SitesBLAST
Comparing WP_086511037.1 NCBI__GCF_002151265.1:WP_086511037.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6ciaA Crystal structure of aldo-keto reductase from klebsiella pneumoniae in complex with NADPH.
60% identity, 93% coverage: 22:296/296 of query aligns to 14:284/284 of 6ciaA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G28), T21 (= T29), W22 (= W30), D50 (= D58), Y55 (= Y63), H113 (= H121), N143 (= N151), Q165 (= Q173), Y193 (= Y201), C194 (= C202), P195 (= P203), L196 (= L204), Q198 (= Q206), A199 (= A207), A224 (= A232), I240 (= I252), P241 (= P253), K242 (= K254), A243 (= A255), A244 (≠ V256), S245 (≠ Q257), H248 (= H260), N252 (= N264)
4wghA Crystal structure of aldo/keto reductase from klebsiella pneumoniae in complex with NADP and acetate at 1.8 a resolution
60% identity, 93% coverage: 22:296/296 of query aligns to 13:283/283 of 4wghA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G28), T20 (= T29), W21 (= W30), D49 (= D58), Y54 (= Y63), N142 (= N151), Q164 (= Q173), Y192 (= Y201), C193 (= C202), P194 (= P203), L195 (= L204), Q197 (= Q206), A198 (= A207), R200 (≠ Q209), A223 (= A232), I239 (= I252), P240 (= P253), K241 (= K254), A242 (= A255), A243 (≠ V256), S244 (≠ Q257), H247 (= H260), N251 (= N264)
5c7hA Crystal structure of aldo-keto reductase from sinorhizobium meliloti 1021 in complex with NADPH
52% identity, 98% coverage: 8:296/296 of query aligns to 2:281/281 of 5c7hA
- active site: D52 (= D58), Y57 (= Y63), K82 (= K88), H115 (= H121)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G22 (= G28), T23 (= T29), W24 (= W30), D52 (= D58), Y57 (= Y63), H115 (= H121), Q167 (= Q173), Y195 (= Y201), S196 (≠ C202), P197 (= P203), L198 (= L204), E200 (≠ Q206), L204 (≠ R211), A221 (= A232), I237 (= I252), P238 (= P253), K239 (= K254), T240 (≠ A255), R245 (≠ H260), E248 (= E263), N249 (= N264), I281 (≠ V296)
4pmjA Crystal structure of a putative oxidoreductase from sinorhizobium meliloti 1021 in complex with NADP
52% identity, 98% coverage: 8:296/296 of query aligns to 2:281/281 of 4pmjA
- active site: D52 (= D58), Y57 (= Y63), K82 (= K88), H115 (= H121)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G22 (= G28), T23 (= T29), W24 (= W30), D52 (= D58), H115 (= H121), Q167 (= Q173), Y195 (= Y201), S196 (≠ C202), P197 (= P203), L198 (= L204), E200 (≠ Q206), L204 (≠ R211), A221 (= A232), I237 (= I252), P238 (= P253), K239 (= K254), T240 (≠ A255), G241 (≠ V256), R245 (≠ H260), E248 (= E263), N249 (= N264)
5danA Crystal structure of a novel aldo keto reductase tm1743 from thermotoga maritima in complex with NADP+
40% identity, 89% coverage: 21:282/296 of query aligns to 12:272/274 of 5danA
- active site: D53 (= D58), Y58 (= Y63), K84 (= K88), H117 (= H121)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G28), T20 (= T29), W21 (= W30), D53 (= D58), Y58 (= Y63), H117 (= H121), Q169 (= Q173), Y198 (= Y201), S199 (≠ C202), P200 (= P203), L201 (= L210), R203 (= R212), Y226 (≠ A232), I242 (= I252), P243 (= P253), K244 (= K254), G246 (≠ V256), R247 (≠ Q257), H250 (= H260), E253 (= E263), N254 (= N264)
6kiyA Crystal structure of a thermostable aldo-keto reductase tm1743 in complex with inhibitor epalrestat (see paper)
40% identity, 89% coverage: 21:282/296 of query aligns to 13:273/275 of 6kiyA
- binding {5-[(2E)-2-methyl-3-phenylprop-2-en-1-ylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl}acetic acid: W22 (= W30), Y59 (= Y63), W87 (≠ Y90), H118 (= H121), R204 (= R212)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G28), T21 (= T29), W22 (= W30), Y59 (= Y63), H118 (= H121), N149 (= N151), Q170 (= Q173), Y199 (= Y201), S200 (≠ C202), P201 (= P203), L202 (= L210), R204 (= R212), T205 (≠ D213), Y227 (≠ A232), I243 (= I252), P244 (= P253), K245 (= K254), G247 (≠ V256), R248 (≠ Q257), H251 (= H260), E254 (= E263), N255 (= N264)
6kikA Crystal structure of a thermostable aldo-keto reductase tm1743 in complex with inhibitor tolrestat (see paper)
40% identity, 89% coverage: 21:282/296 of query aligns to 13:273/275 of 6kikA
A0QV10 Aldo-keto reductase MSMEG_2408/MSMEI_2347; EC 1.1.1.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
35% identity, 88% coverage: 22:280/296 of query aligns to 13:255/275 of A0QV10
Sites not aligning to the query:
- 262 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
1vbjA The crystal structure of prostaglandin f synthase from trypanosoma brucei
30% identity, 88% coverage: 22:281/296 of query aligns to 20:262/281 of 1vbjA
- active site: D52 (= D58), Y57 (= Y63), K82 (= K88), H115 (= H121)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G26 (= G28), M27 (≠ T29), W28 (= W30), D52 (= D58), Y57 (= Y63), H115 (= H121), N145 (= N151), Q166 (= Q173), W192 (≠ Y201), S193 (≠ C202), P194 (= P203), L195 (= L204), Q197 (= Q206), G198 (≠ A207), V201 (≠ F215), A218 (= A232), I233 (= I252), K235 (= K254), S236 (≠ A255), G237 (≠ V256), R241 (≠ H260), E244 (= E263), N245 (= N264)
Q9GV41 9,11-endoperoxide prostaglandin H2 reductase; Prostaglandin F2-alpha synthase; EC 1.1.1.- from Trypanosoma brucei brucei
30% identity, 88% coverage: 22:281/296 of query aligns to 15:257/276 of Q9GV41
P80874 Aldo-keto reductase YhdN; AKR11B; General stress protein 69; GSP69; EC 1.1.1.- from Bacillus subtilis (strain 168) (see paper)
35% identity, 63% coverage: 20:206/296 of query aligns to 11:208/331 of P80874
Sites not aligning to the query:
- 214 binding NADP(+)
- 227 binding NADP(+)
- 280:282 binding NADP(+)
- 286 binding NADP(+)
4gieA Crystal structure of prostaglandin f synthase from trypanosoma cruzi bound to NADP (see paper)
27% identity, 89% coverage: 19:281/296 of query aligns to 20:268/288 of 4gieA
- active site: D55 (= D58), Y60 (= Y63), K85 (= K88), H118 (= H121)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G29 (= G28), W31 (= W30), D55 (= D58), Y60 (= Y63), H118 (= H121), W119 (= W122), N148 (= N151), Q169 (= Q173), W195 (≠ Y201), S196 (≠ C202), P197 (= P203), L198 (= L204), S200 (≠ Q206), L207 (≠ F215), A224 (= A232), I239 (= I252), P240 (= P253), K241 (= K254), S242 (≠ A255), R247 (≠ H260), E250 (= E263), N251 (= N264)
4fziA Crystal structure of prostaglandin f synthase from trypanosoma cruzi (see paper)
27% identity, 89% coverage: 19:281/296 of query aligns to 9:257/277 of 4fziA
3d3fA Crystal structure of yvgn and cofactor NADPH from bacillus subtilis (see paper)
33% identity, 89% coverage: 20:282/296 of query aligns to 14:259/275 of 3d3fA
- active site: D48 (= D58), Y53 (= Y63), K78 (= K88), H111 (= H121)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G22 (= G28), F24 (≠ W30), D48 (= D58), Y53 (= Y63), H111 (= H121), S140 (= S150), N141 (= N151), Q162 (= Q173), W188 (≠ Y201), S189 (≠ C202), P190 (= P203), L191 (= L204), Q193 (= Q206), L197 (≠ F215), I229 (= I252), K231 (= K254), S232 (≠ A255), K234 (≠ Q257), R237 (≠ H260), E240 (= E263), N241 (= N264)
1pz1A Structure of NADPH-dependent family 11 aldo-keto reductase akr11b(holo) (see paper)
35% identity, 63% coverage: 20:206/296 of query aligns to 11:208/333 of 1pz1A
- active site: D52 (= D58), Y57 (= Y63), K90 (vs. gap), Q93 (vs. gap), H125 (= H121)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G28), W21 (= W30), Q175 (= Q173), Y203 (= Y201), G204 (≠ C202), L206 (= L204), R208 (≠ Q206)
Sites not aligning to the query:
A0QV09 Aldo-keto reductase MSMEG_2407/MSMEI_2346; AKR; AKR5H1; EC 1.1.1.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 88% coverage: 22:281/296 of query aligns to 22:265/283 of A0QV09
- G196 (≠ C202) binding NADPH
- L198 (= L204) binding NADPH
- V200 (≠ Q206) binding NADPH
- I236 (= I252) binding NADPH
- R238 (≠ K254) binding NADPH
- S239 (≠ A255) binding NADPH
- A240 (≠ V256) binding NADPH
- R244 (≠ H260) binding NADPH
- S247 (≠ E263) binding NADPH
- N248 (= N264) binding NADPH
Sites not aligning to the query:
2wzmA Crystal structure of a mycobacterium aldo-keto reductase in its apo and liganded form (see paper)
32% identity, 88% coverage: 22:281/296 of query aligns to 13:256/274 of 2wzmA
- active site: D44 (= D58), Y49 (= Y63), K74 (= K88), H107 (= H121)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: Y186 (= Y201), G187 (≠ C202), P188 (= P203), L189 (= L204), G190 (≠ A205), V191 (≠ Q206), G192 (≠ A207), L195 (≠ F215), A212 (= A232), I227 (= I252), R229 (≠ K254), S230 (≠ A255), R235 (≠ H260), N239 (= N264)
Sites not aligning to the query:
3b3dA B.Subtilis ytbe (see paper)
33% identity, 94% coverage: 6:282/296 of query aligns to 2:264/280 of 3b3dA
3v0sA Crystal structure of perakine reductase, founder member of a novel akr subfamily with unique conformational changes during NADPH binding (see paper)
27% identity, 90% coverage: 19:285/296 of query aligns to 10:278/287 of 3v0sA
- active site: D45 (= D58), Y50 (= Y63), G87 (≠ A99), H119 (= H121)
- binding 2'-monophosphoadenosine-5'-diphosphate: S198 (≠ C202), P199 (= P203), I200 (≠ L204), G201 (≠ A205), L204 (≠ G208), P246 (= P253), G247 (≠ K254), T248 (≠ A255), T249 (≠ V256), N253 (≠ H260), N256 (≠ E263), N257 (= N264)
3fx4A Porcine aldehyde reductase in ternary complex with inhibitor (see paper)
28% identity, 93% coverage: 21:294/296 of query aligns to 13:313/325 of 3fx4A
- active site: D45 (= D58), Y50 (= Y63), K80 (= K88), H113 (= H121)
- binding [(5Z)-5-{[3-(carboxymethoxy)-4-methoxyphenyl]methylidene}-2,4-dioxo-1,3-thiazolidin-3-yl]acetic acid: W22 (= W30), Y50 (= Y63), H113 (= H121), R218 (≠ Q209), A219 (≠ L210), F298 (= F284), I299 (≠ P285), V300 (≠ A286)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G28), T21 (= T29), W22 (= W30), D45 (= D58), Y50 (= Y63), H113 (= H121), Q184 (≠ G166), Y210 (= Y201), S211 (≠ C202), P212 (= P203), L213 (= L204), S215 (≠ Q206), A246 (= A232), I261 (= I252), P262 (= P253), K263 (= K254), S264 (≠ A255), V265 (= V256), T266 (≠ Q257), R269 (≠ H260), Q272 (≠ E263), N273 (= N264)
Query Sequence
>WP_086511037.1 NCBI__GCF_002151265.1:WP_086511037.1
MTDHPTPHVTLTATAHAPALELPAIGQGTWYMGEGLAPRSDEVRALQQGLELGLTLIDTA
EMYADGGAEEVVGEALAGRRDQAFLVSKVYPWNAGRDSAIDACERSLRRLGTDHLDLYLL
HWPGSIPLAETLEAFERLREQGKIRRFGVSNFDVEELDSLVALPGGGECAVNQVLYHLGS
RGIEHALRPRMQRLGMPLMAYCPLAQAGQLRRDLFEHSAVREVADGLGITPAQLLLAWAI
RPLNGRRDVIAIPKAVQPQHVAENTAALEVELSDEALARLDAAFPAPGRKVPLDIV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory