SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086510738.1 NCBI__GCF_002151265.1:WP_086510738.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q58761 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
33% identity, 93% coverage: 15:290/297 of query aligns to 13:284/284 of Q58761

query
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Q58761

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DNE 34:36 (≠ DEE 36:38) binding
D212 (= D218) binding
STGGT 216:220 (≠ SSGRT 222:226) binding

1u9zA Crystal structure of phosphoribosyl diphosphate synthase complexed with amp and ribose 5-phosphate (see paper)
33% identity, 92% coverage: 15:286/297 of query aligns to 13:270/274 of 1u9zA

query
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1u9zA

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binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R92 (= R94), Q93 (= Q95), F97 (= F99), H125 (= H127)
binding ribose-5-phosphate: D163 (= D169), D202 (= D218), I205 (≠ A221), S206 (= S222), T207 (≠ S223), G208 (= G224), T210 (= T226)

Q97Z86 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
31% identity, 87% coverage: 16:273/297 of query aligns to 14:271/291 of Q97Z86

query
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Q97Z86

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STGGT 220:224 (≠ SSGRT 222:226) binding

4twbA Sulfolobus solfataricus ribose-phosphate pyrophosphokinase (see paper)
29% identity, 87% coverage: 16:273/297 of query aligns to 14:258/278 of 4twbA

query
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4twbA

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binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R93 (= R94), F98 (= F99), H127 (= H127)

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
28% identity, 88% coverage: 15:276/297 of query aligns to 15:277/316 of 8dbkB

query
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8dbkB

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E

K

A

W

I

I

A

R

E

E

R

N

-

I

-

S

-

E

R

W

R

R

D

N

A

C

L

T

L

I

L

V

G

S

P

P

D
|
D

A

A

E
x
G

S

G

A

A

Q

K

W

R

V

V

E

T

S

S

A

I

A

A

R

D

A

R

T

L

G

N

L

V

A

D

Y

F

G

A

V

L

C

I

T

H

K
|
K

Q

E

R
|
R

H

K

G

K

D

A

R
x
N

E

E

V

V

E

D

I

R

A

M

L

V

P

L

E

V

L

G

A

D

F

V

A

K

G

D

R

R

R

V

V

A

V

I

L

L

L

V

D
|
D

I

M

A

A

S
x
D

S
x
T

G
x
C

R
x
G

T
|
T

L

I

A

C

Q

H

A

A

R

D

R

K

V

L

L

L

A

S

A

A

G

G

A

A

A

T

G

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

S

S

V

R

I

I

R

N

Q

N

A

A

G

C

V

F

E

E

E

A

V

V

W

V

S

V

T

T

D

N

S

T

I

I

P

P

H

Q

Q

E

binding adenosine monophosphate: R95 (= R94), Q96 (= Q95), N199 (≠ R198)
binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38)
binding phosphate ion: S46 (≠ A46), R48 (≠ L48)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H127), D170 (= D169), G172 (≠ E171), K193 (= K192), R195 (= R194), D219 (= D218), D220 (= D219), D223 (≠ S222), T224 (≠ S223), C225 (≠ G224), G226 (≠ R225), T227 (= T226)

8dbeA Human prps1 with adp; hexamer (see paper)
28% identity, 88% coverage: 15:276/297 of query aligns to 15:277/316 of 8dbeA

query
sites
8dbeA

A

S

Q

Q

R

K

L

I

A

A

E

D

A

R

V

L

G

G

I

L

E

E

A

L

L

G

R

K

M

V

E

V

R

T

H

K

R

K

F
|
F

P

S

D
x
N

E

Q

E
|
E

L

T

C

C

L

V

R

E

L

I

P

G

D

E

A

S

E

V

L

R

P

G

E

E

C

D

L

V

V

Y

V

I

Y

V

R

Q

S

S

-

G

L

C

N

G

R

E

P

I

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

L

L

I

L

M

A

I

R

N

H

A

A

C

R

K

E

I

R

A

G

S

V

A

R

S

R

R

L

V

V

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
K

A
x
K

F
x
D

R

K

P
x
S

G
x
R

E

A

L

P

V

I

S

S

Q

A

R

K

L

L

V

V

G

A

G

N

F

M

L

L

A

S

E

V

L

A

F

G

-

A

D

D

A

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

I

P

P

L

V

R
x
D

Q

N

A

-

V

-

A

-

L

L

S
x
Y

A

A

A

E

P

P

A

A

L

V

A

L

E

K

A

W

I

I

A

R

E

E

R

N

-

I

-

S

-

E

R

W

R

R

D

N

A

C

L

T

L

I

L

V

G

S

P

P

D
|
D

A

A

E

G

S

G

A

A

Q

K

W

R

V

V

E

T

S

S

A

I

A

A

R

D

A

R

T

L

G

N

L

V

A

D

Y

F

G

A

V

L

C

I

T

H

K

K

Q

E

R

R

H

K

G

K

D

A

R

N

E

E

V

V

E

D

I

R

A

M

L

V

P

L

E

V

L

G

A

D

F

V

A

K

G

D

R

R

R

V

V

A

V

I

L

L

L

V

D
|
D

I

M

A

A

S
x
D

S
x
T

G

C

R
x
G

T
|
T

L

I

A

C

Q

H

A

A

R

D

R

K

V

L

L

L

A

S

A

A

G

G

A

A

A

T

G

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

S

S

V

R

I

I

R

N

Q

N

A

A

G

C

V

F

E

E

E

A

V

V

W

V

S

V

T

T

D

N

S

T

I

I

P

P

H

Q

Q

E

binding adenosine-5'-diphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R94), Q96 (= Q95), K98 (≠ I97), K99 (≠ A98), D100 (≠ F99), S102 (≠ P101), R103 (≠ G102), H129 (= H127), D142 (≠ R141), Y145 (≠ S147)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H127), D170 (= D169), D219 (= D218), D220 (= D219), D223 (≠ S222), T224 (≠ S223), G226 (≠ R225), T227 (= T226)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 307, 308, 309, 312

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
28% identity, 88% coverage: 15:276/297 of query aligns to 16:278/318 of P60891

query
sites
P60891

A
x
S

Q

Q

R

K

L

I

A

A

E

D

A

R

V

L

G

G

I

L

E

E

A

L

L

G

R

K

M

V

E

V

R

T

H

K

R

K

F

F

P

S

D

N

E

Q

E

E

L

T

C

C

L

V

R

E

L

I

P

G

D

E

A

S

E

V

L

R

P

G

E

E

C
x
D

L

V

V

Y

V

I

Y

V

R

Q

S

S

-

G

L

C

N

G

R

E

P

I

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

L

L

I

L

M

A

I

R

N

H

A

A

C

R

K

E

I

R

A

G

S

V

A

R

S

R

R

L

V

V

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R

R

Q

Q

D

D

I

K

A

K

F

D

R

K

P

S

G

R

E

A

L

P

V

I

S

S

Q

A

R

K

L

L

V

V

G

A

G
x
N

F

M

L

L

A

S

E

V

L

A

-

G

F

A

D

D

A

H

L

I

I

I

T

T

V

M

D

D

P
x
L

H

H

L

A

H
x
S

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

I

P

P

L
x
V

R

D

Q
x
N

A

-

V

-

A

-

L

L

S
x
Y

A

A

A

E

P

P

A

A

L

V

A

L

E

K

A

W

I

I

A

R

E

E

R

N

-

I

-

S

-

E

R

W

R

R

D

N

A

C

L

T

L

I

L

V

G

S

P

P

D

D

A

A

E

G

S

G

A

A

Q

K

W

R

V

V

E

T

S

S

A

I

A

A

R
x
D

A

R

T

L

G

N

L

V

A

D

Y

F

G
x
A

V

L

C

I

T
x
H

K

K

Q

E

R

R

H

K

G

K

D

A

R

N

E

E

V

V

E
x
D

I

R

A

M

L

V

P

L

E

V

L

G

A

D

F

V

A

K

G

D

R

R

R

V

V

A

V

I

L

L

L
x
V

D

D

I

M

A

A

S

D

S

T

G

C

R

G

T

T

L

I

A

C

Q
x
H

A

A

R

D

R

K

V

L

L

L

A

S

A

A

G

G

A

A

A

T

G

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

S

S

V

R

I

I

R

N

Q

N

A

A

G

C

V

F

E

E

E

A

V

V

W

V

S

V

T

T

D

N

S

T

I

I

P

P

H

Q

Q

E

S16 (≠ A15) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (≠ C51) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ G112) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (≠ P126) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ H129) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ L140) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (≠ Q142) mutation to H: No effect on catalytic activity.
Y146 (≠ S147) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ R181) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (≠ G188) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ T191) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E201) to H: in a breast cancer sample; somatic mutation
V219 (≠ L217) to G: in a breast cancer sample; somatic mutation
H231 (≠ Q229) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
29% identity, 88% coverage: 15:276/297 of query aligns to 14:270/305 of 2hcrA

query
sites
2hcrA

A

S

Q

Q

R

K

L

I

A

A

E

D

A

R

V

L

G

G

I

L

E

E

A

L

L

G

R

K

M

V

E

V

R

T

H

K

R

K

F

F

P

S

D

N

E

Q

E

E

L

T

C

C

L

V

R

E

L

I

P

G

D

E

A

S

E

V

L

R

P

G

E

E

C

D

L

V

V

Y

V

I

Y

V

R

Q

S

S

-

G

L

C

N

G

R

E

P

I

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

L

L

I

L

M

A

I

R

N

H

A

A

C

R

K

E

I

R

A

G

S

V

A

R

S

R

R

L

V

V

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
K

A

K

F

D

R

K

P

S

G

R

E

A

L

P

V

I

S

S

Q

A

R

K

L

L

V

V

G

A

G

N

F

M

L

L

A

S

E

V

L

A

F

G

-

A

D

D

A

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

I

P

P

L

V

R

D

Q

N

A

-

V

-

A

-

L

L

S

Y

A

A

A

E

P

P

A

A

L

V

A

L

E

K

A

W

I

I

A

R

E

E

R

N

-

I

-

S

-

E

R

W

R

R

D

N

A

C

L

T

L

I

L

V

G

S

P

P

D

D

A

A

E

G

S

G

A

A

Q

K

W

R

V

V

E

T

S

S

A

I

A

A

R

D

A

R

T

L

G

N

L

V

A

D

Y

F

G

A

V

L

C

I

T

H

K

K

Q

E

R

R

H

-

G

-

D

D

R

R

E

M

V

V

E

L

I

V

A

G

L

-

P

-

E

-

L

-

A

D

F

V

A

K

G

D

R

R

R

V

V

A

V

I

L

L

L

V

D

D

I

M

A

A

S

D

S

T

G

C

R

G

T

T

L

I

A

C

Q

H

A

A

R

D

R

K

V

L

L

L

A

S

A

A

G

G

A

A

A

T

G

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

S

S

V

R

I

I

R

N

Q

N

A

A

G

C

V

F

E

E

E

A

V

V

W

V

S

V

T

T

D

N

S

T

I

I

P

P

H

Q

Q

E

binding adenosine monophosphate: R94 (= R94), Q95 (= Q95), K97 (≠ I97), H128 (= H127)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
28% identity, 88% coverage: 15:276/297 of query aligns to 15:270/309 of 8dbgA

query
sites
8dbgA

A

S

Q

Q

R

K

L

I

A

A

E

D

A

R

V

L

G

G

I

L

E

E

A

L

L

G

R

K

M

V

E

V

R

T

H

K

R

K

F
|
F

P

S

D
x
N

E

Q

E
|
E

L

T

C

C

L

V

R

E

L

I

P

G

D

E

A
x
S

E

V

L
x
R

P

G

E

E

C

D

L

V

V

Y

V

I

Y

V

R

Q

S

S

-

G

L

C

N

G

R

E

P

I

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

L

L

I

L

M

A

I

R

N

H

A

A

C

R

K

E

I

R

A

G

S

V

A

R

S

R

R

L

V

V

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
K

A

K

F

D

R

K

P

S

G

R

E

A

L

P

V

I

S

S

Q

A

R

K

L

L

V

V

G

A

G

N

F

M

L

L

A

S

E

V

L

A

F

G

-

A

D

D

A

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

I

P

P

L

V

R

D

Q

N

A

-

V

-

A

-

L

L

S

Y

A

A

A

E

P

P

A

A

L

V

A

L

E

K

A

W

I

I

A

R

E

E

R

N

-

I

-

S

-

E

R

W

R

R

D

N

A

C

L

T

L

I

L

V

G

S

P

P

D

D

A

A

E

G

S

G

A

A

Q

K

W

R

V

V

E

T

S

S

A

I

A

A

R

D

A

R

T

L

G

N

L

V

A

D

Y

F

G

A

V

L

C

I

T

H

K

K

Q

E

R

-

H

-

G

-

D

D

R

R

E

M

V

V

E

L

I

V

A

G

L

-

P

-

E

-

L

-

A

D

F

V

A

K

G

D

R

R

R

V

V

A

V

I

L

L

L

V

D

D

I

M

A

A

S
x
D

S
x
T

G
x
C

R

G

T
|
T

L

I

A

C

Q

H

A

A

R

D

R

K

V

L

L

L

A

S

A

A

G

G

A

A

A

T

G

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

S

S

V

R

I

I

R

N

Q

N

A

A

G

C

V

F

E

E

E

A

V

V

W

V

S

V

T

T

D

N

S

T

I

I

P

P

H

Q

Q

E

binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R94), Q96 (= Q95), K98 (≠ I97), H129 (= H127)
binding phosphate ion: S46 (≠ A46), R48 (≠ L48), D216 (≠ S222), T217 (≠ S223), C218 (≠ G224), T220 (= T226)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
29% identity, 96% coverage: 5:290/297 of query aligns to 2:293/307 of 5t3oA

query
sites
5t3oA

L

L

Y

I

F

F

D

S

E

G

E

Q

S

S

-

N

-

R

P

P

A

L

A

A

Q

Q

R

A

L

I

A

A

E

E

A

A

V

L

G

G

I

L

E

P

A

L

L

G

R

K

M

S

E

T

R

T

H

L

R

R

F

F

P

A

D

N

E

D

E

N

L

L

C

F

L

V

R

R

L

Y

P

E

D

E

A
x
S

E

L

L
x
R

P

E

E

G

C

D

L

V

V

F

V

I

Y

V

R

Q

S

S

L

F

N

V

R

P

P

P

-

V

N

Q

D

D

K

H

L

L

V

M

E

E

L

L

M

L

L

M

A

V

R

D

H

A

A

A

R

K

E

G

R

A

G

S

V

A

R

A

R

R

L

V

V

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

I

K

A

K

F
x
D

R

A

P

P

G

R

E

I

L

S

V

I

S

T

Q

A

R

R

L

L

V

I

G

A

G

D

F

L

L

L

A

Q

E

T

L

A

-

G

F

A

D

D

A

R

L

V

I

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

I

V

E

H

R

G

L

F

E

F

Q

K

A

-

V

I

P

P

L

V

R

D

Q

H

A

-

V

-

A

-

L

L

S

S

A

A

A

E

P

P

A

V

L

I

A

A

E

N

A

Y

I

F

A

A

E

T

R

R

-

V

-

D

R

L

R

E

D

N

A

A

L

V

G

A

P

P

D

D

A

A

E

G

S

D

A

L

Q

K

W

R

V

A

E

S

S

A

A

L

A

A

R

R

A

R

T

L

G

G

L

L

A

P

Y

L

G

A

V

F

C

I

T

D

K

K

Q

E

R
|
R

H

V

G

S

D

D

R

T

E

E

V

V

E

R

I

V

A

R

L

M

P

L

E

V

L

G

A

E

F

V

A

E

G

G

R

K

R

T

V

A

V

L

L

I

L

V

D

D

I

E

A

I

S

S

S

T

G

A

R

G

T

S

L

L

A

V

Q

E

A

A

A

V

R

E

R

A

V

L

L

M

A

Q

A

A

G

G

A

A

A

K

G

E

V

V

D

Y

V

A

A

A

V

A

T

T

H

H

A

G

L

V

F

Y

A

V

G

G

D

P

A

A

L

L

S

D

V

R

I

I

R

A

Q

K

A

S

G

P

V

V

E

K

E

E

V

V

W

A

S

A

T

T

D

D

S

T

I

C

P

P

H

P

Q

K

S

E

N

G

A

P

V

K

L

L

-

R

-

T

-

L

-

T

L

V

A

A

P

P

A

L

L

F

A

A

A

E

A

A

L

I

binding adenosine-5'-diphosphate: R94 (= R94), S95 (≠ Q95), D99 (≠ F99), H128 (= H127), R193 (= R194)
binding sulfate ion: S45 (≠ A46), R47 (≠ L48)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
29% identity, 96% coverage: 5:290/297 of query aligns to 3:294/312 of 7pn0A

query
sites
7pn0A

L

L

Y

I

F

F

D

S

E

G

E

Q

S

S

-

N

-

R

P

P

A

L

A

A

Q

Q

R

A

L

I

A

A

E

E

A

A

V

L

G

G

I

L

E

P

A

L

L

G

R

K

M

S

E

T

R

T

H

L

R

R

F

F

P

A

D

N

E

D

E

N

L

L

C

F

L

V

R

R

L

Y

P

E

D

E

A

S

E

L

L

R

P

E

E

G

C

D

L

V

V

F

V

I

Y

V

R

Q

S

S

L

F

N

V

R

P

P

P

-

V

N

Q

D

D

K

H

L

L

V

M

E

E

L

L

M

L

L

M

A

V

R

D

H

A

A

A

R

K

E

G

R

A

G

S

V

A

R

A

R

R

L

V

V

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

I

K

A

K

F

D

R

A

P

P

G

R

E

I

L

S

V

I

S

T

Q

A

R

R

L

L

V

I

G

A

G

D

F

L

L

L

A

Q

E

T

L

A

-

G

F

A

D

D

A

R

L

V

I

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

I

V

E

H

R

G

L

F

E

F

Q

K

A

-

V

I

P

P

L

V

R

D

Q

H

A

-

V

-

A

-

L

L

S

S

A

A

A

E

P

P

A

V

L

I

A

A

E

N

A

Y

I

F

A

A

E

T

R

R

-

V

-

D

R

L

R

E

D

N

A

A

L

V

G

A

P

P

D

D

A

A

E

G

S

D

A

L

Q

K

W

R

V

A

E

S

S

A

A

L

A

A

R

R

A

R

T

L

G

G

L

L

A

P

Y

L

G

A

V

F

C

I

T

D

K

K

Q

E

R

R

H

V

G

S

D

D

R

T

E

E

V

V

E

R

I

V

A

R

L

M

P

L

E

V

L

G

A

E

F

V

A

E

G

G

R

K

R

T

V

A

V

L

L

I

L

V

D

D

I

E

A

I

S

S

S

T

G

A

R

G

T

S

L

L

A

V

Q

E

A

A

A

V

R

E

R

A

V

L

L

M

A

Q

A

A

G

G

A

A

A

K

G

E

V

V

D

Y

V

A

A

A

V

A

T

T

H

H

A

G

L

V

F

Y

A

V

G

G

D

P

A

A

L

L

S

D

V

R

I

I

R

A

Q

K

A

S

G

P

V

V

E

K

E

E

V

V

W

A

S

A

T

T

D

D

S

T

I

C

P

P

H

P

Q

K

S

E

N

G

A

P

V

K

L

L

-

R

-

T

-

L

-

T

L

V

A

A

P

P

A

L

L

F

A

A

A

E

A

A

L

I

binding adenosine-5'-diphosphate: R95 (= R94), S96 (≠ Q95), H129 (= H127)

7yk1A Structural basis of human prps2 filaments (see paper)
29% identity, 88% coverage: 15:276/297 of query aligns to 15:268/306 of 7yk1A

query
sites
7yk1A

A

S

Q

Q

R

R

L

V

A

A

E

D

A

R

V

L

G

G

I

L

E

E

A

L

L

G

R

K

M

V

E

V

R

T

H

K

R

K

F
|
F

P

S

D
x
N

E

Q

E
|
E

L

T

C

S

L

V

R

E

L

I

P

G

D

E

A
x
S

E

V

L
x
R

P

G

E

E

C

D

L

V

V

Y

V

I

Y

I

R

Q

S

S

-

G

L

C

N

G

R

E

P

I

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

L

L

I

L

M

A

I

R

N

H

A

A

C

R

K

E

I

R

A

G

S

V

S

R

S

R

R

L

V

V

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q

Q

D

D

I

K

A
x
K

F
x
D

R
x
K

P
x
S

G
x
R

E

A

L

P

V

I

S

S

Q

A

R

K

L

L

V

V

G

A

G

N

F

M

L

L

A

S

E

V

L

A

F

G

-

A

D

D

A

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

I

P

P

L

V

R
x
D

Q

N

A

-

V

-

A

-

L

L

S

Y

A

A

A

E

P

P

A

A

L

V

A

L

E

Q

A

W

I

I

A

R

E

E

R

N

-

I

-

A

-

E

R

W

R

K

D

N

A

C

L

I

L

V

G

S

P

P

D

D

A

A

E

G

S

G

A

A

Q

K

W

R

V

V

E

T

S

S

A

I

A

A

R

-

A

-

T

-

G

-

L

-

A

-

Y

-

G

-

V

-

C

-

T

-

K

-

Q

-

R

-

H

-

G

-

D

D

R

R

-

L

E

N

V

V

E

E

I

F

A

A

L

L

-

I

-

H

P

K

E

E

L

M

A

V

F

L

A

V

G

G

-

D

-

V

-

K

-

D

R

R

R

V

V

A

V

I

L

L

L

V

D

D

I

M

A

A

S
x
D

S

T

G
x
C

R

G

T
|
T

L

I

A

C

Q

H

A

A

R

D

R

K

V

L

L

L

A

S

A

A

G

G

A

A

A

T

G

K

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

S

S

V

R

I

I

R

N

Q

N

A

A

G

A

V

F

E

E

E

A

V

V

W

V

S

V

T

T

D

N

S

T

I

I

P

P

H

Q

Q

E

binding adenosine-5'-diphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), S46 (≠ A46), R48 (≠ L48), R95 (= R94), K99 (≠ A98), D100 (≠ F99), K101 (≠ R100), S102 (≠ P101), R103 (≠ G102), H129 (= H127), D142 (≠ R141)
binding phosphate ion: D214 (≠ S222), C216 (≠ G224), T218 (= T226)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 298, 300, 303

3mbiA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
25% identity, 87% coverage: 15:273/297 of query aligns to 15:266/287 of 3mbiA

query
sites
3mbiA

A

A

Q

A

R

R

L

I

A

A

E

E

A

E

V

L

G

K

I

T

E

E

A

P

L

V

R

M

M

P

E

D

R

E

H

R

R

R

F
|
F

P

P

D
|
D

E

G

E
|
E

L

L

C

Y

L

L

R

R

L

Y

P

-

D

D

A

E

E

D

L

L

P

T

E

G

C

H

L

N

V

I

V

F

Y

I

R

I

S

G

L

N

N

T

R

H

P

S

N

D

D

A

K

E

L

V

V

M

E

E

L

M

M

I

L

L

L

T

A

L

R

S

H

A

A

I

R

Q

E

D

R

Y

G

R

V

T

R

K

R

S

L

V

V

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

I

Q

A

R

F
x
Y

R

K

P

N

G

G

E

E

L

P

V

I

S

S

Q

S

R

Q

L

I

V

L

G

T

G

E

F

I

L

Y

A

S

E

S

L

Y

F

S

D

N

A

S

L

I

I

A

T

T

V

V

D

D

P

I

H
|
H

L

-

H

-

R

-

I

-

E

-

R

-

L

-

E

D

Q

E

A

K

V

T

P

L

L

S

R

Y

Q

S

A

K

V

V

A

K

L

F

S

S

A

D

A

L

P

H

A

A

L

-

A

N

E

D

A

A

I

I

A

V

E

R

R

Y

R

K

D

N

A

V

-

D

-

V

-

D

L

Y

L

V

G

S

P

P

D

D

A

D

E

G

S

G

A

L

Q

A

W

R

V

V

E

A

S

D

A

I

A

S

R

A

A

K

T

L

G

G

L

K

A

K

Y

H

G

F

V

F

C

I

T

E

K
|
K

Q

K

R
|
R

H

I

G

D

D

D

R

R

E

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

A

D

F

V

A

N

G

G

R

K

V

L

L

I

L

V

D

D

I

I

A

I

S
|
S

S
x
T

G
|
G

R

G

T
|
T

L

I

A

A

Q

K

A

S

R

G

R

L

V

L

L

R

A

E

A

K

G

G

A

A

A

S

G

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

G

N

D

G

A

S

L

E

S

N

V

K

I

I

R

L

Q

Q

A

-

G

N

V

A

E

D

E

E

V

I

W

H

S

V

T

T

D

D

S

T

I

V

binding adenosine-5'-diphosphate: F34 (= F34), D36 (= D36), E38 (= E38), R93 (= R94), Q94 (= Q95), Y98 (≠ F99), H126 (= H127), K186 (= K192), R188 (= R194)
binding magnesium ion: H95 (≠ D96), H126 (= H127)
binding phosphate ion: R188 (= R194), S216 (= S222), T217 (≠ S223), G218 (= G224), T220 (= T226)

3mbiD Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
25% identity, 87% coverage: 15:273/297 of query aligns to 13:264/285 of 3mbiD

query
sites
3mbiD

A

A

Q

A

R

R

L

I

A

A

E

E

A

E

V

L

G

K

I

T

E

E

A

P

L

V

R

M

M

P

E

D

R

E

H

R

R

R

F
|
F

P

P

D
|
D

E

G

E
|
E

L

L

C

Y

L

L

R

R

L

Y

P

-

D

D

A

E

E

D

L

L

P

T

E

G

C

H

L

N

V

I

V

F

Y

I

R

I

S

G

L

N

N

T

R

H

P

S

N

D

D

A

K

E

L

V

V

M

E

E

L

M

M

I

L

L

L

T

A

L

R

S

H

A

A

I

R

Q

E

D

R

Y

G

R

V

T

R

K

R

S

L

V

V

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

H

I

Q

A

R

F
x
Y

R

K

P

N

G

G

E

E

L

P

V

I

S

S

Q

S

R

Q

L

I

V

L

G

T

G

E

F

I

L

Y

A

S

E

S

L

Y

F

S

D

N

A

S

L

I

I

A

T

T

V

V

D

D

P

I

H
|
H

L

-

H

-

R

-

I

-

E

-

R

-

L

-

E

D

Q

E

A

K

V

T

P

L

L

S

R

Y

Q

S

A

K

V

V

A

K

L

F

S

S

A

D

A

L

P

H

A

A

L

-

A

N

E

D

A

A

I

I

A

V

E

R

R

Y

R

K

D

N

A

V

-

D

-

V

-

D

L

Y

L

V

G

S

P

P

D

D

A

D

E

G

S

G

A

L

Q

A

W

R

V

V

E

A

S

D

A

I

A

S

R

A

A

K

T

L

G

G

L

K

A

K

Y

H

G

F

V

F

C

I

T

E

K
|
K

Q

K

R
|
R

H

I

G

D

D

D

R

R

E

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

A

D

F

V

A

N

G

G

R

K

V

L

L

I

L

V

D
|
D

I
|
I

A

I

S
|
S

S
x
T

G
|
G

R

G

T
|
T

L

I

A

A

Q

K

A

S

R

G

R

L

V

L

L

R

A

E

A

K

G

G

A

A

A

S

G

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

G

N

D

G

A

S

L

E

S

N

V

K

I

I

R

L

Q

Q

A

-

G

N

V

A

E

D

E

E

V

I

W

H

S

V

T

T

D

D

S

T

I

V

binding adenosine-5'-diphosphate: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), Y96 (≠ F99), H124 (= H127), K184 (= K192), R186 (= R194)
binding 5-O-phosphono-alpha-D-ribofuranose: R91 (= R94), H124 (= H127), K184 (= K192), R186 (= R194), D210 (= D218), D211 (= D219), I212 (= I220), S214 (= S222), T215 (≠ S223), G216 (= G224), T218 (= T226)

Q97CA5 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (see paper)
25% identity, 87% coverage: 15:273/297 of query aligns to 13:264/286 of Q97CA5

query
sites
Q97CA5

A

A

Q

A

R

R

L

I

A

A

E

E

A

E

V

L

G

K

I

T

E

E

A

P

L

V

R

M

M

P

E

D

R

E

H

R

R

R

F

F

P

P

D
|
D

E
x
G

E
|
E

L

L

C

Y

L

L

R

R

L

Y

P

-

D

D

A

E

E

D

L

L

P

T

E

G

C

H

L

N

V

I

V

F

Y

I

R

I

S

G

L

N

N

T

R

H

P

S

N

D

D

A

K

E

L

V

V

M

E

E

L

M

M

I

L

L

L

T

A

L

R

S

H

A

A

I

R

Q

E

D

R

Y

G

R

V

T

R

K

R

S

L

V

V

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

I

Q

A

R

F

Y

R

K

P

N

G

G

E

E

L

P

V

I

S

S

Q

S

R

Q

L

I

V

L

G

T

G

E

F

I

L

Y

A

S

E

S

L

Y

F

S

D

N

A

S

L

I

I

A

T

T

V

V

D

D

P

I

H

H

L

-

H

-

R

-

I

-

E

-

R

-

L

-

E

D

Q

E

A

K

V

T

P

L

L

S

R

Y

Q

S

A

K

V

V

A

K

L

F

S

S

A

D

A

L

P

H

A

A

L

-

A

N

E

D

A

A

I

I

A

V

E

R

R

Y

R

K

D

N

A

V

-

D

-

V

-

D

L

Y

L

V

G

S

P

P

D

D

A

D

E

G

S

G

A

L

Q

A

W

R

V

V

E

A

S

D

A

I

A

S

R

A

A

K

T

L

G

G

L

K

A

K

Y

H

G

F

V

F

C

I

T

E

K

K

Q

K

R
|
R

H

I

G

D

D

D

R

R

E

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

A

D

F

V

A

N

G

G

R

K

V

L

L

I

L

V

D
|
D

D

D

I

I

A

I

S
|
S

S
x
T

G
|
G

R
x
G

T
|
T

L

I

A

A

Q

K

A

S

R

G

R

L

V

L

L

R

A

E

A

K

G

G

A

A

A

S

G

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

G

N

D

G

A

S

L

E

S

N

V

K

I

I

R

L

Q

Q

A

-

G

N

V

A

E

D

E

E

V

I

W

H

S

V

T

T

D

D

S

T

I

V

DGE 34:36 (≠ DEE 36:38) binding
RQH 91:93 (≠ RQD 94:96) binding
R186 (= R194) binding
D210 (= D218) binding
STGGT 214:218 (≠ SSGRT 222:226) binding

3lpnA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with an atp analog (ampcpp). (see paper)
25% identity, 87% coverage: 15:273/297 of query aligns to 13:264/284 of 3lpnA

query
sites
3lpnA

A

A

Q

A

R
|
R

L

I

A

A

E

E

A

E

V

L

G

K

I

T

E

E

A

P

L

V

R

M

M

P

E

D

R

E

H

R

R

R

F
|
F

P

P

D
|
D

E

G

E
|
E

L

L

C

Y

L

L

R

R

L

Y

P

-

D

D

A

E

E

D

L

L

P

T

E

G

C

H

L

N

V

I

V

F

Y

I

R

I

S

G

L

N

N

T

R

H

P
x
S

N

D

D

A

K

E

L

V

V

M

E

E

L

M

M

I

L

L

L

T

A

L

R

S

H

A

A

I

R

Q

E

D

R

Y

G

R

V

T

R

K

R

S

L

V

V

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

I

Q

A

R

F
x
Y

R

K

P

N

G

G

E

E

L

P

V

I

S

S

Q

S

R

Q

L

I

V

L

G

T

G

E

F

I

L

Y

A

S

E

S

L

Y

F

S

D

N

A

S

L

I

I

A

T

T

V

V

D

D

P

I

H
|
H

L

-

H

-

R

-

I

-

E

-

R

-

L

-

E
x
D

Q

E

A

K

V

T

P

L

L

S

R

Y

Q

S

A

K

V

V

A

K

L

F

S

S

A

D

A

L

P

H

A

A

L

-

A

N

E

D

A

A

I

I

A

V

E

R

R

Y

R

K

D

N

A

V

-

D

-

V

-

D

L

Y

L

V

G

S

P

P

D

D

A

D

E

G

S

G

A

L

Q

A

W

R

V

V

E

A

S

D

A

I

A

S

R

A

A

K

T

L

G

G

L

K

A

K

Y

H

G

F

V

F

C

I

T

E

K

K

Q

K

R

R

H

I

G

D

D

D

R

R

E

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

A

D

F

V

A

N

G

G

R

K

V

L

L

I

L

V

D

D

I

I

A

I

S
|
S

S
x
T

G
|
G

R

G

T
|
T

L

I

A

A

Q

K

A

S

R

G

R

L

V

L

L

R

A

E

A

K

G

G

A

A

A

S

G

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

G

N

D

G

A

S

L

E

S

N

V

K

I

I

R

L

Q

Q

A

-

G

N

V

A

E

D

E

E

V

I

W

H

S

V

T

T

D

D

S

T

I

V

binding diphosphomethylphosphonic acid adenosyl ester: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), H93 (≠ D96), Y96 (≠ F99), H124 (= H127), D125 (≠ E135)
binding sulfate ion: R15 (= R17), S58 (≠ P61), S214 (= S222), T215 (≠ S223), G216 (= G224), T218 (= T226)

Sites not aligning to the query:

binding sulfate ion: 7, 11

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 88% coverage: 15:275/297 of query aligns to 18:280/321 of O94413

query
sites
O94413

A

A

Q

E

R

K

L

V

A

A

E

R

A

R

V

I

G

G

I

L

E

S

A

L

L

G

R

K

M

V

E

A

R

V

H

V

R

Q

F

Y

P

S

D

N

E

R

E

E

L

T

C

S

L

V

R

T

L

I

P

G

D

E

A

S

E

V

L

R

P

D

E

E

C

D

L

V

V

F

V

I

Y

L

R

Q

S

T

-

G

L

C

N

G

R

S

P

I

N

N

D

D

K

H

L

L

V

M

E

E

L

L

M

L

L

I

L

M

A

I

R

N

H

A

A

C

R

R

E

S

R

A

G

S

V

A

R

R

L

I

V

T

L

A

V

I

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R

R

Q

Q

D

D

I

K

A

K

F

D

R

K

P

S

G

R

E

A

L

P

V

I

S

T

Q

A

R

R

L

L

V

V

G

A

G

N

F

M

L

L

A

Q

E

T

L

A

-

G

F

C

D

N

A

H

L

I

I

I

T

T

V

M

D

D

P

L

H

H

L

A

H

S

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

N

A

-

V

V

P

P

L

V

R

D

Q

N

A

-

V

-

A

-

L

L

S

Y

A

A

A

E

P

P

A

S

-

V

-

L

-

R

-

Y

L

I

A

R

E

E

A

N

I

I

A

D

E

T

R

T

R

V

R

N

D

P

A

T

L

V

L

I

L

V

G
x
S

P

P

D

D

A

A

E

G

S

G

A

A

Q

K

W

R

V

A

E

T

S

A

A

L

A

A

R

D

A

R

T

L

G

D

L

L

A

D

Y

F

G

A

V

L

C

I

T

H

K

K

Q

E

R

R

H

Q

G

K

D

A

R

N

E

E

V

V

E

S

I

R

A

M

L

V

P

L

E

V

L

G

A

D

F

V

A

R

G

D

R

K

R

L

V

A

V

I

L

L

L

V

D

D

I

M

A

A

S

D

S

T

G

C

R

G

T

T

L

L

A

G

Q

L

A

A

R

K

R

T

V

L

L

K

A

D

A

N

G

G

A

A

A

K

G

A

V

V

D

Y

V

A

A

I

V

V

T

T

H

H

A

G

L

I

F

L

A

S

G

G

D

K

A

A

L

I

S

K

V

V

I

I

R

N

Q

E

A

S

G

A

V

L

E

E

E

K

V

V

W

I

S

V

T

T

D

N

S

T

I

I

P

P

H

H

S172 (≠ G167) modified: Phosphoserine

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
28% identity, 88% coverage: 15:274/297 of query aligns to 18:268/307 of 7xmvA

query
sites
7xmvA

A

A

Q

N

R

R

L

L

A

Y

E

T

A

S

V

L

G

G

I

D

E

A

A

A

L

V

R

-

M

-

E

-

R

-

H

G

R

R

F
|
F

P

S

D
|
D

E

G

E
|
E

L

V

C

S

L

V

R

Q

L

I

P

N

D

E

A

N

E

V

L

R

P

G

E

G

C

D

L

I

V

F

V

I

Y

I

R

Q

S

S

L

T

N

C

R

A

P

P

-

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

V

L

V

L

M

A

V

R

D

H

A

A

L

R

R

E

R

R

A

G

S

V

A

R

G

R

R

L

I

V

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R
|
R

Q

Q

D

D

I
x
R

A

R

F
x
V

R
|
R

P

S

G

A

E

R

L

V

-

P

V

I

S

T

Q

A

R

K

L

V

V

V

G

A

G

D

F

F

L

L

A

S

E

S

L

V

-

G

F

V

D

D

A

R

L

V

I

L

T

T

V

V

D

D

P

L

H
|
H

L

A

H
x
E

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

V

P

P

L

V

R

D

Q

N

A

V

V
x
F

A

G

L

-

S

-

A

-

A
x
S

P

P

A

I

L

L

A

L

E

E

A

D

I

M

A

L

E

Q

R

L

R

N

R

L

D

D

A

N

-

P

L

I

L

V

G

S

P

P

D

D

A

I

E

G

S

G

A

V

Q
x
V

W

R

V

A

E

R

S
x
A

A

I

A

A

R

K

A

L

T

L

G

N

L

D

A

T

Y

D

G

M

V

A

C

I

T

I

K

D

Q

K

R

R

H

-

G

-

D

-

R

R

E

Q

V

V

E

M

I

H

A

I

L

I

P

G

E

D

L

V

A

A

F

-

A

-

G

G

R

R

R

D

V

C

V

V

L

L

L

V

D
|
D

I
x
M

A

I

S
x
D

S
x
T

G

G

R
x
G

T
|
T

L

L

A

C

Q

K

A

A

R

E

R

A

V

L

L

K

A

E

A

R

G

G

A

A

A

K

G

R

V

V

D

F

V

A

A

Y

V

A

T

T

H

H

A

P

L

I

F

F

A

S

G

G

D

N

A

A

L

A

S

N

V

N

I

L

R

R

Q

N

A

S

G

V

V

I

E

D

E

E

V

V

W

V

S

V

T

C

D

D

S

T

I

I

P

P

binding adenosine-5'-diphosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R94), R97 (≠ I97), H129 (= H127)
binding adenosine monophosphate: R97 (≠ I97), V99 (≠ F99), R100 (= R100), E131 (≠ H129), F145 (≠ V144), S147 (≠ A149), V173 (≠ Q174), A177 (≠ S178)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D218), D213 (= D219), M214 (≠ I220), D216 (≠ S222), T217 (≠ S223), G219 (≠ R225), T220 (= T226)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
28% identity, 88% coverage: 15:274/297 of query aligns to 18:268/307 of 7xmuA

query
sites
7xmuA

A

A

Q

N

R

R

L

L

A

Y

E

T

A

S

V

L

G

G

I

D

E

A

A

A

L

V

R

-

M

-

E

-

R

-

H

G

R

R

F
|
F

P

S

D
|
D

E

G

E
|
E

L

V

C

S

L

V

R

Q

L

I

P

N

D

E

A

N

E

V

L

R

P

G

E

G

C

D

L

I

V

F

V

I

Y

I

R

Q

S

S

L

T

N

C

R

A

P

P

-

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

V

L

V

L

M

A

V

R

D

H

A

A

L

R

R

E

R

R

A

G

S

V

A

R

G

R

R

L

I

V

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
R

A

R

F

V

R
|
R

P

S

G

A

E

R

L

V

-

P

V

I

S

T

Q

A

R

K

L

V

V

V

G

A

G

D

F

F

L

L

A

S

E

S

L

V

-

G

F

V

D

D

A

R

L

V

I

L

T

T

V

V

D

D

P

L

H
|
H

L

A

H
x
E

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

V

P

P

L

V

R

D

Q

N

A

V

V
x
F

A

G

L

-

S

-

A

-

A
x
S

P

P

A

I

L

L

A

L

E

E

A

D

I

M

A

L

E

Q

R

L

R

N

R

L

D

D

A

N

-

P

L

I

L

V

G

S

P

P

D
|
D

A

I

E

G

S

G

A

V

Q
x
V

W

R

V

A

E

R

S

A

A

I

A

A

R

K

A

L

T

L

G

N

L

D

A

T

Y

D

G

M

V

A

C

I

T

I

K

D

Q

K

R

R

H

-

G

-

D

-

R

R

E

Q

V

V

E

M

I

H

A

I

L

I

P

G

E

D

L

V

A

A

F

-

A

-

G

G

R

R

R

D

V

C

V

V

L

L

L

V

D
|
D

D

D

I
x
M

A

I

S
x
D

S
x
T

G

G

R

G

T

T

L

L

A

C

Q

K

A

A

R

E

R

A

V

L

L

K

A

E

A

R

G

G

A

A

A

K

G

R

V

V

D

F

V

A

A

Y

V

A

T

T

H

H

A

P

L

I

F

F

A

S

G

G

D

N

A

A

L

A

S

N

V

N

I

L

R

R

Q

N

A

S

G

V

V

I

E

D

E

E

V

V

W

V

S

V

T

C

D

D

S

T

I

I

P

P

binding adenosine-5'-diphosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R94), Q95 (= Q95), R97 (≠ I97), R97 (≠ I97), R100 (= R100), H129 (= H127), E131 (≠ H129), F145 (≠ V144), S147 (≠ A149), V173 (≠ Q174)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D169), D212 (= D218), M214 (≠ I220), D216 (≠ S222), T217 (≠ S223)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
27% identity, 88% coverage: 15:274/297 of query aligns to 20:276/315 of P0A717

query
sites
P0A717

A

A

Q

N

R

R

L

L

A

Y

E

T

A

S

V

L

G

G

I

D

E

A

A

A

L

V

R

-

M

-

E

-

R

-

H

G

R

R

F

F

P

S

D

D

E

G

E

E

L

V

C

S

L

V

R

Q

L

I

P

N

D

E

A

N

E

V

L

R

P

G

E

G

C

D

L

I

V

F

V

I

Y

I

R

Q

S

S

L

T

N

C

R

A

P

P

-

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

M

V

L

V

L

M

A

V

R

D

H

A

A

L

R

R

E

R

R

A

G

S

V

A

R

G

R

R

L

I

V

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

I

R

A

R

F

V

R

R

P

S

G

A

E

R

L

V

-

P

V

I

S

T

Q

A

R

K

L

V

V

V

G

A

G

D

F

F

L

L

A

S

E

S

L

V

-

G

F

V

D

D

A

R

L

V

I

L

T

T

V

V

D
|
D

P

L

H

H

L

A

H

E

R

Q

I

I

E

Q

R

G

L

F

E

F

Q

D

A

-

V

V

P

P

L

V

R

D

Q

N

A

V

V

F

A

G

L

-

S

-

A

-

A

S

P

P

A

I

L

L

A

L

E

E

A

D

I

M

A

L

E

Q

R

L

R

N

R

L

D

D

A

N

-

P

L

I

L

V

G

S

P

P

D

D

A

I

E

G

S

G

A

V

Q

V

W

R

V

A

E

R

S

A

A

I

A

A

R

K

A

L

T

L

G

N

L

D

A

T

Y

D

G

M

V

A

C

I

T

I

K

D

Q

K

R

R

H

R

G

P

D

R

R

A

E

N

V

V

E

S

I

Q

A

V

L

M

P

H

E

I

L

I

A

G

-

D

F

V

A

A

G

G

R

R

R

D

V

C

V

V

L

L

L

V

D
|
D

I

M

A

I

S
x
D

S

T

G

G

R

G

T

T

L

L

A

C

Q

K

A

A

R

E

R

A

V

L

L

K

A

E

A

R

G

G

A

A

A

K

G

R

V

V

D

F

V

A

A

Y

V

A

T

T

H

H

A

P

L

I

F

F

A

S

G

G

D

N

A

A

L

A

S

N

V

N

I

L

R

R

Q

N

A

S

G

V

V

I

E

D

E

E

V

V

W

V

S

V

T

C

D

D

S

T

I

I

P

P

D129 (= D125) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D218) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D219) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ S222) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>WP_086510738.1 NCBI__GCF_002151265.1:WP_086510738.1
MQVSLLYFDEESPAAQRLAEAVGIEALRMERHRFPDEELCLRLPDAELPECLVVYRSLNR
PNDKLVELMLLARHARERGVRRLVLVAPYLAYMRQDIAFRPGELVSQRLVGGFLAELFDA
LITVDPHLHRIERLEQAVPLRQAVALSAAPALAEAIAERRRDALLLGPDAESAQWVESAA
RATGLAYGVCTKQRHGDREVEIALPELAFAGRRVVLLDDIASSGRTLAQAARRVLAAGAA
GVDVAVTHALFAGDALSVIRQAGVEEVWSTDSIPHQSNAVLLAPALAAALGPLLDVA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory