SitesBLAST

HLN 66:68 (= HLN 62:64) binding glycerol
Y138 (≠ G124) binding glycerol
GFA 199:201 (≠ GYA 189:191) binding glycerol
N203 (= N193) binding glycerol
R206 (= R196) binding glycerol
PL 236:237 (≠ PV 223:224) mutation to FW: No detectable water or glycerol permeability.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
34% identity, 98% coverage: 5:241/243 of query aligns to 7:249/254 of 1fx8A

query
sites
1fx8A

V

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A

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binding glycerol: W43 (= W44), N63 (= N64), G194 (= G189), F195 (≠ Y190), A196 (= A191), N198 (= N193), R201 (= R196)

O14520 Aquaporin-7; AQP-7; Aquaglyceroporin-7; Aquaporin adipose; AQPap; Aquaporin-7-like from Homo sapiens (Human) (see 4 papers)
33% identity, 97% coverage: 4:239/243 of query aligns to 37:273/342 of O14520

query
sites
O14520

F

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L

V59 (= V26) to L: in dbSNP:rs4008659
Y135 (= Y105) Important for permeability to glycerol; mutation to A: Strongly decreased permeability to glycerol. Mildly decreased water permeability.
H165 (≠ V127) mutation to A: Decreased permeability to glycerol. Mildly decreased water permeability.
G264 (= G230) to V: affects water and glycerol transport; dbSNP:rs62542743

Sites not aligning to the query:

1:32 mutation Missing: Decreased interaction with PLIN1.
12 R → C: in dbSNP:rs139297434

6n1gA Crystal structure of aquaglyceroporin aqp7 (see paper)
32% identity, 98% coverage: 2:239/243 of query aligns to 4:242/249 of 6n1gA

query
sites
6n1gA

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binding glycerol: L73 (≠ A74), R75 (≠ K76)

8c9hA Aqp7_inhibitor (see paper)
32% identity, 98% coverage: 2:239/243 of query aligns to 10:248/253 of 8c9hA

query
sites
8c9hA

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L

binding ethyl 4-[(4-pyrazol-1-ylphenyl)methylcarbamoylamino]benzoate: F49 (≠ W44), A66 (= A61), H67 (= H62), L157 (= L144), Q158 (≠ L145), F162 (= F149), I181 (≠ V173)

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
34% identity, 98% coverage: 4:242/243 of query aligns to 61:305/306 of I1CR68

query
sites
I1CR68

F

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A

G

S

L

I

V

A

G

A

L

V

M

L

L

-

M

-

P

-

F

-

S

Y

F

L

L

S

S

I

V

K

R

H275 (vs. gap) mutation to A: Affects pH sensing; when associated with A-85.

Q96PS8 Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin from Homo sapiens (Human) (see 2 papers)
32% identity, 96% coverage: 5:238/243 of query aligns to 25:260/301 of Q96PS8

query
sites
Q96PS8

V

L

A

A

E
|
E

L

F

I

L

G

G

T

V

M

F

L

V

I

L

I

M

L

L

G

T

D

Q

G

G

V

A

V

V

A

A

N

Q

V

A

V

V

L

T

K

S

-

G

D

E

T

T

K

K

G

G

N

N

N

-

G

-

W

F

I

F

V

T

I

M

T

F

T

L

A

A

W

G

G

S

L

L

A

A

V

V

F

T

V

I

G

A

V

I

V

Y

V

V

A
x
G

G

G

P

N

Y

V

S
|
S

G

G

A

A

H
|
H

L

L

N

N

P

P

A

A

V
x
F

T

S

L

L

A

A

L

M

A

C

I

I

A

V

G

G

K
x
R

F

L

A

P

W

W

A

V

N

K

V

L

I

P

P

I

Y

Y

I

I

I

L

A

V

Q

Q

F

L

A

L

G

S

A

A

C

F

I

C

G

A

A

S

F

G

L

A

V

T

W

Y

I

V

M

L

Y

Y

Y

H

D

D

H

A

F

L

K

Q

R

N

-

Y

-

T

-

G

-
x
N

-

L

-

T

T

V

N

T

D

G

P

P

A

K

S

E

I

T

L

A

A

S

V

I

F

F

C

A

T

T

G

Y

P

P

A

A

V

P

R

Y

N

L

Y

S

V

L

S

N

N

N

-

G

I

F

A

L

S

D

E

Q

V

V

I

L

G

G

A

T

F

G

V

M

L

L

L

I

F

V

T

G

I

L

F

L

Y

A

I

I

A

-

G

-

A

L

E

D

I

R

T

R

P

N

A

K

K

G

T

V

P

P

V

A

G

G

L

L

G

E

S

P

I

-

G

-

A

-

I

V

P

V

V

V

A

G

F

M

L

L

V

I

W

L

V

A

I

L

G

G

L

L

S

S

L

M

G

G

G

A

T

N

T

C

G

G

Y

I

A

P

I

L

N

N

P

P

A

A

R

R

D

D

L

L

G

G

P

P

R

R

F

L

M

F

H

T

M

Y

L

V

L

A

-

G

-

W

-

G

-

P

P

E

I

V

K

F

G

S

K

A

G

G

T

N

S

G

D

W

W

W

A

-

Y

-

A

-

W

W

I

V

P

P

V

V

I

V

A

A

P

P

L

L

V

V

G

G

A

A

S

T

I

V

A

G

A

T

V

A

L

T

Y

Y

E27 (= E7) mutation to Q: Abolishes permeability to glycerol.
G73 (≠ A55) mutation to A: Increased permeability to glycerol at acidic pH.; mutation to F: Abolishes permeability to glycerol.
S77 (= S59) mutation S->A,D: Nearly abolishes permeability to glycerol.
H80 (= H62) mutation to A: Abolishes permeability to glycerol.
F85 (≠ V67) mutation to A: Nearly abolishes permeability to glycerol.
R94 (≠ K76) mutation to A: Abolishes permeability to glycerol.
N133 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Abolishes N-glycosylation.

6f7hC Crystal structure of human aqp10 (see paper)
32% identity, 96% coverage: 5:238/243 of query aligns to 10:245/253 of 6f7hC

query
sites
6f7hC

V

L

A

A

E

E

L

F

I

L

G

G

T

V

M

F

L

V

I

L

I

M

L

L

G

T

D

Q

G

G

V

A

V

V

A

A

N

Q

V

A

V

V

L

T

K

S

-

G

D

E

T

T

K

K

G

G

N

N

N

-

G

-

W

F

I

F

V

T

I

M

T

F

T

L

A

A

W

G

G

S

L

L

A

A

V

V

F

T

V

I

G

A

V
x
I

V

Y

V

V

A

G

G

G

P

N

Y

V

S

S

G

G

A

A

H

H

L

L

N

N

P

P

A

A

V

F

T

S

L

L

A

A

L

M

A

C

I

I

A

V

G

G

K

R

F

L

A

P

W

W

A

V

N

K

V

L

I

P

P

I

Y

Y

I

I

I

L

A

V

Q

Q

F

L

A

L

G

S

A

A

C

F

I

C

G

A

A

S

F

G

L

A

V

T

W

Y

I

V

M

L

Y

Y

Y

H

D

D

H

A

F

L

K

Q

R

N

-

Y

-

T

-

G

-

N

-

L

-

T

T

V

N

T

D

G

P

P

A

K

S

E

I

T

L

A

A

S

V

I

F

F

C

A

T

T

G

Y

P

P

A

A

V

P

R

Y

N

L

Y

S

V

L

S

N

N

N

-

G

I

F

A

L

S

D

E

Q

V

V

I

L

G

G

A

T

F

G

V

M

L

L

L

I

F

V

T

G

I

L

F

L

Y

A

I

I

A

-

G

-

A

L

E

D

I

R

T

R

P

N

A

K

K

G

T

V

P

P

V

A

G

G

L

L

G

E

S

P

I

-

G

-

A

-

I

V

P

V

V

V

A

G

F

M

L

L

V

I

W

L

V

A

I

L

G

G

L

L

S

S

L

M

G

G

G

A

T

N

T

C

G

G

Y

I

A

P

I

L

N
|
N

P

P

A

A

R

R

D

D

L

L

G

G

P

P

R

R

F

L

M

F

H

T

M

Y

L

V

L

A

-

G

-

W

-

G

-

P

P

E

I

V

K

F

G

S

K

A

G

G

T

N

S

G

D

W

W

W

A

-

Y

-

A

-

W

W

I

V

P

P

V

V

I

V

A

A

P

P

L

L

V

V

G

G

A

A

S

T

I

V

A

G

A

T

V

A

L

T

Y

Y

binding glycerol: I55 (≠ V52), N199 (= N193)

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 96% coverage: 6:238/243 of query aligns to 11:249/264 of P37451

query
sites
P37451

A

A

E

E

L

F

I

L

G

G

T

T

M

G

L

L

I

F

I

L

L

F

G

G

D

I

G

G

V

C

V

L

A

S

N

-

V

-

V

A

L

L

K

K

D

V

T

A

K

G

G

A

N

S

N

L

G

G

G

L

W

W

I

-

V

E

I

I

T

C

T

I

A

I

W

W

G

G

L

L

A

G

V

I

F

S

V

L

G

A

V

V

V

Y

V

L

A

T

G

A

P

G

Y

I

S

S

G

G

A

G

H

H

L

L

N
|
N

P
|
P

A
|
A

V

V

T

T

L

I

A

A

L

L

A

W

I

L

A

F

G

A

K

C

F

F

A

P

W

K

A

Q

N

K

V

V

I

L

P

P

Y

Y

I

I

A

A

Q

Q

F

F

A

A

G

G

A

A

C

F

I

G

G

G

A

A

F

L

L

L

V

A

W

Y

I

V

M

L

Y

Y

Y

S

D

S

H

L

F

F

-

T

-

E

-

F

-

E

-

T

-

A

-

H

-

M

-

V

K

R

R

G

T

S

N

V

D

E

P

S

A

L

S

Q

I

L

L

A

A

S

V

I

F

F

C

S

T

T

G

Y

P

P

A

A

V

A

R

A

N

L

Y

N

V

V

S

W

N

Q

I

A

A

A

-

L

S

V

E

E

V

V

I

V

G

I

A

T

F

S

V

I

L

L

L

M

F

G

T

M

I

I

F

M

Y

-

I

-

A

-

G

-

A

-

E

A

I

L

T

T

P

D

A

D

K

G

T

N

P

G

V

I

G

P

L

K

G

G

S

P

I

L

G

A

A

P

I

L

P

L

V

I

A

G

F

I

L

L

V

V

W

A

V

V

I

I

G

G

L

A

S

S

L

T

G

G

G

P

T

L

T

T

G

G

Y

F

A

A

I

M

N
|
N

P
|
P

A
|
A

R

R

D

D

L

F

G

G

P

P

R

K

F

L

M

F

H

T

M

W

L

L

L

A

-

G

-

W

-

G

P

N

I

M

K

A

G

M

K

S

G

G

T

G

S

R

D

E

W

I

A

P

Y

Y

A

F

W

I

I

V

P

P

V

I

I

V

A

A

P

P

L

V

V

I

G

G

A

A

S

C

I

A

A

G

A

A

V

A

L

I

Y

Y

NPA 66:68 (= NPA 64:66) NPA 1
NPA 201:203 (= NPA 193:195) NPA 2

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
33% identity, 96% coverage: 5:238/243 of query aligns to 10:249/269 of B1VB61

query
sites
B1VB61

V

I

A

A

E

E

L

F

I

L

G

G

T

T

M

G

L

L

I

F

I

L

L

F

G

G

D

I

G

G

V

C

V

L

A

S

N

-

V

-

V

A

L

L

K

K

D

V

T

A

K

G

G

A

N

S

N

L

G

G

G

L

W

W

I

-

V

E

I

I

T

C

T

I

A

I

W

W

G

G

L

L

A

G

V

I

F

S

V

L

G

A

V

V

V

Y

V

L

A

T

G

A

P

G

Y

I

S

S

G

G

A

A

H

H

L

L

N
|
N

P
|
P

A
|
A

V

I

T

T

L

I

A

A

L

L

A

W

I

L

A

F

G

A

K

C

F

F

A

P

W

G

A

R

N

K

V

V

I

L

P

P

Y

Y

I

T

I

V

A

A

Q

Q

F

V

A

A

G

G

A

A

C

F

I

G

G

G

A

A

F

L

L

L

V

A

W

Y

I

L

M

L

Y

Y

Y

G

D

S

H

L

F

F

K

T

R

E

T

Y

N

E

D

S

P

A

A

H

S

Q

I

M

L

V

-

R

-

G

-

S

-

L

-

E

-

S

-

L

-

H

-

L

-

A

A

S

V

I

F

F

C

S

T

T

G

Y

P

P

A

A

V

A

R

A

N

L

Y

S

V

V

S

W

N

Q

I

A

A

A

-

L

S

V

E

E

V

V

I

V

G

I

A

T

F

S

V

I

L

L

L

M

F

G

T

M

I

I

F

M

Y

-

I

-

A

-

G

-

A

-

E

A

I

L

T

T

P

D

A

D

K

G

T

N

P

G

V

V

G

P

L

K

G

G

S

P

I

L

G

A

A

P

I

L

P

L

V

I

A

G

F

I

L

L

V

V

W

A

V

V

I

I

G

G

L

A

S

S

L

T

G

G

G

P

T

L

T

T

G

G

Y

F

A

A

I

M

N
|
N

P
|
P

A
|
A

R

R

D

D

L

F

G

G

P

P

R

K

F

L

M

F

H

A

M

W

L

M

L

A

-

G

-

W

-

G

P

D

I

V

K

A

G

M

K

T

G

G

T

G

S

R

D

D

W

I

A

P

Y

Y

A

F

W

I

I

V

P

P

V

I

I

V

A

A

P

P

L

I

V

I

G

G

A

A

S

C

I

A

A

G

A

A

V

A

L

I

Y

Y

NPA 66:68 (= NPA 64:66) NPA 1
NPA 201:203 (= NPA 193:195) NPA 2

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
33% identity, 96% coverage: 5:238/243 of query aligns to 9:240/245 of 2evuA

query
sites
2evuA

V

I

A

A

E

E

L

F

I

I

G

G

T

T

M

F

L

I

I

L

I

V

L

F

G

G

D

A

G

G

V

S

V

A

A

A

N

V

V

T

V

L

L

M

K

I

D

A

T

S

K

G

G

G

N

T

N

S

-

P

-

N

-

P

-

F

-

N

-
x
I

-

G

-

I

-

G

-

L

-

G

-

L

G

G

G

D

W
|
W

I

V

V

A

I

I

T

G

T

L

A

A

W

F

G

G

L

F

A

A

V
x
I

F

A

V

A

G

S

V

I

V

Y

V

A

A

L

G

G

P

N

Y

I

S

S

G

G

A

C

H

H

L
x
I

N
|
N

P

P

A

A

V

V

T

T

L

I

A

G

L

L

A

W

I

S

A

V

G

K

K

K

F

F

A

P

W

G

A

R

N

E

V

V

I

V

P

P

Y

Y

I

I

A

A

Q

Q

F

L

A

L

G

G

A

A

C

A

I

F

G

G

A

S

F

F

L

I

V

-

W

-

I

-

M

-

Y

-

D

-

H

-

F

F

K

L

R

Q

T

C

N

A

-

G

-

I

D

G

P

A

A

A

S

T

I

V

L

G

A

G

V

L

F
x
G

C
x
A

T

T

G
x
A

P
|
P

A
x
F

V

P

R

G

-

I

N

S

Y

Y

V

W

S

Q

N

A

I

M

A

L

S

A

E

E

V

V

I

V

G

G

A

T

F

F

V

L

L

L

L

M

F

I

T

T

I

I

F

M

Y

G

I

I

A

A

G

V

A

D

E

E

I

-

T

-

P

-

A

-

K

R

T

A

P

P

V

K

G

G

L

F

G

-

S

-

I

-

G

A

A

G

I

I

P

I

V

I

A

G

F

L

L

T

V

V

W

A

V

G

I

I

G

I

L

T

S

T

L

L

G

G

G

N

T

I

T

S

G

G

Y

S

A

S

I

L

N

N

P

P

A

A

R

R

D

T

L

F

G

G

P

P

R

Y

F

L

M

N

H

D

M

M

L

I

L

F

P

-

I

-

K

-

G

-

K

A

G

G

T

T

S

D

D

L

W

W

A

N

Y

Y

A

Y

W

S

I

I

P

Y

V

V

I

I

A

G

P

P

L

I

V

V

G

G

A

A

S

V

I

L

A

A

V

L

L

T

Y

Y

binding glycerol: I44 (vs. gap), W55 (= W37), I66 (≠ V48), I81 (≠ L63), N82 (= N64), G133 (≠ F121), A134 (≠ C122), A136 (≠ G124), P137 (= P125), F138 (≠ A126)

P43286 Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 96% coverage: 5:238/243 of query aligns to 42:266/287 of P43286

query
sites
P43286

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Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial; K→A: 2-fold decrease in water transport activity.; K→R: No effect.
6 E→A: No effect.
280 modified: Phosphoserine; S→A: Normal subcellular localization.
283 modified: Phosphoserine; S→A: Intracellular reticulation pattern, probably corresponding to the endoplasmic reticulum.; S→D: Normal subcellular localization.

P43287 Aquaporin PIP2-2; Plasma membrane intrinsic protein 2-2; AtPIP2;2; Plasma membrane intrinsic protein 2b; PIP2b; TMP2b from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 96% coverage: 5:238/243 of query aligns to 40:264/285 of P43287

query
sites
P43287

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x
H

R194 (≠ A159) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
D195 (≠ K160) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
H197 (≠ P162) mutation to A: Reduced water transport activity. Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-194 or A-195.; mutation to D: Reduced water transport activity. Insensitive to cytosolic acidification.; mutation to K: Very low water transport activity. Insensitive to cytosolic acidification.
H264 (≠ Y238) mutation to A: No effect.

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial

P30302 Aquaporin PIP2-3; Plasma membrane intrinsic protein 2-3; AtPIP2;3; Plasma membrane intrinsic protein 2c; PIP2c; RD28-PIP; TMP2C; Water stress-induced tonoplast intrinsic protein; WSI-TIP from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 96% coverage: 5:238/243 of query aligns to 40:264/285 of P30302

query
sites
P30302

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H

T223 (≠ Y190) mutation to C: Creates some mercury-sensitivity.

P61837 Aquaporin PIP1-1; AtPIP1;1; Plasma membrane aquaporin-1; Plasma membrane intrinsic protein 1a; PIP1a from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 97% coverage: 5:239/243 of query aligns to 55:274/286 of P61837

query
sites
P61837

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Sites not aligning to the query:

1 modified: N-acetylmethionine

P55088 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Mus musculus (Mouse) (see 2 papers)
31% identity, 96% coverage: 6:238/243 of query aligns to 40:248/323 of P55088

query
sites
P55088

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G

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M

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G

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G

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N

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W

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W

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L

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Y

S111 (≠ A78) modified: Phosphoserine; by PKG; mutation to A: Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.

Sites not aligning to the query:

4 natural variant: G -> R

Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 97% coverage: 5:239/243 of query aligns to 55:274/286 of Q06611

query
sites
Q06611

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N

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A

-

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Sites not aligning to the query:

1 modified: N-acetylmethionine

Q08733 Aquaporin PIP1-3; AtPIP1;3; Plasma membrane intrinsic protein 1c; PIP1c; Transmembrane protein B; TMP-B from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 96% coverage: 5:238/243 of query aligns to 55:273/286 of Q08733

query
sites
Q08733

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-

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T

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N

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Y

M

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L

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Y

D

A

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A

A

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L

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Y

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H

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q9XF58 Aquaporin PIP2-5; Plasma membrane intrinsic protein 2-5; ZmPIP2-5; ZmPIP2;5; ZmPIP2a from Zea mays (Maize) (see paper)
29% identity, 96% coverage: 5:238/243 of query aligns to 40:266/285 of Q9XF58

query
sites
Q9XF58

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A
x
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