SitesBLAST
Comparing WP_091292217.1 NCBI__GCF_900107045.1:WP_091292217.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
37% identity, 92% coverage: 3:239/257 of query aligns to 2:237/501 of P04983
- K43 (= K44) mutation to R: Loss of transport.
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
33% identity, 88% coverage: 4:230/257 of query aligns to 1:223/241 of 4u00A
3c4jA Abc protein artp in complex with atp-gamma-s
31% identity, 88% coverage: 5:230/257 of query aligns to 3:225/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
31% identity, 88% coverage: 5:230/257 of query aligns to 3:225/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
31% identity, 88% coverage: 5:230/257 of query aligns to 3:225/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
31% identity, 88% coverage: 5:230/257 of query aligns to 3:225/242 of 2oljA
Q9AT00 Protein TRIGALACTOSYLDIACYLGLYCEROL 3, chloroplastic; ABC transporter I family member 13; ABC transporter ABCI.13; AtABCI13; Non-intrinsic ABC protein 11; AtNAP11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 89% coverage: 2:231/257 of query aligns to 81:324/345 of Q9AT00
- F94 (= F15) mutation to A: Reduced ATPase activity and transport properties.
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
32% identity, 86% coverage: 13:232/257 of query aligns to 9:225/240 of 4ymuJ
- binding adenosine-5'-triphosphate: F11 (= F15), V16 (= V20), S36 (≠ N40), G37 (= G41), S38 (≠ A42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E169), H194 (= H201)
- binding magnesium ion: S41 (= S45), E162 (= E169)
5x40A Structure of a cbio dimer bound with amppcp (see paper)
37% identity, 86% coverage: 4:224/257 of query aligns to 3:221/280 of 5x40A
- binding phosphomethylphosphonic acid adenylate ester: F14 (= F15), V18 (= V18), A20 (≠ V20), N40 (= N40), G41 (= G41), G43 (= G43), K44 (= K44), S45 (= S45), T46 (= T46), Q88 (= Q87), H139 (≠ S142), M140 (≠ A143), L141 (= L144), S142 (= S145), G144 (= G147), Q145 (= Q148), Q166 (≠ E169), H198 (= H201)
- binding magnesium ion: S45 (= S45), Q88 (= Q87)
1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
27% identity, 86% coverage: 5:224/257 of query aligns to 4:231/254 of 1g6hA
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
27% identity, 86% coverage: 5:224/257 of query aligns to 4:231/253 of 1g9xB
6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
27% identity, 93% coverage: 5:242/257 of query aligns to 2:235/240 of 6mjpA
1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
26% identity, 97% coverage: 1:250/257 of query aligns to 2:240/240 of 1ji0A
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 96% coverage: 4:249/257 of query aligns to 16:253/378 of P69874
- C26 (≠ S14) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F15) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ V33) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ A42) mutation to T: Loss of ATPase activity and transport.
- L60 (≠ V48) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ F64) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ L129) mutation to M: Loss of ATPase activity and transport.
- D172 (= D168) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
34% identity, 86% coverage: 6:225/257 of query aligns to 4:215/371 of P68187
- A85 (= A90) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ S109) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ S117) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ Q120) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ A122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G147) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D168) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
34% identity, 86% coverage: 6:225/257 of query aligns to 3:214/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F15), S37 (≠ N40), G38 (= G41), C39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), Q81 (= Q87), R128 (= R132), A132 (= A143), S134 (= S145), G136 (= G147), Q137 (= Q148), E158 (= E169), H191 (= H201)
- binding magnesium ion: S42 (= S45), Q81 (= Q87)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
34% identity, 86% coverage: 6:225/257 of query aligns to 3:214/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F15), G38 (= G41), C39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), R128 (= R132), S134 (= S145), Q137 (= Q148)
- binding beryllium trifluoride ion: S37 (≠ N40), G38 (= G41), K41 (= K44), Q81 (= Q87), S134 (= S145), G136 (= G147), H191 (= H201)
- binding magnesium ion: S42 (= S45), Q81 (= Q87)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
34% identity, 86% coverage: 6:225/257 of query aligns to 3:214/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F15), V17 (= V20), G38 (= G41), C39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), R128 (= R132), A132 (= A143), S134 (= S145), Q137 (= Q148)
- binding tetrafluoroaluminate ion: S37 (≠ N40), G38 (= G41), K41 (= K44), Q81 (= Q87), S134 (= S145), G135 (= G146), G136 (= G147), E158 (= E169), H191 (= H201)
- binding magnesium ion: S42 (= S45), Q81 (= Q87)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
34% identity, 86% coverage: 6:225/257 of query aligns to 3:214/371 of 3puvA