SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_089323376.1 NCBI__GCF_900188395.1:WP_089323376.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 17 hits to proteins with known functional sites (download)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
56% identity, 95% coverage: 5:164/169 of query aligns to 2:159/170 of Q58667

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Q58667

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R26 (≠ N29) mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
T27 (= T30) mutation to A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
56% identity, 95% coverage: 5:164/169 of query aligns to 2:159/167 of 2pkpA

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2pkpA

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binding zinc ion: D13 (= D16), C63 (= C66)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 76% coverage: 16:144/169 of query aligns to 554:698/758 of O14289

query
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O14289

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Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 78% coverage: 16:146/169 of query aligns to 18:152/198 of P9WK95

query
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P9WK95

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Sites not aligning to the query:

154 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 2 papers)
30% identity, 58% coverage: 27:124/169 of query aligns to 757:852/909 of P09339

query
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P09339

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
450 C→S: Loss of aconitase activity. It is glutamate auxotroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of inactive aconitase.
741 R→E: Same aconitase activity compared to the wild-type. It is glutamate prototroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of active aconitase.

P19414 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
39% identity, 40% coverage: 57:123/169 of query aligns to 655:723/778 of P19414

query
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P19414

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Sites not aligning to the query:

1:16 modified: transit peptide, Mitochondrion
604 R→K: Strongly diminishes the catalytic activity towards both known substrates, aconitate and homoaconitate.

D9X0I3 Aconitate hydratase A; ACN; Aconitase; EC 4.2.1.3 from Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) (see paper)
42% identity, 33% coverage: 57:111/169 of query aligns to 805:859/931 of D9X0I3

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D9X0I3

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Sites not aligning to the query:

125:129 mutation Missing: Retains 40% of aconitase activity. Improves RNA-binding ability.
538 C→A: Loss of aconitase activity. Cannot rescue the growth defect of a disruption mutant and results in only a slight increase in PTT production in the mutant. Shows weak IRE-binding activity.
763 R→E: Loss of aconitase activity and IRE-binding activity; when associated with E-767.
767 Q→E: Loss of aconitase activity and IRE-binding activity; when associated with E-763.

P39533 Homocitrate dehydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 39% coverage: 58:123/169 of query aligns to 661:728/789 of P39533

query
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P39533

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Sites not aligning to the query:

610 K→R: Reduces catalytic activity towards homoaconitate by 45% and increases the activity towards aconitate by a factor 116.

Q9SIB9 Aconitate hydratase 3, mitochondrial; Aconitase 3; mACO1; Citrate hydro-lyase 3; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
49% identity, 28% coverage: 57:103/169 of query aligns to 864:910/990 of Q9SIB9

query
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Q9SIB9

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Sites not aligning to the query:

91 modified: Phosphoserine

A0QX20 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
35% identity, 38% coverage: 57:120/169 of query aligns to 811:881/943 of A0QX20

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Sites not aligning to the query:

394 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5acnA Structure of activated aconitase. Formation of the (4fe-4s) cluster in the crystal (see paper)
43% identity, 28% coverage: 57:103/169 of query aligns to 631:677/754 of 5acnA

query
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I

K

N

K

I

Q

G

G

L

L

active site: S642 (= S68), R644 (= R70)
binding tricarballylic acid: R666 (= R92)

Sites not aligning to the query:

active site: 100, 101, 165, 447
binding fe3-s4 cluster: 145, 147, 167, 358, 421, 424, 446
binding tricarballylic acid: 198, 235

P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see 2 papers)
45% identity, 28% coverage: 57:103/169 of query aligns to 658:704/780 of P20004

query
sites
P20004

I

V

V

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S

S

S
|
S

R
|
R

E

E

H

H

A

A

P

A

I

L

A

E

I

P

K

R

A

H

A

L

G

G

V

G

S

R

A

A

V

I

I

I

A

T

K

K

S

S

F

F

A

A

R

R

I

I

F

H

Y

E

R

T

N

N

C

L

I

K

N

K

I

Q

G

G

L

L

SR 670:671 (= SR 69:70) binding substrate

Sites not aligning to the query:

99 binding substrate
192:194 binding substrate
385 binding [4Fe-4S] cluster
448 binding [4Fe-4S] cluster
451 binding [4Fe-4S] cluster
474 binding substrate
479 binding substrate
607 binding substrate

8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound (see paper)
32% identity, 52% coverage: 57:144/169 of query aligns to 630:735/753 of 8acnA

query
sites
8acnA

I

V

V

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

P

A

I

L

A

E

I

P

K

R

A

F

A

L

G

G

V

G

S

R

A

A

V

I

I

I

A

T

K

K

S

S

F

F

A

A

R

R

I

I

F

H

Y

E

R

T

N

N

C

L

I

K

N

K

I

Q

G

G

-

L

L

L

P

P

I

L

-

T

F

F

E

A

S

D

P

P

E

A

A

D

V

Y

E

N

G

K

I

I

E

H

E

P

G

V

D

D

I

K

V

L

E

T

V

I

N

Q

-

G

-

L

-

K

-

D

-

F

-

A

-

P

-

G

-

K

P

P

V

L

T

T

G

C

I

I

R

K

N

H

-

P

-

N

-

G

-

T

-

Q

-

E

-

T

I

I

T

L

K

L

N

N

T

H

E

T

F

F

Q

N

A

E

T

T

P

Q

I

I

active site: S641 (= S68), R643 (= R70)
binding nitroisocitric acid: S641 (= S68), S642 (= S69), R643 (= R70)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding nitroisocitric acid: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 164, 166, 356, 357, 420, 423, 424

1fghA Complex with 4-hydroxy-trans-aconitate (see paper)
32% identity, 52% coverage: 57:144/169 of query aligns to 630:735/753 of 1fghA

query
sites
1fghA

I

V

V

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

P

A

I

L

A

E

I

P

K

R

A

F

A

L

G

G

V

G

S

R

A

A

V

I

I

I

A

T

K

K

S

S

F

F

A

A

R

R

I

I

F

H

Y

E

R

T

N

N

C

L

I

K

N

K

I

Q

G

G

-

L

L

L

P

P

I

L

-

T

F

F

E

A

S

D

P

P

E

A

A

D

V

Y

E

N

G

K

I

I

E

H

E

P

G

V

D

D

I

K

V

L

E

T

V

I

N

Q

-

G

-

L

-

K

-

D

-

F

-

A

-

P

-

G

-

K

P

P

V

L

T

T

G

C

I

I

R

K

N

H

-

P

-

N

-

G

-

T

-

Q

-

E

-

T

I

I

T

L

K

L

N

N

T

H

E

T

F

F

Q

N

A

E

T

T

P

Q

I

I

active site: S641 (= S68), R643 (= R70)
binding 4-hydroxy-aconitate ion: S641 (= S68), S642 (= S69), R643 (= R70)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding 4-hydroxy-aconitate ion: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 164, 166, 356, 357, 420, 423, 424, 451

1amjA Steric and conformational features of the aconitase mechanism (see paper)
32% identity, 52% coverage: 57:144/169 of query aligns to 630:735/753 of 1amjA

query
sites
1amjA

I

V

V

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

S

S

R
|
R

E

E

H

H

A

S

P

A

I

L

A

E

I

P

K

R

A

F

A

L

G

G

V

G

S

R

A

A

V

I

I

I

A

T

K

K

S

S

F

F

A

A

R

R

I

I

F

H

Y

E

R

T

N

N

C

L

I

K

N

K

I

Q

G

G

-

L

L

L

P

P

I

L

-

T

F

F

E

A

S

D

P

P

E

A

A

D

V

Y

E

N

G

K

I

I

E

H

E

P

G

V

D

D

I

K

V

L

E

T

V

I

N

Q

-

G

-

L

-

K

-

D

-

F

-

A

-

P

-

G

-

K

P

P

V

L

T

T

G

C

I

I

R

K

N

H

-

P

-

N

-

G

-

T

-

Q

-

E

-

T

I

I

T

L

K

L

N

N

T

H

E

T

F

F

Q

N

A

E

T

T

P

Q

I

I

active site: S641 (= S68), R643 (= R70)
binding sulfate ion: R643 (= R70)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding iron/sulfur cluster: 144, 166, 357, 420, 423
binding sulfate ion: 71, 579

1amiA Steric and conformational features of the aconitase mechanism (see paper)
32% identity, 52% coverage: 57:144/169 of query aligns to 630:735/753 of 1amiA

query
sites
1amiA

I

V

V

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

P

A

I

L

A

E

I

P

K

R

A

F

A

L

G

G

V

G

S

R

A

A

V

I

I

I

A

T

K

K

S

S

F

F

A

A

R

R

I

I

F

H

Y

E

R

T

N

N

C

L

I

K

N

K

I

Q

G

G

-

L

L

L

P

P

I

L

-

T

F

F

E

A

S

D

P

P

E

A

A

D

V

Y

E

N

G

K

I

I

E

H

E

P

G

V

D

D

I

K

V

L

E

T

V

I

N

Q

-

G

-

L

-

K

-

D

-

F

-

A

-

P

-

G

-

K

P

P

V

L

T

T

G

C

I

I

R

K

N

H

-

P

-

N

-

G

-

T

-

Q

-

E

-

T

I

I

T

L

K

L

N

N

T

H

E

T

F

F

Q

N

A

E

T

T

P

Q

I

I

active site: S641 (= S68), R643 (= R70)
binding alpha-methylisocitric acid: S641 (= S68), S642 (= S69), R643 (= R70)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding alpha-methylisocitric acid: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 144, 164, 166, 356, 357, 420, 423, 445

1acoA Crystal structure of aconitase with transaconitate bound (see paper)
32% identity, 52% coverage: 57:144/169 of query aligns to 630:735/753 of 1acoA

query
sites
1acoA

I

V

V

I

A

G

G

D

K

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

H

H

A

S

P

A

I

L

A

E

I

P

K

R

A

F

A

L

G

G

V

G

S

R

A

A

V

I

I

I

A

T

K

K

S

S

F

F

A

A

R

R

I

I

F

H

Y

E

R

T

N

N

C

L

I

K

N

K

I

Q

G

G

-

L

L

L

P

P

I

L

-

T

F

F

E

A

S

D

P

P

E

A

A

D

V

Y

E

N

G

K

I

I

E

H

E

P

G

V

D

D

I

K

V

L

E

T

V

I

N

Q

-

G

-

L

-

K

-

D

-

F

-

A

-

P

-

G

-

K

P

P

V

L

T

T

G

C

I

I

R

K

N

H

-

P

-

N

-

G

-

T

-

Q

-

E

-

T

I

I

T

L

K

L

N

N

T

H

E

T

F

F

Q

N

A

E

T

T

P

Q

I

I

active site: S641 (= S68), R643 (= R70)
binding aconitate ion: S641 (= S68), S642 (= S69), R643 (= R70)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding iron/sulfur cluster: 100, 144, 164, 166, 356, 357, 420, 423, 445
binding aconitate ion: 71, 164, 165, 446, 451, 579

Query Sequence

>WP_089323376.1 NCBI__GCF_900188395.1:WP_089323376.1
MSSEVYRGKAFKFGDDINTDEIIPARYLNTSDPAELAKHCMEDADPEFPSKVQKGDIIVA
GKNFGCGSSREHAPIAIKAAGVSAVIAKSFARIFYRNCINIGLPIFESPEAVEGIEEGDI
VEVNPVTGIIRNITKNTEFQATPIPPEIREIMDAGGLMEYAKKKLKENR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory