SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_089217430.1 NCBI__GCF_900188185.1:WP_089217430.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q91WL8 WW domain-containing oxidoreductase; EC 1.1.1.- from Mus musculus (Mouse) (see 3 papers)
34% identity, 91% coverage: 14:292/306 of query aligns to 121:401/414 of Q91WL8

query
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Q91WL8

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Sites not aligning to the query:

33 modified: Phosphotyrosine
51:54 KRKR→QGTG: Abolishes the TNF-induced nuclear translocation.
125:414 Interaction with MAPT

Q9NZC7 WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase; Short chain dehydrogenase/reductase family 41C member 1; EC 1.1.1.- from Homo sapiens (Human) (see 8 papers)
33% identity, 91% coverage: 14:292/306 of query aligns to 121:401/414 of Q9NZC7

query
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Q9NZC7

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A179 (≠ F72) to T: in dbSNP:rs11545029
L272 (vs. gap) to F: in dbSNP:rs186745328
P282 (≠ A167) to A: in dbSNP:rs3764340
Y287 (≠ N172) modified: Phosphotyrosine; by TNK2; mutation to A: Loss of phosphorylation by TNK2.
L291 (≠ S175) to P: found in a esophageal cancer sample; somatic mutation; dbSNP:rs119487098
R314 (≠ M200) to H: in dbSNP:rs73572838

Sites not aligning to the query:

28 K→T: No effect on interaction with TP53. Abolishes interaction with MAPK8; when associated with V-29.
29 D→V: No effect on interaction with TP53. Abolishes interaction with MAPK8; when associated with T-28.
33 modified: Phosphotyrosine; Y→F: Loss of phosphorylation.; Y→R: Abolishes interaction with TP53, TP73, MAPK8 and ERBB4. Partial loss of interaction with TFAP2C. Loss of phosphorylation. Loss of the proapoptotic activity. Abolishes binding to VOPP1.
44:47 WEHP→FEHA: Abolishes interaction with LITAF.
61 Y→R: No effect on interaction with TP73.
85:88 YLDP→ALDA: No effect on interaction with LITAF.
98 P → L: in dbSNP:rs144601717
111 T → S: in a Burkitt lymphoma cell line; dbSNP:rs114755364
120 R → W: in a primary colorectal tumor and a histiocytic lymphoma cell line; dbSNP:rs141361080

5o98B Binary complex of catharanthus roseus vitrosamine synthase with NADP+ (see paper)
29% identity, 66% coverage: 16:218/306 of query aligns to 1:244/283 of 5o98B

query
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5o98B

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G24), N11 (= N26), K12 (≠ S27), G13 (= G28), I14 (≠ V29), R34 (= R49), R38 (≠ K53), D61 (= D76), V62 (≠ L77), N88 (= N102), A89 (= A103), G90 (= G104), I122 (≠ V123), I149 (≠ T150), S150 (= S151), Y207 (≠ N184), K211 (≠ M188), P235 (= P209), G236 (= G210), F237 (= F211), A238 (= A212), T240 (= T214), L242 (≠ F216), N243 (≠ G217)

7jk9A filaments of plant light-dependent protochlorophyllide oxidoreductase (LPOR) bound to NADPH, Pchlide, and membrane (see paper)
28% identity, 91% coverage: 20:298/306 of query aligns to 6:315/316 of 7jk9A

query
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7jk9A

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R

L

F

Q

H

A

-

A

E

E

E

M

T

P

G

V

V

T

T

T

F

V

A

G

S

C

L

H
x
Y

P

P

G
|
G

-
x
C

F
x
I

A

A

R

S

T
|
T

N
x
G

F
x
L

G
x
F

R
|
R

A

E

S

H

L

I

A

P

G

L

R
x
F

L

R

A

A

V

L

G
x
F

-

P

-

F

-

Q

-

K

-

Y

-

I

V

T

G

K

G

G

L

Y

L

V

F

S

N

E

T

T

P

E

A

S

M

G

G

K

A

R

W

L

P

A

T

Q

L

V

Q

V

A

S

A

D

T

P

G

S

N

L

V

T

K

K

P

S

G

G

G

V

Y

Y

Y

W

G

S

P

W

T

N

A

N

F

A

A

S

E

-

L

-

R

-

G

-

P

A

S

S

F

F

E

E

C

N

T

Q

P

L

S

S

D

E

A

A

R

S

N

D

P

V

Q

E

L

K

A

A

R

R

R

K

L

V

W

W

D

E

V

I

S

S

I

E

E

K

M

L

T

V

G

G

I

L

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G24), S12 (≠ N26), S13 (= S27), L15 (≠ V29), R36 (= R49), K40 (= K53), L60 (= L75), D61 (= D76), L62 (= L77), N88 (= N102), A89 (= A103), A90 (≠ G104), V91 (= V105), V141 (≠ T150), Y191 (= Y177), G223 (= G210), I225 (≠ F211), T228 (= T214), G229 (≠ N215), L230 (≠ F216), R232 (= R218)
binding Protochlorophyllide: Y92 (≠ T106), L151 (vs. gap), A152 (vs. gap), Y191 (= Y177), Y221 (≠ H208), C224 (vs. gap), L230 (≠ F216), F231 (≠ G217), F238 (≠ R224), F242 (≠ G228)

6r46A Crystal structure of lpor (thermosynechococcus elongatus) complexed with NADP+ at 2.5a resolution (see paper)
30% identity, 91% coverage: 20:298/306 of query aligns to 5:274/275 of 6r46A

query
sites
6r46A

L

V

I

I

V

I

T

T

G
|
G

A

A

N
x
S

S
|
S

G
|
G

V
|
V

G

G

F

L

E

Y

V

A

A

T

S

K

A

A

L

L

A

A

A

N

R

R

R

G

A

W

R

H

V

V

L

I

L

M

A

A

C
|
C

R
|
R

D

N

E

L

S

E

K

K

A

A

R

E

A

Q

A

A

M

A

D

K

K

N

I

L

R

Q

Q

I

R

P

S

P

S

E

G

A

A

Y

D

T

L

I

A

-

F

-

L

L

P

H

L
|
L

D
|
D

L

L

A

S

D

S

L

L

A

A

S

S

V

V

R

R

K

G

A

F

A

V

E

E

V

S

-

F

-

R

A

A

A

L

K

N

E

R

P

P

R

-

I

L

D

R

V

A

L

L

I

V

N

C

N
|
N

A

A

G
x
A

V
|
V

T

Y

V

Y

P

P

T

L

R

L

Q

K

T

E

-

P

-

I

-

Y

T

S

R

V

Q

D

G

G

F

Y

E

E

L

I

L

T

F

V

G

A

V

T

N

N

H

H

L

L

G

G

S

H

F

F

A

L

L

L

T

I

A

N

L

L

M

L

L

L

P

E

K

D

L

L

R

K

E

N

T

S

P

P

G

E

S

S

-

D

-

K

R

R

I

L

V

V

I

I

T

L

S

G

S

T

-

V

-

T

-

A

-

N

-

P

-

D

-

L

-

G

-

N

-

L

-

E

G

G

Q

F

H

E

K

K

G

G

A

F

K

K

I

K

E

P

W

I

D

A

D

M

L

I

N

N

A

G

E

-

K

K

N

P

Y

F

N

K

W

S

L

G

S

K

R

A

Y

F

G

K

A

D

S

S

K
|
K

L

L

A

C

N

N

L

M

L

L

F

T

M

A

L

R

E

E

L

L

N

H

R

R

L

F

Q

H

A

E

A

S

E

T

M

G

P

I

V

V

T

F

T

N

V

-

G

S

C

L

H

Y

P

P

G

G

F

C

A

V

R

A

T

D

N

T

F

Y

G

V

R

S

A

Q

S

E

L

L

A

A

G

G

-

E

R

R

L

V

A

A

V

M

G

V

V

V

G

A

G

D

L

P

L

E

F

F

N

R

T

Q

P

S

A

G

M

V

G

H

-

W

A

S

W

W

P

-

T

-

L

-

Q

-

A

-

T

-

G

-

N

-

V

-

K

-

P

-

G

-

Y

-

G

-

P

-

T

-

A

-

F

-

A

-

E

-

L

-

R

-

G

G

P

K

S

A

F

F

E

V

C

Q

T

E

P

L

S

S

D

A

D

E

A

A

R

S

N

D

P

E

Q

Q

L

K

A

A

R

R

L

L

W

W

D

E

V

L

S

S

I

E

E

K

M

L

T

V

G

G

I

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G24), S11 (≠ N26), S12 (= S27), G13 (= G28), V14 (= V29), C33 (= C48), R34 (= R49), L58 (= L75), D59 (= D76), N86 (= N102), A88 (≠ G104), V89 (= V105), K181 (= K181)

6l1hB Crystal structure of light-dependent protochlorophyllide oxidoreductase from thermosynechococcus elongatus (see paper)
29% identity, 91% coverage: 20:298/306 of query aligns to 5:305/305 of 6l1hB

query
sites
6l1hB

L

V

I

I

V

I

T

T

G
|
G

A

A

N
x
S

S
|
S

G
|
G

V

V

G

G

F

L

E

Y

V

A

A

T

S

K

A

A

L

L

A

A

A

N

R

R

R

G

A

W

R

H

V

V

L

I

L

M

A

A

C

C

R
|
R

D

N

E

L

S

E

K
|
K

A

A

R

E

A

Q

A

A

M

A

D

K

K

N

I

L

R

Q

Q

I

R

P

S

P

S

E

G

A

A

Y

D

T

L

I

A

-

F

-

L

L

P

H

L
|
L

D
|
D

L
|
L

A

S

D

S

L

L

A

A

S

S

V

V

R

R

K

G

A

F

A

V

E

E

V

S

-

F

-

R

A

A

A

L

K

N

E

R

P

P

R

-

I

L

D

R

V

A

L

L

I

V

N

C

N
|
N

A
|
A

G
x
A

V
|
V

T

Y

V

Y

P

P

T

L

R

L

Q

K

T

E

-

P

-

I

-

Y

T

S

R

V

Q

D

G

G

F

Y

E

E

L

I

L

T

F

V

G

A

V
x
T

N

N

H

H

L

L

G

G

S

H

F

F

A

L

L

L

T

I

A

N

L

L

M

L

L

L

P

E

K

D

L

L

R

K

E

N

T

S

P

P

G

E

S

S

-

D

-

K

R

R

I

L

V

V

I

I

T

L

S
x
G

S

T

-

V

-

T

-

A

-

N

-

R

-

P

-

D

-

L

-

G

-

N

-

L

-

E

G

G

Q

F

H

E

K

K

G

G

A

F

K

K

I

K

E

P

W

I

D

A

D

M

L

I

N

N

A

G

E

-

K

K

N

P

Y

F

N

K

W

S

L

G

S

K

R

A

Y
|
Y

G

K

A

D

S

S

K
|
K

L

L

A

C

N

N

L

M

L

L

F

T

M

A

L

R

E

E

L

L

N

H

R

R

L

F

Q

H

A

E

A

S

E

T

M

G

P

I

V

V

T

F

T

N

V

-

G

S

C

L

H
x
Y

P

P

G

G

-

C

-
x
V

-

A

-

D

-
x
T

-
x
P

F
x
L

A

F

R
|
R

T

H

N

H

F

F

G

P

R

L

-

F

A

Q

S

K

L

L

A

F

G

P

R

L

L

F

A

Q

V

K

G

K

V

I

G

T

G

G

L

G

L

Y

F

V

N

S

T

Q

P

E

A

L

M

A

G

G

A

E

W

R

P

V

T

A

L

M

Q

V

A

V

A

A

T

D

G

P

N

E

V

F

K

R

P

Q

G

S

G

G

Y

V

Y

H

G

W

P

S

T

W

A

G

F

N

A

R

E

Q

L

K

R

E

G

G

-

R

P

K

S

A

F

F

E

V

C

Q

T

E

P

L

S

S

D

A

D

E

A

A

R

S

N

D

P

E

Q

Q

L

K

A

A

R

R

L

L

W

W

D

E

V

L

S

S

I

E

E

K

M

L

T

V

G

G

I

L

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G24), S11 (≠ N26), S12 (= S27), G13 (= G28), R34 (= R49), K38 (= K53), L58 (= L75), D59 (= D76), L60 (= L77), N86 (= N102), A87 (= A103), A88 (≠ G104), V89 (= V105), T110 (≠ V123), G140 (≠ S151), Y177 (= Y177), K181 (= K181), Y207 (≠ H208), V211 (vs. gap), T214 (vs. gap), P215 (vs. gap), L216 (≠ F211), R218 (= R213)

5t2uA Short chain dehydrogenase/reductase family protein (see paper)
34% identity, 65% coverage: 17:214/306 of query aligns to 6:183/241 of 5t2uA

query
sites
5t2uA

G

G

K

L

C

T

L

A

I

L

V

V

T

T

G
|
G

A

G

N

T

S

S

G
|
G

V

I

G

G

F

L

E

E

V

S

A

A

S

R

A

L

L

M

A

A

R

E

R

G

A

A

R

D

V

V

L

V

L

I

A

T

C

G

R
|
R

D
|
D

E

A

S

Q

K
x
R

A

G

R

E

A

Q

A

A

M

A

D

A

K

D

I

I

R

-

Q

-

R

-

S

-

G

G

A

H

D

G

L

A

A

R

F

F

L

V

P

Q

L

A

D
|
D

L
|
L

A

G

D

D

L

L

A

D

S

S

V

V

R

-

K

-

A

-

A

A

E

D

V

L

A

A

K

Q

E

A

P

P

R

D

I

V

D

D

V

I

L

L

I

V

N

N

N
|
N

A
|
A

G

G

V

I

T

-

V
x
Y

P

P

T

Q

R

A

Q

S

T

T

T

F

R

D

Q

Q

-

D

-

V

-

A

G

G

F

F

E

Q

L

Q

L

L

F

F

G

D

V

T

N

N

H

V

L

R

G

G

S

T

F

Y

A

F

L

L

T

V

A

A

L

A

M

A

L

A

P

K

K

G

L

M

-

V

R

A

E

R

T

G

P

H

G

G

S

S

R

I

I

V

V

N

I
|
I

T

T

S
x
T

S

L

G

A

Q

A

H

H

K

K

G

G

A

-

K

-

I

-

E

-

W

-

D

-

L

-

N

-

A

-

E

-

K

-

N

-

Y

F

N

P

W

G

L
x
T

S

S

R

V

Y
|
Y

G

G

A

A

S

T

K
|
K

L

A

A

A

N

-

L

-

F

-

M

-

L

L

E

E

L

S

N

L

R

T

R

R

L

T

Q

W

A

A

E

E

M

F

P

G

V

A

T

N

T

G

V

V

-

R

-

V

-

N

G

S

C

V

H

S

P
|
P

G

G

F
x
P

A
x
T

R

R

T
|
T

active site: G17 (= G28), T135 (≠ S151), T145 (≠ L174), Y148 (= Y177), K152 (= K181)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G24), G17 (= G28), R38 (= R49), D39 (= D50), R42 (≠ K53), D60 (= D76), L61 (= L77), N83 (= N102), A84 (= A103), Y87 (≠ V107), I133 (= I149), T135 (≠ S151), Y148 (= Y177), K152 (= K181), P178 (= P209), P180 (≠ F211), T181 (≠ A212), T183 (= T214)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 185, 186

4nbuB Crystal structure of fabg from bacillus sp (see paper)
33% identity, 44% coverage: 18:152/306 of query aligns to 8:139/244 of 4nbuB

query
sites
4nbuB

K

K

C

V

L

A

I

I

V

I

T

T

G
|
G

A

A

N

A

S
x
N

G
|
G

V
x
I

G

G

F

L

E

E

V

A

A

A

S

R

A

V

L

F

A

M

A

K

R

E

R

G

A

A

R

K

V

V

L

V

L

I

A

A

C
x
D

R
x
F

D

N

E

E

S

A

K

A

A

G

R

K

A

E

A

A

M

V

D

E

K

-

I

-

R

-

Q

-

R

-

S

-

S

A

G

N

A

P

D

G

L

V

A

V

F

F

L

I

P

R

L
x
V

D
|
D

L
x
V

A

S

D

D

L

R

A

E

S

S

V

V

R

H

K

R

A

L

A

V

E

E

-

N

V

V

A

A

A

E

K

R

E

F

P

G

R

K

I

I

D

D

V

I

L

L

I

I

N

N

N
|
N

A
|
A

G
|
G

V
x
I

T

T

-

R

-
x
D

-

S

V

M

P

L

T

S

R
x
K

Q

M

T

T

T

V

R

D

Q

Q

G

-

F

F

E

Q

L

Q

L

V

F

I

G

N

V

V

N
|
N

H

L

L

T

G

G

S

V

F

F

A

H

L

C

T

T

A

Q

L

A

M

V

L

L

P

P

K

Y

L

M

R

A

E

E

T

Q

P

G

G

K

S

G

R

K

I

I

V

I

I

N

T
|
T

S

S

S
|
S

active site: G18 (= G28), N111 (= N124), S139 (= S152)
binding acetoacetyl-coenzyme a: D93 (vs. gap), K98 (≠ R110), S139 (= S152)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G24), N17 (≠ S27), G18 (= G28), I19 (≠ V29), D38 (≠ C48), F39 (≠ R49), V59 (≠ L75), D60 (= D76), V61 (≠ L77), N87 (= N102), A88 (= A103), G89 (= G104), I90 (≠ V105), T137 (= T150), S139 (= S152)

Sites not aligning to the query:

active site: 149, 152, 156
binding acetoacetyl-coenzyme a: 146, 147, 149, 152, 184, 189, 200
binding 1,4-dihydronicotinamide adenine dinucleotide: 152, 156, 182, 184, 185, 187, 189

3o26A The structure of salutaridine reductase from papaver somniferum. (see paper)
36% identity, 29% coverage: 18:105/306 of query aligns to 2:90/294 of 3o26A

query
sites
3o26A

K

R

C

C

L

A

I

V

V

V

T

T

G
|
G

A

G

N
|
N

S
x
K

G
|
G

V
x
I

G

G

F

F

E

E

V

I

A

C

S

K

A

Q

L

L

A

S

R

N

R

G

A

I

R

M

V

V

L

V

L

L

A

T

C

C

R
|
R

D

D

E

V

S

T

K

K

A

G

R

H

A

E

A

A

M

V

D

E

K

K

I

L

R

K

Q

N

R

-

S

S

S

N

G

H

A

E

D

N

L

V

A

V

F

F

L

H

P

Q

L

L

D
|
D

L
x
V

A

T

D

D

-

P

L

I

A

A

S

T

V

M

R

S

K

S

A

L

A

A

E

D

-

F

V

I

A

K

A

T

K

H

E

F

P

G

R

K

I

L

D

D

V

I

L

L

I

V

N

N

N
|
N

A
|
A

G
|
G

V

V

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), N10 (= N26), K11 (≠ S27), G12 (= G28), I13 (≠ V29), R33 (= R49), D59 (= D76), V60 (≠ L77), N87 (= N102), A88 (= A103), G89 (= G104)

Sites not aligning to the query:

active site: 129, 142, 144, 185
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 140, 167, 168, 225, 229, 253, 255, 256, 258, 260, 261

Q071N0 Salutaridine reductase; EC 1.1.1.248 from Papaver somniferum (Opium poppy) (see 2 papers)
36% identity, 29% coverage: 18:105/306 of query aligns to 13:101/311 of Q071N0

query
sites
Q071N0

K

R

C

C

L

A

I

V

V

V

T

T

G

G

A

G

N
|
N

S
x
K

G
|
G

V
x
I

G

G

F

F

E

E

V

I

A

C

S

K

A

Q

L

L

A

S

R

N

R

G

A

I

R

M

V

V

L

V

L

L

A

T

C

C

R
|
R

D

D

E

V

S

T

K

K

A

G

R

H

A

E

A

A

M

V

D

E

K

K

I

L

R

K

Q

N

R

-

S

S

S

N

G

H

A

E

D

N

L

V

A

V

F

F

L

H

P

Q

L

L

D
|
D

L
x
V

A

T

D

D

-

P

L

I

A

A

S

T

V

M

R

S

K

S

A

L

A

A

E

D

-

F

V

I

A

K

A

T

K

H

E

F

P

G

R

K

I

L

D

D

V

I

L

L

I

V

N

N

N
|
N

A

A

G

G

V

V

NKGI 21:24 (≠ NSGV 26:29) binding NADP(+)
R44 (= R49) binding NADP(+)
DV 70:71 (≠ DL 76:77) binding NADP(+)
N98 (= N102) binding NADP(+)

Sites not aligning to the query:

104 F→A: Weak substrate inhibition, decreased substrate affinity and increased reaction velocity. Loss of substrate inhibition and doubled maximal velocity; when associated with A-275.
106 V→A: Decreased substrate affinity.
107 D→A: Decreased substrate affinity.
129 binding substrate
180 binding substrate
181 S→A: No effect on activity.
182 T→A: Slightly decreased affinity, but strongly reduced activity.
185 L→A: Very low activity.; L→S: Increased substrate affinity, but low activity.; L→V: Reduced activity.
186 K→V: No effect on activity.
236 active site, Proton acceptor; binding NADP(+)
240 binding NADP(+)
263 modified: Disulfide link with 305
266 L→A: Decreased substrate affinity.; L→V: No effect on substrate affinity.
267:272 binding NADP(+)
271 M→A: Abrogate substrate inhibition. Decreased substrate affinity and decreased reductase activity.
272 N→A: Abrogate substrate inhibition. Decreased substrate affinity and decreased reductase activity.
275 I→A: Weak substrate inhibition, decreased substrate affinity and increased reaction velocity. Loss of substrate inhibition and doubled maximal velocity; when associated with A-104.; I→V: No effect on substrate affinity.
305 modified: Disulfide link with 263

A4UHT7 Salutaridine reductase; EC 1.1.1.248 from Papaver bracteatum (Great scarlet poppy) (see 2 papers)
26% identity, 44% coverage: 18:152/306 of query aligns to 13:180/311 of A4UHT7

query
sites
A4UHT7

K

R

C

C

L

A

I

V

V

V

T

T

G

G

A

G

N

N

S

K

G

G

V

I

G

G

F

F

E

E

V

I

A

C

S

K

A

Q

L

L

A

S

R

S

R

G

A

I

R

M

V

V

L

V

L

L

A

T

C

C

R
|
R

D

D

E

V

S

T

K
x
R

A

G

R

L

A

E

A

A

M

V

D

E

K

K

I

L

R

K

Q

N

R

-

S

S

S

N

G

H

A

E

D

N

L

V

A

V

F

F

L

H

P

Q

L

L

D

D

L

V

A

T

D

D

-

P

L

I

A

T

S

T

V

M

R

S

K

S

A

L

A

A

E

D

-

F

V

I

A

K

A

A

K

R

E

F

P

G

R

K

I

L

D

D

V

I

L

L

I

V

N

N

A

A

G

G

V

V

-

A

-

G

-
x
F

-

S

-
x
V

-
x
D

-

A

-

D

-

R

-

F

-

K

-

A

-

M

-

I

-

S

-

D

-

I

-

G

-

E

-

D

-

S

-

E

-

V

-

K

-

I

-

Y

-

E

T

K

V

P

P

E

T

A

R

Q

Q

E

T

L

T

M

R

S

Q

E

G

T

F

Y

E

E

L

L

-

A

-

E

L

C

F

L

G

K

V

I

N
|
N

H

Y

L

Y

G

G

S

V

F

K

A

S

L

V

T

T

A

E

L

V

M

L

L

L

P

P

K

L

L

L

R

Q

E

L

T

S

P

D

G

S

S

P

R

R

I

I

V

V

I

N

T

V

S

S

S
|
S

R44 (= R49) mutation to E: More than 50-fold decrease in affinity towards NADPH.
R48 (≠ K53) mutation to E: 30-fold decrease in affinity towards NADPH. 1.5-fold increase in affinity towards NADH.; mutation to K: 2.5-fold decrease in affinity towards NADPH. 2-fold increase in affinity towards NADH.
F104 (vs. gap) mutation to A: Strong decrease in substrate affinity and catalytic efficiency. Strongly reduced substrate inhibition. Almost 200-fold decrease in substrate affinity and 112-fold decrease in catalytic efficiency but no substrate inhibition; when associated with A-275.
V106 (vs. gap) mutation to A: Decrease in substrate affinity. Increase in catalytic efficiency.
D107 (vs. gap) mutation to A: 7-fold decrease in substrate affinity. 15-fold decrease in catalytic efficiency. Strongly reduced substrate inhibition.
N152 (= N124) mutation to A: 6-fold decrease in catalytic efficiency.
S180 (= S152) mutation to A: 3-fold decrease in substrate affinity. More than 2300-fold decrease in catalytic efficiency.

Sites not aligning to the query:

181 S→A: More than 2-fold decrease in substrate affinity. 1.5-fold decrease in catalytic efficiency.
182 T→A: 2-fold decrease in substrate affinity. 20-fold decrease in catalytic efficiency.
185 L→A: 6-fold decrease in substrate affinity. 550-fold decrease in catalytic efficiency. No substrate inhibition.; L→S: Almost 2-fold increase in substrate affinity. Almost 5-fold decrease in catalytic efficiency.; L→V: Almost 2-fold decrease in catalytic efficiency.
186 K→V: Almost 4-fold decrease in substrate affinity. More than 4-fold decrease in catalytic efficiency.
236 Y→F: Inactive.
240 K→E: Inactive.
266 L→A: Strong decrease in substrate affinity and catalytic efficiency. No substrate inhibition.; L→S: 12-fold decrease in substrate affinity and 26-fold decrease in catalytic efficiency.; L→V: 6-fold decrease in substrate affinity. 7-fold decrease in catalytic efficiency.
271 M→A: Almost 11-fold decrease in substrate affinity and 2200-fold decrease in catalytic efficiency but no substrate inhibition.; M→T: Inactive.
272 N→A: Almost 43-fold decrease in substrate affinity and 3300-fold decrease in catalytic efficiency but no substrate inhibition.; N→T: Inactive.
275 I→A: 15-fold decrease in substrate affinity. Almost 7-fold decrease in catalytic efficiency and strongly reduced substrate inhibition. Almost 200-fold decrease in substrate affinity and 112-fold decrease in catalytic efficiency but no substrate inhibition; when associated with A-104.; I→V: Slight decrease in catalytic efficiency.

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
31% identity, 66% coverage: 18:218/306 of query aligns to 9:194/247 of 7tzpG

query
sites
7tzpG

K

K

C

T

L

A

I

I

V

V

T

T

G
|
G

A

A

N

A

S
x
R

G
|
G

V
x
I

G

G

F

F

E

G

V

I

A

A

S

Q

A

V

L

L

A

A

A

R

R

E

R

G

A

A

R

R

V

V

L

I

A

A

C
x
D

R
|
R

D

D

E

A

S

H

K

G

A

E

R

A

A

A

M

A

D

S

K

-

I

-

R

-

Q

L

R

R

S

E

S

S

G

G

A

A

D

Q

L

A

A

L

F

F

L

I

P

S

L
x
C

D

N

L
x
I

A

A

D

E

L

K

A

T

S

Q

V

V

R

-

K

E

A

A

A

L

E

F

V

S

A

Q

A

A

K

E

E

E

P

A

R

F

-

G

-

P

I

V

D

D

V

I

L

L

I

V

N

N

N
|
N

A

A

G
|
G

V
x
I

T

N

-

R

-

D

V

A

P

M

T

L

R

H

Q

K

T

L

T

T

R

E

Q

A

G

D

F

W

E

D

L

T

L

V

F

I

G

D

V
|
V

N

N

H

L

L

K

G

G

S

T

F

F

A

L

L

C

T

M

A

Q

L

Q

M

A

L

A

P

I

K

R

L

M

R

R

E

E

T

R

P

G

G

A

S

G

R

R

I

I

V

I

I

N

T

I

S

A

S

S

G

A

Q

S

H

-

K

-

G

-

A

-

K

-

I

-

E

-

W

W

D

L

D

G

L

N

N

V

A

G

E

Q

K

T

N

N

Y
|
Y

N

S

W

-

L

-

S

-

R

-

Y

-

G

-

A

A

S

S

K
|
K

L

-

A

A

N

G

L

V

F

G

M

M

L

T

E

K

L

T

N

A

R

C

R

R

L

-

Q

E

A

L

A

A

E

K

M

K

P

G

V

V

T

T

T

V

V

N

G

A

C

I

H

C

P

P

G

G

F

F

A
x
I

R

D

T
|
T

N

D

F

M

G
x
T

R

R

binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G24), R18 (≠ S27), G19 (= G28), I20 (≠ V29), D39 (≠ C48), R40 (= R49), C63 (≠ L75), I65 (≠ L77), N91 (= N102), G93 (= G104), I94 (≠ V105), V114 (= V123), Y155 (= Y171), K159 (= K181), I188 (≠ A212), T190 (= T214), T193 (≠ G217)

6gtuA 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with fragment j6
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/268 of 6gtuA

query
sites
6gtuA

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L

V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A
|
A

G
|
G

V

H

T

H

V

P

P

P

T

P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), N86 (= N102), A87 (= A103), G88 (= G104), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 151, 155
binding N-(1,3-benzodioxol-5-ylmethyl)cyclopentanamine: 183, 253
binding nicotinamide-adenine-dinucleotide: 13, 16, 17, 18, 151, 155, 182, 184, 186, 188

6emmA 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with salicylic acid
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/268 of 6emmA

query
sites
6emmA

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L

V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A
|
A

G
|
G

V

H

T

H

V

P

P

P

T

P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), N86 (= N102), A87 (= A103), G88 (= G104), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 151, 155
binding nicotinamide-adenine-dinucleotide: 13, 16, 17, 18, 151, 155, 181, 182, 184, 186, 188

5hs6A Human 17beta-hydroxysteroid dehydrogenase type 14 in complex with estrone (see paper)
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/257 of 5hs6A

query
sites
5hs6A

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L

V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A

A

G

G

V

H

T
x
H

V

P

P

P

T

P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding (9beta,13alpha)-3-hydroxyestra-1,3,5(10)-trien-17-one: H90 (≠ T106)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), N86 (= N102), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 148, 151, 155
binding (9beta,13alpha)-3-hydroxyestra-1,3,5(10)-trien-17-one: 145, 151, 192
binding nicotinamide-adenine-dinucleotide: 13, 16, 17, 18, 151, 155, 181, 182, 183, 184, 186, 188

5o6oA 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with a non-steroidal 2,6-pyridinketone inhibitor (see paper)
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/256 of 5o6oA

query
sites
5o6oA

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L
x
V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A

A

G

G

V

H

T
x
H

V

P

P

P

T

P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding 2-fluoranyl-3-[6-(4-fluoranyl-3-oxidanyl-phenoxy)pyridin-2-yl]phenol: H90 (≠ T106), S138 (= S151)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), V60 (≠ L77), N86 (= N102), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 151, 155
binding 2-fluoranyl-3-[6-(4-fluoranyl-3-oxidanyl-phenoxy)pyridin-2-yl]phenol: 145, 146, 147, 151, 183, 189, 192
binding beta-D-glucopyranose: 185, 186, 187, 190
binding nicotinamide-adenine-dinucleotide: 13, 16, 17, 18, 151, 155, 181, 182, 183, 184, 186, 188

5o42A 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with a non-steroidal 2,6-pyridinketone inhibitor. (see paper)
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/256 of 5o42A

query
sites
5o42A

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L
x
V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A

A

G
|
G

V

H

T
x
H

V

P

P

P

T
x
P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding 2-fluoranyl-3-[6-[1-(4-fluoranyl-3-oxidanyl-phenyl)ethenyl]pyridin-2-yl]phenol: H90 (≠ T106), P93 (≠ T109), S138 (= S151)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), V60 (≠ L77), N86 (= N102), G88 (= G104), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 151, 155
binding 2-fluoranyl-3-[6-[1-(4-fluoranyl-3-oxidanyl-phenyl)ethenyl]pyridin-2-yl]phenol: 145, 146, 151, 183, 189, 192
binding beta-D-glucopyranose: 17, 185, 186, 187, 190
binding nicotinamide-adenine-dinucleotide: 13, 16, 18, 151, 155, 181, 182, 183, 184, 186, 188

5l7wA 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with a non-steroidal inhibitor. (see paper)
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/254 of 5l7wA

query
sites
5l7wA

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L
x
V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A
|
A

G
|
G

V

H

T
x
H

V

P

P

P

T

P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding [4-fluoranyl-2,3-bis(oxidanyl)phenyl]-[6-(2-fluoranyl-3-oxidanyl-phenyl)pyridin-2-yl]methanone: H90 (≠ T106), S138 (= S151)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), V60 (≠ L77), N86 (= N102), A87 (= A103), G88 (= G104), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 148, 151, 155
binding [4-fluoranyl-2,3-bis(oxidanyl)phenyl]-[6-(2-fluoranyl-3-oxidanyl-phenyl)pyridin-2-yl]methanone: 145, 146, 147, 151, 183, 189, 192
binding beta-D-glucopyranose: 185, 186, 187, 190
binding nicotinamide-adenine-dinucleotide: 13, 16, 17, 18, 151, 155, 181, 182, 183, 184, 186, 188

6qckA 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with fb262 (see paper)
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/258 of 6qckA

query
sites
6qckA

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L
x
V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A

A

G

G

V

H

T
x
H

V

P

P

P

T
x
P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding 2-[2-(1,3-benzodioxol-2-yl)ethyl]benzoic acid: H90 (≠ T106), P93 (≠ T109), S138 (= S151)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), V60 (≠ L77), N86 (= N102), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 151, 155
binding beta-D-glucopyranose: 185, 186, 187, 190
binding 2-[2-(1,3-benzodioxol-2-yl)ethyl]benzoic acid: 145, 151, 188, 189, 192
binding nicotinamide-adenine-dinucleotide: 13, 16, 18, 151, 155, 181, 182, 183, 184, 186, 188

6gtbA 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with fb211
36% identity, 36% coverage: 42:151/306 of query aligns to 31:138/257 of 6gtbA

query
sites
6gtbA

A

A

R

R

V

V

L

V

L

I

A

C

C
x
D

R
x
K

D

D

E

E

S

S

K

G

A

G

R

R

A

A

A

-

M

-

D

-

K

-

I

L

R

E

Q

Q

R

E

S

L

S

P

G

G

A

A

D

-

L

-

A

V

F

F

L

I

P

L

L

C

D
|
D

L
x
V

A

T

D

Q

L

E

A

D

S

D

V

V

R

K

K

T

-

L

A

V

A

S

E

E

V

T

A

I

A

R

K

R

E

F

P

G

R

R

I

L

D

D

V

C

L

V

I

V

N

N

N
|
N

A

A

G

G

V

H

T
x
H

V

P

P

P

T
x
P

R

Q

-

R

-

P

-

E

Q

E

T

T

T

S

R

A

Q

Q

G

G

F

F

E

R

L

Q

L

L

F

L

G

E

V
x
L

N

N

H

L

L

L

G

G

S

T

F

Y

A

T

L

L

T

T

A

K

L

L

M

A

L

L

P

P

K

Y

L

L

R

R

E

K

T

S

P

Q

G

G

S

N

R

V

I

I

V

N

I

I

T

S

S
|
S

active site: S138 (= S151)
binding 3-[6-(3-hydroxyphenyl)pyridin-2-yl]benzoic acid: H90 (≠ T106), P93 (≠ T109), S138 (= S151)
binding nicotinamide-adenine-dinucleotide: D37 (≠ C48), K38 (≠ R49), D59 (= D76), V60 (≠ L77), N86 (= N102), L110 (≠ V123), S138 (= S151)

Sites not aligning to the query:

active site: 17, 151, 155
binding beta-D-glucopyranose: 17, 185, 186, 187, 190
binding 3-[6-(3-hydroxyphenyl)pyridin-2-yl]benzoic acid: 145, 146, 147, 151, 189, 192, 196
binding nicotinamide-adenine-dinucleotide: 13, 16, 17, 18, 151, 155, 181, 182, 183, 184, 186, 188

Query Sequence

>WP_089217430.1 NCBI__GCF_900188185.1:WP_089217430.1
MPSRAGFTAADVPDQSGKCLIVTGANSGVGFEVASALAARRARVLLACRDESKARAAMDK
IRQRSSGADLAFLPLDLADLASVRKAAEVAAKEPRIDVLINNAGVTVPTRQTTRQGFELL
FGVNHLGSFALTALMLPKLRETPGSRIVITSSGQHKGAKIEWDDLNAEKNYNWLSRYGAS
KLANLLFMLELNRRLQAAEMPVTTVGCHPGFARTNFGRASLAGRLAVGVGGLLFNTPAMG
AWPTLQAATGNVKPGGYYGPTAFAELRGPSFECTPSDDARNPQLARRLWDVSIEMTGIDP
DLAPAS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory