SitesBLAST
Comparing WP_086510929.1 NCBI__GCF_002151265.1:WP_086510929.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
34% identity, 99% coverage: 4:545/549 of query aligns to 3:450/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G12), G13 (= G14), S14 (≠ T15), A15 (= A16), E35 (= E36), A36 (= A37), W47 (= W63), P65 (= P81), G67 (= G83), V180 (= V231), A214 (= A269), G215 (= G270), A218 (≠ G273), T270 (≠ M352), Y391 (≠ F484), A424 (= A519), I435 (≠ T530), N436 (= N531)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
36% identity, 99% coverage: 4:545/549 of query aligns to 82:636/654 of A0A248QE08
- TA 93:94 (= TA 15:16) binding
- E114 (= E36) binding
- L162 (≠ T85) binding
- S166 (≠ C89) binding
- NATL 170:173 (≠ NGML 93:96) binding
- V298 (= V231) binding
- C432 (≠ S355) binding
- R451 (≠ H375) binding
- Y466 (vs. gap) binding
- Q486 (≠ S397) binding
- G622 (≠ N531) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
36% identity, 99% coverage: 4:545/549 of query aligns to 6:560/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H375), N499 (≠ F484)
- binding flavin-adenine dinucleotide: G14 (= G12), G16 (= G14), T17 (= T15), A18 (= A16), L37 (≠ I35), E38 (= E36), A39 (= A37), F58 (≠ I56), W64 (= W63), A82 (≠ P81), G89 (= G88), S90 (≠ C89), N94 (= N93), A95 (≠ G94), T96 (≠ M95), L97 (= L96), M191 (≠ N199), V222 (= V231), C264 (≠ A268), A265 (= A269), G266 (= G270), H269 (≠ G273), N499 (≠ F484), A534 (= A519), Q544 (≠ N529), T545 (= T530), G546 (≠ N531)
- binding heptadecane: V377 (≠ Q377), G379 (vs. gap), M380 (≠ L379), G386 (= G385), T389 (vs. gap), Y390 (vs. gap), F393 (vs. gap), T408 (= T395), Q410 (≠ S397)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
36% identity, 99% coverage: 4:545/549 of query aligns to 22:569/578 of 5nccA
- active site: R347 (≠ K337), L420 (≠ V398), I421 (≠ C399), S507 (≠ I483), A509 (≠ H485), G552 (= G528), Q553 (≠ N529)
- binding flavin-adenine dinucleotide: G30 (= G12), G32 (= G14), T33 (= T15), A34 (= A16), L53 (≠ I35), E54 (= E36), A55 (= A37), F74 (≠ I56), W80 (= W63), A98 (≠ P81), G100 (= G83), G105 (= G88), S106 (≠ C89), N110 (= N93), A111 (≠ G94), T112 (≠ M95), L113 (= L96), V238 (= V231), A278 (= A269), H282 (≠ G273), L286 (= L277), N508 (≠ F484), Q553 (≠ N529), T554 (= T530), G555 (≠ N531), V558 (≠ T534)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
35% identity, 100% coverage: 1:549/549 of query aligns to 9:530/532 of 4mjwA
- active site: I333 (≠ L357), P377 (= P403), N378 (≠ T404), V464 (≠ I483), H466 (= H485), V509 (≠ G528), N510 (= N529)
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), S23 (≠ T15), E44 (= E36), A45 (= A37), W71 (= W63), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (= C89), H99 (≠ I92), N100 (= N93), S101 (≠ G94), I103 (≠ L96), R231 (≠ Q230), A232 (≠ V231), T269 (≠ A269), G270 (= G270), D273 (≠ G273), Y465 (≠ F484), H466 (= H485), A500 (= A519), N510 (= N529), P511 (≠ T530), N512 (= N531), V515 (≠ T534)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
35% identity, 100% coverage: 1:549/549 of query aligns to 9:530/530 of 3ljpA
- active site: I333 (≠ L357), P377 (= P403), N378 (≠ T404), A464 (≠ I483), H466 (= H485), V509 (≠ G528), N510 (= N529)
- binding dihydroflavine-adenine dinucleotide: G22 (= G14), S23 (≠ T15), E44 (= E36), A45 (= A37), W71 (= W63), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (= C89), H99 (≠ I92), N100 (= N93), S101 (≠ G94), I103 (≠ L96), A232 (≠ V231), T269 (≠ A269), D273 (≠ G273), Y465 (≠ F484), H466 (= H485), D499 (= D518), A500 (= A519), N510 (= N529), P511 (≠ T530), N512 (= N531), V515 (≠ T534)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
35% identity, 99% coverage: 1:545/549 of query aligns to 9:526/527 of 2jbvA
- active site: I333 (≠ L357), P377 (= P403), N378 (≠ T404), V464 (≠ I483), H466 (= H485), V509 (≠ G528), N510 (= N529)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G14), S23 (≠ T15), E44 (= E36), A45 (= A37), W71 (= W63), A90 (≠ G83), G95 (= G88), C96 (= C89), H99 (≠ I92), N100 (= N93), S101 (≠ G94), I103 (≠ L96), R231 (≠ Q230), A232 (≠ V231), T269 (≠ A269), G270 (= G270), D273 (≠ G273), V464 (≠ I483), Y465 (≠ F484), H466 (= H485), D499 (= D518), A500 (= A519), N510 (= N529), P511 (≠ T530), N512 (= N531), V515 (≠ T534)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
32% identity, 99% coverage: 5:545/549 of query aligns to 1:561/565 of 5oc1A
- active site: V339 (≠ M340), N413 (= N400), A414 (vs. gap), I499 (= I483), H501 (= H485), A544 (≠ G528), H545 (≠ N529)
- binding 4-methoxybenzoic acid: Y91 (≠ G94), I356 (vs. gap), I390 (≠ P378), F396 (= F384), T412 (≠ C399), I499 (= I483), H501 (= H485), H545 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), N11 (≠ T15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (≠ C89), H90 (≠ N93), Y91 (≠ G94), V93 (≠ L96), V230 (= V231), S270 (≠ A268), A271 (= A269), G272 (= G270), F500 (= F484), H545 (≠ N529), T546 (= T530), Q547 (≠ N531), I550 (≠ T534)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
31% identity, 99% coverage: 5:545/549 of query aligns to 1:561/565 of 3fimB
- active site: V339 (≠ M340), N413 (= N400), A414 (vs. gap), I499 (= I483), H501 (= H485), A544 (≠ G528), H545 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G12), N11 (≠ T15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (≠ C89), H90 (≠ N93), Y91 (≠ G94), V93 (≠ L96), V230 (= V231), S270 (≠ A268), A271 (= A269), F500 (= F484), H501 (= H485), H545 (≠ N529), T546 (= T530), Q547 (≠ N531), I550 (≠ T534)
8bxlB Patulin synthase from penicillium expansum (see paper)
32% identity, 99% coverage: 4:545/549 of query aligns to 12:586/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), T23 (= T15), A24 (= A16), E44 (= E36), A45 (= A37), W80 (= W63), G100 (= G83), G105 (= G88), S106 (≠ C89), R109 (≠ I92), N110 (= N93), Y111 (≠ G94), A113 (≠ L96), L253 (≠ Q230), A254 (≠ V231), A288 (= A269), Q292 (≠ G273), F525 (= F484), D559 (= D518), A560 (= A519), H570 (≠ N529), P571 (≠ T530), Q572 (≠ N531), L575 (≠ T534)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
34% identity, 99% coverage: 1:546/549 of query aligns to 1:528/531 of E4QP00
- V101 (≠ I92) mutation to H: Abolishes activity.
- M103 (≠ G94) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (= V398) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ N400) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I483) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ F484) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H485) mutation to A: Abolishes activity.
- N511 (= N529) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
34% identity, 99% coverage: 6:546/549 of query aligns to 2:524/525 of 4udqA
- active site: L331 (≠ I338), F364 (≠ C399), W365 (≠ N400), V461 (≠ I483), H463 (= H485), A506 (≠ G528), N507 (= N529)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), T11 (= T15), A12 (= A16), E32 (= E36), A33 (= A37), W64 (vs. gap), G88 (= G83), G93 (= G88), G94 (≠ C89), N98 (= N93), M99 (≠ G94), V101 (≠ L96), V229 (= V231), T261 (≠ A268), A262 (= A269), W462 (≠ F484), H463 (= H485), A497 (= A519), N507 (= N529), T508 (= T530), N509 (= N531), T512 (= T534)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
33% identity, 98% coverage: 7:542/549 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ V398), G361 (≠ C399), H444 (≠ I483), H446 (= H485), G487 (= G528), P488 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (≠ T15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W63), R77 (= R82), G78 (= G83), G83 (= G88), S84 (≠ C89), L87 (≠ I92), H88 (≠ N93), A89 (≠ G94), M90 (= M95), G91 (≠ L96), V218 (= V231), A251 (= A269), G252 (= G270), E255 (≠ G273), H445 (≠ F484), A478 (= A519), P488 (≠ N529), I489 (≠ T530), H490 (≠ N531)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G94), S314 (≠ Q356), H444 (≠ I483), H446 (= H485)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
33% identity, 98% coverage: 7:542/549 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ V398), G361 (≠ C399), H444 (≠ I483), H446 (= H485), G487 (= G528), P488 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (≠ T15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W63), R77 (= R82), G78 (= G83), R79 (≠ K84), G83 (= G88), S84 (≠ C89), H88 (≠ N93), A89 (≠ G94), G91 (≠ L96), R217 (≠ Q230), V218 (= V231), A251 (= A269), E255 (≠ G273), H445 (≠ F484), A478 (= A519), P488 (≠ N529), I489 (≠ T530), H490 (≠ N531)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
30% identity, 99% coverage: 5:548/549 of query aligns to 40:600/602 of Q3L245
- N100 (≠ C64) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I92) modified: Tele-8alpha-FAD histidine
- N344 (= N306) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H485) active site, Proton acceptor
- H581 (≠ N529) active site
Sites not aligning to the query:
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
30% identity, 99% coverage: 5:548/549 of query aligns to 15:575/577 of 4h7uA
- active site: A343 (= A330), V426 (≠ C399), Y510 (≠ I483), H512 (= H485), A555 (≠ G528), H556 (≠ N529)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G12), G24 (= G14), T25 (= T15), A26 (= A16), E46 (= E36), A47 (= A37), W74 (= W63), G99 (= G88), C100 (= C89), H103 (≠ I92), N104 (= N93), G105 (= G94), V107 (≠ L96), L242 (≠ Q230), V243 (= V231), G282 (≠ A269), G283 (= G270), A286 (≠ G273), H512 (= H485), A546 (= A519), H556 (≠ N529), T557 (= T530), Q558 (≠ N531), V561 (≠ T534)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
32% identity, 99% coverage: 6:547/549 of query aligns to 4:564/566 of 7vzsA
- binding D-glucal: Y6 (= Y8), L22 (= L24), N25 (≠ D27), Y51 (= Y52), I349 (≠ M340), Q356 (≠ S351), E411 (≠ S397), E444 (= E430), W445 (≠ E431), K448 (≠ R434), R499 (≠ T481), N501 (≠ I483), H546 (≠ N529), K563 (≠ L546)
- binding flavin-adenine dinucleotide: G10 (= G12), G12 (= G14), T13 (= T15), S14 (≠ A16), E34 (= E36), A35 (= A37), Y51 (= Y52), F55 (≠ I56), W61 (= W63), R79 (≠ P81), G81 (= G83), G86 (= G88), T87 (≠ C89), N91 (= N93), G92 (= G94), M93 (= M95), A94 (≠ L96), T232 (≠ Q230), A233 (≠ V231), A273 (= A269), G274 (= G270), R277 (≠ G273), F502 (= F484), A536 (= A519), H546 (≠ N529), L547 (≠ T530), V548 (≠ N531), L551 (≠ T534)
Sites not aligning to the query:
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
32% identity, 99% coverage: 6:547/549 of query aligns to 4:564/569 of 4ynuA
- active site: V341 (≠ W332), F412 (≠ V398), W413 (≠ C399), N501 (≠ I483), H503 (= H485), G545 (= G528), H546 (≠ N529)
- binding flavin-adenine dinucleotide: G12 (= G14), T13 (= T15), S14 (≠ A16), E34 (= E36), A35 (= A37), Y51 (= Y52), F55 (≠ I56), W61 (= W63), R79 (≠ P81), G81 (= G83), G86 (= G88), T87 (≠ C89), N91 (= N93), G92 (= G94), T232 (≠ Q230), A233 (≠ V231), A273 (= A269), G274 (= G270), R277 (≠ G273), F502 (= F484), A536 (= A519), H546 (≠ N529), L547 (≠ T530), V548 (≠ N531), L551 (≠ T534)
- binding D-glucono-1,5-lactone: Y51 (= Y52), E411 (≠ S397), A496 (≠ D478), N497 (≠ I479), R499 (≠ T481), R499 (≠ T481), N501 (≠ I483), H503 (= H485), H546 (≠ N529)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
32% identity, 99% coverage: 6:547/549 of query aligns to 5:565/570 of 4yntA
- active site: V342 (≠ W332), F413 (≠ V398), W414 (≠ C399), N502 (≠ I483), H504 (= H485), G546 (= G528), H547 (≠ N529)
- binding dihydroflavine-adenine dinucleotide: G13 (= G14), T14 (= T15), S15 (≠ A16), E35 (= E36), A36 (= A37), F56 (≠ I56), W62 (= W63), R80 (≠ P81), G82 (= G83), G87 (= G88), T88 (≠ C89), N92 (= N93), G93 (= G94), M94 (= M95), A95 (≠ L96), A234 (≠ V231), A274 (= A269), R278 (≠ G273), F503 (= F484), A537 (= A519), H547 (≠ N529), L548 (≠ T530), V549 (≠ N531), L552 (≠ T534)
6xuuA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, glucose-bound form (see paper)
29% identity, 99% coverage: 4:545/549 of query aligns to 13:585/589 of 6xuuA
- binding beta-D-glucopyranose: E35 (≠ A26), N67 (= N57), H83 (≠ N74), N104 (≠ A102), D108 (= D106), S139 (vs. gap), A140 (vs. gap), N142 (vs. gap), T166 (≠ R156), D169 (≠ K159), T188 (≠ A177), W213 (vs. gap), A225 (= A211), Y226 (≠ F212), Q329 (= Q312), N330 (vs. gap), A331 (vs. gap), Q357 (≠ K318), F414 (≠ A383), K474 (≠ R445), N517 (≠ T481), G520 (vs. gap), A522 (vs. gap), V524 (≠ I483), H526 (= H485), H569 (≠ N529)
- binding flavin-adenine dinucleotide: G21 (= G12), G23 (= G14), L24 (≠ T15), T25 (≠ A16), E45 (= E36), A46 (= A37), F66 (≠ I56), W72 (= W63), H83 (≠ N74), G85 (= G83), G90 (= G88), S91 (≠ C89), N95 (= N93), G96 (= G94), A97 (≠ M95), A98 (≠ L96), M244 (≠ Q230), A245 (≠ V231), A286 (= A269), Q290 (≠ G273), A525 (≠ F484), A559 (= A519), H569 (≠ N529), L570 (≠ T530), S571 (≠ N531), L574 (≠ T534)
- binding alpha-D-glucopyranose: G96 (= G94), F414 (≠ A383), W428 (≠ C399)
Query Sequence
>WP_086510929.1 NCBI__GCF_002151265.1:WP_086510929.1
MVEQEFDYIVVGAGTAGCLLANRLSADPANRVLLIEAGGRDNYHWVHIPVGYLYCINNPR
TDWCFKTEPDKGLNGRSLIYPRGKTLGGCSSINGMLYLRGQARDYDGWAELVGDDDWRWE
NCLPDFMKHEDHYRLDEGGDADAAHRDYHGHGGEWRIEKQRLKWEVLDDFAEAAVQAGVP
RTRDFNRGDNEGVDYFEVNQRDGWRWNAAKAFLRPVCEKRDNFTLWHSTQVHRLLFDTAE
GKPRASGVELERNGTVSRVSAKREVILAAGAIGSPQLLQLSGIGPAALLAEHGIPLVHEL
PGVGENLQDHLQIRSVYKVTGAKTLNTLAASWLGKAKIGMEYLLRRTGPMSMAPSQLCIF
TRSSDEYEHPNIEYHVQPLSLEAFGQPLHDYPAITASVCNLNPTSRGSVRIKSRDPRQAP
AIAPNYLSTEEDRRVAADSLRVTRRIAEQPAFAKYRPEEVKPGVQYQSDEDLARLAGDIG
TTIFHPVGTAKMGRGDDPMAVVDSRLRVRGVEGLRVVDASVMPTITSGNTNSPTLMIAEK
AAAWILESR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory