SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_090216759.1 NCBI__GCF_002796795.1:WP_090216759.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
70% identity, 98% coverage: 9:648/651 of query aligns to 6:642/648 of Q89WV5

query
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Q89WV5

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G263 (= G267) mutation to I: Loss of activity.
G266 (= G270) mutation to I: Great decrease in activity.
K269 (= K273) mutation to G: Great decrease in activity.
E414 (= E418) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
63% identity, 96% coverage: 21:648/651 of query aligns to 19:645/652 of P27550

query
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P27550

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K609 (= K612) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
63% identity, 96% coverage: 21:648/651 of query aligns to 19:645/652 of Q8ZKF6

query
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Q8ZKF6

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D

N

V

T

S

S

Y

F

D

A

F

P

G

G

N

N

V

V

G

S

I

I

K

K

W

W

Y

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

A

A

N

N

C

C

V

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

G

G

D

D

Q

R

T

T

A

A

I

I

W

W

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G

D

D

P

T

K

S

D

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-

A

S

Q

K

H

H

I

I

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Y

Y

K

R

D

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L

L

H

H

T

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S

D

V

V

C

C

K

R

M

F

A

A

N

N

V

T

L

L

E

L

T

D

L

L

G

G

V

I

S

K

K

K

G

G

D

D

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V

V

V

V

A

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I

Y

Y

M

M

P

P

M

M

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A

A

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A

A

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M

L

L

A

A

C

C

A

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G

A

A

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H

H

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V

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F

A

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G

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V

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I

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A

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D

D

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A

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V

R

R

G

A

G

G

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x
R

K

S

T

I

P

P

L

L

K

K

T

K

N

N

A

V

D

D

A

D

A

A

L

L

S

K

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N

-

P

-

N

C

V

A

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V

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K

R

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G

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W

N

Q

D

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A

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D

D

R

L

D

W

Y

W

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K

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L

L

S

I

E

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G

K

A

A

N

S

L

P

T

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H

H

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P

P

A

E

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A

M

M

N

N

A

A

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D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

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G

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P

P

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K

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H

H

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Y

L

L

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V

Y

Y

A

A

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F

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Y

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V

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F

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D

Y

Y

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H

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G

G

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V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

V

L

M

M

F

F

E

E

G

G

V

V

P

P

T
x
N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

Q

Q

V

V

C

V

E

D

K

K

H

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

G

A

Q

E

G

G

N

D

E

K

F

A

V

I

E

E

K

G

C

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

N

W

D

K

I

K

V

I

G

G

G

K

G

E

R

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

H

F

L

M

M

I

T

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

T

L

K

K

P

A

G

G

S

S

A

A

M

T

K

R

P

P

F

F

G

G

I

V

E

Q

P

P

V

A

V

L

L

V

D

D

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-

A

N

S

E

G

G

D

H

E

P

I

Q

T

E

G

G

N

A

D

-

V

T

E

E

G

G

V

N

L

L

C

V

I

I

K

T

D

D

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S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

V

L

W

F

G

G

D

D

H

H

D

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R

R

F

F

E

E

K

Q

T

T

Y

Y

F

F

S

S

D

T

Y

F

K

K

G

N

Y

M

Y

Y

F

F

T

S

G

G

D

D

G

G

C

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I

I

T

T

G

G

R

R

V

V

D
|
D

D

D

V

V

I

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

A

A

H

H

A

P

T

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

N

H

G

G

E

E

T

E

P

P

S

S

E

P

E

E

L

L

R

Y

K

A

E

E

L

V

R

R

T

N

W

W

V

V

R
|
R

S

K

E

E

I

I

G

G

P

P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

T

G

S

A

N

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

V

V

D

E

D

K

L

L

I

L

A

E

N

E

R

K

R194 (≠ K197) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T312) binding CoA
N335 (≠ T336) binding CoA
A357 (= A358) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D520) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S526) binding CoA
G524 (= G527) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R529) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R587) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K612) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
63% identity, 96% coverage: 21:648/651 of query aligns to 15:638/640 of 5jrhA

query
sites
5jrhA

V

I

D

N

A

P

A

E

K

Q

Y

Y

D

E

V

T

L

K

Y

Y

A

K

A

Q

S

S

I

I

E

N

D

D

P

P

E

D

G

T

F

F

W

W

R

G

E

E

Q

Q

G

G

K

K

R

I

V

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

Q

K

V

V

K

K

D

N

V

T

S

S

Y

F

D

A

F

P

G

G

N

N

V

V

G

S

I

I

K

K

W

W

Y

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

A

A

N

N

C

C

V

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

G

G

D

D

Q

R

T

T

A

A

I

I

W
|
W

E

E

P

G

D

D

P

T

K

S

D

Q

P

-

A

S

Q

K

H

H

I

I

T

S

Y

Y

K

R

D

E

L

L

H

H

T

R

S

D

V

V

C

C

K

R

M

F

A

A

N

N

V

T

L

L

E

L

T

D

L

L

G

G

V

I

S

K

K

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E
|
E

A

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

I

V

H

H

S

S

I

V

V

I

F
|
F

A
x
G

G
|
G

F

F

S

S

P

P

D

E

A

A

L

V

A

A

A
x
G

R

R

I

I

N

I

G

D

C

S

D

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

E

E

A

G

P

V

R
|
R

G

A

G

G

K

R

K

S

T

I

P

P

L

L

K

K

T

K

N

N

A

V

D

D

A

D

A

A

L

L

S

K

Q

N

-

P

-

N

C

V

A

T

D

S

A

V

V

E

Q

H

C

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

G

S

D

D

V

I

P

D

W

W

N

Q

D

E

A

G

R

R

D

D

R

L

D

W

Y

W

S

R

K

D

L

L

S

I

E

E

G

K

A

A

N

S

L

P

T

E

H

H

T

Q

P

P

A

E

E

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

Q
x
K

P
|
P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

L

T

T

T

Q

F

E

K

V

Y

T

V

F

F

D

D

Y

Y

H

H

D

P

G

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

V

L

M

M

F

F

E

E

G

G

V

V

P

P

T

N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

Q

Q

V

V

C

V

E

D

K

K

H

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

G

A

Q

E

G

G

N

D

E

K

F

A

V

I

E

E

K

G

C

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

N

W

D

K

I

K

V

I

G

G

G

K

G

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

L

M

M

I

T

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

T

L

K

K

P

A

G

G

S

S

A

A

M

T

K

R

P

P

F

F

G

G

I

V

E

Q

P

P

V

A

V

L

L

V

D

D

P

-

A

N

S

E

G

G

D

H

E

P

I

Q

T

E

G

G

N

A

D

-

V

T

E

E

G

G

V

N

L

L

C

V

I

I

K

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

V

L

W

F

G

G

D

D

H

H

D

E

R

R

F

F

E

E

K

Q

T

T

Y

Y

F

F

S

S

D

T

Y

F

K

K

G

N

Y

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

A

A

H
|
H

A

P

T

K

V
x
I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

N

H

G

G

E

E

T

E

P

P

S

S

E

P

E

E

L

L

R

Y

K

A

E

E

L

V

R

R

T

N

W

W

V

V

R
|
R

S

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

-

G

-

A

-

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

V

V

D

E

D

K

L

L

I

L

A

E

N

E

R

K

active site: T260 (= T265), T412 (= T417), E413 (= E418), N517 (= N524), R522 (= R529), K605 (= K612)
binding (r,r)-2,3-butanediol: W93 (= W99), E140 (= E147), G169 (≠ A176), K266 (≠ Q271), P267 (= P272)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G388), E384 (= E389), P385 (= P390), T408 (= T413), W409 (= W414), W410 (= W415), Q411 (= Q416), T412 (= T417), D496 (= D503), I508 (= I515), N517 (= N524), R522 (= R529)
binding coenzyme a: F159 (= F166), G160 (≠ A167), G161 (= G168), R187 (= R194), S519 (= S526), R580 (= R587), P585 (= P592)
binding magnesium ion: V533 (= V540), H535 (= H542), I538 (≠ V545)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
62% identity, 96% coverage: 21:648/651 of query aligns to 15:639/641 of 2p20A

query
sites
2p20A

V

I

D

N

A

P

A

E

K

Q

Y

Y

D

E

V

T

L

K

Y

Y

A

K

A

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S

S

I

I

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N

D

D

P

P

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D

G

T

F

F

W

W

R

G

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E

Q

Q

G

G

K

K

R

I

V

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

Q

K

V

V

K

K

D

N

V

T

S

S

Y

F

D

A

F

P

G

G

N

N

V

V

G

S

I

I

K

K

W

W

Y

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

A

A

N

N

C

C

V

L

D

D

R

R

H

H

L

L

A

Q

K

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G

G

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D

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T

T

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A

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I

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W

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D

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A

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H

H

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L

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V

C

C

K

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L

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L

L

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G

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K

K

G

G

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D

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V

V

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I

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Y

M

M

P

P

M

M

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P

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A

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

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V

H

H

S

S

I

V

V

I

F

F

A

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G

G

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F

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S

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P

D

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A

A

L

V

A

A

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R

R

I

I

N

I

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C

S

D

S

A

S

K

R

L

L

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V

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I

T

T

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A

D

D

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A

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P

V

R

R

G

A

G

G

K

R

K

S

T

I

P

P

L

L

K

K

T

K

N

N

A

V

D

D

A

D

A

A

L

L

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K

Q

N

-

P

-

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C

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A

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V

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C

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L

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K

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G

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V

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W

N

Q

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R

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D

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D

W

Y

W

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L

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K

A

A

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S

L

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T

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H

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P

P

A

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M

M

N

N

A

A

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E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

Q

K

P

P

K

K

G

G

V

V

V

L

H

H

T

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G

G

Y

Y

L

L

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V

Y

Y

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A

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A

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F

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K

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F

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D

Y

Y

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H

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G

G

D

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I

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Y

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W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

V

L

M

M

F

F

E

E

G

G

V

V

P

P

T

N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

Q

Q

V

V

C

V

E

D

K

K

H

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

G

A

Q

E

G

G

N

D

E

K

F

A

V

I

E

E

K

G

C

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

N

W

D

K

I

K

V

I

G

G

G

K

G

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

L

M

M

I

T

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

T

L

K

K

P

A

G

G

S

S

A

A

M

T

K

R

P

P

F

F

G

G

I

V

E

Q

P

P

V

A

V

L

L

V

D

D

P

-

A

N

S

E

G

G

D

H

E

P

I

Q

T

E

G

G

N

A

D

-

V

T

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E

G

G

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N

L

L

C

V

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D

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W

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P

G

G

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Q

M

A

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L

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G

G

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D

H

H

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R

F

F

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K

Q

T

T

Y

Y

F

F

S

S

D

T

Y

F

K

K

G

N

Y

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

A

A

H

H

A

P

T

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

N

H

G

G

E

E

T

E

P

P

S

S

E

P

E

E

L

L

R

Y

K

A

E

E

L

V

R

R

T

N

W

W

V

V

R

A

S

K

E

E

I

I

G

G

P

P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

T

G

S

A

-

L

-

G

N

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

S

G

V

V

D

E

D

K

L

L

I

L

A

E

N

E

R

K

active site: T260 (= T265), T412 (= T417), E413 (= E418), N517 (= N524), R522 (= R529), K605 (= K612)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G388), E384 (= E389), P385 (= P390), T408 (= T413), W409 (= W414), W410 (= W415), Q411 (= Q416), T412 (= T417), D496 (= D503), I508 (= I515), R511 (= R518), R522 (= R529)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
62% identity, 96% coverage: 21:648/651 of query aligns to 14:635/637 of 2p2fA

query
sites
2p2fA

V

I

D

N

A

P

A

E

K

Q

Y

Y

D

E

V

T

L

K

Y

Y

A

K

A

Q

S

S

I

I

E

N

D

D

P

P

E

D

G

T

F

F

W

W

R

G

E

E

Q

Q

G

G

K

K

R

I

V

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

Q

K

V

V

K

K

D

N

V

T

S

S

Y

F

D

A

F

P

G

G

N

N

V

V

G

S

I

I

K

K

W

W

Y

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

A

A

N

N

C

C

V

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

G

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

P

G

D

D

P

T

K

S

D

Q

P

-

A

S

Q

K

H

H

I

I

T

S

Y

Y

K

R

D

E

L

L

H

H

T

R

S

D

V

V

C

C

K

R

M

F

A

A

N

N

V

T

L

L

E

L

T

D

L

L

G

G

V

I

S

K

K

K

G

G

D

D

R

V

V

V

V

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

I

V

H

H

S

S

I

V

V

I

F
|
F

A

G

G

G

F

F

S

S

P

P

D

E

A

A

L

V

A

A

A

G

R

R

I

I

N

I

G

D

C

S

D

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

E

E

A

G

P

V

R
|
R

G

A

G

G

K

R

K

S

T

I

P

P

L

L

K

K

T

K

N

N

A

V

D

D

A

D

A

A

L

L

S

K

Q

N

-

P

-

N

C

V

A

T

D

S

A

V

V

E

Q

H

C

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

G

S

D

D

V

I

P

D

W

W

N

Q

D

E

A

G

R

R

D

D

R

L

D

W

Y

W

S

R

K

D

L

L

S

I

E

E

G

K

A

A

N

S

L

P

T

E

H

H

T

Q

P

P

A

E

E

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

Q

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

L

T

T

T

Q

F

E

K

V

Y

T

V

F

F

D

D

Y

Y

H

H

D

P

G

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W
|
W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

V

L

M

M

F

F

E

E

G

G

V

V

P
|
P

T

N

Y

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

Q

Q

V

V

C

V

E

D

K

K

H

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

M

M

G

A

Q

E

G

G

N

D

E

K

F

A

V

I

E

E

K

G

C

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

N

W

D

K

I

K

V

I

G

G

G

K

G

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

L

M

M

I

T

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

T

L

K

K

P

A

G

G

S

S

A

A

M

T

K

R

P

P

F

F

G

G

I

V

E

Q

P

P

V

A

V

L

L

V

D

D

P

-

A

N

S

E

G

G

D

H

E

P

I

Q

T

E

G

G

N

A

D

-

V

T

E

E

G

G

V

N

L

L

C

V

I

I

K

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

V

L

W

F

G

G

D

D

H

H

D

E

R

R

F

F

E

E

K

Q

T

T

Y

Y

F

F

S

S

D

T

Y

F

K

K

G

N

Y

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

A

A

H

H

A

P

T

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

N

H

G

G

E

E

T

E

P

P

S

S

E

P

E

E

L

L

R

Y

K

A

E

E

L

V

R

R

T

N

W

W

V

V

R
|
R

S

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

Y

-

G

-

A

-

L

-

G

-

D

T

T

S

S

S

T

T

L

L

A

A

D

D

P

P

S

G

V

V

D

E

D

K

L

L

I

L

A

E

N

E

R

K

active site: T259 (= T265), T411 (= T417), E412 (= E418), N516 (= N524), R521 (= R529), K604 (= K612)
binding adenosine monophosphate: G382 (= G388), E383 (= E389), P384 (= P390), T407 (= T413), W408 (= W414), W409 (= W415), Q410 (= Q416), T411 (= T417), D495 (= D503), I507 (= I515), R510 (= R518), N516 (= N524), R521 (= R529)
binding coenzyme a: F158 (= F166), R186 (= R194), W304 (= W310), T306 (= T312), P329 (= P335), A352 (= A358), A355 (= A361), S518 (= S526), R579 (= R587), P584 (= P592)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
61% identity, 96% coverage: 21:648/651 of query aligns to 15:632/634 of 1pg3A

query
sites
1pg3A

V

I

D

N

A

P

A

E

K

Q

Y

Y

D

E

V

T

L

K

Y

Y

A

K

A

Q

S

S

I

I

E

N

D

D

P

P

E

D

G

T

F

F

W

W

R

G

E

E

Q

Q

G

G

K

K

R

I

V

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

Q

K

V

V

K

K

D

N

V

T

S

S

Y

F

D

A

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active site: T260 (= T265), T412 (= T417), E413 (= E418), N517 (= N524), R522 (= R529), K605 (= K612)
binding coenzyme a: F159 (= F166), G160 (≠ A167), R187 (= R194), R190 (≠ K197), A301 (= A306), T307 (= T312), P330 (= P335), A356 (= A361), S519 (= S526), R580 (= R587), P585 (= P592)
binding magnesium ion: V533 (= V540), H535 (= H542), I538 (≠ V545)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G388), E384 (= E389), P385 (= P390), T408 (= T413), W409 (= W414), W410 (= W415), Q411 (= Q416), T412 (= T417), D496 (= D503), R511 (= R518), R522 (= R529)

Q9NR19 Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 from Homo sapiens (Human) (see paper)
53% identity, 99% coverage: 8:651/651 of query aligns to 28:699/701 of Q9NR19

query
sites
Q9NR19

T
x
S

Y
x
W

T
x
S

P
|
P

T
x
P

E
x
P

D
x
E

F
x
V

V
x
S

Q
x
R

N
x
S

A
|
A

H
|
H

V
|
V

D
x
P

A
x
S

-
x
L

A
x
Q

K
x
R

Y
|
Y

D
x
R

V
x
E

L
|
L

Y
x
H

A
x
R

S
|
S

I
x
V

E
|
E

D
x
E

P
|
P

E
x
R

G
x
E

F
|
F

W
|
W

R
x
G

E
x
D

Q
x
I

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x
A

K
|
K

R
x
E

V
x
F

D
x
Y