SitesBLAST
Comparing WP_090447225.1 NCBI__GCF_900100495.1:WP_090447225.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
31% identity, 96% coverage: 8:261/265 of query aligns to 4:254/259 of 5zaiC
- active site: A65 (≠ G69), F70 (≠ M74), S82 (≠ R86), R86 (= R90), G110 (= G117), E113 (≠ G120), P132 (= P139), E133 (= E140), I138 (= I145), P140 (= P147), G141 (≠ A148), A226 (≠ D233), F236 (≠ V243)
- binding coenzyme a: K24 (≠ S28), L25 (≠ R29), A63 (= A67), G64 (= G68), A65 (≠ G69), D66 (= D70), I67 (= I71), P132 (= P139), R166 (= R172), F248 (= F255), K251 (= K258)
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
31% identity, 93% coverage: 18:263/265 of query aligns to 21:265/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
34% identity, 97% coverage: 8:265/265 of query aligns to 3:255/256 of 3h81A
- active site: A64 (≠ G69), M69 (= M74), T79 (≠ Y85), F83 (≠ N89), G107 (= G117), E110 (≠ G120), P129 (= P139), E130 (= E140), V135 (≠ I145), P137 (= P147), G138 (≠ A148), L223 (≠ D233), F233 (≠ V243)
- binding calcium ion: F233 (≠ V243), Q238 (≠ G248)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
34% identity, 97% coverage: 8:263/265 of query aligns to 4:254/255 of 3q0jC
- active site: A65 (≠ G69), M70 (= M74), T80 (≠ Y85), F84 (≠ N89), G108 (= G117), E111 (≠ G120), P130 (= P139), E131 (= E140), V136 (≠ I145), P138 (= P147), G139 (≠ A148), L224 (≠ D233), F234 (≠ V243)
- binding acetoacetyl-coenzyme a: Q23 (≠ D27), A24 (≠ S28), L25 (≠ R29), A27 (= A31), A63 (= A67), G64 (= G68), A65 (≠ G69), D66 (= D70), I67 (= I71), K68 (= K72), M70 (= M74), F84 (≠ N89), G107 (= G116), G108 (= G117), E111 (≠ G120), P130 (= P139), E131 (= E140), P138 (= P147), G139 (≠ A148), M140 (≠ Q149)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
34% identity, 97% coverage: 8:263/265 of query aligns to 4:254/255 of 3q0gC
- active site: A65 (≠ G69), M70 (= M74), T80 (≠ Y85), F84 (≠ N89), G108 (= G117), E111 (≠ G120), P130 (= P139), E131 (= E140), V136 (≠ I145), P138 (= P147), G139 (≠ A148), L224 (≠ D233), F234 (≠ V243)
- binding coenzyme a: L25 (≠ R29), A63 (= A67), I67 (= I71), K68 (= K72), Y104 (≠ A113), P130 (= P139), E131 (= E140), L134 (= L143)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
34% identity, 97% coverage: 8:263/265 of query aligns to 3:249/250 of 3q0gD
- active site: A64 (≠ G69), M69 (= M74), T75 (≠ Y85), F79 (≠ N89), G103 (= G117), E106 (≠ G120), P125 (= P139), E126 (= E140), V131 (≠ I145), P133 (= P147), G134 (≠ A148), L219 (≠ D233), F229 (≠ V243)
- binding Butyryl Coenzyme A: F225 (= F239), F241 (= F255)
6l3pA Crystal strcuture of feruloyl-coa hydratase lyase(fchl) complexed with coa
34% identity, 67% coverage: 9:185/265 of query aligns to 9:183/244 of 6l3pA
- active site: M69 (≠ G69), Y74 (≠ M74), R86 (= R86), Q90 (≠ R90), G114 (= G117), S117 (≠ G120), S136 (≠ P139), E137 (= E140), I142 (= I145), P144 (= P147), G145 (≠ A148)
- binding coenzyme a: K28 (≠ S28), R29 (= R29), A31 (= A31), A67 (= A67), M69 (≠ G69), D70 (= D70), L71 (≠ I71), G113 (= G116)
Sites not aligning to the query:
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
33% identity, 98% coverage: 5:263/265 of query aligns to 2:258/261 of 5jbxB
- active site: A67 (≠ G69), R72 (≠ M74), L84 (≠ R86), R88 (≠ N89), G112 (= G117), E115 (≠ G120), T134 (≠ P139), E135 (= E140), I140 (= I145), P142 (= P147), G143 (≠ A148), A228 (≠ G237), L238 (≠ V243)
- binding coenzyme a: S24 (≠ D27), R25 (≠ S28), R26 (= R29), A28 (= A31), A65 (= A67), D68 (= D70), L69 (≠ I71), K70 (= K72), L110 (= L115), G111 (= G116), T134 (≠ P139), E135 (= E140), L138 (= L143), R168 (= R172)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
31% identity, 93% coverage: 18:263/265 of query aligns to 18:263/266 of O53561
- K135 (≠ Q135) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 135:142, 38% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ S142) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
32% identity, 93% coverage: 17:263/265 of query aligns to 12:254/257 of 6slbAAA
- active site: Q64 (≠ G69), F69 (≠ M74), L80 (≠ Y85), N84 (= N89), A108 (≠ G117), S111 (≠ G120), A130 (≠ P139), F131 (≠ E140), L136 (≠ I145), P138 (= P147), D139 (≠ A148), A224 (≠ D233), G234 (≠ V243)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ G63), A62 (= A67), Q64 (≠ G69), D65 (= D70), L66 (≠ I71), Y76 (≠ G81), A108 (≠ G117), F131 (≠ E140), D139 (≠ A148)
4izdA Crystal structure of dmdd e121a in complex with mmpa-coa (see paper)
33% identity, 87% coverage: 15:245/265 of query aligns to 17:238/253 of 4izdA
- active site: L70 (≠ G69), H75 (≠ M74), C89 (vs. gap), H93 (≠ N89), G117 (= G117), A120 (≠ G120), E140 (= E140), G148 (≠ A148), Y232 (≠ F239)
- binding 3-methylmercaptopropionate-CoA (MMPA-CoA): D29 (= D27), K30 (≠ S28), R31 (= R29), A33 (= A31), A68 (= A67), L70 (≠ G69), D71 (= D70), L72 (≠ I71)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
31% identity, 70% coverage: 21:206/265 of query aligns to 18:202/362 of 3bptA
- active site: G67 (= G69), P84 (vs. gap), R88 (= R86), G115 (= G117), G118 (= G120), E138 (= E140), D146 (≠ A148)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G68), G67 (= G69), I69 (= I71), E90 (vs. gap), G114 (= G116), G115 (= G117), E138 (= E140), D146 (≠ A148), V147 (≠ Q149)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ S28), L26 (≠ R29), A28 (= A31), G66 (= G68), G67 (= G69), I69 (= I71), P137 (= P139), I141 (≠ L143)
Sites not aligning to the query:
4izcB Crystal structure of dmdd e121a in complex with mta-coa (see paper)
33% identity, 87% coverage: 15:245/265 of query aligns to 17:238/266 of 4izcB
- active site: L70 (≠ G69), H75 (≠ M74), C89 (≠ N89), H93 (≠ G93), G117 (= G117), A120 (≠ G120), E140 (= E140), G148 (≠ A148), Y232 (≠ F239)
- binding methylthioacryloyl-CoA: D29 (= D27), K30 (≠ S28), R31 (= R29), A33 (= A31), A68 (= A67), L70 (≠ G69), D71 (= D70), L72 (≠ I71), W92 (≠ F92), G117 (= G117), P139 (= P139), E140 (= E140), R143 (≠ L143), G148 (≠ A148)
Q5HH38 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Staphylococcus aureus (strain COL) (see paper)
29% identity, 99% coverage: 4:265/265 of query aligns to 9:268/273 of Q5HH38
- R34 (= R29) binding in other chain
- SGGDQ 73:77 (≠ AGGDI 67:71) binding in other chain
- S149 (≠ I145) binding in other chain
Q5LLW6 Methylthioacryloyl-CoA hydratase; EC 4.2.1.155 from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) (see paper)
33% identity, 87% coverage: 15:245/265 of query aligns to 18:239/267 of Q5LLW6
- K31 (≠ S28) binding 3-(methylsulfanyl)acryloyl-CoA
- R32 (= R29) binding 3-(methylsulfanyl)acryloyl-CoA
- A69 (= A67) binding 3-(methylsulfanyl)acryloyl-CoA
- L71 (≠ G69) binding 3-(methylsulfanyl)acryloyl-CoA
- L73 (≠ I71) binding 3-(methylsulfanyl)acryloyl-CoA
- G118 (= G117) binding 3-(methylsulfanyl)acryloyl-CoA
- E121 (≠ G120) active site, Nucleophile; mutation to A: Abolishes catalytic activity.
- E141 (= E140) active site, Proton acceptor; mutation to A: Abolishes catalytic activity.
- R144 (≠ L143) binding 3-(methylsulfanyl)acryloyl-CoA
- G149 (≠ A148) binding 3-(methylsulfanyl)acryloyl-CoA
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 68% coverage: 10:190/265 of query aligns to 11:193/378 of Q9LKJ1
- G70 (= G69) mutation to S: Loss of activity.
- E142 (= E140) mutation to A: Loss of activity.
- D150 (≠ A148) mutation to G: Reduced activity.
Q62651 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; EC 5.3.3.- from Rattus norvegicus (Rat) (see paper)
28% identity, 89% coverage: 1:236/265 of query aligns to 49:294/327 of Q62651
- D176 (≠ G120) mutation D->A,D: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- E196 (= E140) mutation E->D,Q: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- D204 (vs. gap) mutation D->A,N: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
32% identity, 93% coverage: 17:263/265 of query aligns to 9:242/245 of 6slaAAA
- active site: Q61 (≠ G69), L68 (≠ Y85), N72 (= N89), A96 (≠ G117), S99 (≠ G120), A118 (≠ P139), F119 (≠ E140), L124 (≠ I145), P126 (= P147), N127 (≠ A148), A212 (≠ D233), G222 (≠ V243)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ R29), A59 (= A67), Q61 (≠ G69), D62 (= D70), L63 (≠ I71), L68 (≠ Y85), Y71 (≠ L88), A94 (≠ L115), G95 (= G116), A96 (≠ G117), F119 (≠ E140), I122 (≠ L143), L124 (≠ I145), N127 (≠ A148), F234 (= F255), K237 (= K258)
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 95% coverage: 13:265/265 of query aligns to 82:332/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
4i52A Scmenb im complex with 1-hydroxy-2-naphthoyl-coa (see paper)
27% identity, 98% coverage: 6:265/265 of query aligns to 8:270/275 of 4i52A
- active site: G77 (= G69), R82 (vs. gap), Y87 (vs. gap), R95 (= R78), L99 (≠ R86), G123 (= G117), V126 (≠ G120), G146 (≠ E140), S151 (≠ I145), D153 (≠ P147), G154 (≠ A148), A240 (= A235), Y248 (≠ V243)
- binding 1-hydroxy-2-naphthoyl-CoA: H29 (≠ D27), K30 (≠ S28), R31 (= R29), A33 (= A31), S75 (≠ A67), G76 (= G68), G77 (= G69), D78 (= D70), Q79 (vs. gap), L96 (≠ A79), V98 (≠ Y85), Y119 (≠ A113), I121 (≠ L115), G123 (= G117), T145 (≠ P139), V149 (≠ L143), S151 (≠ I145), F152 (≠ L146)
Query Sequence
>WP_090447225.1 NCBI__GCF_900100495.1:WP_090447225.1
MSDLPHCETLLLSLEAGVLHITLNRPDSRNAMSLAMVEELRAVLAAVNDELDVRAIVLRG
TGGHFCAGGDIKDMATARAQGEQAYRELNRAFGSLLEEAQRAPQVLVAVLEGAVLGGGFG
LACVSDIAIARQEAQFGLPETSLGILPAQIAPFVVGRIGLTQARRLALTAARFNGAEAAR
LGLVHFCEASSEALERRLEETLAQIRQCAPQANAATKALLLATETENLGSLLDHAAGQFA
EAVLGAEGSEGTLAFVQKRAPKWAQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory