SitesBLAST
Comparing WP_087506884.1 NCBI__GCF_002165625.1:WP_087506884.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1vj0A Crystal structure of alcohol dehydrogenase (tm0436) from thermotoga maritima at 2.00 a resolution
30% identity, 97% coverage: 13:369/369 of query aligns to 10:366/366 of 1vj0A
- active site: C42 (= C41), G43 (= G42), S44 (≠ T43), H47 (= H46), H64 (= H62), E65 (= E63), C99 (= C97), C102 (= C100), C105 (= C103), C114 (= C113), K118 (≠ G117), C165 (≠ V163), T169 (≠ V165), K360 (= K363)
- binding zinc ion: C42 (= C41), H64 (= H62), E65 (= E63), C99 (= C97), C102 (= C100), C105 (= C103), C114 (= C113), C165 (≠ V163)
7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
29% identity, 94% coverage: 9:354/369 of query aligns to 7:341/357 of 7y9pA
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
29% identity, 94% coverage: 8:354/369 of query aligns to 5:330/348 of 1e3jA
- active site: C38 (= C41), G39 (= G42), S40 (≠ T43), H43 (= H46), H63 (= H62), E64 (= E63), C93 (= C97), C96 (= C100), C99 (= C103), C107 (= C113), T111 (≠ G120), P150 (= P162), G154 (≠ A166)
- binding phosphate ion: A174 (= A190), A196 (≠ D213), R197 (≠ G214), S198 (≠ Q215), R201 (= R218)
- binding zinc ion: C38 (= C41), H63 (= H62), E64 (= E63), C93 (= C97), C96 (= C100), C99 (= C103), C107 (= C113)
Sites not aligning to the query:
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
29% identity, 91% coverage: 23:357/369 of query aligns to 21:332/347 of 5vm2A
- active site: C39 (= C41), G40 (= G42), S41 (≠ T43), H44 (= H46), H65 (= H62), E66 (= E63), C95 (= C97), C98 (= C100), C101 (= C103), C109 (= C113), D113 (≠ G120), P153 (= P162), G157 (≠ A166)
- binding magnesium ion: H65 (= H62), E66 (= E63), E152 (= E161)
- binding zinc ion: C95 (= C97), C98 (= C100), C101 (= C103), C109 (= C113)
Sites not aligning to the query:
3qe3A Sheep liver sorbitol dehydrogenase (see paper)
29% identity, 95% coverage: 16:365/369 of query aligns to 14:341/351 of 3qe3A
- active site: C39 (= C41), G40 (= G42), S41 (≠ T43), H44 (= H46), H64 (= H62), E65 (= E63), R94 (≠ K89), D97 (≠ T92), C100 (≠ I95), S108 (≠ G101), F112 (vs. gap), P151 (= P162), G155 (≠ A166), K339 (= K363)
- binding glycerol: Y45 (≠ E47), F54 (≠ I56), T116 (≠ S105), R293 (≠ A313)
- binding zinc ion: C39 (= C41), H64 (= H62), E65 (= E63)
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
28% identity, 95% coverage: 16:365/369 of query aligns to 20:347/357 of P27867
- C45 (= C41) binding Zn(2+)
- H70 (= H62) binding Zn(2+)
- E71 (= E63) binding Zn(2+)
- E156 (= E161) binding Zn(2+)
- D204 (= D213) binding NAD(+)
- R209 (= R218) binding NAD(+)
- VGM 273:275 (≠ LGF 284:286) binding NAD(+)
- VFR 297:299 (≠ SWA 311:313) binding NAD(+)
P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
30% identity, 95% coverage: 16:365/369 of query aligns to 18:344/354 of P07846
- C43 (= C41) binding Zn(2+)
- Y49 (≠ E47) binding substrate
- H67 (= H62) binding Zn(2+)
- E68 (= E63) binding Zn(2+)
- E153 (= E161) binding substrate
- R296 (≠ A313) binding substrate
- Y297 (= Y314) binding substrate
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
28% identity, 93% coverage: 22:365/369 of query aligns to 19:337/341 of P07913
- C38 (= C41) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
28% identity, 96% coverage: 10:365/369 of query aligns to 14:335/339 of 5kiaA
- active site: C37 (= C41), G38 (= G42), T39 (= T43), H42 (= H46), H61 (= H62), E62 (= E63), C91 (= C97), C94 (= C100), C97 (= C103), C105 (= C113), V109 (≠ G117), P147 (= P162), A151 (= A166), K333 (= K363)
- binding calcium ion: D146 (≠ E161), N150 (≠ V165), E288 (≠ S315)
- binding zinc ion: C91 (= C97), C94 (= C100), C97 (= C103), C105 (= C113)
Q7SI09 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 2 papers)
28% identity, 90% coverage: 25:355/369 of query aligns to 37:346/363 of Q7SI09
- C53 (= C41) binding Zn(2+)
- F59 (≠ E47) mutation F->A,S,Y: No effect.
- H78 (= H62) binding Zn(2+)
- E79 (= E63) binding Zn(2+)
- C108 (= C97) binding Zn(2+)
- C111 (= C100) binding Zn(2+)
- C114 (= C103) binding Zn(2+)
- C122 (= C113) binding Zn(2+)
- E163 (= E161) binding Zn(2+)
- PI 190:191 (= PI 192:193) binding NAD(+)
- D211 (= D213) binding NAD(+)
- DI 211:212 (≠ DG 213:214) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-348.
- R216 (= R218) binding NAD(+)
- I282 (≠ V288) binding NAD(+)
- QYR 306:308 (≠ SWA 311:313) binding NAD(+)
Sites not aligning to the query:
- 348 S→T: Alters cofactor specificity from NAD to NADP; when associated with 211-SR-212.
3m6iA L-arabinitol 4-dehydrogenase (see paper)
28% identity, 90% coverage: 25:355/369 of query aligns to 33:342/358 of 3m6iA
- active site: C49 (= C41), G50 (= G42), S51 (≠ T43), H54 (= H46), H74 (= H62), E75 (= E63), C104 (= C97), C107 (= C100), C110 (= C103), C118 (= C113), D122 (≠ G117), P160 (= P162), A164 (= A166)
- binding nicotinamide-adenine-dinucleotide: C49 (= C41), V163 (= V165), G185 (= G191), P186 (= P192), I187 (= I193), D207 (= D213), R212 (= R218), C255 (= C261), T256 (= T262), I278 (≠ V288), G279 (≠ D289), V280 (≠ N290), R304 (≠ A313)
- binding zinc ion: C49 (= C41), H74 (= H62), C104 (= C97), C107 (= C100), C110 (= C103), C118 (= C113)
Sites not aligning to the query:
A2QAC0 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) (see 2 papers)
28% identity, 98% coverage: 3:364/369 of query aligns to 8:368/386 of A2QAC0
- M70 (≠ I56) mutation to F: Abolishes enzyme activity.
- DI 213:214 (≠ DG 213:214) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-359.
- Y318 (≠ W312) mutation to F: Increases affinity for D-sorbitol.
- A359 (vs. gap) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 213-SR-214.
4oh1A Crystal structure of a putative zinc-binding dehydrogenase (gutb) from clostridium scindens atcc 35704 at 2.00 a resolution
30% identity, 79% coverage: 22:314/369 of query aligns to 27:305/351 of 4oh1A