SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_089299603.1 NCBI__GCF_900188115.1:WP_089299603.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8vr6A Crystal structure of the pcryo_0619 n-acetryltransferase from psychrobacter cryohalolentis k5 in the presence of coa-disulfide
43% identity, 67% coverage: 26:135/163 of query aligns to 57:165/177 of 8vr6A

query
sites
8vr6A

I

V

T

L

G

G

E

E

P

V

V

K

I

V

G

G

E

K

G

D

T

T

W

W

I

I

G

G

A

P

F

N

T

T

V

I

L

L

D

D

G

G

G

S

G

G

L

G

L

L

R

I

I

I

G

G

D

S

G

N

C

C

D

S

I

I

A

S

A

A

G

G

A

V

Q

Q

I

I

Y
|
Y

T

T

H
|
H

S
x
D

S
x
T

M

V

R

R

R

K

C

S

V

L

S

S

G

G

R

G

A

K

Y

A

D

D

A

-

V

I

D

D

R

K

E

A

A

S

V

T

S

R

I

I

G

G

N

S

H

D

V

C

F

Y

I

L

G

G

P

P

H

N

V

T

T

I

V

I

M
x
V

P

K

G

G

V

V

S

K

V

I

G

G

D

D

S

R

A

V

V

V

L

G

A

A

G

N

S

S

V
x
L

V

V

T
x
L

G

K

D

D

V

I

A

P

A

S

R

D

S

C

L

K

V

V

A

F

G

G

V
x
S

P
|
P

A

A

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: Y97 (= Y66), H99 (= H68), D100 (≠ S69), T101 (≠ S70), V133 (≠ M103), L149 (≠ V119), L151 (≠ T121), S163 (≠ V133), P164 (= P134)

8vr7E Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
43% identity, 67% coverage: 26:135/163 of query aligns to 58:166/180 of 8vr7E

query
sites
8vr7E

I

V

T

L

G

G

E

E

P

V

V

K

I

V

G

G

E

K

G

D

T

T

W
|
W

I

I

G

G

A

P

F

N

T

T

V

I

L

L

D

D

G

G

G

S

G

G

L

G

L

L

R

I

I

I

G

G

D

S

G

N

C

C

D

S

I

I

A
x
S

A

A

G

G

A

V

Q

Q

I

I

Y
|
Y

T

T

H

H

S
x
D

S
x
T

M

V

R

R

R

K

C

S

V

L

S

S

G

G

R

G

A

K

Y

A

D

D

A

-

V

I

D

D

R

K

E

A

A

S

V

T

S

R

I

I

G

G

N

S

H

D

V

C

F
x
Y

I

L

G

G

P

P

H

N

V

T

T

I

V

I

M
x
V

P

K

G

G

V

V

S

K

V

I

G

G

D

D

S

R

A

V

V

V

L
x
G

A
|
A

G

N

S

S

V
x
L

V

V

T
x
L

G

K

D

D

V

I

A

P

A

S

R

D

S

C

L
x
K

V

V

A
x
F

G

G

V
x
S

P
|
P

A

A

binding acetyl coenzyme *a: W69 (= W37), S92 (≠ A60), Y98 (= Y66), D101 (≠ S69), T102 (≠ S70), Y126 (≠ F95), V134 (≠ M103), G146 (≠ L115), A147 (= A116), L150 (≠ V119), L152 (≠ T121), K160 (≠ L129), F162 (≠ A131), S164 (≠ V133), P165 (= P134)

Sites not aligning to the query:

8vr7B Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
43% identity, 67% coverage: 26:135/163 of query aligns to 58:166/177 of 8vr7B

query
sites
8vr7B

I

V

T

L

G

G

E

E

P

V

V

K

I

V

G

G

E

K

G

D

T

T

W

W

I

I

G

G

A

P

F

N

T

T

V

I

L

L

D

D

G

G

G

S

G

G

L

G

L

L

R
x
I

I

I

G

G

D

S

G

N

C

C

D

S

I

I

A

S

A

A

G

G

A

V

Q

Q

I

I

Y
|
Y

T

T

H

H

S
x
D

S
x
T

M

V

R

R

R

K

C

S

V

L

S

S

G

G

R

G

A

K

Y

A

D

D

A

-

V

I

D

D

R

K

E

A

A

S

V

T

S
x
R

I

I

G

G

N

S

H

D

V

C

F
x
Y

I

L

G

G

P
|
P

H

N

V

T

T

I

V

I

M

V

P

K

G

G

V

V

S

K

V

I

G

G

D

D

S

R

A

V

V

V

L
x
G

A
|
A

G

N

S

S

V
x
L

V

V

T
x
L

G

K

D

D

V

I

A

P

A

S

R

D

S

C

L
x
K

V

V

A
x
F

G

G

V

S

P
|
P

A

A

binding acetyl coenzyme *a: Y98 (= Y66), D101 (≠ S69), T102 (≠ S70), L150 (≠ V119), L152 (≠ T121), P165 (= P134)
binding coenzyme a: Y126 (≠ F95), P129 (= P98), G146 (≠ L115), A147 (= A116), K160 (≠ L129), F162 (≠ A131)
binding 3'-phosphate-adenosine-5'-diphosphate: I84 (≠ R52), R120 (≠ S89)

Sites not aligning to the query:

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
40% identity, 56% coverage: 51:142/163 of query aligns to 97:187/188 of 3igjC

query
sites
3igjC

L

V

R

R

I

I

G

G

D

D

G

H

C

C

D

M

I

F

A
|
A

A

P

G

G

A

V

Q

H

I

I

Y
|
Y

T

T

-
x
A

-

T

-
x
H

-

P

-

L

H

H

S

P

S

V

M

E

R

R

R

N

C

-

V

-

S

S

G

G

R

K

A

E

Y

Y

D

G

A

-

V

-

D

-

R

-

E

K

A

P

V

V

S

K

I

I

G

G

N

N

H

N

V

V

F

W

I

V

G
|
G

P

G

H

G

V

A

T

I

V

I

M
x
N

P

P

G

G

V

V

S

S

V

I

G

G

D

D

S

N

A

A

A

V

V

I

L
x
A

A
x
S

G

G

S

A

V

V

T
|
T

G

K

D

D

V

V

A

P

A

N

R

N

S

V

L

V

V

V

A

G

G

G

V
x
N

P

P

A

A

R

K

P

V

I

I

G

K

H

T

L

I

D

E

binding acetyl coenzyme *a: A106 (= A60), Y112 (= Y66), A114 (vs. gap), H116 (vs. gap), G142 (= G97), N148 (≠ M103), A160 (≠ L115), S161 (≠ A116), T166 (= T121), N178 (≠ V133)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
36% identity, 55% coverage: 53:142/163 of query aligns to 97:185/200 of 1krrA

query
sites
1krrA

I

I

G

G

D

D

G

N

C

V

D

L

I
|
I

A
|
A

A
x
P

G

N

A

V

Q

T

I

L

Y

S

T

V

-
x
T

-

G

-
x
H

-

P

-

V

H

H

S

H

S

E

M

L

R

R

R

K

C

-

V

-

S

N

G

G

R

E

A

M

Y

Y

D

S

A

F

V

-

D

-

R

-

E

-

A

P

V

I

S

T

I

I

G

G

N

N

H

N

V

V

F

W

I

I

G
|
G

P
x
S

H

H

V

V

T

V

V

I

M
x
N

P

P

G

G

V

V

S

T

V

I

G

G

D

D

S

N

A

S

A

V

V

I

L
x
G

A
|
A

G

G

S

S

V

I

V

V

T

T

G

K

D

D

V

I

A

P

R

N

S

V

L

V

V

A

A
|
A

G

G

V

V

P
|
P

A

C

R

R

P

V

I

I

G
x
R

H

E

L

I

D

N

binding acetyl coenzyme *a: I103 (= I59), A104 (= A60), P105 (≠ A61), T112 (vs. gap), H114 (vs. gap), G140 (= G97), S141 (≠ P98), N146 (≠ M103), G158 (≠ L115), A159 (= A116), A174 (= A131), P177 (= P134), R182 (≠ G139)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
36% identity, 55% coverage: 53:142/163 of query aligns to 97:185/201 of 1krvA

query
sites
1krvA

I

I

G

G

D

D

G

N

C

V

D

L

I

I

A
|
A

A

P

G

N

A

V

Q

T

I

L

Y
x
S

T

V

-
x
T

-

G

-

H

-

P

-

V

H

H

S

H

S

E

M

L

R

R

R

K

C

-

V

-

S

N

G

G

R

E

A
x
M

Y

Y

D

S

A

F

V

-

D

-

R

-

E

-

A

P

V

I

S

T

I

I

G

G

N

N

H

N

V

V

F
x
W

I

I

G
|
G

P
x
S

H

H

V

V

T

V

V

I

M
x
N

P

P

G

G

V

V

S

T

V

I

G

G

D

D

S

N

A

S

A

V

V

I

L

G

A
|
A

G

G

S

S

V

I

V

V

T

T

G

K

D

D

V

I

A

P

R

N

S

V

L

V

V

A

A

A

G

G

V

V

P
|
P

A

C

R
|
R

P

V

I

I

G
x
R

H

E

L

I

D

N

binding 4-nitrophenyl beta-D-galactopyranoside: M126 (≠ A79)
binding coenzyme a: A104 (= A60), S110 (≠ Y66), T112 (vs. gap), W138 (≠ F95), G140 (= G97), S141 (≠ P98), N146 (≠ M103), A159 (= A116), P177 (= P134), R179 (= R136), R182 (≠ G139)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70, 82, 84, 90, 92

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
36% identity, 55% coverage: 53:142/163 of query aligns to 97:185/201 of 1kruA

query
sites
1kruA

I

I

G

G

D

D

G

N

C

V

D
x
L

I

I

A

A

A

P

G

N

A

V

Q

T

I

L

Y

S

T

V

-

T

-

G

-
x
H

-

P

-

V

H

H

S

H

S

E

M

L

R

R

R

K

C

-

V

-

S

N

G

G

R

E

A
x
M

Y

Y

D

S

A

F

V

-

D

-

R

-

E

-

A

P

V

I

S

T

I

I

G

G

N

N

H

N

V

V

F
x
W

I

I

G
|
G

P
x
S

H

H

V

V

T

V

V

I

M

N

P

P

G

G

V

V

S

T

V

I

G

G

D

D

S

N

A

S

A

V

V

I

L
x
G

A
|
A

G

G

S

S

V

I

V

V

T

T

G

K

D

D

V

I

A

P

R

N

S

V

L

V

V

A

A

A

G

G

V

V

P
|
P

A

C

R

R

P

V

I

I

G
x
R

H

E

L

I

D

N

binding coenzyme a: H114 (vs. gap), W138 (≠ F95), G140 (= G97), S141 (≠ P98), G158 (≠ L115), A159 (= A116), P177 (= P134), R182 (≠ G139)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: L102 (≠ D58), H114 (vs. gap), M126 (≠ A79)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70, 82, 82, 84, 90, 92

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 55% coverage: 53:142/163 of query aligns to 98:186/203 of P07464

query
sites
P07464

I

I

G

G

D

D

G

N

C

V

D

L

I

I

A

A

A

P

G

N

A

V

Q

T

I

L

Y

S

T

V

-

T

-

G

-
x
H

-

P

-

V

H

H

S

H

S

E

M

L

R

R

R

K

C

-

V

-

S

N

G

G

R

E

A

M

Y

Y

D

S

A

F

V

-

D

-

R

-

E

-

A

P

V

I

S

T

I

I

G

G

N

N

H

N

V

V

F

W

I

I

G

G

P
x
S

H

H

V

V

T

V

V

I

M

N

P

P

G

G

V

V

S

T

V

I

G

G

D

D

S

N

A

S

A

V

V

I

L

G

A
|
A

G

G

S

S

V

I

V

V

T
|
T

G
x
K

D

D

V

I

A

P

R

N

S

V

L

V

V

A

A

A

G

G

V

V

P

P

A

C

R
|
R

P

V

I

I

G
x
R

H

E

L

I

D

N

H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ P98) binding in other chain
A160 (= A116) binding in other chain
TK 165:166 (≠ TG 121:122) binding
R180 (= R136) binding
R183 (≠ G139) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding
85 binding in other chain; binding in other chain
93 binding

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
35% identity, 56% coverage: 51:142/163 of query aligns to 95:185/186 of 4isxA

query
sites
4isxA

L

I

R

E

I

I

G

G

D

D

G

N

C

V

D

M

I

L

A
|
A

A

P

G

N

A

V

Q

Q

I

I

Y

Y

T

T

-

A

-

Y

-

H

-

P

-

I

H

D

S

A

S

Q

M

L

R

R

R

N

C

-

V

-

S

S

G

G

R

I

A

E

Y

Y

D

G

A

S

V

-

D

-

R

-

E

-

A

P

V

V

S

K

I

I

G

G

N

D

H

N

V

V

F
x
W

I

I

G
|
G

P
x
G

H

G

V

V

T

I

V

I

M

T

P

P

G

G

V

I

S

T

V

I

G

G

D

D

S

N

A

V

V

I

L

G

A
|
A

G

G

S

S

V

V

T

T

G

K

D

D

V

I

A

P

R

N

S

T

L

V

V

A

A
x
V

G

G

V

N

P

P

A

C

R

R

P

V

I

I

G
x
K

H

K

L

I

D

E

binding acetyl coenzyme *a: A104 (= A60), W138 (≠ F95), G140 (= G97), G141 (≠ P98), A159 (= A116), V174 (≠ A131), K182 (≠ G139)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
34% identity, 55% coverage: 51:140/163 of query aligns to 61:166/209 of P50870

query
sites
P50870

L

L

R

K

I

I

G

G

D

K

G

F

C

C

D

S

I

I

A

G

A

P

G

G

A

V

Q

T

I

I

Y

I

T

M

H

N

S

G

S

A

M

N

R
x
H

R

R

C

M

-

D

-

G

-

S

-

T

-

Y

-

P

-

F

-

N

V

L

S

F

G

G

R

N

A

G

Y

W

D

E

A

K

-

H

-

M

-

P

-

K

V

L

D

D

R

Q

E

L

A

P

V

I

S

K

-

G

-

D

-

T

-

I

I

I

G

G

N

N

H

D

V

V

F

W

I

I

G

G

P

K

H

D

V

V

T

V

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

V

L

A

A

A

G

N

S

S

V

V

T

V

G

K

D

D

V

I

A

A

A

P

R

Y

S

M

L

L

V

A

A

G

G

G

V

N

P

P

A

A

R

N

P

E

I

I

G

K

H

Q

H82 (≠ R72) mutation to A: 105-fold decrease in activity.

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
34% identity, 55% coverage: 51:140/163 of query aligns to 61:166/206 of 1khrA

query
sites
1khrA

L

L

R

K

I

I

G

G

D

K

G

F

C

C

D

S

I

I

A

G

A

P

G

G

A

V

Q

T

I

I

Y

I

T

M

H

N

S

G

S
x
A

M
x
N

R
x
H

R

R

C
x
M

-

D

-

G

-

S

-

T

-

Y

-

P

-

F

-

N

V
x
L

S

F

G

G

R

N

A

G

Y

W

D

E

A

K

-

H

-

M

-

P

-

K

V

L

D

D

R

Q

E

L

A

P

V

I

S

K

-

G

-

D

-

T

-

I

I

I

G

G

N

N

H

D

V

V

F
x
W

I

I

G

G

P

K

H

D

V

V

T

V

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

V

L
x
A

A
|
A

G

N

S

S

V

V

T
x
V

G

K

D

D

V

I

A

A

A

P

R

Y

S

M

L
|
L

V

A

A
x
G

G

G

V

N

P
|
P

A

A

R

N

P

E

I
|
I

G

K

H

Q

binding coenzyme a: H82 (≠ R72), M84 (≠ C74), W121 (≠ F95), A141 (≠ L115), A142 (= A116), V147 (≠ T121), L155 (= L129), G157 (≠ A131), P160 (= P134), I164 (= I138)
binding virginiamycin m1: A80 (≠ S70), N81 (≠ M71), H82 (≠ R72), L93 (≠ V75)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
34% identity, 55% coverage: 51:140/163 of query aligns to 61:166/203 of 3dhoA

query
sites
3dhoA

L

L

R

K

I

I

G

G

D

K

G

F

C

C

D

S

I

I

A

G

A

P

G

G

A

V

Q

T

I

I

Y

I

T

M

H

N

S

G

S

A

M

N

R

H

R

R

C
x
M

-

D

-

G

-

S

-

T

-

Y

-

P

-

F

-

N

V

L

S

F

G

G

R

N

A

G

Y

W

D

E

A

K

-

H

-

M

-

P

-

K

V

L

D

D

R

Q

E

L

A

P

V

I

S

K

-

G

-

D

-

T

-

I

I

I

G

G

N

N

H

D

V

V

F

W

I

I

G

G

P

K

H

D

V

V

T

V

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A
x
I

V

V

L
x
A

A

A

G

N

S

S

V

V

T
x
V

G

K

D

D

V

I

A

A

A

P

R

Y

S

M

L
|
L

V

A

A
x
G

G

G

V

N

P
|
P

A

A

R

N

P

E

I
|
I

G

K

H

Q

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ C74), I139 (≠ A113), A141 (≠ L115), V147 (≠ T121), L155 (= L129), G157 (≠ A131), P160 (= P134), I164 (= I138)

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
34% identity, 55% coverage: 51:140/163 of query aligns to 61:166/205 of 1kk4A

query
sites
1kk4A

L

L

R

K

I

I

G

G

D

K

G

F

C

C

D

S

I
|
I

A
x
G

A

P

G

G

A

V

Q

T

I

I

Y

I

T

M

H

N

S

G

S

A

M

N

R

H

R

R

C

M

-

D

-

G

-

S

-

T

-

Y

-

P

-

F

-

N

V

L

S

F

G

G

R

N

A

G

Y

W

D

E

A

K

-

H

-

M

-

P

-

K

V

L

D

D

R

Q

E

L

A

P

V

I

S
x
K

-

G

-

D

-

T

-

I

I

I

G

G

N

N

H

D

V

V

F
x
W

I

I

G
|
G

P
x
K

H

D

V

V

T

V

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

V

L
x
A

A
|
A

G

N

S

S

V

V

T
x
V

G
x
K

D

D

V

I

A

A

A

P

R

Y

S

M

L
|
L

V

A

A
x
G

G
|
G

V

N

P
|
P

A

A

R

N

P

E

I
|
I

G

K

H

Q

binding acetyl coenzyme *a: I69 (= I59), G70 (≠ A60), K111 (≠ S89), W121 (≠ F95), G123 (= G97), K124 (≠ P98), A141 (≠ L115), A142 (= A116), V147 (≠ T121), K148 (≠ G122), L155 (= L129), G157 (≠ A131), G158 (= G132), P160 (= P134), I164 (= I138)

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
34% identity, 55% coverage: 51:140/163 of query aligns to 61:166/204 of 1mrlA

query
sites
1mrlA

L

L

R

K

I

I

G

G

D

K

G

F

C

C

D

S

I

I

A

G

A

P

G

G

A

V

Q

T

I

I

Y

I

T

M

H

N

S

G

S

A

M
x
N

R
x
H

R

R

C

M

-

D

-

G

-

S

-

T

-

Y

-

P

-

F

-

N

V
x
L

S

F

G

G

R

N

A

G

Y

W

D

E

A

K

-

H

-
x
M

-

P

-

K

V

L

D

D

R

Q

E
x
L

A

P

V

I

S

K

-

G

-

D

-

T

-

I

I

I

G

G

N

N

H

D

V

V

F

W

I

I

G

G

P

K

H

D

V

V

T

V

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

V

L

A

A

A

G

N

S

S

V

V

T

V

G

K

D

D

V

I

A

A

A

P

R

Y

S

M

L

L

V

A

A

G

G

G

V

N

P

P

A

A

R

N

P

E

I

I

G

K

H

Q

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (≠ M71), H82 (≠ R72), L93 (≠ V75), M102 (vs. gap), L108 (≠ E86)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
33% identity, 61% coverage: 48:146/163 of query aligns to 92:189/190 of 5u2kA

query
sites
5u2kA

G

G

L

Q

L

I

R

T

I

I

G

G

D

D

G

N

C

V

D

F

I

I

A

G

A

P

G

N

A

C

Q

G

I

F

Y
|
Y

T

T

H

-

S
x
A

S

T

M
x
H

R

P

R

L

C

N

V

F

S

H

G

H

R

R

A

N

Y

E

D

G

A

F

V

E

D

K

R

A

E

G

A

P

V

I

S

H

I

I

G

G

N

S

H

N

V

T

F

W

I

F

G

G

P

G

H

H

V

V

T

A

V

V

M
x
L

P

P

G

G

V

V

S

T

V

I

G

G

D

E

S

G

A

S

A

V

V

I

L

G

A

A

G

G

S

S

V

V

T
|
T

G
x
K

D

D

V

I

A

P

R

H

S

S

L

L

V

A

A

V

G

G

V

N

P

P

A

C

R

K

P

V

I

V

G

R

H

K

L

I

D

D

I

N

D

D

G

L

P

P

binding coenzyme a: Y110 (= Y66), A112 (≠ S69), H114 (≠ M71), L146 (≠ M103), T164 (= T121), K165 (≠ G122)

Sites not aligning to the query:

binding coenzyme a: 90

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
51% identity, 31% coverage: 88:138/163 of query aligns to 113:163/212 of 4husA

query
sites
4husA

V

I

S

E

I

I

G

G

N

N

H

D

V

V

F

W

I

I

G

G

P

R

H

D

V

V

T

T

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

I

L

A

A

A

G

E

S

A

V

V

T

T

G

K

D

N

V

V

A

A

A

P

R

Y

S

S

L

I

V

V

A

G

G

G

V

N

P

P

A

L

R

K

P

F

I

I

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
51% identity, 31% coverage: 88:138/163 of query aligns to 113:163/211 of 4hurA

query
sites
4hurA

V

I

S

E

I

I

G

G

N

N

H

D

V

V

F
x
W

I
|
I

G
|
G

P
x
R

H

D

V

V

T

T

V

I

M
|
M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

I

L
x
A

A
|
A

G

E

S

A

V

V

T
|
T

G

K

D

N

V

V

A

A

A

P

R

Y

S

S

L

I

V

V

A
x
G

G

G

V

N

P
|
P

A

L

R

K

P

F

I
|
I

binding acetyl coenzyme *a: W120 (≠ F95), I121 (= I96), G122 (= G97), R123 (≠ P98), M128 (= M103), A140 (≠ L115), A141 (= A116), T146 (= T121), G156 (≠ A131), P159 (= P134), I163 (= I138)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51, 67, 68, 69, 79, 80, 110, 164

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
48% identity, 36% coverage: 88:145/163 of query aligns to 113:170/207 of 6x3cA

query
sites
6x3cA

V

I

S

E

I

I

G

G

N

N

H

D

V

V

F
x
W

I

I

G
|
G

P
x
R

H

D

V

V

T

T

V

I

M
|
M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

I

L
x
A

A
|
A

G

E

S

A

V

V

T

T

G

K

D

N

V

V

A

A

A

P

R

Y

S

S

L

I

V

V

A

G

G

G

V

N

P

P

A

L

R

K

P

F

I

I

G

R

H

K

L

R

D

F

I

S

D

D

G

G

binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (≠ F95), G122 (= G97), R123 (≠ P98), M128 (= M103), A140 (≠ L115), A141 (= A116)

Sites not aligning to the query:

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
51% identity, 31% coverage: 88:138/163 of query aligns to 113:163/206 of 6x3jA

query
sites
6x3jA

V

I

S

E

I

I

G

G

N

N

H

D

V

V

F
x
W

I

I

G
|
G

P

R

H

D

V

V

T

T

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

I

L

A

A

A

G

E

S

A

V

V

T

T

G

K

D

N

V

V

A

A

A

P

R

Y

S

S

L

I

V

V

A

G

G

G

V

N

P

P

A

L

R

K

P

F

I

I

binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (≠ F95), G122 (= G97)

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
51% identity, 31% coverage: 88:138/163 of query aligns to 113:163/203 of 6x3cE

query
sites
6x3cE

V

I

S

E

I

I

G

G

N

N

H

D

V

V

F
x
W

I

I

G
|
G

P

R

H

D

V

V

T

T

V

I

M

M

P

P

G

G

V

V

S

K

V

I

G

G

D

D

S

G

A

A

A

I

V

I

L
x
A

A
|
A

G

E

S

A

V

V

T

T

G

K

D

N

V

V

A

A

A

P

R

Y

S

S

L

I

V

V

A

G

G

G

V
x
N

P
|
P

A

L

R

K

P

F

I

I

binding magnesium ion: N158 (≠ V133), P159 (= P134)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (≠ F95), G122 (= G97), A140 (≠ L115), A141 (= A116)

Sites not aligning to the query:

Query Sequence

>WP_089299603.1 NCBI__GCF_900188115.1:WP_089299603.1
MRGGFSAAFHAEHDIPPNPYNAHARITGEPVIGEGTWIGAFTVLDGGGGLLRIGDGCDIA
AGAQIYTHSSMRRCVSGRAYDAVDREAVSIGNHVFIGPHVTVMPGVSVGDSAAVLAGSVV
TGDVAARSLVAGVPARPIGHLDIDGPEVRVVYDRAGQDPGRAV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory