SitesBLAST
Comparing WP_088755932.1 NCBI__GCF_002213425.1:WP_088755932.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
44% identity, 99% coverage: 5:471/471 of query aligns to 3:465/465 of 3pm9A
- active site: A149 (= A153), L159 (≠ N163)
- binding flavin-adenine dinucleotide: P69 (≠ T71), Q70 (= Q72), G71 (= G73), G72 (= G74), N73 (= N75), T74 (= T76), G75 (= G77), L76 (≠ M78), G79 (= G81), Q80 (≠ A82), L91 (≠ T95), L133 (= L137), G134 (= G138), A135 (= A139), C139 (= C143), T140 (= T144), G142 (= G146), G143 (= G147), S146 (≠ A150), T147 (= T151), A149 (= A153), G150 (= G154), E200 (= E204), G201 (= G205), I205 (≠ V209), I206 (= I210), E423 (= E429)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
37% identity, 99% coverage: 5:471/471 of query aligns to 10:464/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R333), T337 (≠ S337), K348 (= K348), Y379 (≠ F381), H381 (= H383), H388 (= H390), H423 (= H430)
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (≠ T71), Q76 (= Q72), G77 (= G73), G78 (= G74), N79 (= N75), T80 (= T76), G81 (= G77), M82 (= M78), G85 (= G81), S86 (≠ A82), L139 (= L137), G140 (= G138), A141 (= A139), C145 (= C143), G149 (= G147), N150 (= N148), A152 (= A150), T153 (= T151), G157 (= G155), G207 (= G205), I212 (= I210), E422 (= E429), N459 (= N466)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
37% identity, 99% coverage: 5:471/471 of query aligns to 10:464/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (≠ T71), G77 (= G73), G78 (= G74), N79 (= N75), T80 (= T76), G81 (= G77), G85 (= G81), S86 (≠ A82), L139 (= L137), G140 (= G138), A141 (= A139), C145 (= C143), H146 (≠ T144), G148 (= G146), G149 (= G147), N150 (= N148), A152 (= A150), T153 (= T151), A155 (= A153), E206 (= E204), G207 (= G205), I211 (≠ V209), I212 (= I210), E422 (= E429), N459 (= N466)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R333), T337 (≠ S337), K348 (= K348), Y379 (≠ F381), H381 (= H383), H388 (= H390), H423 (= H430)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
37% identity, 99% coverage: 5:471/471 of query aligns to 10:464/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (≠ T71), G77 (= G73), G78 (= G74), N79 (= N75), T80 (= T76), G81 (= G77), G85 (= G81), S86 (≠ A82), L139 (= L137), G140 (= G138), A141 (= A139), C145 (= C143), H146 (≠ T144), G149 (= G147), N150 (= N148), A152 (= A150), T153 (= T151), A155 (= A153), G157 (= G155), E206 (= E204), G207 (= G205), I211 (≠ V209), I212 (= I210), E422 (= E429), N459 (= N466)
- binding d-malate: M82 (= M78), R333 (= R333), T337 (≠ S337), K348 (= K348), Y379 (≠ F381), H381 (= H383), H388 (= H390), E422 (= E429), H423 (= H430)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
37% identity, 99% coverage: 5:471/471 of query aligns to 10:464/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R333), T337 (≠ S337), K348 (= K348), Y379 (≠ F381), H381 (= H383), H388 (= H390), N390 (= N392), H423 (= H430)
- binding flavin-adenine dinucleotide: W39 (= W35), P75 (≠ T71), G77 (= G73), G78 (= G74), N79 (= N75), T80 (= T76), G81 (= G77), M82 (= M78), G85 (= G81), S86 (≠ A82), L139 (= L137), G140 (= G138), A141 (= A139), C145 (= C143), G149 (= G147), N150 (= N148), A152 (= A150), T153 (= T151), A155 (= A153), G157 (= G155), G207 (= G205), I212 (= I210), E422 (= E429), H423 (= H430)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
37% identity, 99% coverage: 5:471/471 of query aligns to 11:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W35), P76 (≠ T71), G78 (= G73), G79 (= G74), N80 (= N75), T81 (= T76), G82 (= G77), M83 (= M78), G86 (= G81), S87 (≠ A82), L140 (= L137), A142 (= A139), C146 (= C143), H147 (≠ T144), G150 (= G147), N151 (= N148), A153 (= A150), T154 (= T151), G208 (= G205), I212 (≠ V209), I213 (= I210), E423 (= E429), N460 (= N466)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
37% identity, 99% coverage: 5:471/471 of query aligns to 63:517/521 of Q8N465
- S109 (≠ T52) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V70) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G74) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (= I92) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (= M98) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V117) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P134) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A150) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P176) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G178) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ N322) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R333) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ S337) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ L346) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K348) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ E366) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ Q375) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H383) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G385) to V: slight reduction in catalytic activity
- N439 (= N388) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H390) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (= N392) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (= V393) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ S398) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E429) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H430) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G431) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 98% coverage: 9:471/471 of query aligns to 32:496/496 of P39976