SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_091292974.1 NCBI__GCF_900107045.1:WP_091292974.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 84% coverage: 23:258/280 of query aligns to 26:261/265 of P07821

query
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P07821

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K50 (= K47) mutation to Q: Lack of activity.
D172 (= D169) mutation to E: Lack of activity.
E173 (= E170) mutation to A: Lack of activity.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
35% identity, 80% coverage: 9:233/280 of query aligns to 2:224/240 of 4ymuJ

query
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4ymuJ

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binding adenosine-5'-triphosphate: F11 (≠ Y18), V16 (= V23), S36 (≠ N43), G37 (≠ A44), S38 (≠ C45), G39 (= G46), K40 (= K47), S41 (= S48), T42 (= T49), E162 (= E170), H194 (= H203)
binding magnesium ion: S41 (= S48), E162 (= E170)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
34% identity, 75% coverage: 24:234/280 of query aligns to 42:252/382 of 7ahhC

query
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7ahhC

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binding phosphoaminophosphonic acid-adenylate ester: G62 (≠ A44), G64 (= G46), K65 (= K47), D187 (= D169), E188 (= E170)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
34% identity, 75% coverage: 24:234/280 of query aligns to 42:252/382 of 7aheC

query
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7aheC

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Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
34% identity, 75% coverage: 24:234/280 of query aligns to 42:252/260 of 7ahdC

query
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7ahdC

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binding adenosine-5'-triphosphate: S61 (≠ N43), G62 (≠ A44), G64 (= G46), K65 (= K47), S66 (= S48), T67 (= T49), Q111 (= Q89), K161 (≠ D143), Q162 (≠ E144), S164 (= S146), G166 (= G148), M167 (≠ Q149), Q188 (≠ E170), H221 (= H203)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
33% identity, 81% coverage: 7:234/280 of query aligns to 1:225/241 of 4u00A

query
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4u00A

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G

A

E

V

V

F

V

L

V

D

D

G

G

K

L

G

S

I

V

T

K

T

D

L

D

R

R

S

A

-

L

K

R

D

E

V

I

A

R

T

R

V

E

L

V

G

G

M

M

L

V

P

F

Q

Q

S

Q

-

F

-

N

-

L

-

F

-

P

-

H

P

M

T

T

A

V

P

L

E

E

G

N

I

V

T

T

V

L

A

A

D

P

L

M

V

-

G

-

R

-

G

-

R

-

H

-

P

-

H

-

Q

-

G

-

M

R

F

V

R

R

W

W

S

P

E

R

E

E

D

K

D

A

A

E

A

K

V

K

A

A

R

L

A

E

M

L

L

L

A

E

-

R

T

V

D

G

T

I

V

L

Q

D

L

Q

A

A

S

R

R

K

P

Y

V

P

D

A

E

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

Q

E

T

P

P

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

F

A

L

L

D

D

I

P

A

E

H

M

Q

V

V

G

E

E

V

V

L

L

D

D

L

V

L

M

A

R

E

D

L

L

N

-

A

A

A

Q

D

G

G

G

R

M

T

T

I

M

V

V

I

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

R

E

Y

V

G

A

D

D

H

R

L

V

I

V

A

F

M

M

K

D

D

G

G

G

R

Q

I

I

A

V

A

E

E

E

G

G

A

R

P

P

A

E

E

E

I

I

L

F

T

T

binding adenosine-5'-diphosphate: F12 (≠ Y18), V17 (= V23), S37 (≠ N43), G38 (≠ A44), S39 (≠ C45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
33% identity, 77% coverage: 11:226/280 of query aligns to 8:226/232 of 1f3oA

query
sites
1f3oA

T

T

E

K

D

T

L
x
Y

E

K

L

M

G

G

Y

E

G

E

P

I

L

I

R

Y

V

A

V

L

K

K

D

N

L

V

S

N

V

L

R

N

I

I

P

K

D

E

G

G

K

E

V

F

T

V

M

S

I

I

V

M

G

G

T

P

N

S

A
x
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

G

I

L

I

A

G

R

C

L

L

L

D

T

K

P

P

T

T

A

E

G

G

A

E

V

V

F

Y

-

I

-

D

-

N

-

I

-

K

-

T

-

N

-

D

L

L

D

D

G

D

K

D

G

E

I

L

T

T

T

K

L

I

R

R

S

R

K

D

D

K

V

I

A

G

T

F

V

V

L

F

G

Q

M

Q

L

F

P

N

Q

L

S

I

P

P

-

L

T

T

A

A

P

L

E

E

G

N

I

V

T

E

V

L

A

P

D

-

L

L

V

I

G

F

R

K

G

Y

R

R

H

G

P

A

H

M

Q

S

G

G

M

E

F

E

R

R

W

K

S

R

E

-

D

-

A

-

A

A

V

L

A

E

R

C

A

L

M

K

L

M

A

A

T

E

D

L

T

E

V

E

Q

R

L

F

A

A

S

N

R

H

P

K

V

P

D

N

E

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

N

T

P

P

P

L

I

L

L

L

A

D

D

E

E

P

P

T

T

T

G

F

A

L

L

D

D

I

S

A

K

H

T

Q

G

V

E

E

K

V

I

L

M

D

Q

L

L

A

K

E

K

L

L

N

N

A

E

D

D

G

G

R

K

T

T

I

V

V

V

I

V

V

V

L

T

H

H

D

D

L

I

N

N

L

V

A

-

A

A

R

R

Y

F

G

G

D

E

H

R

L

I

I

I

A

Y

M

L

K

K

D

D

G

G

R

E

I

V

A

E

A

R

E

E

binding adenosine-5'-diphosphate: Y11 (≠ L14), G41 (≠ A44), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
33% identity, 77% coverage: 11:226/280 of query aligns to 8:226/230 of 1l2tA

query
sites
1l2tA

T

T

E

K

D

T

L
x
Y

E

K

L

M

G

G

Y

E

G

E

P

I

L

I

R

Y

V

A

V

L

K

K

D

N

L

V

S

N

V

L

R

N

I

I

P

K

D

E

G

G

K

E

V

F

T

V

M

S

I

I

V

M

G

G

T

P

N
x
S

A
x
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

G

I

L

I

A

G

R

C

L

L

L

D

T

K

P

P

T

T

A

E

G

G

A

E

V

V

F

Y

-

I

-

D

-

N

-

I

-

K

-

T

-

N

-

D

L

L

D

D

G

D

K

D

G

E

I

L

T

T

T

K

L

I

R

R

S

R

K

D

D

K

V

I

A

G

T

F

V

V

L

F

G

Q

M

Q

L

F

P

N

Q

L

S

I

P

P

-

L

T

T

A

A

P

L

E

E

G

N

I

V

T

E

V

L

A

P

D

-

L

L

V

I

G

F

R

K

G

Y

R

R

H

G

P

A

H

M

Q

S

G

G

M

E

F

E

R

R

W

K

S

R

E

-

D

-

A

-

A

A

V

L

A

E

R

C

A

L

M

K

L

M

A

A

T

E

D

L

T

E

V

E

Q

R

L
x
F

A

A

S

N

R

H

P

K

V

P

D

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

Q

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

N

T

P

P

P

L

I

L

L

L

A

D

D

E

Q

P

P

T

T

T

G

F

A

L

L

D

D

I

S

A

K

H

T

Q

G

V

E

E

K

V

I

L

M

D

Q

L

L

A

K

E

K

L

L

N

N

A

E

D

D

G

G

R

K

T

T

I

V

V

V

I

V

V

V

L

T

H
|
H

D

D

L

I

N

N

L

V

A

-

A

A

R

R

Y

F

G

G

D

E

H

R

L

I

I

I

A

Y

M

L

K

K

D

D

G

G

R

E

I

V

A

E

A

R

E

E

binding adenosine-5'-triphosphate: Y11 (≠ L14), S40 (≠ N43), G41 (≠ A44), S42 (≠ C45), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49), F138 (≠ L137), Q145 (≠ E144), S147 (= S146), G149 (= G148), Q150 (= Q149), H204 (= H203)

3c4jA Abc protein artp in complex with atp-gamma-s
36% identity, 79% coverage: 14:233/280 of query aligns to 9:226/242 of 3c4jA

query
sites
3c4jA

L

L

E

K

L

K

G

S

Y
x
F

G

G

P

S

L

L

R

E

V
|
V

V

L

K

K

D

G

L

I

S

N

V

V

R

H

I

I

P

R

D

E

G

G

K

E

V

V

T

V

M

V

I

V

V

I

G

G

T

P

N
x
S

A
x
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

G

C

L

L

A

N

R

L

L

L

L

E

T

D

P

F

T

D

A

E

G

G

A

E

V

I

F

I

L

I

D

D

G

G

K

-

G

-

I

-

T

I

T

N

L

L

R

K

S

A

K

K

D

D

-

T

-

N

-

L

-

N

-

K

V

V

A

R

T

E

V

E

L

V

G

G

M

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

I

M

T

T

V

V

A

L

D

N

L

N

V

I

G

T

R

L

G

A

R

-

H

-

P

P

H

M

Q

K

G

-

M

-

F

V

R

R

R

K

W

W

S

P

E

R

E

E

D

-

A

K

A

A

V

E

A

A

R

K

A

A

M

M

L

E

A

L

T

L

D

D

T

K

V

V

Q

G

L

L

A

K

S

D

R

K

P

A

V

H

-

A

-

Y

-

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

Q

E

T

P

P

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

F

A

L

L

D

D

I

P

A

E

H

M

Q

V

V

G

E

E

V

V

L

L

D

S

L

V

L

M

A

K

E

Q

L

L

N

-

A

A

A

N

D

E

G

G

R

M

T

T

I

M

V

V

I

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

R

E

Y

V

G

G

D

D

H

R

L

V

I

L

A

F

M

M

K

D

D

G

G

G

R

Y

I

I

A

I

A

E

E

E

G

G

A

K

P

P

A

E

E

D

I

L

L

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y18), V18 (= V23), S38 (≠ N43), G39 (≠ A44), S40 (≠ C45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)

3c41J Abc protein artp in complex with amp-pnp/mg2+
36% identity, 79% coverage: 14:233/280 of query aligns to 9:226/242 of 3c41J

query
sites
3c41J

L

L

E

K

L

K

G

S

Y
x
F

G

G

P

S

L

L

R

E

V
|
V

V

L

K

K

D

G

L

I

S

N

V

V

R

H

I

I

P

R

D

E

G

G

K

E

V

V

T

V

M

V

I

V

V

I

G

G

T

P

N
x
S

A
x
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

G

C

L

L

A

N

R

L

L

L

L

E

T

D

P

F

T

D

A

E

G

G

A

E

V

I

F

I

L

I

D

D

G

G

K

-

G

-

I

-

T

I

T

N

L

L

R

K

S

A

K

K

D

D

-

T

-

N

-

L

-

N

-

K

V

V

A

R

T

E

V

E

L

V

G

G

M

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

I

M

T

T

V

V

A

L

D

N

L

N

V

I

G

T

R

L

G

A

R

-

H

-

P

P

H

M

Q

K

G

-

M

-

F

V

R

R

R

K

W

W

S

P

E

R

E

E

D

-

A

K

A

A

V

E

A

A

R

K

A

A

M

M

L

E

A

L

T

L

D

D

T

K

V

V

Q

G

L

L

A

K

S

D

R

K

P

A

V

H

-

A

-

Y

-

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

Q

E

T

P

P

K

L

I

L

M

L

L

L

F

D
|
D

E

E

P

P

T

T

T

S

F

A

L

L

D

D

I

P

A

E

H

M

Q

V

V

G

E

E

V

V

L

L

D

S

L

V

L

M

A

K

E

Q

L

L

N

-

A

A

A

N

D

E

G

G

R

M

T

T

I

M

V

V

I

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

R

E

Y

V

G

G

D

D

H

R

L

V

I

L

A

F

M

M

K

D

D

G

G

G

R

Y

I

I

A

I

A

E

E

E

G

G

A

K

P

P

A

E

E

D

I

L

L

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y18), V18 (= V23), S38 (≠ N43), G39 (≠ A44), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)
binding magnesium ion: S43 (= S48), D163 (= D169)

2olkA Abc protein artp in complex with adp-beta-s
36% identity, 79% coverage: 14:233/280 of query aligns to 9:226/242 of 2olkA

query
sites
2olkA

L

L

E

K

L

K

G

S

Y
x
F

G

G

P

S

L

L

R

E

V
|
V

V

L

K

K

D

G

L

I

S

N

V

V

R

H

I

I

P

R

D

E

G

G

K

E

V

V

T

V

M

V

I

V

V

I

G

G

T

P

N
x
S

A
x
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

G

C

L

L

A

N

R

L

L

L

L

E

T

D

P

F

T

D

A

E

G

G

A

E

V

I

F

I

L

I

D

D

G

G

K

-

G

-

I

-

T

I

T

N

L

L

R

K

S

A

K

K

D

D

-

T

-

N

-

L

-

N

-

K

V

V

A

R

T

E

V

E

L

V

G

G

M

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

I

M

T

T

V

V

A

L

D

N

L

N

V

I

G

T

R

L

G

A

R

-

H

-

P

P

H

M

Q

K

G

-

M

-

F

V

R

R

R

K

W

W

S

P

E

R

E

E

D

-

A

K

A

A

V

E

A

A

R

K

A

A

M

M

L

E

A

L

T

L

D

D

T

K

V

V

Q

G

L

L

A

K

S

D

R

K

P

A

V

H

-

A

-

Y

-

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

Q

E

T

P

P

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

F

A

L

L

D

D

I

P

A

E

H

M

Q

V

V

G

E

E

V

V

L

L

D

S

L

V

L

M

A

K

E

Q

L

L

N

-

A

A

A

N

D

E

G

G

R

M

T

T

I

M

V

V

I

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

R

E

Y

V

G

G

D

D

H

R

L

V

I

L

A

F

M

M

K

D

D

G

G

G

R

Y

I

I

A

I

A

E

E

E

G

G

A

K

P

P

A

E

E

D

I

L

L

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y18), V18 (= V23), S38 (≠ N43), G39 (≠ A44), S40 (≠ C45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)

2oljA Abc protein artp in complex with adp/mg2+
36% identity, 79% coverage: 14:233/280 of query aligns to 9:226/242 of 2oljA

query
sites
2oljA

L

L

E

K

L

K

G

S

Y
x
F

G

G

P

S

L

L

R

E

V
|
V

V

L

K

K

D

G

L

I

S

N

V

V

R

H

I

I

P

R

D

E

G

G

K

E

V

V

T

V

M

V

I

V

V

I

G

G

T

P

N
x
S

A
x
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

G

C

L

L

A

N

R

L

L

L

L

E

T

D

P

F

T

D

A

E

G

G

A

E

V

I

F

I

L

I

D

D

G

G

K

-

G

-

I

-

T

I

T

N

L

L

R

K

S

A

K

K

D

D

-

T

-

N

-

L

-

N

-

K

V

V

A

R

T

E

V

E

L

V

G

G

M

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

I

M

T

T

V

V

A

L

D

N

L

N

V

I

G

T

R

L

G

A

R

-

H

-

P

P

H

M

Q

K

G

-

M

-

F

V

R

R

R

K

W

W

S

P

E

R

E

E

D

-

A

K

A

A

V

E

A

A

R

K

A

A

M

M

L

E

A

L

T

L

D

D

T

K

V

V

Q

G

L

L

A

K

S

D

R

K

P

A

V

H

-

A

-

Y

-

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

Q

E

T

P

P

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

F

A

L

L

D

D

I

P

A

E

H

M

Q

V

V

G

E

E

V

V

L

L

D

S

L

V

L

M

A

K

E

Q

L

L

N

-

A

A

A

N

D

E

G

G

R

M

T

T

I

M

V

V

I

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

R

E

Y

V

G

G

D

D

H

R

L

V

I

L

A

F

M

M

K

D

D

G

G

G

R

Y

I

I

A

I

A

E

E

E

G

G

A

K

P

P

A

E

E

D

I

L

L

F

binding adenosine-5'-diphosphate: F13 (≠ Y18), V18 (= V23), S38 (≠ N43), G39 (≠ A44), S40 (≠ C45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)

P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 85% coverage: 9:245/280 of query aligns to 7:244/258 of P02915

query
sites
P02915

L

L

H

H

T

V

E

I

D

D

L

L

E

H

L

K

G

R

Y

Y

G

G

P

G

L

H

R

E

V

V

V

L

K

K

D

G

L

V

S

S

V

L

R

Q

I

A

P

R

D

A

G

G

K

D

V

V

T

I

M

S

I

I

V

I

G

G

T

S

N
x
S

A
x
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

G

C

L

I

A

N

R

F

L

L

L

E

T

K

P

P

T

S

A

E

G

G

A

A

V

I

F

I

L

V

D

N

G

G

K

Q

G

N

I

I

T

N

T

L

L

V

R

R

S

D

K

K

D

D

-

G

-

Q

-

L

-

K

-

V

-

A

-

D

-

K

-

N

-

Q

-

L

-

R

-

L

V

L

A

R

T

T

V

R

L

L

G

T

M

M

L

V

P

F

Q

Q

S

H

P

F

T

N

A

L

P

W

E

S

G

H

I

M

T

T

V

V

A

L

D

E

L

N

V

V

G

M

R

E

G

A

R

-

H

-

P

P

H

I

Q

Q

G

V

M

L

-

G

F

L

R

S

R

K

W

H

S

D

E

A

E

R

D

E

D

R

A

A

A

L

V

K

A

Y

R

L

A

A

M

K

L

V

A

G

T

I

D

D

T

E

V

R

Q

A

L

Q

A

G

S

K

R

Y

P

P

V

V

D

-

E

H

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

W

S

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

Q

E

T

P

P

D

L

V

L

L

L

F

D

D

E

E

P

P

T

T

T

S

F

A

L

L

D

D

I

P

A

E

H

L

Q

V

V

G

E

E

V

V

L

L

D

R

L

I

L

M

A

Q

E

Q

L

L

N

-

A

A

A

E

D

E

G

G

R

K

T

T

I

M

V

V

I

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

R

H

Y

V

G

S

D

S

H

H

L

V

I

I

A

F

M

L

K

H

D

Q

G

G

R

K

I

I

A

E

E

E

G

G

A

D

P

P

A

-

E

-

I

-

L

-

T

-

A

-

E

-

L

-

V

-

E

E

E

Q

V

V

F

F

G

G

M

N

P

P

S41 (≠ N43) binding ATP
G42 (≠ A44) binding ATP
G44 (= G46) binding ATP
K45 (= K47) binding ATP
S46 (= S48) binding ATP
T47 (= T49) binding ATP

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
34% identity, 85% coverage: 8:245/280 of query aligns to 2:240/258 of 1b0uA

query
sites
1b0uA

T

K

L

L

H

H

T

V

E

I

D

D

L

L

E

H

L

K

G

R

Y
|
Y

G

G

P

G

L
x
H

R

E

V

V

V

L

K

K

D

G

L

V

S

S

V

L

R

Q

I

A

P

R

D

A

G

G

K

D

V

V

T

I

M

S

I

I

V

I

G

G

T

S

N
x
S

A
x
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

G

C

L

I

A

N

R

F

L

L

L

E

T

K

P

P

T

S

A

E

G

G

A

A

V

I

F

I

L

V

D

N

G

G

K

Q

G

N

I

I

T

N

T

L

L

V

R

R

S

D

K

K

D

D

-

G

-

Q

-

L

-

K

-

V

-

A

-

D

-

K

-

N

-

Q

-

L

-

R

-

L

V

L

A

R

T

T

V

R

L

L

G

T

M

M

L

V

P

F

Q

Q

S

H

P

F

T

N

A

L

P

W

E

S

G

H

I

M

T

T

V

V

A

L

D

E

L

N

V

V

G

M

R

E

G

A

R

-

H

-

P

P

H

I

Q

Q

G

V

M

L

-

G

F

L

R

S

R

K

W

H

S

D

E

A

E

R

D

E

D

R

A

A

A

L

V

K

A

Y

R

L

A

A

M

K

L

V

A

G

T

I

D

D

T

E

V

R

Q

A

L

Q

A

G

S

K

R

Y

P

P

V

V

D

-

E

H

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

W

S

I

I

A

A

M

R

A

A

L

L

A

A

Q

M

Q

E

T

P

P

D

L

V

L

L

L

F

D

D

E

E

P

P

T

T

T

S

F

A

L

L

D

D

I

P

A

E

H

L

Q

V

V

G

E

E

V

V

L

L

D

R

L

I

L

M

A

Q

E

Q

L

L

N

-

A

A

A

E

D

E

G

G

R

K

T

T

I

M

V

V

I

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

R

H

Y

V

G

S

D

S

H

H

L

V

I

I

A

F

M

L

K

H

D

Q

G

G

R

K

I

I

A

E

E

E

G

G

A

D

P

P

A

-

E

-

I

-

L

-

T

-

A

-

E

-

L

-

V

-

E

E

E

Q

V

V

F

F

G

G

M

N

P

P

binding adenosine-5'-triphosphate: Y12 (= Y18), H15 (≠ L21), S37 (≠ N43), G38 (≠ A44), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
35% identity, 73% coverage: 23:226/280 of query aligns to 24:226/233 of P75957

query
sites
P75957

V

V

V

L

K

H

D

N

L

V

S

S

V

F

R

S

I

V

P

G

D

E

G

G

K

E

V

M

T

M

M

A

I

I

V

V

G
|
G

T

S

N

S

A

G

C

S

G

G

K

K

S

S

T

T

L

L

R

H

G

L

L

L

A

G

R

G

L

L

L

D

T

T

P

P

T

T

A

S

G

G

A

D

V

V

F

I

L

F

D

N

G

G

K

Q

G

P

I

M

T

S

T

K

L

L

R

S

S

S

K

A

D

A

V

K

A

A

T

E

V

L

-

R

-

N

-

Q

-

K

L

L

G

G

M

F

L

I

P

Y

Q

Q

-

F

-

H

-

L

-

P

S

D

P

F

T

T

A

A

P

L

E

E

G

N

I

V

T

A

V

M

A

P

D

L

L

L

V

I

G

G

R

K

G

-

R

-

H

-

P

-

H

-

Q

-

G

-

M

-

F

-

R

-

R

K

W

K

S

P

E

A

E

E

D

I

D

N

A

S

A

R

V

A

A

L

R

E

A

M

M

L

L

K

A

A

T

V

D

G

T

L

V

D

Q

H

L

R

A

A

S

N

R

H

P

R

V

P

D

S

E

E

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

V

Q

N

T

P

P

R

L

L

L

V

L

L

L

A

D

D

E

E

P

P

T

T

T

G

F

N

L

L

D

D

I

A

A

R

H

N

Q

A

V

D

E

S

V

I

L

F

D

Q

L

L

A

G

E

E

L

L

N

N

A

R

A

L

D

Q

G

G

R

T

T

A

I

F

V

L

I

V

V

V

L

T

H

H

D

D

L

L

N

Q

L

L

A

A

A

K

R

R

Y

M

G

S

D

R

H

Q

L

L

I

-

A

E

M

M

K

R

D

D

G

G

R

R

I

L

A

T

A

A

E

E

G42 (= G41) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
35% identity, 73% coverage: 23:226/280 of query aligns to 21:223/226 of 7mdyC

query
sites
7mdyC

V

V

V

L

K

H

D

N

L

V

S

S

V

F

R

S

I

V

P

G

D

E

G

G

K

E

V

M

T

M

M

A

I

I

V

V

G

G

T

S

N

S

A
x
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

H

G

L

L

L

A

G

R

G

L

L

L

D

T

T

P

P

T

T

A

S

G

G

A

D

V

V

F

I

L

F

D

N

G

G

K

Q

G

P

I

M

T

S

T

K

L

L

R

S

S

S

K

A

D

A

V

K

A

A

T

E

V

L

-

R

-

N

-

Q

-

K

L

L

G

G

M

F

L

I

P

Y

Q
|
Q

-

F

-

H

-

L

-

P

S

D

P

F

T

T

A

A

P

L

E

E

G

N

I

V

T

A

V

M

A

P

D

L

L

L

V

I

G

G

R

K

G

-

R

-

H

-

P

-

H

-

Q

-

G

-

M

-

F

-

R

-

R

K

W

K

S

P

E

A

E

E

D

I

D

N

A

S

A

R

V

A

A

L

R

E

A

M

M

L

L

K

A

A

T

V

D

G

T

L

V

D

Q

H

L

R

A

A

S

N

R
x
H

P

R

V

P

D

S

E
|
E

L

L

S
|
S

G
|
G

Q

E

R

R

Q

Q

R

R

V

V

W

A

I

I

A

A

M

R

A

A

L

L

A

V

Q

N

T

P

P

R

L

L

L

V

L

L

L

A

D

D

E
|
E

P

P

T

T

T

G

F
x
N

L

L

D

D

I

A

A

R

H

N

Q

A

V

D

E

S

V

I

L

F

D

Q

L

L

A

G

E

E

L

L

N

N

A

R

A

L

D

Q

G

G

R

T

T

A

I

F

V

L

I

V

V

V

L

T

H
|
H

D

D

L

L

N

Q

L

L

A

A

A

K

R

R

Y

M

G

S

D

R

H

Q

L

L

I

-

A

E

M

M

K

R

D

D

G

G

R

R

I

L

A

T

A

A

E

E

binding adp orthovanadate: G42 (≠ A44), S43 (≠ C45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49), Q91 (= Q89), H138 (≠ R140), E142 (= E144), S144 (= S146), G145 (= G147), G146 (= G148), E168 (= E170), N172 (≠ F174), H201 (= H203)
binding magnesium ion: S46 (= S48), Q91 (= Q89)

Sites not aligning to the query:

binding adp orthovanadate: 12

P21439 Phosphatidylcholine translocator ABCB4; ATP-binding cassette sub-family B member 4; Multidrug resistance protein 3; P-glycoprotein 3; EC 7.6.2.1 from Homo sapiens (Human) (see 22 papers)
28% identity, 84% coverage: 4:238/280 of query aligns to 389:623/1286 of P21439

query
sites
P21439

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Y403 (= Y18) to H: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol; dbSNP:rs121918443
R406 (vs. gap) binding ATP; to Q: found in patients with cholangitis; uncertain significance; dbSNP:rs763807769
GCGKST 432:437 (≠ ACGKST 44:49) binding ATP
K435 (= K47) mutation to M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
E450 (≠ A62) to G: in dbSNP:rs1189003716
D459 (≠ G71) to H: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression
Q477 (= Q89) binding ATP
P479 (= P91) to L: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs748657435
L481 (≠ A93) to R: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol
A511 (= A123) to T: in PFIC3 and GBD1; dbSNP:rs1257887155
E528 (≠ R140) to D: in GBD1; uncertain significance; moderate decrease of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs8187797
G535 (= G147) to D: in PFIC3; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
G536 (= G148) binding ATP; to R: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location
I541 (≠ V153) to F: in PFIC3 and GBD1; dbSNP:rs66904256
A546 (= A158) to D: in ICP3; disruption of protein trafficking with subsequent lack of functional protein at the cell surface; dbSNP:rs121918441
E558 (= E170) mutation to Q: Loss of floppase activity. Strongly reduce the ATPase activity.
H589 (= H203) to T: in GBD1; requires 2 nucleotide substitutions
R590 (≠ D204) to Q: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs45575636

Sites not aligning to the query:

34 modified: Phosphothreonine; T → M: in GBD1; reduces efflux activity for PC in a phosphorylation-dependent manner; dbSNP:rs142794414; T→D: Does not inhibit efflux activity for PC.
44 T→A: Reduces efflux activity for PC. Does not alter apical membrane location.
47 R → G: in GBD1; partly retained intracellularly; reduces efflux activity for PC in a phosphorylation-dependent manner; R → Q: found in patients with cholangitis; uncertain significance; dbSNP:rs372685632
49 S→A: Reduces efflux activity for PC. Does not alter apical membrane location.
68 G → R: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression; dbSNP:rs1343667900
73 L → V: in PFIC3 and GBD1; dbSNP:rs8187788
87 natural variant: D -> E
95 P → S: in dbSNP:rs377268767
175 T → A: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs58238559
201 T → M: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs753318087
238 L → V: in dbSNP:rs45596335
263 I → V: in dbSNP:rs45547936
286 A → V: in PFIC3 and GBD1; does not alter plasma membrane location; inhibits efflux activity for PC; dbSNP:rs765478923
320 S → F: in ICP3, GBD1 and PFIC3; uncertain significance; does not alter plasma membrane location; does not inhibit efflux activity for PC; dbSNP:rs72552778
367 I → V: in dbSNP:rs1168923653
651 T → N: in dbSNP:rs45476795
652 R → G: in dbSNP:rs2230028
726 P → L: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs141677867
742 natural variant: G -> S
764 I → L: in a heterozygous patient with risperidone-induced cholestasis
775 T → M: found in patients with cholangitis; uncertain significance; dbSNP:rs148052192
788 R → Q: in GBD1; benign; dbSNP:rs8187801
934 A → T: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs61730509
953 A→D: Accumulates predominantly in intracellular compartments with only a small fraction at the plasma membrane and inhibits partially the efflux activity for PC.
964 V → T: found in patients with cholangitis; uncertain significance; requires 2 nucleotide substitutions
978 S → P: in PFIC3; alters efflux activity for PC; dbSNP:rs1051861187
985 V→M: Significantly reduces phosphatidylcholine floppase activity; when associated with Q-989 and V-990.
989 H→Q: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and V-990.
990 A→V: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and Q-989.
1046 binding ATP
1071:1077 binding ATP
1075 K→M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
1082 L → Q: in a heterozygous patient with amoxicillin/clavulanic acid-induced cholestasis; dbSNP:rs1214110864
1124 binding ATP
1125 E → K: in PFIC3; alters efflux activity for PC
1168 P → S: in GBD1; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs121918442
1183 S → L: in GBD1; severely reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
1184:1186 binding ATP
1185 G → S: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
35% identity, 73% coverage: 23:226/280 of query aligns to 23:225/229 of 7v8iD

query
sites
7v8iD

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binding phosphoaminophosphonic acid-adenylate ester: V23 (= V23), S43 (≠ N43), G44 (≠ A44), G46 (= G46), K47 (= K47), S48 (= S48), T49 (= T49), Q93 (= Q89), R137 (≠ L137), H140 (≠ R140), E144 (= E144), S146 (= S146), G148 (= G148), E149 (≠ Q149), H203 (= H203)
binding magnesium ion: S48 (= S48), Q93 (= Q89)

7otgA Structure of abcb1/p-glycoprotein in the presence of the cftr potentiator ivacaftor (see paper)
30% identity, 75% coverage: 23:233/280 of query aligns to 956:1165/1179 of 7otgA

query
sites
7otgA

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Sites not aligning to the query:

binding N-(2,4-di-tert-butyl-5-hydroxyphenyl)-4-oxo-1,4-dihydroquinoline-3-carboxamide: 307, 311, 779, 780

6q81A Structure of p-glycoprotein(abcb1) in the post-hydrolytic state (see paper)
30% identity, 75% coverage: 23:233/280 of query aligns to 959:1168/1182 of 6q81A

query
sites
6q81A

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