SitesBLAST
Comparing WP_090560791.1 NCBI__GCF_900101615.1:WP_090560791.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6dkhC The crystal structure of l-idonate 5-dehydrogenase from escherichia coli str. K-12 substr. Mg1655
43% identity, 100% coverage: 2:343/343 of query aligns to 9:346/346 of 6dkhC
1pl6A Human sdh/nadh/inhibitor complex (see paper)
36% identity, 95% coverage: 3:328/343 of query aligns to 10:335/356 of 1pl6A
- active site: C44 (= C37), G45 (= G38), S46 (= S39), H49 (= H42), H69 (= H62), E70 (= E63), R99 (≠ C92), D102 (≠ C95), C105 (= C98), S113 (≠ C106), F117 (≠ R110), P156 (= P153), G160 (≠ C157)
- binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C37), S46 (= S39), I56 (≠ F49), F59 (≠ V52), H69 (= H62), E155 (= E152), L274 (= L268), F297 (= F290)
- binding nicotinamide-adenine-dinucleotide: G181 (= G178), P182 (= P179), I183 (= I180), D203 (= D200), L204 (= L201), R208 (≠ P205), C249 (≠ A243), T250 (≠ S244), V272 (≠ L266), G273 (= G267), L274 (= L268), F297 (= F290), R298 (= R291)
- binding zinc ion: C44 (= C37), H69 (= H62)
Sites not aligning to the query:
Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
36% identity, 95% coverage: 3:328/343 of query aligns to 11:336/357 of Q00796
- C45 (= C37) binding Zn(2+)
- H70 (= H62) binding Zn(2+)
- E71 (= E63) binding Zn(2+)
- R110 (= R102) to P: in HMNR8; results in protein aggregation
- H135 (≠ C131) to R: in HMNR8; results in protein aggregation
- A153 (= A149) to D: in HMNR8; uncertain significance; results in protein aggregation; dbSNP:rs145813597
- I184 (= I180) binding NAD(+)
- D204 (= D200) binding NAD(+)
- R209 (≠ P205) binding NAD(+)
- Q239 (≠ Y236) to L: in dbSNP:rs1042079
- N269 (≠ V262) to T: in dbSNP:rs930337
- VGL 273:275 (≠ LGL 266:268) binding NAD(+)
- VFR 297:299 (≠ TFR 289:291) binding NAD(+)
- V322 (≠ L314) to I: in HMNR8; uncertain significance; dbSNP:rs149975952
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3qe3A Sheep liver sorbitol dehydrogenase (see paper)
34% identity, 99% coverage: 3:341/343 of query aligns to 5:342/351 of 3qe3A
- active site: C39 (= C37), G40 (= G38), S41 (= S39), H44 (= H42), H64 (= H62), E65 (= E63), R94 (≠ C92), D97 (≠ C95), C100 (= C98), S108 (≠ C106), F112 (≠ R110), P151 (= P153), G155 (≠ C157), K339 (= K338)
- binding glycerol: Y45 (= Y43), F54 (≠ V52), T116 (≠ S114), R293 (= R291)
- binding zinc ion: C39 (= C37), H64 (= H62), E65 (= E63)
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
33% identity, 99% coverage: 3:341/343 of query aligns to 11:348/357 of P27867
- C45 (= C37) binding Zn(2+)
- H70 (= H62) binding Zn(2+)
- E71 (= E63) binding Zn(2+)
- E156 (= E152) binding Zn(2+)
- D204 (= D200) binding NAD(+)
- R209 (≠ P205) binding NAD(+)
- VGM 273:275 (≠ LGL 266:268) binding NAD(+)
- VFR 297:299 (≠ TFR 289:291) binding NAD(+)
P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
33% identity, 99% coverage: 3:341/343 of query aligns to 9:345/354 of P07846
- C43 (= C37) binding Zn(2+)
- Y49 (= Y43) binding substrate
- H67 (= H62) binding Zn(2+)
- E68 (= E63) binding Zn(2+)
- E153 (= E152) binding substrate
- R296 (= R291) binding substrate
- Y297 (≠ F292) binding substrate
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
33% identity, 99% coverage: 3:342/343 of query aligns to 4:345/348 of 1e3jA
- active site: C38 (= C37), G39 (= G38), S40 (= S39), H43 (= H42), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C99 (= C98), C107 (= C106), T111 (≠ R110), P150 (= P153), G154 (≠ C157), K341 (= K338)
- binding phosphate ion: A174 (= A177), A196 (≠ D200), R197 (≠ L201), S198 (≠ H202), R201 (≠ P205)
- binding zinc ion: C38 (= C37), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C99 (= C98), C107 (= C106)
4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
33% identity, 100% coverage: 1:343/343 of query aligns to 4:337/337 of 4ilkA
- active site: C40 (= C37), G41 (= G38), S42 (= S39), H45 (= H42), H59 (= H62), E60 (= E63), C89 (= C92), C92 (= C95), C95 (= C98), C103 (= C106), A107 (≠ R110), P145 (= P153), A149 (≠ C157), K332 (= K338)
- binding manganese (ii) ion: C40 (= C37), H59 (= H62), E60 (= E63), E144 (= E152)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G176), G170 (= G178), P171 (= P179), I172 (= I180), D193 (= D200), R194 (≠ L201), R198 (≠ P205), N213 (≠ M220), A235 (= A243), A236 (≠ S244), C237 (≠ G245), I241 (≠ A249), M258 (≠ L266), F260 (≠ L268)
- binding zinc ion: C89 (= C92), C92 (= C95), C95 (= C98), C103 (= C106)
7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
33% identity, 95% coverage: 3:329/343 of query aligns to 5:342/357 of 7y9pA
A2QAC0 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) (see 2 papers)
34% identity, 92% coverage: 25:341/343 of query aligns to 43:370/386 of A2QAC0
- M70 (≠ V52) mutation to F: Abolishes enzyme activity.
- DI 213:214 (≠ DL 200:201) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-359.
- Y318 (≠ F290) mutation to F: Increases affinity for D-sorbitol.
- A359 (= A331) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 213-SR-214.
B6HI95 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum) (see paper)
33% identity, 93% coverage: 24:341/343 of query aligns to 41:369/385 of B6HI95
- DI 212:213 (≠ DL 200:201) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-358.
- S358 (≠ A331) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 212-SR-213.
4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
31% identity, 94% coverage: 1:323/343 of query aligns to 4:323/343 of 4ej6A
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H61 (= H62), E62 (= E63), C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106), R109 (= R110), P147 (= P153), C151 (= C157)
- binding zinc ion: C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106)
Sites not aligning to the query:
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
31% identity, 94% coverage: 23:343/343 of query aligns to 24:340/341 of P07913
- C38 (= C37) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
31% identity, 94% coverage: 1:323/343 of query aligns to 4:323/342 of 4ejmA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H61 (= H62), E62 (= E63), C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106), R109 (= R110), P147 (= P153), C151 (= C157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G176), G172 (= G178), V173 (≠ P179), I174 (= I180), T194 (≠ D200), R195 (≠ L201), Q196 (≠ H202), K199 (≠ P205), C240 (≠ A243), E245 (≠ A248), T246 (≠ A249), L263 (= L266), V265 (≠ L268), I291 (≠ R291)
- binding zinc ion: C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106)
Sites not aligning to the query:
3m6iA L-arabinitol 4-dehydrogenase (see paper)
32% identity, 99% coverage: 4:341/343 of query aligns to 8:355/358 of 3m6iA
- active site: C49 (= C37), G50 (= G38), S51 (= S39), H54 (= H42), H74 (= H62), E75 (= E63), C104 (= C92), C107 (= C95), C110 (= C98), C118 (= C106), D122 (≠ R110), P160 (= P153), A164 (≠ C157), K352 (= K338)
- binding nicotinamide-adenine-dinucleotide: C49 (= C37), V163 (= V156), G185 (= G178), P186 (= P179), I187 (= I180), D207 (= D200), R212 (≠ P205), C255 (≠ A243), T256 (≠ S244), I278 (≠ L266), G279 (= G267), V280 (≠ L268), R304 (= R291)
- binding zinc ion: C49 (= C37), H74 (= H62), C104 (= C92), C107 (= C95), C110 (= C98), C118 (= C106)
Q7SI09 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 2 papers)
32% identity, 99% coverage: 4:341/343 of query aligns to 12:359/363 of Q7SI09
- C53 (= C37) binding Zn(2+)
- F59 (≠ Y43) mutation F->A,S,Y: No effect.
- H78 (= H62) binding Zn(2+)
- E79 (= E63) binding Zn(2+)
- C108 (= C92) binding Zn(2+)
- C111 (= C95) binding Zn(2+)
- C114 (= C98) binding Zn(2+)
- C122 (= C106) binding Zn(2+)
- E163 (= E152) binding Zn(2+)
- PI 190:191 (= PI 179:180) binding NAD(+)
- D211 (= D200) binding NAD(+)
- DI 211:212 (≠ DL 200:201) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-348.
- R216 (≠ P205) binding NAD(+)
- I282 (≠ L266) binding NAD(+)
- QYR 306:308 (≠ TFR 289:291) binding NAD(+)
- S348 (≠ A331) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 211-SR-212.
2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
32% identity, 92% coverage: 16:329/343 of query aligns to 19:332/346 of 2dfvA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H65 (= H62), E66 (= E63), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), K113 (≠ R110), P151 (= P153), A155 (≠ C157)
- binding nicotinamide-adenine-dinucleotide: G175 (= G178), P176 (= P179), L177 (≠ I180), E197 (≠ D200), P198 (≠ L201), R202 (≠ P205), F241 (≠ A243), S242 (= S244), A244 (≠ S246), L264 (= L266), G265 (= G267), L266 (= L268), I289 (≠ T289), T290 (≠ F290)
- binding zinc ion: C95 (= C92), C101 (= C98), C109 (= C106)
Sites not aligning to the query:
O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
32% identity, 92% coverage: 16:329/343 of query aligns to 21:334/348 of O58389
- C42 (= C37) binding Zn(2+)
- T44 (≠ S39) mutation to A: Total loss of enzymatic activity.
- H67 (= H62) binding Zn(2+)
- E68 (= E63) binding Zn(2+)
- C97 (= C92) binding Zn(2+)
- C100 (= C95) binding Zn(2+)
- C103 (= C98) binding Zn(2+)
- C111 (= C106) binding Zn(2+)
- E152 (= E152) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
- L179 (≠ I180) binding NAD(+)
- E199 (≠ D200) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- R204 (≠ P205) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- LGL 266:268 (= LGL 266:268) binding NAD(+)
- IT 291:292 (≠ TF 289:290) binding NAD(+)
- R294 (≠ F292) mutation to A: 4000-fold decrease in catalytic efficiency.
Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
32% identity, 90% coverage: 22:329/343 of query aligns to 27:334/350 of Q5JI69
- L179 (≠ I180) binding NAD(+)
- E199 (≠ D200) binding NAD(+)
- R204 (≠ P205) binding NAD(+)
- LGL 266:268 (= LGL 266:268) binding NAD(+)
- IT 291:292 (≠ TF 289:290) binding NAD(+)
2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
32% identity, 99% coverage: 1:341/343 of query aligns to 1:339/343 of 2ejvA
- active site: C38 (= C37), G39 (= G38), T40 (≠ S39), H43 (= H42), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C99 (= C98), C107 (= C106), Q111 (≠ R110), P149 (= P153), A153 (≠ C157), K336 (= K338)
- binding nicotinamide-adenine-dinucleotide: G172 (= G176), G174 (= G178), P175 (= P179), I176 (= I180), S195 (≠ T199), D196 (= D200), P197 (≠ L201), R201 (≠ P205), F238 (≠ A243), S239 (= S244), N241 (≠ S246), A244 (= A249), L261 (= L266), G262 (= G267), I263 (≠ L268)
- binding zinc ion: C38 (= C37), H63 (= H62), E64 (= E63), C96 (= C95), C99 (= C98), C107 (= C106)
Query Sequence
>WP_090560791.1 NCBI__GCF_900101615.1:WP_090560791.1
MKSVVIHAPKDLRIEEAAAVAPGAGQVAIRIKAGGICGSDLHYYLHGGFGAVRLREPMVL
GHEIAGIVEALGEGVQGLSPGQAVAVNPSLPCGACRQCLAGRPNHCLDMRFYGSAMRMPH
VQGGFSELLVCEAERAVPLPAGMAPALAAFAEPLAVCLHAARQAGPMLGARVLVTGAGPI
GNLAIAVARHAGAREVVVTDLHSTPLLTARRMGADRTLSMSEDPDGLLPYAIDKGYFDLV
FEASGSGAALASAIQVARPGAVIVQLGLGGDVGLPLSALVAKEVALRGTFRFHEEFAQAV
EVLARGAIDPRPLLTATMPMADAVAAFELAADRSRAMKVQLAF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory