SitesBLAST
Comparing WP_086511882.1 NCBI__GCF_002151265.1:WP_086511882.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4e5kA Thermostable phosphite dehydrogenase in complex with NAD and sulfite (see paper)
51% identity, 97% coverage: 1:320/331 of query aligns to 1:325/329 of 4e5kA
- active site: L100 (= L100), R237 (= R232), D261 (= D256), E266 (= E261), H292 (= H287)
- binding nicotinamide-adenine-dinucleotide: K76 (= K76), G77 (= G77), L100 (= L100), T104 (= T104), G152 (= G152), G154 (= G154), A155 (≠ R155), I156 (≠ L156), H174 (vs. gap), E175 (vs. gap), A176 (= A170), A207 (≠ L202), L208 (= L203), P209 (= P204), P235 (≠ V230), C236 (≠ G231), R237 (= R232), D261 (= D256), H292 (= H287), G294 (= G289)
- binding sulfite ion: M53 (= M53), L75 (= L75), K76 (= K76), G77 (= G77), L100 (= L100), R237 (= R232), H292 (= H287)
4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
51% identity, 97% coverage: 1:320/331 of query aligns to 1:325/332 of 4e5pA
- active site: L100 (= L100), R237 (= R232), D261 (= D256), E266 (= E261), H292 (= H287)
- binding nicotinamide-adenine-dinucleotide: K76 (= K76), L100 (= L100), T104 (= T104), G154 (= G154), A155 (≠ R155), I156 (≠ L156), A175 (vs. gap), R176 (vs. gap), L208 (= L203), P209 (= P204), T214 (= T209), P235 (≠ V230), C236 (≠ G231), R237 (= R232), H292 (= H287)
4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
51% identity, 97% coverage: 1:320/331 of query aligns to 1:325/329 of 4e5mA
- active site: L100 (= L100), R237 (= R232), D261 (= D256), E266 (= E261), H292 (= H287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (= K76), L100 (= L100), T104 (= T104), G154 (= G154), A155 (≠ R155), I156 (≠ L156), R176 (vs. gap), L208 (= L203), P209 (= P204), T214 (= T209), P235 (≠ V230), C236 (≠ G231), R237 (= R232), H292 (= H287), G294 (= G289)
6ih6A Phosphite dehydrogenase mutant i151r/p176r/m207a from ralstonia sp. 4506 in complex with non-natural cofactor nicotinamide cytosine dinucleotide
47% identity, 97% coverage: 1:322/331 of query aligns to 1:328/330 of 6ih6A
- binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: T104 (= T104), R151 (≠ L151), G154 (= G154), A155 (≠ R155), V156 (≠ L156), D175 (≠ A170), A207 (≠ L202), V208 (≠ L203), P209 (= P204), T214 (= T209), A235 (≠ V230), C236 (≠ G231), R237 (= R232)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
34% identity, 99% coverage: 1:327/331 of query aligns to 1:328/334 of 5aovA
- active site: L100 (= L100), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H287)
- binding glyoxylic acid: M52 (≠ F52), L53 (≠ M53), L53 (≠ M53), Y74 (≠ A74), A75 (≠ L75), V76 (≠ K76), G77 (= G77), R241 (= R232), H288 (= H287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ K76), T104 (= T104), F158 (≠ L153), G159 (= G154), R160 (= R155), I161 (≠ L156), S180 (≠ V175), R181 (≠ D176), A211 (≠ L202), V212 (≠ L203), P213 (= P204), T218 (= T209), I239 (≠ V230), A240 (≠ G231), R241 (= R232), H288 (= H287), G290 (= G289)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
34% identity, 95% coverage: 4:316/331 of query aligns to 3:316/332 of 6biiA
- active site: L99 (= L100), R240 (= R232), D264 (= D256), E269 (= E261), H287 (= H287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ K76), T103 (= T104), G156 (= G152), F157 (≠ L153), G158 (= G154), R159 (= R155), I160 (≠ L156), A179 (= A170), R180 (= R171), S181 (≠ I172), K183 (≠ G174), V211 (≠ L203), P212 (= P204), E216 (= E208), T217 (= T209), V238 (= V230), A239 (≠ G231), R240 (= R232), D264 (= D256), H287 (= H287), G289 (= G289)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
35% identity, 99% coverage: 1:327/331 of query aligns to 1:328/333 of 2dbqA
- active site: L100 (= L100), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ K76), T104 (= T104), L158 (= L153), G159 (= G154), R160 (= R155), I161 (≠ L156), S180 (≠ G174), R181 (≠ V175), T182 (≠ D176), A211 (≠ L202), V212 (≠ L203), P213 (= P204), T218 (= T209), I239 (≠ V230), A240 (≠ G231), R241 (= R232), D265 (= D256), H288 (= H287), G290 (= G289)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
35% identity, 99% coverage: 1:327/331 of query aligns to 1:328/334 of O58320
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
32% identity, 94% coverage: 4:313/331 of query aligns to 8:319/328 of Q9UBQ7
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
32% identity, 94% coverage: 4:313/331 of query aligns to 4:315/324 of 2gcgA
- active site: L103 (= L100), R241 (= R232), D265 (= D256), E270 (= E261), H289 (= H287)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (≠ M53), S78 (≠ L75), V79 (≠ K76), G80 (= G77), R241 (= R232), H289 (= H287)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (≠ K76), T107 (= T104), G156 (= G152), G158 (= G154), I160 (≠ L156), G180 (≠ D176), R181 (≠ T177), R184 (≠ S180), C212 (≠ L203), S213 (≠ P204), T218 (= T209), I239 (≠ V230), R241 (= R232), D265 (= D256), H289 (= H287), G291 (= G289)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
31% identity, 82% coverage: 42:314/331 of query aligns to 42:306/313 of Q65CJ7
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
31% identity, 82% coverage: 42:314/331 of query aligns to 40:304/311 of 3bazA
- active site: L98 (= L100), R230 (= R232), A251 (≠ Y253), D254 (= D256), E259 (≠ L269), H277 (= H287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ K76), G149 (= G152), L150 (= L153), G151 (= G154), R152 (= R155), I153 (≠ L156), S172 (≠ A170), R173 (= R171), S174 (≠ I172), C201 (≠ L203), P202 (= P204), T207 (= T209), I228 (≠ V230), G229 (= G231), R230 (= R232), D254 (= D256), H277 (= H287), G279 (= G289)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
35% identity, 83% coverage: 45:320/331 of query aligns to 42:311/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
35% identity, 83% coverage: 45:320/331 of query aligns to 41:310/526 of 3dc2A
Sites not aligning to the query:
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
29% identity, 83% coverage: 56:331/331 of query aligns to 58:327/533 of O43175
- T78 (≠ K76) binding
- R135 (= R135) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ RL 155:156) binding
- D175 (vs. gap) binding
- T207 (≠ L203) binding
- CAR 234:236 (≠ VGR 230:232) binding
- D260 (= D256) binding
- V261 (= V257) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HLGS 287:290) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
31% identity, 73% coverage: 56:296/331 of query aligns to 52:286/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ L100), A100 (≠ T104), R149 (= R155), I150 (≠ L156), Y168 (vs. gap), D169 (vs. gap), P170 (vs. gap), I171 (vs. gap), H200 (≠ L202), T201 (≠ L203), P202 (= P204), T207 (= T209), C228 (≠ V230), A229 (≠ G231), R230 (= R232), H277 (= H287), G279 (= G289)
7dkmA Phgdh covalently linked to oridonin (see paper)
31% identity, 73% coverage: 56:296/331 of query aligns to 54:288/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ K76), A102 (≠ T104), G148 (= G152), R151 (= R155), I152 (≠ L156), Y170 (vs. gap), D171 (vs. gap), P172 (vs. gap), I173 (vs. gap), H202 (≠ L202), T203 (≠ L203), P204 (= P204), T209 (= T209), C230 (≠ V230), A231 (≠ G231), R232 (= R232), H279 (= H287), G281 (= G289)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
31% identity, 73% coverage: 56:296/331 of query aligns to 50:284/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G154), I148 (≠ L156), Y166 (vs. gap), D167 (vs. gap), P168 (vs. gap), I169 (vs. gap), I170 (≠ L173), H198 (≠ L202), T199 (≠ L203), L208 (= L212), R228 (= R232)
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
31% identity, 73% coverage: 56:296/331 of query aligns to 54:288/305 of 6plfA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
31% identity, 73% coverage: 56:296/331 of query aligns to 53:287/301 of 6rj5A
Query Sequence
>WP_086511882.1 NCBI__GCF_002151265.1:WP_086511882.1
MTDKILITHPVHEDVYHRLAAVAEVDMNPDLEPWPYAEVCRRAADATAIMGFMTDRVDTE
LLTNAPRLKIIACALKGYDNYDAEACAQAGVWLSIVPDLLTAPTAELAVALALGLARHVR
AGDAFVRQGNYRGWRPHFYGYGLHNSTVAVLGLGRLGSALVERLQGFGCARILGVDTHAS
LPGVVNCSLQDALAQADFVFSLLPLSEETWHLLDAAHLRACKPGQLLINVGRGSVVDELA
VADVLAEGRLGGYAADVFSCEDWAYLERLRKIPDALLLAENTLFTPHLGSAVHSARRAIE
HRAADNILAVLAGRAPEDAVAEAEIRLAVCP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory