SitesBLAST
Comparing WP_089322320.1 NCBI__GCF_900188395.1:WP_089322320.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3h0mA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
61% identity, 98% coverage: 3:480/487 of query aligns to 2:470/478 of 3h0mA
- active site: K72 (= K81), S147 (= S156), S148 (= S157), S166 (= S175), T168 (= T177), G169 (= G178), G170 (= G179), S171 (= S180), Q174 (= Q183)
- binding glutamine: M122 (= M131), G123 (= G132), D167 (= D176), T168 (= T177), G169 (= G178), G170 (= G179), S171 (= S180), F199 (= F208), Y302 (= Y311), R351 (= R360), D418 (= D427)
3h0lA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
61% identity, 98% coverage: 3:480/487 of query aligns to 2:470/478 of 3h0lA
- active site: K72 (= K81), S147 (= S156), S148 (= S157), S166 (= S175), T168 (= T177), G169 (= G178), G170 (= G179), S171 (= S180), Q174 (= Q183)
- binding asparagine: G123 (= G132), S147 (= S156), G169 (= G178), G170 (= G179), S171 (= S180), Y302 (= Y311), R351 (= R360), D418 (= D427)
2f2aA Structure of tRNA-dependent amidotransferase gatcab complexed with gln (see paper)
55% identity, 99% coverage: 1:483/487 of query aligns to 1:480/485 of 2f2aA
- active site: K79 (= K81), S154 (= S156), S155 (= S157), S173 (= S175), T175 (= T177), G176 (= G178), G177 (= G179), S178 (= S180), Q181 (= Q183)
- binding glutamine: G130 (= G132), S154 (= S156), D174 (= D176), T175 (= T177), G176 (= G178), S178 (= S180), F206 (= F208), Y309 (= Y311), Y310 (= Y312), R358 (= R360), D425 (= D427)
2dqnA Structure of tRNA-dependent amidotransferase gatcab complexed with asn (see paper)
55% identity, 99% coverage: 1:483/487 of query aligns to 1:480/485 of 2dqnA
- active site: K79 (= K81), S154 (= S156), S155 (= S157), S173 (= S175), T175 (= T177), G176 (= G178), G177 (= G179), S178 (= S180), Q181 (= Q183)
- binding asparagine: M129 (= M131), G130 (= G132), T175 (= T177), G176 (= G178), S178 (= S180), Y309 (= Y311), Y310 (= Y312), R358 (= R360), D425 (= D427)
3kfuE Crystal structure of the transamidosome (see paper)
45% identity, 96% coverage: 9:477/487 of query aligns to 3:455/468 of 3kfuE
4n0iA Crystal structure of s. Cerevisiae mitochondrial gatfab in complex with glutamine (see paper)
36% identity, 81% coverage: 73:468/487 of query aligns to 30:442/450 of 4n0iA
- active site: K38 (= K81), S116 (= S156), S117 (= S157), T135 (≠ S175), T137 (= T177), G138 (= G178), G139 (= G179), S140 (= S180), L143 (≠ Q183)
- binding glutamine: G89 (= G132), T137 (= T177), G138 (= G178), S140 (= S180), Y168 (≠ F208), Y271 (= Y311), Y272 (= Y312), R320 (= R360), D404 (= D427)
3a1iA Crystal structure of rhodococcus sp. N-771 amidase complexed with benzamide (see paper)
34% identity, 83% coverage: 73:477/487 of query aligns to 87:498/508 of 3a1iA
- active site: K95 (= K81), S170 (= S156), S171 (= S157), G189 (≠ S175), Q191 (≠ T177), G192 (= G178), G193 (= G179), A194 (≠ S180), I197 (≠ Q183)
- binding benzamide: F145 (≠ M131), S146 (≠ G132), G147 (≠ S133), Q191 (≠ T177), G192 (= G178), G193 (= G179), A194 (≠ S180), W327 (≠ Y311)
Q7XJJ7 Fatty acid amide hydrolase; AtFAAH; N-acylethanolamine amidohydrolase; EC 3.5.1.99 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
30% identity, 96% coverage: 18:485/487 of query aligns to 141:597/607 of Q7XJJ7
- K205 (= K81) mutation to A: Loss of activity.
- SS 281:282 (= SS 156:157) mutation to AA: Loss of activity.
- GGGS 302:305 (≠ TGGS 177:180) binding substrate
- S305 (= S180) mutation to A: Loss of activity.
- R307 (= R182) mutation to A: Loss of activity.
- S360 (≠ L235) mutation to A: No effect.
6diiH Structure of arabidopsis fatty acid amide hydrolase in complex with methyl linolenyl fluorophosphonate (see paper)
30% identity, 96% coverage: 18:485/487 of query aligns to 141:597/616 of 6diiH
- binding methyl-9Z,12Z,15Z-octadecatrienylphosphonofluoridate: G255 (≠ A130), T258 (≠ S133), S281 (= S156), G302 (≠ T177), G303 (= G178), S305 (= S180), S472 (≠ T365), I532 (vs. gap), M539 (≠ I421)
Sites not aligning to the query:
8ey9B Structure of arabidopsis fatty acid amide hydrolase mutant s305a in complex with 9-hydroxy-10,12-octadecadienoyl-ethanolamide
29% identity, 96% coverage: 18:485/487 of query aligns to 141:597/605 of 8ey9B