SitesBLAST
Comparing WP_091206026.1 NCBI__GCF_900110105.1:WP_091206026.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
36% identity, 96% coverage: 3:315/325 of query aligns to 68:385/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (≠ V115), R200 (vs. gap), M281 (≠ R211), G282 (= G212), R307 (≠ K237), A308 (≠ P238), Q320 (≠ H250), V321 (= V251), G322 (= G252), Q323 (= Q253), T324 (= T254), G337 (= G267), I338 (= I268), S339 (= S269), Q343 (= Q273), H344 (= H274), N358 (= N288), K359 (= K289), L377 (≠ A307)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 98% coverage: 2:318/325 of query aligns to 3:324/331 of 5ol2A
- binding calcium ion: E75 (≠ Q74), D188 (= D183)
- binding flavin-adenine dinucleotide: T117 (≠ A116), R136 (≠ K134), I147 (≠ V145), G216 (= G210), R217 (= R211), G218 (= G212), S242 (= S236), R243 (≠ K237), A244 (≠ P238), Q256 (≠ H250), V257 (= V251), G258 (= G252), T260 (= T254), G273 (= G267), I274 (= I268), S275 (= S269), A277 (= A271), Q279 (= Q273), H280 (= H274), N294 (= N288), K295 (= K289), D312 (= D306), V313 (≠ A307)
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
33% identity, 99% coverage: 2:324/325 of query aligns to 12:338/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (≠ V115), R144 (≠ K134), I155 (≠ V145), G224 (= G210), R225 (= R211), G226 (= G212), S250 (= S236), R251 (≠ K237), A252 (≠ P238), Q264 (≠ H250), V265 (= V251), G266 (= G252), Q267 (= Q253), S268 (≠ T254), G281 (= G267), I282 (= I268), S283 (= S269), S285 (≠ A271), Q287 (= Q273), H288 (= H274), N302 (= N288), K303 (= K289), D320 (= D306), A321 (= A307)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
34% identity, 98% coverage: 2:320/325 of query aligns to 21:332/333 of P13804
- G116 (= G100) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (≠ N159) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R211) binding FAD
- S248 (= S236) binding FAD
- R249 (≠ K237) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (= VGQT 251:254) binding FAD
- T266 (= T254) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (= SGAIQH 269:274) binding FAD
- N300 (= N288) binding FAD
- DL 318:319 (≠ DA 306:307) binding FAD
Sites not aligning to the query:
- 20:204 Domain I
- 205:333 Domain II
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
34% identity, 98% coverage: 2:320/325 of query aligns to 3:314/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G210), R205 (= R211), S230 (= S236), R231 (≠ K237), A232 (≠ P238), Q244 (≠ H250), V245 (= V251), G246 (= G252), T248 (= T254), G261 (= G267), I262 (= I268), S263 (= S269), A265 (= A271), Q267 (= Q273), H268 (= H274), N282 (= N288), K283 (= K289), D300 (= D306), L301 (≠ A307)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 98% coverage: 3:319/325 of query aligns to 13:332/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (≠ V115), T126 (≠ A116), R144 (≠ K134), I155 (≠ V145), R224 (= R211), G225 (= G212), T249 (≠ S236), R250 (≠ K237), Q263 (≠ H250), I264 (≠ V251), G265 (= G252), L266 (≠ Q253), S267 (≠ T254), G280 (= G267), I281 (= I268), S282 (= S269), Q286 (= Q273), N301 (= N288), S302 (≠ K289), D303 (= D290), D319 (= D306), L320 (≠ A307)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
42% identity, 67% coverage: 97:314/325 of query aligns to 91:303/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G210), R200 (= R211), G201 (= G212), S225 (= S236), R226 (≠ K237), A227 (≠ P238), Q239 (≠ H250), V240 (= V251), G241 (= G252), T243 (= T254), G256 (= G267), I257 (= I268), S258 (= S269), A260 (= A271), Q262 (= Q273), H263 (= H274), N277 (= N288), K278 (= K289), D295 (= D306), L296 (≠ A307)
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
34% identity, 78% coverage: 67:319/325 of query aligns to 47:292/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G210), R184 (= R211), G185 (= G212), S209 (= S236), R210 (≠ K237), Q223 (≠ H250), I224 (≠ V251), G225 (= G252), T227 (= T254), G240 (= G267), V241 (≠ I268), S242 (= S269), A244 (= A271), Q246 (= Q273), H247 (= H274), N261 (= N288), K262 (= K289), D279 (= D306), Y280 (≠ A307)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
33% identity, 98% coverage: 3:320/325 of query aligns to 7:329/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (vs. gap), R140 (≠ K134), T142 (≠ A136), G219 (= G210), K220 (≠ R211), G221 (= G212), S245 (= S236), R246 (≠ K237), A247 (≠ P238), Q259 (≠ H250), V260 (= V251), G261 (= G252), Q262 (= Q253), T263 (= T254), G276 (= G267), S278 (= S269), Q282 (= Q273), H283 (= H274), N297 (= N288), I298 (≠ K289), L316 (≠ A307)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
28% identity, 97% coverage: 3:318/325 of query aligns to 4:319/321 of P53571
- R211 (= R211) binding FAD
- SR 236:237 (≠ SK 236:237) binding FAD
- QVGQS 250:254 (≠ HVGQT 250:254) binding FAD
- 268:275 (vs. 267:274, 88% identical) binding FAD
- N289 (= N288) binding FAD
- DI 307:308 (≠ DA 306:307) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
28% identity, 97% coverage: 3:318/325 of query aligns to 3:318/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G210), R210 (= R211), G211 (= G212), S235 (= S236), A236 (≠ K237), P237 (= P238), Q249 (≠ H250), V250 (= V251), G251 (= G252), Q252 (= Q253), S253 (≠ T254), G267 (= G267), I268 (= I268), S269 (= S269), S271 (≠ A271), Q273 (= Q273), H274 (= H274), N288 (= N288), T289 (≠ K289), D306 (= D306), I307 (≠ A307)
Query Sequence
>WP_091206026.1 NCBI__GCF_900110105.1:WP_091206026.1
MSVLIYAENAGGKFKKSIFEAVSYARAIADQSNTSVTAVSIGNVDATELALLGKYGADKV
LNVSGDKLKDFVNQAYASVIAEAAKKEGSAVVVLSNTFSGRGLAPRVGVKLEAGVADGAV
ALPEQAGGSFKVKKTAFSGKAFATVELTAAIKVIALVPNSYKVVETGGTAQVEDFNVEPK
ASDFKAMIKEIVRSTDKVSLPDAEIVVSGGRGLKGPENWGMIEELAELLGAATACSKPVS
DAGWRPHSEHVGQTGIAVSPNLYIAVGISGAIQHLAGVSSSKVIVVINKDPEAPFFKVAD
YGIVGDAFEVLPKLIAAVKEYKASA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory