SitesBLAST
Comparing WP_091370263.1 NCBI__GCF_900105165.1:WP_091370263.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
40% identity, 83% coverage: 32:292/313 of query aligns to 20:283/304 of 1wwkA
- active site: S96 (≠ Y104), R230 (= R239), D254 (= D263), E259 (= E268), H278 (= H287)
- binding nicotinamide-adenine-dinucleotide: V100 (= V108), G146 (= G155), F147 (≠ L156), G148 (= G157), R149 (= R158), I150 (≠ V159), Y168 (≠ W177), D169 (≠ S178), P170 (≠ Q179), V201 (≠ L210), P202 (≠ V211), T207 (= T216), T228 (= T237), S229 (= S238), D254 (= D263), H278 (= H287), G280 (= G289)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
35% identity, 76% coverage: 56:292/313 of query aligns to 49:293/334 of 5aovA
- active site: L100 (vs. gap), R241 (= R239), D265 (= D263), E270 (= E268), H288 (= H287)
- binding glyoxylic acid: M52 (≠ V59), L53 (≠ M60), L53 (≠ M60), Y74 (≠ T83), A75 (≠ G84), V76 (≠ M85), G77 (≠ R86), R241 (= R239), H288 (= H287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ M85), T104 (≠ A112), F158 (≠ L156), G159 (= G157), R160 (= R158), I161 (≠ V159), S180 (= S178), R181 (≠ Q179), A211 (≠ H209), V212 (≠ L210), P213 (≠ V211), T218 (= T216), I239 (≠ T237), A240 (≠ S238), R241 (= R239), H288 (= H287), G290 (= G289)
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 46:292/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (vs. gap), A100 (= A112), R149 (= R158), I150 (≠ V159), Y168 (≠ W177), D169 (≠ S178), P170 (≠ Q179), I171 (≠ N180), H200 (= H209), T201 (≠ L210), P202 (≠ V211), T207 (= T216), C228 (≠ T237), A229 (≠ S238), R230 (= R239), H277 (= H287), G279 (= G289)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 47:293/301 of 6rj5A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 44:290/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G157), I148 (≠ V159), Y166 (≠ W177), D167 (≠ S178), P168 (≠ Q179), I169 (≠ N180), I170 (≠ L181), H198 (= H209), T199 (≠ L210), L208 (≠ I219), R228 (= R239)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 47:293/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (= L154), G147 (= G155), L148 (= L156), G149 (= G157), R150 (= R158), I151 (≠ V159), G152 (= G160), D170 (≠ S178), H201 (= H209), T202 (≠ L210), P203 (≠ V211)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 47:293/302 of 6rihA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 47:293/303 of 6plgA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 48:294/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 48:294/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ M85), A102 (= A112), G148 (= G155), R151 (= R158), I152 (≠ V159), Y170 (≠ W177), D171 (≠ S178), P172 (≠ Q179), I173 (≠ N180), H202 (= H209), T203 (≠ L210), P204 (≠ V211), T209 (= T216), C230 (≠ T237), A231 (≠ S238), R232 (= R239), H279 (= H287), G281 (= G289)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: E293 (= E301)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
31% identity, 78% coverage: 59:302/313 of query aligns to 46:292/297 of 6rj3A
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
31% identity, 78% coverage: 59:302/313 of query aligns to 52:298/533 of O43175
- T78 (≠ M85) binding NAD(+)
- R135 (≠ T140) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ RV 158:159) binding NAD(+)
- D175 (≠ S178) binding NAD(+)
- T207 (≠ L210) binding NAD(+)
- CAR 234:236 (≠ TSR 237:239) binding NAD(+)
- D260 (= D263) binding NAD(+)
- V261 (= V264) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIGY 287:290) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
7cvpA The crystal structure of human phgdh from biortus.
35% identity, 61% coverage: 112:302/313 of query aligns to 55:247/254 of 7cvpA
- binding nicotinamide-adenine-dinucleotide: G101 (= G155), G103 (= G157), R104 (= R158), I105 (≠ V159), Y123 (≠ W177), D124 (≠ S178), P125 (≠ Q179), I126 (≠ N180), H155 (= H209), T156 (≠ L210), P157 (≠ V211), T162 (= T216), C183 (≠ T237), A184 (≠ S238), R185 (= R239), H232 (= H287), G234 (= G289)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
35% identity, 61% coverage: 112:302/313 of query aligns to 92:284/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (= R158), Y160 (≠ W177), D161 (≠ S178), P162 (≠ Q179), I164 (≠ L181), L179 (≠ K196), T193 (≠ L210), P194 (≠ V211), S198 (≠ R215), L202 (≠ I219)
6rj6A Crystal structure of phgdh in complex with bi-4924 (see paper)
35% identity, 61% coverage: 112:302/313 of query aligns to 6:198/204 of 6rj6A
- binding 2-[4-[(1~{S})-1-[[4,5-bis(chloranyl)-1,6-dimethyl-indol-2-yl]carbonylamino]-2-oxidanyl-ethyl]phenyl]sulfonylethanoic acid: G52 (= G155), R55 (= R158), I56 (≠ V159), Y74 (≠ W177), D75 (≠ S178), P76 (≠ Q179), I77 (≠ N180), I78 (≠ L181), H106 (= H209), T107 (≠ L210), P108 (≠ V211), T113 (= T216)
7va1A Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with gdd-04-35
35% identity, 58% coverage: 112:294/313 of query aligns to 5:189/193 of 7va1A
- binding 4-[(3-ethanoylphenyl)sulfamoyl]-~{N}-[4-(3-fluorophenyl)-1,3-thiazol-2-yl]benzamide: L50 (= L154), G53 (= G157), R57 (≠ S161), Y73 (≠ W177), D74 (≠ S178), P75 (≠ Q179), I76 (≠ N180), I77 (≠ L181), T106 (≠ L210), P107 (≠ V211), L115 (≠ I219)
5ofwA Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with 3-chloro-4-fluorobenzamide (see paper)
35% identity, 58% coverage: 112:294/313 of query aligns to 7:191/195 of 5ofwA
Sites not aligning to the query:
5ofvA Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with 5-fluoro-2-methylbenzoic acid (see paper)
35% identity, 58% coverage: 112:294/313 of query aligns to 7:191/195 of 5ofvA
Sites not aligning to the query:
5ofmA Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with 5-amino-1-methyl-1h-indole
35% identity, 58% coverage: 112:294/313 of query aligns to 7:191/195 of 5ofmA