SitesBLAST
Comparing WP_090272720.1 NCBI__GCF_900105005.1:WP_090272720.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2d4eC Crystal structure of the hpcc from thermus thermophilus hb8
40% identity, 97% coverage: 9:478/483 of query aligns to 30:500/515 of 2d4eC
- active site: N173 (= N154), K196 (= K177), E271 (= E252), C305 (= C286), E409 (= E385), E486 (= E464)
- binding nicotinamide-adenine-dinucleotide: I169 (= I150), T170 (≠ S151), P171 (= P152), W172 (= W153), K196 (= K177), A198 (= A179), G229 (= G210), G233 (= G214), A234 (≠ D215), T248 (= T229), G249 (= G230), E250 (≠ S231), T253 (= T234), E271 (= E252), L272 (≠ M253), C305 (= C286), E409 (= E385), F411 (= F387), F475 (= F452)
Q56YU0 Aldehyde dehydrogenase family 2 member C4; ALDH1a; Protein REDUCED EPIDERMAL FLUORESCENCE 1; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
38% identity, 97% coverage: 9:478/483 of query aligns to 23:490/501 of Q56YU0
- G152 (≠ V137) mutation to E: In ref1-7; reduced activity on sinapaldehyde.
- G416 (≠ A402) mutation to R: In ref1-6; reduced activity on sinapaldehyde.
6fkuA Structure and function of aldehyde dehydrogenase from thermus thermophilus: an enzyme with an evolutionarily-distinct c-terminal arm (recombinant protein with shortened c-terminal, in complex with NADP) (see paper)
38% identity, 94% coverage: 23:474/483 of query aligns to 28:493/511 of 6fkuA
- active site: N159 (= N154), E261 (= E252), C295 (= C286), E483 (= E464)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I155 (= I150), T156 (≠ S151), N159 (= N154), K182 (= K177), S184 (≠ A179), E185 (= E180), G214 (≠ P209), G215 (= G210), K216 (≠ R211), G220 (= G214), Q221 (≠ D215), F237 (= F228), T238 (= T229), G239 (= G230), S240 (= S231), V243 (≠ T234), E261 (= E252), L262 (≠ M253), C295 (= C286), R342 (≠ S332), F343 (≠ Q333), E404 (= E385), F406 (= F387)
P17202 Aminoaldehyde dehydrogenase BADH; 4-trimethylammoniobutyraldehyde dehydrogenase BADH; Aminobutyraldehyde dehydrogenase BADH; Betaine aldehyde dehydrogenase; SoBADH; EC 1.2.1.-; EC 1.2.1.47; EC 1.2.1.19; EC 1.2.1.8 from Spinacia oleracea (Spinach) (see 3 papers)
35% identity, 97% coverage: 13:481/483 of query aligns to 10:484/497 of P17202
- I28 (vs. gap) binding K(+)
- D96 (≠ E94) binding K(+)
- SPW 156:158 (= SPW 151:153) binding NAD(+)
- Y160 (≠ F155) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-(trimethylamino)butanal.
- W167 (= W162) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- KPSE 182:185 (≠ KPAE 177:180) binding NAD(+)
- L186 (= L181) binding K(+)
- SSAT 236:239 (≠ SEGT 231:234) binding NAD(+)
- V251 (≠ L246) binding in other chain
- L258 (≠ M253) binding NAD(+)
- W285 (≠ Y280) mutation to A: Decreases binding affinity for betaine aldehyde.
- E390 (= E385) binding NAD(+)
- A441 (≠ M436) mutation to I: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- C450 (≠ V446) mutation to S: Loss of partial inactivation by betaine aldehyde in the absence of NAD(+).
- W456 (≠ F452) binding NAD(+); mutation to A: Decreases binding affinity for betaine aldehyde.
- K460 (≠ R456) binding K(+)
7a6qB Crystal structure of human aldehyde dehydrogenase 1a3 in complex with selective nr6 inhibitor compound (see paper)
39% identity, 97% coverage: 9:478/483 of query aligns to 17:484/489 of 7a6qB
- active site: N163 (= N154), E262 (= E252), C296 (= C286), E470 (= E464)
- binding nicotinamide-adenine-dinucleotide: I159 (= I150), W162 (= W153), K186 (= K177), E189 (= E180), G219 (= G210), G223 (= G214), S240 (= S231), V243 (≠ T234), K342 (≠ S332)
- binding (3-oxidanylidene-3-sodiooxy-propanoyl)oxysodium: A32 (≠ E25), T33 (≠ N26), C34 (≠ R27), P36 (= P29), D103 (≠ E94), E189 (= E180), Q190 (≠ L181), F218 (≠ P209), I339 (≠ V329), D340 (= D330)
- binding 3-(2-phenylimidazo[1,2-a]pyridin-6-yl)benzenecarbonitrile: G118 (≠ R108), D141 (≠ G134), N143 (≠ S136), N451 (≠ T443), L453 (≠ V446), A455 (≠ Y448)
7a6qA Crystal structure of human aldehyde dehydrogenase 1a3 in complex with selective nr6 inhibitor compound (see paper)
39% identity, 97% coverage: 9:478/483 of query aligns to 17:484/489 of 7a6qA
- active site: N163 (= N154), E262 (= E252), C296 (= C286), E470 (= E464)
- binding nicotinamide-adenine-dinucleotide: I159 (= I150), T160 (≠ S151), W162 (= W153), K186 (= K177), A188 (= A179), E189 (= E180), G219 (= G210), G223 (= G214), S240 (= S231), V243 (≠ T234), K342 (≠ S332), K346 (≠ Q336)
- binding 3-(2-phenylimidazo[1,2-a]pyridin-6-yl)benzenecarbonitrile: G118 (≠ R108), D141 (≠ G134), N143 (≠ S136), N451 (≠ T443), L453 (≠ V446), Y454 (≠ D447)
5fhzA Human aldehyde dehydrogenase 1a3 complexed with NAD(+) and retinoic acid (see paper)
39% identity, 97% coverage: 9:478/483 of query aligns to 17:484/489 of 5fhzA
- active site: N163 (= N154), K186 (= K177), E262 (= E252), C296 (= C286), E393 (= E385), E470 (= E464)
- binding nicotinamide-adenine-dinucleotide: I159 (= I150), T160 (≠ S151), W162 (= W153), K186 (= K177), E189 (= E180), G219 (= G210), G223 (= G214), F237 (= F228), G239 (= G230), S240 (= S231), T241 (≠ E232), V243 (≠ T234), G264 (= G254), Q343 (= Q333), E393 (= E385)
- binding retinoic acid: G118 (≠ R108), R121 (= R111), F164 (= F155), M168 (≠ I159), W171 (= W162), C295 (≠ R285), C296 (= C286), L453 (≠ V446)
7qk9A Crystal structure of the aldh1a3-atp complex (see paper)
39% identity, 97% coverage: 9:478/483 of query aligns to 16:483/489 of 7qk9A
- binding adenosine-5'-triphosphate: I158 (= I150), T159 (≠ S151), P160 (= P152), W161 (= W153), K185 (= K177), E188 (= E180), G218 (= G210), G222 (= G214), F236 (= F228), S239 (= S231), V242 (≠ T234)
P47895 Retinaldehyde dehydrogenase 3; RALDH-3; RalDH3; Aldehyde dehydrogenase 6; Aldehyde dehydrogenase family 1 member A3; ALDH1A3; EC 1.2.1.36 from Homo sapiens (Human) (see 2 papers)
39% identity, 97% coverage: 9:478/483 of query aligns to 35:502/512 of P47895
- R89 (≠ G62) to C: in MCOP8; does not affect ALDH1A3 expression; results in strongly reduced protein levels; dbSNP:rs397514652
- K204 (= K177) binding NAD(+)
- E207 (= E180) binding NAD(+)
- GSTEVG 257:262 (≠ GSEGTG 230:235) binding NAD(+)
- Q361 (= Q333) binding NAD(+)
- E411 (= E385) binding NAD(+)
- A493 (= A469) to P: in MCOP8; does not affect ALDH1A3 expression; results in strongly reduced protein levels; dbSNP:rs397514653
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
4v37A Crystal structure of betaine aldehyde dehydrogenase from spinach showing a thiohemiacetal with 3-aminopropionaldehyde
35% identity, 97% coverage: 13:481/483 of query aligns to 8:482/495 of 4v37A