SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086511825.1 NCBI__GCF_002151265.1:WP_086511825.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

7bkbE Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
28% identity, 19% coverage: 312:403/489 of query aligns to 288:377/411 of 7bkbE

query
sites
7bkbE

D

E

T

S

L

S

R

K

C
|
C

I
|
I

R
x
K

C
|
C

G
x
Y

A
|
A

C
|
C

M

V

N

E

H

A

C
|
C

P
|
P

V

I

Y
x
C

T

Y

R
x
C

V

I

G

E

G
x
C

H
x
S

T

T

Y

K

G

K

T

P

T

W

Y

Y

-

I

-

A

P

P

G

G

P

V

I

L

G

P

S

T

I

S

L

F

M

M

P
x
F

H

H

M

L

M

I

G

R

L

F

E

A

A
x
H

T

V

K

S

D

D

L

-

P

-

T

-

A

S

S
x
C

S
x
I

L
x
N

C
|
C

G
|
G

A

Q

C
|
C

G

E

E

E

V

L

C
|
C

P
|
P

V

M

K

E

I

I

P

P

I

-

P
x
N

D

A

L

L

L

F

V
x
M

R

H

L

S

R

Q

E

V

A

E

V

I

G

E

E

K

G

M

R

F

G

G

N

H

V

I

P

P

G

G

binding iron/sulfur cluster: C292 (= C316), I293 (= I317), K294 (≠ R318), C295 (= C319), Y296 (≠ G320), A297 (= A321), C298 (= C322), C302 (= C326), P303 (= P327), C305 (≠ Y329), C307 (≠ R331), C310 (≠ G334), S311 (≠ H335), F329 (≠ P351), H336 (≠ A358), C341 (≠ S366), I342 (≠ S367), N343 (≠ L368), C344 (= C369), G345 (= G370), C347 (= C372), C351 (= C376), P352 (= P377), N357 (≠ P383), M361 (≠ V387)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 20, 21, 22, 24, 26, 27, 45, 68, 69, 70, 71, 72, 73, 75, 98, 127, 128, 129, 205, 206, 207, 218
binding iron/sulfur cluster: 101, 102, 128, 189, 192

Query Sequence

>WP_086511825.1 NCBI__GCF_002151265.1:WP_086511825.1
MSASEVQTFTPREFHRQAHDALGNPQIRANFRKAMDGLMAKRRDVFSDWDLETLRELGAN
IRLRALAKLPDLLERLEANCQANGIQVHWAENGDEACRIIREICQRHGAKAVIKGKSMVS
EEMHLNAHLEEAGIEALESDLGEYLVQLNEQTPSHIIMPAIHLNTDEISEIMHTRTGTER
TRDVDTMTAAARAQLRERFMAADVGISGVNFAVAETGTLCLVENEGNGRMTTAVPPVHIA
VTGIEKVVEHLRDVPPLYALLTRSATGQHVTTYFNMISSPRKLGEHDGPNEVHLVLVDSG
RSNIYQDDELLDTLRCIRCGACMNHCPVYTRVGGHTYGTTYPGPIGSILMPHMMGLEATK
DLPTASSLCGACGEVCPVKIPIPDLLVRLRREAVGEGRGNVPGAGVKRTKKEVAAWKGFQ
WLATHPGAWRSSTAIAGKLQALMPSKLGPWTEYRTAPKPAKVSLHALVKQHKAKRHQAGK
SDEDGGKRS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory