SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_090271795.1 NCBI__GCF_900105005.1:WP_090271795.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
67% identity, 98% coverage: 15:645/647 of query aligns to 14:645/648 of Q89WV5

query
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Q89WV5

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G263 (= G263) mutation to I: Loss of activity.
G266 (= G266) mutation to I: Great decrease in activity.
K269 (= K269) mutation to G: Great decrease in activity.
E414 (= E414) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
61% identity, 99% coverage: 1:642/647 of query aligns to 1:645/652 of P27550

query
sites
P27550

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K609 (= K606) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
60% identity, 99% coverage: 1:642/647 of query aligns to 1:645/652 of Q8ZKF6

query
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Q8ZKF6

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Q

Y

Y

E

E

R

T

M

K

Y

Y

R

K

Q

Q

S

S

I

I

E

N

Q

D

P

P

D

D

M

T

F

F

W

W

A

G

E

E

Q

Q

A

G

N

-

R

K

F

I

V

L

E

D

W

W

M

I

Q

T

P

P

W

Y

S

Q

K

K

V

V

H

K

E

N

S

T

N

S

M

F

E

A

K

P

G

G

E

N

A

V

A

S

-

I

-

K

W

W

F

Y

V

E

D

D

A

G

K

T

L

L

N

N

V

L

S

A

V

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

T

E

R

N

A

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

D

Q

S

S

R

K

Y

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

E

D

V

V

C

C

R

R

L

F

A

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

R

A

D

G

R

R

I

I

Q

I

N

D

A

S

E

S

C

S

D

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

G

A

G

G

K
x
R

T

S

V

I

P

P

L

L

K

K

Q

K

N

N

V

V

D

D

K

D

A

A

L

L

T

K

A

N

C

-

P

P

N

N

V

V

H

T

T

S

V

V

-

E

-

H

-

V

V

I

T

V

V

L

K

K

R

R

T

T

G

G

A

S

D

D

V

I

A

D

W

W

D

Q

E

E

Q

G

K

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

C

I

A

E

Q

K

V

A

S

S

K

P

E

E

C

H

T

Q

P

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

S

Y

V

A

A

A

L

T

T

T

H

F

H

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P

P

T
x
N

Y

W

P

P

T

T

A

P

S

A

R

R

C

M

W

C

E

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

V

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

A

A

L

E

G

G

E

D

E

K

P

A

V

I

K

E

S

G

S

T

S

D

R

R

E

S

S

S

I

L

R

R

L

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

V

A

W

W

E

E

W

W

Y

Y

Y

W

Q

K

V

K

V

I

G

G

D

K

S

E

R

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

S

G

I

F

L

M

I

I

A

T

P

P

L

L

P

P

G

G

A

A

C

I

G

E

L

L

K

K

P

A

G

G

A

S

A

A

M

T

R

R

P

P

M

F

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

E

P

L

Q

D

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

V

V

I

I

K

T

Q

D

S

S

W

W

P

P

S

G

Q

Q

I

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

H

E

R

R

L

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

V

M

Y

Y

F

F

T

S

G

G

D

D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I

I

T

T

G

G

R

R

V

V

D
|
D

D

D

V

V

L

L

N

N

I

V

S
|
S

G
|
G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

L

A

N

H

D

P

Y

K

V

I

A

A

E

E

A

A

V

V

G

G

C

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

A

A

I

I

Y

Y

A

A

Y

Y

V

V

M

T

P

L

M

N

K

H

G

G

V

E

T

E

L

P

T

S

D

P

E

E

V

L

M

Y

A

A

E

E

L

V

K

R

V

N

A

W

V

V

S
x
R

E

K

Q

E

V

I

G

G

A

P

F

L

A

A

R

T

P

P

D

D

F

V

I

L

Q

H

P

W

T

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

C

A

D

G

E

D

L

T

D

S

T

N

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

E

K

L

L

V

L

E

E

H

E

R

K

R194 (≠ K193) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T308) binding CoA
N335 (≠ T332) binding CoA
A357 (= A354) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D514) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S520) binding CoA
G524 (= G521) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R523) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ S581) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K606) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
60% identity, 99% coverage: 5:642/647 of query aligns to 1:638/640 of 5jrhA

query
sites
5jrhA

Q

H

N

K

H

H

K

A

V

I

S

P

E

A

N

N

Q

I

L

A

R

D

Q

R

T

C

H

L

L

I

D

N

Q

P

D

E

G

Q

Y

Y

E

E

R

T

M

K

Y

Y

R

K

Q

Q

S

S

I

I

E

N

Q

D

P

P

D

D

M

T

F

F

W

W

A

G

E

E

Q

Q

A

G

N

-

R

K

F

I

V

L

E

D

W

W

M

I

Q

T

P

P

W

Y

S

Q

K

K

V

V

H

K

E

N

S

T

N

S

M

F

E

A

K

P

G

G

E

N

A

V

A

S

-

I

-

K

W

W

F

Y

V

E

D

D

A

G

K

T

L

L

N

N

V

L

S

A

V

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

T

E

R

N

A

G

D

D

Q

R

T

T

A

A

I

I

W
|
W

E

E

G

G

D

D

P

T

N

S

D

Q

S

S

R

K

Y

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

E

D

V

V

C

C

R

R

L

F

A

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E
|
E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

E

E

A

A

L

V

R

A

D
x
G

R

R

I

I

Q

I

N

D

A

S

E

S

C

S

D

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

G

A

G

G

K

R

T

S

V

I

P

P

L

L

K

K

Q

K

N

N

V

V

D

D

K

D

A

A

L

L

T

K

A

N

C

-

P

P

N

N

V

V

H

T

T

S

V

V

-

E

-

H

-

V

V

I

T

V

V

L

K

K

R

R

T

T

G

G

A

S

D

D

V

I

A

D

W

W

D

Q

E

E

Q

G

K

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

C

I

A

E

Q

K

V

A

S

S

K

P

E

E

C

H

T

Q

P

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

S

Y

V

A

A

A

L

T

T

T

H

F

H

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P

P

T

N

Y

W

P

P

T

T

A

P

S

A

R

R

C

M

W

C

E

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

V

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

A

A

L

E

G

G

E

D

E

K

P

A

V

I

K

E

S

G

S

T

S

D

R

R

E

S

S

S

I

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

V

A

W

W

E

E

W

W

Y

Y

Y

W

Q

K

V

K

V

I

G

G

D

K

S

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

S

G

I

F

L

M

I

I

A

T

P

P

L

L

P

P

G

G

A

A

C

I

G

E

L

L

K

K

P

A

G

G

A

S

A

A

M

T

R

R

P

P

M

F

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

E

P

L

Q

D

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

V

V

I

I

K

T

Q

D

S

S

W

W

P

P

S

G

Q

Q

I

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

H

E

R

R

L

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

V

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

L

L

N
|
N

I

V

S
|
S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

L

A

N
x
H

D

P

Y

K

V
x
I

A

A

E

E

A

A

V

V

G

G

C

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

A

A

I

I

Y

Y

A

A

Y

Y

V

V

M

T

P

L

M

N

K

H

G

G

V

E

T

E

L

P

T

S

D

P

E

E

V

L

M

Y

A

A

E

E

L

V

K

R

V

N

A

W

V

V

S
x
R

E

K

Q

E

V

I

G

G

A
x
P

F

L

A

A

R

T

P

P

D

D

F

V

I

L

Q

H

P

W

T

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

C

A

D

G

E

D

L

-

D

-

T

-

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

E

K

L

L

V

L

E

E

H

E

R

K

active site: T260 (= T261), T412 (= T413), E413 (= E414), N517 (= N518), R522 (= R523), K605 (= K606)
binding (r,r)-2,3-butanediol: W93 (= W96), E140 (= E143), G169 (≠ D172), K266 (= K267), P267 (= P268)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G384), E384 (= E385), P385 (= P386), T408 (= T409), W409 (= W410), W410 (= W411), Q411 (= Q412), T412 (= T413), D496 (= D497), I508 (= I509), N517 (= N518), R522 (= R523)
binding coenzyme a: F159 (= F162), G160 (= G163), G161 (= G164), R187 (= R190), S519 (= S520), R580 (≠ S581), P585 (≠ A586)
binding magnesium ion: V533 (= V534), H535 (≠ N536), I538 (≠ V539)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
60% identity, 99% coverage: 5:642/647 of query aligns to 1:639/641 of 2p20A

query
sites
2p20A

Q

H

N

K

H

H

K

A

V

I

S

P

E

A

N

N

Q

I

L

A

R

D

Q

R

T

C

H

L

L

I

D

N

Q

P

D

E

G

Q

Y

Y

E

E

R

T

M

K

Y

Y

R

K

Q

Q

S

S

I

I

E

N

Q

D

P

P

D

D

M

T

F

F

W

W

A

G

E

E

Q

Q

A

G

N

-

R

K

F

I

V

L

E

D

W

W

M

I

Q

T

P

P

W

Y

S

Q

K

K

V

V

H

K

E

N

S

T

N

S

M

F

E

A

K

P

G

G

E

N

A

V

A

S

-

I

-

K

W

W

F

Y

V

E

D

D

A

G

K

T

L

L

N

N

V

L

S

A

V

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

T

E

R

N

A

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

D

Q

S

S

R

K

Y

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

E

D

V

V

C

C

R

R

L

F

A

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

R

A

D

G

R

R

I

I

Q

I

N

D

A

S

E

S

C

S

D

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

G

A

G

G

K

R

T

S

V

I

P

P

L

L

K

K

Q

K

N

N

V

V

D

D

K

D

A

A

L

L

T

K

A

N

C

-

P

P

N

N

V

V

H

T

T

S

V

V

-

E

-

H

-

V

V

I

T

V

V

L

K

K

R

R

T

T

G

G

A

S

D

D

V

I

A

D

W

W

D

Q

E

E

Q

G

K

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

C

I

A

E

Q

K

V

A

S

S

K

P

E

E

C

H

T

Q

P

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

S

Y

V

A

A

A

L

T

T

T

H

F

H

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P

P

T

N

Y

W

P

P

T

T

A

P

S

A

R

R

C

M

W

C

E

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

V

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

M

M

A

A

L

E

G

G

E

D

E

K

P

A

V

I

K

E

S

G

S

T

S

D

R

R

E

S

S

S

I

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

V

A

W

W

E

E

W

W

Y

Y

Y

W

Q

K

V

K

V

I

G

G

D

K

S

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

S

G

I

F

L

M

I

I

A

T

P

P

L

L

P

P

G

G

A

A

C

I

G

E

L

L

K

K

P

A

G

G

A

S

A

A

M

T

R

R

P

P

M

F

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

E

P

L

Q

D

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

V

V

I

I

K

T

Q

D

S

S

W

W

P

P

S

G

Q

Q

I

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

H

E

R

R

L

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

V

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

I

V

S

S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

L

A

N

H

D

P

Y

K

V

I

A

A

E

E

A

A

V

V

G

G

C

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

A

A

I

I

Y

Y

A

A

Y

Y

V

V

M

T

P

L

M

N

K

H

G

G

V

E

T

E

L

P

T

S

D

P

E

E

V

L

M

Y

A

A

E

E

L

V

K

R

V

N

A

W

V

V

S

A

E

K

Q

E

V

I

G

G

A

P

F

L

A

A

R

T

P

P

D

D

F

V

I

L

Q

H

P

W

T

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

C

A

D

G

E

-

L

-

D

D

T

T

L

S

G

N

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

E

K

L

L

V

L

E

E

H

E

R

K

active site: T260 (= T261), T412 (= T413), E413 (= E414), N517 (= N518), R522 (= R523), K605 (= K606)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G384), E384 (= E385), P385 (= P386), T408 (= T409), W409 (= W410), W410 (= W411), Q411 (= Q412), T412 (= T413), D496 (= D497), I508 (= I509), R511 (= R512), R522 (= R523)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
60% identity, 98% coverage: 6:642/647 of query aligns to 1:635/637 of 2p2fA

query
sites
2p2fA

N

K

H

H

K

A

V

I

S

P

E

A

N

N

Q

I

L

A

R

D

Q

R

T

C

H

L

L

I

D

N

Q

P

D

E

G

Q

Y

Y

E

E

R

T

M

K

Y

Y

R

K

Q

Q

S

S

I

I

E

N

Q

D

P

P

D

D

M

T

F

F

W

W

A

G

E

E

Q

Q

A

G

N

-

R

K

F

I

V

L

E

D

W

W

M

I

Q

T

P

P

W

Y

S

Q

K

K

V

V

H

K

E

N

S

T

N

S

M

F

E

A

K

P

G

G

E

N

A

V

A

S

-

I

-

K

W

W

F

Y

V

E

D

D

A

G

K

T

L

L

N

N

V

L

S

A

V

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

T

E

R

N

A

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

D

Q

S

S

R

K

Y

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

E

D

V

V

C

C

R

R

L

F

A

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

V

V

V

I

F
|
F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

R

A

D

G

R

R

I

I

Q

I

N

D

A

S

E

S

C

S

D

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

G

A

G

G

K

R

T

S

V

I

P

P

L

L

K

K

Q

K

N

N

V

V

D

D

K

D

A

A

L

L

T

K

A

N

C

-

P

P

N

N

V

V

H

T

T

S

V

V

-

E

-

H

-

V

V

I

T

V

V

L

K

K

R

R

T

T

G

G

A

S

D

D

V

I

A

D

W

W

D

Q

E

E

Q

G

K

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

C

I

A

E

Q

K

V

A

S

S

K

P

E

E

C

H

T

Q

P

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

S

Y

V

A

A

A

L

T

T

T

H

F

H

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

V

I

F

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W
|
W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P
|
P

T

N

Y

W

P

P

T

T

A

P

S

A

R

R

C

M

W

C

E

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

V

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

M

M

A

A

L

E

G

G

E

D

E

K

P

A

V

I

K

E

S

G

S

T

S

D

R

R

E

S

S

S

I

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

V

A

W

W

E

E

W

W

Y

Y

Y

W

Q

K

V

K

V

I

G

G

D

K

S

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

S

G

I

F

L

M

I

I

A

T

P

P

L

L

P

P

G

G

A

A

C

I

G

E

L

L

K

K

P

A

G

G

A

S

A

A

M

T

R

R

P

P

M

F

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

E

P

L

Q

D

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

V

V

I

I

K

T

Q

D

S

S

W

W

P

P

S

G

Q

Q

I

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

H

E

R

R

L

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

V

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

I

V

S
|
S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

L

A

N

H

D

P

Y

K

V

I

A

A

E

E

A

A

V

V

G

G

C

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

A

A

I

I

Y

Y

A

A

Y

Y

V

V

M

T

P

L

M

N

K

H

G

G

V

E

T

E

L

P

T

S

D

P

E

E

V

L

M

Y

A

A

E

E

L

V

K

R

V

N

A

W

V

V

S
x
R

E

K

Q

E

V

I

G

G

A
x
P

F

L

A

A

R

T

P

P

D

D

F

V

I

L

Q

H

P

W

T

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

C

A

D

-

E

-

L

-

D

-

T

-

L

-

G

G

D

D

T

T

-

S

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

E

K

L

L

V

L

E

E

H

E

R

K

active site: T259 (= T261), T411 (= T413), E412 (= E414), N516 (= N518), R521 (= R523), K604 (= K606)
binding adenosine monophosphate: G382 (= G384), E383 (= E385), P384 (= P386), T407 (= T409), W408 (= W410), W409 (= W411), Q410 (= Q412), T411 (= T413), D495 (= D497), I507 (= I509), R510 (= R512), N516 (= N518), R521 (= R523)
binding coenzyme a: F158 (= F162), R186 (= R190), W304 (= W306), T306 (= T308), P329 (= P331), A352 (= A354), A355 (= A357), S518 (= S520), R579 (≠ S581), P584 (≠ A586)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
60% identity, 99% coverage: 5:642/647 of query aligns to 1:632/634 of 1pg3A

query
sites
1pg3A

Q

H

N

K

H

H

K

A

V

I

S

P

E

A

N

N

Q

I

L

A

R

D

Q

R

T

C

H

L

L

I

D

N

Q

P

D

E

G

Q

Y

Y

E

E

R

T

M

K

Y

Y

R

K

Q

Q

S

S

I

I

E

N

Q

D

P

P

D

D

M

T

F

F

W

W

A

G

E

E

Q

Q

A

G

N

-

R

K

F

I

V

L

E

D

W

W

M

I

Q

T

P

P

W

Y

S

Q

K

K

V

V

H

K

E

N

S

T

N

S

M

F

E

A

K

P

G

G

E

N

A

V

A

S

-

I

-

K

W

W

F

Y

V

E

D

D

A

G

K

T

L

L

N

N

V

L

S

A

V

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

T

E

R

N

A

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

D

Q

S

S

R

K

Y

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

E

D

V

V

C

C

R

R

L

F

A

A

N

N

V

T

L

L

K

L

E

D

R

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

L

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

F

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

T

S

V

V

V

I

F
|
F

G
|
G

G

G

F

F

S

S

P

P

E

E

A

A

L

V

R

A

D

G

R

C

I

I

Q

I

N

D

A

S

E

S

C

S

D

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

G

A

G

G

K
x
R

T

S

V

I

P

P

L

L

K

K

Q

K

N

N

V

V

D

D

K

D

A

A

L

L

T

K

A

N

C

-

P

P

N

N

V

V

H

T

T

S

V

V

-

E

-

H

-

V

V

I

T

V

V

L

K

K

R

R

T

T

G

G

A

S

D

D

V

I

A

D

W

W

D

Q

E

E

Q

G

K

R

D

D

V

L

W

W

Y

W

H

R

E

D

A

L

C

I

A

E

Q

K

V

A

S

S

K

P

E

E

C

H

T

Q

P

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

S

Y

V

A

A

A

L

T

T

T

H

F

H

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

V

I

F

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W

W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P
|
P

T

N

Y

W

P

P

T

T

A

P

S

A

R

R

C

M

W

C

E

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

V

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

M

M

A

A

L

E

G

G

E

D

E

K

P

A

V

I

K

E

S

G

S

T

S

D

R

R

E

S

S

S

I

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

V

A

W

W

E

E

W

W

Y

Y

Y

W

Q

K

V

K

V

I

G

G

D

K

S

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

S

G

I

F

L

M

I

I

A

T

P

P

L

L

P

P

G

G

A

A

C

I

G

E

L

L

K

K

P

A

G

G

A

S

A

A

M

T

R

R

P

P

M

F

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

E

P

L

Q

D

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

V

V

I

I

K

T

Q

D

S

S

W

W

P

P

S

G

Q

Q

I

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

H

E

R

R

L

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

V

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

D

Y

Y

Y

W

W

I

I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

I

V

S
|
S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

L

A

N
x
H

D

P

Y

K

V
x
I

A

A

E

E

A

A

V

V

G

G

C

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

A

A

I

I

Y

Y

A

A

Y

Y

V

V

M

T

P

L

M

N

K

H

G

G

V

E

T

E

L

P

T

S

D

P

E

E

V

L

M

Y

A

A

E

E

L

V

K

R

V

N

A

W

V

V

S
x
R

E

K

Q

E

V

I

G

G

A
x
P

F

L

A

A

R

T

P

P

D

D

F

V

I

L

Q

H

P

W

T

T

T

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

C

A

D

-

E

-

L

-

D

-

T

-

L

-

G

G

D

D

T

-

S

-

T

-

L

L

A

A

D

D

P

P

G

G

V

V

D

E

E

K

L

L

V

L

E

E

H

E

R

K

active site: T260 (= T261), T412 (= T413), E413 (= E414), N517 (= N518), R522 (= R523), K605 (= K606)
binding coenzyme a: F159 (= F162), G160 (= G163), R187 (= R190), R190 (≠ K193), A301 (= A302), T307 (= T308), P330 (= P331), A356 (= A357), S519 (= S520), R580 (≠ S581), P585 (≠ A586)
binding magnesium ion: V533 (= V534), H535 (≠ N536), I538 (≠ V539)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G384), E384 (= E385), P385 (= P386), T408 (= T409), W409 (= W410), W410 (= W411), Q411 (= Q412), T412 (= T413), D496 (= D497), R511 (= R512), R522 (= R523)

7mmzA Crystal structure of acetyl-coenzyme a synthetase from legionella pneumophila philadelphia 1 in complex with ethyl-amp (see paper)
59% identity, 86% coverage: 38:594/647 of query aligns to 10:557/559 of 7mmzA

query
sites
7mmzA

F

F

W

W

A

S

E

E

Q

I

A

A

N

A

R

Q

F

T

V

I

E

D

W

W

M

I

Q

Q

P

P

W

W

S

S

K

I

V

T

H

L

E

Q

S

G

N

G