SitesBLAST

R26 (≠ G18) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P46) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F79) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y82) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ T84) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (= Y86) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ V87) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ L90) mutation to L: No effect on phenylalanine transport activity.
F101 (≠ Y93) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W97) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (≠ N99) mutation to L: No effect on phenylalanine transport activity.
W108 (≠ V100) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ Y103) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E110) mutation E->G,L,V,N: Loss of activity.
K168 (= K160) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E220) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R246) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P335) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
37% identity, 100% coverage: 1:460/462 of query aligns to 1:452/457 of P15993

query
sites
P15993

M

M

K

M

R

E

K

G

V

Q

K

Q

K

H

S

G

P

E

A

Q

L

L

R

K

R

R

S

G

M

L

T

K

A

N

G

R

Q

H

M

I

E

Q

M

L

I

I

S

A

L

L

G

G

A

A

I

I

G

G

V

T

G

G

L

L

F

F

M

L

G

G

S

S

T

A

S

S

T

V

I

I

K

Q

W

S

T

A

G

G

P

P

S

G

V

I

L

I

L

L

A

G

Y

Y

M

A

F

I

V

A

G

G

L

F

I

I

L

A

Y

F

I

L

V

I

M

M

R

R

A

Q

L

L

G

G

E

E

M

M

I

V

Y

V

V

E

N

E

P

P

G

V

T

A

G

G

S

S

F

F

A

S

D

H

Y

F

A

A

T

Y

E

K

Y

Y

V

W

H

G

P

S

L

F

A

A

G

G

Y

F

L

A

A

S

K

G

W

W

A

N

N

Y

V

W

F

V

E

L

Y
|
Y

I

V

V

L

V

V

G

A

M

M

S

A

E

E

V

L

V

T

A

A

A

V

T

G

Q

K

Y

Y

L

I

Q

Q

Y

F

W

W

W

Y

P

P

H

E

I

I

N

P

T

T

F

W

T

V

V

S

G

A

V

A

I

V

I

F

A

V

F

V

L

I

V

N

A

A

A

I

N

N

L

L

A

T

S

N

A

V

K

K

A

V

Y

F

G

G

S

E

L

M

E

E

F

F

W

W

F

F

A

A

M

I

I

I

K

K

V

V

I

I

T

A

I

V

M

A

M

M

I

I

L

F

G

G

L

G

L

W

V

L

I

L

F

F

F

S

G

G

L

-

G

-

N

N

G

G

H

P

P

Q

V

A

G

T

F

V

S

S

N

N

L

L

W

W

S

D

H

Q

G

G

F

F

F

L

T

P

G

H

G

G

V

F

K

T

G

G

F

L

F

V

F

M

S

M

M

M

S

A

I

I

V

M

G

F

S

S

Y

F

E

G

G

G

I

L

E

E

L

L

L

V

G

G

I

I

T

T

A

A

G

A

E

E

V

A

A

D

N

N

P

P

Q

E

K

Q

A

S

I

I

V

P

K

K

S

A

V

T

K

N

S

Q

V

V

L

I

F

Y

R

R

I

I

L

L

I

I

F

F

Y

Y

V

I

G

G

A

S

I

L

F

A

V

V

I

L

V

L

T

S

I

L

Y

M

P

P

W

W

N

T

R

R

L

V

S

T

A

A

I

D

G

T

S

S

P

P

F

F

V

V

S

L

T

I

F

F

A

H

K

E

V

L

G

G

I

D

T

T

A

F

A

V

A

A

S

N

V

A

I

L

N

N

F

I

V

V

L

L

T

T

A

A

L

L

S

S

S

V

A

Y

N

N

S

S

G

C

I

V

Y

Y

S

C

S

N

S

S

R

R

M

M

L

L

F

F

K

G

L

L

A

A

H

Q

E

Q

S

G

D

N

A

A

P

P

K

K

I

A

F

L

G

A

R

S

L

V

S

D

K

K

R

R

V

G

V

V

P

P

D

V

A

N

A

T

I

I

L

L

G

-

I

-

S

V

S

S

G

A

I

L

F

V

L

T

G

A

F

L

L

C

L

V

D

L

I

I

I

N

F

Y

S

L

A

A

Y

P

N

E

K

S

S

A

T

F

S

G

D

L

L

L

F

M

V

A

V

L

V

V

F

V

S

S

S

A

S

L

V

V

L

-

P

-

G

-

M

-

I

I

P

N

W

W

F

A

V

M

I

I

L

S

L

L

A

A

E

H

L

M

R

K

F

F

R

R

K

R

N

A

N

K

A

Q

I

E

V

Q

M

G

K

V

D

V

H

T

P

R

F

F

K

P

L

A

P

L

L

L

Y

Y

P

P

F

L

S

G

N

N

Y

W

F

I

A

C

M

L

F

L

V

F

L

M

V

A

I

V

V

L

G

V

F

I

M

M

F

L

V

M

N

T

P

P

D

G

T

M

R

A

V

I

S

S

V

V

I

Y

V

L

G

I

A

P

A

V

V

W

L

L

V

I

V

V

A

L

T

G

L

I

F

G

Y

Y

V

L

V

F

R

K

H

E

R

K

K

T

G

A

R

K

Y103 (= Y103) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
36% identity, 98% coverage: 6:458/462 of query aligns to 3:450/469 of P46349

query
sites
P46349

K

Q

K

S

S

Q

P

S

A

G

L

L

R

K

S

E

M

L

T

K

A

T

G

R

Q

H

M

M

E

T

M

M

I

I

S

S

L

I

G

A

G

G

A

V

I

I

G

G

V

A

G

G

L

L

F

F

M

V

G
|
G

S

S

T

G

S

S

T

V

I

I

K

H

W

S

T

T

G
|
G

P

P

S

G

V

A

L

V

A

S

Y

Y

M

A

F

L

V

A

G

G

L

L

I

L

L

V

Y

I

I

F

V

I

M

M

R

R

A

M

L

L

G

G

E

E

M

M

I

S

Y

A

V

V

N

N

P

P

G

T

T

S

G

G

S

S

F

F

A

S

D

Q

Y

Y

A

A

T

H

E

D

Y

A

V

I

H

G

P

P

L

W

A

A

G

G

Y

F

L

T

A

I

K

G

W

W

A

L

N

Y

V

W

F

F

E

F

Y

W

I

V

V

I

V

V

G

I

M

A

S

I

E

E

V

A

V

I

A

A

A

G

T

A

Q

G

Y

I

L

I

Q

Q

Y

Y

W

W

W

F

P

H

H

D

I

I

N

P

T

L

F

W

T

L

V

T

G

S

V

L

I

I

I

L

I

T

A

I

F

V

L

L

V

T

A

L

A

T

N

N

L

V

A

Y

S

S

A

V

K

K

A

S

Y

F

G

G

S

E

L

F

E

E

F

Y

W

W

F

F

A

S

M

L

I

I

K

K

V

V

I

V

T

T

I

I

M

A

M

F

I

L

I

I

L

V

G

G

L

F

L

A

V

F

I

I

F

F

F

-

G

G

L

F

G

A

N

P

G

G

G

S

H

E

P

P

V

V

G

G

F

F

S

S

N

N

L

L

W

T

S

G

H

K

G

G

F

F

T

P

G

E

G

G

V

I

K

S

G

S

F

V

F

L

S

G

M

I

S

V

I

V

V

I

G

F

S

S

Y

F

E

M

G

G

I

T

E

E

L

I

L

V

G

A

I

I

T

A

A

A

G

G

E

E

V

T

A

S

N

N

P

P

Q

I

K

E

A

S

I

V

V

T

K

K

S

A

V

T

K

R

S

S

V

V

L

V

F

W

R

R

I

I

L

I

I

V

F

F

Y

Y

V

V

G

G

A

S

I

I

F

A

V

I

I

V

V

V

T

A

I

L

Y

L

P

P

W

W

N

N

R

S

L

A

S

N

A

I

I

L

G

E

S

S

P

P

F

F

V

V

S

A

T

V

F

L

A

E

K

H

V

I

G

G

I

V

T

P

A

A

S

Q

V

I

I

M

N

N

F

F

V

I

V

V

L

L

T

T

A

A

A

V

L

L

S

S

S

C

A

L

N

N

S

S

G
|
G

I

L

Y

Y

S

T

S

S

R

R

M

M

L

L

F

Y

K

S

L

L

A

A

H

E

E

R

S

N

D

E

A

A

P

P

K

R

I

R

F

F

G

M

R

K

L

L

S

S

K

K

R

K

V

G

V

V

P

P

D

V

A

Q

A

A

I

I

L

V

G

-

I

-

S

-

S

A

G
|
G

I

T

F

F

L
x
F

G

S

F

Y

L

-

L

I

D

A

I

V

F

M

S

N

A

Y

Y

F

N

S

K

P

S

D

T

T

S

V

D

F

L

L

F

F

V

L

V

V

V

N

F

S

S

S

S

G

S

A

V

I

L

-

P

-

G

A

M

L

I

L

P

V

W

Y

F

L

V

V

I

I

L

A

L

V

A

S

E

Q

L

L

R

K

F

M

R

R

K

K

N

K

N

-

A

-

I

-

V

L

M

E

K

K

D

T

H

N

P

P

-

E

-

A

-

L

-

K

F

I

K

K

L

M

P

W

L

L

Y

F

P

P

F

F

S

L

N

T

Y

Y

F

L

A

T

M

I

F

I

V

A

L

I

V

C

-
x
G

V

I

I

L

V

V

G

S

F

M

M

A

F

F

V

I

N

D

P

S

D

M

T

R

R

D

V

E

S

L

V

L

I

L

V

T

G

G

A

V

A

I

V

T

L

G

V

I

V

V

A

L

T

I

L

S

F

Y

Y

L

V

V

V

F

R

R

H

K

R

R

K

K

G33 (= G36) mutation to D: Lack of activity.
G42 (= G45) mutation to S: Lack of activity.
G301 (= G305) mutation to V: Lack of activity.
G338 (= G345) mutation to E: Lack of activity.
F341 (≠ L348) mutation to S: Lack of activity.
G414 (vs. gap) mutation to R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
36% identity, 92% coverage: 2:425/462 of query aligns to 4:431/489 of P25737

query
sites
P25737

K

E

R

T

K

K

V

T

K

T

K

E

S

A

P

P

A

G

L

L

R

R

S

E

M

L

T

K

A

A

G

R

Q

H

M

L

E

T

M

M

I

I

S

A

L

I

G

G

A

S

I

I

G

G

V

T

G

G

L

L

F

F

M

V

G

A

S

S

T

G

S

A

T

T

I

I

K

S

W

Q

T

A

G

G

P

P

S

G

-

G

V

A

L

L

A

S

Y

Y

M

M

F

L

V

I

G

G

L

L

I

M

L

V

Y

Y

I

F

V

L

M

M

R

T

A

S

L

L

G

G

E

E

M

L

I

A

Y

A

V

Y

N

M

P

P

G

V

T

S

G

G

S

S

F

F

A

A

D

T

Y

Y

A

G

T

Q

E

N

Y

Y

V

V

H

E

P

E

L

G

A

F

G

G

Y

F

L

A

A

L

K

G

W

W

A

N

N
x
Y

V

W

F

Y

E

N

Y
x
W

I

A

V

V

V

T

G

I

M

A

S

V

E

D

V

L

V

V

A

A

T

Q

Q

L

Y

V

L

M

Q

S

Y

W

W

W

W

F

P

P

H

D

I

T

N

P

T

G

F

W

T

I

V

W

G

S

V

A

I

L

I

F

I

L

A

G

F

V

L

I

V

F

A

L

N

N

L

Y

A

I

S

S

A

V

K

R

A

G

Y

F

G

G

S

E

L

A

E

E

F

Y

W

W

F

F

A

S

M

L

I

I

K
|
K

V

V

I

T

T

T

I

V

I

I

M

V

M

F

I

I

L

V

G

G

L

V

L

L

V

M

I

I

F

-

F

I

G

G

L

I

G

F

N

K

G

G

G

A

H

Q

P

P

V

A

G

G

F

W

S

S

N

N

L

-

W

W

S

T

H

I

G

G

-

E

G

A

F

P

F

F

T

A

G

G

V

F

K

A

G

A

F

-

F

M

F

I

S

G

M

V

S

A

I

M

I

I

V

V

G

G

-
x
F

S

S

Y

F

E

Q

G

G

I

T

E
|
E

L

L

L

I

G

G

I

I

T

A

A

A

G

G

E
|
E

V

S

A

E

N

D

P

P

Q

A

K

K

A

N

I

I

V

P

K

R

S

A

V

V

K

R

S

Q

V

V

L

F

F

W

R

R

I

I

L

L

I

L

F

F

Y

Y

V

V

G

F

A

A

I

I

F

L

V

I

I

I

V

S

T

L

I

I

Y

I

P

P

W

Y

N

T

R

D

L

P

S

S

A

L

I

L

G

R

-

N

-
x
D

-

V

-

K

-
x
D

-

I

-

S

-

V

S

S

P

P

F

F

V

T

S

L

T

V

F

F

A

Q

K

H

V

A

G

G

I

L

T

L

A

S

A

A

S

A

V

V

I

M

N

N

F

A

V

V

V

I

L

L

T

T

A

A

A

V

L

L

S

S

S

A

A

G

N

N

S

S

G

G

I

M

Y

Y

S

A

S

S

S

T

R

R

M

M

L

L

F

Y

K

T

L

L

A

A

H

C

E

D

S

G

D

K

A

A

P

P

K

R

I

I

F

F

G

A

R

K

L

L

S

S

K

R

R

G

V

G

V

V

P

P

D

R

A

N

A

A

I

L

L

Y

G

A

I

T

S

T

S

V

G

I

I

A

F

G

L

L

G

C

F

F

L

L

L

T

D

S

I

M

I

F

F

-

S

-

A

-

Y

-

N

-

K

-

S

G

T

N

S

Q

D

T

L

V

F

Y

V

L

V

W

V

L

F

L

S

N

S

T

S

S

V

G

L

M

P

T

G

G

M

F

I

I

P

A

W

W

F

L

V

G

I

I

L

A

L

I

A

S

E

H

L

Y

R

R

F

F

R

R

K

R

N

G

N

Y

A

V

I

L

V

Q

-

G

-

H

-

D

M

I

K

N

D

D

H

L

P

P

F

Y

K

R

L

S

P

G

L

F

Y

F

P

P

F

L

S

G

N

P

Y

I

F

F

A

A

M

-

F

F

V

I

L

L

V

C

V

L

I

I

V

I

Y102 (≠ N99) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (≠ Y103) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K160) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (vs. gap) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E220) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E228) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 92% coverage: 7:432/462 of query aligns to 80:520/590 of P04817

query
sites
P04817

K

Q

S

N

P

A

A

E

L

V

R

K

R

R

S

E

M

L

T

K

A

Q

G

R

Q

H

M

I

E

G

M

M

I

I

S

A

L

L

G

G

A

T

I

I

G

G

V

T

G

G

L

L

F

F

M

I

G

G

S

L

T

S

S

T

T
x
P

I

L

K

T

W

N

T

A

G

G

P

P

-

V

S

G

V

A

L

L

L

I

A

S

Y

Y

M

L

F

F

V

M

G

G

L

S

I

L

L

A

Y

Y

I

S

V

V

M

T

R

Q

A

S

L

L

G

G

E

E

M

M

I

A

Y

T

V

F

N

I

P
|
P

G
x
V

T

T

G

S

S
|
S

F

F

A

T

D

V

Y

F

A

S

T

Q

E

R

Y

F

V

L

H

S

P

P

L

A

A

F

G

G

Y

A

L

A

A

N

K

G

W

Y

A

M

N
x
Y

V

W

F

F

E

S

Y

W

I

A

V

I

V

T

G

F

M

A

S

L

E

E

V

L

V

S

A

V

T

G

Q

Q

Y

V

L

I

Q

Q

Y

F

W

W

W

T

P

Y

H

K

I

V

N

P

T

L

F

A

T

A

V

W

G

I

V

S

I

I

I

F

I

W

A

V

F

I

L

I

V

T

A

I

A

M

N

N

L

L

A

F

S

P

A

V

K

K

A

Y

Y

Y

G

G

S

E

L

F

E

E

F

F

W

W

F

V

A

A

M

S

I

I

K

K

V

V

I

L

T

A

I

I

M

G

M

F

I

L

I

I

L

Y

G

C

L

F

L

C

V

M

I

V

F

C

F

-

G

G

L

A

G

G

N

V

G

T

G

G

H

-

P

P

V

V

G

G

F

F

S

-

N

R

L

Y

W

W

S

R

H

N

G

P

G

G

F

A

F

W

T

G

G

P

G

G

V

I

-

I

-

S

-

K

-

D

-

K

-

N

K

E

G

G

F

R

F

F

F

L

-

G

-

W

-

V

-

S

S

S

M

L

S

I

I

N

I

A

V

A

G

F

S

T

Y

F

E

Q

G

G

I

T

E

E

L

L

L

V

G

G

I

I

T

T

A

A

G
|
G

E

E

V

A

A

A

N

N

P
|
P

Q

R

K

K

A

S

I

V

V

P

K

R

S

A

V

I

K

K

S

K

V

V

L

V

F

F

R

R

I

I

L

L

I

T

F

F

Y

Y

V

I

G

G

A

S

I

L

F

L

V

F

I

I

V

G

T

L

I

L

Y

V

P

P

W

Y

N

N

R

D

L

P

S

K

A

L

I

T

G

Q

-

S

-
x
T

-
x
S

-
x
Y

-

V

-

S

-

T

S

S

P

P

F

F

V

I

S

I

T

A

F

I

A

E

K

N

V

S

G

G

I

T

T

K

A

V

A

L

A

P

S

H

V

I

I

F

N

N

F

A

V

V

V

I

L

L

T

T

A

T

A

I

L

I

S

S

S

A

A

A

N

N

S

S

G

N

I

I

Y

Y

S

V

S

G

S

S

R

R

M

I

L

L

F

F

K

G

L

L

A

S

H

K

E

N

S

K

D

L

A

A

P

P

K

K

I

F

F

L

G

S

R

R

L

T

S

T

K

K

R

G

V

G

V

V

P

P

D

Y

A

I

A

A

I

V

L

F

G

V

I

T

S

A

G

F

I

G

F

A

L

L

G

A

F

Y

L

M

L

-

D

-

I

-

F

-

S

-

A

E

Y

T

N

S

K

T

S

G

T

G

S

D

D

K

L

V

F

F

V

E

V
x
W

V

L

F

L

S

N

S

I

S

T

V

G

L

V

P

A

G

G

M
x
F

I

F

P

A

W

W

F

L

V

F

I

I

L

S

L

I

A

S

E

H

L

I

R

R

F

F

R

M

-

Q

-

A

-

L

K

K

N

Y

N

R

A

G

I

I

V

S

M

R

K

D

D

E

H

L

P

P

F

F

K

K

L

A

P

K

L

L

Y

M

P

P

F

G

S

L

N

A

Y

Y

F

Y

A

A

-

A

-

T

-

F

M

M

F

T

V

I

L

I

V

I

I

I

V

Q

G

G

F

F

M

T

F

A

V

F

N

A

P

P

P113 (≠ T40) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P74) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ G75) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S78) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (≠ N99) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (= G227) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P232) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ V369) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ M379) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
31% identity, 90% coverage: 11:424/462 of query aligns to 86:509/602 of P19145

query
sites
P19145

L

L

R

K

R

H

S

H

M

L

T

K

A

N

G

R

Q

H

M

L

E

Q

M

M

I

I

S

A

L

I

G

G

A

A

I

I

G

G

V

T

G

G

L

L

F

L

M

V

G

G

S

S

T

G

S

T

T

A

I

L

K

R

W

T

T

G

G

G

P

P

-

A

S

S

V

L

L

L

L

I

A

G

Y

W

M

G

F

S

V

T

G

G

L

T

I

M

L

I

Y

Y

I

A

V

M

M

V

R

M

A

A

L

L

G

G

E

E

M

L

I

A

Y

V

V

I

N

F

P

P

G

I

T

S

G

G

S

G

F

F

A

T

D

T

Y

Y

A

A

T

T

E

R

Y

F

V

I

H

D

P

E

L

S

A

F

G

G

Y

Y

L

A

A

N

K

N

W

F

A

N

N

Y

V

M

F

L

E

Q

Y

W

I

L

V

V

G

L

M

P

S

L

E

E

V

I

V

V

A

S

A

A

T

S

Q

I

Y

T

L

V

Q

N

Y

F

W

W

W

G

-

T

-

D

P

P

H

K

I

Y

N

R

T

D

F

G

T

F

V

V

G

A

V

L

I

F

I

W

I

L

A

A

F

-

L

I

V

V

A

I

N

N

L

M

A

F

S

G

A

V

K

K

A

G

Y

Y

G

G

S

E

L

A

E

E

F

F

W

V

F

F

A

S

M

F

I

I

K

K

V

V

I

I

T

T

I

V

M

G

M

F

I

I

L

L

G

G

L

-

V

I

I

I

F

L

F

N

G

C

L

G

G

G

N

G

G

P

G

T

H

G

P

G

V

Y

G

I

F

G

S

G

N

K

L

Y

W

W

S

H

H

D

G

P

G

G

F

A

F

F

T

A

G

G

G

D

V

T

K

P

G

G

F

A

F

K

F

F

S

K

-

G

-

V

M

C

S

S

I

V

I

F

V

V

G

T

-

A

-

F

S

S

Y

F

E
x
A

G

G

I

S

E

E

L

L

L

V

G

G

I

L

T

A

A

A

G

S

E

E

V

S

A

V

N

E

P

P

Q

R

K

K

A

S

I

V

V

P

K

K

S

A

V

A

K

K

S

Q

V

V

L

F

F

W

R

R

I

I

L

T

I

L

F

F

Y

Y

V

I

G

L

A

S

I

L

F

L

V

M

I

I

V

G

T

L

I

L

Y

V

P

P

W

Y

N

N

R

D

L

K

S

S

A

L

I

I

G

G

-

A

-

S

-

V

-

D

-

A

S

S

P

P

F

F

V

V

S

I

T

A

F

I

A

K

K

T

V

H

G

G

I

I

T

K

A

G

A

L

A

P

S

S

V

V

I

V

N

N

F

V

V

V

V

I

L

L

T

I

A

A

A

V

L

L

S

S

S

V

A

G

N

N

S

S

G

A

I

I

Y

Y

S

A

S

C

S

S

R

R

M

T

L

M

F

V

K

A

L

L

A

A

H

E

E

Q

S

R

D

F

A

L

P

P

K

E

I

I

F

F

G

S

R

Y

L

V

S

D

K

R

R

K

V

G

V

R

P

P

D

-

A

-

I

L

L

V

G

G

I

I

S

A

S

V

G

T

I

S

F

A

L

F

G

G

F

L

L

-

D

-

I

I

I

A

F

F

S

V

A

A

Y

A

N

S

K

K

S

K

T

E

S

G

D

E

L

V

F

F

V

N

V

W

V

L

F

L

S

A

S

L

S

S

V

G

L

L

P

S

G

S

M

L

I

F

P

T

W

W

F

G

V

G

I

I

L

C

L

I

A

C

E

H

L

I

R

R

F

F

R

R

K

K

N

A

N

L

A

A

I

A

V

Q

-

G

-

R

-

G

M

L

K

D

D

E

H

L

P

S

F

F

K

K

L

S

P

P

L

T

Y

G

P

V

F

W

S

G

N

S

Y

Y

F

W

A

G

M

L

F

F

V

M

L

V

V

I

I

M

A297 (≠ E217) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 88% coverage: 2:409/462 of query aligns to 71:485/587 of Q9URZ4

query
sites
Q9URZ4

K

K

R

A

K

T

V

G

K

E

K

D

S

G

P

T

A

A

L

L

R

K

R

R

S

S

M

L

T

K

A

S

G

R

Q

H

M

M

E

Q

M

M

I

I

S

S

L

I

G

G

A

A

I

I

G

G

V

T

G

G

L

L

F

Y

M

V

G

G

S

S

T

G

S

S

T

S

I

L

K

A

W

D

T

G

G

G

P

P

-

A

S

S

V

V

L

I

A

N

Y

Y

M

S

F

L

V

I

G

G

L

I

I

M

L

M

Y

F

I

F

V

I

M

V

R

Y

A

A

L

L

G

G

E

E

M

M

I

A

Y

V

V

A

N

Y

P

P

G

V

T

A

G

G

S

G

F

F

A

N

D

T

Y

Y

A

A

T

T

E

R

Y

F

V

I

H

D

P

P

L

A

A

W

G

G

Y

F

L

A

A

V

K

S

W

W

A

N

N

Y

V

F

F

I

E

N

Y

Y

I

F

V

V

V

T

G

F

M

P

S

L

E

E

V

L

V

T

A

T

A

C

T

A

Q

I

Y

T

L

F

Q

R

Y

Y

W

-

W

W

P

T

H

D

I

I

N

N

T

S

F

A

T

A

V

W

G

I

V

S

I

I

I

F

I

L

A

V

F

V

L

I

V

I

A

I

A

V

N

N

L

L

A

F

S

G

A

V

K

R

A

A

Y

Y

G

G

S

E

L

V

E

E

F

F

W

I

F

L

A

S

M

T

I

V

K

K

V

V

I

V

T

A

I

T

I

F

M

G

M

F

I

I

L

L

G

A

L

I

V

I

I

-

F

-

G

-

L

-

G

N

N

C

G

G

H

V

P

P

V

T

G

-

F

-

S

-

N

-

L

-

W

-

S

D

H

H

G

R

G

G

F

Y

-

I

-

G

-

S

-

I

-

K

-

H

-

K

-

P

F

F

T

R

G

H

G

G

V

F

K

K

G

G

F

F

F

C

F

S

S

V

M

F

S

T

I

T

I

A

V

A

G

F

S

S

Y

F

E

S

G

G

I

T

E

E

L

V

L

I

G

G

I

L

T

A

A

A

G

A

E

E

V

V

A

D

N

N

P

P

Q

Q

K

K

A

A

I

L

V

P

K

H

S

A

V

V

K

K

S

Q

V

V

L

F

F

W

R

R

I

I

L

A

I

I

F

F

Y

Y

V

V

G

V

A

S

I

L

F

I

V

L

I

I

V

G

T

L

I

L

Y

I

P

S

W

P

N

D

R

D

L

P

S

N

A

L

I

M

G

G

-

N

-

G

-

S

-

T

-

S

-

V

S

S

P

P

F

F

V

V

S

L

T

A

F

I

A

K

K

E

V

A

G

N

I

I

T

K

A

G

A

L

A

P

S

S

V

V

I

F

N

N

F

A

V

V

V

I

L

I

T

I

A

S

A

V

L

V

S

S

S

V

A

T

N

N

S

S

G

S

I

T

Y

Y

S

T

S

A

S

G

R

R

M

T

L

L

F

H

K

G

L

M

A

A

H

N

E

L

S

K

D

Q

A

A

P

P

K

S

I

F

F

F

G

K

R

Y

L

T

S

D

K

R

R

L

V

G

V

R

P

P

D

L

A

L

A

A

I

M

L

I

G

V

I

V

S

-

G

-

I

L

F

L

L

F

G

G

F

F

L

F

L

A

D

Y

I

I

I

-

F

-

S

N

A

E

Y

A

N

D

K

K

S

N

T

G

S

N

D

D

L

V

F

S

V

D

V

T

V

V

F

F

S

N

-

W

-

L

-

A

S

L

S

S

V

G

L

L

P

S

G

N

M

F

I

F

P

T

W

W

F

G

V

S

I

I

L

C

L

L

A

C

E

H

L

I

R

I

F

F

R

R

-

L

-

A

-

F

K

K

N

K

N

Q

A

G

I

H

V

S

M

L

K

K

D

E

H

L

P

G

F

F

K

V

L

S

P

P

L

M

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 85% coverage: 2:394/462 of query aligns to 136:535/663 of P48813

query
sites
P48813

K

Q

R

E

K

Q

V

E

K

Q

K

K

S

Q

P

E

A

N

L

L

R

K

S

S

M

I

T

K

A

P

G

R

Q

H

M

T

E

V

M

M

I

M

S

S

L

L

G

G

G

T

A

G

I

I

G

G

V

T

G

G

L

L

F

L

M

V

G

G

S

N

T

S

S

K

T

V

I

L

K

N

W

N

T

A

G

G

P

P

S

G

-

G

V

L

L

I

A

G

Y

Y

M

A

F

I

V

M

G

G

L

S

I

C

L

V

Y

Y

I

C

V

I

M

I

R

Q

A

A

L

C

G

G

E

E

M

L

-

A

-

V

I

I

Y

Y

V

S

N

D

P

L

G

-

T

I

G

G

S

G

F

F

A

N

D

T

Y

Y

A

P

T

L

E

F

Y

L

V

V

H

D

P

P

L

A

A

L

G

G

Y

F

L

S

A

V

K

A

W

W

A

L

N

F

V

C

F

L

E

Q

Y

W

I

L

V

C

V

V

G

C

M

P

S

L

E

E

V

L

V

V

A

T

A

A

T

S

Q

M

Y

T

L

I

Q

K

Y

Y

W

W

W

T

P

T

H

S

I

V

N

N

T

P

F

D

T

V

V

F

G

V

V

V

I

I

I

F

I

Y

A

V

F

L

L

I

V

V

A

V

A

I

N

N

L

V

A

F

S

G

A

A

K

K

A

G

Y

Y

G

A

S

E

L

A

E

D

F

F

W

F

F

F

A

N

M

C

I

C

K

K

V

I

I

L

T

M

I

I

I

V

M

G

M

F

I

F

I

I

L

L

G

A

L

I

V

I

I

D

F

C

F

G

G

G

L

A

G

G

N

T

G

D

G

G

H

Y

P

-

V

I

G

G

F

-

S

S

N

K

L

Y

W

W

S

R

H

D

G

P

G

G

F

A

F

F

T

R

G

G

G

D

V

T

-

P

-

I

-

Q

-

R

-

F

K

K

G

G

F

V

F

A

S

T

M

F

S

V

I

T

I

A

V

A

G

F

S

A

Y

F

E

G

G

M

I

S

E

E

L

Q

L

L

G

A

I

M

T

T

A

A

G

S

E

E

V

Q

A

S

N

N

P

P

Q

R

K

K

A

A

I

I

V

P

K

S

S

A

V

A

K

K

S

K

V

M

L

I

F

Y

R

R

I

I

L

L

I

F

F

V

Y

F

V

L

G

A

A

S

I

L

F

T

V

L

I

V

V

G

T

F

I

L

Y

V

P

P

W

Y

N

T

R

S

L

D

S

Q

A

L

I

L

G

G

-

A

-

G

-

S

-

A

-

T

-

K

-

A

S

S

P

P

F

Y

V

V

S

I

T

A

F

V

A

S

K

S

V

H

G

G

I

V

T

R

A

V

A

P

S

H

V

F

I

I

N

N

F

A

V

V

V

I

L

L

T

L

A

S

A

V

L

L

S

S

S

V

A

A

N

N

S

G

G

A

I

F

Y

Y

S

T

S

S

R

R

M

I

L

L

F

M

K

S

L

L

A

A

H

K

E

Q

S

G

D

N

A

A

P

P

K

K

I

C

F

F

G

D

R

Y

L

I

S

D

K

R

R

E

V

G

V

R

P

P

D

A

A

A

I

M

L

L

G

-

I

-

S

V

S

S

G

A

I

L

F

F

L

-

G

G

F

V

L

-

D

-

I

I

I

A

F

F

S

C

A

A

Y

S

N

S

K

K

S

K

T

E

S

E

D

D

L

V

F

F

V

T

V

W

V

L

F

L

S

A

S

I

S

S

V

G

L

L

P

S

G

Q

M

L

I

F

P

T

W

W

F

I

V

T

I

I

L

C

L

L

A

S

E

H

L

I

R

R

F

F

R

R

K

R

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 91% coverage: 2:422/462 of query aligns to 268:761/852 of Q03770

query
sites
Q03770

K

R

R

S

K

R

V

A

K

T

K

R

S

K

P

Y

A

H

L

I

R

Q

R

R

S

K

M

L

T

K

A

V

G

R

Q

H

M

I

E

Q

M

M

I

L

S

S

L

I

G

G

G

A

A

C

I

F

G

S

V

V

G

G

L

L

F

F

M

L

G

T

S

S

T

G

S

K

T

A

I

F

K

S

W

I

T

A

G

G

P

P

-

F

S

G

V

T

L

L

A

G

Y

F

M

G

F

L

V

T

G

G

L

S

I

I

L

I

Y

L

I

A

V

T

M

M

R

L

A

S

L

F

G

T

E

E

M

L

I

S

Y

T

V

L

N

I

P

P

G

V

T

S

G

S

S

G

F

F

A

S

D

G

Y

L

A

A

T

S

E

R

Y

F

V

V

H

E

P

D

L

A

A

F

G

G

Y

F

L

A

A

L

K

G

W

W

A

T

N

Y

V

W

F

I

E

S

Y

C

I

M

V

L

V

A

G

L

M

P

S

A

E

Q

V

V

V

S

A

S

T
|
T

Q

F

Y

Y

L

L

Q

S

Y

Y

W

-

W

Y

P

N

H

N

I

V

N

N

T

I

-

S

-

K

-

G

F

V

T

T

V

A

G

G

V

F

I

I

I

T

I

L

-

F

-

S

A

A

F

F

L

S

V

I

A

V

N

N

L

L

A

L

S

D

A

V

K

S

A

I

Y

M

G

G

S

E

L

I

E

V

F

Y

W

V

F

A

A

G

M

I

I

S

K

K

V

V

I

I

T

I

I

A

I

I

M

L

M

M

I

V

I

F

L

T

G

-

L

M

L

I

V

I

I

L

F

N

F

A

G

G

L

H

G

G

N

N

G

D

G

I

H

H

P

E

-

G

V

V

G

G

F

F

S

-

N

R

L

Y

W

W

S

D

H

S

G

S

-

K

-

S

-

V

-

R

-

N

-

L

-

T

-

Y

G

G

F

L

F

Y

T

R

-

P

-

T

-

F

-

D

-

L

-

A

-

D

-

A

-

G

-

E

-

G

-

S

-

K

-

G

-

I

G

S

G

G

V

P

K

K

G

G

F

R

F

F

F

L

S

A

-

T

-

A

-

S

-

V

M

M

S

L

I

I

I

S

V

T

G

F

S

A

Y

F

E

S

G

G

I

V

E

E

L

M

L

T

G

F

I

L

T

A

A

S

G

G

E

E

V

A

A

I

N

N

P

P

Q

R

K

K

A

T

I

I

V

P

K

S

S

A

V

T

K

K

S

R

V

T

L

F

F

S

R

I

I

V

L

L

I

I

F

S

Y

Y

V

V

G

F

A

L

I

I

F

F

V

S

I

V

-

G

V

I

T

N

I

I

Y

Y

P

S

W

G

N

D

-

P

-

R

-

L

-

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

R

R

L

Y

S

E

A

A

I

I

-

I

-

K

-

G

-

T

-

G

-

M

-

D

-

W

-

R

-

L

-

R

-

T

-

N

-

C

-

R

-

G

-

I

-

D

-

Y

-

R

-

Q

-

I

-

S

-

V

-

G

-

T

-

G

-

Y

G

S

S

S

P

P

F

W

V

V

S

V

T

A

F

L

A

Q

K

N

V

F

G

G

I

L

T

C

A

T

A

F

A

A

S

S

V

A

I

F

N

N

F

A

V

I

V

L

L

I

T

F

A

F

A

T

L

A

S

T

S

A

A

G

N

I

S

S

G

S

I

L

Y

F

S

S

S

C

S

S

R

R

M

T

L

L

F

Y

K

A

L

M

A

S

H

V

E

Q

S

R

D

K

A

A

P

P

K

P

I

V

F

F

G

E

R

I

L

C

S

S

K

K

R

R

V

G

V

V

P

P

D

Y

A

V

A

S

I

V

L

-

G

-

I

-

S

-

G

-

I

I

F

F

L

S

G

S

F

L

L

F

L

S

D

V

I

I

I

A

F

Y

S

I

A

A

Y

V

N

D

K

Q

S

T

T

A

S

I

D

E

L

N

F

F

V

D

V

V

V

L

F

A

S

N

S

V

S

S

V

S

L

A

P

S

G

T

M

S

I

I

P

I

W

W

F

M

V

G

I

L

L

N

L

L

A

S

E

F

L

L

R

R

F

F

-

Y

-

A

-

L

-

K

R

Q

K

R

N

K

N

D

A

I

I

I

V

S

M

R

K

N

D

D

-

S

H

Y

P

P

F

Y

K

K

L

S

P

P

L

F

Y

Q

P

P

F

-

S

-

N

-

Y

Y

F

L

A

A

M

I

F

Y

V

G

L

L

V

V

T382 (= T115) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
22% identity, 94% coverage: 25:458/462 of query aligns to 22:436/438 of O34739

query
sites
O34739

L

I

G

G

G

T

A

I

I

I

G

G

V

S

G

G

L

V

F

F

M

M

G

K

S

P

T

G

S

A

T

V

I

L

K

A

W

Y

T

S

G

G

P

D

S

S

-

K

-

M

V

A

L

L

L

F

A

A

Y

W

M

L

F

L

V

G

G

G

L

I

I

L

L

T

Y

L

I

A

V

G

M

G

R

L

A

T

L

I

G

A

E

E

M

I

I

G

Y

T

V

Q

N

I

P

P

G

K

T

T

G

G

S

G

F

L

A

Y

D

T

Y

Y

A

L

T

E

E

E

Y

V

V

Y

H

G

P

E

L

F

A

W

G

G

Y

F

L

L

A
x
C

K

G

W

W

A

V

N

Q

V

I

F

I

E

I

Y

Y

-

G

-

P

-

A

I

I

V

I

V

G

G

A

M

L

S

G

E

L

V

Y

V

F

A

G

A

S

T

L

Q

M

Y

A

L

N

Q

L

Y

F

W

G

W

W

P

G

H

S

I

G

N

L

T

S

F

K

T

V

V

I

G

G

V

I

I

I

I

A

I

V

A

L

F

F

L

L

V
x
C

A

V

A

I

N

N

L

I

A

I

S

G

A

T

K

K

A

-

Y

Y

G

G

S

G

L

F

E

-

F

-

W

-

F

-

A

-

M

-

I

V

K

Q

V

T

I

L

T

T

I

T

I

I

M

G

M

K

I

L

I

I

L

P

G

I

L

A

L
x
C

V

I

I

I

F

V

F

F

G

G

L

L

G

W

N

K

G

G

G

D

H

Q

P

H

V

I

G

-

F

F

S

T

N

A

L

V

W

-

S

-

H

-

G

-

F

-

T

N

G

E

G

S

V

I

K

S

G

D

F

M

F

N

F

F

S

G

M

A

S

A

I

I

I

L

V

A

G

T

-

L

-

F

S

A

Y

Y

E

D

G

G

I

W

E

I

L

L

G

A

I

A

T

L

A

G

G

G

E

E

V

M

A

K

N

N

P

P

Q

E

K

K

A

L

I

L

V

P

K

R

S

A

V

M

K

T

S

G

V

G

L

L

F

L

R

I

I

V

L

T

I

A

F

I

Y

Y

V

I

G

F

A

I

I

N

F

F

V

A

I

L

V

L

T

H

I

I

Y

L

P

S

W

A

N

N

R

E

L

I

S

V

A

T

I

L

G

G

S

E

P

N

F

A

V

T

S

S

T

T

F

A

A

A

K

T

V

M

G

L

I

F

-

G

T

S

A

I

A

G

S

K

V

L

I

I

N

S

F

V

V

G

V

I

L

I

T

V

A

S

A

I

L

F

S

G

S
x
C

A

L

N

N

S

G

G

K

I

V

Y

L

S

S

S

F

S

P

R

R

M

V

L

S

F

F

K

A

L

M

A

A

H

E

E

R

S

K

D

Q

A

L

P

P

K

-

I

-

F

F

G

A

R

E

L

K

S

L

K

S

R

H

V

V

V

H

P

P

D

S

-

F

-

R

A

T

A

P

I

W

L

I

G

A

I

I

S

S

S

F

G

Q

I

I

F

A

L

L

G

A

F

L

L

I

L

M

D

M

I

L

I

I

F

S

S

N

A

P

Y

D

N

K

K

L

S

S

T

E

S

I

D

S

L

I

F

F

V

M

V

I

-

Y

V

I

F

F

S

Y

S

-

V

-

L

-

P

-

G

-

M

V

I

M

P

A

W

F

F

F

V

A

I

V

L

F

L

I

A

-

E

-

L

L

R

R

F

K

R

R

K

A

N

K

N

G

A

-

I

-

V

-

M

-

K

E

D

K

H

R

P

A

F

Y

K

S

L

V

P

P

L

L

Y

Y

P

P

F

F

S

M

N

P

Y

I

F

L

A

A

M

I

F

A

V

G

L

S

V

F

I

V

V

L

G

G

F

S

M

T

F

L

V

I

N

T

P

D

-

T

-

M

D

S

T
x
C

R

G

V

L

S

S

V

I

I

L

V

I

G

G

A

L

A

A

V

G

L

L

V

P

V

V

A

-

T

-

L

-

F

Y

Y

Y

V

G

V

M

R

K

H

K

R

R

K

K

C94 (≠ A95) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
C141 (≠ V139) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
C168 (≠ L173) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
C291 (≠ S301) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
C415 (≠ T434) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
21% identity, 58% coverage: 67:332/462 of query aligns to 74:336/461 of P76037

query
sites
P76037

G

G

E

K

M

L

I

V

Y

R

V

Q

N

F

P

P

G

E

T

A

G

G

S

S

F

A

A

Y

D

T

Y

Y

A

A

T

Q

E

K

Y

S

V

I

H

N

P

P

L

H

A

V

G

G

Y

F

L

M

A

V

K

G

W

W

A

S

N

S

V

L

F

L

E

D

Y
|
Y

I

L

V

F

V

L

G

P

M

M

S

I

E

N

V

V

V

L

A

L

A

A

T

K

Q

I

Y

Y

L

L

Q

S

Y

A

W

L

W

F

P

P

H

E

I

V

N

P

T

P

F

W

T

V

V

W

G

V

V

V

I

T

I

F

I

V

A

A

F

I

L

L

V

T

A

A

N

N

L

L

A

K

S

S

A

V

K

N

A

L

Y

V

G

A

S

N

L

F

E

N

F

T

W

L

F

F

A

V

M

L

I

V

K

Q

V

I

I

S

T

I

I

M

I

V

M

V

M

F

I

I

I

F

L

L

G

-

L

-

V

-

I

V

F

V

F

Q

G

G

L

L

G

H

N

K

G

G

G

E

H

-

P

-

V

-

G

G

F

V

S

G

N

T

L

V

W

W

S

S

H

L

G

Q

G

P

F

F

F

I

T

S

G

E

G

N

V

A

K

H

-

L

-

I

G

P

F

I

F

T

S

G

M

A

S

T

I

I

I

V

V

C

G

F

S

S

Y

F

E

L

G

G

I

F

E

D

L

A

L

V

G

T

I

T

T

L

A

S

G

E

E

E

V

T

A

P

N

D

P

A

Q

A

K

R

A

V

I

I

V

P

K

K

S

A

V

I

-

F

K

L

S

T

V

A

L

V

F

Y

R

G

I

G

L

V

I

I

F

F

Y

I

V

A

G

A

A

S

I

F

F

F

V

M

I

Q

V

L

T

F

I

F

Y

P

P

D

W

I

N

S

R

R

L

F

S

K

A

D

I

P

G

D

S

A

P

A

F

L

V

P

S

E

T

I

F

A

A

L

K

Y

V

V

G

G

I

G

T

K

A

L

A

F

A

Q

S

S

V

I

I

F

N

L