SitesBLAST
Comparing WP_090274570.1 NCBI__GCF_900105005.1:WP_090274570.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 12 hits to proteins with known functional sites (download)
3dmeA Crystal structure of conserved exported protein from bordetella pertussis. Northeast structural genomics target ber141
51% identity, 99% coverage: 3:367/370 of query aligns to 2:365/366 of 3dmeA
- binding flavin-adenine dinucleotide: I8 (≠ V9), G9 (= G10), G11 (= G12), V12 (= V13), V13 (= V14), E32 (= E33), A33 (≠ R34), T41 (≠ A42), S42 (= S43), R44 (= R45), N45 (= N46), S46 (= S47), V48 (= V49), H50 (= H51), P170 (≠ R170), L171 (≠ V171), A203 (≠ G203), I313 (≠ V315), R314 (= R316), I346 (= I348), E347 (= E349), S348 (= S350), P349 (= P351), G350 (= G352), L351 (= L353), T352 (= T354)
8w78A Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase in complex with fad and 2-oxoglutarate (see paper)
30% identity, 92% coverage: 29:369/370 of query aligns to 30:405/410 of 8w78A
- binding 2-oxoglutaric acid: S48 (= S47), H52 (= H51), Y245 (= Y246), F254 (≠ G255), L255 (= L256), H258 (= H259), R349 (= R316)
- binding flavin-adenine dinucleotide: E34 (= E33), K35 (≠ R34), H42 (= H41), Q43 (≠ A42), S44 (= S43), N47 (= N46), S48 (= S47), V50 (= V49), H52 (= H51), V173 (= V171), G203 (= G203), G204 (= G204), Q206 (≠ F206), R349 (= R316), P387 (= P351), G388 (= G352), A389 (≠ L353), T390 (= T354)
Sites not aligning to the query:
8w7fB Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase bound with fad and a sulfate ion (see paper)
30% identity, 92% coverage: 29:369/370 of query aligns to 30:407/412 of 8w7fB
- binding flavin-adenine dinucleotide: E34 (= E33), K35 (≠ R34), H42 (= H41), Q43 (≠ A42), S44 (= S43), H46 (≠ R45), N47 (= N46), S48 (= S47), V50 (= V49), H52 (= H51), V173 (= V171), G205 (= G203), G206 (= G204), Q208 (≠ F206), Y231 (= Y232), V350 (= V315), R351 (= R316), P389 (= P351), G390 (= G352), A391 (≠ L353), T392 (= T354)
Sites not aligning to the query:
6j39A Crystal structure of cmis2 with inhibitor (see paper)
26% identity, 62% coverage: 14:241/370 of query aligns to 11:237/368 of 6j39A
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: M44 (≠ N46), P46 (≠ E48), N49 (≠ H51)
- binding flavin-adenine dinucleotide: I11 (≠ V14), V29 (≠ I31), D30 (≠ L32), P31 (≠ E33), E32 (≠ R34), K36 (≠ I38), A37 (≠ G39), S38 (≠ E40), V40 (≠ A42), S41 (= S43), A42 (≠ S44), G43 (≠ R45), M44 (≠ N46), A174 (≠ G178), A203 (= A207), W206 (≠ L210), I228 (≠ Y232)
Sites not aligning to the query:
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: 243, 252, 267, 308, 334, 335
- binding flavin-adenine dinucleotide: 7, 9, 10, 252, 308, 333, 334, 335, 336, 337, 338
6j38A Crystal structure of cmis2 (see paper)
26% identity, 62% coverage: 14:241/370 of query aligns to 11:237/368 of 6j38A
- binding flavin-adenine dinucleotide: I11 (≠ V14), V29 (≠ I31), D30 (≠ L32), P31 (≠ E33), K36 (≠ I38), A37 (≠ G39), S38 (≠ E40), S41 (= S43), A42 (≠ S44), G43 (≠ R45), M44 (≠ N46), A174 (≠ G178), A203 (= A207), W206 (≠ L210), G226 (= G230)
Sites not aligning to the query:
Q9HXE3 FAD-dependent catabolic D-arginine dehydrogenase DauA; D-arginine dehydrogenase; DADH; D-arginine utilization protein A; Dau; EC 1.4.99.6 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
25% identity, 60% coverage: 1:223/370 of query aligns to 1:219/375 of Q9HXE3
- A14 (≠ V14) binding FAD
- ER 32:33 (= ER 33:34) binding FAD
- 41:48 (vs. 42:51, 20% identical) binding FAD
- E87 (≠ K89) Important for specificity toward positively charged substrates
- A171 (≠ V171) binding FAD
Sites not aligning to the query:
7rdfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f co-crystallized in the presence of d-arginine (see paper)
25% identity, 60% coverage: 1:223/370 of query aligns to 1:219/375 of 7rdfA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S})-5-[5-[(~{E})-4-carbamimidamidobut-2-enoyl]-7,8-dimethyl-2,4-bis(oxidanylidene)-1~{H}-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: I9 (≠ V9), G10 (= G10), G12 (= G12), I13 (≠ V13), A14 (≠ V14), L31 (= L32), E32 (= E33), R33 (= R34), H40 (= H41), S41 (≠ A42), T42 (≠ S43), R44 (= R45), S45 (≠ N46), A46 (≠ S47), H48 (= H51), Y53 (= Y56), E87 (≠ K89), E170 (≠ R170), A171 (≠ V171), A199 (≠ G203), G200 (= G204), W202 (≠ F206)
- binding 6-hydroxy-flavin-adenine dinucleotide: I9 (≠ V9), G10 (= G10), G12 (= G12), I13 (≠ V13), A14 (≠ V14), E32 (= E33), R33 (= R34), H40 (= H41), S41 (≠ A42), T42 (≠ S43), S45 (≠ N46), A46 (≠ S47), H48 (= H51), E170 (≠ R170), A171 (≠ V171), A199 (≠ G203), G200 (= G204), W202 (≠ F206)
Sites not aligning to the query:
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S})-5-[5-[(~{E})-4-carbamimidamidobut-2-enoyl]-7,8-dimethyl-2,4-bis(oxidanylidene)-1~{H}-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: 222, 249, 303, 305, 330, 331, 332, 333, 334, 335, 336
- binding 6-hydroxy-flavin-adenine dinucleotide: 222, 303, 305, 330, 331, 332, 333, 334, 335, 336
6pldA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with 6-oh-fad - green fraction (see paper)
25% identity, 60% coverage: 1:223/370 of query aligns to 1:219/375 of 6pldA
- binding 6-hydroxy-flavin-adenine dinucleotide: I9 (≠ V9), G10 (= G10), G12 (= G12), I13 (≠ V13), A14 (≠ V14), L31 (= L32), E32 (= E33), R33 (= R34), H40 (= H41), S41 (≠ A42), T42 (≠ S43), R44 (= R45), S45 (≠ N46), A46 (≠ S47), H48 (= H51), E170 (≠ R170), A171 (≠ V171), A199 (≠ G203), G200 (= G204), W202 (≠ F206)
Sites not aligning to the query:
6p9dA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase y249f variant with fad - yellow fraction (see paper)
25% identity, 60% coverage: 1:223/370 of query aligns to 1:219/375 of 6p9dA
- binding dihydroflavine-adenine dinucleotide: I9 (≠ V9), G10 (= G10), G12 (= G12), I13 (≠ V13), A14 (≠ V14), E32 (= E33), R33 (= R34), H40 (= H41), S41 (≠ A42), T42 (≠ S43), R44 (= R45), S45 (≠ N46), A46 (≠ S47), A47 (≠ I50), H48 (= H51), E170 (≠ R170), A171 (≠ V171), A199 (≠ G203), G200 (= G204), W202 (≠ F206)
Sites not aligning to the query:
3sm8A Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with an (n5) flavin adduct (see paper)
25% identity, 60% coverage: 3:223/370 of query aligns to 9:225/381 of 3sm8A
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[7,8-dimethyl-5-(3-methylbutanoyl)-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: I15 (≠ V9), G16 (= G10), G18 (= G12), I19 (≠ V13), A20 (≠ V14), L37 (= L32), E38 (= E33), R39 (= R34), H46 (= H41), S47 (≠ A42), T48 (≠ S43), R50 (= R45), S51 (≠ N46), A52 (≠ S47), H54 (= H51), Y59 (= Y56), E176 (≠ R170), A177 (≠ V171), A205 (≠ G203), G206 (= G204), W208 (≠ F206), I212 (≠ L210)
Sites not aligning to the query:
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[7,8-dimethyl-5-(3-methylbutanoyl)-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: 228, 246, 255, 309, 311, 336, 337, 338, 339, 340, 341, 342
3nyfA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase in complex with imino-histidine (see paper)
25% identity, 60% coverage: 3:223/370 of query aligns to 9:225/381 of 3nyfA
- binding flavin-adenine dinucleotide: I15 (≠ V9), G16 (= G10), G18 (= G12), I19 (≠ V13), A20 (≠ V14), L37 (= L32), E38 (= E33), R39 (= R34), H46 (= H41), S47 (≠ A42), T48 (≠ S43), R50 (= R45), S51 (≠ N46), A52 (≠ S47), H54 (= H51), E176 (≠ R170), A177 (≠ V171), A205 (≠ G203), G206 (= G204), W208 (≠ F206), I212 (≠ L210)
- binding (2Z)-3-(1H-imidazol-5-yl)-2-iminopropanoic acid: H54 (= H51), T56 (≠ G53), Y59 (= Y56), E93 (≠ K89)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 309, 311, 336, 337, 338, 339, 340, 341, 342
- binding (2Z)-3-(1H-imidazol-5-yl)-2-iminopropanoic acid: 228, 246, 255, 311, 338
3nycA Crystal structure of pseudomonas aeruginosa d-arginine dehydrogenase (see paper)
25% identity, 60% coverage: 3:223/370 of query aligns to 9:225/381 of 3nycA
- binding flavin-adenine dinucleotide: I15 (≠ V9), G16 (= G10), G18 (= G12), I19 (≠ V13), A20 (≠ V14), L37 (= L32), E38 (= E33), R39 (= R34), H46 (= H41), S47 (≠ A42), T48 (≠ S43), R50 (= R45), S51 (≠ N46), A53 (≠ I50), H54 (= H51), E176 (≠ R170), A177 (≠ V171), A205 (≠ G203), G206 (= G204), W208 (≠ F206), I212 (≠ L210)
- binding (2E)-5-[(diaminomethylidene)amino]-2-iminopentanoic acid: Y59 (= Y56), E93 (≠ K89)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 309, 311, 336, 337, 338, 339, 340, 341, 342
- binding (2E)-5-[(diaminomethylidene)amino]-2-iminopentanoic acid: 228, 246, 252, 255, 311, 338
Query Sequence
>WP_090274570.1 NCBI__GCF_900105005.1:WP_090274570.1
MADIEVIVVGAGVVGLAIAQRLASGGRDVMILEREASIGEHASSRNSEVIHAGIYYPSGS
LKARLCLDGRDRLYAWCEAHRVPHRRIGKLLVATADDELPALEQLRFNAQACGVQLQSLN
AAQVHELEPAVTAVGGLFSPLTGIVDSHALMQSFETVLLGQGGQLCLRTRVDRVERQGER
WLVQGQSSGQPFAVSCRWLINAGGLFAQQLAHSIDCYPAALIPPLHYCQGRYFSYSGSSP
FRHLVYPMPEPNAVGLGVHATLDLGGQLRFGPDVRYTDSIDYQVDAGSGESFARSIQRYW
PECRAERLQPGYAGVRAKLSGPGAAAADFMIQGPAIHGLQGVVSLFGIESPGLTASLALA
EEVAGQLDNL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory