SitesBLAST
Comparing WP_086511190.1 NCBI__GCF_002151265.1:WP_086511190.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
29% identity, 56% coverage: 167:399/413 of query aligns to 118:349/369 of S5FMM4
- S202 (≠ L251) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ F382) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L392) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- 81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
29% identity, 57% coverage: 178:412/413 of query aligns to 133:367/368 of 4yshB
- active site: I262 (≠ T310), L283 (vs. gap), G305 (= G350), N335 (≠ Q380), L338 (≠ T383)
- binding flavin-adenine dinucleotide: V178 (= V223), S206 (≠ L251), W209 (= W254), R307 (= R352), H332 (= H377), R334 (≠ H379), N335 (≠ Q380), G336 (= G381), I337 (≠ F382), L338 (≠ T383)
- binding glycine: G249 (= G296), Y251 (≠ L298), Y251 (≠ L298), A264 (= A312), R307 (= R352), R334 (≠ H379), R334 (≠ H379)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
29% identity, 57% coverage: 178:412/413 of query aligns to 133:367/370 of 4yshA
- active site: I262 (≠ T310), L283 (vs. gap), G305 (= G350), N335 (≠ Q380), L338 (≠ T383)
- binding flavin-adenine dinucleotide: V178 (= V223), S206 (≠ L251), G207 (= G252), W209 (= W254), R307 (= R352), H332 (= H377), R334 (≠ H379), N335 (≠ Q380), G336 (= G381), I337 (≠ F382)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
30% identity, 46% coverage: 223:412/413 of query aligns to 180:369/377 of Q5L2C2
- V180 (= V223) binding FAD
- R309 (= R352) binding substrate
- 334:340 (vs. 377:383, 29% identical) binding FAD
- R336 (≠ H379) binding substrate
Sites not aligning to the query:
- 14:15 binding FAD
- 34:35 binding FAD
- 42:43 binding FAD
- 47:49 binding FAD
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
22% identity, 96% coverage: 5:400/413 of query aligns to 4:348/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ L8), G8 (= G9), G10 (= G11), V11 (≠ M12), I12 (≠ V13), V30 (= V31), E31 (≠ D32), K32 (≠ R33), E38 (= E39), A39 (≠ T40), S40 (= S41), A43 (≠ N44), G45 (= G46), L46 (≠ I47), V171 (= V223), G200 (≠ L251), G201 (= G252), W203 (= W254), G298 (= G350), R300 (= R352), P301 (= P353), Y326 (≠ G378), R327 (≠ H379), N328 (≠ Q380), G329 (= G381), I330 (≠ F382)
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
27% identity, 58% coverage: 172:412/413 of query aligns to 123:363/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (≠ D225), V174 (= V226), S202 (≠ L251), G203 (= G252), W205 (= W254), F209 (≠ L258), G300 (= G350), R302 (= R352), H327 (= H377), F328 (≠ G378), R329 (≠ H379), N330 (≠ Q380), G331 (= G381), I332 (≠ F382)
- binding glycolic acid: Y246 (≠ L298), R302 (= R352), R329 (≠ H379)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
27% identity, 58% coverage: 172:412/413 of query aligns to 123:363/369 of O31616
- V174 (= V226) binding FAD
- H244 (≠ K295) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (= R352) binding substrate
- 327:333 (vs. 377:383, 29% identical) binding FAD
- R329 (≠ H379) binding substrate
Sites not aligning to the query:
- 14:15 binding FAD
- 34:35 binding FAD
- 42:43 binding FAD
- 47:49 binding FAD
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
27% identity, 58% coverage: 172:412/413 of query aligns to 123:363/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ F297), R302 (= R352), R329 (≠ H379)
- binding flavin-adenine dinucleotide: V174 (= V226), S202 (≠ L251), G203 (= G252), W205 (= W254), F209 (≠ L258), G300 (= G350), R302 (= R352), H327 (= H377), R329 (≠ H379), N330 (≠ Q380), G331 (= G381), I332 (≠ F382)
- binding phosphate ion: R254 (= R307)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
6pxsA Crystal structure of iminodiacetate oxidase (idaa) from chelativorans sp. Bnc1 (see paper)
25% identity, 97% coverage: 4:405/413 of query aligns to 2:360/370 of 6pxsA
- binding flavin-adenine dinucleotide: G7 (= G9), G9 (= G11), I10 (≠ M12), D30 (= D32), N32 (≠ R34), H33 (≠ E35), K36 (≠ E39), A37 (≠ T40), T38 (≠ S41), A40 (≠ G43), G41 (≠ N44), A42 (= A45), G43 (= G46), V44 (≠ I47), Y174 (≠ V223), A203 (≠ L251), W206 (= W254), I210 (≠ L258), Y250 (≠ F297), G305 (= G350), R307 (= R352), G333 (= G378), A334 (≠ H379), S335 (≠ Q380), G336 (= G381), L337 (≠ F382), T338 (= T383)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
25% identity, 51% coverage: 204:413/413 of query aligns to 189:383/383 of 5i39A
- active site: Q248 (≠ H275), P267 (≠ E294)
- binding flavin-adenine dinucleotide: A196 (= A212), A197 (= A213), G226 (≠ L251), G227 (= G252), W229 (= W254), Q248 (≠ H275), Q250 (≠ H277), G321 (= G350), M323 (≠ R352), T348 (≠ H377), G349 (= G378), W350 (≠ H379), G351 (= G381), M352 (≠ F382), T353 (= T383)
Sites not aligning to the query:
- active site: 66, 69, 70
- binding flavin-adenine dinucleotide: 30, 31, 33, 34, 35, 53, 54, 55, 62, 63, 66, 67, 69
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
22% identity, 99% coverage: 4:413/413 of query aligns to 27:400/824 of Q8GAI3
- W66 (≠ F42) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (≠ G43) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
7rdfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f co-crystallized in the presence of d-arginine (see paper)
26% identity, 55% coverage: 169:396/413 of query aligns to 116:349/375 of 7rdfA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S})-5-[5-[(~{E})-4-carbamimidamidobut-2-enoyl]-7,8-dimethyl-2,4-bis(oxidanylidene)-1~{H}-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: E170 (≠ S222), A171 (≠ V223), A199 (≠ L251), G200 (= G252), W202 (= W254), R222 (≠ G273), F249 (≠ L298), G303 (= G350), R305 (= R352), Q330 (≠ H377), G331 (= G378), G332 (≠ H379), Y333 (≠ Q380), G334 (= G381), I335 (≠ F382), Q336 (≠ T383)
- binding 6-hydroxy-flavin-adenine dinucleotide: E170 (≠ S222), A171 (≠ V223), A199 (≠ L251), G200 (= G252), W202 (= W254), R222 (≠ G273), G303 (= G350), R305 (= R352), Q330 (≠ H377), G331 (= G378), G332 (≠ H379), Y333 (≠ Q380), G334 (= G381), I335 (≠ F382), Q336 (≠ T383)
Sites not aligning to the query:
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S})-5-[5-[(~{E})-4-carbamimidamidobut-2-enoyl]-7,8-dimethyl-2,4-bis(oxidanylidene)-1~{H}-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: 9, 10, 12, 13, 14, 31, 32, 33, 40, 41, 42, 44, 45, 46, 48, 53, 87
- binding 6-hydroxy-flavin-adenine dinucleotide: 9, 10, 12, 13, 14, 32, 33, 40, 41, 42, 45, 46, 48
6pldA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with 6-oh-fad - green fraction (see paper)
26% identity, 55% coverage: 169:396/413 of query aligns to 116:349/375 of 6pldA
- binding 6-hydroxy-flavin-adenine dinucleotide: E170 (≠ S222), A171 (≠ V223), A199 (≠ L251), G200 (= G252), W202 (= W254), G303 (= G350), R305 (= R352), Q330 (≠ H377), G331 (= G378), G332 (≠ H379), Y333 (≠ Q380), G334 (= G381), I335 (≠ F382), Q336 (≠ T383)
Sites not aligning to the query:
- binding 6-hydroxy-flavin-adenine dinucleotide: 9, 10, 12, 13, 14, 31, 32, 33, 40, 41, 42, 44, 45, 46, 48
6p9dA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with fad - yellow fraction (see paper)
26% identity, 55% coverage: 169:396/413 of query aligns to 116:349/375 of 6p9dA
- binding dihydroflavine-adenine dinucleotide: E170 (≠ S222), A171 (≠ V223), A199 (≠ L251), G200 (= G252), W202 (= W254), G303 (= G350), R305 (= R352), Q330 (≠ H377), G331 (= G378), G332 (≠ H379), Y333 (≠ Q380), G334 (= G381), I335 (≠ F382), Q336 (≠ T383)
Sites not aligning to the query:
- binding dihydroflavine-adenine dinucleotide: 9, 10, 12, 13, 14, 32, 33, 40, 41, 42, 44, 45, 46, 47, 48
Q9HXE3 FAD-dependent catabolic D-arginine dehydrogenase DauA; D-arginine dehydrogenase; DADH; D-arginine utilization protein A; Dau; EC 1.4.99.6 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
26% identity, 55% coverage: 169:396/413 of query aligns to 116:349/375 of Q9HXE3
Sites not aligning to the query:
- 14 binding FAD
- 32:33 binding FAD
- 41:48 binding FAD
- 87 Important for specificity toward positively charged substrates
3sm8A Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with an (n5) flavin adduct (see paper)
26% identity, 55% coverage: 169:396/413 of query aligns to 122:355/381 of 3sm8A
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[7,8-dimethyl-5-(3-methylbutanoyl)-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: E176 (≠ S222), A177 (≠ V223), A205 (≠ L251), G206 (= G252), W208 (= W254), I212 (≠ L258), R228 (≠ G273), M246 (= M291), Y255 (≠ L298), G309 (= G350), R311 (= R352), Q336 (≠ H377), G337 (= G378), G338 (≠ H379), Y339 (≠ Q380), G340 (= G381), I341 (≠ F382), Q342 (≠ T383)
Sites not aligning to the query:
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[7,8-dimethyl-5-(3-methylbutanoyl)-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: 15, 16, 18, 19, 20, 37, 38, 39, 46, 47, 48, 50, 51, 52, 54, 59
3nyfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with imino-histidine (see paper)
26% identity, 55% coverage: 169:396/413 of query aligns to 122:355/381 of 3nyfA
- binding flavin-adenine dinucleotide: E176 (≠ S222), A177 (≠ V223), A205 (≠ L251), G206 (= G252), W208 (= W254), I212 (≠ L258), G309 (= G350), R311 (= R352), Q336 (≠ H377), G337 (= G378), G338 (≠ H379), Y339 (≠ Q380), G340 (= G381), I341 (≠ F382), Q342 (≠ T383)
- binding (2Z)-3-(1H-imidazol-5-yl)-2-iminopropanoic acid: R228 (≠ G273), M246 (= M291), Y255 (≠ L298), R311 (= R352), G338 (≠ H379)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 15, 16, 18, 19, 20, 37, 38, 39, 46, 47, 48, 50, 51, 52, 54
- binding (2Z)-3-(1H-imidazol-5-yl)-2-iminopropanoic acid: 54, 56, 59, 93
3nycA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase (see paper)
26% identity, 55% coverage: 169:396/413 of query aligns to 122:355/381 of 3nycA
- binding flavin-adenine dinucleotide: E176 (≠ S222), A177 (≠ V223), A205 (≠ L251), G206 (= G252), W208 (= W254), I212 (≠ L258), G309 (= G350), R311 (= R352), Q336 (≠ H377), G337 (= G378), G338 (≠ H379), Y339 (≠ Q380), G340 (= G381), I341 (≠ F382), Q342 (≠ T383)
- binding (2E)-5-[(diaminomethylidene)amino]-2-iminopentanoic acid: R228 (≠ G273), M246 (= M291), E252 (≠ K295), Y255 (≠ L298), R311 (= R352), G338 (≠ H379)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 15, 16, 18, 19, 20, 37, 38, 39, 46, 47, 48, 50, 51, 53, 54
- binding (2E)-5-[(diaminomethylidene)amino]-2-iminopentanoic acid: 59, 93
6j39A Crystal structure of cmis2 with inhibitor (see paper)
25% identity, 97% coverage: 13:413/413 of query aligns to 11:368/368 of 6j39A
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: M44 (≠ I47), P46 (= P55), N49 (≠ T81), R243 (≠ W289), Y252 (≠ L298), Y267 (≠ L314), R308 (= R352), R334 (≠ H379), I335 (≠ Q380)
- binding flavin-adenine dinucleotide: I11 (≠ V13), V29 (= V31), D30 (= D32), P31 (≠ R33), E32 (= E35), K36 (≠ E39), A37 (≠ T40), S38 (= S41), V40 (≠ G43), S41 (≠ N44), A42 (= A45), G43 (= G46), M44 (≠ I47), A174 (≠ V223), A203 (≠ L251), W206 (= W254), I228 (≠ H275), Y252 (≠ L298), R308 (= R352), S333 (≠ G378), R334 (≠ H379), I335 (≠ Q380), G336 (= G381), V337 (≠ F382), Q338 (≠ T383)
Sites not aligning to the query:
6j38A Crystal structure of cmis2 (see paper)
25% identity, 97% coverage: 13:413/413 of query aligns to 11:368/368 of 6j38A
- binding flavin-adenine dinucleotide: I11 (≠ V13), V29 (= V31), D30 (= D32), P31 (≠ R33), K36 (≠ E39), A37 (≠ T40), S38 (= S41), S41 (≠ N44), A42 (= A45), G43 (= G46), M44 (≠ I47), A174 (≠ V223), A203 (≠ L251), W206 (= W254), G226 (= G273), G306 (= G350), R308 (= R352), S333 (≠ G378), R334 (≠ H379), I335 (≠ Q380), G336 (= G381), V337 (≠ F382), Q338 (≠ T383)
Sites not aligning to the query:
Query Sequence
>WP_086511190.1 NCBI__GCF_002151265.1:WP_086511190.1
MARKIIVLGAGMVGVSVAWHLVRRGHDVTLVDRREPGLETSFGNAGIIQREAVRPYAFPR
DVATLLRVVPNRQVDIRYRPTGMLSAAGPLMNYWLNSSGKRYERIVTEWASLIMRCQDAH
APMIEAAGAEALVRKGGWLELYRTQKEFEERQKEAQENHERFGVEYEALDAEALYAKEPH
LAKGLVGAIHWTQPWMVSDPGGLVQAYARSFAAQGGQVKQASVEDVLQVEGGWRVNTSEG
PMEAEQVVVALGPWAGELLGRLGIKVPLFVKRGYHMHYSAEGDAKLNHWVMDAEKGFLLE
PMRAGIRLTTGAELADLDSPPQYKQLAAAEKVARKLFPLGKRRDSQPWKGARPCLPDMKP
VIGPAPNKEGLWLAFGHGHQGFTLGPATGELLAQMMDGEEPAVDMAPFRVDRF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory