SitesBLAST
Comparing WP_086510986.1 NCBI__GCF_002151265.1:WP_086510986.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q53TZ2 L-arabinose 1-dehydrogenase (NAD(P)(+)); D-galactose 1-dehydrogenase; EC 1.1.1.376; EC 1.1.1.120; EC 1.1.1.48 from Azospirillum brasilense (see paper)
53% identity, 98% coverage: 4:306/308 of query aligns to 5:307/309 of Q53TZ2
- D169 (= D168) mutation to A: Loss of activity.
- N173 (= N172) mutation to A: Decrease by 4 orders of magnitude in catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7cgqA Crystal structure of azospirillum brasilense l-arabinose 1- dehydrogenase e147a mutant (NADP and l-arabinose bound form)
53% identity, 98% coverage: 4:306/308 of query aligns to 2:304/306 of 7cgqA
- binding alpha-L-arabinopyranose: K88 (= K90), H116 (= H118), H150 (= H152), P167 (= P169), N170 (= N172)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G12), K11 (= K13), I12 (= I14), S34 (≠ D36), R35 (≠ P37), H36 (≠ R38), C64 (= C66), A65 (≠ T67), P66 (= P68), V69 (≠ L71), E87 (= E89), K88 (= K90), H116 (= H118), Q153 (= Q155), W155 (= W157), D166 (= D168), Y263 (= Y265)
3m2tA The crystal structure of dehydrogenase from chromobacterium violaceum
32% identity, 42% coverage: 1:130/308 of query aligns to 1:137/342 of 3m2tA
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), G12 (= G12), A13 (≠ K13), Q14 (≠ I14), D36 (= D36), S37 (≠ P37), R41 (= R38), A73 (≠ C66), G74 (≠ T67), L78 (= L71), E96 (= E89), K97 (= K90), N125 (≠ H118)
Sites not aligning to the query:
1zh8A Crystal structure of oxidoreductase (tm0312) from thermotoga maritima at 2.50 a resolution
24% identity, 94% coverage: 4:293/308 of query aligns to 4:304/325 of 1zh8A
- active site: K98 (= K90), H187 (≠ N172)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), C11 (≠ A11), G12 (= G12), I13 (≠ K13), A14 (≠ I14), S37 (vs. gap), R38 (vs. gap), T39 (vs. gap), H42 (vs. gap), T74 (≠ C66), L75 (≠ T67), P76 (= P68), L79 (= L71), E97 (= E89), K98 (= K90), N126 (≠ H118), Y165 (≠ V150), W170 (= W157), R171 (≠ I158), H187 (≠ N172), Y276 (= Y269)
7x2yA Crystal structure of cis-4,5-dihydrodiol phthalate dehydrogenase in complex with NAD+ and 3-hydroxybenzoate (see paper)
29% identity, 45% coverage: 4:142/308 of query aligns to 4:147/342 of 7x2yA
- binding 3-hydroxybenzoic acid: H122 (≠ W117)
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), G12 (= G12), R13 (≠ K13), A14 (≠ I14), D35 (= D36), P36 (= P37), R37 (= R38), A71 (≠ C66), S72 (≠ T67), P73 (= P68), F76 (≠ L71), Q80 (= Q75), E94 (= E89), K95 (= K90), P96 (= P91)
Sites not aligning to the query:
4nheB The crystal structure of oxidoreductase (gfo/idh/moca family) from streptococcus pneumoniae tigr4 in complex with NADP
23% identity, 59% coverage: 3:184/308 of query aligns to 2:194/326 of 4nheB
- active site: K94 (= K90), Y182 (≠ N172)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T10 (≠ A11), I13 (= I14), S34 (≠ D36), R35 (≠ P37), T39 (≠ L41), A70 (≠ C66), S71 (≠ T67), P72 (= P68), N73 (≠ T69), H76 (≠ R72), E93 (= E89), K94 (= K90), R122 (≠ H118), N165 (≠ Q155), F171 (≠ P161), Y182 (≠ N172)
7xr9A Crystal structure of dgpa with glucose (see paper)
29% identity, 34% coverage: 16:121/308 of query aligns to 17:122/344 of 7xr9A
Sites not aligning to the query:
1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
24% identity, 56% coverage: 8:181/308 of query aligns to 7:197/340 of 1evjA
- active site: K100 (= K90), Y188 (≠ N172)
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), L10 (≠ A11), G11 (= G12), K12 (= K13), Y13 (≠ I14), D35 (= D36), L77 (≠ T67), P78 (= P68), N79 (≠ T69), H82 (≠ R72), E99 (= E89), K100 (= K90), R128 (≠ H118), W170 (= W157), R171 (≠ I158), Y188 (≠ N172)
Sites not aligning to the query:
6jw6A The crystal structure of kand2 in complex with NAD (see paper)
35% identity, 41% coverage: 4:129/308 of query aligns to 2:133/341 of 6jw6A
- binding nicotinamide-adenine-dinucleotide: G10 (= G12), F11 (≠ I14), M12 (≠ V15), D33 (= D36), L34 (vs. gap), T70 (≠ C66), T71 (= T67), P72 (= P68), N73 (≠ T69), L75 (= L71), H76 (≠ R72), Q79 (= Q75), E93 (= E89), K94 (= K90), N122 (≠ H118)
Sites not aligning to the query:
6jw8A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin b (see paper)
42% identity, 25% coverage: 54:129/308 of query aligns to 58:133/342 of 6jw8A
- binding 1,4-dihydronicotinamide adenine dinucleotide: T70 (≠ C66), T71 (= T67), P72 (= P68), N73 (≠ T69), L75 (= L71), H76 (≠ R72), Q79 (= Q75), E93 (= E89), K94 (= K90), N122 (≠ H118)
Sites not aligning to the query:
- binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: 11, 152, 154, 175, 176, 179, 236, 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 10, 11, 12, 33, 34, 161, 179, 290
6jw7A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin a (see paper)
42% identity, 25% coverage: 54:129/308 of query aligns to 58:133/342 of 6jw7A
- binding 1,4-dihydronicotinamide adenine dinucleotide: T70 (≠ C66), T71 (= T67), P72 (= P68), N73 (≠ T69), L75 (= L71), H76 (≠ R72), Q79 (= Q75), E93 (= E89), K94 (= K90), N122 (≠ H118)
Sites not aligning to the query:
- binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: 11, 154, 175, 179, 236, 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 8, 10, 11, 12, 33, 34, 161, 290
2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
33% identity, 43% coverage: 7:139/308 of query aligns to 4:142/332 of 2glxA
- active site: K93 (= K90)
- binding acetate ion: K93 (= K90)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G10), A8 (= A11), S9 (≠ G12), T10 (≠ K13), I11 (= I14), S32 (≠ A33), T33 (= T34), R37 (= R38), S69 (≠ C66), T70 (= T67), N72 (≠ T69), H75 (≠ R72), E92 (= E89), K93 (= K90), H121 (= H118)
Sites not aligning to the query:
Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
33% identity, 43% coverage: 7:139/308 of query aligns to 5:143/333 of Q2I8V6
- ASTI 9:12 (≠ AGKI 11:14) binding NADP(+)
- S10 (≠ G12) mutation to G: Almost no effect.
- A13 (≠ V15) mutation to G: Can use NAD as cosubstrate as well as NADP.
- S33 (≠ A33) mutation to D: No activity.
- ST 33:34 (≠ AT 33:34) binding NADP(+)
- R38 (= R38) binding NADP(+)
- TTNELH 71:76 (≠ TPTHLR 67:72) binding NADP(+)
- EK 93:94 (= EK 89:90) binding NADP(+)
- K94 (= K90) mutation to G: Less than 1% remaining activity.
- N120 (≠ A116) binding NADP(+)
Sites not aligning to the query:
- 162:163 binding NADP(+)
- 176 D→A: Less than 1% remaining activity.
- 180 H→A: Less than 2% remaining activity.
- 206 G→I: No effect.
- 283 binding NADP(+)
1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
24% identity, 55% coverage: 8:175/308 of query aligns to 38:222/383 of 1h6dA
- active site: K131 (= K90), Y219 (≠ N172)
- binding glycerol: K131 (= K90), R202 (≠ I158), D215 (= D168), Y219 (≠ N172)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G10), L41 (≠ A11), G42 (= G12), K43 (= K13), Y44 (≠ I14), S66 (≠ D36), G67 (≠ P37), K71 (≠ L41), Y89 (= Y47), I107 (≠ C66), L108 (≠ T67), P109 (= P68), N110 (≠ T69), H113 (≠ R72), E130 (= E89), K131 (= K90), R159 (≠ H118), A198 (≠ G154), W201 (= W157), R202 (≠ I158), Y219 (≠ N172)
Sites not aligning to the query:
1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
24% identity, 55% coverage: 8:175/308 of query aligns to 36:220/381 of 1rydA
- active site: K129 (= K90), Y217 (≠ N172)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (≠ A11), G40 (= G12), K41 (= K13), Y42 (≠ I14), S64 (≠ D36), G65 (≠ P37), K69 (≠ L41), Y87 (= Y47), L106 (≠ T67), P107 (= P68), N108 (≠ T69), L110 (= L71), H111 (≠ R72), E128 (= E89), K129 (= K90), R157 (≠ H118), A196 (≠ G154), W199 (= W157), R200 (≠ I158), Y217 (≠ N172)
Sites not aligning to the query:
9azoA Crystal structure of chms dehydrogenase pmdc from comamonas testosteroni bound to cofactor NADP (see paper)
26% identity, 48% coverage: 4:151/308 of query aligns to 3:152/315 of 9azoA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), G11 (= G12), A12 (≠ K13), F13 (≠ I14), G34 (vs. gap), R35 (vs. gap), C71 (= C66), T72 (= T67), T74 (= T69), H77 (≠ R72), E94 (= E89), I95 (≠ K90)
Sites not aligning to the query:
2poqX Dimeric dihydrodiol dehydrogenase complexed with inhibitor, isoascorbic acid (see paper)
25% identity, 58% coverage: 3:181/308 of query aligns to 6:188/331 of 2poqX
Sites not aligning to the query:
2o4uX Crystal structure of mammalian dimeric dihydrodiol dehydrogenase (see paper)
25% identity, 58% coverage: 3:181/308 of query aligns to 6:188/331 of 2o4uX
- binding phosphate ion: S8 (≠ T5), V9 (≠ L6), A35 (≠ S26), R36 (= R27), R40 (≠ T34), Y57 (≠ T51), N101 (≠ T95), A103 (≠ S97), D145 (≠ R139), R147 (≠ E141), K155 (≠ V148)
Sites not aligning to the query:
- binding phosphate ion: 1, 2, 232, 234, 236, 247, 250, 253, 292, 296, 318
Q7JK39 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; D-xylose 1-dehydrogenase; D-xylose-NADP dehydrogenase; Dimeric dihydrodiol dehydrogenase; Jmo2DD; EC 1.3.1.20; EC 1.1.1.179 from Macaca fuscata fuscata (Japanese macaque) (see paper)
25% identity, 58% coverage: 3:181/308 of query aligns to 7:189/334 of Q7JK39
- H79 (≠ R72) mutation to E: Decrease in K(d) and K(m) value for NADPH. Elimination of the fluorescence-energy transfer and enhancement of NADPH fluorescence by the binary complex formation. Potent inhibition of the dehydrogenase activity by high ionic strength.
- Y180 (≠ N172) mutation to F: Significant loss of activity. No effect on the high affinity for NADPH, fluorescence-energy transfer and enhancement of NADPH fluorescence by the binary complex formation.
Q9TQS6 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; Cmo2DD; D-xylose 1-dehydrogenase; D-xylose-NADP dehydrogenase; Dimeric dihydrodiol dehydrogenase; EC 1.3.1.20; EC 1.1.1.179 from Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) (see paper)
25% identity, 58% coverage: 3:181/308 of query aligns to 7:189/334 of Q9TQS6
- R148 (≠ E141) mutation to A: No effect on activity. Reduced activity and exhibits significant temperature sensitivity; when associated with A-202.
Sites not aligning to the query:
- 202 R→A: No effect on activity. Reduced activity and exhibits significant temperature sensitivity; when associated with A-148.
Query Sequence
>WP_086510986.1 NCBI__GCF_002151265.1:WP_086510986.1
MTVITLGLVGAGKIVRDQHLPALAESRDFRLLATADPRARLASMPGYASLTEMLEAHPEL
EAVAICTPTHLRYSQARIALERGRSVLLEKPPGATLSEVEELVELAERHGGTLFAAWHSR
FAPAVAEARDWLRERRIQRVEIDWREDVRVWHPGQAWIWEPAGMGVFDPGINALSIATTI
LPRAFFLRDARLWIPSNCQAPIAAALTFSDAEGTPIVADFDFRQPSPPIWSIHVETEAGR
LSLLRGGCRLELDGRCLLDAEEREYPNLYARFAELIRQRRSDVDLAPLRHVADACLSAWR
EPTEPFIE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory