SitesBLAST

E205 (≠ D204) mutation to A: Has no effect on the growth rate.
E208 (= E207) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (≠ E212) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

7cagA Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
30% identity, 87% coverage: 33:302/310 of query aligns to 13:277/285 of 7cagA

query
sites
7cagA

L

L

L

I

P

A

P

P

A

A

L

V

I

L

L

L

F

M

S

L

L

A

F

V

V

T

I

A

L

Y

P

P

L

I

A

G

D

Y

A

A

A

V

Y

W

Y

L

S

S

A

L

F

Q

S

R

W

Y

N

N

-

L

G

A

Y

E

G

P

T

H

P

D

T

T

N

E

F

F

V

I

G

G

T

L

Q

A

N

N

Y

Y

E

V

R

T

L

V

L

L

D

T

H

D

S

G

V

Y

F

W

H

W

T

T

A

A

L

F

W

A

N

V

T

T

A

L

K

G

L

I

I

T

L

V

V

V

S

S

L

V

I

A

I

I

Q

E

M

F

P

A

L

L

A

G

L

L

G

A

L

L

A

A

L

L

L

V

V

M

Y

H

R

R

K

T

T

I

P

F

T

G

N

K

T

G

L

A

F

V

R

R

L

T

V

A

F

I

F

L

L

I

P

P

Y

Y

I

G

L

I

A

V

E

T

V

V

A

A

G

S

L

Y

I

S

W

W

S

Y

F

Y

V

A

F

W

D

T

G

P

D

G

Y

T

G

G

I

Y

T

L

A

A

F

-

M

-

F

-

Q

-

T

N

L

L

G

L

Q

P

E

E

S

G

V

S

Y

A

V

P

L

L

A

T

D

D

R

Q

Q

L

W

P

A

S

F

L

P

A

A

I

I

V

M

V

T

L

V

A

I
x
E

V

V

W

W

K
|
K

Y

T

F

T

G

P

F

F

H

M

M

A

M

L

I

L

Y

L

I

L

A

A

A

G

L

L

Q

A

S

L

V

V

P

P

K

Q

D

D

L

L

I

L

E

N

A

A

K

Q

L

V

E

D

G

G

A

A

K

G

P

P

R

W

Q

K

V

R

A

L

L

T

F

K

V

V

Q

I

I

L

P

P

L

M

I

I

K

K

H

P

A

A

I

I

V

L

V

V

S

A

G

L

F

L

F

F

A
x
R

I

T

I

L

G
x
D

S

A

L

F

Q

R

I

I

F

F

D

D

I

N

I

I

I

Y

P

I

M

L

T

T

N

G

G

G

G

-

P

-

S

S

N

N

S

D

T

T

H

G

T

S

I

V

V

S

T

I

Y

L

L

G

Y

Y

T

D

F

N

G

L

L

F

S

K

R

A

L

F

N

N

I

V

G

G

F

L

G

G

S

S

A

A

A

I

A

S

V

V

V

L

L

I

F

F

V

L

L

S

A

V

I

A

G

I

I

I

A

A

L

F

F

I

Y

Y

Q

I

R

K

binding alpha-D-glucopyranose: E159 (≠ I182), K162 (= K185), R216 (≠ A239), D219 (≠ G242)

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
24% identity, 67% coverage: 96:304/310 of query aligns to 289:509/514 of P02916

query
sites
P02916

L

L

V

I

S

T

L

V

I

F

I

L

Q

T

M

V

P

A

L

V

A

G

L

M

G

V

L

L

A

A

L

C

L

L

V

V

-

Q

Y

W

R

E

K

A

T

L

P

R

T

G

N

K

T

A

L

V

F

Y

R

R

L

V

V

L

F

L

F

I

L

L

P

P

Y

Y

I

A

L

V

A

P

E

S

V

F

A

I

A

S

G

I

L
|
L

I

I

W

F

S

K

F

G

V

L

F

F

D

N

G

Q

D

S

Y

F

G

G

I

E

T

I

A

N

F

M

M

M

F

L

Q

S

T

A

L

L

G

F

Q

G

E

V

S

K

V

P

Y

A

V

W

L

F

A

S

D

D

R

P

Q

T

W

T

A

A

F

R

P

T

A

M

I
x
L

M

I

T

I

V

V

I
x
N

V

T

W

W

K

L

Y
x
G

F

Y

G

P

F

Y

H

M

M

M

M

I

I

L

Y

C

I

M

A

G

A

L

L

L

Q

K

S

A

V

I

P

P

K

D

D

D

L

L

I

Y

E
|
E

A

A

A
x
S

K

A

L

M

E

D

G
|
G

A

A

K

G

P

P

R

F

Q

Q

V

N

A

F

L

F

F

K

V

I

Q

T

I

L

P
|
P

L

L

I

L

K

I

H

K

A

P

I

L

V

T

V

P

S

L

G

M

F

I

F

A

A

S

I

F

I

A

G

F

S

N

L

F

Q

N

I

N

F

F

D

V

I

L

I

I

I

Q

P

L

M

L

T

T

N

N

G

G

-

P

-

D

-

R

-

L

-

G

-

T

P

P

S

A

N

G

S

Y

T

T

H

D

T

L

I

L

V

V

T

N

Y

Y

L

T

Y

Y

T

R

F

I

-

A

-

F

-

E

G

G

L

G

S

G

R

G

L

Q

N

D

I

F

G

G

F

L

G

A

S

A

A

A

A

I

A

A

V

T

V

L

L

I

F

F

V

L

L

L

A

V

I

G

G

A

I

L

A

A

L

I

F

V

Y

N

Q

L

R

K

A

A

T

T

L334 (= L140) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ I178) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ I182) mutation N->K,H: No growth on maltose.
G380 (≠ Y186) mutation G->C,S: No growth on maltose.
E401 (= E207) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A209) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G213) mutation G->A,P: No effect.
P420 (= P226) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
22% identity, 81% coverage: 48:298/310 of query aligns to 219:488/490 of 4ki0F

query
sites
4ki0F

P

P

L

N

A

N

D

Q

A

I

A

G

Y

F

Y

Y

S

Q

A

S

F

I

S

N

W

W

N

G

-

D

-

E

-

K

-

L

-

S

-

P

G

G

Y

Y

G

T

T

V

P

T

T

T

N

-

F

-

V

-

G

G

T

W

Q

K

N

N

Y

F

E

T

R

R

L

V

L

F

D

T

H

D

S

E

V

G

F

I

H

Q

T

K

A

P

-

F

-

L

-

A

-

I

-

F

L

V

W

W

N

-

T

T

A

V

K

V

L

F

I

S

L

L

V

I

S

T

L

V

I

F

I

L

Q

T

M

V

P

A

L

V

A

G

L

M

G

V

L

L

A

A

L

C

L

L

V

V

-

Q

Y

W

R

E

K

A

T

L

P

R

T

G

N

K

T

A

L

V

F

Y

R

R

L

V

V

L

F

L

F

I

L

L

P

P

Y
|
Y

I

A

L

V

A

P

E

S

V

F

A

I

A

S

G

I

L

L

I

I

W

F

S

K

F

G

V

L

F

F

D

N

G

Q

D

S

Y

F

G

G

I

E

T

I

A

N

F

M

M

M

F

L

Q

S

T

A

L

L

G

F

Q

G

E

V

S

K

V

P

Y

A

V

W

L

F

A

S

D

D

R

P

Q

T

W

T

A

A

F

R

P

T

A

M

I

L

M

I

T

I

V

V

I
x
N

V

T

W

W

K

L

Y
x
G

F

Y

G

P

F
x
Y

H

M

M

M

M

I

I

L

Y

C

I

M

A

G

A

L

L

L

Q

K

S

A

V

I

P

P

K

D

D

D

L

L

I

Y

E

E

A

A

A

S

K

A

L

M

E

D

G

G

A

A

K

G

P

P

R

F

Q

Q

V

N

A

F

L

F

F

K

V

I

Q

T

I

L

P

P

L

L

I

L

K

I

H

K

A

P

I

L

V

T

V

P

S

L

G

M

F

I

F

A

A
x
S

I

F

I

A

G

F

S
x
N

L

F

Q

N

I

N

F

F

D

V

I

L

I

I

I

Q

P

L

M

L

T

T

N

N

G

G

-

P

-

D

-

R

-

L

-

G

-

T

P

P

S

A

N

G

S

Y

T

T

H

D

T

L

I

L

V

V

T

N

Y

Y

L

T

Y

Y

T

R

F

I

-

A

-

F

-

E

G

G

L

G

S

G

R

G

L

Q

N

D

I

F

G

G

F

L

G

A

S

A

A

A

A

I

A

A

V

T

V

L

L

I

F

F

V

L

L

L

A

V

I

G

G

A

I

L

A

A

L

I

binding alpha-D-glucopyranose: Y310 (= Y131), N361 (≠ I182), G365 (≠ Y186), Y368 (≠ F189), S418 (≠ A239), N422 (≠ S243)

Query Sequence

>WP_086511009.1 NCBI__GCF_002151265.1:WP_086511009.1
MSTLTRPAVHRRSNTDRLRQGLANGKFTAVLILLPPALILFSLFVILPLADAAYYSAFSW
NGYGTPTNFVGTQNYERLLDHSVFHTALWNTAKLILVSLIIQMPLALGLALLVYRKTPTN
TLFRLVFFLPYILAEVAAGLIWSFVFDGDYGITAFMFQTLGQESVYVLADRQWAFPAIMT
VIVWKYFGFHMMIYIAALQSVPKDLIEAAKLEGAKPRQVALFVQIPLIKHAIVVSGFFAI
IGSLQIFDIIIPMTNGGPSNSTHTIVTYLYTFGLSRLNIGFGSAAAVVLFVLAIGIALFY
QRATAKEERT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory