SitesBLAST
Comparing WP_089322763.1 NCBI__GCF_900188395.1:WP_089322763.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7byaA Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) complexed with oxaloacetic acid (oaa) and adenosine 5'- diphosphoribose (apr) (see paper)
56% identity, 99% coverage: 1:311/313 of query aligns to 2:310/311 of 7byaA
7by9A Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) complexed with oxaloacetic acid (oaa) and nicotinamide adenine dinucleotide (NAD) (see paper)
56% identity, 99% coverage: 1:311/313 of query aligns to 2:310/311 of 7by9A
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), G13 (= G12), F14 (≠ N13), T15 (≠ I14), D35 (= D34), I36 (= I35), T81 (= T78), A82 (= A79), I102 (= I99), Q105 (≠ S102), V106 (= V103), L122 (≠ V119), N124 (= N121), V126 (≠ L123)
- binding oxaloacetate ion: L151 (= L148), R155 (= R152), H179 (= H176), G217 (= G214)
7f8dA Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) g218y mutant (see paper)
55% identity, 99% coverage: 1:311/313 of query aligns to 4:312/313 of 7f8dA
- binding nicotinamide-adenine-dinucleotide: G15 (= G12), F16 (≠ N13), T17 (≠ I14), D37 (= D34), I38 (= I35), L41 (vs. gap), Y71 (= Y66), T83 (= T78), A84 (= A79), G85 (= G80), L124 (≠ V119), T125 (= T120), N126 (= N121), Q149 (≠ M144), L153 (= L148), P235 (= P230)
4pltB Crystal structure of ancestral apicomplexan malate dehydrogenase with oxamate. (see paper)
56% identity, 98% coverage: 2:309/313 of query aligns to 2:315/319 of 4pltB
- active site: R94 (= R89), D154 (= D149), R157 (= R152), H181 (= H176)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G12 (= G12), N13 (= N13), I14 (= I14), F33 (≠ I33), D34 (= D34), I35 (= I35), T78 (= T78), A79 (= A79), G80 (= G80), I81 (≠ F81), P82 (= P82), I104 (= I99), V124 (= V119), N126 (= N121), M149 (= M144), H181 (= H176), P240 (= P230)
4plyA Crystal structure of ancestral apicomplexan malate dehydrogenase with malate. (see paper)
56% identity, 98% coverage: 2:309/313 of query aligns to 1:314/318 of 4plyA
- active site: D153 (= D149), R156 (= R152), H180 (= H176)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G12), N12 (= N13), I13 (= I14), D33 (= D34), I34 (= I35), Y65 (= Y66), T77 (= T78), A78 (= A79), G79 (= G80), I80 (≠ F81), P81 (= P82), I103 (= I99), V123 (= V119), N125 (= N121), M148 (= M144), L152 (= L148), H180 (= H176), P239 (= P230)
- binding pyruvic acid: W91 (vs. gap), R93 (= R89), N125 (= N121), R156 (= R152), H180 (= H176), G223 (= G214)
P49814 Malate dehydrogenase; Vegetative protein 69; VEG69; EC 1.1.1.37 from Bacillus subtilis (strain 168) (see 2 papers)
53% identity, 99% coverage: 3:311/313 of query aligns to 5:311/312 of P49814
- S149 (≠ A145) modified: Phosphoserine
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
5ujkA Malate dehydrogenase from methylobacterium extorquens, complexed with NAD (see paper)
51% identity, 98% coverage: 3:309/313 of query aligns to 2:309/315 of 5ujkA
- active site: R88 (= R89), D148 (= D149), R151 (= R152), H175 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), Q12 (≠ N13), I13 (= I14), D33 (= D34), I34 (= I35), T77 (= T78), A78 (= A79), G79 (= G80), V98 (≠ I99), I118 (≠ V119), N120 (= N121), M143 (= M144), L147 (= L148), H175 (= H176)
4rosA Crystal structure of methylobacterium extorquens malate dehydrogenase complexed with oxaloacetate and adenosine-5-diphosphoribose
51% identity, 98% coverage: 3:309/313 of query aligns to 2:309/318 of 4rosA
- active site: R88 (= R89), D148 (= D149), R151 (= R152), H175 (= H176)
- binding adenosine-5-diphosphoribose: G11 (= G12), Q12 (≠ N13), I13 (= I14), D33 (= D34), I34 (= I35), T77 (= T78), A78 (= A79), G79 (= G80), V80 (≠ F81), P81 (= P82), V98 (≠ I99), T119 (= T120), N120 (= N121)
- binding oxaloacetate ion: R82 (= R83), R88 (= R89), N120 (= N121), L147 (= L148), R151 (= R152), H175 (= H176), G218 (= G214)
3gvhA Crystal structure of lactate/malate dehydrogenase from brucella melitensis
49% identity, 98% coverage: 3:309/313 of query aligns to 2:309/318 of 3gvhA
- active site: R88 (= R89), D148 (= D149), R151 (= R152), H175 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), I13 (= I14), D33 (= D34), I34 (= I35), T77 (= T78), A78 (= A79), I118 (≠ V119), N120 (= N121), M143 (= M144), L147 (= L148), H175 (= H176)
1uxgA Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface. (see paper)
51% identity, 99% coverage: 3:311/313 of query aligns to 1:305/308 of 1uxgA
- active site: R87 (= R89), D147 (= D149), R150 (= R152), H174 (= H176)
- binding fumaric acid: R81 (= R83), R87 (= R89), L146 (= L148), R150 (= R152), H174 (= H176), G212 (= G214), S223 (= S225)
- binding nicotinamide-adenine-dinucleotide: G10 (= G12), F11 (≠ N13), V12 (≠ I14), D32 (= D34), T76 (= T78), S77 (≠ A79), G78 (= G80), A79 (≠ F81), P80 (= P82), C101 (≠ V103), V117 (= V119), N119 (= N121), Q142 (≠ M144), H174 (= H176), P228 (= P230)
1guzA Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases (see paper)
50% identity, 96% coverage: 5:304/313 of query aligns to 2:298/305 of 1guzA
- active site: D147 (= D149), R150 (= R152), H174 (= H176)
- binding nicotinamide-adenine-dinucleotide: G9 (= G12), N10 (= N13), V11 (≠ I14), D32 (= D34), V33 (≠ I35), T76 (= T78), G78 (= G80), L79 (≠ F81), P80 (= P82), I97 (= I99), V117 (= V119), N119 (= N121), M142 (= M144), L146 (= L148), H174 (= H176), P228 (= P230)
P80040 Malate dehydrogenase; EC 1.1.1.37 from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) (see 2 papers)
50% identity, 99% coverage: 3:311/313 of query aligns to 2:306/309 of P80040
4plfB Crystal structure of ancestral apicomplexan lactate dehydrogenase with lactate. (see paper)
48% identity, 98% coverage: 2:307/313 of query aligns to 3:314/330 of 4plfB
- active site: R95 (= R89), D155 (= D149), R158 (= R152), H182 (= H176)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), M14 (≠ N13), I15 (= I14), F34 (≠ I33), D35 (= D34), V36 (≠ I35), T79 (= T78), A80 (= A79), G81 (= G80), L82 (≠ F81), T83 (≠ P82), I105 (= I99), I125 (≠ V119), N127 (= N121), M150 (= M144), H182 (= H176), P241 (= P230)
- binding pyruvic acid: W93 (vs. gap), R95 (= R89), N127 (= N121), R158 (= R152), H182 (= H176)
4plcA Crystal structure of ancestral apicomplexan lactate dehydrogenase with malate. (see paper)
48% identity, 98% coverage: 2:307/313 of query aligns to 3:314/324 of 4plcA