SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_089300362.1 NCBI__GCF_900188115.1:WP_089300362.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
28% identity, 65% coverage: 66:269/312 of query aligns to 184:368/409 of O53289

query
sites
O53289

F

F

D
|
D

V
|
V

D
|
D

N
x
S

T

T

M

L

M

V

M

Q

G

G

A

E

S

V

I

I

F

-

H

-

F

-

A

-

R

E

G

M

L

L

A

A

R

R

K

-

Y

-

F

-

T

-

S

-

D

-

L

-

A

A

G

G

F

A

A

Q

W

G

R

Q

Q

V

A

A

R

A

F

I

R

T

I

E

G

A

K

M

E

R

T

G

K

E

D

L

T

D

V

F

S

A

S

E

S

S

R

L

E

Q

Q

R

A

R

L

V

S

A

F

T

V

L

A

A

G

G

R

L

S

P

V

A

E

T

E

V

L

I

N

D

T

D

V

V

G

A

E

E

E

Q

I

L

Y

-

D

-

E

E

L

L

M

M

A

-

D

-

K

-

I

-

W

-

S

P

G

G

T

A

R

R

A

T

L

T

A

I

Q

R

M

T

H

L

L

R

D

R

A

L

G

G

Q

F

R

R

V

C

W

G

L

V

V

V

T
x
S

A

G

T

G

P

F

V

R

E

R

L

I

A

I

A

E

I

P

I

L

A

A

R

R

R

E

L

L

G

M

L

L

T

D

G

F

A

V

L

A

G

S

T

N

V

E

A

L

E

E

T

I

D

V

D

D

G

G

V

I

Y

L

T

T

G

G

K

R

L

V

V

V

G

G

D

P

L

I

L

V

H

D

G

R

R

P

A

G

K
|
K

A

A

H

K

A

A

V

L

R

R

A

D

L

F

A

A

A

S

K

Q

E

Y

G

G

L

V

N

P

L

M

K

E

R

Q

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N

N

D
|
D

V

I

P

D

M

M

L

L

S

G

V

A

T

A

G

G

T

L

A

G

V

I

A

A

I

F

N

N

P

A

D

K

A

P

G

A

L

L

R

R

E

E

V

V

A

A

D185 (= D67) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (= V68) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D69) mutation to N: Decreases enzymatic activity by 15%.
S188 (≠ N70) mutation to A: No effect on enzymatic activity.
S273 (≠ T174) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K219) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D242) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D246) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
38% identity, 23% coverage: 199:269/312 of query aligns to 296:366/396 of 8a21A

query
sites
8a21A

E

E

T

I

D

V

D

D

G

G

V

T

Y

L

T

T

G

G

K

R

L

V

V

V

G

G

D

P

L

I

H

D

G

R

R

A

A

G

K

K

A

A

H

T

A

A

V

L

R

R

A

E

L

F

A

A

A

Q

K

R

E

A

G

G

L

V

N

P

L

M

K

A

R

Q

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N

N

D

D

V

I

P

D

M

M

L

L

S

A

V

A

T

A

G

G

T

L

A

G

V

I

A

A

I

F

N

N

P

A

D

K

A

P

G

A

L

L

R

R

E

E

V

V

A

A

binding magnesium ion: D339 (= D242)

Sites not aligning to the query:

binding magnesium ion: 183, 185
binding 4-phenyl-1h-imidazole: 13, 18, 37, 185, 192, 193, 194, 211, 215, 221, 228, 273

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
38% identity, 23% coverage: 199:269/312 of query aligns to 296:366/396 of 8a1zA

query
sites
8a1zA

E

E

T

I

D

V

D

D

G

G

V

T

Y

L

T

T

G

G

K

R

L

V

V

V

G

G

D

P

L

I

H

D

G

R

R

A

A

G

K

K

A

A

H

T

A

A

V

L

R

R

A

E

L

F

A

A

A

Q

K

R

E

A

G

G

L

V

N

P

L

M

K

A

R

Q

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N

N

D

D

V

I

P

D

M

M

L

L

S

A

V

A

T

A

G

G

T

L

A

G

V

I

A

A

I

F

N

N

P

A

D

K

A

P

G

A

L

L

R

R

E

E

V

V

A

A

Sites not aligning to the query:

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: 185, 192, 215, 221, 225, 228, 272, 274
binding magnesium ion: 183, 185

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
38% identity, 23% coverage: 199:269/312 of query aligns to 296:366/396 of 5jlpA

query
sites
5jlpA

E

E

T

I

D

V

D

D

G

G

V

T

Y

L

T

T

G

G

K

R

L

V

V

V

G

G

D

P

L

I

H

D

G

R

R

A

A

G

K
|
K

A

A

H

T

A

A

V

L

R

R

A

E

L

F

A

A

A

Q

K

R

E

A

G

G

L

V

N

P

L

M

K

A

R

Q

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N

N

D
|
D

V

I

P

D

M

M

L

L

S

A

V

A

T

A

G

G

T

L

A

G

V

I

A

A

I

F

N

N

P

A

D

K

A

P

G

A

L

L

R

R

E

E

V

V

A

A

active site: K316 (= K219), D343 (= D246)
binding magnesium ion: D339 (= D242)

Sites not aligning to the query:

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
38% identity, 23% coverage: 199:269/312 of query aligns to 300:370/411 of A0QJI1

query
sites
A0QJI1

E

E

T

I

D

V

D

D

G

G

V

T

Y

L

T

T

G

G

K

R

L

V

V

V

G

G

D

P

L

I

H

D

G

R

R

A

A

G

K

K

A

A

H

T

A

A

V

L

R

R

A

E

L

F

A

A

A

Q

K

R

E

A

G

G

L

V

N

P

L

M

K

A

R

Q

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N

N

D

D

V

I

P

D

M

M

L

L

S

A

V

A

T

A

G

G

T

L

A

G

V

I

A

A

I

F

N

N

P

A

D

K

A

P

G

A

L

L

R

R

E

E

V

V

A

A

D343 (= D242) binding Mg(2+)

Sites not aligning to the query:

187 binding Mg(2+)
189 binding Mg(2+)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
33% identity, 31% coverage: 184:280/312 of query aligns to 109:204/211 of Q58989

query
sites
Q58989

I

I

A

K

R

E

R

K

L

L

G

G

L

L

T

D

G

Y

A

A

L

F

G

A

T

N

V

R

A

L

E

I

T

V

D

K

D

D

G

G

V

K

Y

L

T

T

G

G

K

D

L

V

V

E

G

G

D

E

L

V

L

L

H

K

G

E

R

N

A

A

K
|
K

A

G

H

E

A

I

V

L

R

E

A

K

L

I

A

A

A

K

K

I

E

E

G

G

L

I

N

N

L

L

K

E

R

D

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N
|
N

D

D

V

I

P

S

M

M

L

F

S

K

V

K

T

A

G

G

T

L

A

K

V

I

A

A

I

F

N

C

P

A

D

K

A

P

G

I

L

L

R

K

E

E

V

K

A

A

R

-

Q

D

R

I

Q

C

W

I

E

E

I

K

R

R

D

D

F

L

R

R

K144 (= K219) binding substrate
D167 (= D242) binding Mg(2+)
N170 (= N245) binding substrate

Sites not aligning to the query:

11 active site, Nucleophile; binding Mg(2+); D→N: Loss of activity.
13 active site, Proton donor; binding Mg(2+)
20 binding substrate
56 binding substrate
99:100 binding substrate

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
33% identity, 31% coverage: 184:280/312 of query aligns to 107:202/209 of 1l7nA

query
sites
1l7nA

I

I

A

K

R

E

R

K

L

L

G

G

L

L

T

D

G

Y

A

A

L

F

G

A

T

N

V

R

A

L

E

I

T

V

D

K

D

D

G

G

V

K

Y

L

T

T

G

G

K

D

L

V

V

E

G

G

D

E

L

V

L

L

H

K

G

E

R

N

A

A

K
|
K

A

G

H

E

A

I

V

L

R

E

A

K

L

I

A

A

A

K

K

I

E

E

G

G

L

I

N

N

L

L

K

E

R

D

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N
|
N

D
|
D

V

I

P

S

M

M

L

F

S

K

V

K

T

A

G

G

T

L

A

K

V

I

A

A

I

F

N

C

P

A

D

K

A

P

G

I

L

L

R

K

E

E

V

K

A

A

R

-

Q

D

R

I

Q

C

W

I

E

E

I

K

R

R

D

D

F

L

R

R

active site: K142 (= K219), D169 (= D246)
binding aluminum fluoride: K142 (= K219)
binding tetrafluoroaluminate ion: K142 (= K219), N168 (= N245)
binding magnesium ion: D165 (= D242)

Sites not aligning to the query:

active site: 9, 10, 11, 98
binding aluminum fluoride: 9, 10, 11, 97
binding tetrafluoroaluminate ion: 9, 10, 11, 97, 98
binding magnesium ion: 9, 11

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
33% identity, 31% coverage: 184:280/312 of query aligns to 108:203/210 of 1f5sA

query
sites
1f5sA

I

I

A

K

R

E

R

K

L

L

G

G

L

L

T

D

G

Y

A

A

L

F

G

A

T

N

V

R

A

L

E

I

T

V

D

K

D

D

G

G

V

K

Y

L

T

T

G

G

K

D

L

V

V

E

G

G

D

E

L

V

L

L

H

K

G

E

R

N

A

A

K
|
K

A

G

H

E

A

I

V

L

R

E

A

K

L

I

A

A

A

K

K

I

E

E

G

G

L

I

N

N

L

L

K

E

R

D

C

T

T

V

A

A

Y

V

S

G

D
|
D

S

G

Q

A

N

N

D
|
D

V

I

P

S

M

M

L

F

S

K

V

K

T

A

G

G

T

L

A

K

V

I

A

A

I

F

N

C

P

A

D

K

A

P

G

I

L

L

R

K

E

E

V

K

A

A

R

-

Q

D

R

I

Q

C

W

I

E

E

I

K

R

R

D

D

F

L

R

R

active site: K143 (= K219), D170 (= D246)
binding magnesium ion: D166 (= D242)
binding phosphate ion: K143 (= K219)

Sites not aligning to the query:

active site: 10, 11, 12, 99
binding magnesium ion: 10, 12
binding phosphate ion: 10, 11, 12, 98, 99

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
33% identity, 31% coverage: 184:280/312 of query aligns to 106:201/208 of 1l7pA

query
sites
1l7pA

I

I

A

K

R

E

R

K

L

L

G

G

L

L

T

D

G

Y

A

A

L

F

G

A

T

N

V

R

A

L

E

I

T

V

D

K

D

D

G

G

V

K

Y

L

T

T

G

G

K

D

L

V

V

E

G

G

D

E

L

V

L

L

H

K

G

E

R

N

A

A

K
|
K

A

G

H

E

A

I

V

L

R

E

A

K

L

I

A

A

A

K

K

I

E

E

G

G

L

I

N

N

L

L

K

E

R

D

C

T

T

V

A

A

Y

V

S

G

D

D

S

G

Q

A

N

N

D
|
D

V

I

P

S

M

M

L

F

S

K

V

K

T

A

G

G

T

L

A

K

V

I

A

A

I

F

N

C

P

A

D

K

A

P

G

I

L

L

R

K

E

E

V

K

A

A

R

-

Q

D

R

I

Q

C

W

I

E

E

I

K

R

R

D

D

F

L

R

R

active site: K141 (= K219), D168 (= D246)
binding phosphoserine: K141 (= K219)

Sites not aligning to the query:

active site: 8, 9, 10, 97
binding phosphoserine: 8, 9, 10, 17, 40, 46, 53, 96, 97

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
33% identity, 33% coverage: 184:287/312 of query aligns to 98:200/200 of 1l7oA

query
sites
1l7oA

I

I

A

K

R

E

R

K

L

L

G

G

L

L

T

D

G

Y

A

A

L

F

G

A

T

N

V

R

A

L

E

I

T

V

D

K

D

D

G

G

V

K

Y

L

T

T

G

G

K

D

L

V

V

E

G

G

D

E

L

V

L

L

H

K

G

E

R

N

A

A

K

K

A

G

H

E

A

I

V

L

R

E

A

K

L

I

A

A

A

K

K

I

E

E

G

G

L

I

N

N

L

L

K

E

R

D

C

T

T

V

A

A

Y

V

S

G

D

D

S

G

Q

A

N

N

D

D

V

I

P

S

M

M

L

F

S

K

V

K

T

A

G

G

T

L

A

K

V

I

A

A

I

F

N

C

P

A

D

K

A

P

G

I

L

L

R

K

E

E

V

K

A

A

R

-

Q

D

R

I

Q
x
C

W

I

E
|
E

I

K

R

R

D

D

F

L

R

R

T

E

G

I

R

L

K

K

A

Y

A

I

K

K

binding zinc ion: C186 (≠ Q273), E188 (= E275)

Query Sequence

>WP_089300362.1 NCBI__GCF_900188115.1:WP_089300362.1
MSLWRRKAKRHELERLATLAGEASAEAASRMDLAAQREGDEPEGHAERSATAGPPAPPDL
TAAAFFDVDNTMMMGASIFHFARGLAARKYFTTSDLAGFAWRQARFRIGGKETKDTVSSS
REQALSFVAGRSVEELNTVGEEIYDELMADKIWSGTRALAQMHLDAGQRVWLVTATPVEL
AAIIARRLGLTGALGTVAETDDGVYTGKLVGDLLHGRAKAHAVRALAAKEGLNLKRCTAY
SDSQNDVPMLSVTGTAVAINPDAGLREVARQRQWEIRDFRTGRKAAKIGVPSVLGAGALA
GAVAAGVAFRKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory