SitesBLAST
Comparing WP_090447360.1 NCBI__GCF_900100495.1:WP_090447360.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6ie0B X-ray crystal structure of 2r,3r-butanediol dehydrogenase from bacillus subtilis
42% identity, 99% coverage: 1:351/356 of query aligns to 2:346/347 of 6ie0B
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
35% identity, 92% coverage: 23:350/356 of query aligns to 24:344/347 of 5vm2A
- active site: C39 (= C38), G40 (= G39), S41 (= S40), H44 (= H43), H65 (= H71), E66 (= E72), C95 (= C101), C98 (= C104), C101 (= C107), C109 (= C115), D113 (≠ A119), P153 (= P157), G157 (= G161), K340 (= K346)
- binding magnesium ion: H65 (= H71), E66 (= E72), E152 (= E156)
- binding zinc ion: C95 (= C101), C98 (= C104), C101 (= C107), C109 (= C115)
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
30% identity, 98% coverage: 3:350/356 of query aligns to 4:345/348 of 1e3jA
- active site: C38 (= C38), G39 (= G39), S40 (= S40), H43 (= H43), H63 (= H71), E64 (= E72), C93 (= C101), C96 (= C104), C99 (= C107), C107 (= C115), T111 (≠ A119), P150 (= P157), G154 (= G161), K341 (= K346)
- binding phosphate ion: A174 (= A181), A196 (= A208), R197 (= R209), S198 (≠ K210), R201 (≠ A213)
- binding zinc ion: C38 (= C38), H63 (= H71), E64 (= E72), C93 (= C101), C96 (= C104), C99 (= C107), C107 (= C115)
3qe3A Sheep liver sorbitol dehydrogenase (see paper)
33% identity, 92% coverage: 5:331/356 of query aligns to 7:327/351 of 3qe3A
- active site: C39 (= C38), G40 (= G39), S41 (= S40), H44 (= H43), H64 (= H71), E65 (= E72), R94 (≠ C101), D97 (≠ C104), C100 (= C107), S108 (≠ C115), F112 (≠ A119), P151 (= P157), G155 (= G161)
- binding glycerol: Y45 (≠ E44), F54 (= F51), T116 (≠ L123), R293 (≠ A297)
- binding zinc ion: C39 (= C38), H64 (= H71), E65 (= E72)
Sites not aligning to the query:
4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
34% identity, 92% coverage: 1:328/356 of query aligns to 4:322/342 of 4ejmA
- active site: C40 (= C38), G41 (= G39), T42 (≠ S40), H45 (= H43), H61 (= H71), E62 (= E72), C91 (= C101), C94 (= C104), C97 (= C107), C105 (= C115), R109 (≠ A119), P147 (= P157), C151 (≠ G161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G180), G172 (= G182), V173 (≠ T183), I174 (= I184), T194 (≠ E204), R195 (≠ M205), Q196 (≠ S206), K199 (≠ R209), C240 (= C248), E245 (≠ S253), T246 (= T254), L263 (≠ V271), V265 (≠ I273), I291 (≠ A297)
- binding zinc ion: C91 (= C101), C94 (= C104), C97 (= C107), C105 (= C115)
Sites not aligning to the query:
4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
34% identity, 92% coverage: 1:328/356 of query aligns to 4:322/343 of 4ej6A
- active site: C40 (= C38), G41 (= G39), T42 (≠ S40), H45 (= H43), H61 (= H71), E62 (= E72), C91 (= C101), C94 (= C104), C97 (= C107), C105 (= C115), R109 (≠ A119), P147 (= P157), C151 (≠ G161)
- binding zinc ion: C91 (= C101), C94 (= C104), C97 (= C107), C105 (= C115)
Sites not aligning to the query:
Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
32% identity, 92% coverage: 5:331/356 of query aligns to 13:333/357 of Q00796
- C45 (= C38) binding Zn(2+)
- H70 (= H71) binding Zn(2+)
- E71 (= E72) binding Zn(2+)
- R110 (≠ Q111) to P: in HMNR8; results in protein aggregation
- H135 (≠ V135) to R: in HMNR8; results in protein aggregation
- A153 (= A153) to D: in HMNR8; uncertain significance; results in protein aggregation; dbSNP:rs145813597
- I184 (= I184) binding NAD(+)
- D204 (≠ E204) binding NAD(+)
- R209 (= R209) binding NAD(+)
- Q239 (≠ G239) to L: in dbSNP:rs1042079
- N269 (≠ R267) to T: in dbSNP:rs930337
- VGL 273:275 (≠ VGI 271:273) binding NAD(+)
- VFR 297:299 (≠ SLA 295:297) binding NAD(+)
- V322 (≠ I320) to I: in HMNR8; uncertain significance; dbSNP:rs149975952
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
33% identity, 92% coverage: 5:331/356 of query aligns to 11:330/354 of P07846
- C43 (= C38) binding Zn(2+)
- Y49 (= Y45) binding substrate
- H67 (= H71) binding Zn(2+)
- E68 (= E72) binding Zn(2+)
- E153 (= E156) binding substrate
- R296 (≠ A297) binding substrate
- Y297 (= Y298) binding substrate
1pl6A Human sdh/nadh/inhibitor complex (see paper)
32% identity, 92% coverage: 5:331/356 of query aligns to 12:332/356 of 1pl6A
- active site: C44 (= C38), G45 (= G39), S46 (= S40), H49 (= H43), H69 (= H71), E70 (= E72), R99 (≠ C101), D102 (≠ C104), C105 (= C107), S113 (≠ C115), F117 (≠ A119), P156 (= P157), G160 (= G161)
- binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C38), S46 (= S40), I56 (≠ V50), F59 (= F51), H69 (= H71), E155 (= E156), L274 (≠ I273), F297 (≠ L296)
- binding nicotinamide-adenine-dinucleotide: G181 (= G182), P182 (≠ T183), I183 (= I184), D203 (≠ E204), L204 (≠ M205), R208 (= R209), C249 (= C248), T250 (≠ I249), V272 (= V271), G273 (= G272), L274 (≠ I273), F297 (≠ L296), R298 (≠ A297)
- binding zinc ion: C44 (= C38), H69 (= H71)
Sites not aligning to the query:
2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
33% identity, 94% coverage: 13:345/356 of query aligns to 16:341/346 of 2dfvA
- active site: C40 (= C38), G41 (= G39), T42 (≠ S40), H45 (= H43), H65 (= H71), E66 (= E72), C95 (= C101), C98 (= C104), C101 (= C107), C109 (= C115), K113 (≠ A119), P151 (= P157), A155 (≠ G161), K340 (≠ N344)
- binding nicotinamide-adenine-dinucleotide: G175 (= G182), P176 (≠ T183), L177 (≠ I184), E197 (= E204), P198 (≠ M205), R202 (= R209), F241 (≠ C248), S242 (≠ I249), A244 (≠ H251), L264 (≠ V271), G265 (= G272), L266 (≠ I273), I289 (≠ L296), T290 (≠ A297)
- binding zinc ion: C95 (= C101), C101 (= C107), C109 (= C115)
O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
33% identity, 94% coverage: 13:345/356 of query aligns to 18:343/348 of O58389
- C42 (= C38) binding Zn(2+)
- T44 (≠ S40) mutation to A: Total loss of enzymatic activity.
- H67 (= H71) binding Zn(2+)
- E68 (= E72) binding Zn(2+)
- C97 (= C101) binding Zn(2+)
- C100 (= C104) binding Zn(2+)
- C103 (= C107) binding Zn(2+)
- C111 (= C115) binding Zn(2+)
- E152 (= E156) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
- L179 (≠ I184) binding NAD(+)
- E199 (= E204) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- R204 (= R209) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- LGL 266:268 (≠ VGI 271:273) binding NAD(+)
- IT 291:292 (≠ LA 296:297) binding NAD(+)
- R294 (vs. gap) mutation to A: 4000-fold decrease in catalytic efficiency.
3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
33% identity, 91% coverage: 13:335/356 of query aligns to 16:331/347 of 3gfbA
- active site: C40 (= C38), G41 (= G39), T42 (≠ S40), H45 (= H43), H65 (= H71), E66 (= E72), C95 (= C101), C98 (= C104), C101 (= C107), C109 (= C115), K113 (≠ A119), P151 (= P157), A155 (≠ G161)
- binding nicotinamide-adenine-dinucleotide: G173 (= G180), G175 (= G182), P176 (≠ T183), L177 (≠ I184), S196 (≠ L203), E197 (= E204), P198 (≠ M205), R202 (= R209), F241 (≠ C248), S242 (≠ I249), A244 (≠ H251), L264 (≠ V271), G265 (= G272), L266 (≠ I273), I289 (= I293), T290 (≠ G294)
Sites not aligning to the query:
Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
33% identity, 91% coverage: 13:335/356 of query aligns to 18:333/350 of Q5JI69
- L179 (≠ I184) binding NAD(+)
- E199 (= E204) binding NAD(+)
- R204 (= R209) binding NAD(+)
- LGL 266:268 (≠ VGI 271:273) binding NAD(+)
- IT 291:292 (≠ IG 293:294) binding NAD(+)
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
32% identity, 92% coverage: 5:331/356 of query aligns to 13:333/357 of P27867
- C45 (= C38) binding Zn(2+)
- H70 (= H71) binding Zn(2+)
- E71 (= E72) binding Zn(2+)
- E156 (= E156) binding Zn(2+)
- D204 (≠ E204) binding NAD(+)
- R209 (= R209) binding NAD(+)
- VGM 273:275 (≠ VGI 271:273) binding NAD(+)
- VFR 297:299 (≠ SLA 295:297) binding NAD(+)
4uejA Closed state of galactitol-1-phosphate 5-dehydrogenase from e. Coli in complex with glycerol. (see paper)
31% identity, 92% coverage: 12:340/356 of query aligns to 12:336/346 of 4uejA
- active site: C38 (= C38), G39 (= G39), S40 (= S40), P43 (≠ H43), H59 (= H71), E60 (= E72), C89 (= C101), C92 (= C104), C95 (= C107), C103 (= C115), D107 (≠ A119), P145 (= P157), G149 (= G161)
- binding glycerol: H59 (= H71), E144 (= E156)
- binding zinc ion: C89 (= C101), C92 (= C104), C95 (= C107), C103 (= C115)
Sites not aligning to the query:
4a2cA Crystal structure of galactitol-1-phosphate dehydrogenase from escherichia coli (see paper)
31% identity, 92% coverage: 12:340/356 of query aligns to 12:336/346 of 4a2cA
- active site: C38 (= C38), G39 (= G39), S40 (= S40), P43 (≠ H43), H59 (= H71), E60 (= E72), C89 (= C101), C92 (= C104), C95 (= C107), C103 (= C115), D107 (≠ A119), P145 (= P157), G149 (= G161)
- binding zinc ion: C89 (= C101), C92 (= C104), C95 (= C107), C103 (= C115)
Sites not aligning to the query:
4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
31% identity, 86% coverage: 23:329/356 of query aligns to 25:316/337 of 4ilkA
- active site: C40 (= C38), G41 (= G39), S42 (= S40), H45 (= H43), H59 (= H71), E60 (= E72), C89 (= C101), C92 (= C104), C95 (= C107), C103 (= C115), A107 (= A119), P145 (= P157), A149 (≠ G161)
- binding manganese (ii) ion: C40 (= C38), H59 (= H71), E60 (= E72), E144 (= E156)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G180), G170 (= G182), P171 (≠ T183), I172 (= I184), D193 (≠ E204), R194 (≠ M205), R198 (= R209), N213 (≠ P224), A235 (≠ C248), A236 (≠ I249), C237 (≠ G250), I241 (≠ T254), M258 (≠ V271), F260 (≠ I273)
- binding zinc ion: C89 (= C101), C92 (= C104), C95 (= C107), C103 (= C115)
Sites not aligning to the query:
7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
30% identity, 92% coverage: 5:331/356 of query aligns to 7:338/357 of 7y9pA
2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
31% identity, 94% coverage: 19:353/356 of query aligns to 20:343/343 of 2ejvA
- active site: C38 (= C38), G39 (= G39), T40 (≠ S40), H43 (= H43), H63 (= H71), E64 (= E72), C93 (= C101), C96 (= C104), C99 (= C107), C107 (= C115), Q111 (≠ A119), P149 (= P157), A153 (≠ G161), K336 (= K346)
- binding nicotinamide-adenine-dinucleotide: G172 (= G180), G174 (= G182), P175 (≠ T183), I176 (= I184), S195 (≠ L203), D196 (≠ E204), P197 (≠ M205), R201 (= R209), F238 (≠ C248), S239 (≠ I249), N241 (≠ H251), A244 (≠ T254), L261 (≠ V271), G262 (= G272), I263 (= I273)
- binding zinc ion: C38 (= C38), H63 (= H71), E64 (= E72), C96 (= C104), C99 (= C107), C107 (= C115)
2dq4A Crystal structure of threonine 3-dehydrogenase
31% identity, 94% coverage: 19:353/356 of query aligns to 20:343/343 of 2dq4A
- active site: C38 (= C38), G39 (= G39), T40 (≠ S40), H43 (= H43), H63 (= H71), E64 (= E72), C93 (= C101), C96 (= C104), C99 (= C107), C107 (= C115), Q111 (≠ A119), P149 (= P157), A153 (≠ G161), K336 (= K346)
- binding zinc ion: C38 (= C38), H63 (= H71), E64 (= E72), C93 (= C101), C96 (= C104), C107 (= C115)
Query Sequence
>WP_090447360.1 NCBI__GCF_900100495.1:WP_090447360.1
MTAAVWHGRKDIRLERVAVPEPPQPGWAQIKVHWCGICGSDLHEYLAGPVFIPMDRPHPL
TGLQGQCILGHEFCGEIVALGAGVSDFAVGQRVAADACQHCGQCLFCRQGQYNLCENLAF
TGLMNNGAFAELVNVPANLLYRLPEGFPSEAGALIEPLAVGMHAVNKAGSLHEQTVVVVG
AGTIGLCTIMCAKAAGAARIIALEMSAARKAKALEVGASEVIDPSQCDAVAAVRELTDGY
GAAVSFECIGHKSTAKLAIEVIRKAGRCVMVGIFEEPSEFNFFEIVATEKQVIGSLAYAG
EFAEVIALIAAGRLDVAPLITGRIGLQQIIEQGFEELVNNKDHNVKIIVSPAALAS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory