SitesBLAST
Comparing WP_090664325.1 NCBI__GCF_900101615.1:WP_090664325.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
41% identity, 100% coverage: 1:250/251 of query aligns to 3:254/255 of 5itvA
- active site: G18 (= G16), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), S17 (= S15), G18 (= G16), I19 (= I17), D38 (= D36), I39 (≠ V37), T61 (= T59), I63 (≠ V61), N89 (≠ D87), G91 (≠ A89), T139 (≠ I142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (= G188), I186 (≠ K189), I187 (= I190)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
41% identity, 100% coverage: 1:250/251 of query aligns to 3:226/227 of 5itvD
- active site: G18 (= G16), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), S17 (= S15), G18 (= G16), I19 (= I17), D38 (= D36), I39 (≠ V37), T61 (= T59), D62 (= D60), I63 (≠ V61), N89 (≠ D87), T139 (≠ I142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (= G188), I187 (= I190)
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
37% identity, 99% coverage: 3:251/251 of query aligns to 11:259/267 of 3ay6B
- active site: G24 (= G16), S151 (= S144), Y164 (= Y157), K168 (= K161)
- binding beta-D-glucopyranose: E102 (≠ T91), S151 (= S144), H153 (= H146), W158 (≠ S151), Y164 (= Y157), N202 (≠ T195), K205 (≠ A198)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G12), T23 (≠ S15), G24 (= G16), L25 (≠ I17), Y45 (vs. gap), D71 (= D60), V72 (= V61), N98 (≠ D87), A99 (= A88), G100 (≠ A89), V101 (≠ I90), M149 (≠ I142), S151 (= S144), Y164 (= Y157), K168 (= K161), P194 (= P187), G195 (= G188), M197 (≠ I190), T199 (= T192), P200 (≠ G193), I201 (≠ K194), N202 (≠ T195)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
37% identity, 99% coverage: 1:249/251 of query aligns to 1:246/247 of 4jroC
- active site: G16 (= G16), S142 (= S144), Q152 (≠ D154), Y155 (= Y157), K159 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ S15), G16 (= G16), I17 (= I17), N35 (vs. gap), Y36 (vs. gap), N37 (vs. gap), G38 (vs. gap), S39 (vs. gap), N63 (≠ D60), V64 (= V61), N90 (≠ D87), A91 (= A88), I93 (= I90), I113 (≠ V110), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), I188 (= I190), T190 (= T192)
2cfcA Structural basis for stereo selectivity in the (r)- and (s)- hydroxypropylethane thiosulfonate dehydrogenases (see paper)
43% identity, 98% coverage: 6:250/251 of query aligns to 3:249/250 of 2cfcA
- active site: G13 (= G16), S142 (= S144), Y155 (= Y157), K159 (= K161)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ S151), R152 (≠ D154), Y155 (= Y157), W195 (≠ R197), R196 (≠ A198)
- binding nicotinamide-adenine-dinucleotide: G9 (= G12), S12 (= S15), G13 (= G16), N14 (≠ I17), D33 (= D36), L34 (≠ V37), A59 (≠ T59), D60 (= D60), V61 (= V61), N87 (≠ D87), A88 (= A88), G89 (≠ A89), I140 (= I142), P185 (= P187), G186 (= G188), M187 (≠ K189), I188 (= I190), T190 (= T192), P191 (≠ G193), M192 (≠ K194), T193 (= T195)
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
43% identity, 98% coverage: 6:250/251 of query aligns to 3:249/250 of Q56840
- SGN 12:14 (≠ SGI 15:17) binding NAD(+)
- D33 (= D36) binding NAD(+)
- DV 60:61 (= DV 60:61) binding NAD(+)
- N87 (≠ D87) binding NAD(+)
- S142 (= S144) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (≠ D154) binding 2-oxopropyl-coenzyme M; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y157) mutation Y->E,F: Loss of activity.
- K159 (= K161) mutation to A: Loss of activity.
- R179 (≠ V181) mutation to A: Loss of activity.
- IETPM 188:192 (≠ ILTGK 190:194) binding NAD(+)
- WR 195:196 (≠ RA 197:198) binding 2-oxopropyl-coenzyme M
- R196 (≠ A198) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- R203 (≠ I204) mutation to A: Slight decrease in catalytic efficiency.
- R209 (= R210) mutation to A: Does not affect catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
4nbuB Crystal structure of fabg from bacillus sp (see paper)
39% identity, 99% coverage: 2:249/251 of query aligns to 4:243/244 of 4nbuB
- active site: G18 (= G16), N111 (= N111), S139 (= S144), Q149 (≠ D154), Y152 (= Y157), K156 (= K161)
- binding acetoacetyl-coenzyme a: D93 (≠ G93), K98 (≠ D98), S139 (= S144), N146 (≠ S151), V147 (≠ P152), Q149 (≠ D154), Y152 (= Y157), F184 (≠ K189), M189 (≠ K194), K200 (≠ Y206)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ S15), G18 (= G16), I19 (= I17), D38 (= D36), F39 (≠ V37), V59 (≠ T59), D60 (= D60), V61 (= V61), N87 (≠ D87), A88 (= A88), G89 (≠ A89), I90 (= I90), T137 (≠ I142), S139 (= S144), Y152 (= Y157), K156 (= K161), P182 (= P187), F184 (≠ K189), T185 (≠ I190), T187 (= T192), M189 (≠ K194)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
38% identity, 99% coverage: 1:248/251 of query aligns to 1:241/244 of 6t77A
- active site: G16 (= G16), S138 (= S144), Y151 (= Y157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ S15), T37 (≠ V40), L58 (≠ T59), N59 (≠ D60), V60 (= V61), A87 (= A88), G88 (≠ A89), I89 (= I90)
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
43% identity, 98% coverage: 3:247/251 of query aligns to 3:236/240 of 4dmmB
- active site: G16 (= G16), S142 (= S144), Q152 (≠ D154), Y155 (= Y157), K159 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ S15), G16 (= G16), I17 (= I17), A37 (≠ V37), S38 (≠ T38), S39 (≠ T39), A62 (≠ T59), D63 (= D60), V64 (= V61), N90 (≠ D87), A91 (= A88), L113 (≠ V110), I140 (= I142), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), G186 (= G188), I188 (= I190), T190 (= T192), M192 (= M203)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
37% identity, 99% coverage: 1:248/251 of query aligns to 1:241/244 of P0AEK2
- GASR 12:15 (≠ GGAS 12:15) binding NADP(+)
- T37 (≠ V40) binding NADP(+)
- NV 59:60 (≠ DV 60:61) binding NADP(+)
- N86 (≠ D87) binding NADP(+)
- Y151 (= Y157) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YGTSK 157:161) binding NADP(+)
- A154 (≠ S160) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K161) mutation to A: Defect in the affinity for NADPH.
- I184 (= I190) binding NADP(+)
- E233 (= E240) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
37% identity, 98% coverage: 2:248/251 of query aligns to 1:240/243 of 1q7bA
- active site: G15 (= G16), E101 (= E103), S137 (= S144), Q147 (≠ D154), Y150 (= Y157), K154 (= K161)
- binding calcium ion: E232 (= E240), T233 (≠ N241)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (≠ S15), T36 (≠ V40), N58 (≠ D60), V59 (= V61), N85 (≠ D87), A86 (= A88), G87 (≠ A89), I88 (= I90), S137 (= S144), Y150 (= Y157), K154 (= K161), P180 (= P187), G181 (= G188), I183 (= I190)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
36% identity, 99% coverage: 1:248/251 of query aligns to 4:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (≠ S15), I20 (= I17), T40 (≠ V40), N62 (≠ D60), V63 (= V61), N89 (≠ D87), A90 (= A88), I92 (= I90), V139 (≠ I142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (= G188), I187 (= I190), T189 (= T192), M191 (≠ K194)
Q5P5I4 (S)-1-Phenylethanol dehydrogenase; EC 1.1.1.311 from Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) (see 2 papers)
38% identity, 98% coverage: 2:247/251 of query aligns to 4:245/249 of Q5P5I4
- NGI 17:19 (≠ SGI 15:17) binding NAD(+)
- D38 (= D36) binding NAD(+)
- CDV 61:63 (≠ TDV 59:61) binding NAD(+)
- N89 (≠ D87) binding NAD(+)
- Y93 (≠ T91) binding NAD(+)
- K158 (= K161) binding NAD(+)
- PSLV 184:187 (≠ PGKI 187:190) binding NAD(+)
- T191 (≠ K194) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2ewmB Crystal structure of the (s)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain ebn1 (see paper)
38% identity, 98% coverage: 2:247/251 of query aligns to 2:243/247 of 2ewmB
- active site: G16 (= G16), S139 (= S144), Y149 (≠ D154), Y152 (= Y157), K156 (= K161)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), N15 (≠ S15), G16 (= G16), I17 (= I17), D36 (= D36), L37 (≠ V37), C59 (≠ T59), D60 (= D60), V61 (= V61), N87 (≠ D87), S139 (= S144), Y152 (= Y157), K156 (= K161), P182 (= P187), S183 (≠ G188), L184 (≠ K189), V185 (≠ I190), T189 (≠ K194)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 99% coverage: 1:248/251 of query aligns to 1:241/244 of P0A2C9
- M125 (= M126) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A230) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S231) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
37% identity, 98% coverage: 2:248/251 of query aligns to 1:240/243 of 1q7cA
- active site: G15 (= G16), S137 (= S144), Q147 (≠ D154), F150 (≠ Y157), K154 (= K161)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (≠ S15), A35 (≠ T39), T36 (≠ V40), L57 (≠ T59), N58 (≠ D60), V59 (= V61), G87 (≠ A89), I88 (= I90)
2d1yA Crystal structure of tt0321 from thermus thermophilus hb8 (see paper)
43% identity, 96% coverage: 9:250/251 of query aligns to 9:238/240 of 2d1yA