SitesBLAST
Comparing WP_089216827.1 NCBI__GCF_900188185.1:WP_089216827.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
64% identity, 98% coverage: 2:208/211 of query aligns to 16:225/229 of P77165
- C99 (= C82) binding [2Fe-2S] cluster
- C104 (= C87) binding [2Fe-2S] cluster
- G105 (= G88) binding [2Fe-2S] cluster
- C107 (= C90) binding [2Fe-2S] cluster
- C119 (= C102) binding [2Fe-2S] cluster
- C158 (= C141) binding [2Fe-2S] cluster
- C161 (= C144) binding [2Fe-2S] cluster
- C208 (= C191) binding [2Fe-2S] cluster
- C210 (= C193) binding [2Fe-2S] cluster
5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
71% identity, 82% coverage: 36:209/211 of query aligns to 2:175/175 of 5g5gA
- binding fe2/s2 (inorganic) cluster: G47 (= G81), C48 (= C82), D49 (= D83), G51 (= G85), C53 (= C87), G54 (= G88), C56 (= C90), C68 (= C102), C107 (= C141), G108 (= G142), C110 (= C144), C157 (= C191), C159 (= C193)
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
56% identity, 79% coverage: 45:211/211 of query aligns to 2:156/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G81), C41 (= C82), D42 (= D83), G44 (= G85), C46 (= C87), G47 (= G88), C49 (= C90), C61 (= C102), C101 (= C141), G102 (= G142), C104 (= C144), C136 (= C191), C138 (= C193)
- binding pterin cytosine dinucleotide: Q100 (= Q140), C138 (= C193)
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
43% identity, 79% coverage: 44:209/211 of query aligns to 10:159/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C82), S50 (≠ G85), C52 (= C87), G53 (= G88), C55 (= C90), C67 (= C102), C106 (= C141), G107 (= G142), C109 (= C144), C141 (= C191), C143 (= C193)
- binding pterin cytosine dinucleotide: Q105 (= Q140), C143 (= C193)
1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
42% identity, 78% coverage: 43:207/211 of query aligns to 3:152/162 of 1t3qA
- binding fe2/s2 (inorganic) cluster: I40 (≠ K80), C42 (= C82), E43 (≠ D83), G45 (= G85), C47 (= C87), G48 (= G88), C50 (= C90), R60 (≠ N100), C62 (= C102), C101 (= C141), G102 (= G142), C104 (= C144), C136 (= C191), C138 (= C193)
- binding pterin cytosine dinucleotide: Q100 (= Q140), C138 (= C193)
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K80), C40 (= C82), E41 (≠ D83), G43 (= G85), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K80), C40 (= C82), E41 (≠ D83), G43 (= G85), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K80), C40 (= C82), E41 (≠ D83), G43 (= G85), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of 4c7yA
- binding fe2/s2 (inorganic) cluster: C40 (= C82), E41 (≠ D83), G43 (= G85), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 535, 539
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
- binding hydrogen peroxide: 696, 697, 869
3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of 3fc4A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K80), C40 (= C82), E41 (≠ D83), G43 (= G85), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 1,2-ethanediol: 535, 622, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of 3fahA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K80), C40 (= C82), E41 (≠ D83), G43 (= G85), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of 1sijA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K80), C40 (= C82), E41 (≠ D83), G43 (= G85), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), Q99 (= Q140), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding arsenite: 535, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Q46509 Aldehyde oxidoreductase; Molybdenum iron sulfur protein; EC 1.2.99.7 from Megalodesulfovibrio gigas (Desulfovibrio gigas) (see paper)
38% identity, 78% coverage: 43:207/211 of query aligns to 1:153/907 of Q46509
- C40 (= C82) binding [2Fe-2S] cluster
- C45 (= C87) binding [2Fe-2S] cluster
- C48 (= C90) binding [2Fe-2S] cluster
- C60 (= C102) binding [2Fe-2S] cluster
- C100 (= C141) binding [2Fe-2S] cluster
- C103 (= C144) binding [2Fe-2S] cluster
- C137 (= C191) binding [2Fe-2S] cluster
- C139 (= C193) binding [2Fe-2S] cluster
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
44% identity, 77% coverage: 46:207/211 of query aligns to 5:151/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C82), S44 (≠ G85), H45 (≠ Q86), C46 (= C87), G47 (= G88), C49 (= C90), C61 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C135 (= C191), C137 (= C193)
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
44% identity, 77% coverage: 46:207/211 of query aligns to 4:150/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ K80), C40 (= C82), S43 (≠ G85), C45 (= C87), G46 (= G88), C48 (= C90), C60 (= C102), C99 (= C141), G100 (= G142), C102 (= C144), C134 (= C191), C136 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q140), C136 (= C193)
1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
42% identity, 78% coverage: 43:207/211 of query aligns to 1:153/906 of 1dgjA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K80), G39 (= G81), C40 (= C82), G41 (≠ D83), G43 (= G85), Q44 (= Q86), C45 (= C87), G46 (= G88), C48 (= C90), R58 (≠ N100), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C137 (= C191), C139 (= C193)
- binding pterin cytosine dinucleotide: Q99 (= Q140), C139 (= C193)
Sites not aligning to the query:
- active site: 391, 427, 503, 507, 535, 869, 870
- binding molybdenum (iv)oxide: 424, 535, 698, 869
- binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869
P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
43% identity, 79% coverage: 44:209/211 of query aligns to 4:155/166 of P19921
- C42 (= C82) binding [2Fe-2S] cluster
- C47 (= C87) binding [2Fe-2S] cluster
- C50 (= C90) binding [2Fe-2S] cluster
- C62 (= C102) binding [2Fe-2S] cluster
- C102 (= C141) binding [2Fe-2S] cluster
- C105 (= C144) binding [2Fe-2S] cluster
- C137 (= C191) binding [2Fe-2S] cluster
- C139 (= C193) binding [2Fe-2S] cluster
1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
43% identity, 79% coverage: 44:209/211 of query aligns to 2:153/161 of 1n5wA
- binding flavin-adenine dinucleotide: S43 (≠ G85), H44 (≠ Q86)
- binding fe2/s2 (inorganic) cluster: I38 (≠ K80), G39 (= G81), C40 (= C82), S43 (≠ G85), C45 (= C87), G46 (= G88), C48 (= C90), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C135 (= C191), C137 (= C193)
1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
43% identity, 79% coverage: 44:209/211 of query aligns to 2:153/158 of 1n5wD
- binding flavin-adenine dinucleotide: S43 (≠ G85), H44 (≠ Q86)
- binding fe2/s2 (inorganic) cluster: C40 (= C82), S43 (≠ G85), C45 (= C87), G46 (= G88), C48 (= C90), C60 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C135 (= C191), C137 (= C193)
- binding pterin cytosine dinucleotide: Q99 (= Q140), C137 (= C193)
1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
41% identity, 77% coverage: 46:207/211 of query aligns to 5:151/161 of 1sb3C
- binding fe2/s2 (inorganic) cluster: Q39 (≠ K80), C41 (= C82), G44 (= G85), C46 (= C87), G47 (= G88), C49 (= C90), C61 (= C102), C100 (= C141), G101 (= G142), C103 (= C144), C135 (= C191), C137 (= C193)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q140), C137 (= C193)
Query Sequence
>WP_089216827.1 NCBI__GCF_900188185.1:WP_089216827.1
MQSPGDFEMSRRGMLAASAASAAVTTIPAARAKTASAQPAPSMARIALTVNGEARELELD
TRTTLLDALREHLQLTGTKKGCDHGQCGACTVIVDGRRINSCLSLAVMHDGDTVTTIEGL
GTPDDLHPMQAAFIVHDGYQCGYCTPGQICSAVAVLEEIEAGIPSHVTGDLTAPAEATNM
EIRERMSGNICRCGAYSNILEAITEVAGRQA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory