SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_090216766.1 NCBI__GCF_002796795.1:WP_090216766.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
44% identity, 61% coverage: 3:203/330 of query aligns to 68:279/482 of O59954

query
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O59954

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G82 (= G17) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (≠ A35) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R65) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (= C120) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ G150) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G188) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
39% identity, 69% coverage: 1:228/330 of query aligns to 69:299/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
29% identity, 93% coverage: 3:309/330 of query aligns to 46:413/440 of P38820

query
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P38820

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C225 (= C177) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.
C397 (= C293) mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 71% coverage: 1:235/330 of query aligns to 9:245/249 of P12282

query
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P12282

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R14 (= R6) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ A36) mutation to A: No effect.
R73 (= R65) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (= C120) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D122) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C134) mutation to A: No effect.
C172 (≠ S162) mutation to A: No zinc bound and no enzyme activity.
C175 (≠ A165) mutation to A: No zinc bound and no enzyme activity.
C187 (= C177) mutation to A: No effect.
C231 (≠ F221) mutation to A: No effect.
C244 (≠ P234) mutation to A: No zinc bound and almost no enzyme activity.

Sites not aligning to the query:

247 C→A: No zinc bound and almost no enzyme activity.

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
37% identity, 62% coverage: 1:205/330 of query aligns to 12:215/289 of 6yubB

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6yubB

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D

C

C

A

T

D

D

S

N

F

P

A

A

A

T

S

R

Y

Y

I

L

A

I

S

S

D

D

I

V

C

C

G

V

K

L

R

L

N

G

I

K

P

P

L

L

I

V

S

S

A

A

S

A

V

S

V

V

E

Q

M

K

G

S

G

G

Y

Q

V

L

G

I

G

V

F

L

C

N

A

C

G

-

A

P

P

P

S

T

L

P

R

Q

A

G

V

V

F

V

P

N

D

K

L

K

P

A

R

A

A

P

S
x
C

G

Y

T

R

C
|
C

A

C

T

F

L

K

G

G

V

I

L

M

G

G

P

P

I

V

V

V

G

G

I

M

A

G

T

V

L

A

Q

Q

A

A

Q

G

M

E

A

A

I

I

S

K

V

I

L

L

T

V

G

S

Q

Q

binding zinc ion: C184 (≠ S174), C187 (= C177)

Sites not aligning to the query:

binding zinc ion: 265

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
36% identity, 71% coverage: 1:235/330 of query aligns to 8:237/240 of 1jwbB

query
sites
1jwbB

M

M

S

L

R

R

Y

Y

A

N

R
|
R

Q

Q

I

I

A

I

V

L

S

R

E

G

F

F

G

D

P

F

H

D

G

G

Q

Q

E

E

A

A

L

L

R

K

H

D

A

S

R

R

L

V

L

L

V

I

I

V

G
|
G

A

L

G
|
G

L

L

A

G

A

C

P

A

V

A

L

S

P

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

I

L

K

T

L

L

A

L

D
|
D

P
x
F

D

D

I

T

V

V

D

S

L

L

S

S

N

N

L

L

H

Q

R

R

Q

Q

T

T

L

L

F

H

T

S

M

D

D

A

D

T

I

V

G

G

L

Q

S

P

K
|
K

A

V

T

E

S

S

A

A

A

R

K

D

H

A

L

L

S

T

A

R

R

I

N

N

P

P

E

H

C

I

L

A

I

I

E

T

T

P

H

V

V

N

A

A

P
x
L

F

L

D

D

P

D

D

A

T

E

A

L

H

A

D

A

L

L

C

I

N

A

D

E

I

H

D

D

L

L

V

V

L

L

D

D

C
|
C

A
x
T

D
|
D

S
x
N

F

V

A

A

A
x
V

S

R

Y

N

I

Q

A

L

S

N

D

A

I

G

C

C

G

F

K

A

R

A

N

K

I

V

P

P

L

L

I

V

S

S

A

G

S

A

V

A

V

I

E

R

M

M

G

E

G

G

Y

Q

V

I

G

T

G

V

F

F

C

T

A

Y

-

Q

-

D

G

G

A

E

P

P

S
x
C

L

Y

R

R

A

C

V

-

F

-

P

-

D

-

L

L

P

S

R

R

A

L

S

F

G

G

T

E

C

A

A

-

T

-

L

-

G

G

V

V

L

M

G

A

P

P

I

L

V

I

G

G

I

V

I

I

A

G

T

S

L

L

Q

Q

A

A

Q

M

M

E

A

A

I

I

S

K

V

M

L

L

T

A

G

G

Q

Y

S

G

P

K

S

P

P

A

L

S

G

G

Q

K

L

I

I

V

T

M

F

Y

E

D

G

A

T

M

G

T

F

C

R

Q

F

F

G

R

G

E

F

M

R

K

F

L

D

M

T

R

A

N

P

P

E

G

P
x
C

E

E

active site: R13 (= R6), D129 (= D122)
binding adenosine monophosphate: G37 (= G30), G39 (= G32), G40 (= G33), D61 (= D54), F62 (≠ P55), K85 (= K78), L108 (≠ P101), C127 (= C120), T128 (≠ A121), D129 (= D122), N130 (≠ S123), V133 (≠ A126)
binding zinc ion: C171 (≠ S162), C236 (≠ P234)

Sites not aligning to the query:

binding zinc ion: 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
36% identity, 71% coverage: 1:235/330 of query aligns to 8:237/240 of 1jw9B

query
sites
1jw9B

M

M

S

L

R

R

Y

Y

A

N

R
|
R

Q

Q

I

I

A

I

V

L

S

R

E

G

F

F

G

D

P

F

H

D

G

G

Q

Q

E

E

A

A

L

L

R

K

H

D

A

S

R

R

L

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

A

G

A

C

P

A

V

A

L

S

P

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

I

L

K

T

L

L

A

L

D

D

P

F

D

D

I

T

V

V

D

S

L

L

S

S

N

N

L

L

H

Q

R

R

Q

Q

T

T

L

L

F

H

T

S

M

D

D

A

D

T

I

V

G

G

L

Q

S

P

K

K

A

V

T

E

S

S

A

A

A

R

K

D

H

A

L

L

S

T

A

R

R

I

N

N

P

P

E

H

C

I

L

A

I

I

E

T

T

P

H

V

V

N

A

A

P

L

F

L

D

D

P

D

D

A

T

E

A

L

H

A

D

A

L

L

C

I

N

A

D

E

I

H

D

D

L

L

V

V

L

L

D

D

C

C

A

T

D
|
D

S

N

F

V

A

A

A

V

S

R

Y

N

I

Q

A

L

S

N

D

A

I

G

C

C

G

F

K

A

R

A

N

K

I

V

P

P

L

L

I

V

S

S

A

G

S

A

V

A

V

I

E

R

M

M

G

E

G

G

Y

Q

V

I

G

T

G

V

F

F

C

T

A

Y

-

Q

-

D

G

G

A

E

P

P

S
x
C

L

Y

R

R

A

C

V

-

F

-

P

-

D

-

L

L

P

S

R

R

A

L

S

F

G

G

T

E

C

A

A

-

T

-

L

-

G

G

V

V

L

M

G

A

P

P

I

L

V

I

G

G

I

V

I

I

A

G

T

S

L

L

Q

Q

A

A

Q

M

M

E

A

A

I

I

S

K

V

M

L

L

T

A

G

G

Q

Y

S

G

P

K

S

P

P

A

L

S

G

G

Q

K

L

I

I

V

T

M

F

Y

E

D

G

A

T

M

G

T

F

C

R

Q

F

F

G

R

G

E

F

M

R

K

F

L

D

M

T

R

A

N

P

P

E

G

P
x
C

E

E

active site: R13 (= R6), D129 (= D122)
binding zinc ion: C171 (≠ S162), C236 (≠ P234)

Sites not aligning to the query:

binding zinc ion: 239

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
35% identity, 71% coverage: 1:233/330 of query aligns to 10:244/270 of D4GSF3

query
sites
D4GSF3

M

L

S

D

R

R

Y

Y

A

S

R

R

Q

H

I

I

A

I

V

M

S

D

E

E

F

V

G

G

P

P

H

E

G

G

Q

Q

E

G

A

R

L

L

R

L

H

S

A

S

R

R

L

V

V

V

I

V

G

G

A

A

G

G

L

L

A

G

A

A

P

P

V

A

L

I

P

Q

Y

Y

L

L

A

A

A

V

G

G

V

V

G

G

T

E

I

L

K

V

L

V

A

V

D

D

P

D

D

D

I

V

V

V

D

E

L

R

S

S

N

N

L

L

H

Q

R

R

Q

Q

T

V

L

V

F

H

T

C

M

D

D

D

I

V

G

G

L

T

S

P

K

K

A

A

T

E

S

S

A

A

K

A

H

F

L

V

S

R

A

G

R

L

N

N

P

P

E

D

C

V

L

S

I

V

E

E

T

P

H

V

V

E

A

A

P

R

F

V

D

D

P

K

D

S

T

N

A

V

H

H

D

E

L

V

C

V

N

A

D

G

I

S

D

D

L

V

V

V

L

V

D

D

C

A

A

S

D

D

S

N

F

F

A

P

A

T

S

R

Y

Y

I

L

A

L

S

N

D

D

I

V

C

C

G

R

K

F

R

E

N

G

I

I

P

P

L

L

I

V

S

H

A

G

S

A

V

I

V

Y

E

K

M

F

G

E

G

G

Y

Q

V

A

G

T

F

L

C

V

A

P

G

D

A

G

P

P

S

C

L

Y

R

R

A

C

V

L

F

F

P

P

D

E

L

A

P

P

R

E

A

P

S

G

G

T

T

V

-

P

-

D

C
|
C

A

A

T

T

L

T

G

G

V

V

L

L

G

G

P

V

I

L

V

P

G

G

I

T

I

V

A

G

T

C

L

I

Q

Q

A

A

Q

T

M

E

A

A

I

M

S

K

V

L

L

L

T

L

G

D

Q

E

S

G

P

E

S

A

P

L

L

D

G

G

Q

R

L

L

I

L

T

F

F

Y

E

D

G

A

T

M

G

D

F

M

R

T

F

F

G

E

G

T

F

V

R

P

F

Y

D

R

T

T

A

N

P

P

E

D

C188 (= C177) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
36% identity, 62% coverage: 1:205/330 of query aligns to 11:216/423 of 6yubA

query
sites
6yubA

M

I

S

E

R

R

Y

Y

A

S

R

R

Q

Q

I

M

A

I

V

V

S

P

E

G

F

M

G

G

P

K

H

E

G

G

Q

Q

E

L

A

R

L

L

R

M

H

N

A

A

R

K

L

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

A

G

A

C

P

P

V

A

L

A

P

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

I

K

G

L

I

A

V

D

D

P

G

D

D

I

S

V

V

D

E

L

T

S

S

N

N

L

L

H

H

R

R

Q

Q

T

V

L

A

F

H

T

A

M

T

D

K

D

R

I

V

G

G

L

M

S

L

K

K

A

V

T

D

S

S

A

L

A

I

K

T

H

H

L

L

S

I

A

E

R

I

N

N

P

P

E

L

C

P

L

V

I

Y

E

V

T

P

H

Y

V

R

A

F

P

D

F

L

D

T

P

P

D

Q

T

N

A

A

H

A

D

Q

L

I

C

I

N

K

D

P

I

W

D

D

L

V

V

I

L

L

D

D

C

C

A

T

D

D

S

N

F

P

A

A

A

T

S

R

Y

Y

I

L

A

I

S

S

D

D

I

V

C

C

G

V

K

L

R

L

N

G

I

K

P

P

L

L

I

V

S

S

A

A

S

A

V

S

V

V

E

Q

M

K

G

S

G

G

Y

Q

V

L

G

I

G

V

F

L

-

N

C

C

A

P

G

P

A

T

P

P

S

Q

L

G

R

V

A

V

V

N

F

K

P

K

D

A

L

A

P

P

R
x
C

A

Y

S

R

G
x
C

T

C

C

F

A

K

T

K

L

P

G

G

V

I

L

M

G

G

P

P

I

V

V

V

G

G

I

M

A

G

T

V

L

A

Q

Q

A

A

Q

G

M

E

A

A

I

I

S

K

V

I

L

L

T

V

G

S

Q

Q

binding zinc ion: C183 (≠ R172), C186 (≠ G175)

Sites not aligning to the query:

binding zinc ion: 266, 269

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
38% identity, 67% coverage: 3:223/330 of query aligns to 62:285/460 of O95396

query
sites
O95396

R

R

Y

Y

A

S

R

R

Q

Q

I

L

A

V

V

L

S

P

E

E

F

L

G

G

P

V

H

H

G

G

Q

Q

E

L

A

R

L

L

R

G

H

T

A

A

R

C

L

V

L

L

V

I

I

V

G

G

A

C

G

G

L

L

A

G

A

C

P

P

V

L

L

A

P

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

T

R

I

L

K

G

L

L

A

V

D

D

P

Y

D

D

I

V

V

V

D

E

L

M

S

S

N

N

L

L

H

A

R

R

Q

Q

T

V

L

L

F

H

T

G

M

E

D

A

D

L

I

A

G

G

L

Q

S

A

K

K

A

A

T

F

S

S

A

A

K

A

H

S

L

L

S

R

A

R

R

L

N

N

P

S

E

A

C

V

L

E

I

C

E

V

T

P

H

Y

V
x
T

A
x
Q

P
x
A

F
x
L

D
x
T

P
|
P

D
x
A

T
|
T

A
|
A

H
x
L

D
|
D

L
|
L

C
x
V

N
x
R

D
x
R

I
x
Y

D
|
D

L
x
V

V
|
V

L
x
A

D
|
D

C
|
C

A
x
S

D
|
D

S
x
N

F
x
V

A
x
P

A
x
T

S
x
R

Y
|
Y

I
x
L

A
x
V

S
x
N

D
|
D

I
x
A

C
|
C

G
x
V

K
x
L

R
x
A

N
x
G

I
x
R

P
|
P

L
|
L

I
x
V

S
|
S

A
|
A

S
|
S

V
x
A

V
x
L

E
x
R

M
x
F

G
x
E

G
|
G

Y
x
Q

V
x
I

G
x
T

G
x
V

F
x
Y

-
x
H

C
x
Y

A
x
D

G
|
G

A
x
G

P
|
P

S
x
C

L
x
Y

R
|
R

A
x
C

V
x
I

F
|
F

P
|
P

D
x
Q

L
x
P

P
|
P

R
x
P

A
|
A

S
x
E

-
x
T

-
x
V

G
x
T

T
x
N

C
|
C

A

A

T

D

L

G

G

G

V

V

L

L

G

G

P

V

I

V

V

T

G

G

I

V

I

L

A

G

T

C

L

L

Q

Q

A

A

Q

L

M

E

A

V

I

L

S

K

V

I

L

A

T

A

G

G

Q

L

S

G

P

P

S

S

P

Y

L

S

G

G

Q

S

L

L

I

L

T

L

F

F

E

D

G

A

T

L

G

R

F

G

R

H

F

F

158:238 (vs. 99:176, 32% identical) Interaction with NFS1
C239 (= C177) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

1zfnA Structural analysis of escherichia coli thif (see paper)
38% identity, 67% coverage: 3:223/330 of query aligns to 8:229/244 of 1zfnA

query
sites
1zfnA

R

R

Y

Y

A

S

R
|
R

Q

Q

I

I

A

L

V

L

S

D

E

D

F

I

G

A

P

L

H

D

G

G

Q

Q

E

Q

A

K

L

L

R

L

H

D

A

S

R

Q

L

V

L

L

V

I

I
|
I

G
|
G

A

L

G
|
G

L

L

A

G

A

T

P

P

V

A

L

A

P

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

L

K

V

L

L

A

A

D
|
D

P

D

D

D

I

D

V

V

D

H

L

L

S

S

N

N

L

L

H

Q

R
|
R

Q
|
Q

T

I

L

L

F

F

T

T

M

T

D

E

D

D

I

I

G

D

L

R

S

P

K
|
K

A

S

T

Q

S

V

A

S

A

Q

K

Q

H

R

L

L

S

T

A

Q

R

L

N

N

P

P

E

D

C

I

L

Q

I

L

E

T

T

A

H

L

V

Q

A

Q

P

R

F

L

D

T

P

G

D

E

T

A

A

L

H

K

D

D

L

A

C

V

N

A

D

R

I

A

D

D

L

V

V

V

L

L

D

D

C

C

A
x
T

D
|
D

S

N

F

M

A

A

A
x
T

S

R

Y

Q

I

E

A

I

S

N

D

A

I

A

C

C

G

V

K

A

R

L

N

N

I

T

P

P

L

L

I

I

S

T

A

A

S

S

V

A

V

V

E

G

M

F

G

G

Y

Q

V

L

G

M

G

V

F

L

C

T

-

P

A

W

G

E

A

Q

P

G

S
x
C

L

Y

R

R

A
x
C

V

L

F

W

P

P

D

D

L

N

P

Q

R

E

A

P

S

E

G

R

T

N

C

C

A

R

T

T

L

A

G

G

V

V

L

V

G

G

P

P

I

V

V

V

G

G

I

V

I

M

A

G

T

T

L

L

Q

Q

A

A

Q

L

M

E

A

A

I

I

S

K

V

L

L

L

T

S

G

G

Q

-

S

I

P

E

S

T

P

P

L

A

G

G

Q

E

L

L

I

R

T

L

F

F

E

D

G

G

T

K

G

S

F

S

R

Q

F

W

active site: R11 (= R6), D127 (= D122)
binding adenosine-5'-triphosphate: I34 (= I29), G35 (= G30), G37 (= G32), G38 (= G33), D59 (= D54), R70 (= R65), Q71 (= Q66), K83 (= K78), T126 (≠ A121), D127 (= D122), T131 (≠ A126)
binding zinc ion: C169 (≠ S162), C172 (≠ A165)

Sites not aligning to the query:

binding zinc ion: 240, 243

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 67% coverage: 3:223/330 of query aligns to 8:229/251 of P30138

query
sites
P30138

R

R

Y

Y

A

S

R

R

Q

Q

I

I

A

L

V

L

S

D

E

D

F

I

G

A

P

L

H

D

G

G

Q

Q

E

Q

A

K

L

L

R

L

H

D

A

S

R

Q

L

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

A

G

A

T

P

P

V

A

L

A

P

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

L

K

V

L

L

A

A

D

D

P

D

D

D

I

D

V

V

D

H

L

L

S

S

N

N

L

L

H

Q

R

R

Q

Q

T

I

L

L

F

F

T

T

M

T

D

E

D

D

I

I

G

D

L

R

S

P

K

K

A

S

T

Q

S

V

A

S

A

Q

K

Q

H

R

L

L

S

T

A

Q

R

L

N

N

P

P

E

D

C

I

L

Q

I

L

E

T

T

A

H

L

V

Q

A

Q

P

R

F

L

D

T

P

G

D

E

T

A

A

L

H

K

D

D

L

A

C

V

N

A

D

R

I

A

D

D

L

V

V

V

L

L

D

D

C

C

A

T

D

D

S

N

F

M

A

A

A

T

S

R

Y

Q

I

E

A

I

S

N

D

A

I

A

C

C

G

V

K

A

R

L

N

N

I

T

P

P

L

L

I

I

S

T

A

A

S

S

V

A

V

V

E

G

M

F

G

G

Y

Q

V

L

G

M

G

V

F

L

C

T

A

P

G

P

A

W

P

E

S

Q

-

G

-
x
C

L

Y

R

R

A
x
C

V

L

F
x
W

P

P

D

D

L

N

P

Q

R

E

A

P

S

E

G

R

T

N

C
|
C

A

R

T

T

L

A

G

G

V

V

L

V

G

G

P

P

I

V

V

V

G

G

I

V

I

M

A

G

T

T

L

L

Q

Q

A

A

Q

L

M

E

A

A

I

I

S

K

V

L

L

L

T

S

G

G

Q

-

S

I

P

E

S

T

P

P

L

A

G

G

Q

E

L

L

I

R

T

L

F

F

E

D

G

G

T

K

G

S

F

S

R

Q

F

W

C169 (vs. gap) binding Zn(2+)
C172 (≠ A165) binding Zn(2+)
W174 (≠ F167) mutation to A: No adenylation of ThiS.
C184 (= C177) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.

Sites not aligning to the query:

240 binding Zn(2+)
243 binding Zn(2+)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
35% identity, 68% coverage: 1:223/330 of query aligns to 8:210/217 of 1jwaB

query
sites
1jwaB

M

M

S

L

R

R

Y

Y

A

N

R
|
R

Q

Q

I

I

A

I

V

L

S

R

E

G

F

F

G

D

P

F

H

D

G

G

Q

Q

E

E

A

A

L

L

R

K

H

D

A

S

R

R

L

V

L

L

V

I

I

V

G

G

A

L

G
|
G

L

L

A

G

A

C

P

A

V

A

L

S

P

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

I

L

K

T

L

L

A

L

D
|
D

P
x
F

D

D

I

T

V

V

D

S

L

L

S

S

N

N

L

L

H

Q

R
|
R

Q

Q

T

T

L

L

F

H

T

S

M

D

D

A

D

T

I

V

G

G

L

Q

S

P

K
|
K

A

V

T

E

S

S

A

A

A

R

K

D

H

A

L

L

S

T

A

R

R

I

N

N

P

P

E

H

C

I

L

A

I

I

E

T

T

P

H

V

V

N

A

A

P
x
L

F

L

D

D

P

D

D

A

T

E

A

L

H

A

D

A

L

L

C

I

N

A

D

E

I

H

D

D

L

L

V

V

L

L

D

D

C

C

A

T

D
|
D

S
x
N

F

V

A

A

A
x
V

S

R

Y

N

I

Q

A

L

S

N

D

A

I

G

C

C

G

F

K

A

R

A

N

K

I

V

P

P

L

L

I

V

S

S

A

G

S

A

V

A

V

I

E

R

M

M

G

E

G

G

Y

Q

V

I

G

T

G

V

F

F

C

T

A

Y

G

E

A

A

P

-

S

-

L

-

R

-

A

-

V

-

F

-

P

-

D

-

L

-

P

-

R

-

A

-

S

-

G

-

T

-

C

-

A

-

T

-

L

-

G

G

V

V

L

M

G

A

P

P

I

L

V

I

G

G

I

V

I

I

A

G

T

S

L

L

Q

Q

A

A

Q

M

M

E

A

A

I

I

S

K

V

M

L

L

T

A

G

G

Q

Y

S

G

P

K

S

P

P

A

L

S

G

G

Q

K

L

I

I

V

T

M

F

Y

E

D

G

A

T

M

G

T

F

C

R

Q

F

F

active site: R13 (= R6), D129 (= D122)
binding adenosine-5'-triphosphate: G39 (= G32), G40 (= G33), D61 (= D54), F62 (≠ P55), R72 (= R65), K85 (= K78), L108 (≠ P101), D129 (= D122), N130 (≠ S123), V133 (≠ A126)

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
36% identity, 66% coverage: 1:217/330 of query aligns to 8:232/271 of Q72J02

query
sites
Q72J02

M

L

S

D

R

R

Y

Y

A

H

R

R

Q

Q

I

M

A

I

V

L

S

P

E

Q

F

V

G

G

P

P

H

E

G

G

Q

Q

E

E

A

R

L

L

R

K

H

R

A

A

R

S

L

V

V

V

I

V

G

G

A

A

G

G

L

L

A

G

A

V

P

P

V

V

L

L

P

Q

Y

Y

L

L

A

V

A

A

G

G

V

V

G

G

T

R

I

V

K

G

L

V

A

V

D

E

P

M

D

D

I

R

V

V

D

E

L

V

S

S

N

N

L

L

H

H

R

R

Q

Q

T

V

L

L

F

Y

T

T

M

T

D

E

D

D

I

V

G

G

L

E

S

P

K

K

A

A

T

L

S

V

A

A

A

Q

K

K

H

R

L

L

S

Q

A

A

R

L

N

N

P

P

E

L

C

V

L

R

I

V

E

E

T

A

H

Y

V

P

A

V

P

R

F

L

D

T

P

S

D

E

T

N

A

A

H

L

D

E

L

I

C

L

N

R

D

P

I

Y

D

D

L

L

V

V

L

V

D

D

C

A

A

S

D

D

S

N

F

F

A

P

A

T

S

R

Y

Y

I

L

A

V

S

N

D

D

I

A

C

A

G

V

K

L

R

L

N

G

I

K

P

P

L

L

I

V

S

F

A

G

S

A

V

I

V

Y

E

Q

M

F

G

D

G

G

Y

Q

V

V

G

A

G

V

F

F

-

H

-

P

-

T

-

L

-

H

C

G

A

E

G

M

A

G

P

P

S

C

L

Y

R

R

A

C

V

L

F

F

P

P

D

K

L

P

P

P

R

P

-

P

-

G

A

A

S

V

G

P

T

S

C
|
C

A

A

T

E

L

A

G

G

V

V

L

F

G

G

P

V

I

L

V

P

G

A

I

V

A

G

T

S

L

L

Q

M

A

A

Q

A

M

E

A

A

I

L

S

K

V

V

L

L

T

L

G

G

Q

I

S

G

P

K

S

P

P

L

L

A

G

G

Q

H

L

L

I

L

T

L

F

Y

E

D

C192 (= C177) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

1zud3 Structure of this-thif protein complex (see paper)
36% identity, 67% coverage: 3:223/330 of query aligns to 8:224/240 of 1zud3

query
sites
1zud3

R

R

Y

Y

A

S

R
|
R

Q

Q

I

I

A
x
L

V

L

S
x
D

E

D

F

I

G

A

P

L

H

D

G

G

Q

Q

E

Q

A

K

L

L

R

L

H

D

A

S

R

Q

L

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

A

G

A

T

P

P

V

A

L

A

P

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

L

K

V

L

L

A

A

D

D

P

D

D

D

I

D

V

V

D

H

L

L

S

S

N

N

L

L

H

Q

R

R

Q

Q

T

I

L

L

F

F

T

T

M

T

D

E

D

D

I

I

G

D

L

R

S

P

K

K

A

S

T

Q

S

V

A

S

A

Q

K

Q

H

R

L

L

S

T

A

Q

R

L

N

N

P

P

E

D

C

I

L

Q

I

L

E

T

T

A

H

L

V

Q

A

Q

P

R

F

L

D

T

P

G

D

E

T

A

A

L

H

K

D

D

L

A

C

V

N

A

D

R

I

A

D

D

L

V

V

V

L

L

D

D

C

C

A

T

D
|
D

S

N

F

M

A

A

A

T

S

R

Y

Q

I

E

A

I

S

N

D

A

I

A

C

C

G

V

K

A

R

L

N

N

I

T

P

P

L

L

I

I

S

T

A

A

S

S

V

A

V

V

E

G

M

F

G

G

Y

Q

V

L

G

M

G

V

F

L

C

T

A

P

G

P

A

W

P

E

S

Q

-

G

-
x
C

L

Y

R

R

A

C

V

L

F

W

P

P

D

D

L

N

P

Q

R

E

A

P

S

T

G

A

T

G

C

V

A

V

T

G

L

-

G

-

V

-

L

-

G

-

P

P

I

V

V

V

G

G

I

V

I

M

A

G

T

T

L

L

Q

Q

A

A

Q

L

M

E

A

A

I

I

S

K

V

L

L

L

T

S

G

G

Q

-

S

I

P

E

S

T

P

P

L

A

G

G

Q

E

L

L

I

R

T

L

F

F

E

D

G

G

T

K

G

S

F

S

R

Q

F

W

active site: R11 (= R6), D127 (= D122)
binding calcium ion: L14 (≠ A9), D16 (≠ S11)
binding zinc ion: C169 (vs. gap)

Sites not aligning to the query:

binding zinc ion: 235, 238

Q19360 NEDD8-activating enzyme E1 catalytic subunit; Ectopic membrane ruffles in embryo protein 1; Ubiquitin-activating enzyme 3 homolog; EC 6.2.1.64 from Caenorhabditis elegans (see paper)
37% identity, 37% coverage: 14:134/330 of query aligns to 33:153/430 of Q19360

query
sites
Q19360

G

G

P

P

H

E

G

N

Q

F

E

E

A

A

L

L

R

Q

H

N

A

T

R

K

L

I

L

L

V

V

I

I

G

G

A

A

G

G

L

L

A

G

A

C

P

E

V

L

L

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Y

N

L

L

A

A

A

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A

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G

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G

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T

T

I

I

K

E

L

V

A

I

D

D

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M

D

D

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I

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D

L

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S

N

N

L

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N

R

R

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Q

T

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F

F

T

R

M

E

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A

A

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A

A

K

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A

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R

N

V

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E

G

C

C

L

Q

I

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E

T

T

A

H

H

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N

A

C

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R

F

I

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E

P

-

D

D

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A

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Y

N

R

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I

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A

L

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I

-

P

A

A

A

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R

Y

W

I

I

A

N

S

G

D

M

I

L

C

C

Sites not aligning to the query:

262 A→V: In or198; at 25 degrees Celsius, embryonic lethal due to incomplete cytokinesis with ectopic furrow formation and mis-orientation of mitotic spindle. Reduced cul-3 neddylation.

1y8qB Sumo e1 activating enzyme sae1-sae2-mg-atp complex (see paper)
33% identity, 41% coverage: 19:154/330 of query aligns to 8:141/510 of 1y8qB

query
sites
1y8qB

E

E

A

A

L

V

R

A

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G

A

G

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R

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L

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V

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G

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A

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G

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I

A

G

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V

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L

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Y

N

L

L

A

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A

L

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G

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F

G

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T

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D
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D

P
x
L

D

D

I

T

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I

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D

L

V

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S

N

N

L

L

H

N

R

R

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Q

T

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L

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F

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K

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G

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S

K
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K

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A

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V

A

A

A

K

K

E

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S

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S

L

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F

N

Y

P

P

E

K

C

A

-

N

L

I

I

V

E

A

T

Y

H

H

V

D

A
x
S

P
x
I

F

M

D

N

P

P

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D

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Y

A

N

H

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E

L

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C

F

N

R

D

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I

F

D

I

L

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V

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C

A

A
x
L

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D

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x
N

F

R

A

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R

Y

N

I

H

A

V

S

N

D

R

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M

C

C

G

L

K

A

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A

N

D

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P

L

L

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I

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A

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S

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T

V

-

E

-

M

-

G

A

G

G

Y

Y

V

L

G

G

active site: D112 (= D122)
binding adenosine-5'-triphosphate: D43 (= D54), L44 (≠ P55), K67 (= K78), S90 (≠ A100), I91 (≠ P101), L111 (≠ A121), D112 (= D122), N113 (≠ S123)

Sites not aligning to the query:

3kycB Human sumo e1 complex with a sumo1-amp mimic (see paper)
33% identity, 41% coverage: 19:154/330 of query aligns to 9:142/548 of 3kycB

query
sites
3kycB

E

E

A

A

L

V

R

A

H

G

A

G

R

R

L

V

L

L

V

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I

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A

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G

L

I

A

G

A

C

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V

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L

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Y

N

L

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A

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D

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x
L

D

D

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I

D

D

L

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S

N

N

L

L

H

N

R

R

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Q

T

F

L

L

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K

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G

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K

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A

A

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K

K

E

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L

A

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F

N

Y

P

P

E

K

C

A

-

N

L

I

I

V

E

A

T

Y

H

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x
S

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x
I

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M

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N

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P

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I

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D

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L

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x
L

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D

S
x
N

F

R

A

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S

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Y

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I

H

A

V

S

N

D

R

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M

C

C

G

L

K

A

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A

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D

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P

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L

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I

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E

A

S

S

G

V

T

V

-

E

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M

-

G

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G

G

Y

Y

V

L

G

G

active site: D113 (= D122)
binding 5'-deoxy-5'-(sulfamoylamino)adenosine: G22 (= G32), G23 (= G33), D44 (= D54), L45 (≠ P55), K68 (= K78), S91 (≠ A100), I92 (≠ P101), L112 (≠ A121), D113 (= D122), N114 (≠ S123)

Sites not aligning to the query:

active site: 169, 170, 172
binding zinc ion: 154, 157, 407, 410

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
33% identity, 41% coverage: 19:154/330 of query aligns to 14:147/650 of Q7SXG4

query
sites
Q7SXG4

E

D

A

S

L

L

R

S

H

S

A

C

R

R

L

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L

L

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V

I

V

G

G

A

A

G

G

L

I

A

G

A

C

P

E

V

L

L

L

P

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

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T

N

I

I

K

E

L

V

A

I

D

D

P

L

D

D

I

T

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I

D

D

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V

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S

N

N

L

L

H

N

R

R

Q

Q

T

F

L

L

F

F

T

Q

M

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D

K

D

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V

G

G

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K

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K

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A

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A

A

A

K

K

E

H

S

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S

L

A

R

R

F

N

C

P

P

E

S

C

A

L

N

I

I

E

T

T

A

-

Y

H

H

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D

A

S

P

I

F

M

D

N

P

P

D

D

T

Y

A

N

H

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E

L

F

C

F

N

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D

N

I

F

D

Q

L

L

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V

L

M

D

N

C

A

A

L

D

D

S

N

F

R

A

A

S

R

Y

N

I

H

A

V

S

N

D

R

I

M

C

C

G

L

K

A

R

A

N

D

I

I

P

P

L

L

I

I

S

E

A

S

S

G

V

T

V

-

E

-

M

-

G

A

G

G

Y

Y

V

L

G

G

Sites not aligning to the query:

599 modified: Phosphoserine

3kydB Human sumo e1~sumo1-amp tetrahedral intermediate mimic (see paper)
33% identity, 41% coverage: 19:154/330 of query aligns to 10:143/477 of 3kydB

query
sites
3kydB

E

E

A

A

L

V

R

A

H

G

A

G

R

R

L

V

L

L

V

V

I

V

G

G

A

A

G

G

L
x
I

A

G

A

C

P

E

V

L

L

L

P

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

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S

T

H

I

I

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D

L

L

A

I

D
|
D

P
x
L

D

D

I

T

V

I

D

D

L

V

S

S

N

N

L

L

H

N

R

R

Q

Q

T

F

L

L

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F

T

Q

M

K

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K

D

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G

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K

A

A

T

Q

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V

A

A

A

K

K

E

H

S

L

V

S

L

A

Q

R

F

N

Y

P

P

E

K

C

A

-

N

L

I

I

V

E

A

T

Y

H

H

V

D

A
x
S

P
x
I

F

M

D

N

P

P

D

D

T

Y

A

N

H

V

D

E

L

F

C

F

N

R

D

Q

I

F

D

I

L

L

V

V

L

M

D

N

C

A

A
x
L

D
|
D

S
x
N

F

R

A

A

S

R

Y

N

I

H

A

V

S

N

D

R

I

M

C

C

G

L

K

A

R

A

N

D

I

V

P

P

L

L

I

I

S

E

A

S

S

G

V

T

V

-

E

-

M

-

G

A

G

G

Y

Y

V

L

G

G

active site: D114 (= D122)
binding 5'-{[(3-aminopropyl)sulfonyl]amino}-5'-deoxyadenosine: I25 (≠ L34), D45 (= D54), L46 (≠ P55), K69 (= K78), S92 (≠ A100), I93 (≠ P101), L113 (≠ A121), D114 (= D122), N115 (≠ S123)

Sites not aligning to the query:

active site: 170, 171, 173
binding 5'-{[(3-aminopropyl)sulfonyl]amino}-5'-deoxyadenosine: 170, 173
binding zinc ion: 155, 158, 370, 373

Query Sequence

>WP_090216766.1 NCBI__GCF_002796795.1:WP_090216766.1
MSRYARQIAVSEFGPHGQEALRHARLLVIGAGGLAAPVLPYLAAAGVGTIKLADPDIVDL
SNLHRQTLFTMDDIGLSKATSAAKHLSARNPECLIETHVAPFDPDTAHDLCNDIDLVLDC
ADSFAASYIASDICGKRNIPLISASVVEMGGYVGGFCAGAPSLRAVFPDLPRASGTCATL
GVLGPIVGIIATLQAQMAISVLTGQSPSPLGQLITFEGTGFRFGGFRFDTAPEPEYRPSF
ISAQTIDDTDLIIDLRRPEEGPLAHVTAQRRTVEDIAENPPKADAKTRIVLCCRSGLRAW
AAAEHLSQQHAGPVALVALGDQSDQHGEHS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory