SitesBLAST

Comparing WP_090273986.1 NCBI__GCF_900105005.1:WP_090273986.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
39% identity, 96% coverage: 15:412/414 of query aligns to 33:408/412 of 7awmA

• binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ Q74), G89 (≠ H76), G92 (≠ W79), A95 (≠ T82), V96 (= V83), Y99 (≠ F86), M163 (≠ I161), F167 (≠ I165), F293 (= F297), V297 (≠ M301)
• binding aspartic acid: S268 (= S272), S269 (= S273), T306 (= T310), G346 (= G350), I347 (= I351), A350 (= A354), G351 (= G355), D380 (= D384), R383 (= R387), T384 (= T388)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
40% identity, 87% coverage: 43:404/414 of query aligns to 43:412/425 of 6zgbA

• binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S271), S273 (= S272), S274 (= S273), M308 (= M307), D392 (= D384), R395 (= R387), T396 (= T388)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
40% identity, 87% coverage: 43:404/414 of query aligns to 45:414/426 of 6xwnB

• binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S272), S276 (= S273), M310 (= M307), T313 (= T310), D394 (= D384), R397 (= R387)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
40% identity, 87% coverage: 43:404/414 of query aligns to 45:414/427 of 5e9sA

• binding aspartic acid: R274 (≠ S271), S275 (= S272), S276 (= S273), T313 (= T310), G353 (= G350), V354 (≠ I351), A357 (= A354), G358 (= G355), D394 (= D384), R397 (= R387), T398 (= T388)
• binding decyl-beta-d-maltopyranoside: L194 (≠ R190), G198 (≠ W194), Y202 (≠ L198)
• binding sodium ion: Y87 (≠ F86), T90 (= T89), S91 (≠ T90), S276 (= S273), G305 (= G302), A306 (= A303), T307 (= T304), N309 (= N306), N309 (= N306), M310 (= M307), D311 (= D308), S348 (= S345), I349 (≠ S346), G350 (= G347), T351 (≠ A348), N401 (= N391), V402 (= V392), D405 (= D395)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
40% identity, 87% coverage: 43:404/414 of query aligns to 42:411/424 of 6zl4A

• binding decyl-beta-d-maltopyranoside: L191 (≠ R190), G195 (≠ W194), R282 (= R282)
• binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S271), S272 (= S272), S273 (= S273), M307 (= M307), T310 (= T310), G353 (≠ S353), A354 (= A354), R394 (= R387), T395 (= T388)

Sites not aligning to the query:

• binding decyl-beta-d-maltopyranoside: 3

7vr7A Inward-facing structure of human eaat2 in the way213613-bound state (see paper)
39% identity, 89% coverage: 43:409/414 of query aligns to 39:402/402 of 7vr7A

• binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: S57 (= S61), L58 (≠ I62), L65 (= L69), V339 (≠ S346), G340 (= G347), S343 (≠ G350), I344 (= I351)
• binding cholesterol: W188 (= W197), I227 (≠ L234), F250 (≠ W257), W257 (≠ I264), M379 (≠ V386), S382 (≠ A389)
• binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S266 (= S273), M300 (= M307), T303 (= T310), Y306 (= Y313), G348 (= G355), L349 (≠ M356), M352 (= M359), I366 (= I373), L369 (= L376), V370 (≠ L377), D373 (= D380), D377 (= D384), R380 (= R387), T381 (= T388), N384 (= N391)

Sites not aligning to the query:

• binding cholesterol: 2

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
35% identity, 99% coverage: 2:411/414 of query aligns to 11:421/425 of O59010

• S65 (= S61) mutation to V: Strongly decreased chloride conductance.
• R276 (≠ S271) mutation to S: Increased rate of aspartate transport; when associated with R-395.
• RSS 276:278 (≠ SSS 271:273) binding L-aspartate
• M311 (= M307) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
• T314 (= T310) binding L-aspartate
• V355 (≠ I351) binding L-aspartate
• D394 (= D384) binding L-aspartate
• M395 (≠ T385) mutation to R: Increased rate of aspartate transport; when associated with S-276.
• R397 (= R387) mutation to A: Strongly decreased affinity for aspartate.
• N401 (= N391) binding L-aspartate
• D405 (= D395) mutation to N: Strongly decreased affinity for aspartate.

7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
39% identity, 89% coverage: 43:409/414 of query aligns to 47:417/424 of 7xr6A

• binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S272), S281 (= S273), T318 (= T310), G363 (= G355), M367 (= M359), V385 (≠ L377), D388 (= D380), R395 (= R387), T396 (= T388)
• binding dodecyl beta-D-glucopyranoside: W389 (≠ R381)
• binding cholesterol hemisuccinate: R80 (= R77), R84 (≠ T81), I95 (≠ L92), I252 (= I244)

Sites not aligning to the query:

• binding dodecyl beta-D-glucopyranoside: 16, 19, 20

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
40% identity, 87% coverage: 43:404/414 of query aligns to 38:403/416 of 6r7rA

• binding d-aspartic acid: R263 (≠ S271), S265 (= S273), M299 (= M307), T302 (= T310), T340 (≠ A348), G342 (= G350), V343 (≠ I351), G347 (= G355), D383 (= D384), R386 (= R387), T387 (= T388), N390 (= N391)
• binding decyl-beta-d-maltopyranoside: V212 (= V220), A216 (≠ G224)

Sites not aligning to the query:

• binding decyl-beta-d-maltopyranoside: 23

7xr4A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with glutamate (see paper)
38% identity, 89% coverage: 43:409/414 of query aligns to 46:418/425 of 7xr4A

• binding glutamic acid: S282 (= S273), M316 (= M307), T319 (= T310), S359 (≠ G350), G364 (= G355), D393 (= D384), R396 (= R387), T397 (= T388), N400 (= N391)
• binding cholesterol hemisuccinate: R79 (= R77), I253 (= I244)

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
37% identity, 96% coverage: 15:412/414 of query aligns to 25:394/397 of 5mjuA

• binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ V66), S80 (≠ Q74), G81 (≠ H76), G84 (≠ W79), Y91 (≠ F86), M156 (≠ I161), F160 (≠ I165), F286 (= F297), V290 (≠ M301)
• binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (≠ V58), I148 (= I153), S262 (= S273), S263 (= S274), A292 (= A303), T293 (= T304), M296 (= M307), T299 (= T310), G329 (= G347), A336 (= A354), G337 (= G355), D366 (= D384), R369 (= R387), N373 (= N391)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
35% identity, 98% coverage: 2:407/414 of query aligns to 3:409/409 of 6bavA