SitesBLAST

S109 (≠ Q4) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
N127 (≠ Q25) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
G131 (= G29) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
I147 (≠ L43) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (≠ Y49) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (≠ T68) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (= P88) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (= A102) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (= A128) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
G233 (= G130) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
169:521 natural variant: Missing (in D2HGA1; uncertain significance)
375 D → Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
386 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; R→A: Loss of catalytic activity.
390 binding (R)-2-hydroxyglutarate; binding (R)-malate; T→A: Significantly reduced catalytic activity.
399 V → M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
401 binding (R)-2-hydroxyglutarate; binding (R)-malate; K→A: Loss of catalytic activity.
419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
434 binding Zn(2+); H→A: Loss of catalytic activity.
436 G → V: slight reduction in catalytic activity
439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
441 binding Zn(2+); H→A: Loss of catalytic activity.
443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
475 binding Zn(2+); E→A: Loss of catalytic activity.
476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
28% identity, 49% coverage: 2:175/357 of query aligns to 54:225/466 of 6lpxA

query
sites
6lpxA

Q

R

A

T

Q

S

Q

E

D

E

M

V

E

S

A

H

V

I

L

L

A

R

S

H

F

C

R

H

E

E

R

R

I

N

L

L

S

-

G

-

R

-

A

A

L

V

Q

N

I
x
P

R
x
Q

G
|
G

G
x
N

T
|
T

K
x
G

D
x
M

W

V

Y

G

G
|
G

Q
x
S

V

V

P

P

S

V

G

F

D

D

-

E

-

I

L

I

L

L

D

S

T

T

R

A

P

R

Y

M

S

N

G

R

I

V

I

L

D

S

Y

F

E

H

P

S

T

V

E

S

L

G

V

I

I

L

T

V

A

C

R

Q

C

A

G

G

T

C

P

V

L

L

A

E

E

E

I

L

D

S

A

R

A

Y

L

V

A

E

G

E

H

R

N

D

Q

F

M

I

L

M

A

-

W

-

E

-

P

P

P

L

R

D

F
x
L

G
|
G

-
x
A

-

K

G

G

A

S

A
x
C

T

H

V

I

G

G

G
|
G

M
x
N

V

V

A
|
A

A
x
T

G

N

L

A

S

G

G
|
G

P

L

S

R

R

F

A

L

A

R

A

Y

G

G

A

S

V

L

R

H

D

G

F

T

V

V

L

L

G

G

A

L

V

E

L

V

M

V

D

L

A

A

Q

D

G

G

E

T

Q

V

L

L

H

D

F

C

G

L

G

T

Q

S

V

L

M

R

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

I

L

S

K

R

Q

L

L

L

F

A

I

G

G

S

S

L

E

G
|
G

T

T

L

L

G

G

L

I

I
|
I

L

T

Q

T

V

V

S

S

L

I

K

L

V

C

L

P

P

P

R

K

P

P

I

R

A

A

binding flavin-adenine dinucleotide: P75 (≠ I26), Q76 (≠ R27), G77 (= G28), G78 (= G29), N79 (≠ G30), T80 (= T31), G81 (≠ K32), M82 (≠ D33), G85 (= G36), S86 (≠ Q37), L139 (≠ F91), G140 (= G92), A141 (vs. gap), C145 (≠ A95), G149 (= G99), N150 (≠ M100), A152 (= A102), T153 (≠ A103), G157 (= G107), G207 (= G157), I212 (= I162)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 333, 337, 348, 379, 381, 388, 423
binding flavin-adenine dinucleotide: 39, 422, 459
binding zinc ion: 381, 388, 422

6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
28% identity, 49% coverage: 2:175/357 of query aligns to 54:225/466 of 6lpuA

query
sites
6lpuA

Q

R

A

T

Q

S

Q

E

D

E

M

V

E

S

A

H

V

I

L

L

A

R

S

H

F

C

R

H

E

E

R

R

I

N

L

L

S

-

G

-

R

-

A

A

L

V

Q

N

I
x
P

R

Q

G
|
G

G
x
N

T
|
T

K
x
G

D

M

W

V

Y

G

G
|
G

Q
x
S

V

V

P

P

S

V

G

F

D

D

-

E

-

I

L

I

L

L

D

S

T

T

R

A

P

R

Y

M

S

N

G

R

I

V

I

L

D

S

Y

F

E

H

P

S

T

V

E

S

L

G

V

I

I

L

T

V

A

C

R

Q

C

A

G

G

T

C

P

V

L

L

A

E

E

E

I

L

D

S

A

R

A

Y

L

V

A

E

G

E

H

R

N

D

Q

F

M

I

L

M

A

-

W

-

E

-

P

P

P

L

R

D

F
x
L

G
|
G

-
x
A

-

K

G

G

A

S

A
x
C

T
x
H

V

I

G
|
G

M
x
N

V

V

A
|
A

A
x
T

G

N

L
x
A

S

G

G

G

P

L

S

R

R

F

A

L

A

R

A

Y

G

G

A

S

V

L

R

H

D

G

F

T

V

V

L

L

G

G

A

L

V

E

L

V

M

V

D

L

A

A

Q

D

G

G

E

T

Q

V

L

L

H

D

F

C

G

L

G

T

Q

S

V

L

M

R

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

I

L

S

K

R

Q

L

L

L

F

A

I

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

L
x
I

I
|
I

L

T

Q

T

V

V

S

S

L

I

K

L

V

C

L

P

P

P

R

K

P

P

I

R

A

A

binding flavin-adenine dinucleotide: P75 (≠ I26), G77 (= G28), G78 (= G29), N79 (≠ G30), T80 (= T31), G81 (≠ K32), G85 (= G36), S86 (≠ Q37), L139 (≠ F91), G140 (= G92), A141 (vs. gap), C145 (≠ A95), H146 (≠ T96), G148 (= G98), G149 (= G99), N150 (≠ M100), A152 (= A102), T153 (≠ A103), A155 (≠ L105), E206 (≠ L156), G207 (= G157), I211 (≠ L161), I212 (= I162)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39, 422, 459
binding (2s)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 423
binding zinc ion: 381, 388, 422

Query Sequence

>WP_088755699.1 NCBI__GCF_002213425.1:WP_088755699.1
MQAQQDMEAVLASFRERILSGRALQIRGGGTKDWYGQVPSGDLLDTRPYSGIIDYEPTEL
VITARCGTPLAEIDAALAGHNQMLAWEPPRFGGAATVGGMVAAGLSGPSRAAAGAVRDFV
LGAVLMDAQGEQLHFGGQVMKNVAGYDISRLLAGSLGTLGLILQVSLKVLPRPIASSTRV
FEINQEGALRLLNQSAGQPLPLVASAWVNNVLTIRLSGAQAAVDSAIKKMGGTELSNPED
YWTDLREQTHPFFADPQAPLWRLSVPSVAPPIDLPGRQLIEWGGAQRWLRPEGNVDAVAI
RAAALAVGGHASLHRGGDKAIGVFHPLQPAVESIHRRLKQQFDPQGIFNPGRLYASL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory