y

R

R

R

R

V

E

E

G

A

F

E

H

Q

A

P

V

A

K

A

I

V

K
|
K

I

I

G
x
R

F

W

G

D

V

G

D

D

E

R

-

T

-

R

-

C

-

D

-
x
V

-

D

-

I

-

P

-

G

D

D

L

I

R

A

V

K

I

A

E

R

A

A

V

V

R

R

E

E

S

L

I

L

G

G

P

P

D

D

M

A

R

V

L

I

M

G

I

F

D
|
D

A

A

N
|
N

H

N

G

G

Y

Y

D

S

Y

V

L

G

E

G

A

A

V

I

E

R

V

V

G

G

R

R

R

A

A

L

A

E

K

D

F

L

G

G

I

Y

D

S

W

W

F

F

E
|
E

E

E

P

P

V

V

L

Q

P

H

E

Y

H

H

V

V

S

G

A

A

Y

M

R

G

A

E

V

V

R

A

A

Q

D

R

Q

L

P

D

I

I

P

T

V

V

A

S

G

A

G
|
G

E
|
E

T

Q

W

T

H

Y

G

T

R

L

Y

Q

A

A

M

L

H

K

E

D

P

-

L

L

A

I

T

L

R

S

A

G

V

V

D

R

I

M

I

V

Q
|
Q

P

P

D
|
D

I

I

C

V

G

K

V

M

G

G

F

I

T

T

E

G

I

M

R

M

R

Q

V

C

A

A

D

A

M

L

A

A

A

H

L

A

H

H

G

G

V

V

R

E

L

F

V

V

P

P

H
|
H

V
x
Q

W
x
T

G

Q

T

P

A
x
G

V

V

C

G

L

H

A

F

A

A

S

N

L

I

Q

H

F

V

M

L

A

S

A

T

L

L

L

M

P

-

N

-

P

-

P

H

R

M

R

T

D

K

P
|
P

I
x
V

E
|
E

P
x
L

I

A

L

D

E

R

F

W

D

D

R
|
R

T

G

E

R

N

P

P

V

F

F

R

R

Q

N

A

-

V

-

T

-

P

P

I

A

E

E

H

P

D

V

R

D

G

G

V

H

V

F

A

A

I

L

P

G

D

D

G

A

P

P

G

G

L

L

G

G

I

I

E

V

I
x
V

D

D

R
x
E

E

D

A

E

L

L

A

A

R

S

F

R

A

A

active site: S124 (≠ T134), K153 (= K166), R155 (≠ G168), V165 (vs. gap), D190 (= D194), N192 (= N196), E216 (= E220), G241 (= G245), E242 (= E246), Q262 (= Q267), D264 (= D269), H291 (= H296), Q292 (≠ V297), T293 (≠ W298), G296 (≠ A301), P315 (= P323), V316 (≠ I324), E317 (= E325), L318 (≠ P326), R324 (= R332)
binding calcium ion: D190 (= D194), E216 (= E220), E242 (= E246), V352 (≠ I365), E354 (≠ R367)

2ggeD Crystal structure of mandelate racemase/muconate lactonizing enzyme from bacillus subtilis complexed with mg++ at 1.8 a
31% identity, 99% coverage: 1:373/378 of query aligns to 3:366/376 of 2ggeD

query
sites
2ggeD

M

V

K

K

I

I

A

V

A

R

V

I

H

E

T

T

H

F

I

P

L

L

D

F

H

H

V

R

L

L

D

E

T

K

T

P

F
x
Y

E

G

S

D

A

A

S

N

M

G

Y

F

F

K

A

R

R

Y

R

R

Q

T

H

C

C

Y

L

L

V

I

E

R

I

I

V

I

C

T

D

E

D

S

G

G

T

I

I

D

G

G

W

W

G

G

E

E

C

C

L

V

G

D

-

W

-

L

P

P

A

A

Q

L

P

-

N

H

A

V

A

G

V

F

V

T

K

K

A

R

Y

I

R

I

A

P

A

F

L

L

I

L

G

G

Q

K

D

Q

P

A

L

G

Q

S

T

R

E

-

R

-

L

-

W

-

L

L

E

S

L

L

Y

V

N

R

R

T

L

I

R

Q

D

K

Q

W

G

H

Q

Q

R

R

G

-

L

-

T

-

V

-

T

-

A

A

L

A

S

S

G

A

I

V

D

S

I

M

A

A

L

L

W

T

D

E

I

I

K

A

G

A

K

K

R

A

F

A

G

D

V

C

P

S

I

V

S

C

T

E

L

L

L

W

G

G

R

R

F

Y

R

R

D

E

S

E

V

I

R

P

A

V

Y

Y

A

A

T
x
S

G

F

S

Q

F

S

R

Y

R

S

A

D

G

S

V

P

D

Q

R

W

V

I

E

S

D

R

N

S

A

V

R

S

E

N

V

V

A

E

G

A

Y

Q

R

L

R

K

E

K

G

G

F

F

H

E

A

Q

V

I

K
|
K

I

V

K
|
K

I

I

G

G

-

G

F

T

G

S

V

F

D

K

E

E

D

D

L

V

R

R

V

H

I

I

E

N

A

A

V

L

R

Q

E

H

S

T

I

A

G

G

P

S

D

S

M

I

R

T

L

M

M

I

I

L

D
|
D

A

A

N
|
N

H

Q

G

S

Y

Y

D

D

Y

A

L

A

E

A

A

A

V

F

E

K

V

W

G

E

R

R

R

Y

A

F

A

S

K

E

F

W

-

T

G

N

I

I

D

G

W

W

F

L

E
|
E

E

E

P

P

V

L

L

P

P

F

E

D

H

Q

V

P

S

Q

A

D

Y

Y

R

A

A

M

V

L

R

R

A

S

D

R

Q

L

P

S

I

V

P

P

V

V

A

A

G

G

G
|
G

E
|
E

T

N

W

M

H

K

G

G

R

P

Y

A

A

Q

M

Y

H

V

E

P

P

L

L

L

A

S

T

Q

R

R

A

C

V

L

D

D

I

I

Q

Q

P

P

D
|
D

I

V

C

M

G

H

V

V

G

N

G

G

F

I

T

D

E

E

I

F

R

R

R

D

V

C

A

L

D

Q

M

L

A

A

A

R

L

Y

H

F

G

G

V

V

R

R

L

A

V

S

P

A

H
|
H

V

A

W

Y

G

D

T

G

A

S

V

L

C

S

L

R

A

L

A

Y

S

A

L

L

Q

-

F

F

M

A

A

Q

A

A

L

C

L

L

P

P

-

P

-

W

N

S

P

K

P

M

R

K

R
x
N

D
|
D

P

H

I

I

E

E

P

P

I

I

L

-

E
|
E

F

W

D

D

R

V

T

M

E

E

N

N

P

P

F

F

R

T

Q

D

A

L

V

V

V

S

T

L

T

Q

P

P

I

-

E

-

H

-

D

S

R

K

G

G

V

M

V

V

A

H

I

I

P

P

D

K

G

G

P

K

G

G

L

I

G

G

I

T

E

E

I

I

D

N

R

M

E

E

A

I

L

V

A

N

R

R

F

Y

active site: Y21 (≠ F19), S128 (≠ T134), K158 (= K164), K160 (= K166), D189 (= D194), N191 (= N196), E216 (= E220), G241 (= G245), E242 (= E246), D265 (= D269), H292 (= H296), N318 (≠ R321), D319 (= D322), E325 (= E329)
binding magnesium ion: E216 (= E220), E242 (= E246), H292 (= H296)

2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
30% identity, 90% coverage: 33:372/378 of query aligns to 22:385/392 of 2o56A

query
sites
2o56A

L

V

V

V

E

K

I

I

V

N

C

T

D

D

D

E

G

G

T

I

I

S

G

G

W

F

G

G

E

E

C

V

-

G

-

L

-

A

-

Y

-

G

L

V

G

G

P

-

A

A

Q

S

P

A

N

G

A

I

A

G

V

M

V

A

K

K

A

D

Y

L

R

S

A

A

A

I

L

I

I

I

G

G

Q

M

D

D

P

P

L

M

Q

N

T

N

E

E

R

A

L

I

W

W

L

E

E

K

L

M

Y

L

N

K

R

K

-

T

L

F

R

W

D

G

Q

Q

G

G

Q

G

R

G

G

G

L

I

T

F

V

S

T

A

A

A

L

M

S

S

G

G

I

I

D

D

I

I

A

A

L

L

W

W

D

D

I

I

K

K

G

G

K

K

R

A

F

W

G

G

V

V

P

P

I

L

S

Y

T

K

L

M

L

L

G

G

R

K

F

S

R

R

D

E

S

K

V

I

R

R

A

T

Y

Y

A

A

T

S

G

Q

S

L

F

Q

R

F

R

G

A

W

G

G

V

D

D

G

R

S

V

D

E

K

D

D

N

M

A

L

R

T

E

E

V

P

A

E

G

Q

Y

Y

R

A

R

Q

-

A

-

L

-

T

-

A

-

V

-

S

E

E

G

G

F

Y

H

D

A

A

V

I

K
|
K

I

V

K
x
D

I

T

-

V

-

A

-
x
M

-

D

-

R

-

H

-

G

-

N

-

W

-

N

-

Q

-

N

-

L

-

N

G

G

F

P

G
x
L

V

T

D

D

E

K

D

I

L

L

R

R

V

L

-

G

-

Y

-

D

-

R

I

M

E

A

A

A

V

I

R

R

E

D

S

A

I

V

G

G

P

P

D

D

M

V

R

D

L

I

M

I

I

A

D
x
E

A

M

N
x
H

H

A

G

F

Y

T

D

D

Y

T

L

T

E

S

A

A

V

I

E

Q

V

F

G

G

R

R

R

M

A

I

A

E

K

E

F

L

G

G

I

I

D

F

W

Y

F

Y

E
|
E

E

E

P

P

V

V

L

M

P

P

E

L

H

N

V

P

S

A

A

Q

Y

M

R

K

A

Q

V

V

R

A

A

D

D

K

Q

V

P

N

I

I

P

P

V

L

A

A

G

A

G
|
G

E
|
E

T

R

W

I

H

Y

G

W

R

R

Y

W

A

G

M

Y

H

R

E

P

P

F

L

L

A

E

T

N

R

G

A

S

V

L

D

S

I

V

I

I

Q
|
Q

P

P

D
|
D

I

I

C

C

G

T

V

C

G

G

F

I

T

T

E

E

I

V

R

K

V

I

A

C

D

D

M

M

A

A

A

H

L

V

H

Y

G

D

V

K

R

T

L

V

V

Q

P

I

H
|
H

V

V

W

C

G

G

T

G

A

P

V

I

C

S

L

T

A

A

A

V

S

A

L

L

Q

H

F

M

M

E

A

T

A

A

L

I

L

-

P

-

N

-

P

-

R

-

D

-

P

P

I

N

E

F

P

V

I

I

L

H

E
|
E

F

L

D

H

R

R

T

Y

E
x
A

-

L

N

E

P

P

F

N

R

T

Q

Q

A

T

V

C

V

K

T

Y

T

N

P

Y

I

L

E

P

H

K

D

N

R

-

G

G

V

M

V

Y

A

E

I

V

P

P

D

E

G

L

P

P

G

G

L

I

G

G

I

Q

E

E

I

L

D

T

R

E

E

E

A

T

L

M

A

K

R

K

active site: K165 (= K164), D167 (≠ K166), M171 (vs. gap), L186 (≠ G170), E214 (≠ D194), H216 (≠ N196), E240 (= E220), G265 (= G245), E266 (= E246), Q287 (= Q267), D289 (= D269), H316 (= H296), E341 (= E329), A346 (≠ E334)
binding magnesium ion: E214 (≠ D194), E240 (= E220), E266 (= E246)

2pp1A Crystal structure of l-talarate/galactarate dehydratase from salmonella typhimurium lt2 liganded with mg and l-lyxarohydroxamate (see paper)
34% identity, 80% coverage: 66:369/378 of query aligns to 97:381/395 of 2pp1A

query
sites
2pp1A

L

L

I

L

G

G

Q

E

D

D

P

P

L

N

Q

D

T

I

E

D

R

K

L

I

W

Y

L

T

E

K

L

L

Y

L

N

W

R

A

L

G

R

A

D

S

Q

V

G

G

Q

R

R

S

G

G

L

M

T

A

V

V

T

Q

A

A

L

I

S

S

G

P

I

I

D

D

I

I

A

A

L

L

W

W

D

D

I

M

K

K

G

A

K

K

R

R

F

A

G

G

V

L

P

P

I

L

S

A

T

K

L

L

G

G

G

A

R

H

F

-

R

R

D

D

S

S

V

V

R

Q

A

C

Y

Y

A

N

T

T

-

S

G

G

S

G

F
|
F

R

L

R

H

A

T

G

P

V

L

D

D

R

Q

V

V

E

L

D

K

N

N

A

-

R

-

E

-

V

V

A

V

G

I

Y

S

R

R

R

E

E

N

G

G

F

I

H

G

A

G

V

I

K
|
K

I

L

K
|
K

I

V

G

G

F

Q

-

P

G

N

V

C

D

A

E

E

D

D

L

I

R

R

V

R

I

L

E

T

A

A

V

V

R

R

E

E

S

A

I

L

G

G

P

D

D

E

M

F

R

P

L

L

M

M

I

V

D
|
D

A

A

N
|
N

H

Q

G

Q

Y

W

D

D

Y

R

L

E

E

T

A

A

V

I

E

R

V

M

G

G

R

R

R

K

A

M

A

E

K

Q

F

F

G

N

I

L

D

I

W

W

F

I

E
|
E

E

E

P

P

V

L

L

D

P

A

E

Y

H

D

V

I

S

E

A

G

Y

H

R

A

A

Q

V

L

R

A

A

A

D

A

Q

L

P

D

I

T

P

P

V

I

A

A

G

T

G
|
G

E
|
E

T

M

W

L

H

T

G

S

R

F

Y

R

A

E

M

H

H

E

E

Q

P

L

L

I

A

L

T

G

R

N

A

A

V

S

D

D

I

F

I

V

Q

Q

P

P

D
|
D

I

A

C

P

G

R

V

V

G

G

F

I

T

S

E

P

I

F

R

L

R

K

V

I

A

M

D

D

M

L

A

A

L

K

H

H

G

G

V

R

R

K

L

L

V

A

P

P

H
|
H

V

-

W

-

G

-

T

-

A

-

V

F

C

A

L

M

A

E

A

V

S

H

L

L

Q

H

F

L

M

S

A

A

L

Y

L

-

P

-

N

-

P

-

R

-

D

-

P

P

I

L

E

E

P

P

I

W

L

L

E
|
E

F

H

D

F

R

E

T

W

E

L

N

N

P

P

F

-

R

-

Q

-

A

-

V

L

V

F

T

N

T

E

P

Q

I

L

E

E

H

L

D

R

R

D

G

G

V

R

V

M

A

W

I

I

P

S

D

D

G

R

P

H

G

G

L

L

G

G

I

F

E

T

I

L

D

S

R

E

E

Q

A

A

active site: K192 (= K164), K194 (= K166), D223 (= D194), N225 (= N196), E249 (= E220), G274 (= G245), E275 (= E246), D298 (= D269), H325 (= H296), E345 (= E329)
binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: F168 (= F137), K194 (= K166), E275 (= E246), H325 (= H296), E345 (= E329)
binding magnesium ion: D223 (= D194), E249 (= E220), E275 (= E246)

Sites not aligning to the query:

active site: 45, 78
binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: 43, 45, 79

Q8ZL58 L-talarate/galactarate dehydratase; TalrD/GalrD; StTGD; EC 4.2.1.156; EC 4.2.1.42 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 80% coverage: 66:369/378 of query aligns to 100:384/398 of Q8ZL58

query
sites
Q8ZL58

L

L

I

L

G

G

Q

E

D

D

P

P

L

N

Q

D

T

I

E

D

R

K

L

I

W

Y

L

T

E

K

L

L

Y

L

N

W

R

A

L

G

R

A

D

S

Q

V

G

G

Q

R

R

S

G

G

L

M

T

A

V

V

T

Q

A

A

L

I

S

S

G

P

I

I

D

D

I

I

A

A

L

L

W

W

D

D

I

M

K

K

G

A

K

K

R

R

F

A

G

G

V

L

P

P

I

L

S

A

T

K

L

L

G

G

G

A

R

H

F

-

R

R

D

D

S

S

V

V

R

Q

A

C

Y

Y

A

N

T

T

-

S

G

G

S

G

F

F

R

L

R

H

A

T

G

P

V

L

D

D

R

Q

V

V

E

L

D

K

N

N

A

-

R

-

E

-

V

V

A

V

G

I

Y

S

R

R

R

E

E

N

G

G

F

I

H

G

A

G

V

I

K
|
K

I

L

K
|
K

I

V

G

G

F

Q

-

P

G

N

V

C

D

A

E

E

D

D

L

I

R

R

V

R

I

L

E

T

A

A

V

V

R

R

E

E

S

A

I

L

G

G

P

D

D

E

M

F

R

P

L

L

M

M

I

V

D
|
D

A

A

N
|
N

H

Q

G

Q

Y

W

D

D

Y

R

L

E

E

T

A

A

V

I

E

R

V

M

G

G

R

R

R

K

A

M

A

E

K

Q

F

F

G

N

I

L

D

I

W

W

F

I

E
|
E

E

E

P

P

V

L

L

D

P

A

E

Y

H

D

V

I

S

E

A

G

Y

H

R

A

A

Q

V

L

R

A

A

A

D

A

Q

L

P

D

I

T

P

P

V

I

A

A

G

T

G

G

E
|
E

T

M

W

L

H

T

G

S

R

F

Y

R

A

E

M

H

H

E

E

Q

P

L

L

I

A

L

T

G

R

N

A

A

V

S

D

D

I

F

I

V

Q

Q

P

P

D

D

I

A

C

P

G

R

V

V

G

G

F

I

T

S

E

P

I

F

R

L

R

K

V

I

A

M

D

D

M

L

A

A

L

K

H

H

G

G

V

R

R

K

L

L

V

A

P

P

H
|
H

V

-

W

-

G

-

T

-

A

-

V

F

C

A

L

M

A

E

A

V

S

H

L

L

Q

H

F

L

M

S

A

A

L

Y

L

-

P

-

N

-

P

-

R

-

D

-

P

P

I

L

E

E

P

P

I

W

L

L

E
|
E

F

H

D

F

R

E

T

W

E

L

N

N

P

P

F

-

R

-

Q

-

A

-

V

L

V

F

T

N

T

E

P

Q

I

L

E

E

H

L

D

R

R

D

G

G

V

R

V

M

A

W

I

I

P

S

D

D

G

R

P

H

G

G

L

L

G

G

I

F

E

T

I

L

D

S

R

E

E

Q

A

A

K195 (= K164) binding substrate
K197 (= K166) active site, Proton acceptor; mutation to A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
D226 (= D194) binding Mg(2+)
N228 (= N196) binding substrate
E252 (= E220) binding Mg(2+)
E278 (= E246) binding Mg(2+)
H328 (= H296) active site, Proton donor/acceptor; mutation H->N,A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
E348 (= E329) binding substrate

Sites not aligning to the query:

46:48 binding substrate
82:83 binding substrate

5xd8B Crystal structure analysis of 3,6-anhydro-l-galactonate cycloisomerase (see paper)
31% identity, 82% coverage: 63:373/378 of query aligns to 70:360/367 of 5xd8B

query
sites
5xd8B

R

Q

A

P

A

A

L

L

I

I

G

G

Q

K

D

D

P

A

L

T

Q

Q

T

I

E

E

R

E

L

I

W

Y

L

D

E

F

L

M

Y

E

N

W

R

H

L

I

R

H

D

Y

Q

V

G

G

Q

R

R

G

G

G

L

I

T

S

V

T

T

F

A

A

L

M

S

S

G

A

I

V

D

D

I

I

A

A

L

L

W

W

D

D

I

L

K

K

G

G

K

K

R

R

F

E

G

G

V

L

P

P

I

L

S

W

T

K

L

M

L

A

G

G

R

K

F

-

R

N

D

N

S

T

V

C

R

K

A

A

Y

Y

A

C

T
x
G

G

G

S

I

F

D

R

L

R

Q

A

F

G

P

V

L

D

E

R

K

V

L

E

L

D

N

N

N

A

-

R

-

E

-

V

I

A

C

G

G

Y

Y

R

L

R

E

E

S

G

G

F

F

H

N

A

A

V

V

K
|
K

I

I

K
|
K

I

I

G

G

-

R

F

E

G

N

V

M

D

Q

E

E

D

D

L

I

R

D

V

R

I

I

E

K

A

A

V

V

R

R

E

E

S

L

I

I

G

G

P

P

D

D

M

I

R

T

L

F

M

M

I

I

D
|
D

A

A

N
|
N

H

Y

G

S

Y

L

D

T

Y

V

L

E

E

Q

A

A

V

I

E

K

V

L

G

S

R

K

R

A

A

V

A

E

K

Q

F

Y

G

D

I

I

D

T

W

W

F

F

E
|
E

E

E

P

P

V

T

L

L

P

P

E

D

H

D

V

Y

S

K

A

G

Y

F

R

A

A

E

V

I

R

A

A

D

D

N

Q

T

P

A

I

I

P

P

V

L

A

A

G

M

G
|
G

E
|
E

T

N

W

L

H

H

G

T

R

I

Y

H

A

E

M

F

H

G

E

Y

P

A

L

M

A

D

T

Q

R

A

A

K

V

L

D

G

I

Y

I

C

Q
|
Q

P

P

D
|
D

I

A

C

S

G

N

V

C

G

G

F

I

T

T

E

G

I

W

R

L

R

K

V

A

A

A

D

D

M

L

A

I

A

T

L

E

H

H

G

N

V

I

R

P

L

V

V

C

P

T

H
|
H

V

-

W

-

G

-

T

-

A

-

V

-

C

-

L

-

A

-

S
x
G

L
x
M

Q

Q

F

E

M

L

A

H

A

V

L

S

L

L

P

V

N

S

P

A

P

F

R

D

R

T

D

G

P
x
W

I

L

E
|
E

-

V

-

H

-

S

-

F

P
|
P

I

I

L

D

E

E

F

Y

D

-

R

-

T

T

E

K

N

R

P

P

F

L

R

-

Q

-

A

-

V

-

T

-

P

V

I

V

E

E

H

N

D

F

R

R

G

A

V

V

V

A

A

S

I

-

P

-

D

N

G

E

P

P

G

G

L

I

G

G

I

V

E

E

I

F

D

D

R

W

E

D

A

K

L

I

A

A

R

Q

F

Y

active site: G140 (≠ T134), K167 (= K164), K169 (= K166), D198 (= D194), N200 (= N196), E224 (= E220), G249 (= G245), E250 (= E246), Q271 (= Q267), D273 (= D269), H300 (= H296), G301 (≠ S307), M302 (≠ L308), W317 (≠ P323), E319 (= E325), P324 (= P326)
binding magnesium ion: D198 (= D194), E224 (= E220), E250 (= E246)

Sites not aligning to the query:

active site: 22, 53

2pp3A Crystal structure of l-talarate/galactarate dehydratase mutant k197a liganded with mg and l-glucarate (see paper)
33% identity, 80% coverage: 66:369/378 of query aligns to 97:381/395 of 2pp3A

query
sites
2pp3A

L

L

I

L

G

G

Q

E

D

D

P

P

L

N

Q

D

T

I

E

D

R

K

L

I

W

Y

L

T

E

K

L

L

Y

L

N

W

R

A

L

G

R

A

D

S

Q

V

G

G

Q

R

R

S

G

G

L

M

T

A

V

V

T

Q

A

A

L

I

S

S

G

P

I

I

D

D

I

I

A

A

L

L

W

W

D

D

I

M

K

K

G

A

K

K

R

R

F

A

G

G

V

L

P

P

I

L

S

A

T

K

L

L

G

G

G

A

R

H

F

-

R

R

D

D

S

S

V

V

R

Q

A

C

Y

Y

A

N

T

T

-

S

G

G

S

G

F

F

R

L

R

H

A

T

G

P

V

L

D

D

R

Q

V

V

E

L

D

K

N

N

A

-

R

-

E

-

V

V

A

V

G

I

Y

S

R

R

R

E

E

N

G

G

F

I

H

G

A

G

V

I

K
|
K

I

L

K
x
A

I

V

G

G

F

Q

-

P

G

N

V

C

D

A

E

E

D

D

L

I

R

R

V

R

I

L

E

T

A

A

V

V

R

R

E

E

S

A

I

L

G

G

P

D

D

E

M

F

R

P

L

L

M

M

I

V

D
|
D

A

A

N
|
N

H

Q

G

Q

Y

W

D

D

Y

R

L

E

E

T

A

A

V

I

E

R

V

M

G

G

R

R

R

K

A

M

A

E

K

Q

F

F

G

N

I

L

D

I

W

W

F

I

E
|
E

E

E

P

P

V

L

L

D

P

A

E

Y

H

D

V

I

S

E

A

G

Y

H

R

A

A

Q

V

L

R

A

A

A

D

A

Q

L

P

D

I

T

P

P

V

I

A

A

G

T

G
|
G

E
|
E

T

M

W

L

H

T

G

S

R

F

Y

R

A

E

M

H

H

E

E

Q

P

L

L

I

A

L

T

G

R

N

A

A

V

S

D

D

I

F

I

V

Q

Q

P

P

D
|
D

I

A

C

P

G

R

V

V

G

G

F

I

T

S

E

P

I

F

R

L

R

K

V

I

A

M

D

D

M

L

A

A

L

K

H

H

G

G

V

R

R

K

L

L

V

A

P

P

H
|
H

V

-

W

-

G

-

T

-

A

-

V

F

C

A

L

M

A

E

A

V

S

H

L

L

Q

H

F

L

M

S

A

A

L

Y

L

-

P

-

N

-

P

-

R

-

D

-

P

P

I

L

E

E

P

P

I

W

L

L

E
|
E

F

H

D
x
F

R

E

T

W

E

L

N

N

P

P

F

-

R

-

Q

-

A

-

V

L

V

F

T

N

T

E

P

Q

I

L

E

E

H

L

D

R

R

D

G

G

V

R

V

M

A

W

I

I

P

S

D

D

G

R

P

H

G

G

L

L

G

G

I

F

E

T

I

L

D

S

R

E

E

Q

A

A

active site: K192 (= K164), A194 (≠ K166), D223 (= D194), N225 (= N196), E249 (= E220), G274 (= G245), E275 (= E246), D298 (= D269), H325 (= H296), E345 (= E329)
binding l-glucaric acid: K192 (= K164), D223 (= D194), N225 (= N196), E275 (= E246), H325 (= H296), E345 (= E329), F347 (≠ D331)
binding magnesium ion: D223 (= D194), E249 (= E220), E275 (= E246)

Sites not aligning to the query:

active site: 45, 78
binding l-glucaric acid: 43, 79

3stpA Crystal structure of a putative galactonate dehydratase
29% identity, 93% coverage: 26:377/378 of query aligns to 42:390/390 of 3stpA

query
sites
3stpA

F

F

A

R

R

F

R

H

Q

Q

H

W

C

L

L

T

V

C

E

E

I

V

V

E

C

T

D

E

D

D

G

G

T

T

I

I

G

G

W

I

G

G

E

N

C

A

-

A

L
|
L

G

A

P

P

A
x
S

Q

V

P

V

N

K

A

K

A

V

V

I

V

D

K

D

A

W

Y

Y

R

A

A

P

A

L

L

V

I

I

G

G

Q

E

D

D

P

P

L

F

Q

D

T

Y

E

A

R

Y

L

I

W

W

L

E

E

K

L

M

Y

Y

N

R

R

R

L

S

R

H

D

A

Q

W

G

G

Q

R

R

K

G

G

L

I

T

G

V

M

T

T

A

A

L

I

S

S

G

A

I

I

D

D

I

I

A

A

L

I

W

W

D

D

I

L

K

M

G

G

K

K

R

L

F

V

G

G

V

K

P

P

I

V

S

F

T

K

L

L

G

G

R

R

F

T

R

K

D

D

S

R

V

I

R

P

A

V

Y

Y

A

-

T

-

G

-

S

-

F

Y

R
x
S

R

K

A

L

G

Y

V

A

D

G

R

S

V

I

E

E

D

A

N

M

A

Q

R

K

E

E

V

A

A

E

G

E

Y

A

R

M

R

K

E

G

G

G

F

Y

H

K

A

A

V

F

K
|
K

I

S

K
x
R

I

F

G

G

F

Y

G

G

-

P

-

K

-

D

-

G

-

M

-
x
P

-

G

V

M

D

R

E

E

D

N

L

L

R

K

V

R

I

V

E

E

A

A

V

V

R

R

E

E

S

V

I

I

G

G

P

Y

D

D

M

N

R

D

L

L

M

M

I

L

D
x
E

A

C

N
x
Y

H

M

G

G

Y

W

D

N

Y

L

L

D

E

Y

A

A

V

K

E

R

V

M

G

L

R

P

R

K

A

L

A

A

K

P

F

Y

G

E

I

P

D

R

W

W

F

L

E
|
E

E

E

P

P

V

V

L

I

P

A

E

D

H

D

V

V

S

A

A

G

Y

Y

R

A

A

E

V

L

R

N

A

A

D

M

Q

N

P

I

I

V

P

P

V

I

A

S

G

G

G
|
G

E
|
E

T
x
H

W

E

H

F

G

S

R

V

Y

I

A

G

M

C

H

A

E

E

P

L

L

I

A

N

T

R

R

K

A

A

V

V

D

S

I

V

I

L

Q
|
Q

P

Y

D
|
D

I

T

C

N

G

R

V

V

G

G

F

I

T

T

E

A

I

A

R

Q

R

K

V

I

A

N

D

A

M

I

A

A

A

E

L

A

H

A

G

Q

V
x
I

R

P

L

V

V

I

P

P

H
|
H

V

-

W

-

G

-

T

-

A

-

V

-

C

-

L

-

A

A

A

G

S

Q

L

M

Q

H

F

N

M

Y

A

H

A

L

L

T

L

M

P

A

N

N

P

-

R

T

R

N

D

C

P

P

I

I

E

S

-
x
E

-

Y

-

F

P

P

I

V

L
x
F

E

D

F

V

D

E

R

V

T

G

E

N

N

E

P

L

F

F

R

Y

Q

Y

A

I

V

F

V

E

T

G

T

D

P

P

I

E

E

A

H

V

D

D

R

G

G

Y

V

L

V

Q

A

L

I

D

P

D

D

D

G

L

P

P

G

G

L

L

G

G

I

I

E

A

I

I

D

S

R

D

E

K

A

H

L

L

A

Q

R

H

F

F

A

D

L

I

V

T

E

E

active site: L66 (= L49), S69 (≠ A52), S151 (≠ R138), K177 (= K164), R179 (≠ K166), P189 (vs. gap), E214 (≠ D194), Y216 (≠ N196), E240 (= E220), G265 (= G245), E266 (= E246), H267 (≠ T247), Q287 (= Q267), D289 (= D269), I311 (≠ V291), H316 (= H296), E336 (vs. gap), F341 (≠ L328)
binding magnesium ion: E214 (≠ D194), E240 (= E220), E266 (= E246)

3sqsA Crystal structure of a putative mandelate racemase/muconate lactonizing protein from dinoroseobacter shibae dfl 12
28% identity, 93% coverage: 26:377/378 of query aligns to 38:386/386 of 3sqsA

query
sites
3sqsA

F

F

A

R

R

F

R

H

Q

Q

H

W

C

L

L

T

V

C

E

E

I

V

V

E

C

T

D

E

D

D

G

G

T

T

I

I

G

G

W

I

G

G

E

N

C

A

-

A

L
|
L

G

A

P

P

A
x
N

Q

V

P

V

N

K

A

Q

A

A

V

I

V

D

K

E

A

W

Y

Y

R

A

A

P

A

L

L

V

I

I

G

G

Q

E

D

D

P

P

L

F

Q

D

T

Y

E

A

R

Y

L

L

W

W

L

E

E

K

L

M

Y

Y

N

R

R

R

L

T

R

H

D

A

Q

W

G

G

Q

R

R

K

G

G

L

I

T

G

V

M

T

T

A

A

L

I

S

S

G

A

I

I

D

D

I

I

A

A

L

I

W

W

D

D

I

L

K

M

G

G

K

K

R

L

F

V

G

G

V

K

P

P

I

V

S

F

T

K

L

L

G

G

R

R

F

T

R

K

D

E

S

K

V

I

R

P

A

V

Y

Y

A

-

T

-

G

-

S

-

F

Y

R
x
S

R

K

A

L

G

Y

V

A

D

D

R

S

V

I

E

P

D

A

N

M

A

Q

R

A

E

E

V

A

A

E

G

E

Y

A

R

Q

R

K

E

H

G

G

F

Y

H

Q

A

G

V

Y

K
|
K

I

T

K
x
R

I

F

G
|
G

F

Y

-
x
G

-

P

-
x
K

-

D

-

G

-

P

-
x
A

G

G

V

M

D

R

E

E

D

N

L

L

R

K

V

R

I

V

E

E

A

A

V

L

R

R

E

E

S

V

I

L

G

G

P

Y

D

D

M

V

R

D

L

L

M

M

I

L

D
x
E

A

C

N
x
Y

H

M

G

G

Y

W

D

N

Y

L

L

D

E

Y

A

T

V

K

E

R

V

M

G

L

R

P

R

K

A

L

A

E

K

R

F

F

G

E

I

P

D

R

W

W

F

L

E
|
E

E

E

P

P

V

V

L

I

P

A

E

D

H

D

V

V

S

A

A

G

Y

Y

R

A

A

E

V

L

R

N

A

A

D

M

Q

G

P

I

I

V

P

P

V

I

A

S

G

G

G
|
G

E
|
E

T
x
H

W

E

H

F

G

S

R

V

Y

M

A

G

M

C

H

A

E

E

P

L

L

I

A

N

T

R

R

K

A

A

V

V

D

S

I

V

I

L

Q
|
Q

P

Y

D
|
D

I

T

C

N

G

R

V

V

G

G

F

I

T

T

E

A

I

A

R

Q

R

K

V

I

A

N

D

A

M

I

A

A

A

E

L

A

H

A

G

Q

V
x
I

R

I

L

V

V

I

P

P

H
|
H

V

A

W

-

G
|
G

T

Q

A

M

V

H

C

N

L

Y

A

H

A

L

S

T

L

M

Q

A

F

N

M

M

A

-

L

-

P

-

N

N

P

C

P

P

R

I

R

S

D
x
E

P

Y

I

F

E

-

P

P

I

V

L
x
F

E

D

F

V

D

E

R

V

T

G

E

N

N

E

P

L

F

F

R
x
Y

Q

Y

A

I

V
x
F

V
x
D

T

G

T

D

P

P

I

E

E

A

H

V

D

D

R

G

G

Y

V

L

V

Q

A

L

I

D

P

D

D

D

G

T

P

P

G

G

L

L

G

G

I

I

E

T

I

I

D

S

R

D

E

A

A

H

L

L

A

K

R

H

F

F

A

E

L

I

V

T

E

E

active site: L62 (= L49), N65 (≠ A52), S147 (≠ R138), K173 (= K164), R175 (≠ K166), G177 (= G168), G179 (vs. gap), K181 (vs. gap), A185 (vs. gap), E210 (≠ D194), Y212 (≠ N196), E236 (= E220), G261 (= G245), E262 (= E246), H263 (≠ T247), Q283 (= Q267), D285 (= D269), I307 (≠ V291), H312 (= H296), G314 (= G299), E332 (≠ D322), F337 (≠ L328)
binding magnesium ion: E210 (≠ D194), E236 (= E220), E262 (= E246), Y347 (≠ R338), F350 (≠ V341), D351 (≠ V342)

3ck5A Crystal structure of a racemase from streptomyces coelicolor a3(2) with bound magnesium
31% identity, 90% coverage: 34:375/378 of query aligns to 34:357/357 of 3ck5A

query
sites
3ck5A

V

V

E

R

I

I

V

E

C

D

D

S

D

D

G

G

T

A

I

T

G

G

W

L

G

G

E

Y

C

T

L

Y

G
x
T

P

V

A

N

Q

H

P

G

N

G

A

A

V

V

V

A

-

T

-

M

-

V

-

D

K

K

A

D

Y

L

R

R

A

G

A

C

L

L

I

L

G

G

Q

A

D

D

P

A

L

E

Q

Q

T

I

E

E

R

K

L

I

W

W

L

Q

E

S

L

M

Y

W

N

W

R

R

L

L

R

H

D

Y

Q

A

G

G

Q

R

R

G

G

G

L

H

T

A

V

T

T

S

A

A

L

I

S

S

G

A

I

V

D

D

I

I

A

A

L

L

W

W

D

D

I

L

K

K

G

G

K

I

R

R

F

A

G

R

V

T

P

P

I

L

S

W

T

K

L

L

L

F

G

G

R

-

F

Y

R

D

D

P

S

V

V

V

R

P

A

V

Y

Y

A

A

T
x
G

G

G

S

I

F

D

R

L

R

E

A

L

G

P

V

V

D

A

R

D

V

L

E

K

D

T

N

Q

A

A

-

D

R

R

E

F

V

L

A

A

G

G

Y

-

R

-

E

-

G

G

F

F

H

R

A

A

V

I

K
|
K

I

M

K
|
K

I

V

G

G

F

R

-

P

G

D

V

L

D

K

E

E

D

D

L

V

R

D

V

R

I

V

E

S

A

A

V

L

R

R

E

E

S

H

I

L

G

G

P

D

D

S

M

F

R

P

L

L

M

M

I

V

D
|
D

A

A

N
|
N

H

M

G

K

Y

W

D

T

Y

V

L

D

E

G

A

A

V

I

E

R

V

A

G

A

R

R

R

A

A

L

A

A

K

P

F

F

G

D

I

L

D

H

W

W

F
x
I

E
|
E

E

E

P

P

V

T

L

I

P

P

E

D

H

D

V

L

S

V

A

G

Y

N

R

A

A

R

V

I

R

V

A

R

D

E

Q

S

P

G

I

H

P

T

V
x
I

A

A

G

G

G
|
G

E
|
E

T

N

W

L

H

H

G

T

R

L

Y

Y

A

D

M

F

H

H

E

N

P

A

L

V

A

R

T

A

R

G

A

S

V

L

D

T

I

L

I

P

Q
x
E

P

P

D
|
D

I

V

C

S

G

N

V

I

G

G

F

Y

T

T

E

T

I

F

R

R

R

K

V

V

A

A

D

A

M

L

A

A

A

E

L

A

H

N

G

N

V

M

R

L

L

L

V

T

P

S

H
|
H

V

-

W

-

G

-

T

-

A

-

V

-

C

-

L

-

A

-

A
x
G

S
x
V

L

H

Q

D

F

L

M

T

A

V

A

H

L

A

L

L

P

A

N

S

P

V

P

P

R

H

R

R

D

T

P
x
Y

I

M

E
|
E

P

A

I

H

L

L

E

-

F

-

D

-

R

-

T

-

E

-

N

-

P

-

F

-

R

-

Q

H

A

A

V

Y

V

M

T

A

T

E

P

P

I

M

E

A

H

V

D

T

R

D

G

G

V

C

V

V

A

S

I

A

P

P

D

D

G

R

P

P

G

G

L

H

G

G

I

V

E

V

I

L

D

D

R

F

E

E

A

R

L

L

A

G

R

R

F

L

A

A

L

V

active site: T50 (≠ G50), G137 (≠ T134), K164 (= K164), K166 (= K166), D195 (= D194), N197 (= N196), I220 (≠ F219), E221 (= E220), I243 (≠ V242), G246 (= G245), E247 (= E246), E268 (≠ Q267), D270 (= D269), H297 (= H296), G298 (≠ A306), V299 (≠ S307), Y315 (≠ P323), E317 (= E325)
binding magnesium ion: D195 (= D194), E221 (= E220), E247 (= E246)

Sites not aligning to the query:

active site: 19

4mmwA Crystal structure of d-glucarate dehydratase from agrobacterium tumefaciens complexed with magnesium, l-xylarohydroxamate and l- lyxarohydroxamate
33% identity, 82% coverage: 58:366/378 of query aligns to 62:372/381 of 4mmwA

query
sites
4mmwA

V

I

V

E

K

K

A

F

Y

V

R

K

A

K

A

V

L

L

I

I

G

G

Q

E

D

D

P

H

L

R

Q

D

T

R

E

E

R

R

L

L

W

W

L

Q

E

D

L

L

Y

A

N

H

R

W

L

Q

R

R

D

G

Q

S

G

A

Q

A

R

Q

G

-

L

L

T

T

V

D

T

R

A

T

L

L

S

A

G

V

I

V

D

D

I

C

A

A

L

L

W

W

D

D

I

L

K

A

G

G

K

R

R

S

F

L

G

G

V

Q

P

P

I

V

S

Y

T

K

L

L

L

I

G

G

R

-

F

Y

R

R

D

D

S

K

V

V

R

L

A

A

Y

Y

A

G

T
x
S

-

I

-

M

-
x
C

G

G

S

D

F

E

R

L

R

E

A

G

G

G

V

L

D

A

R

T

V

P

E

E

D

D

N

Y

A

G

R

R

E

F

V

A

A

E

G

T

Y

L

R

V

R

K

E

R

G

G

F

Y

H

K

A

G

V

I

K
|
K

I

L

K
x
H

I

T

G

W

F

M

-

P

-

V

-

S

-

W

-

A

-
x
P

G

D

V

V

D

K

E

M

D

D

L

L

R

K

V

A

I

C

E

A

A

A

V

V

R

R

E

E

S

A

I

V

G

G

P

P

D

D

M

I

R

R

L

L

M

M

I

I

D
|
D

A

A

N
x
F

H
|
H

G

W

Y

Y

D

S

Y

R

L

T

E

D

A

A

V

L

E

A

V

L

G

G

R

R

R

G

A

L

A

E

K

K

F

L

G

G

I

F

D

D

W

W

F

I

E
|
E

E

E

P

P

V

M

L

D

P

E

E

Q

H

S

V

L

S

S

A

S

Y

Y

R

K

A

W

V

L

R
x
S

A

D

D

N

Q
x
L

P

D

I

I

P

P

V

V

A

V

G

G

G
x
P

E
|
E

T

S

W

A

H

A

G

G

R

K

Y

H

A

W

M

H

H

R

-

A

E

E

P

W

L

I

A

K

T

A

R

G

A

A

V

C

D

D

I

I

I

L

Q
x
R

P

T

D
x
G

I

V

C

N

G

D

V

V

G

G

F

I

T

T

E

P

I

A

R

L

R

K

V

T

A

M

D

H

M

L

A

A

A

E

L

A

H

F

G

G

V

M

R

E

L

C

V

E

P

V

H
|
H

V

-

W

-

G
|
G

T
x
N

A

T

V

-

C

-

L

-

A

A

A

M

S

N

L

L

Q

H

F

V

M

V

A

A

L

T

L

K

P

N

-

C

N

R

P

W

P

Y

R
x
E

R

R

D
x
G

P

L

I

L

E

H

P

P

I

F

L

L

E
|
E

F

Y

D

D

R

D

T

G

E

H

N

D

P

Y

F

L

R

K

Q

S

A

-

V

-

V

L

T

S

T

D

P

P

I

M

E

D

H

R

D

D

R

-

G

G

V

F

V

V

A

H

I

V

P

P

D

D

G

R

P

P

G

G

L

L

G

G

I

E

E

D

I

I

D

D

active site: S136 (≠ T134), K169 (= K164), H171 (≠ K166), P181 (vs. gap), D206 (= D194), F208 (≠ N196), E232 (= E220), P257 (≠ G245), E258 (= E246), R280 (≠ Q267), G282 (≠ D269), H309 (= H296), G310 (= G299), N311 (≠ T300), E329 (≠ R320), G331 (≠ D322), E338 (= E329)
binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: C139 (vs. gap), K169 (= K164), H171 (≠ K166), D206 (= D194), H209 (= H197), E258 (= E246), H309 (= H296), E329 (≠ R320)
binding magnesium ion: D206 (= D194), E232 (= E220), S247 (≠ R235), L250 (≠ Q238), E258 (= E246)
binding xylarohydroxamate: C139 (vs. gap), K169 (= K164), H171 (≠ K166), D206 (= D194), H209 (= H197), E258 (= E246), H309 (= H296), E329 (≠ R320)

Sites not aligning to the query:

4hclA Crystal structure of d-glucarate dehydratase from agrobacterium tumefaciens complexed with magnesium and l-lyxarohydroxamate
33% identity, 82% coverage: 58:366/378 of query aligns to 64:374/384 of 4hclA

query
sites
4hclA

V

I

V

E

K

K

A

F

Y

V

R

K

A

K

A

V

L

L

I

I

G

G

Q

E

D

D

P

H

L

R

Q

D

T

R

E

E

R

R

L

L

W

W

L

Q

E

D

L

L

Y

A

N

H

R

W

L

Q

R

R

D

G

Q

S

G

A

Q

A

R

Q

G

-

L

L

T

T

V

D

T

R

A

T

L

L

S

A

G

V

I

V

D

D

I

C

A

A

L

L

W

W

D

D

I

L

K

A

G

G

K

R

R

S

F

L

G

G

V

Q

P

P

I

V

S

Y

T

K

L

L

L

I

G

G

R

-

F

Y

R

R

D

D

S

K

V

V

R

L

A

A

Y

Y

A

G

T
x
S

-

I

-

M

-
x
C

G

G

S

D

F

E

R

L

R

E

A

G

G

G

V

L

D

A

R

T

V

P

E

E

D

D

N

Y

A

G

R

R

E

F

V

A

A

E

G

T

Y

L

R

V

R

K

E

R

G

G

F

Y

H

K

A

G

V

I

K
|
K

I

L

K
x
H

I

T

G

W

F

M

-

P

-

V

-

S

-

W

-

A

-
x
P

G

D

V

V

D

K

E

M

D

D

L

L

R

K

V

A

I

C

E

A

A

A

V

V

R

R

E

E

S

A

I

V

G

G

P

P

D

D

M

I

R

R

L

L

M

M

I

I

D
|
D

A

A

N
x
F

H
|
H

G

W

Y

Y

D

S

Y

R

L

T

E

D

A

A

V

L

E

A

V

L

G

G

R

R

R

G

A

L

A

E

K

K

F

L

G

G

I

F

D

D

W

W

F

I

E
|
E

E

E

P

P

V

M

L

D

P

E

E

Q

H

S

V

L

S

S

A

S

Y

Y

R

K

A

W

V

L

R
x
S

A

D

D

N

Q
x
L

P

D

I

I

P

P

V

V

A

V

G

G

G
x
P

E
|
E

T

S

W

A

H

A

G

G

R

K

Y

H

A

W

M

H

H

R

-

A

E

E

P

W

L

I

A

K

T

A

R

G

A

A

V

C

D

D

I

I

I

L

Q
x
R

P

T

D
x
G

I

V

C

N

G

D

V

V

G

G

F

I

T

T

E

P

I

A

R

L

R

K

V

T

A

M

D

H

M

L

A

A

A

E

L

A

H

F

G

G

V

M

R

E

L

C

V

E

P

V

H
|
H

V

-

W

-

G
|
G

T
x
N

A

T

V

-

C

-

L

-

A

A

A

M

S

N

L

L

Q

H

F

V

M

V

A

A

L

T

L

K

P

N

-

C

N

R

P

W

P

Y

R
x
E

R

R

D
x
G

P

L

I

L

E

H

P

P

I

F

L

L

E
|
E

F

Y

D

D

R

D

T

G

E

H

N

D

P

Y

F

L

R

K

Q

S

A

-

V

-

V

L

T

S

T

D

P

P

I

M

E

D

H

R

D

D

R

-

G

G

V

F

V

V

A

H

I

V

P

P

D

D

G

R

P

P

G

G

L

L

G

G

I

E

E

D

I

I

D

D

active site: S138 (≠ T134), K171 (= K164), H173 (≠ K166), P183 (vs. gap), D208 (= D194), F210 (≠ N196), E234 (= E220), P259 (≠ G245), E260 (= E246), R282 (≠ Q267), G284 (≠ D269), H311 (= H296), G312 (= G299), N313 (≠ T300), E331 (≠ R320), G333 (≠ D322), E340 (= E329)
binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: C141 (vs. gap), K171 (= K164), H173 (≠ K166), D208 (= D194), H211 (= H197), E260 (= E246), H311 (= H296), E331 (≠ R320)
binding magnesium ion: D208 (= D194), E234 (= E220), S249 (≠ R235), L252 (≠ Q238), E260 (= E246)

Sites not aligning to the query:

binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: 26

4hcdA Crystal structure of d-glucarate dehydratase from agrobacterium tumefaciens complexed with magnesium
33% identity, 82% coverage: 58:366/378 of query aligns to 64:374/384 of 4hcdA

query
sites
4hcdA

V

I

V

E

K

K

A

F

Y

V

R

K

A

K

A

V

L

L

I

I

G

G

Q

E

D

D

P

H

L

R

Q

D

T

R

E

E

R

R

L

L

W

W

L

Q

E

D

L

L

Y

A

N

H

R

W

L

Q

R

R

D

G

Q

S

G

A

Q

A

R

Q

G

-

L

L

T

T

V

D

T

R

A

T

L

L

S

A

G

V

I

V

D

D

I

C

A

A

L

L

W

W

D

D

I

L

K

A

G

G

K

R

R

S

F

L

G

G

V

Q

P

P

I

V

S

Y

T

K

L

L

L

I

G

G

R

-

F

Y

R

R

D

D

S

K

V

V

R

L

A

A

Y

Y

A

G

T
x
S

-

I

-

M

-

C

G

G

S

D

F

E

R

L

R

E

A

G

G

G

V

L

D

A

R

T

V

P

E

E

D

D

N

Y

A

G

R

R

E

F

V

A

A

E

G

T

Y

L

R

V

R

K

E

R

G

G

F

Y

H

K

A

G

V

I

K
|
K

I

L

K
x
H

I

T

G

W

F

M

-

P

-

V

-

S

-

W

-

A

-
x
P

G

D

V

V

D

K

E

M

D

D

L

L

R

K

V

A

I

C

E

A

A

A

V

V

R

R

E

E

S

A

I

V

G

G

P

P

D

D

M

I

R

R

L

L

M

M

I

I

D
|
D

A

A

N
x
F

H

H

G

W

Y

Y

D

S

Y

R

L

T

E

D

A

A

V

L

E

A

V

L

G

G

R

R

R

G

A

L

A

E

K

K

F

L

G

G

I

F

D

D

W

W

F

I

E
|
E

E

E

P

P

V

M

L

D

P

E

E

Q

H

S

V

L

S

S

A

S

Y

Y

R

K

A

W

V

L

R
x
S

A

D

D

N

Q
x
L

P

D

I

I

P

P

V

V

A

V

G

G

G
x
P

E
|
E

T

S

W

A

H

A

G

G

R

K

Y

H

A

W

M

H

H

R

-

A

E

E

P

W

L

I

A

K

T

A

R

G

A

A

V

C

D

D

I

I

I

L

Q
x
R

P

T

D
x
G

I

V

C

N

G

D

V

V

G

G

F

I

T

T

E

P

I

A

R

L

R

K

V

T

A

M

D

H

M

L

A

A

A

E

L

A

H

F

G

G

V

M

R

E

L

C

V

E

P

V

H
|
H

V

-

W

-

G
|
G

T
x
N

A

T

V

-

C

-

L

-

A

A

A

M

S

N

L

L

Q

H

F

V

M

V

A

A

L

T

L

K

P

N

-

C

N

R

P

W

P

Y

R
x
E

R

R

D
x
G

P

L

I

L

E

H

P

P

I

F

L

L

E
|
E

F

Y

D

D

R

D

T

G

E
x
H

N

D

P

Y

F
x
L

R

K

Q

S

A

-

V

-

V

L

T

S

T

D

P

P

I

M

E

D

H

R

D

D

R

-

G

G

V

F

V

V

A

H

I

V

P

P

D

D

G

R

P

P

G

G

L

L

G

G

I

E

E

D

I

I

D

D

active site: S138 (≠ T134), K171 (= K164), H173 (≠ K166), P183 (vs. gap), D208 (= D194), F210 (≠ N196), E234 (= E220), P259 (≠ G245), E260 (= E246), R282 (≠ Q267), G284 (≠ D269), H311 (= H296), G312 (= G299), N313 (≠ T300), E331 (≠ R320), G333 (≠ D322), E340 (= E329)
binding magnesium ion: D208 (= D194), E234 (= E220), S249 (≠ R235), L252 (≠ Q238), E260 (= E246), H345 (≠ E334), L348 (≠ F337)

Query Sequence

>WP_086510675.1 NCBI__GCF_002151265.1:WP_086510675.1
MKIAAVHTHILDHVLDTTFESASMYFARRQHCLVEIVCDDGTIGWGECLGPAQPNAAVVK
AYRAALIGQDPLQTERLWLELYNRLRDQGQRGLTVTALSGIDIALWDIKGKRFGVPISTL
LGGRFRDSVRAYATGSFRRAGVDRVEDNAREVAGYRREGFHAVKIKIGFGVDEDLRVIEA
VRESIGPDMRLMIDANHGYDYLEAVEVGRRAAKFGIDWFEEPVLPEHVSAYRAVRADQPI
PVAGGETWHGRYAMHEPLATRAVDIIQPDICGVGGFTEIRRVADMAALHGVRLVPHVWGT
AVCLAASLQFMAALLPNPPRRDPIEPILEFDRTENPFRQAVVTTPIEHDRGVVAIPDGPG
LGIEIDREALARFALVEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory