SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_089136490.1 NCBI__GCF_002217945.1:WP_089136490.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3zlbA Crystal structure of phosphoglycerate kinase from streptococcus pneumoniae (see paper)
62% identity, 100% coverage: 1:400/400 of query aligns to 1:398/398 of 3zlbA

query
sites
3zlbA

M
|
M

A

A

K

K

L

L

T

T

V

V

S

K

D
|
D

L

V

D

D

L

L

K

K

D

G

K

K

K

K

V

V

L

L

M

V

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

L

K

K

N

D

G

G

V

V

I

I

G

T

N

N

D

D

N

N

R
|
R

I

I

V

T

A

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

V

I

I

I

E

E

H

Q

D

G

G

G

K

R

A

A

I

I

L

L

L

F

S

S

H

H

L

L

G

G

R

R

V

V

K

K

S

E

E

E

D

S

D

D

K

K

P

A

G

G

L

K

S

S

M

L

R

A

P

P

V

V

A

A

E

A

R

D

L

L

S

A

N

A

L

K

L

L

N

G

K

Q

P

D

V

V

T

V

F

F

V

P

P

G

V

V

T

T

E

R

G

G

K

A

Q

E

L

L

E

E

D

A

A

A

I

I

N

N

N

A

M

L

N

E

D

D

G

G

D

Q

V

V

L

L

V

L

M

V

E

E

N

N

T

T

R

R

Y

Y

E

E

D

D

V

V

K

D

N

G

G

-

E

-

Q

-

V

-

K

K

R

K

E

E

S

S

K

K

N

N

D

D

P

P

A

E

L

L

G

G

K

K

Y

Y

W

W

A

A

S

S

L

L

G

G

D

D

-

G

V

I

F

F

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

Q

A

H

H

A

A

S

S

N

N

V

V

G

G

I

I

A

S

S

A

N

N

M

V

P

E

Q

K

T

A

A

V

A

A

G

G

F

F

L

L

M

L

E

E

K

N

E

E

I

I

K

A

F

Y

I

I

G

Q

G

E

A

A

V

V

S

E

H

T

P

P

A

E

H

R

P

P

F

F

V

V

A

A

I

I

L

L

G

G

G
|
G

A
x
S

K
|
K

V

V

S

S

D

D

K

K

I

I

G

G

V

V

I

I

D

E

N

N

L

L

L

L

D

E

K

K

A

A

D

D

K

K

V

V

I

L

I

I

G

G

G
|
G

G
|
G

M

M

T

T

Y

Y

T

T

F

F

Y

Y

A

K

A

A

K

Q

G

G

M

I

K

E

I

I

G

G

N

N

S

S

L

L

V

V

E

E

K

E

D

D

K

K

I

L

D

D

L

V

A

A

K

K

E

A

I

L

M

L

D

E

K

K

A

A

G

N

D

G

K

K

L

L

V

I

L

L

P

P

V

V

D

D

S

S

V

K

V

E

A

A

E

N

K

A

F

F

D

A

N

G

D

Y

A

T

A

E

H

V

K

R

T

D

V

T

E

E

G

G

D

E

-

A

I

V

P

S

D

E

G

G

Y

F

M

L

A

G

L

L

D

D

I

I

G

G

P

P

K

K

S

S

I

I

A

A

Q

K

F

F

E

D

D

E

V

A

L

L

K

T

S

G

A

A

K

K

L

T

V

V

V

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

N

S

P

N

D

Y

F

A

Q

E

A

G

G

T

T

L

I

E

G

I

V

G

M

K

D

Y

A

L

I

G

V

T

K

L

Q

T

P

D

G

A

V

T

K

T

S

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

T

A

A

A

A

A

V

A

Q

I

Q

N

L

L

G

G

V

R

G

A

D

D

K

K

L

F

T

S

H

W

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

M

E

E

Y

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

I

L

A

A

A

A

I

L

S

T

E

E

K
|
K

active site: R36 (= R36), K204 (= K207), G355 (= G357), G378 (= G380)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G205), S203 (≠ A206), G226 (= G229), G227 (= G230), N320 (= N322), P322 (= P324), G324 (= G326), V325 (= V327), E327 (= E329), G354 (= G356), G355 (= G357), D356 (= D358), S357 (= S359)
binding magnesium ion: M1 (= M1), D8 (= D8), K398 (= K400)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
56% identity, 100% coverage: 1:400/400 of query aligns to 1:397/654 of P36204

query
sites
P36204

M

M

A

E

K

K

L

M

T

T

V

I

S

R

D

D

L

V

D

D

L

L

K

K

D

G

K

K

K

R

V

V

L

I

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

V

K

K

N

D

G

G

V

V

I

V

G

Q

N

D

D

D

N

T

R
|
R

I

I

V

R

A

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

V

A

I

L

E

E

H

Q

D

G

G

A

K

K

A

V

I

I

L

L

L

L

S

S

H

H

L

L

G

G

R

R

V

P

K

K

S

G

E

E

D

P

D

S

K

-

P

P

G

E

L

F

S

S

M

L

R

A

P

P

V

V

A

A

E

K

R

R

L

L

S

S

N

E

L

L

L

L

N

G

K

K

P

E

V

V

T

K

F

F

V

V

P

P

V

A

T

V

E

V

G

G

K

D

Q

E

L

V

E

K

D

K

A

A

I

V

N

E

N

E

M

L

N

K

D

E

G

G

D

E

V

V

L

L

V

L

M

L

E

E

N

N

T

T

R
|
R

Y

F

E

H

D

P

V

-

K

-

N

-

G

-

E

-

Q

-

V

-

K

-

R

G

E

E

S

T

K

K

N

N

D

D

P

P

A

E

L

L

G

A

K

K

Y

F

W

W

A

A

S

S

L

L

G

A

D

D

V

I

F

H

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

Q

A

H

H

A

A

S

S

N

N

V

V

G

G

I

I

A

A

S

Q

N

F

M

I

P

P

Q

-

T

S

A

V

A

A

G

G

F

F

L

L

M

M

E

E

K

K

E

E

I

I

K

K

F

F

I

L

G

S

G

K

A

V

V

T

S

Y

H

N

P

P

A

E

H

K

P

P

F

Y

V

V

A

V

I

V

L

L

G

G

G

G

A

A

K

K

V

V

S

S

D

D

K

K

I

I

G

G

V

V

I

I

D

T

N

N

L

L

L

M

D

E

K

K

A

A

D

D

K

R

V

I

I

L

I

I

G

G

G

G

G

A

M

M

T

M

Y

F

T

T

F

F

Y

L

A

K

A

A

K

L

G

G

M

K

K

E

I

V

G

G

N

S

S

S

L

R

V

V

E

E

K

E

D

D

K

K

I

I

D

D

L

L

A

A

K

K

E

E

I

L

M

L

D

E

K

K

A

A

G

K

D

E

K

K

-

G

-

V

-

E

L

I

V

V

L

L

P

P

V

V

D

D

S

A

V

V

V

I

A

A

E

Q

K

K

F

I

D

E

N

P

D

G

A

V

A

E

H

K

K

K

T

V

V

V

E

R

G

I

D

D

-

D

-

G

I

I

P

P

D

E

G

G

Y

W

M

M

A

G

L

L

D

D

I

I

G

G

P

P

K

E

S

T

I

I

A

E

Q

L

F

F

E

K

D

Q

V

K

L

L

K

S

S

D

A

A

K

K

L

T

V

V

V

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

F

F

E

E

M

I

S

D

N

D

Y

F

A

A

E

E

G

G

T

T

L

K

E

Q

I

V

G

A

K

L

Y

A

L

I

G

A

T

A

L

L

T

T

D

E

-

K

-

G

A

A

T

I

T

T

I

V

V

V

G

G

G

G

G

G

D

D

S

S

T

A

A

A

A

A

V

V

Q

N

Q

K

L

F

G

G

V

L

G

E

D

D

K

K

L

F

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

Y

F

L

L

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

I

A

A

A

S

I

I

S

A

E

D

K

K

R36 (= R36) binding substrate
R118 (= R119) binding substrate
R151 (= R160) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
56% identity, 100% coverage: 3:400/400 of query aligns to 2:396/398 of 1vpeA

query
sites
1vpeA

K

K

L

M

T

T

V

I

S

R

D

D

L

V

D

D

L

L

K

K

D

G

K

K

K

R

V

V

L

I

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

V

K

K

N

D

G

G

V

V

I

V

G

Q

N

D

D

D

N

T

R
|
R

I

I

V

R

A

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

V

A

I

L

E

E

H

Q

D

G

G

A

K

K

A

V

I

I

L

L

L

L

S

S

H

H

L

L

G

G

R

R

V

P

K

K

S

G

E

E

D

P

D

S

K

-

P

P

G

E

L

F

S

S

M

L

R

A

P

P

V

V

A

A

E

K

R

R

L

L

S

S

N

E

L

L

L

L

N

G

K

K

P

E

V

V

T

K

F

F

V

V

P

P

V

A

T

V

E

V

G

G

K

D

Q

E

L

V

E

K

D

K

A

A

I

V

N

E

N

E

M

L

N

K

D

E

G

G

D

E

V

V

L

L

V

L

M

L

E

E

N

N

T

T

R

R

Y

F

E

H

D

P

V

-

K

-

N

-

G

-

E

-

Q

-

V

-

K

-

R

G

E

E

S

T

K

K

N

N

D

D

P

P

A

E

L

L

G

A

K

K

Y

F

W

W

A

A

S

S

L

L

G

A

D

D

V

I

F

H

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

Q

A

H

H

A

A

S

S

N

N

V

V

G

G

I

I

A

A

S

Q

N

F

M

I

P

P

Q

-

T

S

A

V

A

A

G

G

F

F

L

L

M

M

E

E

K

K

E

E

I

I

K

K

F

F

I

L

G

S

G

K

A

V

V

T

S

Y

H

N

P

P

A

E

H

K

P

P

F

Y

V

V

A

V

I

V

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

G

G

V

V

I

I

D

T

N

N

L

L

L

M

D

E

K

K

A

A

D

D

K

R

V

I

I

L

I

I

G

G

G
|
G

G
x
A

M

M

T

M

Y

F

T

T

F

F

Y

L

A

K

A

A

K

L

G

G

M

K

K

E

I

V

G

G

N

S

S

S

L

R

V

V

E

E

K

E

D

D

K

K

I

I

D

D

L

L

A

A

K

K

E

E

I

L

M

V

D

E

K

K

A

A

G

K

D

E

K

K

-

G

-

V

-

E

L

I

V

V

L

L

P

P

V

V

D

D

S

A

V

V

V

I

A

A

E

Q

K

K

F

I

D

E

N

P

D

G

A

V

A

E

H

K

K

K

T

V

V

V

E

R

G

I

D

D

-

D

-

G

I

I

P

P

D

E

G

G

Y

W

M

M

A

G

L

L

D

D

I

I

G

G

P

P

K

E

S

T

I

I

A

E

Q

L

F

F

E

K

D

Q

V

K

L

L

K

S

S

D

A

A

K

K

L

T

V

V

V

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

I

S

D

N

D

Y

F

A

A

E

E

G

G

T

T

L

K

E

Q

I

V

G

A

K

L

Y

A

L

I

G

A

T

A

L

L

T

T

D

E

-

K

-

G

A

A

T

I

T

T

I

V

V

V

G

G

G
|
G

G
|
G

D
|
D

S
|
S

T

A

A

A

A

A

V

V

Q

N

Q

K

L

F

G

G

V

L

G

E

D

D

K

K

L

F

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

F

L

L

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

I

A

A

A

S

I

M

S

R

E

I

K

K

active site: R35 (= R36), K196 (= K207), G353 (= G357), G376 (= G380)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G205), A195 (= A206), K196 (= K207), K200 (= K211), G218 (= G229), A219 (≠ G230), N316 (= N322), P318 (= P324), G320 (= G326), V321 (= V327), E323 (= E329), G352 (= G356), G353 (= G357), D354 (= D358), S355 (= S359)

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
53% identity, 100% coverage: 1:400/400 of query aligns to 1:394/394 of 1phpA

query
sites
1phpA

M

M

A

N

K

K

L

K

T

T

V

I

S

R

D

D

L

V

D

D

L

V

K

R

D

G

K

K

K

R

V

V

L

F

M

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

M

K

E

N

Q

G

G

V

A

I

I

G

T

N

D

D

D

N

T

R
|
R

I

I

V

R

A

A

A

A

L

L

P

P

T

T

I

I

K

R

Y

Y

V

L

I

I

E

E

H

H

D

G

G

A

K

K

A

V

I

I

L

L

L

A

S

S

H

H

L

L

G

G

R

R

V

P

K

K

S

G

E

K

D

V

D

V

K

E

P

E

G

-

L

L

S

R

M

L

R

D

P

A

V

V

A

A

E

K

R

R

L

L

S

G

N

E

L

L

L

L

N

E

K

R

P

P

V

V

T

A

F

K

V

T

P

N

V

E

T

A

E

V

G

G

K

D

Q

E

L

V

E

K

D

A

A

A

I

V

N

D

N

R

M

L

N

N

D

E

G

G

D

D

V

V

L

L

V

L

M

L

E

E

N

N

T

V

R

R

Y

F

E

-

D

-

V

-

K

-

N

-

G

-

E

-

Q

-

V

Y

K

P

R

G

E

E

S

E

K

K

N

N

D

D

P

P

A

E

L

L

G

A

K

K

Y

A

W

F

A

A

S

E

L

L

G

A

D

D

V

L

F

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

Q

A

H

H

A

A

S

S

N

T

V

E

G

G

I

I

A

A

S

H

N

Y

M

L

P

P

Q

-

T

A

A

V

A

A

G

G

F

F

L

L

M

M

E

E

K

K

E

E

I

L

K

E

F

V

I

L

G

G

G

K

A

A

V

L

S

S

H

N

P

P

A

D

H

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

G
|
G

A

A

K
|
K

V

V

S

K

D

D

K
|
K

I

I

G

G

V

V

I

I

D

D

N

N

L

L

L

L

D

E

K

K

A

V

D

D

K

N

V

L

I

I

I

I

G

G

G
|
G

G
|
G

M

L

T

A

Y

Y

T

T

F

F

Y

V

A

K

A

A

K

L

G

G

M

H

K

D

I

V

G

G

N

K

S

S

L
|
L

V

L

E

E

K

E

D

D

K

K

I

I

D

E

L

L

A

A

K

K

E

S

I

F

M

M

D

E

K

K

A

A

-

K

-

E

-

K

G

G

D

V

K

R

L

F

V

Y

L

M

P

P

V

V

D

D

S

V

V

V

V

V

A

A

E

D

K

R

F

F

D

A

N

N

D

D

A

A

A

N

H

T

K

K

T

V

V

V

E

P

G

I

D

D

-

A

I

I

P

P

D

A

G

D

Y

W

M

S

A

A

L

L

D

D

I

I

G

G

P

P

K

K

S

T

I

R

A

E

Q

L

F

Y

E

R

D

D

V

V

L

I

K

R

S

E

A

S

K

K

L

L

V

V

V

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

S

D

N

A

Y

F

A

A

E

H

G

G

T

T

L

K

E

A

I

I

G

A

K

E

Y

A

L

L

G

A

T

E

L

A

T

L

D

D

A

T

T

Y

T

S

I

V

V

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

T

A

A

A

A

A

V

V

Q

E

Q

K

L

F

G

G

V

L

G

A

D

D

K

K

L

M

T

D

H

H

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

F

L

M

E

E

G

G

K

K

T

Q

L

L

P

P

G

G

I

V

A

V

A

A

I

L

S

E

E

D

K

K

active site: R36 (= R36), K197 (= K207), G351 (= G357), G374 (= G380)
binding adenosine-5'-diphosphate: G195 (= G205), K201 (= K211), G219 (= G229), G220 (= G230), L237 (= L247), N316 (= N322), P318 (= P324), G320 (= G326), V321 (= V327), E323 (= E329), G350 (= G356), D352 (= D358), S353 (= S359)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
53% identity, 100% coverage: 1:400/400 of query aligns to 1:394/394 of P18912

query
sites
P18912

M

M

A

N

K

K

L

K

T

T

V

I

S

R

D

D

L

V

D

D

L

V

K

R

D

G

K

K

K

R

V

V

L

F

M

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

M

K

E

N

Q

G

G

V

A

I

I

G

T

N

D

D

D

N

T

R

R

I

I

V

R

A

A

A

A

L

L

P

P

T

T

I

I

K

R

Y

Y

V

L

I

I

E

E

H

H

D

G

G

A

K

K

A

V

I

I

L

L

L

A

S

S

H

H

L

L

G

G

R

R

V

P

K

K

S

G

E

K

D

V

D

V

K

E

P

E

G

-

L

L

S

R

M

L

R

D

P

A

V

V

A

A

E

K

R

R

L

L

S

G

N

E

L

L

L

L

N

E

K

R

P

P

V

V

T

A

F

K

V

T

P

N

V

E

T

A

E

V

G

G

K

D

Q

E

L

V

E

K

D

A

A

A

I

V

N

D

N

R

M

L

N

N

D

E

G

G

D

D

V

V

L

L

V

L

M

L

E

E

N

N

T

V

R

R

Y

F

E

-

D

-

V

-

K

-

N

-

G

-

E

-

Q

-

V

Y

K

P

R

G

E

E

S

E

K

K

N

N

D

D

P

P

A

E

L

L

G

A

K

K

Y

A

W

F

A

A

S

E

L

L

G

A

D

D

V

L

F

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

Q

A

H

H

A

A

S

S

N

T

V

E

G

G

I

I

A

A

S

H

N

Y

M

L

P

P

Q

-

T

A

A

V

A

A

G

G

F

F

L

L

M

M

E

E

K

K

E

E

I

L

K

E

F

V

I

L

G

G

G

K

A

A

V

L

S

S

H

N

P

P

A

D

H

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

G

G

A

A

K

K

V

V

S

K

D

D

K
|
K

I

I

G

G

V

V

I

I

D

D

N

N

L

L

L

L

D

E

K

K

A

V

D

D

K

N

V

L

I

I

I

I

G

G

G

G

G

G

M

L

T

A

Y

Y

T

T

F

F

Y

V

A

K

A

A

K

L

G

G

M

H

K

D

I

V

G

G

N

K

S

S

L

L

V

L

E

E

K

E

D

D

K

K

I

I

D

E

L

L

A

A

K

K

E

S

I

F

M

M

D

E

K

K

A

A

-

K

-

E

-

K

G

G

D

V

K

R

L

F

V

Y

L

M

P

P

V

V

D

D

S

V

V

V

V

V

A

A

E

D

K

R

F

F

D

A

N

N

D

D

A

A

A

N

H

T

K

K

T

V

V

V

E

P

G

I

D

D

-

A

I

I

P

P

D

A

G

D

Y

W

M

S

A

A

L

L

D

D

I

I

G

G

P

P

K

K

S

T

I

R

A

E

Q

L

F

Y

E

R

D

D

V

V

L

I

K

R

S

E

A

S

K

K

L

L

V

V

V

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

M

M

S

D

N

A

Y

F

A

A

E

H

G

G

T

T

L

K

E

A

I

I

G

A

K

E

Y

A

L

L

G

A

T

E

L

A

T

L

D

D

A

T

T

Y

T

S

I

V

V

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

T

A

A

A

A

A

V

V

Q

E

Q

K

L

F

G

G

V

L

G

A

D

D

K

K

L

M

T

D

H

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

Y

F

L

M

E

E

G

G

K

K

T

Q

L

L

P

P

G

G

I

V

A

V

A

A

I

L

S

E

E

D

K

K

K201 (= K211) binding ATP
N316 (= N322) binding ATP
E323 (= E329) binding ATP
GGDS 350:353 (= GGDS 356:359) binding ATP

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
52% identity, 100% coverage: 1:400/400 of query aligns to 1:394/394 of P40924

query
sites
P40924

M

M

A

N

K

K

L

K

T

T

V

L

S

K

D

D

L

I

D

D

L

V

K

K

D

G

K

K

K

V

V

V

L

F

M

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

M

K

K

N

D

G

G

V

E

I

V

G

T

N

D

D

D

N

T

R

R

I

I

V

R

A

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

H

V

L

I

A

E

D

H

Q

D

G

G

A

K

K

A

V

I

L

L

L

L

A

S

S

H

H

L

L

G

G

R

R

V

P

K

K

S

G

E

E

D

V

D

V

K

E

P

E

G

-

L

L

S

R

M

L

R

T

P

P

V

V

A

A

E

A

R

R

L

L

S

G

N

E

L

L

L

L

N

G

K

K

P

E

V

V

T

K

F

K

V

A

P

D

V

E

T

A

E

Y

G

G

K

D

Q

A

L

V

E

K

D

A

A

Q

I

I

N

S

N

E

M

M

N

K

D

D

G

G

D

D

V

V

L

L

V

V

M

L

E

E

N

N

T

V

R

R

Y

F

E

-

D

-

V

-

K

-

N

-

G

-

E

-

Q

-

V

Y

K

P

R

G

E

E

S

E

K

K

N

N

D

D

P

P

A

E

L

L

G

A

K

K

Y

A

W

F

A

A

S

E

L

L

G

A

D

D

V

V

F

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

Q

A

H

H

A

A

S

S

N

T

V

A

G

G

I

I

A

A

S

E

N

H

M

L

P

P

Q

A

T

V

A

A

A

-

G

G

F

F

L

L

M

M

E

E

K

K

E

E

I

L

K

D

F

V

I

L

G

G

G

K

A

A

V

V

S
|
S

H

N

P

P

A

D

H

R

P

P

F

F

V

T

A

A

I

I

L

I

G

G

G

G

A

A

K

K

V

V

S

K

D

D

K

K

I

I

G

G

V

V

I

I

D

E

N

S

L

L

L

L

D

D

K

K

A

V

D

D

K

N

V

L

I

I

I

I

G

G

G

G

G

G

M

L

T

A

Y

Y

T

T

F

F

Y

V

A

K

A

A

K

L

G

G

M

Y

K

E

I

V

G

G

N

K

S

S

L

L

V

L

E

E

K

E

D

D

K

K

I

I

D

E

L

L

A

A

K

K

E

S

I

F

M

M

D

D

K

R

A

A

G

K

D

E

K

K

-

G

-

V

-

N

L

F

V

Y

L

M

P

P

V

E

D

D

S

V

V

L

V

V

A

A

E

D

K

D

F

F

D

S

N

N

D

D

A

A

A

N

H

V

K

K

T

I

V

V

E

P

-

I

G

S

D

E

I

I

P

P

D

S

G

D

Y

L

M

E

A

A

L

I

D

D

I

I

G

G

P

T

K

K

S
x
T

I

R

A

E

Q

T

F

Y

E

A

D

D

V

V

L

I

K

K

S

N

A

S

K

K

L

L

V

V

V

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

F

F

E

E

M

I

S

D

N

L

Y

F

A

A

E

Q

G

G

T

T

L

K

E

A

I

V

G

A

K

E

Y

A

L

L

G

A

T

E

L

A

T

K

D

D

A

T

T

Y

T

S

I

V

V

I

G

G

G

G

G

G

D

D

S

S

T

A

A

A

A

A

V

V

Q

E

Q

K

L

F

G

G

V

L

G

A

D

D

K

K

L

M

T

S

H

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

Y

F

L

M

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

V

A

A

A

A

I

L

S

N

E

D

K

K

S183 (= S193) modified: Phosphoserine
T299 (≠ S305) modified: Phosphothreonine

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
45% identity, 100% coverage: 3:400/400 of query aligns to 2:415/415 of 16pkA

query
sites
16pkA

K

K

L

K

T

S

V

I

S

N

D

E

L

C

D

D

L

L

K

K

D

G

K

K

K

K

V

V

L

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

V

K

K

N

N

G

G

V

K

I

I

G

T

N

N

D

D

N

Y

R
|
R

I

I

V

R

A

S

A

A

L

L

P

P

T

T

I

L

K

K

Y

K

V

V

I

L

E

T

H

E

D

G

G

G

K

S

A

C

I

V

L

L

L

M

S

S

H

H

L

L

G

G

R

R

-

P

-

K

-

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

V

I

K

R

S

S

E

T

D

G

D

G

K

V

P

P

G

G

L

F

-

Q

-

Q

-

K

-

A

S

T

M

L

R

K

P

P

V

V

A

A

E

K

R

R

L

L

S

S

N

E

L

L

L

L

N

L

K

R

P

P

V

V

T

T

F

F

V

A

P

P

V

-

T

-

E

D

G

C

K

L

Q

N

L

A

E

A

D

D

A

V

I

V

N

S

N

K

M

M

N

S

D

P

G

G

D

D

V

V

L

V

V

L

M

L

E

E

N

N

T

V

R

R

Y

F

E

Y

D

K

V

-

K

-

N

-

G

-

E

E

Q

E

V

G

K

S

R

K

E

K

S

A

K

K

N

D

D

R

P

E

A

A

L

M

G

A

K

K

Y

I

W

L

A

A

S

S

L

Y

G

G

D

D

V

V

F

Y

I

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

Q

D

H

S

A

A

S

T

N

M

V

T

G

G

I

I

A

P

S

K

N

I

M

L

P

G

Q

N

T

G

A

A

A

A

G

G

F

Y

L

L

M

M

E

E

K

K

E

E

I

I

K

S

F

Y

I

F

G

A

G

K

A

V

V

L

S

G

H

N

P

P

A

P

H

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

G

Q

V

L

I

L

D

D

N

N

L

M

L

L

D

Q

K

R

A

I

D

D

K

Y

V

L

I

L

I

I

G

G

G

G

G
x
A

M

M

T

A

Y
|
Y

T

T

F

F

Y

L

A

K

A

A

K

Q

G

G

M

Y

K

S

I

I

G

G

N

K

S

S

L

K

V

C

E

E

K

E

D

S

K

K

I

L

D

E

L

F

A

A

K

R

E

S

I

L

M

L

D

K

K

K

A

A

G

E

D

D

K

R

-

K

-

V

-

Q

L

V

V

I

L

L

P

P

V

I

D

D

S

H

V

V

V

C

A

H

E

T

K

E

F

F

D

K

N

A

-

V

D

D

A

S

A

P

H

L

K

I

T

T

V

E

E

D

G

Q

D

N

I

I

P

P

D

E

G

G

Y

H

M

M

A

A

L
|
L

D

D

I

I

G

G

P

P

K

K

S

T

I

I

A

E

Q

K

F

Y

E

V

D

Q

V

T

L

I

K

G

S

K

A

C

K

K

L

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

S

V

N

P

Y

Y

A

S

E

K

G

G

T

T

L

F

E

A

I

I

G

A

K

K

Y

A

L

M

G

G

T

R

L

G

T

T

D

H

A

E

-

H

-

G

-

L

T

M

T

S

I

I

V

I

G

G

G

G

G
|
G

D

D

S

S

T

A

A

S

A

A

V

A

Q

E

Q

L

L

S

G

G

V

E

G

A

D

K

K

R

L

M

T

S

H

H

I

V

S

S

T

T

G
|
G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

G

G

I

V

A

T

A

V

I

L

S

D

E

D

K

K

active site: R35 (= R36), K215 (= K207), G372 (= G357), G395 (= G380)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G205), A214 (= A206), K219 (= K211), A238 (≠ G230), Y241 (= Y233), L311 (= L299), P336 (= P324), G338 (= G326), V339 (= V327), E341 (= E329), G393 (= G378), G394 (= G379), G395 (= G380)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
45% identity, 100% coverage: 3:400/400 of query aligns to 2:415/415 of 13pkA

query
sites
13pkA

K

K

L

K

T

S

V

I

S

N

D

E

L

C

D

D

L

L

K

K

D

G

K

K

K

K

V

V

L

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

V

K

K

N

N

G

G

V

K

I

I

G

T

N

N

D

D

N

Y

R
|
R

I

I

V

R

A

S

A

A

L

L

P

P

T

T

I

L

K

K

Y

K

V

V

I

L

E

T

H

E

D

G

G

G

K

S

A

C

I

V

L

L

L

M

S

S

H

H

L

L

G

G

R

R

-

P

-

K

-

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

V

I

K

R

S

S

E

T

D

G

D

G

K

V

P

P

G

G

L

F

-

Q

-

Q

-

K

-

A

S

T

M

L

R

K

P

P

V

V

A

A

E

K

R

R

L

L

S

S

N

E

L

L

L

L

N

L

K

R

P

P

V

V

T

T

F

F

V

A

P

P

V

-

T

-

E

D

G

C

K

L

Q

N

L

A

E

A

D

D

A

V

I

V

N

S

N

K

M

M

N

S

D

P

G

G

D

D

V

V

L

V

V

L

M

L

E

E

N

N

T

V

R

R

Y

F

E

Y

D

K

V

-

K

-

N

-

G

-

E

E

Q

E

V

G

K

S

R

K

E

K

S

A

K

K

N

D

D

R

P

E

A

A

L

M

G

A

K

K

Y

I

W

L

A

A

S

S

L

Y

G

G

D

D

V

V

F

Y

I

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

Q

D

H

S

A

A

S

T

N

M

V

T

G

G

I

I

A

P

S

K

N

I

M

L

P

G

Q

N

T

G

A

A

A

A

G

G

F

Y

L

L

M

M

E

E

K

K

E

E

I

I

K

S

F

Y

I

F

G

A

G

K

A

V

V

L

S

G

H

N

P

P

A

P

H

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

G

Q

V

L

I

L

D

D

N

N

L

M

L

L

D

Q

K

R

A

I

D

D

K

Y

V

L

I

L

I

I

G

G

G

G

G

A

M

M

T

A

Y

Y

T

T

F

F

Y

L

A

K

A

A

K

Q

G

G

M

Y

K

S

I

I

G

G

N

K

S

S

L

K

V

C

E

E

K

E

D

S

K

K

I

L

D

E

L

F

A

A

K

R

E

S

I

L

M

L

D

K

K

K

A

A

G

E

D

D

K

R

-

K

-

V

-

Q

L

V

V

I

L

L

P

P

V

I

D

D

S

H

V

V

V

C

A

H

E

T

K

E

F

F

D

K

N

A

-

V

D

D

A

S

A

P

H

L

K

I

T

T

V

E

E

D

G

Q

D

N

I

I

P

P

D

E

G

G

Y

H

M

M

A

A

L
|
L

D

D

I

I

G

G

P

P

K

K

S

T

I

I

A

E

Q

K

F

Y

E

V

D

Q

V

T

L

I

K

G

S

K

A

C

K

K

L

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

S

V

N

P

Y

Y

A

S

E

K

G

G

T

T

L

F

E

A

I

I

G

A

K

K

Y

A

L

M

G

G

T

R

L

G

T

T

D

H

A

E

-

H

-

G

-

L

T

M

T

S

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

T

A

A

S

A

A

V

A

Q

E

Q

L

L

S

G

G

V

E

G

A

D

K

K

R

L

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

G

G

I

V

A

T

A

V

I

L

S

D

E

D

K

K

active site: R35 (= R36), K215 (= K207), G372 (= G357), G395 (= G380)
binding adenosine-5'-diphosphate: G213 (= G205), A214 (= A206), K219 (= K211), L311 (= L299), P336 (= P324), G338 (= G326), V339 (= V327), E341 (= E329), G371 (= G356), D373 (= D358), S374 (= S359)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
45% identity, 100% coverage: 3:400/400 of query aligns to 6:419/440 of P07378

query
sites
P07378

K

K

L

K

T

S

V

I

S

N

D

E

L

C

D

D

L

L

K

K

D

G

K

K

K

K

V

V

L

L

M

I

R

R

V

V

D
|
D

F
|
F

N
|
N

V

V

P

P

I

V

K

K

N

N

G

G

V

K

I

I

G

T

N

N

D

D

N

Y

R
|
R

I

I

V

R

A

S

A

A

L

L

P

P

T

T

I

L

K

K

Y

K

V

V

I

L

E

T

H

E

D

G

G

G

K

S

A

C

I

V

L

L

L

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

-

P

-

K

-

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

V

I

K

R

S

S

E

T

D

G

D

G

K

V

P

P

G

G

L

F

-

Q

-

Q

-

K

-

A

S

T

M

L

R

K

P

P

V

V

A

A

E

K

R

A

L

L

S

S

N

E

L

L

L

L

N

L

K

R

P

P

V

V

T

T

F

F

V

A

P

P

V

-

T

-

E

D

G

C

K

L

Q

N

L

A

E

A

D

D

A

V

I

V

N

S

N

K

M

M

N

S

D

P

G

G

D

D

V

V

L

V

V

L

M

L

E

E

N

N

T

V

R
|
R

Y

F

E

Y

D

K

V

-

K

-

N

-

G

-

E

E

Q

E

V

G

K

S

R

K

E

K

S

A

K

K

N

D

D

R

P

E

A

A

L

M

G

A

K

K

Y

I

W

L

A

A

S

S

L

Y

G

G

D

D

V

V

F

Y

I

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

Q

D

H

S

A

A

S

T

N

M

V

T

G

G

I

I

A

P

S

K

N

I

M

L

P

G

Q

N

T

G

A

A

A

A

G

G

F

Y

L

L

M

M

E

E

K

K

E

E

I

I

K

S

F

Y

I

F

G

A

G

K

A

V

V

L

S

G

H

N

P

P

A

P

H

R

P

P

F

L

V

V

A

A

I

I

L

V

G

G

G

G

A

A

K

K

V

V

S

S

D

D

K
|
K

I

I

G

Q

V

L

I

L

D

D

N

N

L

M

L

L

D

Q

K

R

A

I

D

D

K

Y

V

L

I

L

I

I

G

G

G

G

G

A

M

M

T

A

Y

Y

T

T

F

F

Y

L

A

K

A

A

K

Q

G

G

M

Y

K

S

I

I

G

G

N

K

S

S

L

K

V

C

E

E

K

E

D

S

K

K

I

L

D

E

L

F

A

A

K

R

E

S

I

L

M

L

D

K

K

K

A

A

G

E

D

D

K

R

-

K

-

V

-

Q

L

V

V

I

L

L

P

P

V

I

D

D

S

H

V

V

V

C

A

H

E

T

K

E

F

F

D

K

N

A

-

V

D

D

A

S

A

P

H

L

K

I

T

T

V

E

E

D

G

Q

D

N

I

I

P

P

D

E

G

G

Y

H

M

M

A

A

L

L

D

D

I

I

G

G

P

P

K

K

S

T

I

I

A

E

Q

K

F

Y

E

V

D

Q

V

T

L

I

K

G

S

K

A

C

K

K

L

S

V

A

V

I

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

M

M

S

V

N

P

Y

Y

A

S

E

K

G

G

T

T

L

F

E

A

I

I

G

A

K

K

Y

A

L

M

G

G

T

R

L

G

T

T

D

H

A

E

-

H

-

G

-

L

T

M

T

S

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

T

A

A

S

A

A

V

A

Q

E

Q

L

L

S

G

G

V

E

G

A

D

K

K

R

L

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

G

G

I

V

A

T

A

V

I

L

S

D

E

E

K

K

DFN 24:26 (= DFN 21:23) binding substrate
R39 (= R36) binding substrate
HLGR 62:65 (= HLGR 59:62) binding substrate
R135 (= R119) binding substrate
R172 (= R160) binding substrate
K223 (= K211) binding ATP
N338 (= N322) binding ATP
E345 (= E329) binding ATP
GGDS 375:378 (= GGDS 356:359) binding ATP

P09041 Phosphoglycerate kinase 2; Phosphoglycerate kinase, testis specific; EC 2.7.2.3 from Mus musculus (Mouse) (see paper)
47% identity, 99% coverage: 2:398/400 of query aligns to 5:415/417 of P09041

query
sites
P09041

A

A

K

K

L

L

T

T

V

L

S

D

D

K

L

V

D

D

L

L

K

K

D

G

K

K

K

R

V

V

L

I

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

M

K

K

N

N

G

N

V

Q

I

I

G

T

N

N

D

N

N

Q

R

R

I

I

V

K

A

A

A

A

L

I

P

P

T

S

I

I

K

K

Y

H

V

C

I

L

E

D

H

N

D

G

G

A

K

K

A

S

I

V

-

V

L

L

L

M

S

S

H

H

L

L

G

G

R

R

V

P

K

D

S

G

E

I

D

P

D

M

K

P

P

D

G

K

L

Y

S

S

M

L

R

E

P

P

V

V

A

A

E

D

R

E

L

L

S

K

N

S

L

L

L

L

N

N

K

K

P

D

V

V

T

I

F

F

V

L

P

K

V

D

T

C

E

V

G

G

K

P

Q

E

L

V

E

E

D

Q

A

A

I

C

N

A

N

N

M

P

N

D

D

N

G

G

D

S

V

I

L

I

V

L

M

L

E

E

N

N

T

L

R

R

Y

F

-

H

-

V

-

E

E

E

D

E

V

G

K

K

N

G

G

K

E

D

Q

S

V

S

K

G

R

K

E

K

S

I

K

S

N

A

D

D

P

P

A

A

-

K

L

V

G

E

K

A

Y

F

W

Q

A

A

S

S

L

L

-

S

-

K

-

L

G

G

D

D

V

V

F

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

Q

A

H

H

A

S

S

S

N

T

V

V

G

G

I

V

A

-

S

-

N

N

M

L

P

P

Q

Q

T

K

A

A

A

S

G

G

F

F

L

L

M

M

E

K

K

K

E

E

I

L

K

D

F

Y

I

F

G

S

G

K

A

A

V

L

S

E

H

K

P

P

A

E

H

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G

G

A

A

K

K

V

V

S

K

D

D

K
|
K

I

I

G

Q

V

L

I

I

D

K

N

N

L

M

L

L

D

D

K

K

A

V

D

N

K

F

V

M

I

I

I

I

G

G

G

G

G

G

M

M

T

A

Y

Y

T

T

F

F

Y

L

A

K

A

E

-

L

K

K

G

N

M

M

K

Q

I

I

G

G

N

A

S

S

L

L

V

F

E

D

K

E

D

E

K

G

I

A

D

T

L

I

A

V

K

K

E

E

I

I

M

M

D

E

K

K

A

A

-

E

-

K

-

N

G

G

D

V

K

K

L

I

V

V

L

F

P

P

V

V

D

D

S

F

V

V

V

T

A

G

E

D

K

K

F

F

D

D

N

E

D

N

A

A

-

K

-

V

A

G

H

Q

K

A

T

T

V

I

E

E

G

S

D

G

I

I

P

P

D

S

G

G

Y

W

M

M

A
x
G

L

L

D

D

I

C

G

G

P

P

K

E

S

S

I

I

A

K

Q

I

F

N

E

A

D

Q

V

I

L

V

K

A

S

Q

A

A

K

K

L

L

V

I

V

V

W

W

N

N

G

G

P

P

M

I

G

G

V

V

F

F

E
|
E

M

W

S

D

N

A

Y

F

A

A

E

K

G

G

T

T

L

K

E

A

I

L

G

M

K

D

Y

E

L

V

-

V

-

K

G

A

T

T

L

S

T

N

D

G

A

C

T

V

T

T

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

T

A

A

T

A

C

V

C

Q

A

Q

K

L

W

G

G

V

T

G

E

D

D

K

K

L

V

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

I

L

L

P

P

G

G

I

V

A

E

A

A

I

L

S

S

K220 (= K211) binding ATP
G313 (≠ A298) binding ATP
E344 (= E329) binding ATP
GGDT 373:376 (≠ GGDS 356:359) binding ATP

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
48% identity, 100% coverage: 2:400/400 of query aligns to 5:415/416 of P00560

query
sites
P00560

A

S

K

K

L

L

T

S

V

V

S

Q

D

D

L

L

D

D

L

L

K

K

D

D

K

K

K

R

V

V

L

F

M

I

R
|
R

V

V

D

D

F

F

N

N

V

V

P

P

I

L

K

D

N

G

G

K

V

K

I

I

G

T

N

S

D

N

N

Q

R
|
R

I

I

V

V

A

A

A

A

L

L

P

P

T

T

I

I

K

K

Y

Y

V

V

I

L

E

E

H

H

D

H

G

P

K

R

A

Y

I

V

L

V

L

L

-

A

S

S

H

H

L

L

G

G

R

R

V

P

K

N

S

G

E

E

D

R

D

N

K

E

P

K

G

-

L

Y

S

S

M

L

R

A

P

P

V

V

A

A

E

K

R

E

L

L

S

Q

N

S

L

L

L

L

N

G

K

K

P

D

V

V

T

T

F

F

V

L

P

N

V

D

T

C

E

V

G

G

K

P

Q

E

L

V

E

E

D

A

A

A

I

V

N

K

N

A

M

S

N

A

D

P

G

G

D

S

V

V

L

I

V

L

M

L

E

E

N

N

T

L

R
|
R

Y

Y

E

H

D

I

V

E

K

E

-

E

-

G

-

S

-

R

-

K

-

V

N

D

G

G

E

Q

Q

K

V

V

K

K

R

A

E

S

S

K

K

E

N

D

D

V

P

Q

A

K

L

F

G

R

K

H

Y

E

W

L

A

S

S

S

L

L

G

A

D

D

V

V

F

Y

I

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

Q

A

H

H

A

S

S

S

N

M

V

V

G

G

I

F

A

-

S

-

N

D

M

L

P

P

Q

Q

T

R

A

A

A

A

G

G

F

F

L

L

M

L

E

E

K

K

E

E

I

L

K

K

F

Y

I

F

G

G

G

K

A

A

V

L

S

E

H

N

P

P

A

T

H

R

P

P

F

F

V

L

A

A

I

I

L

L

G

G

G

G

A

A

K

K

V

V

S

A

D

D

K

K

I

I

G

Q

V

L

I

I

D

D

N

N

L

L

L

L

D

D

K

K

A

V

D

D

K

S

V

I

I

I

I

I

G

G

G

G

G

G

M

M

T

A

Y

F

T

T

F

F

Y

K

A

K

A

V

-

L

K

E

G

N

M

T

K

E

I

I

G

G

N

D

S

S

L

I

V

F

E

D

K

K

D

A

K

G

I

A

D

E

L

I

A

V

K

P

E

K

I

L

M

M

D

E

K

K

A

A

-

K

-

A

-

K

G

G

D

V

K

E

L

V

V

V

L

L

P

P

V

V

D

D

S

F

V

I

V

I

A

A

E

D

K

A

F

F

D

S

N

A

D

D

A

A

A

N

H

T

K

K

T

T

V

V

-

T

-

D

-

K

E

E

G

G

D

-

I

I

P

P

D

A

G

G

Y

W

M

Q

A

G

L

L

D

D

I

N

G

G

P

P

K

E

S

S

I

R

A

K

Q

L

F

F

E

A

D

A

V

T

L

V

K

A

S

K

A

A

K

K

L

T

V

I

V

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

F

F

E

E

M

F

S

E

N

K

Y

F

A

A

E

A

G

G

T

T

L

K

E

A

I

L

G

L

K

D

Y

E

L

V

G

V

T

K

L

S

T

S

D

A

A

A

-

G

-

N

T

T

T

V

I

I

V

I

G

G

G

G

G

G

D

D

S

T

T

A

A

T

A

V

V

A

Q

K

Q

K

L

Y

G

G

V

V

G

T

D

D

K

K

L

I

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

V

A

A

A

F

I

L

S

S

E

E

K

K

R22 (= R19) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R36) binding substrate
R122 (= R119) binding substrate
R169 (= R160) binding substrate

2paaA Crystal structure of phosphoglycerate kinase-2 bound to atp and 3pg (see paper)
47% identity, 99% coverage: 2:398/400 of query aligns to 1:411/413 of 2paaA

query
sites
2paaA

A

A

K

K

L

L

T

T

V

L

S

D

D

K

L

V

D

D

L

L

K

K

D

G

K

K

K

R

V

V

L

I

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

I

M

K

K

N

N

G

N

V

Q

I

I

G

T

N

N

D

N

N

Q

R
|
R

I

I

V

K

A

A

A

A

L

I

P

P

T

S

I

I

K

K

Y

H

V

C

I

L

E

D

H

N

D

G

G

A

K

K

A

S

I

V

-

V

L

L

L

M

S

S

H

H

L

L

G

G

R

R

V

P

K</