SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_090214906.1 NCBI__GCF_002796795.1:WP_090214906.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

9ggjF Crystal structure of argininosuccinate lyase from arabidopsis thaliana (atasl) in complex with biological substrate and products - argininosuccinate, argnine and fumarate (see paper)
49% identity, 97% coverage: 11:458/464 of query aligns to 1:447/454 of 9ggjF

query
sites
9ggjF

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binding arginine: R104 (= R114), N105 (= N115), Y312 (= Y322), Q317 (= Q327), K320 (= K330)
binding argininosuccinate: T150 (= T160), H151 (= H161)

9ggjA Crystal structure of argininosuccinate lyase from arabidopsis thaliana (atasl) in complex with biological substrate and products - argininosuccinate, argnine and fumarate (see paper)
49% identity, 94% coverage: 22:458/464 of query aligns to 7:442/451 of 9ggjA

query
sites
9ggjA

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binding argininosuccinate: N100 (= N115), V103 (= V118), Y307 (= Y322), Q312 (= Q327), K315 (= K330)
binding fumaric acid: T145 (= T160), H146 (= H161)

2e9fB Crystal structure of t.Th.Hb8 argininosuccinate lyase complexed with l-arginine
51% identity, 95% coverage: 17:459/464 of query aligns to 1:444/450 of 2e9fB

query
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2e9fB

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F

T

A

E

E

D

D

I

A

F

L

T

P

V

L

L

L

G

R

V

L

E

E

N

T

S

A

V

I

N

H

S

R

R

R

T

R

S

S

Y

Y

G

G

T

T

A

A

P

P

E

E

Q

A

V

V

R

R

A

E

Q

R

V

L

T

E

R

E

W

A

K

K

active site: E71 (= E87), T146 (= T160), H147 (= H161), S268 (= S282), S269 (= S283), K274 (= K288), E281 (= E295)
binding arginine: R98 (= R114), N99 (= N115), V102 (= V118), Y308 (= Y322), Q313 (= Q327), K316 (= K330)

P24058 Argininosuccinate lyase; ASAL; Arginosuccinase; Delta crystallin II; Delta-2 crystallin; EC 4.3.2.1 from Anas platyrhynchos (Mallard) (Anas boschas) (see 4 papers)
45% identity, 99% coverage: 7:464/464 of query aligns to 8:463/468 of P24058

query
sites
P24058

N

D

Q

K

M

L

W
|
W

G

G

R

R

F

F

A

S

A

G

G

S

P

T

D

D

A

P

I

I

M

M

E

E

A

K

I

L

N

N

A

S

S
|
S

I

I

G

A

F

Y

D
|
D

K

Q

R

R

M

L

A

S

A

E

Q

V

D

D

I

I

Q

Q

G

G

S

S

R

M

A

A

H

Y

A

A

A

K

M

A

L

L

A

E

A

K

S

A

G

G

V

I

I

L

T

T

D

K

S

T

D

E

A

L

E

E

A

K

I

I

R

L

E

S

G

G

L

L

L

E

T

K

I

I

L

S

S

E

E

E

I

W

E

S

A

K

G

G

N

V

F

F

Q

V

F

V

S

K

T

Q

A

S

L

D

E

E

D
|
D

I

I

H

H

M

T

N

A

V

N

E

E

A

R

R

R

L

L

K

K

E

E

I

L

I

I

G

G

E

D

P

I

A

A

G

G

R

K

L

L

H

H

T

T

G

G

R

R

S

S

R

R

N
|
N

D
|
D

Q

Q

V

V

A

V

T

T

D

D

F

L

K

K

L

L

W

F

V

M

R

K

D

N

Q

S

L

L

D

S

A

I

E

S

T

T

G

H

L

L

A

Q

L

L

I

I

A

K

A

T

L

L

L

V

D

E

Q

R

A

A

E

A

A

I

G

E

A

I

D

D

W

V

V

I

M

L

P

P

G

G

F

Y

T
|
T

H
|
H

L

L

Q

Q

T

K

A

A

Q

Q

P

P

V

I

T

R

W

W

G

S

H

Q

H

F

M

L

A

S

Y

H

V

A

E

V

M

A

F

L

G

T

R

R

D

D

L

S

S

E

R

R

V

L

R

G

D

E

A

V

R

K

T

K

R

R

M

I

N

N

E

V

C

L

P

P

L

L

G

G

A

S

A

G

A

A

L

L

A

A

G

G

T

N

S

P

F

L

P

D

I

I

D

D

R

R

S

E

M

M

T

L

A

R

E

S

A

E

L

L

G

E

F

F

E

A

R

S

P

I

A

S

A

L

N

N

S

S

L

M

D

D

A

A

V

I

S

S

D

E

R
|
R

D

D

F

F

A

V

L

V

E

E

F

F

L

L

S

S

V

F

A

A

S

T

I

L

C

L

A

M

V

I

H

H

L

L

S

S

R

K

F

M

A

A

E

E

E

D

L

L

V

I

I

I

W

Y

S

S

S

T

A

S

Q

E

F

F

R

G

F

F

V

L

T

T

L

L

S

S

D

D

R

A

F

F

S

S

T
|
T

G

G

S
|
S

S

S

I

L

M

M

P

P

Q

Q

K

K

N
|
N

P

P

D
|
D

A

S

A

L

E
|
E

L

L

I

I

R

R

A

S

K

K

V

A

G

G

R

R

I

V

F

F

G

G

A

R

N

L

T

A

A

S

L

I

M

L

M

M

V

V

M

L

K

K

G

G

L

L

P

P

L

S

A

T

Y
|
Y

S

N

K
|
K

D

D

M

L

Q
|
Q

E

E

D
|
D

K
|
K

E

E

Q

A

V

V

F

F

D

D

A

V

D

D

N

T

L

L

M

T

L

A

A

V

L

L

A

Q

A

V

M

A

E

T

G

G

M

V

V

I

K

S

D

T

M

L

S

Q

A

I

N

S

R

K

E

E

S

N

L

M

A

E

A

K

A

A

A

L

G

T

S

P

G

E

F

M

S

-

T

L

A

A

T

T

D

D

L

L

A

A

D

L

W

Y

L

L

V

V

R

R

V

K

L

-

K

G

V

V

P

P

F

F

R

R

D

Q

A

A

H

H

H

T

V

A

T

S

G

G

T

K

L

A

V

V

G

H

M

L

A

A

E

E

A

T

R

K

G

G

C

I

D

T

L

I

P

N

D

K

L

L

S

S

L

L

D

E

D

D

M

L

Q

K

G

S

V

I

H

S

S

P

G

Q

I

F

T

S

E

S

D

D

I

V

F

S

T

Q

V

V

L

F

G

N

V

F

E

V

N

N

S

S

V

V

N

E

S

Q

R

Y

T

T

S

A

Y

L

G

A

G

G

T

T

A

A

P

K

E

S

Q

S

V

V

R

T

A

T

Q

Q

V

I

T

E

R

Q

W

L

K

R

A

E

L

L

L

M

A

K

Q

K

W11 (= W10) mutation to A: 98% decrease in catalytic efficiency.; mutation to F: 90% decrease in catalytic efficiency.; mutation to M: 99% decrease in catalytic efficiency.; mutation to R: 97% decrease in catalytic efficiency.; mutation to Y: 50% decrease in catalytic efficiency.
S29 (= S28) binding in chain A; mutation to A: 10% decrease in catalytic efficiency.
D33 (= D32) mutation to N: 99% decrease in catalytic efficiency.
D89 (= D88) mutation to N: Loss of activity.
N116 (= N115) binding in chain A; mutation to D: 99% decrease in catalytic efficiency.
D117 (= D116) mutation to A: 55% decrease in catalytic efficiency.; mutation to E: 58% decrease in catalytic efficiency.
T161 (= T160) binding in chain C; mutation to A: Loss of activity.; mutation to D: Loss of activity.; mutation to S: 30% decrease in catalytic efficiency.; mutation to V: Loss of activity.
H162 (= H161) mutation to E: Loss of activity.
R238 (= R237) mutation to Q: Loss of activity.
T281 (= T280) mutation to V: 80% decrease in catalytic efficiency.
S283 (= S282) mutation to A: Loss of activity.; mutation to C: Loss of activity.; mutation to D: Loss of activity.; mutation to H: Loss of activity.; mutation to T: Loss of activity.
N291 (= N290) binding in chain B; mutation to L: Loss of activity.
D293 (= D292) mutation to N: 99% decrease in catalytic efficiency.
E296 (= E295) mutation to D: Loss of activity.
Y323 (= Y322) binding in chain A
K325 (= K324) mutation to N: 99% decrease in catalytic efficiency.
Q328 (= Q327) binding in chain A
D330 (= D329) mutation to N: Loss of activity.
K331 (= K330) binding in chain A; mutation to Q: Loss of activity.

1k7wD Crystal structure of s283a duck delta 2 crystallin mutant (see paper)
45% identity, 96% coverage: 19:464/464 of query aligns to 3:446/450 of 1k7wD

query
sites
1k7wD

D

D

A

P

I

I

M

M

E

E

A

K

I

L

N

N

A

S

S

S

I

I

G

A

F

Y

D

D

K

Q

R

R

M

L

A

S

A

E

Q

V

D

D

I

I

Q

Q

G

G

S

S

R

M

A

A

H

Y

A

A

A

K

M

A

L

L

A

E

A

K

S

A

G

G

V

I

I

L

T

T

D

K

S

T

D

E

A

L

E

E

A

K

I

I

R

L

E

S

G

G

L

L

L

E

T

K

I

I

L

S

S

E

E

E

I

W

E

S

A

K

G

G

N

V

F

F

Q

V

F

V

S

K

T

Q

A

S

L

D

E
|
E

D

D

I

I

H

H

M

T

N

A

V

N

E

E

A

R

R

R

L

L

K

K

E

E

I

L

I

I

G

G

E

D

P

I

A

A

G

G

R

K

L

L

H

H

T

T

G

G

R

R

S

S

R
|
R

N
|
N

D

D

Q

Q

V
|
V

A

V

T

T

D

D

F

L

K

K

L

L

W

F

V

M

R

K

D

N

Q

S

L

L

D

S

A

I

E

S

T

T

G

H

L

L

A

Q

L

L

I

I

A

K

A

T

L

L

L

V

D

E

Q

R

A

A

E

A

A

I

G

E

A

I

D

D

W

V

V

I

M

L

P

P

G

G

F

Y

T
|
T

H
|
H

L

L

Q

Q

T

K

A

A

Q

Q

P

P

V

I

T

R

W

W

G

S

H

Q

H

F

M

L

A

S

Y

H

V

A

E

V

M

A

F

L

G

T

R

R

D

D

L

S

S

E

R

R

V

L

R

G

D

E

A

V

R

K

T

K

R

R

M

I

N

N

E

V

C

L

P

P

L

L

G

G

A

S

A

G

A

A

L

L

A

A

G

G

T

N

S

P

F

L

P

D

I

I

D

D

R

R

S

E

M

M

T

L

A

R

E

S

A

E

L

L

G

E

F

F

E

A

R

S

P

I

A

S

A

L

N

N

S

S

L

M

D

D

A

A

V

I

S

S

D

E

R

R

D

D

F

F

A

V

L

V

E

E

F

F

L

L

S

S

V

F

A

A

S

T

I

L

C

L

A

M

V

I

H

H

L

L

S

S

R

K

F

M

A

A

E

E

E

D

L

L

V

I

I

I

W

Y

S

S

S

T

A

S

Q

E

F

F

R

G

F

F

V

L

T

T

L

L

S

S

D

D

R

A

F

F

S

S

T

T

G

G

S
x
A

S
|
S

I

L

M

M

P

P

Q

Q

K
|
K

K

K

N

N

P

P

D

D

A

S

A

L

E
|
E

L

L

I

I

R

R

A

S

K

K

V

A

G

G

R

R

I

V

F

F

G

G

A

R

N

L

T

A

A

S

L

I

M

L

M

M

V

V

M

L

K

K

G

G

L

L

P

P

L

S

A

T

Y
|
Y

S

N

K

K

D

D

M

L

Q
|
Q

E

E

D

D

K
|
K

E

E

Q

A

V

V

F

F

D

D

A

V

D

D

N

T

L

L

M

T

L

A

A

V

L

L

A

Q

A

V

M

A

E

T

G

G

M

V

V

I

K

S

D

T

M

L

S

Q

A

I

N

S

R

K

E

E

S

N

L

M

A

E

A

K

A

A

A

L

G

T

S

P

G

E

F

M

S

-

T

L

A

A

T

T

D

D

L

L

A

A

D

L

W

Y

L

L

V

V

R

R

V

K

L

-

K

G

V

V

P

P

F

F

R

R

D

Q

A

A

H

H

H

T

V

A

T

S

G

G

T

K

L

A

V

V

G

H

M

L

A

A

E

E

A

T

R

K

G

G

C

I

D

T

L

I

P

N

D

K

L

L

S

S

L

L

D

E

D

D

M

L

Q

K

G

S

V

I

H

S

S

P

G

Q

I

F

T

S

E

S

D

D

I

V

F

S

T

Q

V

V

L

F

G

N

V

F

E

V

N

N

S

S

V

V

N

E

S

Q

R

Y

T

T

S

A

Y

L

G

G

T

T

A

A

P

K

E

S

Q

S

V

V

R

T

A

T

Q

Q

V

I

T

E

R

Q

W

L

K

R

A

E

L

L

L

M

A

K

Q

K

active site: E71 (= E87), T144 (= T160), H145 (= H161), A266 (≠ S282), S267 (= S283), K272 (= K288), E279 (= E295)
binding argininosuccinate: R98 (= R114), N99 (= N115), V102 (= V118), T144 (= T160), H145 (= H161), Y306 (= Y322), Q311 (= Q327), K314 (= K330)

P04424 Argininosuccinate lyase; ASAL; Arginosuccinase; EC 4.3.2.1 from Homo sapiens (Human) (see 12 papers)
44% identity, 98% coverage: 6:462/464 of query aligns to 5:459/464 of P04424

query
sites
P04424

S

S

N

G

Q

K

M

L

W

W

G

G

R
|
R

F

F

A

V

A

G

G

A

P

V

D

D

A

P

I

I

M

M

E

E

A

K

I

F

N

N

A

A

S

S

I

I

G

A

F

Y

D
|
D

K

R

R

H

M

L

A

W

A

E

Q

V

D

D

I

V

Q

Q

G

G

S

S

R

K

A

A

H

Y

A

S

A

R

M

G

L

L

A

E

A
x
K

S

A

G

G

V

L

I

L

T

T

D

K

S

A

D

E

A

M

E

D

A

Q

I

I

R

L

E

H

G

G

L

L

L

D

T
x
K

I

V

L

A

S

E

E
|
E

I

W

E

A

A

Q

G

G

N

T

F

F

Q

K

F

L

S

N

T

S

A

N

L

D

E

E

D
|
D

I

I

H
|
H

M

T

N

A

V

N

E

E

A
x
R

R
|
R

L

L

K

K

E

E

I

L

I

I

G

G

E

A

P

T

A

A

G

G

R

K

L

L

H

H

T

T

G

G

R

R

S

S

R
|
R

N

N

D

D

Q

Q

V

V

A

V

T

T

D
|
D

F

L

K

R

L

L

W

W

V

M

R

R

D

Q

Q

T

L

C

D

S

A

T

A

L

E

S

T

G

G

L

L

L

L

W

A

E

L

L

I

I

A

R

A

T

L

M

L

V

D

D

Q

R

A

A

E

E

A

A

G

E

A

R

D

D

W

V

V

L

M

F

P

P

G

G

F

Y

T

T

H

H

L

L

Q

Q

T

R

A

A

Q

Q

P

P

V

I

T

R

W

W

G

S

H

H

H

W

M

I

M

L

A

S

Y

H

V

A

E
x
V

M

A

F

L

G
x
T

R
|
R

D

D

L

S

S

E

R
|
R

V

L

R

L

D

E

A

V

R

R

T

K

R

R

M

I

N

N

E

V

C

L

P

P

L

L

G
|
G

A

S

A

G

A

A

L

I

A

A

G

G

T

N

S

P

F

L

P

G

I

V

D

D

R

R

S

E

M

L

T

L

A

R

E

A

A

E

L

L

G

N

F

F

E

G

R

A

P

I

A

T

A

L

N

N

S

S

L

M

D

D

A

A

V

T

S

S

D

E

R
|
R

D
|
D

F

F

A

V

L

A

E

E

F

F

L

L

S

F

V

W

A

A

S

S

I

L

C

C

A

M

V

T

H

H

L

L

S

S

R

R

F

M

A

A

E

E

E

D

L

L

V

I

I

L

W

Y

S

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A

K

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E

F

F

R

S

F

F

V

V

T

Q

L

L

S

S

D

D

R

A

F

Y

S

S

T

T

G

G

S

S

I

L

M

M

P

P

Q
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Q

K

K

K
|
K

N

N

P

P

D

D

A

S

A

L

E

E

L

L

I

I

R
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R

A

S

K

K

V

A

G

G

R

R

I

V

F

F

G

G

A
x
R

N

C

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A

A

G

L

L

M

L

M

M

V

T

M

L

K

K

G

G

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Q

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E

D

D

K

K

E

E

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A

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V

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F

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A
x
V

A

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D

N

T

L

M

M

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L

A

A

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L

L

A

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A

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M

A

E

T

G

G

M

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V

I

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D

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M

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A

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x
M

A

G

A

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A

A

A

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G

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S

P

G

D

F

M

S

-

T

L

A

A

T

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D

D

L

L

A

A

D

Y

W

Y

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L

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V

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R

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L

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x
M

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D

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A

A

H
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H

H

E

V

A

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S

G

G

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L

A

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F

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M

A
|
A

E

E

A

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K

G

G

C

V

D

A

L

L

P

N

D

Q

L

L

S

S

L

L

D

Q

D

E

M

L

Q

Q

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I

H

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S

P

G

L

I

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T

S

E

G

D

D

I

V

F

I

T

C

V

V

L

W

G

D

V

Y

E

G

N

H

S

S

V

V

N

E

S

Q

R

Y

T

G

S

A

Y

L

G

G

T

T

A

A

P

R

E

S

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S

V

V

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A

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Q

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I

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R

R

Q

W

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x
R

A

A

L

L

R12 (= R13) to Q: in ARGINSA; 18-fold reduction in catalytic efficiency toward argininosuccinate; dbSNP:rs145138923
D31 (= D32) to N: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs754995756
K51 (≠ A52) mutation to N: 2-fold reduction in activity.
K69 (≠ T70) modified: N6-acetyllysine
E73 (= E74) to K: in ARGINSA; complete loss of argininosuccinate lyase activity; abolishes protein expression
D87 (= D88) to G: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs752100894
H89 (= H90) mutation to Q: 10-fold reduction in activity.
R94 (≠ A95) to C: in ARGINSA; severe; dbSNP:rs374304304
R95 (= R96) to C: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs28940585
R113 (= R114) to Q: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on nitric oxide production; dbSNP:rs752783461
D120 (= D121) to E: in ARGINSA; severe
V178 (≠ E179) to M: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941473
T181 (≠ G182) to S: in a breast cancer sample; somatic mutation
R182 (= R183) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs751590073
R186 (= R187) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs752397242
G200 (= G201) to V: in a breast cancer sample; somatic mutation
R236 (= R237) to W: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on NOS complex formation; dbSNP:rs761268464
D237 (= D238) to N: in ARGINSA; severe; dbSNP:rs552951774
Q286 (= Q287) to R: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941472
K288 (= K289) modified: N6-acetyllysine; mutation to R: Refractory to inhibition by TSA and NAM and by addition of extra amino acids. No effect on protein structure.
R297 (= R298) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs750431938
R306 (≠ A307) to W: in ARGINSA; severe; dbSNP:rs868834862
Q326 (= Q327) to L: in ARGINSA; severe
V335 (≠ A336) to L: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression
M360 (≠ L361) to T: in ARGINSA; loss of argininosuccinate lyase activity; may cause protein misfolding; dbSNP:rs875989948
M382 (≠ V385) to R: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression
R385 (= R388) to L: in ARGINSA; severe
H388 (= H391) to Q: in ARGINSA; severe
A398 (= A401) to D: in ARGINSA; impairs tetramer formation likely due to protein misfolding; loss of argininosuccinate lyase activity
R456 (≠ K459) to W: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs759396688

1tj7B Structure determination and refinement at 2.44 a resolution of argininosuccinate lyase from e. Coli (see paper)
47% identity, 95% coverage: 13:451/464 of query aligns to 1:439/451 of 1tj7B

query
sites
1tj7B

R

R

F

F

A

T

A

Q

G

A

P

A

D

D

A

Q

I

R

M

F

E

K

A

Q

I

F

N

N

A

D

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S

I

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G

R

F

F

D

D

K

Y

R

R

M

L

A

A

A

E

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Q

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D

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G

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A

A

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W

A

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A

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M

A

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A

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A

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G

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D

A

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E

D

E

A

Q

E

A

A

Q

I

L

R

E

E

E

G

A

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E

E

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A

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A

D

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A

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E

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A

G

G

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R

K

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L

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H

T

T

G

G

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R

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N

D

D

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A

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T

D

D

F

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K

L

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W

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A

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E

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A

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I

Q

A

S

A

A

L

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D

E

Q

T

A

A

E

Q

A

N

G

N

A

Q

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D

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A

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V

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M

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P

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G

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H
|
H

L

L

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Q

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A

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T

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F

G

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S

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M

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K

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N

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E

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G

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V

T

M

L

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K

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L

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D

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M

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D

D

K

K

E

E

Q

G

V

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F

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D

A

A

A

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D

D

N

T

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M

A

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R

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C

A

Q

A

E

A

A

G

Q

S

Q

G

G

F

Y

S

A

T

N

A

A

T

T

D

E

L

L

A

A

D

D

W

Y

L

L

V

V

R

-

V

A

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K

K

G

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V

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P

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F

R

R

D

E

A

A

H

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V

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G

G

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L

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G

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M

E

A

A

E

I

A

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Q

G

G

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D

P

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L

P

E

D

D

L

L

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L

L

D

S

D

E

M

L

Q

Q

G

K

V

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H

S

S

Q

G

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I

I

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D

E

E

D

D

I

V

F

Y

T

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I

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L

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N

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N

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Q

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V

active site: H150 (= H161), S271 (= S282), K277 (= K288), E284 (= E295)
binding phosphate ion: S271 (= S282), M274 (= M285), K277 (= K288)

1hy0A Crystal structure of wild type duck delta 1 crystallin (eye lens protein) (see paper)
43% identity, 96% coverage: 19:464/464 of query aligns to 1:444/447 of 1hy0A

query
sites
1hy0A

D

D

A

P

I

I

M

M

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Q

A

M

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A

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L

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A

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A

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A

K

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G

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A

K

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G

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E

D

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N

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N

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E

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R

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K

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G

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I

A

A

G

G

R

K

L

L

H

H

T

T

G

G

R

R

S

S

R

R

N

N

D

E

Q

Q

V

V

A

V

T

T

D

D

F

L

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K

L

L

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F

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K

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N

Q

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L

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E

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T

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L

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A

Q

L

L

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A

K

A

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L

L

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A

A

E

A

A

I

G

E

A

I

D

D

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V

V

I

M

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P

P

G

G

F

Y

T
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T

H
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H

L

L

Q

Q

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K

A

A

Q

Q

P

P

V

I

T

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W

G

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Q

H

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M

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A

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Y

H

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M

A

F

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D

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S

E

R

R

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L

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A

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K

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M

I

N

N

E

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C

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P

P

L

L

G

G

A

S

A

G

A

A

L

L

A

A

G

G

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S

P

F

L

P

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I

I

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D

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M

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A

E

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L

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F

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A

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P

I

A

S

A

L

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N

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M

D

D

A

A

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S

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R

R

D

D

F

F

A

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E

F

F

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L

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S

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V

A

A

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I

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C

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T

L

L

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S

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D

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A

F

F

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S

T

T

G

G

S
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S

I

L

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

A

S

A

L

E
|
E

L

L

I

I

R

R

A

S

K

K

V

S

G

G

R

R

I

V

F

F

G

G

A

R

N

L

T

A

A

S

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M

L

M

M

V

V

M

L

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K

G

G

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P

L

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A

T

Y

Y

S

N

K

K

D

D

M

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Q

E

E

D

D

K

K

E

E

Q

A

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V

F

I

D

D

A

V

D

D

N

T

L

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T

L

A

A

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L

L

A

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A

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M

A

E

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G

G

M

V

V

I

K

S

D

T

M

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S

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A

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N

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N

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M

A

E

A

K

A

A

A

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G

T

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P

G

E

F

M

S

-

T

L

A

A

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T

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A

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K

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F

F

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A

A

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H

H

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A

T

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G

G

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K

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M

L

A

A

E

E

A

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K

G

G

C

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D

A

L

I

P

N

D

N

L

L

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T

L

L

D

E

D

D

M

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G

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I

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G

L

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F

T

S

E

S

D

D

I

V

F

S

T

Q

V

V

L

F

G

N

V

F

E

V

N

N

S

S

V

V

N

E

S

Q

R

Y

T

T

S

A

Y

L

G

G

T

T

A

A

P

K

E

S

Q

S

V

V

R

T

A

T

Q

Q

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I

T

E

R

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W

L

K

R

A

E

L

L

L

M

A

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K

active site: E69 (= E87), T142 (= T160), H143 (= H161), S264 (= S282), S265 (= S283), K270 (= K288), E277 (= E295)
binding sulfate ion: S264 (= S282), M267 (= M285), N272 (= N290)

P02521 Delta-1 crystallin; Delta crystallin I from Gallus gallus (Chicken) (see paper)
41% identity, 99% coverage: 7:464/464 of query aligns to 6:461/466 of P02521

query
sites
P02521

N

D

Q

K

M

L

W

L

G

G

R

R

F

F

A

V

A

G

G

S

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D

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M

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A

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A

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A

A

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A

A

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A

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A

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A

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E

A

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G

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I

L

S

S

E

E

E

I

S

E

S

A

K

G

G

N

V

F

L

Q

V

F

M

S

T

T

Q

A

S

L

D

E

E

D

D

I

I

H

Q

M

T

N

A

V

I

E

E

A

R

R

R

L

L

K

K

E

E

I

L

I

I

G

G

E

D

P

I

A

A

G

G

R

K

L

L

H

Q

T

T

G

G

R

R

S

S

R

R

N

N

D

E

Q

Q

V

V

A

V

T

T

D

D

F

L

K

K

L

L

W

L

V

L

R

K

D

S

Q

S

L

I

D

S

A

V

A

I

E

S

T

T

G

H

L

L

A

Q

L

L

I

I

A

K

A

T

L

L

L

V

D

E

Q

R

A

A

E

A

A

I

G

E

A

I

D

D

W

I

V

I

M

M

P

P

G

G

F

Y

T

T

H

H

L

L

Q

Q

T

K

A

A

Q

L

P

P

V

I

T

R

W

W

G

S

H

Q

H

F

M

L

A

S

Y

H

V

A

E

V

M

A

F

L

G

T

R

R

D

D

L

S

S

E

R

R

V

L

R

G

D

E

A

V

R

K

T

K

R

R

M

I

N

T

E

V

C

L

P

P

L

L

G

G

A

S

A

G

A

A

L

L

A

A

G

G

T

N

S

P

F

L

P

E

I

I

D

D

R

R

S

E

M

L

T

L

A

R

E

S

A

E

L

L

G

D

F

M

E

T

R

S

P

I

A

T

A

L

N

N

S

S

L

I

D

D

A

A

V

I

S

S

D

E

R

R

D

D

F

F

A

V

L

V

E

E

F

L

L

I

S

S

V

V

A

A

S

T

I

L

C

L

A

M

V

I

H

H

L

L

S

S

R

K

F

L

A

A

E

E

E

D

L

L

V

I

I

I

W

F

S

S

S

T

A

T

Q

E

F

F

R

G

F

F

V

V

T

T

L

L

S

S

D

D

R

A

F

Y

S

S

T

T

G

G

S

S

I

L

M

L

P

P

Q

Q

K

K

N

N

P

P

D

D

A

S

A

L

E

E

L

L

I

I

R

R

A

S

K

K

V

A

G

G

R

R

I

V

F

F

G

G

A

R

N

L

T

A

A

A

L

I

M

L

M

M

V

V

M

L

K

K

G

G

L

I

P

P

L

S

A

T

Y

F

S

S

K

K

D

D

M

L

Q

Q

E

E

D

D

K

K

E

E

Q

A

V

V

F

L

D

D

A

V

D

D

N

T

L

L

M

T

L

A

A

V

L

L

A

Q

A

V

M

A

E

T

G

G

M

V

V

I

K

S

D

T

M

L

S

Q

A

V

N

N

R

K

E

E

S

N

L

M

A

E

A

K

A

A

A

L

G

T

S

P

G

E

F

L

S

-

T

L

A

S

T

T

D

D

L

L

A

A

D

L

W

Y

L

L

V

V

R

R

V

K

L

-

K

G

V

M

P

P

F

I

R

R

D

Q

A

A

H

Q

H

T

V

A

T

S

G

G

T

K

L

A

V

V

G

H

M

L

A

A

E

E

A

T

R

K

G

G

C

I

D

T

L

I

P

N

D

N

L

L

S

T

L

L

D

E

D

D

M

L

Q

K

G

S

V

I

H

S

S

P

G

L

I

F

T

A

E

S

D

D

I

V

F

S

T

Q

V

V

L

F

G

S

V

V

E

V

N

N

S

S

V

V

N

E

S

Q

R

Y

T

T

S

A

Y

V

G

G

T

T

A

A

P

K

E

S

Q

S

V

V

R

T

A

A

Q

Q

V

I

T

E

R

Q

W

L

K

R

A

E

L

L

A

K

Q

K

Sites not aligning to the query:

2 modified: Blocked amino end (Ala)

6ienB Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
43% identity, 95% coverage: 18:456/464 of query aligns to 1:439/454 of 6ienB

query
sites
6ienB

P

P

D

S

A

D

I

A

M

L

E

A

A

A

I

L

N

S

A

K

S

S

I

T

G

H

F

F

D

D

K

W

R

V

M

L

A

A

A

P

Q

Y

D

D

I

L

Q

T

G

A

S

S

R

R

A

A

H

H

A

T

A

M

M

V

L

L

A

F

A

R

S

A

G

G

V

L

I

L

T

T

D

E

S

E

D

Q

A

R

E

D

A

G

I

L

R

L

E

A

G

G

L

L

L

D

T

S

I

L

L

A

S

Q

E

D

I

V

E

A

A

D

G

G

N

S

F

F

Q

G

F

P

S

L

T

V

A

T

L

D

E

E

D

D

I

V

H

H

M

A

N

A

V

L

E

E

A

R

R

G

L

L

K

I

E

D

I

R

I

V

G

G

-

P

E

D

P

L

A

G

G

G

R

R

L

L

H

R

T

A

G

G

R

R

S
|
S

R
|
R

N
|
N

D

D

Q

Q

V

V

A

A

T

A

D

L

F

F

K

R

L

M

W

W

V

L

R

R

D

D

Q

A

L

V

D

R

A

R

A

V

E

A

T

T

G

G

L

V

L

L

A

D

L

V

I

V

A

G

A

A

L

L

L

A

D

E

Q

Q

A

A

E

A

A

A

G

H

A

P

D

S

W

A

V

I

M

M

P

P

G

G

F

K

T
|
T

H
|
H

L

L

Q

Q

T

S

A

A

Q

Q

P

P

V

I

T

L

W

L

G

A

H

H

M

L

A

A

Y

H

V

A

E

H

M

P

F

L

G

L

R

R

D

D

L

L

S

D

R

R

V

I

R

V

D

D

A

F

R

D

T

K

R

R

M

A

N

A

E

V

C

S

P

P

L

Y

G

G

A

S

A

G

A

A

L

L

A

A

G

G

T

S

S

S

F

L

P

G

I

L

D

D

R

P

S

D

M

A

T

I

A

A

E

A

A

D

L

L

G

G

F

F

E

S

R

A

P

A

A

A

A

D

N

N

S

S

L

V

D

D

A

A

V

T

S

A

D

A

R

R

D

D

F

F

A

A

L

A

E

E

F

A

L

A

S

F

V

V

A

F

S

A

I

M

C

I

A

A

V

V

H

D

L

L

S

S

R

R

F

L

A

A

E

E

E

D

L

I

V

I

I

V

W

W

S

S

A

T

Q

E

F

F

R

G

F

Y

V

V

T

T

L

L

S

H

D

D

R

S

F

W

S

S

T

T

G

G

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N

N

P

P

D

D

A

I

A

A

E

E

L

L

I

A

R

R

A

G

K

K

V

S

G

G

R

R

I

L

F

I

G

G

A

N

N

L

T

A

A

G

L

L

M

L

M

A

V

T

M

L

K

K

G

A

L

Q

P

P

L

L

A

A

Y
|
Y

S

N

K

R

D

D

M

L

Q
|
Q

E

E

D

D

K
|
K

E

E

Q

P

V

V

F

F

D

D

A

S

A

V

D

A

N

Q

L

L

M

E

L

L

A

L

L

L

A

P

A

A

M

M

E

A

G

G

M

L

V

V

K

A

D

S

M

L

S

T

A

F

N

N

R

V

E

Q

S

R

L

M

A

A

A

E

A

L

A

A

G

P

S

A

G

G

F

Y

S

T

T

L

A

A

T

T

D

D

L

L

A

A

D

E

W

W

L

L

V

V

R

R

V

Q

L

-

K

G

V

V

P

P

F

F

R

R

D

S

A

A

H

H

H

E

V

A

T

A

G

G

T

A

L

A

V

V

G

R

M

A

A

A

E

E

A

Q

R

R

G

G

C

V

D

G

L

L

P

Q

D

E

L

L

S

T

L

D

D

D

D

E

M

L

Q

A

G

A

V

I

H

S

S

P

G

E

I

L

T

T

E

P

D

Q

I

V

F

R

T

E

V

V

L

L

G

T

V

I

E

E

N

G

S

S

V

V

N

S

S

A

R

R

T

D

S

C

Y

R

G

G

T

T

A

A

P

P

E

G

Q

R

V

V

R

A

A

E

Q

Q

V

L

T

N

binding argininosuccinate: S97 (= S113), R98 (= R114), N99 (= N115), T144 (= T160), H145 (= H161), S266 (= S282), S267 (= S283), M269 (= M285), K272 (= K288), Y306 (= Y322), Q311 (= Q327), K314 (= K330)

6ienA Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
43% identity, 95% coverage: 18:456/464 of query aligns to 1:437/452 of 6ienA

query
sites
6ienA

P

P

D

S

A

D

I

A

M

L

E

A

A

A

I

L

N

S

A

K

S

S

I

T

G

H

F

F

D

D

K

W

R

V

M

L

A

A

A

P

Q

Y

D

D

I

L

Q

T

G

A

S

S

R

R

A

A

H

H

A

T

A

M

M

V

L

L

A

F

A

R

S

A

G

G

V

L

I

L

T

T

D

E

S

E

D

Q

A

R

E

D

A

G

I

L

R

L

E

A

G

G

L

L

L

D

T

S

I

L

L

A

S

Q

E

D

I

V

E

A

A

D

G

G

N

S

F

F

Q

G

F

P

S

L

T

V

A

T

L

D

E

E

D

D

I

V

H

H

M

A

N

A

V

L

E

E

A

R

R

G

L

L

K

I

E

D

I

R

I

V

G

G

-

P

E

D

P

L

A

G

G

G

R

R

L

L

H

R

T

A

G

G

R

R

S

S

R
|
R

N
|
N

D

D

Q

Q

V
|
V

A

A

T

A

D

L

F

F

K

R

L

M

W

W

V

L

R

R

D

D

Q

A

L

V

D

R

A

R

A

V

E

A

T

T

G

G

L

V

L

L

A

D

L

V

I

V

A

G

A

A

L

L

L

A

D

E

Q

Q

A

A

E

A

A

A

G

H

A

P

D

S

W

A

V

I

M

M

P

P

G

G

F

K

T
|
T

H
|
H

L

L

Q

Q

T

S

A

A

Q

Q

P

P

V

I

T

L

W

L

G

A

H

H

M

L

A

A

Y

H

V

A

E

H

M

P

F

L

G

L

R

R

D

D

L

L

S

D

R

R

V

I

R

V

D

D

A

F

R

D

T

K

R

R

M

A

N

A

E

V

C

S

P

P

L

Y

G

G

A

S

A

G

A

A

L

L

A

A

G

G

T

S

S

S

F

L

P

G

I

L

D

D

R

P

S

D

M

A

T

I

A

A

E

A

A

D

L

L

G

G

F

F

E

S

R

A

P

A

A

A

A

D

N

N

S

S

L

V

D

D

A

A

V

T

S

A

D

A

R

R

D

D

F

F

A

A

L

A

E

E

F

A

L

A

S

F

V

V

A

F

S

A

I

M

C

I

A

A

V

V

H

D

L

L

S

S

R

R

F

L

A

A

E

E

E

D

L

I

V

I

I

V

W

W

S

S

A

T

Q

E

F

F

R

G

F

Y

V

V

T

T

L

L

S

H

D

D

R

S

F

W

S

S

T

T

G

G

S
|
S

I

-

M

-

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

A

I

A

A

E

E

L

L

I

A

R

R

A

G

K

K

V

S

G

G

R

R

I

L

F

I

G

G

A

N

N

L

T

A

A

G

L

L

M

L

M

A

V

T

M

L

K

K

G

A

L

Q

P

P

L

L

A

A

Y
|
Y

S

N

K

R

D

D

M

L

Q
|
Q

E

E

D

D

K
|
K

E

E

Q

P

V

V

F

F

D

D

A

S

A

V

D

A

N

Q

L

L

M

E

L

L

A

L

L

L

A

P

A

A

M

M

E

A

G

G

M

L

V

V

K

A

D

S

M

L

S

T

A

F

N

N

R

V

E

Q

S

R

L

M

A

A

A

E

A

L

A

A

G

P

S

A

G

G

F

Y

S

T

T

L

A

A

T

T

D

D

L

L

A

A

D

E

W

W

L

L

V

V

R

R

V

Q

L

-

K

G

V

V

P

P

F

F

R

R

D

S

A

A

H

H

H

E

V

A

T

A

G

G

T

A

L

A

V

V

G

R

M

A

A

A

E

E

A

Q

R

R

G

G

C

V

D

G

L

L

P

Q

D

E

L

L

S

T

L

D

D

D

D

E

M

L

Q

A

G

A

V

I

H

S

S

P

G

E

I

L

T

T

E

P

D

Q

I

V

F

R

T

E

V

V

L

L

G

T

V

I

E

E

N

G

S

S

V

V

N

S

S

A

R

R

T

D

S

C

Y

R

G

G

T

T

A

A

P

P

E

G

Q

R

V

V

R

A

A

E

Q

Q

V

L

T

N

binding argininosuccinate: R98 (= R114), N99 (= N115), V102 (= V118), T144 (= T160), H145 (= H161), Y304 (= Y322), Q309 (= Q327), K312 (= K330)
binding fumaric acid: S266 (= S282), S267 (= S283), K270 (= K288), N272 (= N290)

6ienC Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
44% identity, 84% coverage: 18:406/464 of query aligns to 1:389/418 of 6ienC

query
sites
6ienC

P

P

D

S

A

D

I

A

M

L

E

A

A

A

I

L

N

S

A

K

S

S

I

T

G

H

F

F

D

D

K

W

R

V

M

L

A

A

A

P

Q

Y

D

D

I

L

Q

T

G

A

S

S

R

R

A

A

H

H

A

T

A

M

M

V

L

L

A

F

A

R

S

A

G

G

V

L

I

L

T

T

D

E

S

E

D

Q

A

R

E

D

A

G

I

L

R

L

E

A

G

G

L

L

L

D

T

S

I

L

L

A

S

Q

E

D

I

V

E

A

A

D

G

G

N

S

F

F

Q

G

F

P

S

L

T

V

A

T

L

D

E

E

D

D

I

V

H

H

M

A

N

A

V

L

E

E

A

R

R

G

L

L

K

I

E

D

I

R

I

V

G

G

-

P

E

D

P

L

A

G

G

G

R

R

L

L

H

R

T

A

G

G

R

R

S
|
S

R
|
R

N
|
N

D

D

Q

Q

V
|
V

A

A

T

A

D

L

F

F

K

R

L

M

W

W

V

L

R

R

D

D

Q

A

L

V

D

R

A

R

A

V

E

A

T

T

G

G

L

V

L

L

A

D

L

V

I

V

A

G

A

A

L

L

L

A

D

E

Q

Q

A

A

E

A

A

A

G

H

A

P

D

S

W

A

V

I

M

M

P

P

G

G

F

K

T
|
T

H
|
H

L

L

Q

Q

T

S

A

A

Q

Q

P

P

V

I

T

L

W

L

G

A

H

H

M

L

A

A

Y

H

V

A

E

H

M

P

F

L

G

L

R

R

D

D

L

L

S

D

R

R

V

I

R

V

D

D

A

F

R

D

T

K

R

R

M

A

N

A

E

V

C

S

P

P

L

Y

G

G

A

S

A

G

A

A

L

L

A

A

G

G

T

S

S

S

F

L

P

G

I

L

D

D

R

P

S

D

M

A

T

I

A

A

E

A

A

D

L

L

G

G

F

F

E

S

R

A

P

A

A

A

A

D

N

N

S

S

L

V

D

D

A

A

V

T

S

A

D

A

R

R

D

D

F

F

A

A

L

A

E

E

F

A

L

A

S

F

V

V

A

F

S

A

I

M

C

I

A

A

V

V

H

D

L

L

S

S

R

R

F

L

A

A

E

E

E

D

L

I

V

I

I

V

W

W

S

S

A

T

Q

E

F

F

R

G

F

Y

V

V

T

T

L

L

S

H

D

D

R

S

F

W

S

S

T

T

G

G

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N

N

P

P

D

D

A

I

A

A

E

E

L

L

I

A

R

R

A

G

K

K

V

S

G

G

R

R

I

L

F

I

G

G

A

N

N

L

T

A

A

G

L

L

M

L

M

A

V

T

M

L

K

K

G

A

L

Q

P

P

L

L

A

A

Y
|
Y

S

N

K

R

D

D

M

L

Q
|
Q

E

E

D

D

K
|
K

E

E

Q

P

V

V

F

F

D

D

A

S

A

V

D

A

N

Q

L

L

M

E

L

L

A

L

L

L

A

P

A

A

M

M

E

A

G

G

M

L

V

V

K

A

D

S

M

L

S

T

A

F

N

N

R

V

E

Q

S

R

L

M

A

A

A

E

A

L

A

A

G

P

S

A

G

G

F

Y

S

T

T

L

A

A

T

T

D

D

L

L

A

A

D

E

W

W

L

L

V

V

R

R

V

Q

L

-

K

G

V

V

P

P

F

F

R

R

D

S

A

A

H

H

H

R

V

E

T

V

G

L

T

T

L

I

V

E

G

G

M

S

A

V

E

S

A

A

R

R

G

D

C

C

binding arginine: R98 (= R114), N99 (= N115), V102 (= V118), Y306 (= Y322), Q311 (= Q327), K314 (= K330)
binding argininosuccinate: T144 (= T160), H145 (= H161), S266 (= S282), S267 (= S283), M269 (= M285), K272 (= K288)
binding fumaric acid: S97 (= S113), R98 (= R114), N99 (= N115)

6g3iA Crystal structure of edds lyase in complex with n-(2-aminoethyl) aspartic acid (aeaa) (see paper)
34% identity, 90% coverage: 39:455/464 of query aligns to 39:454/496 of 6g3iA

query
sites
6g3iA

D

D

I

S

Q

Q

G

I

S

H

R

R

A

A

H

H

A

V

M

M

L

L

A

T

A

E

S

Q

G

G

V

I

I

L

T

T

D

V

S

E

D

E

A

S

E

A

A

T

I

I

R

L

E

S

G

G

L

L

L

A

T

Q

I

V

L

-

S

D

E

E

I

L

E

A

A

A

G

T

N

D

F

G

Q

S

F

L

S

R

T

T

A

-

L

-

E

-

D

-

I

-

H

Y

M

L

N

P

V

Y

E

E

A

A

R

A

L

L

K

K

E

R

I

T

I

I

G

G

E

S

P

V

A

A

G

G

R

K

L

M

H

H

T

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

T

A

D

G

F

K

K

R

L

M

W

F

V

L

R

R

D

D

Q

Q

L

L

D

L

A

R

A

T

E

I

T

E

G

A

L

V

L

I

A

G

L

Y

I

R

A

E

A

A

L

V

D

H

Q

K

A

A

E

A

A

D

G

H

A

L

D

D

W

T

V

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

T

E

A

A

Q

Q

P

P

V

I

T

T

W

L

G

G

H

H

H

Y

M

L

M

M

A

A

Y

I

V

S

E

E

M

N

F

L

G

A

R

K

D

N

L

L

S

D

R

R

V

Y

R

R

D

E

A

L

R

Y

T

A

R

R

M

I

N

N

E

L

C

C

P

P

L

L

G

G

A

A

L

T

A

A

G

G

T

T

S

G

F

W

P

P

I

L

D

N

R

R

S

D

M

R

T

T

A

S

E

A

A

L

L

L

G

G

F

F

E

D

R

G

P

L

A

V

N

N

S

S

L

I

D

E

A

G

V

V

S

A

D

G

R

W

D

D

F

H

A

V

L

A

E

E

F

H

L

A

S

F

V

V

A

N

S

A

I

V

C

F

A

L

V

S

H

G

L

L

S

S

R

R

F

L

A

A

E

S

E

E

L

I

V

Q

I

L

W

W

S

S

S

T

A

D

Q

E

F

Y

R

Q

F

V

V

A

T

E

L

L

S

D

D

A

R

S

F

F

S

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D
|
D

A

S

A

L

E

E

L
x
R

I

S

R

R

A

K

K

A

V

A

G

F

R

A

I

A

F

M

G

G

A

P

N

L

T

V

A

G

L

I

M

L

M

T

V

S

M

L

K

N

G

A

L

I

P

E

L

Y

A

Q

Y

Y

S

S

K

A

D

A

M

R

Q

V

E

E

D

L

K

E

E

P

Q

R

V

S

F

I

D

D

A

A

A

-

D

-

N

-

L

L

M

I

L

A

A

A

L

T

A

H

A

A

M

M

E

T

G

G

M

V

V

V

K

R

D

T

M

L

S

H

A

P

N

N

R

K

E

E

S

R

L

M

A

R

A

Q

A

Y

A

A

G

A

S

E

G

N

F

Y

S

S

T

T

A

M

T

T

D

D

L

L

A

T

D

D

W

M

L

L

V

V

R

R

V

R

L

V

K

G

V

I

P

D

F

Y

R

R

D

E

A

A

H

H

H

E

V

I

T

V

G

A

T

H

L

V

V

V

G

I

M

T

A

A

E

I

A

E

R

K

G

G

C

I

D

K

L

A

P

N

D

K

L

I

S

G

L

L

D

D

D

L

M

V

Q

Q

-

E

-

A

-

V

-

A

G

Q

V

T

H

G

S

A

G

G

I

I

T

N

-

V

-

S

-

A

E

D

D

D

I

I

F

K

T

D

V

A

L

L

G

D

V

P

E

W

N

Q

S

N

V

V

N

L

S

R

R

R

T

E

S

G

Y

K

G

G

G

M

T

P

A

A

P

P

E

M

Q

S

V

V

R

K

A

A

Q

S

V

I

binding (2~{S})-2-(2-azanylethylamino)butanedioic acid: S276 (= S282), S277 (= S283), M279 (= M285), K282 (= K288), N284 (= N290), D286 (= D292)
binding fumaric acid: R290 (≠ L296)

Sites not aligning to the query:

binding fumaric acid: 22

6g3hA Crystal structure of edds lyase in complex with ss-edds (see paper)
34% identity, 90% coverage: 39:455/464 of query aligns to 39:454/497 of 6g3hA

query
sites
6g3hA

D

D

I

S

Q

Q

G

I

S

H

R

R

A

A

H

H

A

V

M

M

L

L

A

T

A

E

S

Q

G

G

V

I

I

L

T

T

D

V

S

E

D

E

A

S

E

A

A

T

I

I

R

L

E

S

G

G

L

L

L

A

T

Q

I

V

L

-

S

D

E

E

I

L

E

A

A

A

G

T

N

D

F

G

Q

S

F

L

S

R

T

T

A

-

L

-

E

-

D

-

I

-

H

Y

M

L

N

P

V

Y

E

E

A

A

R

A

L

L

K

K

E

R

I

T

I

I

G

G

E

S

P

V

A

A

G

G

R

K

L

M

H

H

T

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

T

A

D

G

F

K

K

R

L

M

W

F

V

L

R

R

D

D

Q

Q

L

L

D

L

A

R

A

T

E

I

T

E

G

A

L

V

L

I

A

G

L

Y

I

R

A

E

A

A

L

V

D

H

Q

K

A

A

E

A

A

D

G

H

A

L

D

D

W

T

V

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

T

E

A

A

Q

Q

P

P

V

I

T

T

W

L

G

G

H

H

H

Y

M

L

M

M

A

A

Y

I

V

S

E

E

M

N

F

L

G

A

R

K

D

N

L

L

S

D

R

R

V

Y

R

R

D

E

A

L

R

Y

T

A

R

R

M

I

N

N

E

L

C

C

P

P

L

L

G

G

A

A

L

T

A

A

G

G

T

T

S

G

F

W

P

P

I

L

D

N

R

R

S

D

M

R

T

T

A

S

E

A

A

L

L

L

G

G

F

F

E

D

R

G

P

L

A

V

N

N

S

S

L

I

D

E

A

G

V

V

S

A

D

G

R

W

D

D

F

H

A

V

L

A

E

E

F

H

L

A

S

F

V

V

A

N

S

A

I

V

C

F

A

L

V

S

H

G

L

L

S

S

R

R

F

L

A

A

E

S

E

E

L

I

V

Q

I

L

W

W

S

S

S

T

A

D

Q

E

F

Y

R

Q

F

V

V

A

T

E

L

L

S

D

D

A

R

S

F

F

S

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D
|
D

A

S

A

L

E

E

L
x
R

I

S

R

R

A

K

K

A

V

A

G

F

R

A

I

A

F

M

G

G

A

P

N

L

T

V

A

G

L

I

M

L

M

T

V

S

M

L

K

N

G

A

L

I

P

E

L

Y

A

Q

Y

Y

S

S

K

A

D

A

M

R

Q

V

E

E

D

L

K

E

E

P

Q

R

V

S

F

I

D

D

A

A

A

-

D

-

N

-

L

L

M

I

L

A

A

A

L

T

A

H

A

A

M

M

E

T

G

G

M

V

V

V

K

R

D

T

M

L

S

H

A

P

N

N

R

K

E

E

S

R

L

M

A

R

A

Q

A

Y

A

A

G

A

S

E

G

N

F

Y

S

S

T

T

A

M

T

T

D

D

L

L

A

T

D

D

W

M

L

L

V

V

R

R

V

R

L

V

K

G

V

I

P

D

F

Y

R

R

D

E

A

A

H

H

H

E

V

I

T

V

G

A

T

H

L

V

V

V

G

I

M

T

A

A

E

I

A

E

R

K

G

G

C

I

D

K

L

A

P

N

D

K

L

I

S

G

L

L

D

D

D

L

M

V

Q

Q

-

E

-

A

-

V

-

A

G

Q

V

T

H

G

S

A

G

G

I

I

T

N

-

V

-

S

-

A

E

D

D

D

I

I

F

K

T

D

V

A

L

L

G

D

V

P

E

W

N

Q

S

N

V

V

N

L

S

R

R

R

T

E

S

G

Y

K

G

G

G

M

T

P

A

A

P

P

E

M

Q

S

V

V

R

K

A

A

Q

S

V

I

binding (2~{S})-2-[2-[[(2~{S})-1,4-bis(oxidanyl)-1,4-bis(oxidanylidene)butan-2-yl]amino]ethylamino]butanedioic acid: S276 (= S282), S277 (= S283), M279 (= M285), K282 (= K288), N284 (= N290), D286 (= D292), R290 (≠ L296)

Sites not aligning to the query:

binding (2~{S})-2-[2-[[(2~{S})-1,4-bis(oxidanyl)-1,4-bis(oxidanylidene)butan-2-yl]amino]ethylamino]butanedioic acid: 22

6g3gA Crystal structure of edds lyase in complex with succinate (see paper)
34% identity, 90% coverage: 39:455/464 of query aligns to 39:454/497 of 6g3gA

query
sites
6g3gA

D

D

I

S

Q

Q

G

I

S

H

R

R

A

A

H

H

A

V

M

M

L

L

A

T

A

E

S

Q

G

G

V

I

I

L

T

T

D

V

S

E

D

E

A

S

E

A

A

T

I

I

R

L

E

S

G

G

L

L

L

A

T

Q

I

V

L

-

S

D

E

E

I

L

E

A

A

A

G

T

N

D

F

G

Q

S

F

L

S

R

T

T

A

-

L

-

E

-

D

-

I

-

H

Y

M

L

N

P

V

Y

E

E

A

A

R

A

L

L

K

K

E

R

I

T

I

I

G

G

E

S

P

V

A

A

G

G

R

K

L

M

H

H

T

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

T

A

D

G

F

K

K

R

L

M

W

F

V

L

R

R

D

D

Q

Q

L

L

D

L

A

R

A

T

E

I

T

E

G

A

L

V

L

I

A

G

L

Y

I

R

A

E

A

A

L

V

D

H

Q

K

A

A

E

A

A

D

G

H

A

L

D

D

W

T

V

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

T

E

A

A

Q

Q

P

P

V

I

T

T

W

L

G

G

H

H

H

Y

M

L

M

M

A

A

Y

I

V

S

E

E

M

N

F

L

G

A

R

K

D

N

L

L

S

D

R

R

V

Y

R

R

D

E

A

L

R

Y

T

A

R

R

M

I

N

N

E

L

C

C

P

P

L

L

G

G

A

A

L

T

A

A

G

G

T

T

S

G

F

W

P

P

I

L

D

N

R

R

S

D

M

R

T

T

A

S

E

A

A

L

L

L

G

G

F

F

E

D

R

G

P

L

A

V

N

N

S

S

L

I

D

E

A

G

V

V

S

A

D

G

R

W

D

D

F

H

A

V

L

A

E

E

F

H

L

A

S

F

V

V

A

N

S

A

I

V

C

F

A

L

V

S

H

G

L

L

S

S

R

R

F

L

A

A

E

S

E

E

L

I

V

Q

I

L

W

W

S

S

S

T

A

D

Q

E

F

Y

R

Q

F

V

V

A

T

E

L

L

S

D

D

A

R

S

F

F

S

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

A

S

A

L

E

E

L

R

I

S

R

R

A

K

K

A

V

A

G

F

R

A

I

A

F

M

G

G

A

P

N

L

T

V

A

G

L

I

M

L

M

T

V

S

M

L

K

N

G

A

L

I

P

E

L

Y

A

Q

Y

Y

S

S

K

A

D

A

M

R

Q

V

E

E

D

L

K

E

E

P

Q

R

V

S

F

I

D

D

A

A

A

-

D

-

N

-

L

L

M

I

L

A

A

A

L

T

A

H

A

A

M

M

E

T

G

G

M

V

V

V

K

R

D

T

M

L

S

H

A

P

N

N

R

K

E

E

S

R

L

M

A

R

A

Q

A

Y

A

A

G

A

S

E

G

N

F

Y

S

S

T

T

A

M

T

T

D

D

L

L

A

T

D

D

W

M

L

L

V

V

R

R

V

R

L

V

K

G

V

I

P

D

F

Y

R

R

D

E

A

A

H

H

H

E

V

I

T

V

G

A

T

H

L

V

V

V

G

I

M

T

A

A

E

I

A

E

R

K

G

G

C

I

D

K

L

A

P

N

D

K

L

I

S

G

L

L

D

D

D

L

M

V

Q

Q

-

E

-

A

-

V

-

A

G

Q

V

T

H

G

S

A

G

G

I

I

T

N

-

V

-

S

-

A

E

D

D

D

I

I

F

K

T

D

V

A

L

L

G

D

V

P

E

W

N

Q

S

N

V

V

N

L

S

R

R

R

T

E

S

G

Y

K

G

G

G

M

T

P

A

A

P

P

E

M

Q

S

V

V

R

K

A

A

Q

S

V

I

binding succinic acid: S276 (= S282), S277 (= S283), M279 (= M285), K282 (= K288), N284 (= N290)

6g3fA Crystal structure of edds lyase in complex with fumarate (see paper)
34% identity, 90% coverage: 39:455/464 of query aligns to 39:454/497 of 6g3fA

query
sites
6g3fA

D

D

I

S

Q

Q

G

I

S

H

R

R

A

A

H

H

A

V

M

M

L

L

A

T

A

E

S

Q

G

G

V

I

I

L

T

T

D

V

S

E

D

E

A

S

E

A

A

T

I

I

R

L

E

S

G

G

L

L

L

A

T

Q

I

V

L

-

S

D

E

E

I

L

E

A

A

A

G

T

N

D

F

G

Q

S

F

L

S

R

T

T

A

-

L

-

E

-

D

-

I

-

H

Y

M

L

N

P

V

Y

E

E

A

A

R

A

L

L

K

K

E

R

I

T

I

I

G

G

E

S

P

V

A

A

G

G

R

K

L

M

H

H

T

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

T

A

D

G

F

K

K

R

L

M

W

F

V

L

R

R

D

D

Q

Q

L

L

D

L

A

R

A

T

E

I

T

E

G

A

L

V

L

I

A

G

L

Y

I

R

A

E

A

A

L

V

D

H

Q

K

A

A

E

A

A

D

G

H

A

L

D

D

W

T

V

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

T

E

A

A

Q

Q

P

P

V

I

T

T

W

L

G

G

H

H

H

Y

M

L

M

M

A

A

Y

I

V

S

E

E

M

N

F

L

G

A

R

K

D

N

L

L

S

D

R

R

V

Y

R

R

D

E

A

L

R

Y

T

A

R

R

M

I

N

N

E

L

C

C

P

P

L

L

G

G

A

A

L

T

A

A

G

G

T

T

S

G

F

W

P

P

I

L

D

N

R

R

S

D

M

R

T

T

A

S

E

A

A

L

L

L

G

G

F

F

E

D

R

G

P

L

A

V

N

N

S

S

L

I

D

E

A

G

V

V

S

A

D

G

R

W

D

D

F

H

A

V

L

A

E

E

F

H

L

A

S

F

V

V

A

N

S

A

I

V

C

F

A

L

V

S

H

G

L

L

S

S

R

R

F

L

A

A

E

S

E

E

L

I

V

Q

I

L

W

W

S

S

S

T

A

D

Q

E

F

Y

R

Q

F

V

V

A

T

E

L

L

S

D

D

A

R

S

F

F

S

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

A

S

A

L

E

E

L

R

I

S

R

R

A

K

K

A

V

A

G

F

R

A

I

A

F

M

G

G

A

P

N

L

T

V

A

G

L

I

M

L

M

T

V

S

M

L

K

N

G

A

L

I

P

E

L

Y

A

Q

Y

Y

S

S

K

A

D

A

M

R

Q

V

E

E

D

L

K

E

E

P

Q

R

V

S

F

I

D

D

A

A

A

-

D

-

N

-

L

L

M

I

L

A

A

A

L

T

A

H

A

A

M

M

E

T

G

G

M

V

V

V

K

R

D

T

M

L

S

H

A

P

N

N

R

K

E

E

S

R

L

M

A

R

A

Q

A

Y

A

A

G

A

S

E

G

N

F

Y

S

S

T

T

A

M

T

T

D

D

L

L

A

T

D

D

W

M

L

L

V

V

R

R

V

R

L

V

K

G

V

I

P

D

F

Y

R

R

D

E

A

A

H

H

H

E

V

I

T

V

G

A

T

H

L

V

V

V

G

I

M

T

A

A

E

I

A

E

R

K

G

G

C

I

D

K

L

A

P

N

D

K

L

I

S

G

L

L

D

D

D

L

M

V

Q

Q

-

E

-

A

-

V

-

A

G

Q

V

T

H

G

S

A

G

G

I

I

T

N

-

V

-

S

-

A

E

D

D

D

I

I

F

K

T

D

V

A

L

L

G

D

V

P

E

W

N

Q

S

N

V

V

N

L

S

R

R

R

T

E

S

G

Y

K

G

G

G

M

T

P

A

A

P

P

E

M

Q

S

V

V

R

K

A

A

Q

S

V

I

binding fumaric acid: S276 (= S282), S277 (= S283), M279 (= M285), K282 (= K288), N284 (= N290)

3r6vG Crystal structure of aspartase from bacillus sp. Ym55-1 with bound l- aspartate (see paper)
27% identity, 58% coverage: 40:306/464 of query aligns to 33:339/463 of 3r6vG

query
sites
3r6vG

I

I

Q

T

G

G

S

Y

R

R

A

I

H

H

A

P

A

E

M

L

L

I

A

K

A

S

S

L

G

G

V

I

I

V

T

K

D

K

S

S

D

A

A

A

E

L

A

A

I

N

R

M

E

E

-

V

G

G

L

L

T

-

I

-

L

-

S

-

E

D

I

K

E

E

A

V

G

G

N

Q

F

Y

Q

I

F

V

S

K

T

A

A

A

L

D

E

E

-

V

-

I

-

E

-

G

-

K

-

W

-

N

-

D

-

Q

-

F

-

I

-

V

-

D

-

P

-

I

-

Q

-

G

-

A

-

G

-

T

D

S

I

I

H

N

M

M

N

N

-

A

-

N

-

E

-

V

V

I

E

A

A

N

R

R

L

A

K

L

E

E

I

L

I

M

G

G

E

E

-

E

-

K

-

G

-

N

-

Y

-

S

-

K

-

I

-

S

P

P

A

N

G

S

R

H

L

V

H

N

T

M

G

S

R

Q

S

S

R

T

N

N

D

D

Q

A

V

F

A

P

T

T

D

A

F

T

K

H

L

I

W

A

V

V

R

L

D

S

Q

L

L

L

D

N

A

Q

A

L

E

I

T

E

G

T

L

T

L

K

A

Y

L

M

I

Q

A

Q

A

E

L

F

L

M

D

K

Q

K

A

A

E

D

A

E

G

F

A

A

D

G

W

V

V

I

M

K

P

M

G

G

F

R

T

T

H
|
H

L

L

Q

Q

T

D

A

A

Q

V

P

P

V

I

T

L

W

L

G

G

H

Q

H

E

M

F

M

E

A

A

Y

Y

V

A

E

R

M

V

F

I

G

A

R

R

D

D

L

I

S

E

R

R

V

I

R

A

D

N

A

T

R

R

T

N

R

N

M

L

N

Y

E

D

C

I

P

N

L

M

G

G

A

A

A

T

A

A

L

V

A

-

G

G

T

T

S

G

F

L

P

N

I

A

D

D

R

P

-

E

-

Y

-

I

S

S

M

I

T

V

A

T

E

E

A

H

L

L

G

A

-

K

F

F

E

S

-

G

-

H

-

P

-

L

R

R

P

S

A

A

A

Q

N

H

S

L

L

V

D

D

A

A

V

T

S

Q

D

N

R

T

D

D

F

C

A

Y

L

T

E

E

F

V

L

S

S

S

V

A

A

L

S

K

I

V

C

C

A

M

V

I

H

N

L

M

S

S

R

K

F

I

A

A

E

N

E

D

L

L

V

R

I

L

W

M

S

A

S

S

-

G

-

P

-

R

A

A

Q

G

F

L

R

S

F

E

V

I

T

V

L

L

S

P

D

A

R

R

F

-

S

Q

T

P

G
|
G

S
|
S

I

I

M

M

P

P

Q

G

K
|
K

K

V

N

N

P

P

D

V

A

M

A

P

E
|
E

L

V

I

M

R

N

A

Q

K

V

V

A

G

F

R

Q

I

V

F

F

G

G

active site: H185 (= H161), S315 (= S282), K321 (= K288), E328 (= E295)
binding aspartic acid: G314 (= G281), S315 (= S282), S316 (= S283)

Q9LCC6 Aspartate ammonia-lyase; Aspartase; EC 4.3.1.1 from Bacillus sp. (see 3 papers)
27% identity, 58% coverage: 40:306/464 of query aligns to 36:342/468 of Q9LCC6

query
sites
Q9LCC6

I

I

Q

T

G

G

S

Y

R

R

A

I

H

H

A

P

A

E

M

L

L

I

A

K

A

S

S

L

G

G

V

I

I

V

T

K

D

K

S

S

D

A

A

A

E

L

A

A

I

N

R

M

E

E

-

V

G

G

L

L

T

-

I

-

L

-

S

-

E

D

I

K

E

E

A

V

G

G

N

Q

F

Y

Q

I

F

V

S

K

T

A

A

A

L

D

E

E

-

V

-

I

-

E

-

G

-

K

-

W

-

N

-

D

-

Q

-

F

-

I

-

V

-

D

-

P

-

I

-

Q

-

G

-

A

-

G

-
x
T

D

S

I

I

H

N

M

M

N

N

-

A

-

N

-

E

-

V

V

I

E

A

A

N

R

R

L

A

K

L

E

E

I

L

I

M

G

G

E

E

-

E

-

K

-

G

-

N

-

Y

-

S

-

K

-

I

-

S

P

P

A

N

G

S

R
x
H

L

V

H

N

T

M

G

S

R

Q

S
|
S

R
x
T

N
|
N

D

D

Q

A

V

F

A

P

T

T

D

A

F

T

K

H

L

I

W

A

V

V

R

L

D

S

Q

L

L

L

D

N

A

Q

A

L

E

I

T

E

G

T

L

T

L

K

A

Y

L

M

I

Q

A

Q

A

E

L

F

L

M

D

K

Q

K

A

A

E

D

A

E

G

F

A

A

D

G

W

V

V

I

M
x
K

P

M

G

G

F

R

T
|
T

H
|
H

L

L

Q

Q

T

D

A

A

Q

V

P

P

V

I

T

L

W

L

G

G

H

Q

H

E

M

F

M

E

A

A

Y

Y

V

A

E

R

M

V

F

I

G

A

R

R

D

D

L

I

S

E

R

R

V

I

R

A

D

N

A

T

R

R

T

N

R

N

M

L

N

Y

E

D

C

I

P

N

L

M

G

G

A

A

A

T

A

A

L

V

A

-

G

G

T

T

S

G

F

L

P

N

I

A

D

D

R

P

-

E

-

Y

-

I

S

S

M

I

T

V

A

T

E

E

A

H

L

L

G

A

-

K

F

F

E

S

-

G

-

H

-

P

-

L

R

R

P

S

A

A

A

Q

N

H

S

L

L

V

D

D

A

A

V

T

S

Q

D

N

R

T

D

D

F

C

A

Y

L

T

E

E

F

V

L

S

S

S

V

A

A

L

S

K

I

V

C

C

A

M

V

I

H

N

L

M

S

S

R

K

F

I

A

A

E

N

E

D

L

L

V

R

I

L

W

M

S

A

S

S

-

G

-

P

-

R

A

A

Q

G

F

L

R

S

F

E

V

I

T

V

L

L

S

P

D

A

R

R

F

-

S

Q

T

P

G

G

S
|
S

I
|
I

M
|
M

P
|
P

Q

G

K
|
K

K

V

N
|
N

P

P

D

V

A

M

A

P

E

E

L

V

I

M

R

N

A

Q

K

V

V

A

G

F

R

Q

I

V

F

F

G

G

T101 (vs. gap) binding L-aspartate; mutation to A: 7100-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to S: 80-fold decrease in catalytic efficiency.
H134 (≠ R107) mutation to A: Retains full activity. Shows a slightly stronger affinity for L-aspartate. Does not affect tertiary structure.
S140 (= S113) binding L-aspartate; mutation to A: 27-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation S->K,R: Loss of activity.
T141 (≠ R114) binding L-aspartate; mutation to A: 15-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to K: 40000-fold decrease in catalytic efficiency.; mutation T->V,R: Loss of activity.
N142 (= N115) binding L-aspartate; mutation to A: Loss of activity. Does not result in any major conformational changes.; mutation to Q: 3000-fold decrease in catalytic efficiency.
K183 (≠ M156) mutation to A: Loss of activity. Does not affect tertiary structure.
T187 (= T160) binding L-aspartate; mutation to A: 6280-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to S: 2.3-fold decrease in catalytic efficiency.
H188 (= H161) binding L-aspartate; mutation to A: 100-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation H->K,Q,R: Loss of activity.
S318 (= S282) mutation to A: Loss of activity.
S319 (= S283) binding L-aspartate; mutation to A: Almost no change in catalytic efficiency.
I320 (= I284) mutation to A: 50-fold decrease in catalytic efficiency.
M321 (= M285) mutation to A: 338-fold decrease in catalytic efficiency.
P322 (= P286) mutation to A: Almost no change in catalytic efficiency.
K324 (= K288) binding L-aspartate; mutation to A: Loss of activity. Does not result in any major conformational changes.; mutation K->D,H,R,S,V: Loss of activity.
N326 (= N290) mutation to A: 22500-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to Q: 168750-fold decrease in catalytic efficiency.

3r6qA A triclinic-lattice structure of aspartase from bacillus sp. Ym55-1 (see paper)
27% identity, 58% coverage: 40:306/464 of query aligns to 32:338/462 of 3r6qA

query
sites
3r6qA

I

I

Q

T

G

G

S

Y

R

R

A

I

H

H

A

P

A

E

M

L

L

I

A

K

A

S

S

L

G

G

V

I

I

V

T

K

D

K

S

S

D

A

A

A

E

L

A

A

I

N

R

M

E

E

-

V

G

G

L

L

T

-

I

-

L

-

S

-

E

D

I

K

E

E

A

V

G

G

N

Q

F

Y

Q

I

F

V

S

K

T

A

A

A

L

D

E

E

-

V

-

I

-

E

-

G

-

K

-

W

-

N

-

D

-

Q

-

F

-

I

-

V

-

D

-

P

-

I

-

Q

-

G

-

A

-

G

-

T

D

S

I

I

H

N

M

M

N

N

-

A

-

N

-

E

-

V

V

I

E

A

A

N

R

R

L

A

K

L

E

E

I

L

I

M

G

G

E

E

-

E

-

K

-

G

-

N

-

Y

-

S

-

K

-

I

-

S

P

P

A

N

G

S

R

H

L

V

H

N

T

M

G

S

R

Q

S

S

R

T

N

N

D

D

Q

A

V

F

A

P

T

T

D

A

F

T

K

H

L

I

W

A

V

V

R

L

D

S

Q

L

L

L

D

N

A

Q

A

L

E

I

T

E

G

T

L

T

L

K

A

Y

L

M

I

Q

A

Q

A

E

L

F

L

M

D

K

Q

K

A

A

E

D

A

E

G

F

A

A

D

G

W

V

V

I

M

K

P

M

G

G

F

R

T

T

H
|
H

L

L

Q

Q

T

D

A

A

Q

V

P

P

V

I

T

L

W

L

G

G

H

Q

H

E

M

F

M

E

A

A

Y

Y

V

A

E

R

M

V

F

I

G

A

R

R

D

D

L

I

S

E

R

R

V

I

R

A

D

N

A

T

R

R

T

N

R

N

M

L

N

Y

E

D

C

I

P

N

L

M

G

G

A

A

A

T

A

A

L

V

A

-

G

G

T

T

S

G

F

L

P

N

I

A

D

D

R

P

-

E

-

Y

-

I

S

S

M

I

T

V

A

T

E

E

A

H

L

L

G

A

-

K

F

F

E

S

-

G

-

H

-

P

-

L

R

R

P

S

A

A

A

Q

N

H

S

L

L

V

D

D

A

A

V

T

S

Q

D

N

R

T

D

D

F

C

A

Y

L

T

E

E

F

V

L

S

S

S

V

A

A

L

S

K

I

V

C

C

A

M

V

I

H

N

L

M

S

S

R

K

F

I

A

A

E

N

E

D

L

L

V

R

I

L

W

M

S

A

S

S

-

G

-

P

-

R

A

A

Q

G

F

L

R

S

F

E

V

I

T

V

L

L

S

P

D

A

R

R

F

-

S

Q

T

P

G

G

S
|
S

S

S

I

I

M

M

P

P

Q

G

K
|
K

K

V

N

N

P

P

D

V

A

M

A

P

E
|
E

L

V

I

M

R

N

A

Q

K

V

V

A

G

F

R

Q

I

V

F

F

G

G

active site: H184 (= H161), S314 (= S282), K320 (= K288), E327 (= E295)

Sites not aligning to the query:

binding calcium ion: 389, 391

P9WN93 Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase; EC 4.2.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
28% identity, 48% coverage: 90:311/464 of query aligns to 109:347/474 of P9WN93

query
sites
P9WN93

H

N

M

M

N

N

V

T

E

N

A

E

R

V

L

I

K

A

E

S

I

I

I

A

G

A

E

K

P

G

A

G

G

V

R

T

L

L

H

H

T

P

G

N

R

D

S

D

R

V

N

N

D

M

Q

S

V

Q

A
x
S

T
x
S

D
x
N

F

D

K

T

L

F

W

P

V

T

R

A

D

T

Q

H

L

I

D

A

A

A

A

T

E

E

T

A

G

A

L

V

L

A

A

H

L

L

I

I

A

P

A

A

L

L

L

Q

D

Q

Q

L

A

H

E

D

A

A

G

L

A

A

-

A

-

K

-

A

-

L

D

D

W

W

-

H

-

T

-

V

V

V

M

K

P

S

G

G

F

R

T
|
T

H

H

L

L

Q

M

T

D

A

A

Q

V

P

P

V

V

T

T

W

L

G

G

H

Q

H

E

M

F

M

S

A

G

Y

Y

V

A

E

R

M

Q

F

I

G

E

R

A

D

G

L

I

S

E

R

R

V

V

R

R

D

A

A

C

R

L

T

P

R

R

M

L

N

G

E

E

C

L

P

A

L

I

G

G

A

G

A

T

A

A

L

V

A

G

-

T

G

G

T

L

S

N

F

A

P

P

I

D

D

D

R

F

S

G

M

V

T

R

A

V

E

V

A

A

-

V

-

L

-

V

-

A

-

Q

-

T

-

G

L

L

G

S

F

E

E

L

R

R

P

T

A

A

N

N

S

S

L

F

D

E

A

A

V

Q

S

A

D

A

R

R

D

D

F

G

A

L

L

V

E

E

F

A

L

S

S

G

V

A

A

L

S

R

I

T

C

I

A

A

V

V

H

S

L

L

S

T

R

K

F

I

A

A

E

N

E

D

L

I

V

R

I

W

W

M

S

G

S

S

A

G

Q

P

F

L

R

T

F

G

-

L

-

A

-

E

V

I

T

Q

L

L

S

P

D

D

R

-

F

L

S

Q

T

P

G

G

S
|
S

I

I

M

M

P

P

Q

G

K
|
K

K
x
V

N
|
N

P

P

D

V

A

L

A

P

E

E

L

A

I

V

R

T

A

Q

K

V

V

A

G

A

R

Q

I

V

F

I

G

G

A

N

N

D

T

A

A

A

L

I

SSN 138:140 (≠ ATD 119:121) binding substrate
T186 (= T160) binding substrate
S318 (= S282) active site; mutation S->A,C: Absence of fumarase activity.
S319 (= S283) binding substrate
KVN 324:326 (≠ KKN 288:290) binding substrate

Sites not aligning to the query:

104:106 binding substrate

Query Sequence

>WP_090214906.1 NCBI__GCF_002796795.1:WP_090214906.1
MTDQTSNQMWGGRFAAGPDAIMEAINASIGFDKRMAAQDIQGSRAHAAMLAASGVITDSD
AEAIREGLLTILSEIEAGNFQFSTALEDIHMNVEARLKEIIGEPAGRLHTGRSRNDQVAT
DFKLWVRDQLDAAETGLLALIAALLDQAEAGADWVMPGFTHLQTAQPVTWGHHMMAYVEM
FGRDLSRVRDARTRMNECPLGAAALAGTSFPIDRSMTAEALGFERPAANSLDAVSDRDFA
LEFLSVASICAVHLSRFAEELVIWSSAQFRFVTLSDRFSTGSSIMPQKKNPDAAELIRAK
VGRIFGANTALMMVMKGLPLAYSKDMQEDKEQVFDAADNLMLALAAMEGMVKDMSANRES
LAAAAGSGFSTATDLADWLVRVLKVPFRDAHHVTGTLVGMAEARGCDLPDLSLDDMQGVH
SGITEDIFTVLGVENSVNSRTSYGGTAPEQVRAQVTRWKALLAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory