SitesBLAST
Comparing WP_090448278.1 NCBI__GCF_900100495.1:WP_090448278.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 6 hits to proteins with known functional sites (download)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
26% identity, 87% coverage: 29:398/427 of query aligns to 26:382/383 of 5i39A
- active site: F66 (≠ N69), Q69 (≠ N74), A70 (= A75), Q248 (≠ D265), P267 (≠ D280)
- binding flavin-adenine dinucleotide: V30 (≠ I33), G31 (= G34), G33 (= G36), I34 (= I37), L35 (≠ T38), V53 (≠ L56), E54 (= E57), K55 (≠ A58), Q62 (≠ G65), S63 (= S66), F66 (≠ N69), Y67 (≠ V70), Q69 (≠ N74), A196 (≠ P206), A197 (≠ V207), G226 (≠ S235), G227 (= G241), W229 (= W243), Q248 (≠ D265), Q250 (= Q267), G321 (= G338), M323 (≠ I340), T348 (≠ N364), G349 (= G365), W350 (≠ R366), G351 (= G367), M352 (≠ V368), T353 (= T369)
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
26% identity, 84% coverage: 28:387/427 of query aligns to 26:388/824 of Q8GAI3
- W66 (≠ G67) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (≠ R68) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 85% coverage: 27:388/427 of query aligns to 1:349/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (= I33), G8 (= G34), G10 (= G36), V11 (≠ I37), I12 (≠ T38), V30 (≠ L56), E31 (= E57), K32 (≠ A58), E38 (≠ G64), A39 (≠ G65), S40 (= S66), A43 (≠ N69), G45 (= G71), L46 (= L72), V171 (= V207), G200 (≠ S235), G201 (= G241), W203 (= W243), G298 (= G338), R300 (≠ A341), P301 (≠ F342), Y326 (≠ N364), R327 (≠ G365), N328 (≠ R366), G329 (= G367), I330 (≠ V368)
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
26% identity, 51% coverage: 29:246/427 of query aligns to 18:224/433 of 5hxwA
- active site: F58 (≠ N69), Q61 (≠ N74), A62 (= A75)
- binding flavin-adenine dinucleotide: V22 (≠ I33), G23 (= G34), G25 (= G36), I26 (= I37), L27 (≠ T38), E46 (= E57), K47 (≠ A58), E53 (≠ G64), Q54 (≠ G65), S55 (= S66), R57 (= R68), F58 (≠ N69), Y59 (≠ V70), G60 (= G71), Q61 (≠ N74), A188 (≠ P206), A189 (≠ V207), G218 (≠ S235), G219 (= G241), W221 (= W243)
Sites not aligning to the query:
- active site: 240, 284, 288
- binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 240, 242, 331, 371, 373, 398, 399, 400, 401, 402, 403
3dmeA Crystal structure of conserved exported protein from bordetella pertussis. Northeast structural genomics target ber141
29% identity, 42% coverage: 28:207/427 of query aligns to 3:171/366 of 3dmeA
- binding flavin-adenine dinucleotide: I8 (= I33), G9 (= G34), G11 (= G36), V12 (≠ I37), V13 (≠ T38), E32 (= E57), A33 (= A58), T41 (≠ G65), S42 (= S66), R44 (= R68), N45 (= N69), S46 (≠ V70), V48 (≠ L72), H50 (≠ N74), P170 (= P206), L171 (≠ V207)
Sites not aligning to the query:
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
26% identity, 53% coverage: 27:252/427 of query aligns to 4:221/369 of S5FMM4
- G51 (≠ N74) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- A54 (≠ V84) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- K81 (≠ I111) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
- S202 (= S235) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
Sites not aligning to the query:
- 332 I→V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- 342 M→V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Query Sequence
>WP_090448278.1 NCBI__GCF_900100495.1:WP_090448278.1
MSLRQECLWEKLTPQRPTAAALGGELRVDVCVIGAGITGLSAALHLLEQGKTVCVLEAHE
TGHGGSGRNVGLVNAGMWIAPDEVEAKLGAAVGGKLLASMGAAPALVFGLIDKYAIDCQA
RREGTLHMAHNARGQADLQSRCAQWQRRGAPVELLSGAACQQATGTQRIAAALLDRRAGT
LNPMAYTSGLARAVTGLGGQLFHHSPVQRLERQGASWCVVTAGGAVSAEQVVLASNAYTE
GEWSELRRNFFPGYYYQVASAPLEDEQAARILPGGQGAWDTRQVLSSIRRDADGRLLLGS
LGNGAAKPTWFLRAWAERIQRHYFPQLGRVNWQSTWTGCIAFTPDHLMRLFEPAPGLLAV
TGYNGRGVTTGSVVGKAFADYLCQGDPQLLPMPLQPLRRVAAARLRSGLYEAGFSLYHAA
QCLRVVV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory