SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_089214750.1 NCBI__GCF_900188185.1:WP_089214750.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0DX84 3-methylmercaptopropionyl-CoA ligase; MMPA-CoA ligase; EC 6.2.1.44 from Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) (Silicibacter lacuscaerulensis) (see paper)
52% identity, 98% coverage: 3:525/534 of query aligns to 5:535/539 of P0DX84

query
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P0DX84

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H231 (= H223) mutation to A: Retains 74% of wild-type activity.
W235 (= W227) mutation to A: Almost completely abolishes the activity.
G302 (= G293) mutation to P: Almost completely abolishes the activity.
G303 (= G294) mutation to P: Almost completely abolishes the activity.
W326 (= W316) mutation to A: Retains 7.7% of wild-type activity.
P333 (= P323) mutation to A: Retains 69% of wild-type activity.
R432 (= R422) mutation to A: Retains 4.3% of wild-type activity.
K434 (= K424) mutation to A: Retains 36% of wild-type activity.
D435 (= D425) mutation to A: Retains 76% of wild-type activity.
K438 (= K428) mutation to A: Retains 5.6% of wild-type activity.
G440 (= G430) mutation to P: Retains 3.6% of wild-type activity.
G441 (= G431) mutation to P: Retains 2.7% of wild-type activity.
E442 (= E432) mutation to A: Retains 27% of wild-type activity.
W443 (= W433) mutation to A: Retains 60% of wild-type activity.
E474 (= E464) mutation to A: Retains 33% of wild-type activity.
K523 (= K513) Plays an important role in catalysis; mutation to A: Retains 1.6% of wild-type activity.; mutation to E: Retains 1.4% of wild-type activity.; mutation to R: Retains 57% of wild-type activity.
K526 (= K516) mutation to A: Retains 48% of wild-type activity.

6ijbB Structure of 3-methylmercaptopropionate coa ligase mutant k523a in complex with amp and mmpa (see paper)
51% identity, 98% coverage: 3:525/534 of query aligns to 5:535/538 of 6ijbB

query
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6ijbB

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active site: T185 (= T177), H205 (= H197), H231 (= H223), S329 (≠ T319), E330 (= E320), K438 (= K428), W443 (= W433), A523 (≠ K513)
binding 3-(methylsulfanyl)propanoic acid: W235 (= W227), G303 (= G294), A325 (= A315), W326 (= W316), G327 (= G317), M328 (= M318)
binding adenosine monophosphate: G303 (= G294), A304 (≠ S295), A305 (= A296), H324 (= H314), W326 (= W316), G327 (= G317), M328 (= M318), S329 (≠ T319), Q359 (= Q349), D417 (= D407)

6ihkB Structure of mmpa coa ligase in complex with adp (see paper)
51% identity, 98% coverage: 3:525/534 of query aligns to 5:532/533 of 6ihkB

query
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6ihkB

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E

-

F

Y

Y

R

D

D

E

L

L

V

I

E

E

A

T

E

G

D

D

G

T

R

D

F

F

E

E

W

W

V

P

E

V

L

F

D

D

E

E

N

N

D

T

P

A

V

S

G

S

L

L

C

C

Y

Y

T
|
T

S

S

G

G

T

-

G

-

N

H

P

P

K

K

G

G

V

V

L

L

Y

Y

E

S

H

H

R

R

S

S

N

T

V

V

I

L

H
|
H

A

S

I

F

T

A

E

S

I

N

Q

T

P

R

D

D

V

V

F

I

D

G

L

Y

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S

N

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R

M

A

D

V

V

L

M

P

P

I

V

V

V

P

P

M

M

F

F

H
|
H

A

V

N

N

S

A

W

W

G

G

V

S

P

P

Y

Y

A

G

A

C

A

A

T

M

V

S

G

G

A

A

K

Q

L

M

V

V

F

L

S

P

A

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N

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L

D

H

A

G

Q

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V

A

L

L

C

V

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N

L

L

M

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D

D

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Y

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V

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T

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L

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A

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W

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G

G

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G

K

Y

L

G

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K

S

L

L

R

E

R

R

V

T

I

V

I

I

G

G

G
|
G

S
x
A

A
|
A

A

C

P

P

R

P

A

S

M

M

I

I

E

A

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T

F

F

M

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-

E

R

K

A

Y

G

G

I

V

D

D

V

T

G

V

H
|
H

A
|
A

W
|
W

G
|
G

M
|
M

T
x
S

E
|
E

T

M

S

S

P

P

I

L

G

G

T

T

M

A

G

N

K

I

R

P

P

L

W

A

N

K

W

H

D

R

D

K

M

L

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F

I

D

E

E

E

Q

Q

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H

D

K

M

L

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C

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N

Q
|
Q

G

G

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P

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F

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V

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L

L

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D

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N

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L

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D

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L

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L

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D

D

G

W

W

F

F

D

A

T

T

G

G

D
|
D

V

V

S

A

V

T

I

L

H

D

P

P

D

D

G

G

V

Y

M

M

Q

T

I

I

T

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D

D

R
|
R

A

S

K

K

D

D

V

I

I

I

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|
K

S

S

G

G

E

E

W
|
W

I

I

S

S

I

V

E

E

L

L

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E

N

N

A

I

A

A

V

V

G

A

A

H

P

P

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K

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L

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A

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T

A

A

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V

I

G

G

V

V

Y

P

H

H

P

P

K

K

W

W

D

D

E

E

R

R

P

P

I

L

L

L

L

V

I

A

V

V

K

K

K

A

P

E

G

G

A

E

D

D

V

P

G

S

E

E

A

A

D

E

I

L

L

L

D

E

Y

F

L

F

K

D

D

G

K

K

V

I

A

A

K

K

W

W

W

Q

L

V

P

P

D

D

E

K

I

V

V

V

F

F

V

V

D

D

E

A

L

L

P

P

H

L

T

N

A

A

T

T

G

G

K
|
K

I

V

L

L

K

K

R

R

Q

K

I

L

R

R

D

D

D

E

Y

F

K

K

D

D

active site: T185 (= T177), H202 (= H197), H228 (= H223), S326 (≠ T319), E327 (= E320), K435 (= K428), W440 (= W433), K520 (= K513)
binding adenosine-5'-diphosphate: H228 (= H223), G300 (= G294), A301 (≠ S295), A302 (= A296), H321 (= H314), A322 (= A315), W323 (= W316), G324 (= G317), M325 (= M318), S326 (≠ T319), Q356 (= Q349), D414 (= D407), R429 (= R422), K520 (= K513)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
44% identity, 98% coverage: 3:526/534 of query aligns to 13:537/541 of Q5SKN9

query
sites
Q5SKN9

M

M

Q

M

G

D

Q

E

P

E

L

L

L

N

V

L

T

W

S

D

L

F

I

L

D

E

H

R

A

A

R

A

E

L

H

F

A

G

G

R

R

K

E

E

I

V

V

V

T

S

R

R

W

L

A

H

D

T

G

G

S

E

M

V

T

H

R

R

S

T

T

T

W

Y

G

A

E

E

V

V

G

Y

T

Q

D

R

A

A

R

R

F

L

A

M

A

G

L

L

T

R

K

A

L

L

G

G

M

V

K

G

K

V

G

G

D

D

R

R

I

V

A

A

T

T

L

L

A

G

M

F

N

N

H

H

G

F

H

R

H

H

L

L

V

E

S

A

W

Y

Y

F

G

A

T

V

A

P

G

G

M

M

G

G

G

A

V

V

L

L

H

H

T

T

V

A

N

N

P

P

R

R

L

L

F

S

D

P

E

K

Q

E

L

I

V

A

Y

Y

I

I

I

L

N

N

H

H

A

A

G

E

D

D

R

K

M

V

L

L

F

L

F

F

D

D

A

P

A

N

F

L

L

L

P

P

I

L

V

V

E

E

R

A

L

I

R

R

D

G

Q

E

L

L

P

K

T

T

V

V

E

Q

H

H

F

F

V

V

L

V

F

M

D

D

A

E

P

K

A

A

R

P

E

E

G

G

Y

Y

L

L

S

A

Y

Y

R

E

D

E

L

A

V

L

E

G

A

E

E

E

D

A

G

-

R

-

F

-

E

D

W

P

V

V

E

R

L

V

D

P

E

E

N

R

D

A

P

A

V

C

G

G

L

M

C

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

N

L

P

P

K

K

G

G

V

V

L

V

Y

Y

E

S

H

H

R

R

S

A

N

L

V

V

I

L

H

H

A

S

I

L

T

A

E

A

I

S

Q

L

P

V

D

D

V

G

F

T

D

A

L

L

S

S

N

E

R

K

A

D

V

V

L

L

P

P

I

V

V

V

P

P

M

M

F

F

H

H

A

V

N

N

S

A

W

W

G

C

V

L

P

P

Y

Y

A

A

A

T

T

L

V

V

G

G

A

A

K

K

L

Q

V

V

F

L

S

P

A

G

T

P

N

R

-

L

D

D

A

P

Q

A

V

S

L

L

C

V

D

E

L

L

M

F

H

D

D

G

E

E

G

G

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V

T

T

H

F

S

T

A

A

G

G

V

V

P

P

T

T

V

V

W

W

I

L

A

A

M

L

F

A

A

D

H

Y

M

L

D

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A

S

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T

G

G

I

H

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R

Y

L

G

K

K

T

L

L

R

R

V

L

I

V

G

G

G
|
G

S

S

A

A

P

P

R

R

A

S

M

L

I

I

E

A

R

R

F

F

M

E

R

R

A

M

G

G

I

V

D

E

V

V

G

R

H
x
Q

A
x
G

W

Y

G

G

M

L

T
|
T

E
|
E

T

T

S

S

P

P

I

V

G

V

T

V

M

Q

G

N

K

F

R

V

P

K

W

S

N

H

W

L

D

E

D

S

M

L

S

S

F

E

D

E

E

E

Q

K

V

L

D

T

M

L

V

K

C

A

R

K

Q

T

G

G

C

L

P

P

P

I

F

P

G

L

V

V

E

R

L

L

R

R

I

V

V

A

D

D

D

E

E

E

G

G

S

R

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P

L

V

P

P

R

K

D

D

G

G

R

K

T

A

S

L

G

G

R

E

L

V

Q

Q

I

L

R

K

G

G

P

P

W

W

I

I

I

T

R

G

Y

Y

F

Y

-

G

-

N

-

E

R

E

A

A

G

T

E

R

D

S

A

A

V

L

D

T

E

P

G

D

D

G

W

F

F

F

D

R

T

T

G

G

D
|
D

V

I

S

A

V

V

I

W

H

D

P

E

D

E

G

G

V

Y

M

V

Q

E

I

I

T

K

D

D

R

R

A

L

K
|
K

D

D

V

L

I

I

K
|
K

S

S

G

G

E

E

W

W

I

I

S

S

I

V

E

D

L

L

E

E

N

N

A

A

A

L

V

M

G

G

A

H

P

P

G

K

V

V

Q

K

E

E

A

A

A

V

V

V

G

A

V

I

Y

P

H

H

P

P

K

K

W

W

D

Q

E

E

R

R

P

P

I

L

L

A

L

V

I

V

V

V

K

P

K

R

P

G

G

E

A

K

D

P

V

T

G

P

E

E

A

E

D

L

I

N

L

E

D

H

Y

L

L

L

K

K

D

A

K

G

V

F

A

A

K

K

W

W

W

Q

L

L

P

P

D

D

E

A

I

Y

V

V

F

F

V

A

D

E

E

E

L

I

P

P

H

R

T

T

A

S

T

A

G

G

K

K

I

F

L

L

K

K

R

R

Q

A

I

L

R

R

D

E

D

Q

Y

Y

K

K

D

N

Y

Y

T184 (= T177) binding Mg(2+)
G302 (= G294) binding tetradecanoyl-AMP
Q322 (≠ H314) binding tetradecanoyl-AMP
G323 (≠ A315) binding tetradecanoyl-AMP
T327 (= T319) binding tetradecanoyl-AMP
E328 (= E320) binding Mg(2+)
D418 (= D407) binding tetradecanoyl-AMP
K435 (= K424) binding tetradecanoyl-AMP
K439 (= K428) binding tetradecanoyl-AMP

1v26B Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
42% identity, 98% coverage: 3:526/534 of query aligns to 6:506/510 of 1v26B

query
sites
1v26B

M

M

Q

M

G

D

Q

E

P

E

L

L

L

N

V

L

T

W

S

D

L

F

I

L

D

E

H

R

A

A

R

A

E

L

H

F

A

G

G

R

R

K

E

E

I

V

V

V

T

S

R

R

W

L

A

H

D

T

G

G

S

E

M

V

T

H

R

R

S

T

T

T

W

Y

G

A

E

E

V

V

G

Y

T

Q

D

R

A

A

R

R

F

L

A

M

A

G

L

L

T

R

K

A

L

L

G

G

M

V

K

G

K

V

G

G

D

D

R

R

I

V

A

A

T

T

L

L

A

G

M

F

N

N

H

H

G

F

H

R

H

H

L

L

V

E

S

A

W

Y

Y

F

G

A

T

V

A

P

G

G

M

M

G

G

G

A

V

V

L

L

H

H

T

T

V

A

N

N

P

P

R

R

L

L

F

S

D

P

E

K

Q

E

L

I

V

A

Y

Y

I

I

I

L

N

N

H

H

A

A

G

E

D

D

R

K

M

V

L

L

F

L

F

F

D

D

A

P

A

N

F

L

L

L

P

P

I

L

V

V

E

E

R

A

L

I

R

R

D

G

Q

E

L

L

P

K

T

T

V

V

E

Q

H

H

F

F

V

V

L

V

F

M

D

D

A

E

P

K

A

A

R

P

E

E

G

G

Y

Y

L

L

S

A

Y

Y

R

E

D

E

L

A

V

L

E

G

A

E

E

E

D

A

G

-

R

-

F

-

E

D

W

P

V

V

E

R

L

V

D

P

E

E

N

R

D

A

P

A

V

C

G

G

L

M

C

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

N

L

P

P

K

K

G

G

V

V

L

V

Y

Y

E

S

H

H

R

R

S

A

N

L

V

V

I

L

H
|
H

A

S

I

L

T

A

E

A

I

S

Q

L

P

V

D

D

V

G

F

T

D

A

L

L

S

S

N

E

R

K

A

D

V

V

L

L

P

P

I

V

V

V

P

P

M

M

F

F

H
|
H

A

V

N

N

S

A

W

W

G

C

V

L

P

P

Y

Y

A

A

A

T

T

L

V

V

G

G

A

A

K

K

L

Q

V

V

F

L

S

P

A

G

T

P

N

R

-

L

D

D

A

P

Q

A

V

S

L

L

C

V

D

E

L

L

M

F

H

D

D

G

E

E

G

G

V

V

T

T

H

F

S

T

A

A

G

G

V

V

P

P

T

T

V

V

W

W

I

L

A

A

M

L

F

A

A

D

H

Y

M

L

D

E

A

S

T

T

G

G

I

H

G

R

Y

L

G

K

K

T

L

L

R

R

V

L

I

V

G

G

G
|
G

S
|
S

A
|
A

A

A

P

P

R

R

A

S

M

L

I

I

E

A

R

R

F

F

M

E

R

R

A

M

G

G

I

V

D

E

V

V

G

R

H

Q

A
x
G

W
x
Y

G
|
G

M
x
L

T
|
T

E
|
E

T

T

S

S

P

P

I

V

G

V

T

V

M

Q

G

N

K

F

R

V

P

K

W

S

N

H

W

L

D

E

D

S

M

L

S

S

F

E

D

E

E

E

Q

K

V

L

D

T

M

L

V

K

C

A

R

K

Q

T

G

G

C

L

P

P

P

I

F

P

G

L

V

V

E

R

L

L

R

R

I

V

V

A

D

D

D

E

E

E

G

G

S

R

E

P

L

V

P

P

R

K

D

D

G

G

R

K

T

A

S

L

G

G

R

E

L

V

Q

Q

I

L

R

K

G

G

P

P

W

W

I

I

I

T

R

G

Y

Y

F

Y

-

G

-

N

-

E

R

E

A

A

G

T

E

R

D

S

A

A

V

L

D

T

E

P

G

D

D

G

W

F

F

F

D

R

T

T

G

G

D
|
D

V

I

S

A

V

V

I

W

H

D

P

E

D

E

G

G

V

Y

M

V

Q

E

I

I

T

K

D

D

R

R

A

L

K
|
K

D

D

V

L

I

I

K
|
K

S

S

G

G

E

E

W
|
W

I

I

S

S

I

V

E

D

L

L

E

E

N

N

A

A

A

-

V

-

G

-

A

-

P

-

G

-

V

-

Q

-

E

-

A

A

A

V

V

V

G

A

V

I

Y

P

H

H

P

P

K

K

W

W

D

Q

E

E

R

R

P

P

I

L

L

A

L

V

I

T

V

P

K

E

P

L

G

N

A

E

D

H

V

L

G

-

E

-

A

-

D

-

I

-

L

-

D

-

Y

-

L

L

K

K

D

A

K

G

V

F

A

A

K

K

W

W

W

Q

L

L

P

P

D

D

E

A

I

Y

V

V

F

F

V

A

D

E

E

E

L

I

P

P

H

R

T

T

A

-

T

-

G

-

K

-

I

-

L

-

K

-

R

R

Q

A

I

L

R

R

D

E

D

Q

Y

Y

K

K

D

N

Y

Y

active site: T177 (= T177), H197 (= H197), H223 (= H223), T320 (= T319), E321 (= E320), K432 (= K428), W437 (= W433)
binding adenosine monophosphate: G295 (= G294), S296 (= S295), A297 (= A296), G316 (≠ A315), Y317 (≠ W316), G318 (= G317), L319 (≠ M318), T320 (= T319), D411 (= D407), K428 (= K424), K432 (= K428), W437 (= W433)
binding magnesium ion: T177 (= T177), E321 (= E320)

1v25A Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
41% identity, 98% coverage: 3:526/534 of query aligns to 6:487/491 of 1v25A

query
sites
1v25A

M

M

Q

M

G

D

Q

E

P

E

L

L

L

N

V

L

T

W

S

D

L

F

I

L

D

E

H

R

A

A

R

A

E

L

H

F

A

G

G

R

R

K

E

E

I

V

V

V

T

S

R

R

W

L

A

H

D

T

G

G

S

E

M

V

T

H

R

R

S

T

T

T

W

Y

G

A

E

E

V

V

G

Y

T

Q

D

R

A

A

R

R

F

L

A

M

A

G

L

L

T

R

K

A

L

L

G

G

M

V

K

G

K

V

G

G

D

D

R

R

I

V

A

A

T

T

L

L

A

G

M

F

N

N

H

H

G

F

H

R

H

H

L

L

V

E

S

A

W

Y

Y

F

G

A

T

V

A

P

G

G

M

M

G

G

G

A

V

V

L

L

H

H

T

T

V

A

N

N

P

P

R

R

L

L

F

S

D

P

E

K

Q

E

L

I

V

A

Y

Y

I

I

I

L

N

N

H

H

A

A

G

E

D

D

R

K

M

V

L

L

F

L

F

F

D

D

A

P

A

N

F

L

L

L

P

P

I

L

V

V

E

E

R

A

L

I

R

R

D

G

Q

E

L

L

P

K

T

T

V

V

E

Q

H

H

F

F

V

V

L

V

F

M

D

D

A

E

P

K

A

A

R

P

E

E

G

G

Y

Y

L

L

S

A

Y

Y

R

E

D

E

L

A

V

L

E

G

A

E

E

E

D

A

G

-

R

-

F

-

E

D

W

P

V

V

E

R

L

V

D

P

E

E

N

R

D

A

P

A

V

C

G

G

L

M

C

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

N

L

P

P

K

K

G

G

V

V

L

V

Y

Y

E

S

H

H

R

R

S

A

N

L

V

V

I

L

H
|
H

A

S

I

L

T

A

E

A

I

S

Q

L

P

V

D

D

V

G

F

T

D

A

L

L

S

S

N

E

R

K

A

D

V

V

L

L

P

P

I

V

V

V

P

P

M

M

F

F

H
|
H

A
x
V

N

N

S

A

W

W

G

C

V

L

P

P

Y

Y

A

A

A

T

T

L

V

V

G

G

A

A

K

K

L

Q

V

V

F

L

S

P

A

G

T

P

N

R

-

L

D

D

A

P

Q

A

V

S

L

L

C

V

D

E

L

L

M

F

H

D

D

G

E

E

G

G

V

V

T

T

H

F

S

T

A

A

G

G

V

V

P

P

T

T

V

V

W

W

I

L

A

A

M

L

F

A

A

D

H

Y

M

L

D

E

A

S

T

T

G

G

I

H

G

R

Y

L

G

K

K

T

L

L

R

R

V

L

I

V

G

G

G
|
G

S
|
S

A
|
A

A

A

P

P

R

R

A

S

M

L

I

I

E

A

R

R

F

F

M

E

R

R

A

M

G

G

I

V

D

E

V

V

G

R

H

Q

A

G

W
x
Y

G
|
G

M
x
L

T
|
T

E
|
E

T

T

S

S

P

P

I

V

G

V

T

V

M

Q

G

N

K

F

R

V

P

K

W

S

N

H

W

L

D

E

D

S

M

L

S

S

F

E

D

E

E

E

Q

K

V

L

D

T

M

L

V

K

C

A

R

K

Q

T

G

G

C

L

P

P

P

I

F

P

G

L

V

V

E

R

L

L

R

R

I

V

V

A

D

D

D

E

E

E

G

G

S

R

E

P

L

V

P

P

R

K

D

D

G

G

R

K

T

A

S

L

G

G

R

E

L

V

Q

Q

I

L

R

K

G

G

P

P

W

W

I

I

I

T

R

G

Y

Y

F

Y

-

G

-

N

-

E

R

E

A

A

G

T

E

R

D

S

A

A

V

L

D

T

E

P

G

D

D

G

W

F

F

F

D

R

T

T

G

G

D
|
D

V

I

S

A

V

V

I

W

H

D

P

E

D

E

G

G

V

Y

M

V

Q

E

I
|
I

T

K

D

D

R

R

A

L

K

K

D

D

V

L

I

I

K
|
K

S

S

G

G

E

E

W
|
W

I

I

S

S

I

V

E

D

L

L

E

E

N

N

A

A

V

V

G

-

A

-

P

-

G

-

V

-

Q

-

E

-

A

-

V

V

G

A

V

I

Y

P

H

H

P

P

K

K

W

W

D

Q

E

E

R

R

P

P

I

L

L

A

L

V

I

V

V

-

K

-

P

-

G

-

A

-

D

-

V

-

G

-

E

-

A

-

D

-

I

-

L

-

D

-

Y

-

L

-

K

-

D

-

K

G

V

F

A

A

K

K

W

W

W

Q

L

L

P

P

D

D

E

-

I

-

V

-

F

-

V

-

D

-

E

-

L

-

P

-

H

-

T

-

A

-

T

-

G

-

K

A

I

Y

L

L

K

K

R

R

Q

A

I

L

R

R

D

E

D

Q

Y

Y

K

K

D

N

Y

Y

active site: T177 (= T177), H197 (= H197), H223 (= H223), T320 (= T319), E321 (= E320), K432 (= K428), W437 (= W433)
binding phosphoaminophosphonic acid-adenylate ester: H223 (= H223), V224 (≠ A224), G295 (= G294), S296 (= S295), A297 (= A296), Y317 (≠ W316), G318 (= G317), L319 (≠ M318), T320 (= T319), D411 (= D407), I423 (= I419), K432 (= K428), W437 (= W433)
binding magnesium ion: T177 (= T177), E321 (= E320)

8i8dA Acyl-acp synthetase structure bound to mc7-acp
33% identity, 96% coverage: 8:520/534 of query aligns to 10:526/529 of 8i8dA

query
sites
8i8dA

L

L

V

I

T

K

S

N

L

L

I

L

D

F

H

S

A

P

A

V

R

A

E

F

H

N

A

P

G

E

R

Q

E

E

I

I

V

V

T

Y

R

-

W

-

A

-

D

-

G

A

S

N

M

H

T

R

R

R

S

H

T

S

W

Y

G

K

E

T

V

F

G

H

T

D

D

R

A

V

R

R

R

Q

F

F

A

A

A

N

A

A

L

L

T

T

K

K

L

M

G

G

M

V

K

K

G

G

D

D

R

T

I

V

A

A

T

V

L

M

A

D

M

Y

N

D

H

S

G

H

H

R

H

Y

L

L

V

E

S

C

W

Y

Y

F

G

A

T

I

A

P

G

M

M

I

G

G

G

A

V

K

L

L

H

H

T

M

V

I

N

N

P

V

R
|
R

L

L

F

S

D

P

E

E

Q

Q

L

I

V

L

Y

Y

I

T

I

I

N

D

H

H

A

A

G

E

D

D

R

D

M

I

L

I

F

L

F

I

D

H

A

E

F

F

L

L

P

P

I

I

V

L

E

D

R

Q

L

I

R

K

D

G

Q

R

L

I

P

D

T

T

V

V

E

T

H

R

F

Y

V

V

L

V

F

L

D

-

A

-

P

-

A

R

R

D

E

D

G

E

Y

E

L

C

S

E

Y

Y

R

E

D

R

L

L

V

L

E

E

A

Q

E

E

D

S

G

T

R

E

F

Y

E

N

W

F

V

P

E

D

L

F

D

D

E

E

N

N

D

T

P

V

V

A

G

T

L

T

C

F

Y

Y

T

T

S

T

G

G

T

T

G

G

N

F

P

P

K

K

G

G

V

V

L

F

Y

F

E

T

H

H

R

R

S

Q

N

L

V

V

I

L

H

H

A

T

-

M

-

G

-

I

I

L

T

S

E

T

I

I

Q

G

P

T

D

N

V

A

F

S

D

Q

-

G

-

R

L

L

S

H

N

Q

R

G

A

D

V

I

V

Y

L

M

P

P

I

I

V

T

P
|
P

M

M

F

F

H
|
H

A

V

N

H

S

A

W
|
W

G

G

V

L

P

P

Y

Y

A

M

A

A

A

T

T

M

V

L

G

G

A

V

K

K

L

Q

V

V

F

Y

S

P

A

G

T

K

N

Y

D

V

A

P

Q

D

V

V

L

L

C

L

D

N

L

L

M

I

H

E

D

Q

E

E

G

K

V

V

T

T

H

F

S

S

A

H

G

C

V

V

P

P

T

T

V
x
I

W

L

I

H

A

L

M

L

F

L

A

S

H

S

M

P

D

K

A

S

T

K

G

A

I

M

G

D

Y

F

G

S

K

G

L

W

R

K

R

-

V

V

I

V

I

I

G
|
G

S

A

A
|
A

A

L

P

P

R

K

A

A

M

L

I

C

E

K

R

S

F

A

M

L

R

E

A

R

G

D

I

I

D

D

V

V

G

F

H

A

A
x
G

W
x
Y

G
|
G

M
|
M

T
x
S

E

E

T

T

S

G

P
|
P

I

I

G

L

T

S

M

I

G

V

K

Q

R

L

P

T

W

P

N

E

W

Q

D

L

D

E

M

L

S

D

F

V

D

D

E

Q

Q

Q

V

A

D

E

M

Y

V

R

C

S

R

K

Q

T

G

G

C

K

P

K

P

V

F

A

G

L

V

V

E

E

L

A

R

Y

I

I

V

V

D

D

D

E

E

D

G

M

S

N

E

K

L

L

P

P

R

H

D

D

G

G

R

E

T

T

S

A

G

G

R

E

L

I

Q

V

I

V

R

R

G

A

P

P

W

W

I

L

I

T

R

P

R

N

Y

Y

F

Y

R

K

A

D

G

N

E

K

D

N

-

S

-

K

A

A

V

L

D

W

E

R

G

G

D

G

W

Y

F

L

D

H

T

T

G

G

D
|
D

V

V

S

A

V

H

I

I

H

D

P

D

D

E

G

G

V

F

M

I

Q

K

I

I

T

T

D

D

R

R

A

V

K

K

D

D

V

M

I

I

K
|
K

S

I

G

S

G
|
G

E

E

W

W

I

V

S

S

I

L

E

E

L

L

E

E

N

D

A

I

A

L

V

H

G

Q

A

H

P

Q

G

S

V

V

Q

S

E

E

A

V

A

A

A

V

V

I

G

G

V

M

Y

P

H

H

P

N

K

K

W

W

D

G

E

E

R

V

P

P

I

L

L

A

L

L

I

V

V

T

K

L

K

K

P

E

G

D

A

A

D

Q

V

V

G

T

E

E

A

K

D

E

I

L

L

L

D

G

Y

F

L

A

K

K

D

D

K

F

V

I

A

N

K

K

W

G

W

I

L

L

P

A

D

R

E

E

-

A

-

L

-

K

I

V

V

K

F

I

V

V

D

D

E

E

L

I

P

A

H

K

T

T

A

S

T

V

G

G

K

K

I

V

L

D

K

K

R

K

Q

E

I

L

R

R

binding adenosine monophosphate: G292 (= G293), G293 (= G294), A295 (= A296), G314 (≠ A315), Y315 (≠ W316), G316 (= G317), M317 (= M318), S318 (≠ T319), D408 (= D407), K429 (= K428)
binding 7-methoxy-7-oxidanylidene-heptanoic acid: H223 (= H223), W227 (= W227), G292 (= G293), G316 (= G317), P322 (= P323)
binding N~3~-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-sulfanylethyl)-beta-alaninamide: R93 (= R95), P220 (= P220), H223 (= H223), I269 (≠ V269), G432 (= G431)

8i6mA Acyl-acp synthetase structure bound to amp-c18:1
33% identity, 96% coverage: 8:520/534 of query aligns to 8:524/528 of 8i6mA

query
sites
8i6mA

L

L

V

I

T

K

S

N

L

L

I

L

D

F

H

S

A

P

A

V

R

A

E

F

H

N

A

P

G

E

R

Q

E

E

I

I

V

V

T

Y

R

-

W

-

A

-

D

-

G

A

S

N

M

H

T

R

R

R

S

H

T

S

W

Y

G

K

E

T

V

F

G

H

T

D

D

R

A

V

R

R

R

Q

F

F

A

A

A

N

A

A

L

L

T

T

K

K

L

M

G

G

M

V

K

K

G

G

D

D

R

T

I

V

A

A

T

V

L

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binding adenosine monophosphate: G291 (= G294), A293 (= A296), G312 (≠ A315), Y313 (≠ W316), G314 (= G317), M315 (= M318), S316 (≠ T319), D406 (= D407), R421 (= R422)
binding magnesium ion: M315 (= M318), S316 (≠ T319), E317 (= E320)

8i51A Acyl-acp synthetase structure bound to amp-mc7
33% identity, 96% coverage: 8:520/534 of query aligns to 8:524/528 of 8i51A

query
sites
8i51A

L

L

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