SitesBLAST
Comparing WP_090446271.1 NCBI__GCF_900100495.1:WP_090446271.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
44% identity, 95% coverage: 7:255/261 of query aligns to 3:244/244 of 6t77A
- active site: G16 (= G22), S138 (= S152), Y151 (= Y166)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G16), S14 (≠ D20), R15 (≠ S21), T37 (≠ S43), L58 (≠ A66), N59 (≠ D67), V60 (≠ L68), A87 (= A95), G88 (= G96), I89 (≠ M97)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
43% identity, 94% coverage: 10:255/261 of query aligns to 9:243/243 of 4i08A
- active site: G19 (= G22), N113 (= N124), S141 (= S152), Q151 (≠ E163), Y154 (= Y166), K158 (= K170)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G16), S17 (≠ D20), R18 (≠ S21), I20 (= I23), T40 (≠ S43), N62 (≠ D67), V63 (≠ L68), N89 (= N94), A90 (= A95), G140 (≠ S151), S141 (= S152), Y154 (= Y166), K158 (= K170), P184 (= P196), G185 (= G197), T189 (= T201)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
43% identity, 94% coverage: 10:255/261 of query aligns to 9:247/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G16), S17 (≠ D20), R18 (≠ S21), I20 (= I23), T40 (≠ S43), N62 (≠ D67), V63 (≠ L68), N89 (= N94), A90 (= A95), I92 (≠ M97), V139 (≠ I150), S141 (= S152), Y154 (= Y166), K158 (= K170), P184 (= P196), G185 (= G197), I187 (= I199), T189 (= T201), M191 (≠ S203)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
43% identity, 95% coverage: 7:255/261 of query aligns to 3:244/244 of P0A2C9
- M125 (= M139) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A234) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S235) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 95% coverage: 7:255/261 of query aligns to 3:244/244 of P0AEK2
- GA--SR 12:15 (≠ GAGSDS 16:21) binding NADP(+)
- T37 (≠ S43) binding NADP(+)
- NV 59:60 (≠ DL 67:68) binding NADP(+)
- N86 (= N94) binding NADP(+)
- Y151 (= Y166) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YSAAK 166:170) binding NADP(+)
- A154 (= A169) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K170) mutation to A: Defect in the affinity for NADPH.
- I184 (= I199) binding NADP(+)
- E233 (= E244) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
42% identity, 95% coverage: 7:255/261 of query aligns to 2:243/243 of 1q7bA
- active site: G15 (= G22), E101 (≠ T116), S137 (= S152), Q147 (≠ E163), Y150 (= Y166), K154 (= K170)
- binding calcium ion: E232 (= E244), T233 (≠ V245)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G16), S13 (≠ D20), R14 (≠ S21), T36 (≠ S43), N58 (≠ D67), V59 (≠ L68), N85 (= N94), A86 (= A95), G87 (= G96), I88 (≠ M97), S137 (= S152), Y150 (= Y166), K154 (= K170), P180 (= P196), G181 (= G197), I183 (= I199)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
42% identity, 95% coverage: 7:255/261 of query aligns to 2:243/243 of 1q7cA
- active site: G15 (= G22), S137 (= S152), Q147 (≠ E163), F150 (≠ Y166), K154 (= K170)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G16), S13 (≠ D20), R14 (≠ S21), A35 (= A42), T36 (≠ S43), L57 (≠ A66), N58 (≠ D67), V59 (≠ L68), G87 (= G96), I88 (≠ M97)
4k6fB X-ray crystal structure of a putative acetoacetyl-coa reductase from burkholderia cenocepacia bound to the co-factor NADP
39% identity, 93% coverage: 9:251/261 of query aligns to 1:241/245 of 4k6fB
- active site: G12 (= G22), N102 (≠ T116), S138 (= S152), Y151 (= Y166), K155 (= K170)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G16), Y32 (≠ A42), S33 (= S43), N36 (≠ R46), V58 (≠ A66), D59 (= D67), V60 (≠ L68), A87 (= A95), G88 (= G96), I89 (≠ M97)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
39% identity, 93% coverage: 8:251/261 of query aligns to 6:241/244 of 4nbuB
- active site: G18 (= G22), N111 (= N124), S139 (= S152), Q149 (≠ E163), Y152 (= Y166), K156 (= K170)
- binding acetoacetyl-coenzyme a: D93 (≠ Q100), K98 (≠ G111), S139 (= S152), N146 (= N160), V147 (≠ P161), Q149 (≠ E163), Y152 (= Y166), F184 (≠ W198), M189 (≠ S203), K200 (≠ Y214)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G16), N17 (≠ S19), G18 (= G22), I19 (= I23), D38 (≠ A42), F39 (≠ S43), V59 (≠ A63), D60 (= D67), V61 (≠ L68), N87 (= N94), A88 (= A95), G89 (= G96), I90 (≠ M97), T137 (≠ I150), S139 (= S152), Y152 (= Y166), K156 (= K170), P182 (= P196), F184 (≠ W198), T185 (≠ I199), T187 (= T201), M189 (≠ S203)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
40% identity, 95% coverage: 7:255/261 of query aligns to 2:243/243 of 7emgB
5vt6A Crystal structure of acetoacetyl-coa reductase from burkholderia pseudomallei 1710b complexed with NADP
38% identity, 93% coverage: 9:251/261 of query aligns to 1:241/245 of 5vt6A
- active site: G12 (= G22), D102 (≠ T116), S138 (= S152), Y151 (= Y166), K155 (= K170)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G16), G11 (≠ S19), G12 (= G22), L13 (≠ I23), H32 (≠ A42), S33 (= S43), N36 (≠ I47), V58 (≠ A66), D59 (= D67), V60 (≠ L68), N86 (= N94), A87 (= A95), G88 (= G96), I89 (≠ M97), I136 (= I150), Y151 (= Y166), K155 (= K170), P181 (= P196), Y183 (≠ W198), L184 (≠ I199), T186 (= T201)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
41% identity, 94% coverage: 10:255/261 of query aligns to 7:247/247 of P73574
- A14 (= A17) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P161) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K170) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ W198) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ H210) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
38% identity, 92% coverage: 12:251/261 of query aligns to 4:236/239 of 3sj7A
- active site: G12 (= G22), S138 (= S152), Q148 (≠ E163), Y151 (= Y166), K155 (= K170)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G16), S10 (≠ G18), R11 (≠ S19), I13 (= I23), N31 (≠ T41), Y32 (vs. gap), A33 (vs. gap), G34 (≠ A42), S35 (= S43), A58 (= A66), N59 (≠ D67), V60 (≠ L68), N86 (= N94), A87 (= A95), T109 (≠ R123), S138 (= S152), Y151 (= Y166), K155 (= K170), P181 (= P196), G182 (= G197)
4bo4C Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with n-(2-methoxyphenyl)-3,4- dihydro-2h-quinoline-1-carboxamide at 2.7a resolution (see paper)
42% identity, 93% coverage: 10:251/261 of query aligns to 19:251/255 of 4bo4C
3tzcA Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg)(y155f) from vibrio cholerae (see paper)
41% identity, 93% coverage: 10:251/261 of query aligns to 9:222/224 of 3tzcA
P16544 Putative ketoacyl reductase; EC 1.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
38% identity, 93% coverage: 10:251/261 of query aligns to 7:257/261 of P16544
- 11:39 (vs. 14:44, 61% identical) binding NADP(+)
- D63 (= D67) binding NADP(+)
- K161 (= K170) binding NADP(+)
2rh4A Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
38% identity, 93% coverage: 10:251/261 of query aligns to 3:253/257 of 2rh4A
- active site: G13 (= G22), N110 (= N124), S140 (= S152), Y153 (= Y166), K157 (= K170), Y198 (≠ A211)
- binding 3-methyl-1,6,8-trihydroxyanthraquinone: T141 (= T153), Q145 (≠ R157), V147 (≠ S159), Y153 (= Y166), F185 (≠ W198)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G16), T11 (≠ D20), S12 (= S21), G13 (= G22), I14 (= I23), A33 (= A42), R34 (≠ S43), G35 (≠ S44), C58 (≠ A66), D59 (= D67), V60 (≠ L68), N86 (= N94), G88 (= G96), S140 (= S152), Y153 (= Y166), K157 (= K170), P183 (= P196), G184 (= G197), V186 (≠ I199), T188 (= T201), M190 (≠ S203)
Sites not aligning to the query:
1xr3A Actinorhodin polyketide ketoreductase with NADP and the inhibitor isoniazid bound (see paper)
38% identity, 93% coverage: 10:251/261 of query aligns to 2:252/256 of 1xr3A
- active site: G12 (= G22), N109 (= N124), S139 (= S152), Y152 (= Y166), K156 (= K170), Y197 (≠ A211)
- binding 4-(diazenylcarbonyl)pyridine: T140 (= T153), G141 (≠ T154), V146 (≠ S159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G16), T10 (≠ D20), S11 (= S21), G12 (= G22), I13 (= I23), A32 (= A42), R33 (≠ S43), G34 (≠ S44), C57 (≠ A66), D58 (= D67), V59 (≠ L68), N85 (= N94), A86 (= A95), G87 (= G96), S139 (= S152), Y152 (= Y166), K156 (= K170), G183 (= G197), V185 (≠ I199), T187 (= T201), P188 (≠ G202)
1w4zA Structure of actinorhodin polyketide (actiii) reductase (see paper)
38% identity, 93% coverage: 10:251/261 of query aligns to 5:255/259 of 1w4zA
- active site: G15 (= G22), N112 (= N124), S142 (= S152), Y155 (= Y166), K159 (= K170), Y200 (≠ A211)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G16), T13 (≠ D20), S14 (= S21), G15 (= G22), I16 (= I23), R36 (≠ S43), G37 (≠ S44), D61 (= D67), V62 (≠ L68), N88 (= N94), G90 (= G96), S142 (= S152), Y155 (= Y166), K159 (= K170), P185 (= P196), G186 (= G197), V188 (≠ I199), T190 (= T201)
2rh4B Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
38% identity, 93% coverage: 10:251/261 of query aligns to 14:264/268 of 2rh4B
- active site: G24 (= G22), N121 (= N124), S151 (= S152), Y164 (= Y166), K168 (= K170), Y209 (≠ A211)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G16), T22 (≠ D20), S23 (= S21), I25 (= I23), A44 (= A42), R45 (≠ S43), G46 (≠ S44), C69 (≠ A66), D70 (= D67), V71 (≠ L68), N97 (= N94), S151 (= S152), Y164 (= Y166), K168 (= K170), G195 (= G197), V197 (≠ I199), T199 (= T201), M201 (≠ S203)
Query Sequence
>WP_090446271.1 NCBI__GCF_900100495.1:WP_090446271.1
MKTHSIWREQVALVSGAGSDSGIGLAIARRLGREGARVLLTASSARIEQRARELQAEGID
ARALVADLTDETQVAELASWAQAQFGRVDILVNNAGMAMQGSPEPFAEVAGMDLATWNLS
IARNLTSAFLLTRALLPGMQARGYGRIVNISSTTGTRGSNPGEAAYSAAKAGMLGLSMGL
ALEVARQGITVNSVAPGWIATGSSTEEERHAAEYAPIGRAGRPEEVAAAVAFLASPEASY
ITGEVLVVDGGNCLIENKAPQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory