SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086511696.1 NCBI__GCF_002151265.1:WP_086511696.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8VBT2 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Mus musculus (Mouse)
48% identity, 97% coverage: 2:299/308 of query aligns to 6:314/327 of Q8VBT2

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

1pwhA Rat liver l-serine dehydratase- complex with pyridoxyl-(o-methyl- serine)-5-monophosphate (see paper)
47% identity, 97% coverage: 2:299/308 of query aligns to 6:314/327 of 1pwhA

query
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active site: K41 (= K37), A65 (≠ G61), E194 (= E187), A198 (= A191), S200 (= S193), A222 (≠ S215), A269 (= A262), C303 (= C288)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-o-methyl-l-serine: F40 (= F36), K41 (= K37), S64 (= S60), A65 (≠ G61), N67 (= N63), A68 (= A64), F136 (= F130), G168 (= G161), G169 (= G162), G170 (= G163), G171 (= G164), A222 (≠ S215), G224 (= G217), C303 (= C288), G304 (= G289)

P20132 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Homo sapiens (Human) (see paper)
49% identity, 96% coverage: 3:299/308 of query aligns to 7:314/328 of P20132

query
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P20132

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C303 (= C288) mutation to A: Loss of enzyme activity.

P09367 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Rattus norvegicus (Rat) (see paper)
42% identity, 97% coverage: 2:299/308 of query aligns to 6:350/363 of P09367

query
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P09367

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

1p5jA Crystal structure analysis of human serine dehydratase (see paper)
48% identity, 96% coverage: 3:299/308 of query aligns to 4:311/319 of 1p5jA

query
sites
1p5jA

L

L

H

H

I

V

E

K

T

T

P

P

L

I

I

R

E

D

S

S

R

M

A

A

M

L

S

S

V

K

M

M

T

A

G

G

R

T

S

S

V

V

W

Y

I

L

K

K

L

M

D

D

A

S

L

A

Q

Q

P

P

P

S

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

I

G

G

H

H

A

F

C

C

E

K

T

R

Y

W

L

A

A

K

R

Q

G

G

A

C

R

A

R

H

F

F

V

V

A

C

S

S

G
x
A

G

G

N
|
N

A

A

G

G

L

M

A

A

V

A

A

A

Y

Y

A

A

G

A

R

R

R

Q

L

L

G

G

V

V

P

P

V

A

T

T

V

I

V

V

P

P

E

G

T

T

T

P

E

A

R

L

A

T

K

I

K

E

L

R

L

L

R

K

L

N

E

E

T

G

A

A

E

T

V

C

I

K

V

V

H

V

G

G

A

E

S

L

W

L

Q

D

E

E

A

A

N

F

A

E

F

L

A

A

Q

K

S

A

L

L

I

A

G

K

A

N

S

N

D

P

A

G

F

W

L

V

H

Y

-

I

-

P

P

P

F

F

D

D

P

P

L

L

L

I

W

W

Q

E

G

G

H

H

A

A

T

S

L

I

I

V

D

K

E

E

V

L

F

K

C

E

S

T

G

L

L

W

-

E

K

K

P

P

D

G

A

A

V

I

V

A

L

L

S

S

V
|
V

G
|
G

L

L

S

C

G

G

V

V

E

Q

G

G

L

L

H

Q

R

E

N

C

G

G

W

W

S

G

D

D

I

V

P

P

V

V

F

I

A

A

V

M

E
|
E

T

T

T

F

G

G

A
|
A

A

H

S
|
S

F

F

H

H

A

A

V

T

R

T

A

A

G

G

H

K

T

L

V

V

E

S

L

L

E

P

K

K

I

I

I

T

S

S

V

V

A

A

T

K

S
x
A

L

L

G

G

A

V

K

K

R

T

V

V

C

G

E

S

K

Q

A

A

F

L

Q

K

W

L

S

F

K

Q

N

E

H

H

P

P

I

I

H

F

S

S

V

E

V

V

V

I

S

S

D

D

R

Q

S

E

A

A

L

V

S

A

A

A

C

I

E

E

R

K

F

F

L

V

A

D

D

D

H

E

R

K

I

I

L

L

V

V

E

E

P

P

A

A

C

C

G

G

A

A

S

A

L

L

A

A

I

A

A

V

Y

Y

E

S

N

H

A

V

S

I

-

Q

-

K

-

L

E

Q

L

L

E

E

N

G

-

N

-

L

-

R

-

T

-

P

F

L

S

P

N

S

I

L

L

V

V

V

V

I

V

V

C
|
C

G
|
G

A

S

T

N

T

I

T

S

I

L

D

A

Q

Q

I

L

R

R

active site: K38 (= K37), A62 (≠ G61), E191 (= E187), A195 (= A191), S197 (= S193), A219 (≠ S215), C300 (= C288)
binding pyridoxal-5'-phosphate: F37 (= F36), K38 (= K37), N64 (= N63), V164 (= V160), G165 (= G161), G166 (= G162), G167 (= G163), G168 (= G164), A219 (≠ S215), C300 (= C288), G301 (= G289), G302 (= G290)

Q96GA7 Serine dehydratase-like; L-serine deaminase; L-serine dehydratase/L-threonine deaminase; L-threonine dehydratase; TDH; Serine dehydratase 2; SDH 2; EC 4.3.1.19; EC 4.3.1.17 from Homo sapiens (Human) (see paper)
47% identity, 94% coverage: 3:290/308 of query aligns to 14:311/329 of Q96GA7

query
sites
Q96GA7

L

F

H

H

I

V

E

V

T

T

P

P

L

L

I

L

E

E

S

S

R

W

A

A

M

L

S

S

V

Q

M

V

T

A

G

G

R

M

S

P

V

V

W

F

I

L

K

K

L

C

D

E

A

N

L

V

Q

Q

P

P

P

S

G

G

S

S

F

F

K

K

I

I

R

R

G

G

I

I

G

G

H

H

A

F

C

C

E

Q

T

E

Y

M

L

A

A

K

R

K

G

G

A

C

R

R

R

H

F

L

V

V

A

C

S

S

G
|
G

G

G

N

N

A

A

G

G

L

I

A

A

V

A

A

A

Y

Y

A

A

G

A

R

R

R

K

L

L

G

G

V

I

P

P

V

A

T

T

V

I

V

V

V

L

P

P

E

E

T

S

T

T

T

S

E

L

R

Q

A

V

K

V

K

Q

L

R

L

L

R

Q

L

G

E

E

T

G

A

A

E

E

V

V

I

Q

V

L

H

T

G

G

A

K

S

V

W

W

Q

D

E

E

A

A

N

N

A

L

F

R

A

A

Q

Q

S

E

L

L

I

-

G

A

A

K

S

R

D

D

A

G

F

W

-

E

-

N

L

V

H

P

P

P

F

F

D

D

D

H

P

P

L

L

L

I

W

W

Q

K

G

G

H

H

A

A

T

S

L

L

I

V

D

Q

E

E

V

L

F

K

C

A

S

V

-

L

G

R

L

T

K

P

P

P

D

G

A

A

V

L

V

V

L

L

S

A

V

V

G

G

L

L

S

A

G

G

V

V

E

A

G

G

L

L

H

L

R

E

N

V

G

G

W

W

S

Q

D

H

I

V

P

P

V

I

F

I

A

A

V

M

E

E

T

T

T

H

G

G

A

A

A

H

S

C

F

F

H

N

A

A

V

I

R

T

A

A

G

G

H

K

T

L

V

V

E

T

L

L

E

P

K

D

I

I

I

T

S

S

V

V

A

A

T

K

S

S

L

L

G

G

A

A

K

K

R

T

V

V

C

A

E

A

K

R

A

A

F

L

Q

E

W

C

S

M

K

Q

N

V

H

C

P

K

I

I

H

H

S

S

V

E

V

V

S

E

D

D

R

T

S

E

A

A

L

V

S

S

A

A

C

V

E

Q

R

Q

F

L

L

L

A

D

D

D

H

E

R

R

I

M

L

L

V

V

E

E

P

P

A

A

C

C

G

G

A

A

S

A

L

L

A

A

I

A

A

I

Y

Y

E

S

-

G

-

L

-

R

-

L

-

Q

-

A

N

E

A

G

S

C

E

L

L

P

E

P

N

S

F

L

S

T

N

S

I

V

L

V

V

V

V

I

V

V

C
|
C

G

G

G72 (= G61) mutation to A: Strongly increased enzyme activity towards threonine.
C309 (= C288) mutation to A: Loss of enzyme activity.

2rkbA Serine dehydratase like-1 from human cancer cells (see paper)
47% identity, 94% coverage: 3:290/308 of query aligns to 4:301/318 of 2rkbA

query
sites
2rkbA

L

F

H

H

I

V

E

V

T

T

P

P

L

L

I

L

E

E

S

S

R

W

A

A

M

L

S

S

V

Q

M

V

T

A

G

G

R

M

S

P

V

V

W

F

I

L

K

K

L

C

D

E

A

N

L

V

Q

Q

P

P

P

S

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

I

G

G

H

H

A

F

C

C

E

Q

T

E

Y

M

L

A

A

K

R

K

G

G

A

C

R

R

R

H

F

L

V

V

A

C

S

S

G
|
G

G

G

N
|
N

A

A

G

G

L

I

A

A

V

A

A

A

Y

Y

A

A

G

A

R

R

R

K

L

L

G

G

V

I

P

P

V

A

T

T

V

I

V

V

V

L

P

P

E

E

T

S

T

T

T

S

E

L

R

Q

A

V

K

V

K

Q

L

R

L

L

R

Q

L

G

E

E

T

G

A

A

E

E

V

V

I

Q

V

L

H

T

G

G

A

K

S

V

W

W

Q

D

E

E

A

A

N

N

A

L

F

R

A

A

Q

Q

S

E

L

L

I

-

G

A

A

K

S

R

D

D

A

G

F

W

-

E

-

N

L

V

H

P

P

P

F

F

D

D

D

H

P

P

L

L

L

I

W

W

Q

K

G

G

H

H

A

A

T

S

L

L

I

V

D

Q

E

E

V

L

F

K

C

A

S

V

-

L

G

R

L

T

K

P

P

P

D

G

A

A

V

L

V

V

L

L

S

A

V

V

G
|
G

L

L

S

A

G

G

V

V

E

A

G

G

L

L

H

L

R

E

N

V

G

G

W

W

S

Q

D

H

I

V

P

P

V

I

F

I

A

A

V

M

E
|
E

T

T

T

H

G

G

A
|
A

A

H

S
x
C

F

F

H

N

A

A

V

I

R

T

A

A

G

G

H

K

T

L

V

V

E

T

L

L

E

P

K

D

I

I

I

T

S

S

V

V

A

A

T

K

S
|
S

L

L

G

G

A

A

K

K

R

T

V

V

C

A

E

A

K

R

A

A

F

L

Q

E

W

C

S

M

K

Q

N

V

H

C

P

K

I

I

H

H

S

S

V

E

V

V

S

E

D

D

R

T

S

E

A

A

L

V

S

S

A

A

C

V

E

Q

R

Q

F

L

L

L

A

D

D

D

H

E

R

R

I

M

L

L

V

V

E

E

P

P

A
|
A

C

C

G

G

A

A

S

A

L

L

A

A

I

A

A

I

Y

Y

E

S

-

G

-

L

-

R

-

L

-

Q

-

A

N

E

A

G

S

C

E

L

L

P

E

P

N

S

F

L

S

T

N

S

I

V

L

V

V

V

V

I

V

V

C
|
C

G

G

active site: K38 (= K37), G62 (= G61), E190 (= E187), A194 (= A191), C196 (≠ S193), S218 (= S215), A265 (= A262), C299 (= C288)
binding pyridoxal-5'-phosphate: F37 (= F36), K38 (= K37), N64 (= N63), G164 (= G161), G165 (= G162), G166 (= G163), G167 (= G164), S218 (= S215), C299 (= C288)

5c3uA Crystal structure of a fungal l-serine ammonia-lyase from rhizomucor miehei (see paper)
37% identity, 97% coverage: 2:301/308 of query aligns to 2:310/315 of 5c3uA

query
sites
5c3uA

T

S

L

L

H

H

I

V

E

A

T

T

P

P

L

L

I

L

E

H

S

A

R

P

A

N

M

L

S

T

V

K

M

E

T

L

G

E

R

C

S

N

V

V

W

F

I

L

K

K

L

M

D

E

A

N

L

I

Q

Q

P

P

P

S

G

G

S

S

F

V

K
|
K

I

M

R

R

G

G

I

I

G

G

H

A

A

F

C

C

-

Y

E

Q

T

A

Y

V

L

Q

A

T

R

R

G

G

A

T

R

N

-

I

R

Q

F

F

V

V

A

C

S

G

S

S

G
|
G

G

P

N
|
N

A

T

G

V

L

L

A

A

V

V

A

S

Y

Y

A

C

G

A

R

R

R

Q

L

L

G

G

V

V

P

E

V

A

T

I

V

I

V

V

P

P

E

K

T

A

T

T

T

N

E

E

R

R

A

I

K

C

K

Q

L

S

L

I

R

R

L

T

E

D

T

G

A

S

E

H

V

L

I

I

V

L

H

Y

G

G

A

E

S

N

W

W

Q

T

E

A

A

A

N

E

A

V

F

H

A

A

Q

R

S

K

L

L

I

V

G

R

A

R

S

N

D

G

A

I

F

Y

L

V

H

P

P

S

F
x
S

D

D

D

H

P

A

L

L

L

I

W

W

Q

Q

G

G

H

H

A

S

T

T

L

I

I

V

D

Q

E

E

V

L

F

K

C

T

-

Q

-

L

S

N

G

D

L

N

K

P

P

P

D

A

A

A

V

I

L

C

S
x
P

V

V

G
|
G

L

L

S

N

G

G

V

V

V

I

E

M

G

G

L

L

H

Q

R

E

N

A

G

D

W

W

S

K

D

D

I

V

P

P

V

V

F

I

A

A

V

V

E
|
E

T

T

T

H

G

G

A
x
S

A

N

S
x
A

F

F

H

Q

A

A

A

S

V

V

R

V

A

A

G

G

H

E

T

L

V

V

E

I

L

M

E

E

K

K

I

N

S

T

V

I

A

A

T

T

S
|
S

L

L

G

I

A

S

K

K

R

A

V

V

C

S

E

S

K

K

A

S

F

L

Q

E

W

L

S

S

K

L

N

N

H

H

P

P

I

V

H

V

S

P

V

F

V

A

V

V

S

S

D

D

R

A

S

M

A

A

L

A

S

D

A

A

C

V

E

R

R

L

F

F

L

A

A

E

D

D

H

F

R

K

I

M

L

L

V

V

E

E

P

A

A

S

C

A

G

G

A

A

S

A

L

L

A

S

I

L

A

C

Y

Y

E

T

N

H

-

L

-

I

-

R

-

D

-

I

A

L

S

P

E

S

L

L

E

S

N

P

F

A

S

K

N

D

I

V

L

V

V

V

V

L

V

V

C
x
T

G

G

A

S

T

D

T

I

T

S

I

L

D

A

Q

H

I

L

R

D

E

E

W

Y

active site: K37 (= K37), G63 (= G61), E191 (= E187), S195 (≠ A191), A197 (≠ S193), S219 (= S215), T297 (≠ C288)
binding pyridoxal-5'-phosphate: K37 (= K37), N65 (= N63), S132 (≠ F130), P163 (≠ S159), G165 (= G161), G166 (= G162), G167 (= G163), G168 (= G164), S219 (= S215), T297 (≠ C288)

P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
30% identity, 85% coverage: 7:268/308 of query aligns to 32:294/514 of P04968

query
sites
P04968

T

T

P

P

L

L

I

Q

E

K

S

M

R

E

A

K

M

L

S

S

V

S

M

R

T

L

G

D

R

N

S

V

V

I

W

L

I

V

K

K

L

R

D

E

A

D

L

R

Q

Q

P

P

P

V

G

H

S

S

F

F

K
|
K

I

L

R

R

G

G

-

A

-

Y

-

A

-

M

I

M

G

A

H

G

A

L

C

T

E

E

T

E

Y

Q

L

K

A

A

R

H

G

G

A

V

R

-

F

-

V

I

A

T

S

A

S

S

G

A

G

G

N
|
N

A

H

G

A

L

Q

A

G

V

V

A

A

Y

F

A

S

G

S

R

A

R

R

L

L

G

G

V

V

P

K

V

A

T

L

V

I

V

V

V

M

P

P

E

T

T

A

T

T

T

A

E

D

R

I

A

K

K

V

K

D

L

A

L

V

R

R

L

G

E

F

T

G

A

G

E

E

V

V

I

L

V

L

H

H

G

G

A

A

S

N

W

F

Q

D

E

E

A

A

N

K

A

A

F

K

A

A

Q

I

S

E

L

L

I

S

G

Q

A

Q

S

Q

D

G

-

F

A

T

F

W

L

V

H

P

P

P

F

F

D

D

D

H

P

P

L

M

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

L

I

A

D

L

E

E

V

L

F

L

C

Q

S

Q

G

D

L

A

K

H

P

L

D

D

A

R

V

V

V

F

L

V

S

P

V

V

G
|
G

L
|
L

L

A

S

A

G

G

V

V

V

A

E

V

G

L

L

I

H

-

R

K

N

Q

G

L

W

M

S

P

D

Q

I

I

P

K

V

V

F

I

A

A

V

V

E

E

T

A

T

E

G

D

A

S

A

A

S

C

F

L

H

K

A

A

V

L

R

D

A

A

G

G

H

H

T

P

V

V

E

D

L

L

E

P

K

R

I

V

I

G

S

L

V

F

A

A

T

E

S

G

L

V

G

A

A

V

K

K

R

R

V

I

C

G

E

D

K

E

A

T

F

F

Q

R

W

L

S

C

K

Q

N

E

H

Y

P

L

I

D

H

D

S

I

V

I

V

T

V

V

S

D

D

S

R

D

S

A

A

I

L

C

S

A

A

A

C

M

E

K

R

D

F

L

L

F

A

E

D

D

H

V

R

R

I

A

L

V

V

A

E

E

P

P

A

S

C

G

G

A

A

L

S

A

L

L

A

A

K62 (= K37) modified: N6-(pyridoxal phosphate)lysine
N89 (= N63) binding
GGGGL 188:192 (= GGGGL 161:165) binding

Sites not aligning to the query:

315 binding

7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
32% identity, 55% coverage: 2:169/308 of query aligns to 19:185/310 of 7nbgDDD

query
sites
7nbgDDD

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P

K

P

T

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

-

L

L

A

V

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A

T

S

H

S

S

G
x
S

G

G

N
|
N

A

H

G

G

L
x
Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V
x
I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A

K

K

K

K

L

L

A

L

I

R

Q

L

A

E
x
Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A

R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G
|
G

L
x
M

L

L

S

A

G

G

V

I

active site: K53 (= K37), S76 (≠ G61)
binding ortho-xylene: S76 (≠ G61), Q81 (≠ L66), I96 (≠ V81), Y113 (≠ E98)
binding pyridoxal-5'-phosphate: F52 (= F36), K53 (= K37), N78 (= N63), G177 (= G161), G178 (= G162), G179 (= G163), G180 (= G164), M181 (≠ L165)

Sites not aligning to the query:

active site: 202, 206, 208, 231, 304, 305
binding calcium ion: 202, 206, 208
binding magnesium ion: 3, 239
binding pyridoxal-5'-phosphate: 231, 232, 275, 277, 305, 306

1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
30% identity, 85% coverage: 7:268/308 of query aligns to 28:290/494 of 1tdjA

query
sites
1tdjA

T

T

P

P

L

L

I

Q

E

K

S

M

R

E

A

K

M

L

S

S

V

S

M

R

T

L

G

D

R

N

S

V

V

I

W

L

I

V

K

K

L

R

D

E

A

D

L

R

Q

Q

P

P

P

V

G

H

S

S

F
|
F

K
|
K

I

L

R

R

G

G

-

A

-

Y

-

A

-

M

I

M

G

A

H

G

A

L

C

T

E

E

T

E

Y

Q

L

K

A

A

R

H

G

G

A

V

R

-

F

-

V

I

A

T

S

A

S

S

G
x
A

G

G

N
|
N

A

H

G

A

L

Q

A

G

V

V

A

A

Y

F

A

S

G

S

R

A

R

R

L

L

G

G

V

V

P

K

V

A

T

L

V

I

V

V

V

M

P

P

E

T

T

A

T

T

T

A

E

D

R

I

A

K

K

V

K

D

L

A

L

V

R

R

L

G

E

F

T

G

A

G

E

E

V

V

I

L

V

L

H

H

G

G

A

A

S

N

W

F

Q

D

E

E

A

A

N

K

A

A

F

K

A

A

Q

I

S

E

L

L

I

S

G

Q

A

Q

S

Q

D

G

-

F

A

T

F

W

L

V

H

P

P

P

F

F

D

D

D

H

P

P

L

M

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

L

I

A

D

L

E

E

V

L

F

L

C

Q

S

Q

G

D

L

A

K

H

P

L

D

D

A

R

V

V

V

F

L

V

S

P

V

V

G
|
G

L

L

L

A

S

A

G

G

V

V

V

A

E

V

G

L

L

I

H

-

R

K

N

Q

G

L

W

M

S

P

D

Q

I

I

P

K

V

V

F

I

A

A

V

V

E
|
E

T

A

T

E

G

D

A
x
S

A

A

S
x
C

F

L

H

K

A

A

V

L

R

D

A

A

G

G

H

H

T

P

V

V

E

D

L

L

E

P

K

R

I

V

I

G

S

L

V

F

A

A

T

E

S
x
G

L

V

G

A

A

V

K

K

R

R

V

I

C

G

E

D

K

E

A

T

F

F

Q

R

W

L

S

C

K

Q

N

E

H

Y

P

L

I

D

H

D

S

I

V

I

V

T

V

V

S

D

D

S

R

D

S

A

A

I

L

C

S

A

A

A

C

M

E

K

R

D

F

L

L

F

A

E

D

D

H

V

R

R

I

A

L

V

V

A

E
|
E

P

P

A

S

C

G

G

A

A

L

S

A

L

L

A

A

active site: K58 (= K37), A83 (≠ G61), E209 (= E187), S213 (≠ A191), C215 (≠ S193), G237 (≠ S215)
binding pyridoxal-5'-phosphate: F57 (= F36), K58 (= K37), N85 (= N63), G184 (= G161), G185 (= G162), G186 (= G163), G187 (= G164), G237 (≠ S215), E282 (= E260)

Sites not aligning to the query:

active site: 310, 311
binding pyridoxal-5'-phosphate: 311, 312

3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
32% identity, 55% coverage: 2:169/308 of query aligns to 20:186/322 of 3l6bA

query
sites
3l6bA

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P

K

P

T

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

-

L

L

A

V

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A

T

S

H

S
|
S

G
x
S

G

G

N
|
N

A
x
H

G

G

L

Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V

I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A

K

K

K

K

L

L

A

L

I

R

Q

L

A

E

Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A
x
R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G
|
G

L
x
M

L

L

S

A

G

G

V

I

active site: K54 (= K37), S77 (≠ G61)
binding malonate ion: K54 (= K37), S76 (= S60), S77 (≠ G61), N79 (= N63), H80 (≠ A64), R128 (≠ A112)
binding pyridoxal-5'-phosphate: F53 (= F36), K54 (= K37), N79 (= N63), G178 (= G161), G179 (= G162), G180 (= G163), G181 (= G164), M182 (≠ L165)

Sites not aligning to the query:

active site: 203, 207, 209, 232, 278, 305, 306
binding malonate ion: 232
binding manganese (ii) ion: 203, 207, 209
binding pyridoxal-5'-phosphate: 233, 276, 278, 306, 307

7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
29% identity, 65% coverage: 2:201/308 of query aligns to 19:221/322 of 7nbgAAA

query
sites
7nbgAAA

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P

K

P

T

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

L

-

V

-

P

-

D

-

A

L

L

A

E

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A

T

S

H

S

S

G
x
S

G

G

N
|
N

A

H

G
|
G

L
x
Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V
x
I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A
x
K

K

K

K

L

L

A

L
x
I

R

Q

L

A

E
x
Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A

R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G
|
G

L
x
M

L

L

S

A

G

G

V

I

-

A

-

I

-

T

V

V

E

K

G

A

L

L

H

K

R

-

N

-

G

-

W

-

S

P

D

S

I

V

P

K

V

V

F

Y

A

A

V

A

E
|
E

T

P

T

S

G

N

A
|
A

A

D

S
x
D

F

C

H

Y

A

Q

A

S

V

K

R

L

A

K

G

G

active site: K53 (= K37), S81 (≠ G61), E207 (= E187), A211 (= A191), D213 (≠ S193)
binding calcium ion: E207 (= E187), A211 (= A191), D213 (≠ S193)
binding pyridoxal-5'-phosphate: F52 (= F36), K53 (= K37), N83 (= N63), G182 (= G161), G183 (= G162), G184 (= G163), G185 (= G164), M186 (≠ L165)
binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ G61), G85 (= G65), Q86 (≠ L66), I101 (≠ V81), K111 (≠ A91), I115 (≠ L95), Y118 (≠ E98)

Sites not aligning to the query:

active site: 236, 309, 310
binding pyridoxal-5'-phosphate: 236, 237, 282, 310, 311

7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
29% identity, 65% coverage: 2:201/308 of query aligns to 19:221/323 of 7nbfAAA

query
sites
7nbfAAA

T

S

L

I

H
|
H

I
x
L

E

-

T
|
T

P
|
P

L

V

I
x
L

E
x
T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L
x
F

Q

Q

P

K

P

T

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

L

-

V

-

P

-

D

-

A

L

L

A

E

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A

T

S

H

S

S

G
x
S

G

G

N
|
N

A

H

G

G

L

Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V

I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A

K

K

K

K

L

L

A

L

I

R

Q

L

A

E

Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A

R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G
|
G

L
x
M

L

L

S

A

G

G

V

I

-

A

-

I

-

T

V

V

E

K

G

A

L

L

H

K

R

P

N

S

G

-

W

-

S

-

D

-

I

V

P

K

V

V

F

Y

A

A

V

A

E
|
E

T

P

T

S

G

N

A
|
A

A

D

S
x
D

F

C

H

Y

A

Q

A

S

V

K

R

L

A

K

G

G

active site: K53 (= K37), S81 (≠ G61), E207 (= E187), A211 (= A191), D213 (≠ S193)
binding calcium ion: E207 (= E187), A211 (= A191), D213 (≠ S193)
binding pyridoxal-5'-phosphate: F52 (= F36), K53 (= K37), N83 (= N63), G182 (= G161), G183 (= G162), G184 (= G163), G185 (= G164), M186 (≠ L165)
binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (= H4), L22 (≠ I5), T23 (= T7), P24 (= P8), L26 (≠ I10), T27 (≠ E11), F46 (≠ L30)

Sites not aligning to the query:

active site: 236, 309, 310
binding magnesium ion: 3, 244
binding pyridoxal-5'-phosphate: 236, 237, 282, 310, 311

7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
29% identity, 65% coverage: 2:201/308 of query aligns to 19:221/323 of 7nbdAAA

query
sites
7nbdAAA

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P

K

P

T

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

L

-

V

-

P

-

D

-

A

L

L

A

E

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A

T

S

H

S

S

G
x
S

G

G

N
|
N

A

H

G

G

L

Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V

I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A

K

K

K

K

L

L

A

L

I

R

Q

L

A

E

Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A

R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G
|
G

L
x
M

L

L

S

A

G

G

V

I

-

A

-

I

-

T

V

V

E

K

G

A

L

L

H

K

R

P

N

S

G

-

W

-

S

-

D

-

I

V

P

K

V

V

F

Y

A

A

V

A

E
|
E

T

P

T

S

G

N

A
|
A

A

D

S
x
D

F

C

H

Y

A

Q

A

S

V

K

R

L

A

K

G

G

active site: K53 (= K37), S81 (≠ G61), E207 (= E187), A211 (= A191), D213 (≠ S193)
binding calcium ion: E207 (= E187), A211 (= A191), D213 (≠ S193)
binding pyridoxal-5'-phosphate: F52 (= F36), K53 (= K37), N83 (= N63), G182 (= G161), G183 (= G162), G184 (= G163), G185 (= G164), M186 (≠ L165)

Sites not aligning to the query:

active site: 236, 309, 310
binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: 272, 278, 314, 316
binding magnesium ion: 3, 244
binding pyridoxal-5'-phosphate: 236, 237, 280, 282, 310, 311

7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
29% identity, 65% coverage: 2:201/308 of query aligns to 19:221/323 of 7nbcCCC

query
sites
7nbcCCC

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P

K

P

T

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

L

-

V

-

P

-

D

-

A

L

L

A

E

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A
x
T

S
x
H

S

S

G
x
S

G

G

N
|
N

A
x
H

G

G

L

Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V

I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A

K

K

K

K

L

L

A

L

I

R

Q

L

A

E

Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A

R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-
x
V

H
|
H

P
|
P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G
|
G

L
x
M

L

L

S

A

G

G

V

I

-

A

-

I

-

T

V

V

E

K

G

A

L

L

H

K

R

P

N

S

G

-

W

-

S

-

D

-

I

V

P

K

V

V

F

Y

A

A

V

A

E
|
E

T

P

T

S

G

N

A
|
A

A

D

S
x
D

F

C

H

Y

A

Q

A

S

V

K

R

L

A

K

G

G

active site: K53 (= K37), S81 (≠ G61), E207 (= E187), A211 (= A191), D213 (≠ S193)
binding biphenyl-4-ylacetic acid: T78 (≠ A58), H79 (≠ S59), H84 (≠ A64), V148 (vs. gap), H149 (= H128), P150 (= P129)
binding calcium ion: E207 (= E187), A211 (= A191), D213 (≠ S193)
binding pyridoxal-5'-phosphate: F52 (= F36), K53 (= K37), N83 (= N63), G182 (= G161), G183 (= G162), G184 (= G163), G185 (= G164), M186 (≠ L165)

Sites not aligning to the query:

active site: 236, 309, 310
binding pyridoxal-5'-phosphate: 236, 237, 282, 310, 311

7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
29% identity, 65% coverage: 2:201/308 of query aligns to 19:221/323 of 7nbcAAA

query
sites
7nbcAAA

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P

K

P

T

G

G

S

S

F
|
F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

L

-

V

-

P

-

D

-

A

L

L

A

E

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A

T

S

H

S

S

G
x
S

G

G

N
|
N

A

H

G

G

L

Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V

I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A

K

K

K

K

L

L

A

L

I

R

Q

L

A

E

Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A

R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G
|
G

L
x
M

L

L

S

A

G

G

V

I

-

A

-

I

-

T

V

V

E

K

G

A

L

L

H

K

R

P

N

S

G

-

W

-

S

-

D

-

I

V

P

K

V

V

F

Y

A

A

V

A

E
|
E

T

P

T

S

G

N

A
|
A

A

D

S
x
D

F

C

H

Y

A

Q

A

S

V

K

R

L

A

K

G

G

active site: K53 (= K37), S81 (≠ G61), E207 (= E187), A211 (= A191), D213 (≠ S193)
binding calcium ion: E207 (= E187), A211 (= A191), D213 (≠ S193)
binding pyridoxal-5'-phosphate: F52 (= F36), K53 (= K37), N83 (= N63), G182 (= G161), G183 (= G162), G184 (= G163), G185 (= G164), M186 (≠ L165)

Sites not aligning to the query:

active site: 236, 309, 310
binding magnesium ion: 3, 244
binding pyridoxal-5'-phosphate: 236, 237, 282, 310, 311

7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
29% identity, 65% coverage: 2:201/308 of query aligns to 19:221/320 of 7nbhAAA

query
sites
7nbhAAA

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S

S

R

S

A

I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P

K

P

T

G

G

S

S

F

F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

L

-

V

-

P

-

D

-

A

L

L

A

E

R

R

G

K

A

P

R

K

R

A

F

V

V

V

A

T

S

H

S

S

G
x
S

G

G

N

N

A

H

G
|
G

L
x
Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V

I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A
x
K

K

K

K

L

L

A

L
x
I

R

Q

L

A

E
x
Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A

R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G

G

L

M

L

L

S

A

G

G

V

I

-

A

-

I

-

T

V

V

E

K

G

A

L

L

H

K

R

P

N

S

G

-

W

-

S

-

D

-

I

V

P

K

V

V

F

Y

A

A

V

A

E
|
E

T

P

T

S

G

N

A
|
A

A

D

S
x
D

F

C

H

Y

A

Q

A

S

V

K

R

L

A

K

G

G

active site: K53 (= K37), S81 (≠ G61), E207 (= E187), A211 (= A191), D213 (≠ S193)
binding calcium ion: E207 (= E187), A211 (= A191), D213 (≠ S193)
binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ G61), G85 (= G65), Q86 (≠ L66), K111 (≠ A91), I115 (≠ L95), Y118 (≠ E98)

Sites not aligning to the query:

active site: 236, 309, 310
binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: 235, 281, 313, 314, 315

6zspAAA serine racemase bound to atp and malonate. (see paper)
29% identity, 65% coverage: 2:201/308 of query aligns to 19:214/320 of 6zspAAA

query
sites
6zspAAA

T

S

L

I

H

H

I

L

E

-

T

T

P

P

L

V

I

L

E

T

S
|
S

R
x
S

A
x
I

M

L

S

N

V

Q

M

L

T

T

G

G

R

R

S

N

V

L

W

F

I

F

K

K

L

C

D

E

A

L

L

F

Q

Q

P
x
K

P
x
T

G

G

S

S

F

F

K
|
K

I

I

R

R

G

G

I

A

G

L

H

N

A

A

C

V

E

R

T

S

Y

L

L

K

A

P

R

K

G

A

A

-

R

-

F

V

V

V

A

T

S

H

S
|
S

G
x
S

G

G

N
|
N

A
x
H

G

G

L
x
Q

A

A

V

L

A

T

Y

Y

A

A

G

A

R

K

R

L

L

E

G

G

V

I

P

P

V

A

T

Y

V

I

V

V

P

P

E

Q

T

T

T

A

T

P

E

D

R

C

A

K

K

K

K

L

L

A

L

I

R

Q

L

A

E
x
Y

T

G

A

A

E

S

V

I

I

V

V

Y

H

C

G

E

A

P

S

S

W

D

Q

E

E

S

A
x
R

N

E

A

N

F

V

A

A

Q

K

S

R

L

V

I

T

G

E

A

E

S

T

D

E

A

G

F

I

L

M

-

V

H

H

P

P

F

N

D

Q

D

E

P

P

L

A

L

V

W

I

Q

A

G

G

H

Q

A

G

T

T

L

I

I

A

D

L

E

E

V

V

F

L

C

N

S

Q

G

V

L

P

K

L

P

V

D

D

A

A

V

L

V

V

L

V

S

P

V

V

G

G

L

M

L

L

S

A

G

G

V

I

-

A

-

I

-

T

V

V

E

K

G

A

L

L

H

K

R

-

N

-

G

-

W

-

S

P

D

S

I

V

P

K

V

V

F

Y

A

A

V

A

E
|
E

T

P

T

S

G

N

A
|
A

A

D

S
x
D

F

C

H

Y

A

Q

A

S

V

K

R

L

A

K

G

G

active site: K53 (= K37), S74 (≠ G61), E200 (= E187), A204 (= A191), D206 (≠ S193)
binding adenosine-5'-triphosphate: S28 (= S12), S29 (≠ R13), I30 (≠ A14), K48 (≠ P32), T49 (≠ P33), Q79 (≠ L66), Y111 (≠ E98)
binding magnesium ion: E200 (= E187), A204 (= A191), D206 (≠ S193)
binding malonate ion: K53 (= K37), S73 (= S60), S74 (≠ G61), N76 (= N63), H77 (≠ A64), R125 (≠ A112)

Sites not aligning to the query:

active site: 229, 302, 303
binding adenosine-5'-triphosphate: 266, 267, 269, 306
binding malonate ion: 229, 232

A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
26% identity, 85% coverage: 7:268/308 of query aligns to 39:304/360 of A0R220

query
sites
A0R220

T

T

P

P

L

L

I

I

E

H

S

A

R

K

A

R

M

I

S

S

V

E

M

L

T

T

G

G

R

C

S

T

V

V

W

H

I

L

K

K

L

V

D

E

A

G

L

L

Q

N

P

P

P

T

G

G

S

S

F

F

K

K

I

D

R

R

G

G

I

M

G

T

H

V

A

A

C

V

E

T

T

E

Y

S

L

L

A

A

R

R

G

G

A

Q

R

Q

R

A

F

V

V

L

A

C

S

A

S

S

G

T

G

G

N

N

A

T

G

S

L

A

A

S

V

A

A

A

Y

A

A

Y

G

A

R

A

R

R

L

A

G

G

V

I

P

T

V

C

T

A

V

V

V

L

V

I

P

P

E

Q

T

-

E

-

R

-

A

-

K

G

K

K

L

I

L

A

R

M

L

G

E

K

T

L

A

A

E

Q

V

A

I

V

V

M

H

H

G

G

A

A

S

K

W

I

Q

I

E

Q

A

V

N

D

A

G

F

N

A

F

Q

D

S

D

L

C

I

L

G

E

A

L

S

A

D

R

A
x
K

F

L

L

T

H

A

P

D

F

F

D

P

-

T

-

I

-

A

-

L

-

V

-

N

-

S

-

V

D

N

P

P

L

Y

L

R

W

I

Q

E

G

G

H

Q

A

K

T

T

L

A

I

A

D

F

E

E

V

I

F

V

C

D

S

A

-

L

G

G

L

T

K

A

P

P

D

D

A

V

V

H

V

A

L

L

S

P

V

V

G

G

G

N

G

A

G

G

L

N

L

I

S

T

G

A

V

Y

V

W

E

K

G

G

-

Y

-

S

-

E

L

Y

H

H

R

R

N

D

G

G

W

V

S

S

D

D

-

R

I

L

P

P

-

R

V

M

F

L

A

G

V

T

E

Q

T

A

G

G

A

A

S

P

F

L

H

-

A

-

V

-

R

V

A

T

G

G

H

A

T

P

V

V

E

K

L

D

E

P

K

E

I

T

I

I

S

A

V

T

A

A

T

I

S

R

L

I

G

G

A

S

K

P

R

A

V

S

C

W

E

N

K

S

A

A

F

V

Q

E

W

A

S

Q

K

Q

N

Q

H

S

P

D

I

G

H

R

S

F

V

L

V

A

S

T

D

D

R

E

S

E

A

I

L

L

S

A

A

A

C

Y

E

H

R

L

F

V

L

A

A

R

D

T

H

E

R

G

I

V

L

F

V

V

E

E

P

P

A

A

C

S

G

A

A

A

S

S

L

I

A

A

K151 (≠ A125) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Query Sequence

>WP_086511696.1 NCBI__GCF_002151265.1:WP_086511696.1
MTLHIETPLIESRAMSVMTGRSVWIKLDALQPPGSFKIRGIGHACETYLARGARRFVASS
GGNAGLAVAYAGRRLGVPVTVVVPETTTERAKKLLRLETAEVIVHGASWQEANAFAQSLI
GASDAFLHPFDDPLLWQGHATLIDEVFCSGLKPDAVVLSVGGGGLLSGVVEGLHRNGWSD
IPVFAVETTGAASFHAAVRAGHTVELEKIISVATSLGAKRVCEKAFQWSKNHPIHSVVVS
DRSALSACERFLADHRILVEPACGASLAIAYENASELENFSNILVVVCGGATTTIDQIRE
WESSSQAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory