SitesBLAST

R175 (= R178) mutation to E: Does not exhibit NADH kinase activity in addition to NAD kinase activity.; mutation to G: Exhibits NADH kinase activity in addition to NAD kinase activity. Reduces the Vmax of the NAD kinase activity.; mutation to H: Exhibits NADH kinase activity in addition to NAD kinase activity.; mutation to I: Does not exhibit NADH kinase activity in addition to NAD kinase activity.; mutation to K: Does not exhibit NADH kinase activity in addition to NAD kinase activity.; mutation to Q: Exhibits NADH kinase activity in addition to NAD kinase activity.; mutation to T: Exhibits NADH kinase activity in addition to NAD kinase activity.

P9WHV7 NAD kinase; ATP-dependent NAD kinase; Poly(P)-dependent NAD kinase; PPNK; EC 2.7.1.23 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
34% identity, 77% coverage: 67:294/297 of query aligns to 76:307/307 of P9WHV7

query
sites
P9WHV7

C

C

D

E

L

L

V

V

I

L

V

V

V

L

G

G

D
|
D

G

G

S

T

L

F

L

L

G

R

A

A

R

E

T

L

L

A

C

R

H

N

S

A

G

S

T

I

L

P

M

V

L

L

G

G

V

V

N

N

R

L

G

G

R

R

L

I

G

G

F

F

L

L

T

A

D

E

I

A

S

E

P

A

D

E

E

A

L

I

E

D

E

A

R

V

V

L

G

E

Q

H

V

V

L

V

D

A

G

Q

K

D

Y

Y

E

R

V

V

E

E

E

D

R

R

F

L

L

T

L

L

D

D

A

V

E

V

L

V

Y

R

R

Q

D

G

G

G

T

R

L

I

V

V

G

N

S

R

G

G

D

W

A

A

L

L

N
|
N

E
|
E

V

V

V

S

L

L

H

E

P

K

G

G

K

P

A

R

V

L

R

G

M

V

I

L

E

G

F

V

E

V

L

V

F

E

I

I

D

D

K

G

Q

R

F

P

V

V

C

S

S

A

Q

F

R

G

S

C

D

D

G
|
G

L

V

I
x
L

M

V

A

S

T
|
T

P
|
P

T
|
T

G
|
G

S
|
S

T
|
T

A
|
A

Y
|
Y

A
|
A

L
x
F

S
|
S

G

A

G
|
G

S

P

I

V

M

L

H

W

P

P

K

D

L

L

D

E

V

A

I

I

E

L

L

V

V

V

P

P

M

N

F

N

P

A

H

H

T

A

L

L

S

F

S

G

R

R

P

P

I

M

V

V

I

T

D

S

A

P

A

E

S

A

E

T

I

I

H

A

V

I

H

E

I

I

G

-

E

E

T

A

N

D

Q

G

T

H

Y

D

P

A

H

L

I

V

S

F

C

C

D

D

G

G

Q

R

T

R

R

E

A

M

V

L

A

I

K

P

P

A

D

G

D

S

V

R

L

L

V

E

V

V

K

T

R

R

K

C

P

V

Q

T

R

S

V

V

Q

K

L

W

V

A

H

R

P

L

L

D

G

S

H

A

N

P

F

F

Y

T

E

D

V

R

L

L

R

V

S

R

K

K

L

F

-

R

-

L

-

P

-

V

-

T

G

G

W

W

S

R

N

G

R

K

D85 (= D76) mutation to A: Abolishes catalytic activity.
N159 (= N150) mutation to A: Abolishes catalytic activity.
NE 159:160 (= NE 150:151) binding
E160 (= E151) mutation to A: Abolishes catalytic activity.
G190 (= G181) mutation to A: Abolishes catalytic activity.
L192 (≠ I183) mutation to A: Abolishes catalytic activity.
T195 (= T186) mutation to A: It promotes stronger allosteric interactions.
P196 (= P187) mutation to A: Abolishes catalytic activity.
T197 (= T188) binding ; mutation to A: Abolishes catalytic activity.
G198 (= G189) mutation to A: Abolishes catalytic activity.
S199 (= S190) mutation to A: Lower catalytic efficiency. A perturbation of the allosteric interactions is observed when NAD is used as substrate.
T200 (= T191) mutation to A: Abolishes catalytic activity.
TAYAFS 200:205 (≠ TAYALS 191:196) binding
Y202 (= Y193) mutation to A: Abolishes catalytic activity.
G207 (= G198) mutation to A: Abolishes catalytic activity.
G208 (= G199) mutation to A: Possesses 30% of the activity compared to the wild-type enzyme. While mutant affects the catalytic efficiency, it does not alter the binding affinity for ATP and poly(P). It causes a decrease in the affinity for NAD and alters the allosteric interactions mediated by the dinucleotide, both in the presence of poly(P) and ATP.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1y3iA Crystal structure of mycobacterium tuberculosis NAD kinase-NAD complex (see paper)
37% identity, 60% coverage: 74:251/297 of query aligns to 12:188/231 of 1y3iA

query
sites
1y3iA

G

G

D
|
D

G
|
G

S

T

L

F

L

L

G

R

A

A

R

E

T

L

L

A

C

R

H

N

S

A

G

S

T

I

L

P

M

V

L

L

G

G

V

V

N

N

R

L

G

G

R
|
R

L

I

G

G

F
|
F

L
|
L

T

A

D

E

I

A

S

E

P

A

D

E

E

A

L

I

E

D

E

A

R

V

V

L

G

E

Q

H

V

V

L

V

D

A

G

Q

K

D

Y

Y

E

R

V

V

E

E

E

D

R

R

F

L

L

T

L

L

D

D

A

V

E

V

L

V

Y

R

R

Q

D

G

G

G

T

R

L

I

V

V

G

N

S

R

G

G

D

W

A

A

L

L

N
|
N

E
|
E

V

V

V

S

L

L

H

E

P

K

G

G

K

P

A

R

V

L

R

G

M

V

I

L

E

G

F

V

E

V

L

V

F

E

I

I

D

D

K

G

Q

R

F

P

V

V

C

S

S

A

Q

F

R

G

S

C

D

D

G

G

L

V

I

L

M

V

A

S

T

T

P

P

T

T

G

G

S

S

T
|
T

A
|
A

Y
|
Y

A

A

L

F

S
|
S

G

A

G

G

S

P

I

V

M

L

H

W

P

P

K

D

L

L

D

E

V

A

I

I

E

L

L

V

V

V

P

P

M

N

F

N

P

A

H

H

T

A

L

L

S

F

S

G

R

R

P

P

I

M

V

V

I

T

D

S

A

P

A

E

S

A

E

T

I

I

H

A

V

I

H

E

I

I

G

-

E

E

T

A

N

D

Q

G

T

H

Y

D

P

A

H

L

I

V

S

F

C

C

D

D

G

G

Q

R

binding nicotinamide-adenine-dinucleotide: D14 (= D76), G15 (= G77), R38 (= R100), F41 (= F103), L42 (= L104), N88 (= N150), E89 (= E151), T129 (= T191), A130 (= A192), Y131 (= Y193), S134 (= S196)

3afoA Crystal structure of yeast nadh kinase complexed with nadh
32% identity, 62% coverage: 68:250/297 of query aligns to 112:301/360 of 3afoA

query
sites
3afoA

D

D

L

L

V

L

I

V

V

T

V

L

G

G

D
|
D

G
|
G

S

T

L

I

L
|
L

G

H

A

G

A

V

R

S

T

M

L

F

C

G

H

N

S

T

G

Q

T

V

L

P

-

P

M

V

L

L

G

A

V

F

N

A

R

L

G

G

R

T

L

L

G

G

F
|
F

L

L

T

S

D

P

I

F

S

D

P

F

D

K

E

E

L

H

E

K

R

V

V

F

G

Q

Q

E

V

V

L

I

D

S

G

S

K

R

Y

A

E

K

V

C

E

L

E

H

R

R

F

T

L

R

L

L

D

E

A

C

E

H

L

L

Y

K

R

K

-

K

D

D

G

S

T

N

L

S

V

S

G

I

S

V

G

T

D

H

A

A

L

M

N
|
N

E
x
D

V

I

V

F

L

L

H

H

P

R

G

G

K

N

A

S

V

P

R

H

M

L

I

T

E

N

F

L

E

D

L

I

F

F

I

I

D

D

K

G

Q

E

F

F

V

L

C

T

S

R

Q

T

R

T

S

A

D

D

G

G

L

V

I

A

M

L

A

A

T

T

P

P

T

T

G

G

S

S

T
|
T

A
|
A

Y
|
Y

A

S

L

L

S
|
S

G

A

G

G

S

S

I

I

M

V

H

S

P

P

K

L

L

V

D

P

V

A

I

I

E

L

L

M

V

T

P

P

M

I

F

C

P

P

H

R

T

S

L

L

S

S

S

F

R

R

P

P

I

L

V

I

I

L

D

P

A

H

A

S

S

S

E

H

I

I

H

R

V

I

H

K

I

I

G

G

E

S

T

K

N

L

Q

N

T

Q

Y

K

P

P

-

V

-

N

-

S

-

V

H

K

I

L

S

S

C

V

D
|
D

G
|
G

binding 1,4-dihydronicotinamide adenine dinucleotide: D120 (= D76), G121 (= G77), L124 (= L80), F148 (= F103), N196 (= N150), D197 (≠ E151), T237 (= T191), A238 (= A192), Y239 (= Y193), S242 (= S196), D300 (= D249), G301 (= G250)

O13863 Uncharacterized kinase C1B1.02c; EC 2.7.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 80% coverage: 60:296/297 of query aligns to 265:516/537 of O13863

query
sites
O13863

R

R

R

F

T

W

L

T

G

S

E

E

L

L

C

C

-

T

-

Q

-

C

-

P

-

N

-

L

-

F

D

D

L

C

V

V

I

I

V

T

V

V

G

G

G

D

D

D

G

S

S

A

L

A

L

L

G

R

A

A

A

S

R

W

T

L

L

F

C

Q

H

D

S

V

G

V

T

P

L

P

M

V

L

L

G

S

V

F

N

S

R

T

G

A

R

K

L

A

G

G

F

F

L

L

T

S

D

I

I

L

S

P

P

I

D

A

E

E

L

Y

E

T

E

K

R

T

V

L

G

D

Q

L

V

I

L

F

D

H

G

R

K

G

Y

F

E

T

V

V

E

N

E

L

R

R

F

M

L

R

L

F

D

Q

A

C

E

S

L

I

Y

M

R

R

-

Y

-

V

-

G

-

E

-

H

-

S

D

T

G

H

T

I

L

C

V

E

G

G

S

Q

G

Y

D

S

A

V

L

L

N

N

E

E

V

L

L

I

H

D

P

R

G

G

K

P

A

N

V

P

R

F

M

M

I

I

E

S

F

L

E

D

L

L

F

Y

I

V

D

E

K

N

Q

E

F

Y

V

I

C

T

S

T

Q

L

R

Q

S

S

D

D

G

G

L

V

I

C

M

V

A

S

T

T

P

P

T

T

G

G

S

S

T

T

A

A

Y

Y

A

S

L

V

S

A

G

A

G

G

S

S

I

L

M

C

H

H

P

P

K

G

L

I

D

P

V

A

I

I

E

L

L

I

V

S

P

A

M

I

F

C

P

P

H

H

T

S

L

L

S

S

S

F

R

R

P

P

I

I

V

I

I

L

D

P

A

D

A

S

S

M

E

T

I

L

H

R

V

I

H

V

I

V

G

P

E

L

T

D

N

A

Q

R

T

S

Y

N

P

A

H

W

I

C

S

A

C

F

D

D

G

G

Q

H

T

H

R

R

A

I

V

E

A

L

K

G

P

L

D

G

D

D

V

Y

L

I

V

S

V

I

K

S

R

A

K

S

-

S

-

F

P

P

Q

F

R

P

V

S

Q

V

L

I

V

R

H

S

P

K

L

Y

G

S

H

K

N

D

F

W

Y

F

E

D

V

I

L

L

R

R

S

Q

K

T

L

L

G

N

W

W

S

N

N

D

R

R

L

K

G

G

Sites not aligning to the query:

72 modified: Phosphoserine

1z0zA Crystal structure of a NAD kinase from archaeoglobus fulgidus in complex with NAD (see paper)
34% identity, 56% coverage: 68:234/297 of query aligns to 41:199/249 of 1z0zA

query
sites
1z0zA

D

D

L

F

V

I

I

V

V

S

V

V

G

G

D

D

G

G

S

T

L

I

L

L

G

R

A

I

A

L

R

Q

T

K

L

L

C

K

H

R

S

C

G

P

T

P

L

I

M

-

L

F

G

G

V

I

N

N

R

T

G

G

R

R

L

V

G

G

F

L

L

L

T

T

D

H

I

A

S

S

P

P

D

E

E

N

L

F

E

E

E

V

R

E

V

L

G

K

Q

K

V

A

L

V

D

E

G

-

K

K

Y

F

E
|
E

V

V

E

E

E

R

R

F

F

P

L

R

L

V

D

S

A
x
C

E

S

L

A

Y

M

R

P

D

D

G

V

T

L

L

-

V

-

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

I

V

A

L

V

H

L

P

S

G

R

K

K

A

P

V

A

R

K

M
|
M

I

I

E

D

F

V

E

A

L

L

F

R

I

V

D

D

K

G

Q

V

F

E

V

V

C

D

S

R

Q

I

R
|
R

S

C

D
|
D

G

G

L

F

I

I

M

V

A

A

T

T

P

Q

T

I

G

G

S

S

T
|
T

A

G

Y
|
Y

A

A

L

F

S
|
S

G

A

G

G

S

P

I

V

M

V

H

E

P

P

K

Y

L

L

D

E

V

C

I

F

E

I

L

L

V

I

P

P

M

I

F

A

P

P

H
x
F

T

R

L

F

S

G

S

W

R

K

P

P

I

Y

V

V

I

V

D

S

A

M

A

E

S

R

E

K

I

I

H

E

V

V

active site: E96 (= E125), C105 (≠ A134)
binding nicotinamide-adenine-dinucleotide: N115 (= N150), E116 (= E151), M127 (= M162), R143 (= R178), D145 (= D180), T156 (= T191), Y158 (= Y193), S161 (= S196), F182 (≠ H217)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 209, 210

1z0sA Crystal structure of an NAD kinase from archaeoglobus fulgidus in complex with atp (see paper)
34% identity, 56% coverage: 68:234/297 of query aligns to 41:199/249 of 1z0sA

query
sites
1z0sA

D

D

L

F

V

I

I

V

V

S

V

V

G

G

G
|
G

D

D

G
|
G

S
x
T

L

I

L

L

G
x
R

A

I

A

L

R

Q

T

K

L

L

C

K

H

R

S

C

G

P

T

P

L

I

M

-

L

F

G

G

V

I

N

N

R

T

G

G

R
|
R

L

V

G

G

F

L

L

L

T

T

D

H

I

A

S

S

P

P

D

E

E

N

L

F

E

E

E

V

R

E

V

L

G

K

Q

K

V

A

L

V

D

E

G

-

K

K

Y

F

E
|
E

V

V

E

E

E

R

R

F

F

P

L

R

L

V

D

S

A
x
C

E

S

L

A

Y

M

R

P

D

D

G

V

T

L

L

-

V

-

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

I

V

A

L

V

H

L

P

S

G

R

K

K

A

P

V
x
A

R
x
K

M
|
M

I

I

E

D

F

V

E

A

L

L

F

R

I

V

D

D

K

G

Q

V

F

E

V

V

C

D

S

R

Q

I

R

R

S

C

D
|
D

G

G

L

F

I

I

M

V

A

A

T

T

P

Q

T

I

G

G

S

S

T

T

A
x
G

Y
|
Y

A

A

L

F

S
|
S

G

A

G

G

S

P

I

V

M

V

H

E

P

P

K

Y

L

L

D

E

V

C

I

F

E

I

L

L

V

I

P

P

M

I

F
x
A

P

P

H
x
F

T

R

L

F

S

G

S

W

R

K

P

P

I

Y

V

V

I

V

D

S

A

M

A

E

S

R

E

K

I

I

H

E

V

V

active site: E96 (= E125), C105 (≠ A134)
binding adenosine-5'-triphosphate: R54 (≠ G81), N115 (= N150), E116 (= E151), A125 (≠ V160), K126 (≠ R161), M127 (= M162), D145 (= D180), G157 (≠ A192), Y158 (= Y193), S161 (= S196), A180 (≠ F215), F182 (≠ H217)
binding pyrophosphate 2-: G48 (= G75), G50 (= G77), T51 (≠ S78), R54 (≠ G81), R72 (= R100)

Sites not aligning to the query:

1suwA Crystal structure of a NAD kinase from archaeoglobus fulgidus in complex with its substrate and product: insights into the catalysis of NAD kinase (see paper)
34% identity, 56% coverage: 68:234/297 of query aligns to 41:199/249 of 1suwA

query
sites
1suwA

D

D

L

F

V

I

I

V

V

S

V

V

G

G

G
|
G

D
|
D

G
|
G

S

T

L

I

L

L

G

R

A

I

A

L

R

Q

T

K

L

L

C

K

H

R

S

C

G

P

T

P

L

I

M

-

L

F

G

G

V

I

N

N

R

T

G

G

R

R

L

V

G

G

F

L

L

L

T

T

D

H

I

A

S

S

P

P

D

E

E

N

L

F

E

E

E

V

R

E

V

L

G

K

Q

K

V

A

L

V

D

E

G

-

K

K

Y

F

E

E

V

V

E

E

E

R

R

F

F

P

L

R

L

V

D

S

A

C

E

S

L

A

Y

M

R

P

D

D

G

V

T

L

L

-

V

-

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

I

V

A

L

V

H

L

P

S

G

R

K

K

A

P

V
x
A

R

K

M
|
M

I

I

E

D

F

V

E

A

L

L

F

R

I

V

D

D

K

G

Q

V

F

E

V

V

C

D

S

R

Q

I

R
|
R

S

C

D
|
D

G

G

L

F

I

I

M

V

A

A

T

T

P

Q

T

I

G

G

S

S

T
|
T

A

G

Y
|
Y

A

A

L

F

S
|
S

G

A

G

G

S

P

I

V

M

V

H

E

P

P

K

Y

L

L

D

E

V

C

I

F

E

I

L

L

V

I

P

P

M

I

F

A

P

P

H
x
F

T

R

L

F

S

G

S

W

R

K

P

P

I

Y

V

V

I

V

D

S

A

M

A

E

S

R

E

K

I

I

H

E

V

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G48 (= G75), D49 (= D76), G50 (= G77), N115 (= N150), E116 (= E151), A125 (≠ V160), M127 (= M162), R143 (= R178), D145 (= D180), T156 (= T191), Y158 (= Y193), S161 (= S196), F182 (≠ H217)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 209, 210

O30297 NAD kinase; ATP-dependent NAD kinase; EC 2.7.1.23 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
34% identity, 56% coverage: 68:234/297 of query aligns to 41:199/249 of O30297

query
sites
O30297

D

D

L

F

V

I

I

V

V

S

V

V

G

G

D
|
D

G
|
G

S

T

L

I

L

L

G
x
R

A

I

A

L

R

Q

T

K

L

L

C

K

H

R

S

C

G

P

T

P

L

I

M

-

L

F

G

G

V

I

N

N

R

T

G

G

R

R

L

V

G

G

F

L

L

L

T

T

D

H

I

A

S

S

P

P

D

E

E

N

L

F

E

E

E

V

R

E

V

L

G

K

Q

K

V

A

L

V

D

E

G

-

K

K

Y

F

E

E

V

V

E

E

E

R

R

F

F

P

L

R

L

V

D

S

A

C

E

S

L

A

Y

M

R

P

D

D

G

V

T

L

L

-

V

-

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

I

V

A

L

V

H

L

P

S

G

R

K

K

A

P

V

A

R
x
K

M

M

I

I

E

D

F

V

E

A

L

L

F

R

I

V

D

D

K

G

Q

V

F

E

V

V

C

D

S

R

Q

I

R
|
R

S

C

D

D

G

G

L

F

I

I

M

V

A

A

T

T

P

Q

T
x
I

G

G

S

S

T
|
T

A
x
G

Y
|
Y

A
|
A

L
x
F

S
|
S

G

A

G

G

S

P

I

V

M

V

H

E

P

P

K

Y

L

L

D

E

V

C

I

F

E

I

L

L

V

I

P

P

M

I

F

A

P

P

H

F

T

R

L

F

S

G

S

W

R

K

P

P

I

Y

V

V

I

V

D

S

A

M

A

E

S

R

E

K

I

I

H

E

V

V

DG 49:50 (= DG 76:77) binding
R54 (≠ G81) binding
NE 115:116 (= NE 150:151) binding
K126 (≠ R161) binding
R143 (= R178) binding
I153 (≠ T188) binding
TGYAFS 156:161 (≠ TAYALS 191:196) binding

Sites not aligning to the query:

211 binding

Q9P7K3 Uncharacterized kinase C24B10.02c; EC 2.7.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
26% identity, 77% coverage: 66:294/297 of query aligns to 174:409/449 of Q9P7K3

query
sites
Q9P7K3

L

I

C

F

D

D

L

L

V

A

I

I

V

T

V

I

G

G

G

D

D

N

G

S

S

T

L

L

G

Y

A

T

A

S

R

W

T

L

L

F

C

Q

H

K

S

I

G

G

T

P

L

P

M

V

L

L

G

S

V

F

N

S

R

D

G

D

R

D

L

V

-

P

G

G

F

F

L

L

T

T

D

H

I

F

S

S

P

L

D

S

E

N

L

Y

E

Q

R

H

V

L

G

Y

Q

Q

V

V

L

L

D

T

G

Q

K

N

Y

V

E

S

V

L

E

R

-

F

-

C

E

S

R

R

F

L

L

Q

L

C

D

S

A

F

E

H

L

K

Y

Y

R

D

D

E

G

K

T

T

-

K

-

Q

-

Y

-

S

L

L

V

A

G

S

S

T

G

T

D

Y

A

S

L

L

N

D

E

E

V

I

V

L

L

I

H

S

P

R

G

G

K

E

A

H

V

P

R

F

M

I

I

S

E

N

F

L

E

N

L

V

F

Y

I

N

D

N

K

S

Q

E

F

L

V

M

C

T

S

V

Q

V

R

Q

S

A

D

D

G

G

L

L

I

V

M

V

A

A

T

T

P

P

T

T

G

G

S

S

T

T

A

N

Y

I

A

S

L

A

S

N

G

A

G

G

S

S

I

L

M

V

H

H

P

P

K

A

L

L

D

N

V

A

I

I

E

L

L

V

V

T

P

P

M

V

F

C

P

P

H

H

T

T

L

L

S

S

S

F

R

R

P

P

I

I

V

I

I

L

D

P

A

D

A

Y

S

N

E

V

I

L

H

N

V

V

H

E

I

I

G

P

E

L

T

D

N

S

Q

R

T

S

Y

S

P

A

H

F

I

F

S

S

C

V

D

D

G

R

Q

H

T

E

R

S

A

V

V

E

A

M

K

H

P

R

D

G

D

D

V

Y

L

L

V

S

V

I

K

V

R

T

K

S

P

H

Q

Y

R

P

V

F

Q

T

L

T

V

I

H

Q

P

N

L

P

G

G

H

Y

N

Q

F

W

Y

T

E

K

V

V

L

L

R

E

S

D

K

K

L

F

G

N

W

W

S

N

N

V

R

R

Sites not aligning to the query:

420 modified: Phosphoserine

2i2bA Crystal structure of lmnadk1 from listeria monocytogenes (see paper)
32% identity, 49% coverage: 68:214/297 of query aligns to 37:180/258 of 2i2bA

query
sites
2i2bA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

D
|
D

G

G

S

T

L

F

L

L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R

H

L

L

G

G

F
|
F

L
x
Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

-

K

Y

Y

R

K

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding beta-d-erythrofuranosyl-adenosine: D45 (= D76), F74 (= F103), Y75 (≠ L104), N118 (= N150), N118 (= N150), E119 (= E151), T157 (= T191), A158 (= A192), Y159 (= Y193), S162 (= S196)

Sites not aligning to the query:

binding glycerol: 249, 250, 253

3v8pA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a new di-adenosine inhibitor formed in situ (see paper)
32% identity, 49% coverage: 68:214/297 of query aligns to 37:180/260 of 3v8pA

query
sites
3v8pA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

D
|
D

G
|
G

S

T

L

F

L
|
L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R

H

L

L

G

G

F
|
F

L
x
Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

-

K

Y

Y

R

K

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding 2-[6-azanyl-9-[(2R,3R,4S,5R)-5-[[(azanylidene-$l^{4}-azanylidene)amino]methyl]-3,4-bis(oxidanyl)oxolan-2-yl]purin-8-yl]sulfanyl-N-[[(2R,3S,4R,5R)-5-(6-azanyl-8-bromanyl-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl]ethanamide: D45 (= D76), G46 (= G77), L49 (= L80), F74 (= F103), Y75 (≠ L104), N118 (= N150), E119 (= E151), T157 (= T191), A158 (= A192), Y159 (= Y193), S162 (= S196)

Sites not aligning to the query:

binding 2-[6-azanyl-9-[(2R,3R,4S,5R)-5-[[(azanylidene-$l^{4}-azanylidene)amino]methyl]-3,4-bis(oxidanyl)oxolan-2-yl]purin-8-yl]sulfanyl-N-[[(2R,3S,4R,5R)-5-(6-azanyl-8-bromanyl-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl]ethanamide: 218

3v7wA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with 5'-azido-5'-deoxyadenosine (see paper)
32% identity, 49% coverage: 68:214/297 of query aligns to 37:180/260 of 3v7wA

query
sites
3v7wA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

D
|
D

G
|
G

S

T

L

F

L

L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R

H

L
|
L

G

G

F
|
F

L
x
Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

-

K

Y

Y

R

K

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding 5'-azido-5'-deoxyadenosine: D45 (= D76), G46 (= G77), L72 (= L101), F74 (= F103), Y75 (≠ L104), N118 (= N150), N118 (= N150), E119 (= E151), T157 (= T191), A158 (= A192), Y159 (= Y193), S162 (= S196)

4dy6A Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with 2'-phosphate bis(adenosine)-5'-diphosphate (see paper)
31% identity, 49% coverage: 68:214/297 of query aligns to 37:179/258 of 4dy6A

query
sites
4dy6A

D

E

L

I

V

V

I

I

V

S

V

I

G

G

D
|
D

G
|
G

S

T

L

F

L

L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R
x
H

L

L

G

G

F
|
F

L

Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

Y

K

R

Y

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding [(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl[(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl dihydrogen diphosphate: D45 (= D76), G46 (= G77), H71 (≠ R100), F74 (= F103), N117 (= N150), E118 (= E151), T156 (= T191), A157 (= A192), Y158 (= Y193), S161 (= S196)

6rgeA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with an inhibitor
31% identity, 49% coverage: 68:214/297 of query aligns to 37:179/259 of 6rgeA

query
sites
6rgeA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

G
|
G

D
|
D

G
|
G

S

T

L

F

L
|
L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R
x
H

L

L

G

G

F
|
F

L

Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

Y

K

R

Y

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding [(2~{R},3~{R},4~{R},5~{R})-2-[8-[3-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy]prop-1-ynyl]-6-azanyl-purin-9-yl]-5-(hydroxymethyl)-4-oxidanyl-oxolan-3-yl] dihydrogen phosphate: G44 (= G75), D45 (= D76), G46 (= G77), L49 (= L80), H71 (≠ R100), F74 (= F103), N117 (= N150), E118 (= E151), T156 (= T191), A157 (= A192), Y158 (= Y193), S161 (= S196)

6rgcA Crystal structure of NAD kinase 1 from listeria monocytogenes in complexe with an inhibitor (see paper)
31% identity, 49% coverage: 68:214/297 of query aligns to 37:179/259 of 6rgcA

query
sites
6rgcA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

D
|
D

G
|
G

S

T

L

F

L

L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R

H

L
|
L

G

G

F
|
F

L

Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

Y

K

R

Y

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding (2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-[[3-[6-azanyl-9-[(2~{R},3~{R},4~{S},5~{R})-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]purin-8-yl]prop-2-ynyl-methyl-amino]methyl]oxolane-3,4-diol: D45 (= D76), G46 (= G77), L72 (= L101), F74 (= F103), N117 (= N150), E118 (= E151), T156 (= T191), A157 (= A192), Y158 (= Y193), S161 (= S196)

6rgaA Crystal structure of NAD kinase 1 from listeria monocytogenes in complexe with an inhibitor (see paper)
31% identity, 49% coverage: 68:214/297 of query aligns to 37:179/259 of 6rgaA

query
sites
6rgaA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

D
|
D

G

G

S

T

L

F

L
|
L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R

H

L

L

G

G

F
|
F

L

Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

Y

K

R

Y

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding (2~{R},3~{S},4~{R},5~{R})-2-(aminomethyl)-5-[8-[3-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy]prop-1-ynyl]-6-azanyl-purin-9-yl]oxolane-3,4-diol: D45 (= D76), L49 (= L80), F74 (= F103), N117 (= N150), E118 (= E151), T156 (= T191), A157 (= A192), Y158 (= Y193), S161 (= S196)

5ejiA Crystal structure of NAD kinase w78f mutant from listeria monocytogenes in complex with NADP/mn++/ppi
32% identity, 49% coverage: 68:214/297 of query aligns to 37:180/260 of 5ejiA

query
sites
5ejiA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

G
|
G

D
|
D

G
|
G

S
x
T

L

F

L

L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R

H

L
|
L

G
|
G

F
|
F

L
x
Y

T

A

D

D

I

F

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

-

K

Y

Y

R

G

D

E

G

A

T

T

L

Y

V

L

G

-

S

-

G

-

D

-

A

A

L

L

N
|
N

E
|
E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y
|
Y

A

N

L

K

S
|
S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding nadp nicotinamide-adenine-dinucleotide phosphate: G44 (= G75), D45 (= D76), L72 (= L101), G73 (= G102), F74 (= F103), Y75 (≠ L104), N118 (= N150), E119 (= E151), T157 (= T191), A158 (= A192), Y159 (= Y193), S162 (= S196)
binding pyrophosphate 2-: G44 (= G75), D45 (= D76), G46 (= G77), T47 (≠ S78)

6rbzA Crystal structure of NAD kinase 1 from listeria monocytogenes in complexe with an adenine derivative (see paper)
31% identity, 49% coverage: 68:214/297 of query aligns to 37:182/262 of 6rbzA

query
sites
6rbzA

D

E

L

I

V

V

I

I

V

S

V

I

G

G

D
|
D

G
|
G

S

T

L

F

L

L

G

S

A

A

A

F

R

H

T

Q

L

Y

C

E

H

E

-

R

-

L

S

D

G

E

T

I

L

A

M

F

L

I

G

G

V

I

N

H

R

T

G

G

R

H

L

L

G

G

F
|
F

L

Y

T

A

D

D

I

W

S

R

P

P

D

A

E

E

L

A

E

D

E

K

R

L

V

V

G

K

Q

L

V

L

L

A

D

K

G

G

K

E

Y

Y

E

Q

V

K

E

V

E

S

R

Y

F

P

L

L

D

K

A

T

E

T

L

V

Y

-

R

K

D

Y

G

G

T

K

L

K

V

E

G

A

S

T

G

Y

D

L

A

A

L

L

N
|
N

E

E

V

S

V

T

L

V

H

K

P

S

G

S

K

G

-

G

-

P

-

F

-

V

-

D

-

V

A

V

V

I

R

N

M

D

I

I

E

H

F

F

E

E

L

R

F

F

I

-

D

-

K

-

Q

-

F

-

V

-

C

-

S

-

Q

-

R

R

S

G

D

D

G

G

L

L

I

C

M

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

A
|
A

Y

Y

A

N

L

K

S

S

G

L

G

G

S

A

I

L

M

M

H

H

P

P

K

S

L

I

D

E

V

A

I

M

E

Q

L

L

V

T

P

E

M

M

binding 9-(3-azanylpropyl)-8-bromanyl-purin-6-amine: D45 (= D76), G46 (= G77), F74 (= F103), N120 (= N150), T159 (= T191), A160 (= A192)

Query Sequence

>WP_086511673.1 NCBI__GCF_002151265.1:WP_086511673.1
MHKQHDGFKNIGLIGRLGSTQVVETLERLLRLLDERGLHVIVEDRTATVLPDHGHPEASR
RTLGELCDLVIVVGGDGSLLGAARTLCHSGTLMLGVNRGRLGFLTDISPDELEERVGQVL
DGKYEVEERFLLDAELYRDGTLVGSGDALNEVVLHPGKAVRMIEFELFIDKQFVCSQRSD
GLIMATPTGSTAYALSGGGSIMHPKLDVIELVPMFPHTLSSRPIVIDAASEIHVHIGETN
QTYPHISCDGQTRAVAKPDDVLVVKRKPQRVQLVHPLGHNFYEVLRSKLGWSNRLGD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory