SitesBLAST
Comparing WP_090274851.1 NCBI__GCF_900105005.1:WP_090274851.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A6W2K8 CoA-transferase/lyase DddD; EC 2.-.-.- from Marinomonas sp. (strain MWYL1) (see paper)
24% identity, 81% coverage: 6:607/746 of query aligns to 9:685/837 of A6W2K8
- D602 (= D539) mutation to A: Abolishes ability to produce dimethyl sulfide (DMS).
9br7C Succinate--hydroxymethylglutarate CoA-transferase (see paper)
28% identity, 49% coverage: 379:744/746 of query aligns to 1:397/403 of 9br7C
5yx6A Crystal structure of rv3272 from m. Tuberculosis orthorhombic form (see paper)
28% identity, 46% coverage: 379:721/746 of query aligns to 1:356/360 of 5yx6A
Q5U921 (R)-2-hydroxy-4-methylpentanoate CoA-transferase; 2-hydroxyisocaproate-CoA transferase; EC 2.8.3.24 from Clostridioides difficile (Peptoclostridium difficile) (see paper)
26% identity, 49% coverage: 384:745/746 of query aligns to 3:398/399 of Q5U921
- D171 (= D539) mutation D->A,N: Loss of activity.
2gd6A The 1,1-proton transfer reaction mechanism by alpha-methylacyl-coa racemase is catalyzed by an aspartate/histidine pair and involves a smooth, methionine-rich surface for binding the fatty acyl moiety (see paper)
30% identity, 37% coverage: 383:657/746 of query aligns to 3:277/354 of 2gd6A
- active site: G16 (≠ L396), D121 (= D510), D150 (= D539), G213 (≠ T600), G214 (= G601)
- binding acetyl coenzyme *a: I15 (≠ Y395), R37 (≠ A417), A53 (≠ G432), D54 (≠ T433), L55 (≠ Q434), K56 (≠ R435), G77 (≠ N466), Y78 (≠ M467), R79 (= R468), V82 (≠ A471), R85 (≠ K474), G119 (= G508), H120 (≠ F509), Y124 (≠ Q514), D150 (= D539), M182 (≠ L570)
2gd2A The 1,1-proton transfer reaction mechanism by alpha-methylacyl-coa racemase is catalyzed by an aspartate/histidine pair and involves a smooth, methionine-rich surface for binding the fatty acyl moiety (see paper)
30% identity, 37% coverage: 383:657/746 of query aligns to 3:277/354 of 2gd2A
- active site: G16 (≠ L396), D121 (= D510), D150 (= D539), G213 (≠ T600), G214 (= G601)
- binding acetoacetyl-coenzyme a: I15 (≠ Y395), R37 (≠ A417), A53 (≠ G432), L55 (≠ Q434), K56 (≠ R435), G77 (≠ N466), Y78 (≠ M467), R79 (= R468), V82 (≠ A471), R85 (≠ K474), L86 (= L475), A118 (≠ P507), G119 (= G508), H120 (≠ F509), Y124 (≠ Q514), D150 (= D539)
2gd0A The 1,1-proton transfer reaction mechanism by alpha-methylacyl-coa racemase is catalyzed by an aspartate/histidine pair and involves a smooth, methionine-rich surface for binding the fatty acyl moiety (see paper)
30% identity, 37% coverage: 383:657/746 of query aligns to 3:277/354 of 2gd0A
- active site: G16 (≠ L396), D121 (= D510), D150 (= D539), G213 (≠ T600), G214 (= G601)
- binding (s)-2-methylmyristoyl-coenzyme a: D42 (= D421), L55 (≠ Q434), K56 (≠ R435), G77 (≠ N466), Y78 (≠ M467), R79 (= R468), V82 (≠ A471), R85 (≠ K474), L86 (= L475), G119 (= G508), H120 (≠ F509), D121 (= D510), Y124 (≠ Q514), D150 (= D539)
2gciA The 1,1-proton transfer reaction mechanism by alpha-methylacyl-coa racemase is catalyzed by an asparte/histidine pair and involves a smooth, methionine-rich surface for binding the fatty acyl moiety (see paper)
30% identity, 37% coverage: 383:657/746 of query aligns to 3:277/354 of 2gciA
- active site: G16 (≠ L396), D121 (= D510), D150 (= D539), G213 (≠ T600), G214 (= G601)
- binding (r)-2-methylmyristoyl-coenzyme a: R37 (≠ A417), L55 (≠ Q434), K56 (≠ R435), G77 (≠ N466), Y78 (≠ M467), R79 (= R468), V82 (≠ A471), G119 (= G508), H120 (≠ F509), D121 (= D510), Y124 (≠ Q514), D150 (= D539), Y218 (≠ H606), I234 (≠ L621), E235 (≠ A622)
2gceA The 1,1-proton transfer reaction mechanism by alpha-methylacyl-coa racemase is catalyzed by an aspartate/histidine pair and involves a smooth, methionine-rich surface for binding the fatty acyl moiety (see paper)
30% identity, 37% coverage: 383:657/746 of query aligns to 3:277/354 of 2gceA
- active site: G16 (≠ L396), D121 (= D510), D150 (= D539), G213 (≠ T600), G214 (= G601)
- binding (r)-ibuprofenoyl-coenzyme a: I15 (≠ Y395), R37 (≠ A417), L55 (≠ Q434), K56 (≠ R435), G77 (≠ N466), Y78 (≠ M467), R79 (= R468), V82 (≠ A471), R85 (≠ K474), G119 (= G508), H120 (≠ F509), D121 (= D510), Y124 (≠ Q514), D150 (= D539), L211 (≠ T598), Y218 (≠ H606), I234 (≠ L621)
- binding (s)-ibuprofenoyl-coenzyme a: I15 (≠ Y395), G16 (≠ L396), P17 (≠ A397), R37 (≠ A417), L55 (≠ Q434), K56 (≠ R435), G77 (≠ N466), Y78 (≠ M467), R79 (= R468), V82 (≠ A471), R85 (≠ K474), G119 (= G508), H120 (≠ F509), Y124 (≠ Q514), D150 (= D539)
2yimA The enolisation chemistry of a thioester-dependent racemase: the 1.4 a crystal structure of a complex with a planar reaction intermediate analogue (see paper)
30% identity, 37% coverage: 383:657/746 of query aligns to 3:278/355 of 2yimA
- active site: G16 (≠ L396), D122 (= D510), D151 (= D539), G214 (≠ T600), G215 (= G601)
- binding 2-methylacetoacetyl coa: I15 (≠ Y395), R37 (≠ A417), A54 (≠ G432), L56 (≠ Q434), K57 (≠ R435), G78 (≠ N466), Y79 (≠ M467), R80 (= R468), V83 (≠ A471), R86 (≠ K474), L87 (= L475), A119 (≠ P507), G120 (= G508), H121 (≠ F509), Y125 (≠ Q514), D151 (= D539)
3ubmB Formyl-coa:oxalate coa-transferase from acetobacter aceti (see paper)
24% identity, 48% coverage: 383:738/746 of query aligns to 4:422/430 of 3ubmB
- active site: Q17 (≠ L396), E140 (≠ D510), D182 (= D539), G261 (vs. gap), G262 (vs. gap)
- binding coenzyme a: V16 (≠ Y395), R38 (vs. gap), L72 (= L442), N73 (≠ K443), T74 (≠ L444), K75 (≠ G445), N96 (= N466), F97 (≠ M467), R98 (= R468), A101 (= A471), R104 (≠ K474), K125 (≠ S495), D182 (= D539), M213 (≠ L570)
O06543 Alpha-methylacyl-CoA racemase; AMACR; MtMCR; EC 5.1.99.4 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
30% identity, 37% coverage: 383:657/746 of query aligns to 4:283/360 of O06543
- R38 (≠ A417) binding substrate
- R52 (= R425) mutation to A: 15.7% of wild-type activity.
- I56 (= I429) mutation to P: 28.8% of wild-type activity.
- ADLK 59:62 (≠ GTQR 432:435) binding substrate
- E82 (≠ H465) mutation to A: 12.5% of wild-type activity.
- GYR 83:85 (≠ NMR 466:468) binding substrate
- R91 (≠ K474) binding substrate; mutation to A: 19.9% of wild-type activity.
- M111 (≠ V494) mutation to P: 5.2% of wild-type activity.
- GHD-INY 125:130 (≠ GFDQLMQ 508:514) binding substrate
- H126 (≠ F509) mutation to A: 4.5% of wild-type activity.
- D156 (= D539) mutation to A: 17.6 of wild-type activity.
- D190 (vs. gap) mutation to A: 3.3% of wild-type activity.
- E241 (≠ A622) mutation to A: 2.1% of wild-type activity.
Sites not aligning to the query:
- 297 C→A: 6.2% of wild-type activity.
- 312 H→A: 10.1% of wild-type activity.
Q9UHK6 Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase; EC 5.1.99.4 from Homo sapiens (Human) (see 5 papers)
24% identity, 48% coverage: 384:744/746 of query aligns to 3:373/382 of Q9UHK6