SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_089752071.1 NCBI__GCF_900113125.1:WP_089752071.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
40% identity, 98% coverage: 5:517/526 of query aligns to 18:534/541 of Q5SKN9

query
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Q5SKN9

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T184 (= T170) binding Mg(2+)
G302 (= G285) binding tetradecanoyl-AMP
Q322 (= Q306) binding tetradecanoyl-AMP
G323 (≠ V307) binding tetradecanoyl-AMP
T327 (= T311) binding tetradecanoyl-AMP
E328 (= E312) binding Mg(2+)
D418 (= D401) binding tetradecanoyl-AMP
K435 (= K418) binding tetradecanoyl-AMP
K439 (≠ I422) binding tetradecanoyl-AMP

P0DX84 3-methylmercaptopropionyl-CoA ligase; MMPA-CoA ligase; EC 6.2.1.44 from Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) (Silicibacter lacuscaerulensis) (see paper)
35% identity, 98% coverage: 1:517/526 of query aligns to 6:533/539 of P0DX84

query
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P0DX84

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H231 (= H214) mutation to A: Retains 74% of wild-type activity.
W235 (= W218) mutation to A: Almost completely abolishes the activity.
G302 (≠ A284) mutation to P: Almost completely abolishes the activity.
G303 (= G285) mutation to P: Almost completely abolishes the activity.
W326 (≠ Y308) mutation to A: Retains 7.7% of wild-type activity.
P333 (= P315) mutation to A: Retains 69% of wild-type activity.
R432 (= R416) mutation to A: Retains 4.3% of wild-type activity.
K434 (= K418) mutation to A: Retains 36% of wild-type activity.
D435 (= D419) mutation to A: Retains 76% of wild-type activity.
K438 (≠ I422) mutation to A: Retains 5.6% of wild-type activity.
G440 (= G424) mutation to P: Retains 3.6% of wild-type activity.
G441 (= G425) mutation to P: Retains 2.7% of wild-type activity.
E442 (= E426) mutation to A: Retains 27% of wild-type activity.
W443 (≠ N427) mutation to A: Retains 60% of wild-type activity.
E474 (= E458) mutation to A: Retains 33% of wild-type activity.
K523 (= K507) Plays an important role in catalysis; mutation to A: Retains 1.6% of wild-type activity.; mutation to E: Retains 1.4% of wild-type activity.; mutation to R: Retains 57% of wild-type activity.
K526 (= K510) mutation to A: Retains 48% of wild-type activity.

6ijbB Structure of 3-methylmercaptopropionate coa ligase mutant k523a in complex with amp and mmpa (see paper)
34% identity, 98% coverage: 1:517/526 of query aligns to 6:533/538 of 6ijbB

query
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6ijbB

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A

-

S

E

F

F

A

Y

D

D

E

E

L

L

F

I

E

E

R

T

A

G

D

D

-

T

-

D

-

F

-

E

-

W

-

P

-

V

L

F

E

D

E

E

T

N

D

T

I

A

A

S

S

S

L

L

L

C

Y

Y

T
|
T

S

S

G

G

T

T

G

G

N

H

P

P

K

K

G

G

V

V

L

L

L

Y

S

S

H

H

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R

A

S

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Y

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H

-

S

-

F

A

A

M

S

S

N

T

T

M

R

H

D

H

V

L

I

R

G

V

Y

S

S

D

A

Q

M

D

D

T

V

L

V

L

M

H

P

V

V

L

V

P

P

M

M

F

F

H
|
H

V

V

N

N

G

A

W
|
W

G

G

S

S

P

P

F

Y

Y

G

Y

C

T

A

A

M

N

S

G

G

A

A

T

-

Q

Q

V

M

M

V

L

L

R

P

K

G

T

P

D

D

-

L

-

H

P

G

K

E

L

A

M

L

L

V

E

N

K

L

I

I

K

D

K

T

H

Y

G

G

V

V

S

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L

L

A

H

M

M

G

A

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P

P

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I

L

W

N

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L

L

M

L

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A

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L

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L

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R

R

V

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V

V

I

I

A

G

G
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G

S
x
A

A
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A

P

C

P

P

A

S

F

M

V

I

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A

K

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F

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L

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G

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K

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I

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Q
x
H

V
x
A

Y
x
W

G
|
G

M
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M

T
x
S

E
|
E

I

M

S

S

P

P

L

L

I

G

T

T

T

A

S

N

E

I

L

P

R

L

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A

M

K

D

H

L

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D

K

L

L

P

P

S

I

E

E

Q

Q

Y

H

R

K

L

L

K

R

A

E

K

N

A
x
Q

G

G

Y

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P

M

P

M

F

G

G

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V

K

E

V

L

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K

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I

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V

D

D

E

D

F

D

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G

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N

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D

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L

A

P

H

H

D

D

G

G

K

V

S

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I

Q

G

G

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D

I

L

I

M

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V

R

R

T

G

N

H

G

W

I

V

M

L

E

D

G

S

Y

Y

F

F

K

Q

N

L

E

K

E

D

A

-

T

-

N

Q

A

E

A

L

I

L

R

Q

D

D

G

G

W

W

L

F

Y

A

T

T

G

G

D
|
D

M

V

A

A

V

T

V

L

D

D

E

P

K

D

H

G

T

Y

I

M

E

T

I

I

V

R

D

D

R

R

K

S

K

K

D

D

V

I

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

I

V

E

E

V

L

E

E

A

N

V

I

L

A

Y

V

E

A

H

H

P

P

S

K

V

L

L

A

E

T

A

A

V

V

V

I

A

G

T

V

P

P

H

H

D

P

K

K

W

W

G

D

E

E

V

R

P

P

K

L

A

L

V

V

V

A

V

V

L

K

R

A

E

E

G

G

E

E

T

D

V

P

S

S

E

E

E

A

E

E

L

L

I

L

M

E

F

F

C

F

R

D

E

G

K

K

L

I

A

A

H

K

F

W

K

Q

A

V

P

P

N

D

S

K

I

V

T

V

F

F

I

V

D

D

E

A

L

L

P

P

K

L

T

N

A

A

S

T

G

G

K
x
A

I

V

Q

L

K

K

V

R

I

K

I

L

R

R

K

D

S

E

F

F

active site: T185 (= T170), H205 (= H190), H231 (= H214), S329 (≠ T311), E330 (= E312), K438 (≠ I422), W443 (≠ N427), A523 (≠ K507)
binding 3-(methylsulfanyl)propanoic acid: W235 (= W218), G303 (= G285), A325 (≠ V307), W326 (≠ Y308), G327 (= G309), M328 (= M310)
binding adenosine monophosphate: G303 (= G285), A304 (≠ S286), A305 (= A287), H324 (≠ Q306), W326 (≠ Y308), G327 (= G309), M328 (= M310), S329 (≠ T311), Q359 (≠ A341), D417 (= D401)

6ihkB Structure of mmpa coa ligase in complex with adp (see paper)
34% identity, 98% coverage: 1:517/526 of query aligns to 6:530/533 of 6ihkB

query
sites
6ihkB

M

M

Q

T

V

Q

P

P

L

L

V

L

L

I

T

S

D

S

F

L

L

I

D

D

R

H

A

A

V

A

E

R

L

Y

Y

H

G

G

D

Q

K

T

P

E

A

I

I

V

-

S

I

V

D

E

D

T

E

D

R

G

T

T

L

V

T

T

-

R

-

T

-

N

Y

W

K

G

Q

E

L

I

N

A

G

A

R

N

V

A

N

R

Q

R

L

M

S

G

R

S

G

A

L

L

S

T

D

K

L

L

G

G

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L

T

Q

K

P

E

Q

D

D

K

R

V

I

A

G

Y

T

L

L

A

A

P

W

N

N

T

N

L

R

E

R

M

H

L

L

E

E

G

I

F

Y

Y

Y

G

A

V

A

F

S

Q

G

V

A

G

G

A

F

V

V

M

C

T

H

P

T

L

I

N

N

T

P

R

R

L

L

K

F

P

P

A

E

D

Q

Y

L

Q

V

F

Y

I

I

L

I

E

N

H

H

S

A

E

Q

S

D

K

R

V

V

L

L

F

F

V

F

D

D

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A

D

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L

F

Y

L

H

P

L

L

V

V

E

A

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A

V

I

L

R

P

D

Q

Q

L

L

P

T

Q

E

L

V

E

K

H

H

V

F

I

V

V

L

H

M

G

G

D

P

E

R

K

N

E

E

G

D

A

A

T

L

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Q

Y

Q

E

I

P

P

W

G

L

L

A

-

S

E

F

F

A

Y

D

D

E

E

L

L

F

I

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E

R

T

A

G

D

D

-

T

-

D

-

F

-

E

-

W

-

P

-

V

L

F

E

D

E

E

T

N

D

T

I

A

A

S

S

S

L

L

L

C

Y

Y

T
|
T

S

S

G

G

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-

G

-

N

H

P

P

K

K

G

G

V

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Y

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H

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H

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F

A

A

M

S

S

N

T

T

M

R

H

D

H

V

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I

R

G

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S

D

A

Q

M

D

D

T

V

L

V

L

M

H

P

V

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L

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P

M

M

F

F

H
|
H

V

V

N

N

G

A

W

W

G

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S

P

P

F

Y

Y

G

Y

C

T

A

A

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G

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-

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Q

V

M

M

V

L

L

R

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K

G

T

P

D

D

-

L

-

H

P

G

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E

L

A

M

L

L

V

E

N

K

L

I

I

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D

K

T

H

Y

G

G

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V

S

T

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L

L

A

H

M

M

G

A

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N

Q

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E

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L

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E

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R

V

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V

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I

A

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x
A

A
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A

P

C

P

P

A

S

F

M

V

I

R

A

K

T

V

F

E

R

E

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D

K

L

Y

G

G

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K

D

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x
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M
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E

I

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S

P

P

L

L

I

G

T

T

T

A

S

N

E

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L

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A

M

K

D

H

L

R

D

K

L

L

P

P

S

I

E

E

Q

Q

Y

H

R

K

L

L

K

R

A

E

K

N

A
x
Q

G

G

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R

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M

P

M

F

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V

K

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L

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D

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D

F

D

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G

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N

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A

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H

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D

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G

K

V

S

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I

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G

G

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I

L

I

M

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R

R

T

G

N

H

G

W

I

V

M

L

E

D

G

S

Y

Y

F

F

K

Q

N

L

E

K

E

D

A

-

T

-

N

Q

A

E

A

L

I

L

R

Q

D

D

G

G

W

W

L

F

Y

A

T

T

G

G

D
|
D

M

V

A

A

V

T

V

L

D

D

E

P

K

D

H

G

T

Y

I

M

E

T

I

I

V

R

D

D

R
|
R

K

S

K

K

D

D

V

I

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

I

V

E

E

V

L

E

E

A

N

V

I

L

A

Y

V

E

A

H

H

P

P

S

K

V

L

L

A

E

T

A

A

V

V

V

I

A

G

T

V

P

P

H

H

D

P

K

K

W

W

G

D

E

E

V

R

P

P

K

L

A

L

V

V

V

A

V

V

L

K

R

A

E

E

G

G

E

E

T

D

V

P

S

S

E

E

E

A

E

E

L

L

I

L

M

E

F

F

C

F

R

D

E

G

K

K

L

I

A

A

H

K

F

W

K

Q

A

V

P

P

N

D

S

K

I

V

T

V

F

F

I

V

D

D

E

A

L

L

P

P

K

L

T

N

A

A

S

T

G

G

K
|
K

I

V

Q

L

K

K

V

R

I

K

I

L

R

R

K

D

S

E

F

F

active site: T185 (= T170), H202 (= H190), H228 (= H214), S326 (≠ T311), E327 (= E312), K435 (≠ I422), W440 (≠ N427), K520 (= K507)
binding adenosine-5'-diphosphate: H228 (= H214), G300 (= G285), A301 (≠ S286), A302 (= A287), H321 (≠ Q306), A322 (≠ V307), W323 (≠ Y308), G324 (= G309), M325 (= M310), S326 (≠ T311), Q356 (≠ A341), D414 (= D401), R429 (= R416), K520 (= K507)

1v26B Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
38% identity, 96% coverage: 5:509/526 of query aligns to 11:502/510 of 1v26B

query
sites
1v26B

L

L

V

N

L

L

T

W

D

D

F

F

L

L

D

E

R

R

A

A

V

A

E

A

L

L

Y

F

G

G

D

R

K

K

P

E

A

V

I

V

-

S

-

R

-

L

-

H

I

T

D

G

D

E

E

V

R

H

T

R

L

T

T

T

Y

Y

K

A

Q

E

L

V

N

Y

G

Q

R

R

V

A

N

R

Q

R

L

L

S

M

R

G

G

G

L

L

S

R

D

A

L

L

G

G

V

V

T

G

K

V

E

G

D

D

K

R

V

V

A

A

Y

T

L

L

A

G

P

F

N

N

T

H

L

F

E

R

M

H

L

L

E

E

G

A

F

Y

Y

F

G

A

V

V

F

P

Q

G

V

M

G

G

A

A

V

V

M

L

T

H

P

T

L

A

N

N

T

P

R

R

L

L

K

S

P

P

A

K

D

E

Y

I

Q

A

F

Y

I

I

L

L

E

N

H

H

S

A

E

E

S

D

K

K

V

V

L

L

F

L

V

F

D

D

S

P

D

N

L

L

Y

L

H

P

L

L

V

V

E

E

T

A

V

I

L

R

P

G

Q

E

L

L

P

K

Q

T

L

V

E

Q

H

H

V

F

I

V

V

V

H

M

G

-

D

D

E

E

K

K

-

A

-

P

E

E

G

G

A

Y

T

L

S

A

Y

Y

E

E

P

E

W

A

L

L

A

G

S

E

F

E

A

A

D

D

E

P

L

V

F

-

E

-

R

-

A

-

D

R

L

V

E

P

E

E

T

R

D

A

I

A

A

C

S

G

L

M

L

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

N

L

P

P

K

K

G

G

V

V

L

V

L

Y

S

S

H

H

R

R

A

A

N

L

Y

V

L

L

H
|
H

-

S

-

L

A

A

M

A

S

S

T

L

M

V

H

D

H

G

L

T

R

A

V

L

S

S

D

E

Q

K

D

D

T

V

L

V

L

L

H

P

V

V

L

V

P

P

M

M

F

F

H
|
H

V

V

N

N

G

A

W

W

G

C

S

L

P

P

F

Y

Y

A

T

T

A

L

N

V

G

G

A

A

T

K

Q

Q

V

V

M

L

L

P

R

G

-

P

K

R

T

L

D

D

P

P

K

A

L

S

M

L

L

V

E

E

K

L

I

F

K

D

K

G

H

E

G

G

V

V

S

T

V

F

L

T

H

A

M

G

A

V

P

P

T

T

V

V

L

W

N

L

M

A

L

L

M

A

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D

E

Y

Y

L

D

E

A

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T

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G

P

H

Q

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I

L

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K

Q

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D

L

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R

R

R

V

L

V

V

I

V

A

G

G
|
G

S
|
S

A
|
A

P

A

P

P

P

R

A

S

F

L

V

I

R

A

K

R

V

F

E

E

E

R

D

-

L

M

G

G

W

V

K

E

F

V

I

R

Q

Q

V
x
G

Y
|
Y

G
|
G

M
x
L

T
|
T

E
|
E

I

T

S

S

P

P

L

V

I

V

T

V

T

Q

S

N

E

F

L

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K

S

S

M

H

D

L

L

E

D

S

L

L

P

S

S

E

E

E

Q

K

Y

L

R

T

L

L

K

K

A

A

K

K

A

T

G

G

Y

L

E

P

M

I

M

P

G

L

S

V

K

R

V

L

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R

V

V

V

A

D

D

E

E

F

E

G

G

E

R

E

P

V

V

A

P

H

K

D

D

G

G

K

K

S

A

I

L

G

G

E

E

I

V

I

Q

T

L

R

K

T

G

N

P

G

W

I

I

M

T

E

G

G

G

Y

Y

F

Y

K

G

N

N

E

E

A

A

T

T

N

R

A

S

A

A

I

L

R

T

-

P

D

D

G

G

W

F

L

F

Y

R

T

T

G

G

D
|
D

M

I

A

A

V

V

V

W

D

D

E

E

K

E

H

G

T

Y

I

V

E

E

I

I

V

K

D

D

R

R

K

L

K
|
K

D

D

V

L

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

I

V

E

D

V

L

E

E

A

N

V

A

L

-

Y

-

E

-

H

-

P

-

S

-

V

-

L

-

E

-

A

A

V

V

A

A

T

I

P

P

H

H

D

P

K

K

W

W

G

Q

E

E

V

R

P

P

K

L

A

A

V

V

V

-

L

-

R

-

E

-

G

-

E

-

T

-

V

T

S

P

E

E

E

L

L

N

I

E

M

H

F

L

C

L

R

K

E

A

K

G

L

F

A

A

H

K

F

W

K

Q

A

L

P

P

N

D

S

A

I

Y

T

V

F

F

I

A

D

E

E

E

L

I

P

P

K

R

T

T

A

R

S

A

G

L

K

R

I

E

Q

Q

active site: T177 (= T170), H197 (= H190), H223 (= H214), T320 (= T311), E321 (= E312), K432 (≠ I422), W437 (≠ N427)
binding adenosine monophosphate: G295 (= G285), S296 (= S286), A297 (= A287), G316 (≠ V307), Y317 (= Y308), G318 (= G309), L319 (≠ M310), T320 (= T311), D411 (= D401), K428 (= K418), K432 (≠ I422), W437 (≠ N427)
binding magnesium ion: T177 (= T170), E321 (= E312)

1v25A Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
40% identity, 87% coverage: 5:464/526 of query aligns to 11:464/491 of 1v25A

query
sites
1v25A

L

L

V

N

L

L

T

W

D

D

F

F

L

L

D

E

R

R

A

A

V

A

E

A

L

L

Y

F

G

G

D

R

K

K

P

E

A

V

I

V

-

S

-

R

-

L

-

H

I

T

D

G

D

E

E

V

R

H

T

R

L

T

T

T

Y

Y

K

A

Q

E

L

V

N

Y

G

Q

R

R

V

A

N

R

Q

R

L

L

S

M

R

G

G

G

L

L

S

R

D

A

L

L

G

G

V

V

T

G

K

V

E

G

D

D

K

R

V

V

A

A

Y

T

L

L

A

G

P

F

N

N

T

H

L

F

E

R

M

H

L

L

E

E

G

A

F

Y

Y

F

G

A

V

V

F

P

Q

G

V

M

G

G

A

A

V

V

M

L

T

H

P

T

L

A

N

N

T

P

R

R

L

L

K

S

P

P

A

K

D

E

Y

I

Q

A

F

Y

I

I

L

L

E

N

H

H

S

A

E

E

S

D

K

K

V

V

L

L

F

L

V

F

D

D

S

P

D

N

L

L

Y

L

H

P

L

L

V

V

E

E

T

A

V

I

L

R

P

G

Q

E

L

L

P

K

Q

T

L

V

E

Q

H

H

V

F

I

V

V

V

H

M

G

-

D

D

E

E

K

K

-

A

-

P

E

E

G

G

A

Y

T

L

S

A

Y

Y

E

E

P

E

W

A

L

L

A

G

S

E

F

E

A

A

D

D

E

P

L

V

F

-

E

-

R

-

A

-

D

R

L

V

E

P

E

E

T

R

D

A

I

A

A

C

S

G

L

M

L

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

N

L

P

P

K

K

G

G

V

V

L

V

L

Y

S

S

H

H

R

R

A

A

N

L

Y

V

L

L

H
|
H

-

S

-

L

A

A

M

A

S

S

T

L

M

V

H

D

H

G

L

T

R

A

V

L

S

S

D

E

Q

K

D

D

T

V

L

V

L

L

H

P

V

V

L

V

P

P

M

M

F

F

H
|
H

V
|
V

N

N

G

A

W

W

G

C

S

L

P

P

F

Y

Y

A

T

T

A

L

N

V

G

G

A

A

T

K

Q

Q

V

V

M

L

L

P

R

G

-

P

K

R

T

L

D

D

P

P

K

A

L

S

M

L

L

V

E

E

K

L

I

F

K

D

K

G

H

E

G

G

V

V

S

T

V

F

L

T

H

A

M

G

A

V

P

P

T

T

V

V

L

W

N

L

M

A

L

L

M

A

E

D

E

Y

Y

L

D

E

A

S

V

T

Q

G

P

H

Q

R

I

L

E

K

Q

T

D

L

V

R

R

R

V

L

V

V

I

V

A

G

G
|
G

S
|
S

A
|
A

P

A

P

P

P

R

A

S

F

L

V

I

R

A

K

R

V

F

E

E

E

R

D

-

L

M

G

G

W

V

K

E

F

V

I

R

Q

Q

V

G

Y
|
Y

G
|
G

M
x
L

T
|
T

E
|
E

I

T

S

S

P

P

L

V

I

V

T

V

T

Q

S

N

E

F

L

V

R

K

S

S

M

H

D

L

L

E

D

S

L

L

P

S

S

E

E

E

Q

K

Y

L

R

T

L

L

K

K

A

A

K

K

A

T

G

G

Y

L

E

P

M

I

M

P

G

L

S

V

K

R

V

L

R

R

V

V

V

A

D

D

E

E

F

E

G

G

E

R

E

P

V

V

A

P

H

K

D

D

G

G

K

K

S

A

I

L

G

G

E

E

I

V

I

Q

T

L

R

K

T

G

N

P

G

W

I

I

M

T

E

G

G

G

Y

Y

F

Y

K

G

N

N

E

E

A

A

T

T

N

R

A

S

A

A

I

L

R

T

-

P

D

D

G

G

W

F

L

F

Y

R

T

T

G

G

D
|
D

M

I

A

A

V

V

V

W

D

D

E

E

K

E

H

G

T

Y

I

V

E

E

I
|
I

V

K

D

D

R

R

K

L

K

K

D

D

V

L

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

I

V

E

D

V

L

E

E

A

-

V

-

L

-

Y

-

E

-

H

-

P

-

S

-

V

-

L

-

E

N

A

A

V

V

A

A

T

I

P

P

H

H

D

P

K

K

W

W

G

Q

E

E

V

R

P

P

K

L

A

A

V

V

active site: T177 (= T170), H197 (= H190), H223 (= H214), T320 (= T311), E321 (= E312), K432 (≠ I422), W437 (≠ N427)
binding phosphoaminophosphonic acid-adenylate ester: H223 (= H214), V224 (= V215), G295 (= G285), S296 (= S286), A297 (= A287), Y317 (= Y308), G318 (= G309), L319 (≠ M310), T320 (= T311), D411 (= D401), I423 (= I413), K432 (≠ I422), W437 (≠ N427)
binding magnesium ion: T177 (= T170), E321 (= E312)

8i3iA Acyl-acp synthetase structure bound to amp-pnp in the presence of mgcl2
31% identity, 97% coverage: 5:515/526 of query aligns to 10:519/522 of 8i3iA

query
sites
8i3iA

L

L

V

L

L

I

T

K

D

N

F

L

L

L

D

F

R

S

A

P

V

V

E

A

L

F

Y

N

G

P

D

E

K

Q

P

E

A

I

I

V

I

Y

D

A

D

N

E

H

R

R

T

R

L

H

T

S

Y

Y

K

K

Q

T

L

F

N

H

G

D

R

R

V

V

N

R

Q

Q

L

F

S

A

R

N

G

A

L

L

S

T

D

K

L

M

G

G

V

V

T

K

K

K

E

G

D

D

K

T

V

V

A

A

Y

V

L

M

A

D

P

Y

N

D

T

S

L

H

E

R

M

Y

L

L

E

E

G

C

F

Y

Y

F

G

A

V

I

F

P

Q

M

V

I

G

G

A

A

V

K

M

L

T

H

P

M

L

I

N

N

T

V

R

R

L

L

K

S

P

P

A

E

D

Q

Y

I

Q

L

F

Y

I

T

L

I

E

D

H

H

S

A

E

E

S

D

K

D

V

I

L

I

F

L

V

I

D

H

S

E

D

E

L

F

Y

L

H

P

L

I

V

L

E

D

T

Q

V

I

L

K

P

G

Q

R

L

I

P

D

Q

T

L

V

E

T

H

R

V

Y

I

V

V

V

H

L

G

R

D

D

E

D

K

E

E

E

G

-

A

-

T

C

S

E

Y

Y

E

E

P

R

W

L

L

L

A

E

S

Q

F

E

A

S

D

T

E

E

L

-

F

Y

E

N

R

F

A

P

D

D

L

F

E

D

E

E

T

N

D

T

I

V

A

A

S

T

L

T

L

F

Y

Y

T
|
T

S

T

G
|
G

T
|
T

G

G

N

F

P

P

K
|
K

G

G

V

V

L

F

S

T

H

H

R

R

A

Q

N

L

Y

V

L

L

H

H

A

T

M

M

S

G

T

I

M

L

H

S

H

T

L

I

-

G

-

T

-

N

-

A

-

S

-

Q

-

G

R

R

V

L

S

H

D

Q

Q

G

D

D

T

I

L

Y

L

M

H

P

V

I

L

T

P

P

M

M

F

F

H

H

V

V

N

H

G

A

W

W

G

G

S

L

P

P

F

Y

Y

M

Y

A

T

T

A

M

N

L

G

G

A

V

T

K

Q

Q

V

V

M

Y

L

P

R

G

K

K

T

Y

D

V

P

P

K

D

L

V

M

L

L

L

E

N

K

L

I

I

K

E

K

Q

H

E

G

K

V

V

S

T

V

F

L

S

H

H

M

C

A

V

P

P

T

T

V

I

L

L

N

H

M

L

L

L

M

L

E

S

Y

P

D

K

A

S

V

K

Q

A

P

M

Q

D

I

F

E

-

Q

S

D

G

V

W

R

K

V

V

I

I

A

G

G
|
G

S
x
A

A
|
A

P

L

P

P

P

K

A

A

F

L

V

C

R

K

K

S

-

A

V

L

E

E

E

R

D

D

L

I

G

-

W

-

K

D

F

V

I

F

Q

A

V

G

Y
|
Y

G

G

M
|
M

T
x
S

E

E

I

T

S

G

P

P

L

I

I

L

T

S

T

I

S

V

E

Q

L

L

R

T

S

P

M

E

D

Q

L

L

D

E

L

L

P

D

S

V

E

D

E

Q

Q

Q

Y

A

R

E

L

Y

K

R

A

S

K

K

A

T

G

G

Y

K

E

K

M

V

M

A

G

L

S

V

K

E

V

A

R

Y

V

I

V

V

D

D

E

E

F

D

G

M

E

N

E

K

V

L

A

P

H

H

D

D

G

G

K

E

S

T

I

A

G

G

E

E

I

I

I

V

T

V

R

R

T

A

N

P

G

W

I

L

M

T

E

P

G

N

Y

Y

F

Y

K

K

N

D

E

N

E

K

A

N

T

S

N

K

A

A

A

L

I

W

R

R

D

G

G

G

W

Y

L

L

Y

H

T

T

G

G

D
|
D

M

V

A

A

V

H

V

I

D

D

E

D

K

E

H

G

T

F

I

I

E

K

I

I

V

T

D

D

R
|
R

K

V

K

K

D

D

V

M

I

I

I

K

S

I

G

S

G

G

E

E

N

W

I

V

S

S

I

L

E

E

V

L

E

E

A

D

V

I

L

L

Y

H

E

Q

H

H

P

Q

S

S

V

V

L

S

E

E

A

V

A

A

V

V

V

I

A

G

T

M

P

P

H

H

D

N

K

K

W

W

G

G

E

E

V

V

P

P

K

L

A

A

V

L

V

V

V

T

L

L

R

K

E

E

G

D

E

A

T

Q

V

V

S

T

E

E

E

K

E

E

L

L

I

L

M

G

F

F

C

A

R

K

E

D

K

R

L

E

A

A

H

L

F

L

K

L

A

-

P

-

N

-

S

K

I

V

T

K

F

I

I

V

D

D

E

E

L

I

P

A

K

K

T

T

A

S

S

V

G

G

K

K

I

V

Q

D

K

K

V

K

I

E

I

L

R

R

K

K

binding phosphoaminophosphonic acid-adenylate ester: T172 (= T170), G174 (= G172), T175 (= T173), T176 (= T174), K180 (= K178), G293 (= G285), A294 (≠ S286), A295 (= A287), Y315 (= Y308), M317 (= M310), S318 (≠ T311), D408 (= D401), R423 (= R416)

8jylA Acyl-acp synthetase structure bound to c10-ams
31% identity, 97% coverage: 5:515/526 of query aligns to 8:525/527 of 8jylA

query
sites
8jylA

L

L

V

L

L

I

T

K

D

N

F

L

L

L

D

F

R

S

A

P

V

V

E

A

L

F

Y

N

G

P

D

E

K

Q

P

E

A

I

I

V

I

Y

D

A

D

N

E

H

R

R

T

R

L

H

T

S

Y

Y

K

K

Q

T

L

F

N

H

G

D

R

R

V

V

N

R

Q

Q

L

F

S

A

R

N

G

A

L

L

S

T

D

K

L

M

G

G

V

V

T

K

K

K

E

G

D

D

K

T

V

V

A

A

Y

V

L

M

A

D

P

Y

N

D

T

S

L

H

E

R

M

Y

L

L

E

E

G

C

F

Y

Y

F

G

A

V

I

F

P

Q

M

V

I

G

G

A

A

V

K

M

L

T

H

P

M

L

I

N

N

T

V

R

R

L

L

K

S

P

P

A

E

D

Q

Y

I

Q

L

F

Y

I

T

L

I

E

D

H

H

S

A

E

E

S

D

K

D

V

I

L

I

F

L

V

I

D

H

S

E

D

E

L

F

Y

L

H

P

L

I

V

L

E

D

T

Q

V

I

L

K

P

G

Q

R

L

I

P

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binding magnesium ion: S316 (≠ T311), E317 (= E312)
binding [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl N-decanoylsulfamate: W225 (= W218), G290 (≠ A284), G291 (= G285), A292 (≠ S286), A293 (= A287), G312 (≠ V307), Y313 (= Y308), G314 (= G309), M315 (= M310), S316 (≠ T311), I321 (≠ L316), D406 (= D401), R421 (= R416), K427 (≠ I422), W432 (≠ N427)

8i6mA Acyl-acp synthetase structure bound to amp-c18:1
31% identity, 97% coverage: 5:515/526 of query aligns to 8:525/528 of 8i6mA

query
sites
8i6mA

L

L

V

L

L

I

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K

D

N

F

L

L

L

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