SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_090660499.1 NCBI__GCF_900101615.1:WP_090660499.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3u9sF Crystal structure of p. Aeruginosa 3-methylcrotonyl-coa carboxylase (mcc) 750 kd holoenzyme, coa complex (see paper)
71% identity, 100% coverage: 1:535/535 of query aligns to 3:537/537 of 3u9sF

query
sites
3u9sF

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active site: A150 (= A148), A192 (= A190), G193 (= G191), F381 (= F379), A421 (= A419), G422 (= G420)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T379 (≠ A377), G380 (= G378), F381 (= F379), A421 (= A419), M447 (= M445)
binding coenzyme a: R49 (= R47), K115 (= K113), T118 (≠ S116), A150 (= A148), N151 (= N149), L152 (= L150)

8xl8B Structure of human 3-methylcrotonyl-coa carboxylase in complex with propionyl-coa (mcc-pco)
65% identity, 99% coverage: 4:535/535 of query aligns to 9:541/541 of 8xl8B

query
sites
8xl8B

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binding propionyl Coenzyme A: K119 (= K113), G120 (= G114), G152 (= G146), A154 (= A148), A196 (= A190)
binding biotin: G384 (= G378), Q455 (= Q449), N458 (≠ S452)

8xl6B Structure of human 3-methylcrotonyl-coa carboxylase at apo-state (mcc- apo)
65% identity, 99% coverage: 4:535/535 of query aligns to 9:541/541 of 8xl6B

query
sites
8xl6B

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G

G

A

A

Y

Y

V

V

P

P

A

A

M

M

C

A

D

D

E

E

A

N

V

I

I

I

V

V

K

R

E

K

Q

Q

G

G

T

T

I

I

F

F

L

L

G

A

G

G

P

P

P

P

L

L

V

V

K

K

A

A

A

A

T

T

G

G

E

E

V

E

V

V

T

S

A

A

E

E

D

D

L

L

G

G

G

G

A

A

D

D

V

L

H

H

T

C

R

R

L

K

S

S

G

G

V

V

A

S

D

D

H

H

F

W

A

A

L

L

D

D

D

D

M

H

H

H

A

A

L

L

G

H

L

L

A

T

R

R

R

K

I

V

L

V

G

R

R

N

L

L

N

N

R

Y

R

Q

R

K

E

K

A

L

R

D

L

V

D

T

L

I

A

E

A

P

P

S

V

E

A

E

P

P

R

L

Y

F

D

P

P

A

A

D

E

E

L

L

H

Y

G

G

I

I

I

V

P

G

Q

A

D

N

V

L

R

K

Q

R

P

S

Y

F

D

D

V

V

R

R

E

E

V

V

I

I

A

A

R

R

I

I

V

V

D

D

G

G

S

S

E

R

L

F

D

T

E

E

F

F

K

K

A

A

R

F

Y

Y

G

G

T

D

T

T

L

L

V

V

C

T

G

G

F

F

A

A

R

R

I

I

W

F

G

G

H

Y

P

P

V

V

G

G

I

I

L

V

A

G

N

N

N

N

G

G

I

V

L

L

F

F

S

S

E

E

S

S

A

A

Q

K

K

K

G

G

A

T

H

H

F

F

I

V

E

Q

L

L

C

C

C

C

Q

Q

R

R

G

N

I

I

P

P

L

L

L

L

F

F

L

L

Q

Q

N

N

I

I

A

T

G
|
G

F

F

M

M

V

V

G

G

R

R

K

E

Y

Y

E

E

T

A

G

E

G

G

I

I

A

A

K

K

D

D

G

G

A

A

K

K

L

M

V

V

T

A

A

A

V

V

A

A

T

C

A

A

A

Q

V

V

P

P

K

K

F

I

T

T

V

L

L

I

I

I

G

G

G

G

S

S

F

Y

G

G

A

A

G

G

N

N

Y

Y

G

G

M

M

C

C

G

G

R

R

A

A

Y

Y

S

S

P

P

R

R

L

F

L

L

F

Y

T

I

W

W

P

P

N

N

S

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E

E

Q
|
Q

A

A

A

A

S
x
N

V

V

L

L

A

A

T

T

L

I

R

T

R

K

D

D

N

Q

I

R

E

A

A

R

K

E

G

G

G

K

N

Q

W

F

S

S

A

S

E

A

E

D

E

E

D

A

A

A

F

L

K

K

S

E

P

P

I

I

R

I

A

K

Q

K

Y

F

E

E

A

E

Q

E

G

G

S

N

P

P

Y

Y

Y

Y

A

S

T

S

A

A

R

R

L

V

W

W

D

D

D

D

G

G

I

I

I

I

D

D

P

P

A

A

L

D

T

T

R

R

D

L

V

V

L

L

G

G

L

L

G

S

L

F

A

S

A

A

A

A

C

L

N

N

A

A

P

P

I

I

G

E

E

K

T

T

R

D

F

F

G

G

V

I

F

F

R

R

M

M

binding biotin: G384 (= G378), Q455 (= Q449), N458 (≠ S452)

8k2vG 3-methylcrotonyl-coa carboxylase in mccd state with acetyl coa (see paper)
65% identity, 99% coverage: 4:535/535 of query aligns to 9:541/541 of 8k2vG

query
sites
8k2vG

L

L

A

G

S

T

S

Q

I

P

D

D

T

L

R

G

G

S

A

A

E

L

F

Y

R

Q

A

E

N

N

A

Y

E

K

A

Q

M

M

R

K

G

A

L

L

L

V

A

N

D

Q

L

L

A

H

D

E

R

R

T

V

A

E

Q

H

A

I

A

K

Q

L

G

G

G

G

P

G

E

E

A

K

A

A

R

R

Q

A

R

L

H

H

A

I

S

S

R

R

G

G

K

K

L

L

L

L

P

P

R

R

E

E

R

R

V

I

E

D

R

N

L

L

L

I

D

D

P

P

G

G

A

S

P

P

F

F

L

L

E

E

L

L

S

S

P

Q

L

F

A

A

A

G

H

Y

G

Q

M

L

Y

Y

G

D

G

N

D

E

-

E

V

V

P

P

A

G

G

G

G

G

I

I

L

I

T

T

G

G

I

I

G

G

R

R

V

V

S

S

G

G

Q

V

E

E

C

C

V

M

I

I

V

I

A

A

N

N

D

D

A

A

T

T

V

V

K
|
K

G
|
G

G

G

S

A

Y

Y

Y

Y

P

P

V

V

T

T

V

V

K

K

K

K

H

Q

L

L

R

R

A

A

Q

Q

E

E

I

I

A

A

L

M

Q

Q

N

N

H

R

L

L

P

P

C

C

V

I

Y

Y

L

L

V

V

D

D

S

S

G
|
G

G
|
G

A
|
A

N

Y

L
|
L

P

P

N

R

Q

Q

D

A

E

D

I

V

F

F

P

P

D

D

R

R

E

D

H

H

F

F

G

G

R

R

I

T

F

F

Y

Y

N

N

Q

Q

A

A

N

I

M

M

S

S

A

S

L

K

G

N

I

I

P

A

Q

Q

I

I

A

A

V

V

V

V

M

M

G

G

S

S

C

C

T
|
T

A

A

G

G

G

G

A

A

Y

Y

V

V

P

P

A

A

M

M

C

A

D

D

E

E

A

N

V

I

I

I

V

V

K

R

E

K

Q

Q

G

G

T

T

I

I

F

F

L

L

G

A

G

G

P

P

P

P

L

L

V

V

K

K

A

A

A

A

T

T

G

G

E

E

V

E

V

V

T

S

A

A

E

E

D

D

L

L

G

G

G

G

A

A

D

D

V

L

H

H

T

C

R

R

L

K

S

S

G

G

V

V

A

S

D

D

H

H

F

W

A

A

L

L

D

D

D

D

M

H

H

H

A

A

L

L

G

H

L

L

A

T

R

R

R

K

I

V

L

V

G

R

R

N

L

L

N

N

R

Y

R

Q

R

K

E

K

A

L

R

D

L

V

D

T

L

I

A

E

A

P

P

S

V

E

A

E

P

P

R

L

Y

F

D

P

P

A

A

D

E

E

L

L

H

Y

G

G

I

I

I

V

P

G

Q

A

D

N

V

L

R

K

Q

R

P

S

Y

F

D

D

V

V

R

R

E

E

V

V

I

I

A

A

R

R

I

I

V

V

D

D

G

G

S

S

E

R

L

F

D

T

E

E

F

F

K

K

A

A

R

F

Y

Y

G

G

T

D

T

T

L

L

V

V

C

T

G

G

F

F

A

A

R

R

I

I

W

F

G

G

H

Y

P

P

V

V

G

G

I

I

L

V

A

G

N

N

N

N

G

G

I

V

L

L

F

F

S

S

E

E

S

S

A

A

Q

K

K

K

G

G

A

T

H

H

F

F

I

V

E

Q

L

L

C

C

C

C

Q

Q

R

R

G

N

I

I

P

P

L

L

L

L

F

F

L

L

Q

Q

N

N

I

I

A

T

G

G

F
|
F

M

M

V
|
V

G

G

R

R

K

E

Y

Y

E

E

T

A

G

E

G

G

I

I

A

A

K

K

D

D

G

G

A

A

K

K

L

M

V

V

T

A

A

A

V

V

A

A

T

C

A

A

A

Q

V

V

P

P

K

K

F

I

T

T

V

L

L

I

I

I

G

G

G

G

S

S

F

Y

G

G

A

A

G

G

N

N

Y

Y

G

G

M

M

C

C

G

G

R

R

A

A

Y

Y

S

S

P

P

R

R

L

F

L

L

F

Y

T

I

W

W

P

P

N

N

S

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E

E

Q

Q

A

A

A

A

S

N

V

V

L

L

A

A

T

T

L

I

R

T

R

K

D

D

N

Q

I

R

E

A

A

R

K

E

G

G

G

K

N

Q

W

F

S

S

A

S

E

A

E

D

E

E

D

A

A

A

F

L

K

K

S

E

P

P

I

I

R

I

A

K

Q

K

Y

F

E

E

A

E

Q

E

G

G

S

N

P

P

Y

Y

Y

Y

A

S

T

S

A

A

R

R

L

V

W

W

D

D

D

D

G

G

I

I

I

I

D

D

P

P

A

A

L

D

T

T

R

R

D

L

V

V

L

L

G

G

L

L

G

S

L

F

A

S

A

A

A

A

C

L

N

N

A

A

P

P

I

I

G

E

E

K

T

T

R

D

F

F

G

G

V

I

F

F

R

R

M

M

binding acetyl coenzyme *a: K119 (= K113), G120 (= G114), G152 (= G146), G153 (= G147), A154 (= A148), L156 (= L150), T195 (= T189)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: F385 (= F379), V387 (= V381)

8j4zJ Human 3-methylcrotonyl-coa carboxylase in bccp-cts state with substrate (see paper)
65% identity, 99% coverage: 4:535/535 of query aligns to 9:541/541 of 8j4zJ

query
sites
8j4zJ

L

L

A

G

S

T

S

Q

I

P

D

D

T

L

R

G

G

S

A

A

E

L

F

Y

R

Q

A

E

N

N

A

Y

E

K

A

Q

M

M

R

K

G

A

L

L

L

V

A

N

D

Q

L

L

A

H

D

E

R

R

T

V

A

E

Q

H

A

I

A

K

Q

L

G

G

G

G

P

G

E

E

A

K

A

A

R

R

Q

A

R

L

H

H

A

I

S

S

R
|
R

G

G

K

K

L

L

L

L

P

P

R

R

E

E

R

R

V

I

E

D

R

N

L

L

L

I

D

D

P

P

G

G

A

S

P

P

F

F

L

L

E

E

L

L

S

S

P

Q

L

F

A

A

A

G

H

Y

G

Q

M

L

Y

Y

G

D

G

N

D

E

-

E

V

V

P

P

A

G

G

G

G

G

I

I

L

I

T

T

G

G

I

I

G

G

R

R

V

V

S

S

G

G

Q

V

E

E

C

C

V

M

I

I

V

I

A

A

N

N

D

D

A

A

T

T

V

V

K
|
K

G
|
G

G

G

S
x
A

Y

Y

Y

Y

P

P

V

V

T

T

V

V

K

K

K

K

H

Q

L

L

R

R

A

A

Q

Q

E

E

I

I

A

A

L

M

Q

Q

N

N

H

R

L

L

P

P

C

C

V

I

Y

Y

L

L

V

V

D

D

S

S

G
|
G

G
|
G

A
|
A

N
x
Y

L

L

P

P

N

R

Q

Q

D

A

E

D

I

V

F

F

P

P

D

D

R

R

E

D

H

H

F
|
F

G

G

R

R

I

T

F

F

Y

Y

N

N

Q

Q

A

A

N

I

M

M

S

S

A

S

L

K

G

N

I

I

P

A

Q

Q

I

I

A

A

V

V

V

V

M

M

G

G

S

S

C

C

T
|
T

A
|
A

G
|
G

G

G

A

A

Y

Y

V

V

P

P

A

A

M

M

C

A

D

D

E

E

A

N

V

I

I

I

V

V

K

R

E

K

Q

Q

G

G

T

T

I

I

F

F

L
|
L

G

A

G

G

P

P

P

P

L
|
L

V

V

K

K

A

A

A

A

T

T

G

G

E

E

V

E

V

V

T

S

A

A

E

E

D

D

L

L

G

G

G

G

A

A

D

D

V

L

H

H

T

C

R

R

L

K

S

S

G

G

V

V

A

S

D

D

H

H

F

W

A

A

L

L

D

D

D

D

M

H

H

H

A

A

L

L

G

H

L

L

A

T

R

R

R

K

I

V

L

V

G

R

R

N

L

L

N

N

R

Y

R

Q

R

K

E

K

A

L

R

D

L

V

D

T

L

I

A

E

A

P

P

S

V

E

A

E

P

P

R

L

Y

F

D

P

P

A

A

D

E

E

L

L

H

Y

G

G

I

I

I

V

P

G

Q

A

D

N

V

L

R

K

Q

R

P

S

Y

F

D

D

V

V

R

R

E

E

V

V

I

I

A

A

R

R

I

I

V

V

D

D

G

G

S

S

E

R

L

F

D

T

E

E

F

F

K

K

A

A

R

F

Y

Y

G

G

T

D

T

T

L

L

V

V

C

T

G

G

F

F

A

A

R

R

I

I

W

F

G

G

H

Y

P

P

V

V

G

G

I

I

L

V

A

G

N

N

N

N

G

G

I

V

L

L

F

F

S

S

E

E

S

S

A

A

Q

K

K

K

G

G

A

T

H

H

F

F

I

V

E

Q

L

L

C

C

C

C

Q

Q

R

R

G

N

I

I

P

P

L

L

L

L

F

F

L

L

Q

Q

N

N

I

I

A

T

G
|
G

F
|
F

M

M

V

V

G

G

R

R

K

E

Y

Y

E

E

T

A

G

E

G

G

I

I

A

A

K

K

D

D

G

G

A

A

K

K

L

M

V

V

T

A

A

A

V

V

A

A

T

C

A

A

A

Q

V

V

P

P

K

K

F

I

T

T

V

L

L

I

I

I

G

G

G

G

S

S

F
x
Y

G

G

A
|
A

G

G

N

N

Y

Y

G

G

M

M

C

C

G

G

R

R

A

A

Y

Y

S

S

P

P

R

R

L

F

L

L

F

Y

T

I

W

W

P

P

N

N

S

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E

E

Q

Q

A

A

A

A

S

N

V

V

L

L

A

A

T

T

L
x
I

R

T

R

K

D

D

N
x
Q

I

R

E

A

A

R

K

E

G

G

G

K

N

Q

W

F

S

S

A

S

E

A

E

D

E

E

D

A

A

A

F

L

K

K

S

E

P

P

I

I

R

I

A

K

Q

K

Y

F

E

E

A

E

Q

E

G

G

S

N

P

P

Y

Y

Y

Y

A

S

T

S

A

A

R

R

L

V

W

W

D

D

D

D

G

G

I

I

I

I

D

D

P

P

A

A

L

D

T

T

R

R

D

L

V

V

L

L

G

G

L

L

G

S

L

F

A

S

A

A

A

A

C

L

N

N

A

A

P

P

I

I

G

E

E

K

T

T

R

D

F

F

G

G

V

I

F

F

R

R

M

M

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: L219 (= L213), L224 (= L218), G384 (= G378), F385 (= F379), Y423 (≠ F417), A425 (= A419)
binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{S},3~{S},4~{S},5~{S})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 3-methylbut-2-enethioate: R56 (= R51), K119 (= K113), G120 (= G114), A122 (≠ S116), G152 (= G146), G153 (= G147), A154 (= A148), Y155 (≠ N149), F169 (= F163), T195 (= T189), A196 (= A190), G197 (= G191), I463 (≠ L457), Q467 (≠ N461)

8jxmC Human 3-methylcrotonyl-coa carboxylase in bccp-h2 state with mcoa (see paper)
61% identity, 99% coverage: 4:535/535 of query aligns to 9:520/520 of 8jxmC

query
sites
8jxmC

L

L

A

G

S

T

S

Q

I

P

D

D

T

L

R

G

G

S

A

A

E

L

F

Y

R

Q

A

E

N

N

A

Y

E

K

A

Q

M

M

R

K

G

A

L

L

L

V

A

N

D

Q

L

L

A

H

D

E

R

R

T

V

A

E

Q

H

A

I

A

K

Q

L

G

G

G

G

P

G

E

E

A

K

A

A

R

R

Q

A

R

L

H

H

A

I

S

S

R
|
R

G

G

K

K

L

L

L

L

P

P

R

R

E

E

R

R

V

I

E

D

R

N

L

L

L

I

D

D

P

P

G

G

A

S

P

P

F

F

L

L

E

E

L

L

S

S

P

Q

L

F

A

A

A

G

H

Y

G

Q

M

L

Y

Y

G

D

G

N

D

E

-

E

V

V

P

P

A

G

G

G

G

G

I

I

L

I

T

T

G

G

I

I

G

G

R

R

V

V

S

S

G

G

Q

V

E

E

C

C

V

M

I

I

V

I

A

A

N

N

D

D

A

A

T

T

V

V

K
|
K

G
|
G

G

G

S

A

Y

Y

Y

Y

P

P

V

V

T

T

V

V

K

K

K

K

H

Q

L

L

R

R

A

A

Q

Q

E

E

I

I

A

A

L

M

Q

Q

N

N

H

R

L

L

P

P

C

C

V

I

Y

Y

L

L

V

V

D

D

S

S

G
|
G

G

G

A
|
A

N
x
Y

L

L

P

P

N

R

Q

Q

D

A

E

D

I

V

F
|
F

P

P

D

D

R

R

E

D

H

H

F

F

G

G

R

R

I

T

F

F

Y

Y

N

N

Q

Q

A

A

N

I

M

M

S

S

A

S

L

K

G

N

I

I

P

A

Q

Q

I

I

A

A

V

V

V

V

M

M

G

G

S

S

C

C

T
|
T

A
|
A

G

G

G

G

A

A

Y

Y

V

V

P

P

A

A

M

M

C

A

D

D

E

E

A

N

V

I

I

I

V

V

K

R

E

K

Q

Q

G

G

T

T

I

I

F

-

L

-

G

-

G

-

P

-

P

-

L

-

V

-

K

-

A

-

A

-

T

-

G

-

E

-

V

-

V

-

T

-

A

-

E

-

D

-

L

-

G

G

G

G

A

A

D

D

V

L

H

H

T

C

R

R

L

K

S

S

G

G

V

V

A

S

D

D

H

H

F

W

A

A

L

L

D

D

D

D

M

H

H

H

A

A

L

L

G

H

L

L

A

T

R

R

R

K

I

V

L

V

G

R

R

N

L

L

N

N

R

Y

R

Q

R

K

E

K

A

L

R

D

L

V

D

T

L

I

A

E

A

P

P

S

V

E

A

E

P

P

R

L

Y

F

D

P

P

A

A

D

E

E

L

L

H

Y

G

G

I

I

I

V

P

G

Q

A

D

N

V

L

R

K

Q

R

P

S

Y

F

D

D

V

V

R

R

E

E

V

V

I

I

A

A

R

R

I

I

V

V

D

D

G

G

S

S

E

R

L

F

D

T

E

E

F

F

K

K

A

A

R

F

Y

Y

G

G

T

D

T

T

L

L

V

V

C

T

G

G

F

F

A

A

R

R

I

I

W

F

G

G

H

Y

P

P

V

V

G

G

I

I

L

V

A

G

N

N

N

N

G

G

I

V

L

L

F

F

S

S

E

E

S

S

A

A

Q

K

K

K

G

G

A

T

H

H

F

F

I

V

E

Q

L

L

C

C

C

C

Q

Q

R

R

G

N

I

I

P

P

L

L

L

L

F

F

L

L

Q

Q

N

N

I

I

A
x
T

G
|
G

F
|
F

M

M

V

V

G

G

R

R

K

E

Y

Y

E

E

T

A

G

E

G

G

I

I

A

A

K

K

D

D

G

G

A

A

K

K

L

M

V

V

T

A

A

A

V

V

A

A

T

C

A

A

A

Q

V

V

P

P

K

K

F

I

T

T

V

L

L

I

I

I

G

G

G

G

S

S

F
x
Y

G
|
G

A
|
A

G

G

N

N

Y

Y

G

G

M

M

C

C

G

G

R

R

A

A

Y

Y

S

S

P

P

R

R

L

F

L

L

F

Y

T

I

W

W

P

P

N

N

S

A

R

R

I

I

S

S

V

V

M
|
M

G

G

G

G

E

E

Q

Q

A

A

A

A

S

N

V

V

L

L

A

A

T

T

L
x
I

R

T

R

K

D

D

N
x
Q

I

R

E

A

A

R

K

E

G

G

G

K

N

Q

W

F

S

S

A

S

E

A

E

D

E

E

D

A

A

A

F

L

K

K

S

E

P

P

I

I

R

I

A

K

Q

K

Y

F

E

E

A

E

Q

E

G

G

S

N

P

P

Y

Y

Y

Y

A

S

T

S

A

A

R

R

L

V

W

W

D

D

D

D

G

G

I

I

I

I

D

D

P

P

A

A

L

D

T

T

R

R

D

L

V

V

L

L

G

G

L

L

G

S

L

F

A

S

A

A

A

A

C

L

N

N

A

A

P

P

I

I

G

E

E

K

T

T

R

D

F

F

G

G

V

I

F

F

R

R

M

M

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T362 (≠ A377), G363 (= G378), F364 (= F379), Y402 (≠ F417), G403 (= G418), A404 (= A419), M430 (= M445)
binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{S},3~{S},4~{S},5~{S})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 3-methylbut-2-enethioate: R56 (= R51), K119 (= K113), G120 (= G114), G152 (= G146), A154 (= A148), Y155 (≠ N149), F163 (= F157), T195 (= T189), A196 (= A190), I442 (≠ L457), Q446 (≠ N461)

8rthF Trypanosoma brucei 3-methylcrotonyl-coa carboxylase (see paper)
62% identity, 98% coverage: 2:524/535 of query aligns to 20:542/542 of 8rthF

query
sites
8rthF

S

N

I

V

L

L

A

H

S

Y

S

P

I

A

D

E

T

S

R

T

G

N

A

P

E

E

F

F

R

K

A

A

N

N

A

T

E

E

A

R

M

M

R

K

G

A

L

L

L

T

A

A

D

E

L

L

A

R

D

R

R

R

T

V

A

Q

Q

V

A

I

A

V

Q

D

G

G

G

D

P

S

E

E

A

A

-

D

-

K

-

R

A

A

R

R

Q

D

R

R

H

H

A

I

S

S

R

R

G

G

K

K

L

L

L

L

P

V

R

H

E

Q

R

R

V

I

E

E

R

K

L

L

L

V

D

D

P

P

G

M

A

S

P

P

F

F

L

L

E

E

L

L

S

S

P

Q

L

L

A

A

A

G

H

G

G

D

M

L

Y

Y

G

P

G

G

D

E

-

A

V

C

P

H

A

R

G

G

G

G

I

I

L

L

T

T

G

G

I

I

G

G

R

V

V

V

S

H

G

G

Q

M

E

R

C

V

V

M

I

I

V

V

A

A

N

N

D

D

A

A

T

T

V

V

K

K

G

G

G

G

S

T

Y

Y

Y

Y

P

P

V

I

T

T

V

V

K

K

K

K

H

H

L

L

R

R

A

A

Q

Q

E

R

I

I

A

A

L

E

Q

E

N

N

H

R

L

L

P

P

C

C

V

I

Y

Y

L

L

V

V

D

D

S

S

G

G

G

G

A

A

N

N

L

L

P

G

N

M

Q

Q

D

G

E

D

I

V

F

F

P

P

D

D

R

E

E

Q

H

H

F

F

G

G

R

R

I

I

F

F

Y

F

N

N

Q

Q

A

A

N

N

M

M

S

S

A

A

L

K

G

G

I

I

P

A

Q

Q

I

I

A

A

V

T

V

V

M

M

G

G

S

S

C

C

T

T

A

A

G

G

G

G

A

A

Y

Y

V

V

P

P

A

A

M

M

C

S

D

D

E

E

A

S

V

I

I

I

V

V

K

K

E

G

Q

N

G

G

T

T

I

I

F

F

L

L

G

G

G

G

P

P

P

P

L

L

V

V

K

F

A

A

A

A

T

T

G

G

E

E

V

E

V

V

T

T

A

P

E

E

D

E

L

L

G

G

G

G

A

A

D

D

V

V

H

H

T

C

R

R

L

A

S

S

G

G

V

V

A

T

D

D

H

Y

F

F

A

A

L

T

D

D

D

D

M

L

H

H

A

A

L

L

G

Y

L

L

A

T

R

R

R

R

I

I

L

V

G

A

R

N

L

L

N

N

R

R

-

N

-

D

-

C

R

E

R

R

E

P

A

C

R

R

L

G

D

R

L

E

A

F

A

T

P

P

V

-

A

-

P

P

R

L

Y

Y

D

D

P

P

A

S

E

E

L

I

H

G

G

G

I

F

I

I

P

P

Q

D

-

M

-

G

-

A

D

D

V

V

R

V

Q

K

P

G

Y

F

D

D

V

V

R

R

E

A

V

V

I

I

A

A

R

R

I

L

V

V

D

D

G

G

S

S

E

E

L

F

D

D

E

E

F

F

K

K

A

K

R

L

Y

Y

G

G

T

D

T

T

L

L

V

V

C

C

G

G

F

F

A

A

R

R

I

F

W

E

G

G

H

M

P

L

V

V

G

G

I

I

L

V

A

A

N

N

N

N

G

G

I

I

L

L

F

Y

S

S

E

E

S

S

A

A

Q

L

K

K

G

G

A

A

H

H

F

F

I

V

E

E

L

L

C

C

C

S

Q

H

R

R

G

N

I

I

P

P

L

L

L

L

F

F

L

L

Q

Q

N

N

I

I

A
x
T

G
|
G

F
|
F

M

M

V

V

G

G

R

K

K

T

Y

Y

E

E

T

E

G

G

G

G

I

I

A

A

K

K

D

N

G

G

A

A

K

K

L

L

V

V

T

T

A

A

V

V

A

S

T

T

A

T

A

H

V

V

P

P

K

K

F

I

T

T

V

I

L

I

I

I

G

G

G

G

S

S

F

Y

G

G

A

A

G

G

N

N

Y

Y

G

G

M

M

C

C

G

G

R

R

A

A

Y

F

S

G

P

P

R

R

L

F

L

L

F

F

T

M

W

W

P

P

N

N

S

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E
x
N

Q
|
Q

A

A

A

A

S

T

V

V

L

L

A

A

T

L

L

-

R

-

R

-

D

-

N

-

I

-

E

-

A

-

K

T

G

N

G

S

N

K

W

L

S

R

A

E

E

N

E

E

E

V

D

Q

A

D

F

F

K

K

S

A

P

K

I

V

R

R

A

S

Q

K

Y

Y

E

E

A

Y

Q

E

G

G

S

S

P

C

Y

Y

Y

Y

A

S

T

T

A

A

R

R

L

L

W

W

D

D

D

D

G

G

I

V

I

I

D

A

P

P

A

E

L

D

T

T

R

R

D

A

V

V

L

V

G

V

L

Q

G

A

L

L

A

L

A

S

A

T

C

L

N

S

A

A

P

P

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T403 (≠ A377), G404 (= G378), F405 (= F379), N474 (≠ E448), Q475 (= Q449)

8f3dA 3-methylcrotonyl-coa carboxylase in filament, beta-subunit centered (see paper)
60% identity, 98% coverage: 2:524/535 of query aligns to 17:566/566 of 8f3dA

query
sites
8f3dA

S

N

I

I

L

L

A

R

S

L

S

P

I

A

D

N

T

T

R

S

G

D

A

P

E

T

F

Y

R

Q

A

E

N

N

A

M

E

A

A

R

M

M

R

E

G

G

L

L

L

V

A

E

D

Q

L

L

A

R

D

A

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|
F

M

M

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V

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|
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A

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N

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P

P

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: F426 (= F379), Q496 (= Q449)

1on3E Transcarboxylase 12s crystal structure: hexamer assembly and substrate binding to a multienzyme core (with methylmalonyl-coenzyme a and methylmalonic acid bound) (see paper)
36% identity, 92% coverage: 19:510/535 of query aligns to 6:493/520 of 1on3E

query
sites
1on3E

A

A

E

S

A

T

M

M

R

E

G

G

L

R

L

V

A

E

D

Q

L

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A

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G

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G

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A

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A
x
R

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Q

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G

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K

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N

R

N

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L

L

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D

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P

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F

F

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x
F

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M

G
|
G

G

G

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|
S

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A

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F

V

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Y

F

L

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Y

D

D

S

S

G
|
G

G

G

A
|
A

N

R

L
x
I

P

-

N

-

Q

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D

Q

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G

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|
G

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