SitesBLAST
Comparing WP_089303491.1 NCBI__GCF_900188115.1:WP_089303491.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
42% identity, 86% coverage: 35:270/275 of query aligns to 3:239/240 of 1ji0A
6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
32% identity, 84% coverage: 38:269/275 of query aligns to 2:235/240 of 6mjpA
1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
31% identity, 83% coverage: 43:269/275 of query aligns to 8:253/254 of 1g6hA
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
31% identity, 83% coverage: 43:269/275 of query aligns to 8:253/253 of 1g9xB
6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
33% identity, 79% coverage: 54:269/275 of query aligns to 17:235/238 of 6s8nB
6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
33% identity, 79% coverage: 54:269/275 of query aligns to 17:235/238 of 6s8gA
Sites not aligning to the query:
6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
33% identity, 79% coverage: 54:269/275 of query aligns to 17:235/235 of 6mhzA
- binding adp orthovanadate: N37 (= N74), G38 (= G75), G40 (= G77), K41 (= K78), T42 (≠ S79), T43 (= T80), Q84 (= Q121), S136 (= S170), S138 (= S172), G139 (= G173), G140 (= G174), E162 (= E196), G166 (= G200), H194 (≠ Q228)
Sites not aligning to the query:
6mbnA Lptb e163q in complex with atp (see paper)
33% identity, 79% coverage: 54:269/275 of query aligns to 18:236/241 of 6mbnA
Sites not aligning to the query:
6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
33% identity, 78% coverage: 54:268/275 of query aligns to 17:234/234 of 6b89A
- binding adenosine-5'-diphosphate: V17 (= V54), N37 (= N74), G38 (= G75), G40 (= G77), K41 (= K78), T42 (≠ S79), T43 (= T80)
- binding magnesium ion: T42 (≠ S79), Q84 (= Q121)
- binding novobiocin: L71 (≠ T108), H72 (= H109), P83 (= P120), A86 (≠ D123), S87 (≠ N124), F89 (= F126), R90 (≠ P127), R91 (≠ A128), L92 (= L129), V101 (vs. gap), Q135 (≠ G169), R149 (= R183)
Sites not aligning to the query:
4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
33% identity, 78% coverage: 54:268/275 of query aligns to 17:234/234 of 4p31A
Sites not aligning to the query:
6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
33% identity, 78% coverage: 54:267/275 of query aligns to 17:233/233 of 6b8bA
- binding adenosine-5'-diphosphate: V17 (= V54), G38 (= G75), G40 (= G77), K41 (= K78), T42 (≠ S79), T43 (= T80)
- binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (= F126), R90 (≠ P127), R91 (≠ A128)
Sites not aligning to the query:
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
34% identity, 77% coverage: 51:261/275 of query aligns to 14:224/374 of 2awnB
Sites not aligning to the query:
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
34% identity, 77% coverage: 51:261/275 of query aligns to 15:225/371 of P68187
- A85 (≠ N124) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (= K142) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ F150) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (= V153) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E155) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A161) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G174) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D195) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 77% coverage: 51:261/275 of query aligns to 15:225/369 of P19566
- L86 (≠ V125) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P197) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (≠ S202) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
34% identity, 77% coverage: 51:261/275 of query aligns to 14:224/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: S37 (≠ N74), G38 (= G75), C39 (≠ A76), G40 (= G77), K41 (= K78), S42 (= S79), T43 (= T80), Q81 (= Q121), R128 (≠ Q166), A132 (≠ S170), S134 (= S172), G136 (= G174), Q137 (≠ E175), E158 (= E196), H191 (≠ Q228)
- binding magnesium ion: S42 (= S79), Q81 (= Q121)
Sites not aligning to the query:
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
34% identity, 77% coverage: 51:261/275 of query aligns to 14:224/371 of 3puxA
- binding adenosine-5'-diphosphate: G38 (= G75), C39 (≠ A76), G40 (= G77), K41 (= K78), S42 (= S79), T43 (= T80), R128 (≠ Q166), S134 (= S172), Q137 (≠ E175)
- binding beryllium trifluoride ion: S37 (≠ N74), G38 (= G75), K41 (= K78), Q81 (= Q121), S134 (= S172), G136 (= G174), H191 (≠ Q228)
- binding magnesium ion: S42 (= S79), Q81 (= Q121)
Sites not aligning to the query:
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
34% identity, 77% coverage: 51:261/275 of query aligns to 14:224/371 of 3puwA
- binding adenosine-5'-diphosphate: V17 (= V54), G38 (= G75), C39 (≠ A76), G40 (= G77), K41 (= K78), S42 (= S79), T43 (= T80), R128 (≠ Q166), A132 (≠ S170), S134 (= S172), Q137 (≠ E175)
- binding tetrafluoroaluminate ion: S37 (≠ N74), G38 (= G75), K41 (= K78), Q81 (= Q121), S134 (= S172), G135 (= G173), G136 (= G174), E158 (= E196), H191 (≠ Q228)
- binding magnesium ion: S42 (= S79), Q81 (= Q121)
Sites not aligning to the query:
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
34% identity, 77% coverage: 51:261/275 of query aligns to 14:224/371 of 3puvA
- binding adenosine-5'-diphosphate: V17 (= V54), G38 (= G75), C39 (≠ A76), G40 (= G77), K41 (= K78), S42 (= S79), T43 (= T80), R128 (≠ Q166), A132 (≠ S170), S134 (= S172), Q137 (≠ E175)
- binding magnesium ion: S42 (= S79), Q81 (= Q121)
Sites not aligning to the query:
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
34% identity, 77% coverage: 51:261/275 of query aligns to 12:222/367 of 1q12A
- binding adenosine-5'-triphosphate: S35 (≠ N74), G36 (= G75), C37 (≠ A76), G38 (= G77), K39 (= K78), S40 (= S79), T41 (= T80), R126 (≠ Q166), A130 (≠ S170), S132 (= S172), G134 (= G174), Q135 (≠ E175)
Sites not aligning to the query:
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
30% identity, 76% coverage: 54:262/275 of query aligns to 16:228/240 of 4ymuJ
- binding adenosine-5'-triphosphate: V16 (= V54), S36 (≠ N74), G37 (= G75), S38 (≠ A76), G39 (= G77), K40 (= K78), S41 (= S79), T42 (= T80), E162 (= E196), H194 (≠ Q228)
- binding magnesium ion: S41 (= S79), E162 (= E196)
Sites not aligning to the query:
Query Sequence
>WP_089303491.1 NCBI__GCF_900188115.1:WP_089303491.1
MSTEEPAQQDRGESTAAPEGAAAADSGAAERSTGSEVILDAVEVVAGYVPEVNVLTGCSL
ELGEGELVGVIGPNGAGKSTLIKAMFGLVPVRGGSVRFRGADITGAQTHTLVQHGLGYVP
QRDNVFPALTVEENLRMGTFLKPREFERRFAWVTELFSRLAERRKQRAGSLSGGERQMLA
MGRTLMAEPEVLFLDEPSAGLSPANQDQVFGQVQRINDTGVSIVMVEQNARRCLQICDRG
YVLDQGRNAYSGAGHDLLHDPKVIEYYLGTLATVD
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory