SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_091377042.1 NCBI__GCF_900105165.1:WP_091377042.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
37% identity, 96% coverage: 1:240/250 of query aligns to 2:241/253 of 6z5uK

query
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6z5uK

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binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ F11), S37 (= S36), G38 (= G37), G40 (= G39), K41 (= K40), T42 (= T41), T43 (= T42), Q86 (= Q85), E164 (= E163), H197 (= H196)
binding magnesium ion: Q86 (= Q85), E164 (= E163)

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
37% identity, 96% coverage: 1:240/250 of query aligns to 4:243/263 of 7d0aB

query
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7d0aB

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binding adenosine-5'-diphosphate: S39 (= S36), T41 (≠ S38), G42 (= G39), K43 (= K40), E140 (= E137), S142 (= S139), M145 (= M142)

7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
37% identity, 96% coverage: 1:240/250 of query aligns to 4:243/263 of 7d08B

query
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7d08B

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binding adenosine-5'-triphosphate: R14 (≠ F11), R17 (≠ N14), G40 (= G37), T41 (≠ S38), K43 (= K40), T44 (= T41), T45 (= T42), E166 (= E163)

Q9AT00 Protein TRIGALACTOSYLDIACYLGLYCEROL 3, chloroplastic; ABC transporter I family member 13; ABC transporter ABCI.13; AtABCI13; Non-intrinsic ABC protein 11; AtNAP11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 96% coverage: 1:240/250 of query aligns to 84:338/345 of Q9AT00

query
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Q9AT00

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F94 (= F11) mutation to A: Reduced ATPase activity and transport properties.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
40% identity, 93% coverage: 1:232/250 of query aligns to 1:230/240 of 4ymuJ

query
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4ymuJ

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E

E

binding adenosine-5'-triphosphate: F11 (= F11), V16 (= V16), S36 (= S36), G37 (= G37), S38 (= S38), G39 (= G39), K40 (= K40), S41 (≠ T41), T42 (= T42), E162 (= E163), H194 (= H196)
binding magnesium ion: S41 (≠ T41), E162 (= E163)

7chaI Cryo-em structure of p.Aeruginosa mlafebd with amppnp (see paper)
35% identity, 94% coverage: 2:237/250 of query aligns to 4:239/262 of 7chaI

query
sites
7chaI

I

V

E

E

I

L

K

K

D

G

I

L

Y

T

K

F

S

K

F
x
R

G

G

D

S

N

R

E

A

V

I

L

F

K

D

G

N

I

I

S

D

A

V

L

R

F

I

K

P

P

R

G

G

K

K

N

V

N

T

L

G

I

I

I

M

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

T
|
T

L

L

L

L

K

R

C

L

I

I

V

A

G

S

L

Q

H

L

E

R

P

P

T

S

K

K

G

G

Q

E

V

V

F

W

F

V

D

N

S

G

E

Q

N

N

F

L

T

P

E

Q

M

L

D

S

F

R

E

G

Q

D

R

L

V

F

P

D

I

M

R

R

T

K

Q

Q

I

F

G

G

M

V

L

L

F

F

Q

Q

N

S

S

G

A

A

L

L

F

F

D

T

S

D

M

L

T

D

V

V

E

F

E

E

N

N

I

V

M

A

F

F

P

P

L

L

N

R

L

V

F

H

T

T

N

Q

K

L

T

P

K

E

E

E

E

M

K

I

L

R

E

D

R

I

A

V

N

L

F

M

C

K

L

L

E

Q

R

A

V

V

N

G

L

L

K

R

G

G

T

A

N

V

K

E

L

L

F

M

P

P

S

D

E

E

L

L

S

S

G

G

G

G

M

M

K

K

K

R

R

R

V

V

G

A

I

L

A

A

R

R

A

A

I

I

S

A

M

L

Q

D

P

P

K

Q

Y

I

L

L

F

L

V

Y

D

D

E

E

P

P

N

F

S

V

G

G

L

Q

D

D

P

P

K

I

T

A

S

M

I

G

L

V

I

L

D

V

E

R

L

L

I

I

N

R

E

L

L

L

T

N

E

D

E

A

Y

L

Q

G

I

I

T

T

T

S

V

I

I

V

V

V

T

S

H

H

D

D

M

L

N

A

S

E

V

T

M

A

G

S

I

I

G

A

D

D

H

Y

I

I

I

Y

F

I

L

V

H

G

Q

D

G

G

K

R

K

V

W

L

W

G

E

H

G

G

S

T

N

P

H

D

E

V

I

L

A

K

H

E

T

T

D

D

N

D

K

P

E

R

L

I

N

R

D

Q

F

F

V

V

binding phosphoaminophosphonic acid-adenylate ester: R13 (≠ F11), S38 (= S36), G39 (= G37), C40 (≠ S38), G41 (= G39), K42 (= K40), T43 (= T41), T44 (= T42)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
37% identity, 90% coverage: 1:225/250 of query aligns to 2:223/241 of 4u00A

query
sites
4u00A

M

I

I

I

E

R

I

I

K

R

D

N

I

L

Y

H

K

K

S

W

F
|
F

G

G

D

P

N

L

E

H

V
|
V

L

L

K

K

G

G

I

I

S

H

A

L

L

E

F

V

K

A

P

P

G

G

K

E

N

K

N

L

L

V

I

I

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

K

R

C

T

I

I

V

N

G

R

L

L

H

E

E

D

P

F

T

Q

K

E

G

G

Q

E

V

V

F

V

F

V

D

D

S

-

E

-

N

G

F

L

T

S

E

V

M

K

D

D

F

D

E

R

Q

A

R

L

V

R

P

E

I

I

R

R

T

R

Q

E

I

V

G

G

M

M

L

V

F

F

Q

Q

N

Q

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

E

N

N

I

V

M

T

F

L

P

A

L

P

N

M

L

R

F

V

T

R

N

R

K

W

T

P

K

R

E

E

E

K

K

A

L

E

E

K

R

K

A

A

N

L

F

E

C

L

L

L

E

E

R

R

V

V

N

G

L

I

K

L

G

D

T

Q

N

A

K

R

L

K

F

Y

P

P

S

A

E

Q

L

L

S

S

G

G

G

G

M

Q

K

Q

K

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

I

L

S

A

M

M

Q

E

P

P

K

K

Y

I

L

M

F

L

V

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

-

S

-

I

-

L

M

I

V

D

G

E

E

L

V

I

L

N

D

E

V

L

M

T

R

E

D

E

L

Y

A

Q

Q

-

G

-

G

I

M

T

T

T

M

V

V

I

V

V

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

G

E

I

V

G

A

D

D

H

R

I

V

I

V

F

F

L

M

H

D

Q

G

G

G

K

Q

K

I

W

V

W

E

E

E

G

G

S

R

N

P

H

E

E

E

I

I

binding adenosine-5'-diphosphate: F12 (= F11), V17 (= V16), S37 (= S36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42)

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
36% identity, 95% coverage: 1:237/250 of query aligns to 4:239/375 of 2d62A

query
sites
2d62A

M

M

I

A

E

E

I

V

K

K

-

L

-

I

D

N

I

I

Y

W

K

K

S

R

F

F

G

G

D

D

N

V

E

T

V

A

L

V

K

K

G

D

I

L

S

S

A

L

L

E

F

I

K

K

P

D

G

G

K

E

N

F

N

L

L

V

I

L

I

L

G

G

G

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

T
|
T

L

T

L

L

K

R

C

M

I

I

V

A

G

G

L

L

H

E

E

E

P

P

T

T

K

R

G

G

Q

Q

V

I

F

Y

F

I

D

E

S

D

E

N

N

L

F

V

T

A

E

D

M

P

D

E

F

K

E

G

Q

V

R

F

V

V

P

P

I

P

R

K

T

E

Q

R

-

D

I

V

G

A

M

M

L

V

F

F

Q

Q

N

S

S

Y

A

A

L

L

F

Y

D

P

S

H

M

M

T

T

V

V

E

Y

E

D

N

N

I

I

M

A

F

F

P

P

L

L

N

K

L

L

F

-

T

R

N

K

K

V

T

P

K

K

E

Q

E

E

K

I

L

D

E

K

R

R

A

V

N

R

F

E

C

V

L

A

E

E

R

M

V

L

N

G

L

L

K

T

G

E

T

L

N

L

K

N

L

R

F

K

P

P

S

R

E

E

L

L

S

S

G

G

G

G

M

Q

K

R

K

Q

R

R

V

V

G

A

I

L

A

G

R

R

A

A

I

I

S

I

M

R

Q

R

P

P

K

K

Y

V

L

F

F

L

V

M

D

D

E

E

P

P

N

L

S

S

G

N

L

L

D

D

P

A

K

K

T

L

S

R

I

V

L

K

I

M

D

R

E

A

L

E

I

L

N

K

E

K

L

L

T

Q

E

R

E

Q

Y

L

Q

G

I

V

T

T

T

T

V

I

I

Y

V

V

T

T

H

H

D

D

M

Q

N

V

S

E

V

A

M

M

G

T

I

M

G

G

D

D

H

R

I

I

I

A

F

V

L

M

H

N

Q

K

G

G

K

E

K

L

W

Q

W

Q

E

V

G

G

S

T

N

P

H

D

E

E

I

V

A

Y

H

Y

T

-

D

-

N

-

K

K

E

P

L

V

N

N

D

T

F

F

V

V

binding pyrophosphate 2-: G42 (= G37), C43 (≠ S38), G44 (= G39), K45 (= K40), T46 (= T41), T47 (= T42)

3c4jA Abc protein artp in complex with atp-gamma-s
36% identity, 90% coverage: 1:225/250 of query aligns to 3:225/242 of 3c4jA

query
sites
3c4jA

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

Y

K

K

K

S

S

F
|
F

G

G

D

S

N

L

E

E

V
|
V

L

L

K

K

G

G

I

I

S

N

A

V

L

H

F

I

K

R

P

E

G

G

K

E

N

V

N

V

L

V

I

V

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

F

L

L

K

R

C

C

I

L

V

N

G

L

L

L

H

E

E

D

P

F

T

D

K

E

G

G

Q

E

V

I

F

I

F

I

D

D

S

G

E

I

N

N

F

L

T

K

E

A

M

K

D

D

F

T

E

N

Q

L

R

N

V

-

P

K

I

V

R

R

T

E

Q

E

I

V

G

G

M

M

L

V

F

F

Q

Q

N

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

I

I

M

T

F

L

P

A

L

P

N

M

L

K

F

V

T

R

N

K

K

W

T

P

K

R

E

E

E

K

K

A

L

E

E

A

R

K

A

A

N

M

F

E

C

L

L

L

E

D

R

K

V

V

N

G

L

L

K

K

G

D

T

K

N

A

K

H

L

A

F

Y

P

P

S

D

E

S

L

L

S

S

G

G

G

G

M

Q

K

A

K

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

I

L

S

A

M

M

Q

E

P

P

K

K

Y

I

L

M

F

L

V

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

N

K

E

Q

L

L

T

A

E

N

E

E

Y

-

Q

G

I

M

T

T

T

M

V

V

I

V

V

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

G

E

I

V

G

G

D

D

H

R

I

V

I

L

F

F

L

M

H

D

Q

G

G

G

K

Y

K

I

W

I

W

E

E

E

G

G

S

K

N

P

H

E

E

D

I

L

binding phosphothiophosphoric acid-adenylate ester: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (= T42)

3c41J Abc protein artp in complex with amp-pnp/mg2+
36% identity, 90% coverage: 1:225/250 of query aligns to 3:225/242 of 3c41J

query
sites
3c41J

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

Y

K

K

K

S

S

F
|
F

G

G

D

S

N

L

E

E

V
|
V

L

L

K

K

G

G

I

I

S

N

A

V

L

H

F

I

K

R

P

E

G

G

K

E

N

V

N

V

L

V

I

V

I

I

G

G

G

P

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

F

L

L

K

R

C

C

I

L

V

N

G

L

L

L

H

E

E

D

P

F

T

D

K

E

G

G

Q

E

V

I

F

I

F

I

D

D

S

G

E

I

N

N

F

L

T

K

E

A

M

K

D

D

F

T

E

N

Q

L

R

N

V

-

P

K

I

V

R

R

T

E

Q

E

I

V

G

G

M

M

L

V

F

F

Q

Q

N

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

I

I

M

T

F

L

P

A

L

P

N

M

L

K

F

V

T

R

N

K

K

W

T

P

K

R

E

E

E

K

K

A

L

E

E

A

R

K

A

A

N

M

F

E

C

L

L

L

E

D

R

K

V

V

N

G

L

L

K

K

G

D

T

K

N

A

K

H

L

A

F

Y

P

P

S

D

E

S

L

L

S

S

G

G

G

G

M

Q

K

A

K

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

I

L

S

A

M

M

Q

E

P

P

K

K

Y

I

L

M

F

L

V

F

D
|
D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

N

K

E

Q

L

L

T

A

E

N

E

E

Y

-

Q

G

I

M

T

T

T

M

V

V

I

V

V

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

G

E

I

V

G

G

D

D

H

R

I

V

I

L

F

F

L

M

H

D

Q

G

G

G

K

Y

K

I

W

I

W

E

E

E

G

G

S

K

N

P

H

E

E

D

I

L

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (= T42)
binding magnesium ion: S43 (≠ T41), D163 (= D162)

2olkA Abc protein artp in complex with adp-beta-s
36% identity, 90% coverage: 1:225/250 of query aligns to 3:225/242 of 2olkA

query
sites
2olkA

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

Y

K

K

K

S

S

F
|
F

G

G

D

S

N

L

E

E

V
|
V

L

L

K

K

G

G

I

I

S

N

A

V

L

H

F

I

K

R

P

E

G

G

K

E

N

V

N

V

L

V

I

V

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

F

L

L

K

R

C

C

I

L

V

N

G

L

L

L

H

E

E

D

P

F

T

D

K

E

G

G

Q

E

V

I

F

I

F

I

D

D

S

G

E

I

N

N

F

L

T

K

E

A

M

K

D

D

F

T

E

N

Q

L

R

N

V

-

P

K

I

V

R

R

T

E

Q

E

I

V

G

G

M

M

L

V

F

F

Q

Q

N

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

I

I

M

T

F

L

P

A

L

P

N

M

L

K

F

V

T

R

N

K

K

W

T

P

K

R

E

E

E

K

K

A

L

E

E

A

R

K

A

A

N

M

F

E

C

L

L

L

E

D

R

K

V

V

N

G

L

L

K

K

G

D

T

K

N

A

K

H

L

A

F

Y

P

P

S

D

E

S

L

L

S

S

G

G

G

G

M

Q

K

A

K

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

I

L

S

A

M

M

Q

E

P

P

K

K

Y

I

L

M

F

L

V

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

N

K

E

Q

L

L

T

A

E

N

E

E

Y

-

Q

G

I

M

T

T

T

M

V

V

I

V

V

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

G

E

I

V

G

G

D

D

H

R

I

V

I

L

F

F

L

M

H

D

Q

G

G

G

K

Y

K

I

W

I

W

E

E

E

G

G

S

K

N

P

H

E

E

D

I

L

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (= T42)

2oljA Abc protein artp in complex with adp/mg2+
36% identity, 90% coverage: 1:225/250 of query aligns to 3:225/242 of 2oljA

query
sites
2oljA

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

Y

K

K

K

S

S

F
|
F

G

G

D

S

N

L

E

E

V
|
V

L

L

K

K

G

G

I

I

S

N

A

V

L

H

F

I

K

R

P

E

G

G

K

E

N

V

N

V

L

V

I

V

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

F

L

L

K

R

C

C

I

L

V

N

G

L

L

L

H

E

E

D

P

F

T

D

K

E

G

G

Q

E

V

I

F

I

F

I

D

D

S

G

E

I

N

N

F

L

T

K

E

A

M

K

D

D

F

T

E

N

Q

L

R

N

V

-

P

K

I

V

R

R

T

E

Q

E

I

V

G

G

M

M

L

V

F

F

Q

Q

N

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

I

I

M

T

F

L

P

A

L

P

N

M

L

K

F

V

T

R

N

K

K

W

T

P

K

R

E

E

E

K

K

A

L

E

E

A

R

K

A

A

N

M

F

E

C

L

L

L

E

D

R

K

V

V

N

G

L

L

K

K

G

D

T

K

N

A

K

H

L

A

F

Y

P

P

S

D

E

S

L

L

S

S

G

G

G

G

M

Q

K

A

K

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

I

L

S

A

M

M

Q

E

P

P

K

K

Y

I

L

M

F

L

V

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

N

K

E

Q

L

L

T

A

E

N

E

E

Y

-

Q

G

I

M

T

T

T

M

V

V

I

V

V

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

G

E

I

V

G

G

D

D

H

R

I

V

I

L

F

F

L

M

H

D

Q

G

G

G

K

Y

K

I

W

I

W

E

E

E

G

G

S

K

N

P

H

E

E

D

I

L

binding adenosine-5'-diphosphate: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (= T42)

7ch6C Cryo-em structure of e.Coli mlafeb with amppnp (see paper)
36% identity, 95% coverage: 1:237/250 of query aligns to 4:240/265 of 7ch6C

query
sites
7ch6C

M

L

I

V

E

D

I

M

K

R

D

D

I

V

Y

S

K

F

S

T

F
x
R

G

G

D

N

N
x
R

E

C

V

I

L

F

K

D

G

N

I

I

S

S

A

L

L

T

F

V

K

P

P

R

G

G

K

K

N

I

N

T

L

A

I

I

I

M

G

G

G

P

S

S

G
|
G

S

I

G
|
G

K
|
K

T
|
T

T

T

L

L

L

L

K

R

C

L

I

I

V

G

G

G

L

Q

H

I

E

A

P

P

T

D

K

H

G

G

Q

E

V

I

F

L

F

F

D

D

S

G

E

E

N

N

F

I

T

P

E

A

M

M

D

S

F

R

E

S

Q

R

R

L

V

Y

P

T

I

V

R

R

T

K

Q

R

I

M

G

S

M

M

L

L

F

F

Q

Q

N

S

S

G

A

A

L

L

F

F

D

T

S

D

M

M

T

N

V

V

E

F

E

D

N

N

I

V

M

A

F

Y

P

P

L

L

N

R

L

E

F

H

T

T

N

Q

K

L

T

P

K

A

E

P

E

L

K

L

L

H

E

S

R

T

A

V

N

M

F

M

C

K

L

L

E

E

R

A

V

V

N

G

L

L

K

R

G

G

T

A

N

A

K

K

L

L

F

M

P

P

S

S

E

E

L

L

S

S

G

G

G

G

M

M

K

A

K

R

R

R

V

A

G

A

I

L

A

A

R

R

A

A

I

I

S

A

M

L

Q

E

P

P

K

D

Y

L

L

I

F

M

V

F

D

D

E
|
E

P

P

N

F

S

V

G

G

L

Q

D

D

P

P

K

I

T

T

S

M

I

G

L

V

I

L

D

V

E

K

L

L

I

I

N

S

E

E

L

L

T

N

E

S

E

A

Y

L

Q

G

I

V

T

T

T

C

V

V

I

V

V

V

T

S

H

H

D

D

M

V

N

P

S

E

V

V

M

L

G

S

I

I

G

A

D

D

H

H

I

A

I

W

F

I

L

L

H

A

Q

D

G

K

K

K

K

I

W

V

W

A

E

H

G

G

S

S

N

A

H

Q

E

A

I

L

A

Q

H

A

T

N

D

P

N

D

K

P

E

R

L

V

N

R

D

Q

F

F

V

L

binding phosphoaminophosphonic acid-adenylate ester: R14 (≠ F11), R17 (≠ N14), G40 (= G37), G42 (= G39), K43 (= K40), T44 (= T41), E166 (= E163)

6xgyA Crystal structure of e. Coli mlafb abc transport subunits in the dimeric state (see paper)
36% identity, 95% coverage: 1:237/250 of query aligns to 4:240/264 of 6xgyA

query
sites
6xgyA

M

L

I

V

E

D

I

M

K

R

D

D

I

V

Y

S

K

F

S

T

F
x
R

G

G

D

N

N

R

E

C

V

I

L

F

K

D

G

N

I

I

S

S

A

L

L

T

F

V

K

P

P

R

G

G

K

K

N

I

N

T

L

A

I

I

I

M

G

G

G

P

S

S

G
|
G

S

I

G
|
G

K
|
K

T
|
T

T
|
T

L

L

L

L

K

R

C

L

I

I

V

G

G

G

L

Q

H

I

E

A

P

P

T

D

K

H

G

G

Q

E

V

I

F

L

F

F

D

D

S

G

E

E

N

N

F

I

T

P

E

A

M

M

D

S

F

R

E

S

Q

R

R

L

V

Y

P

T

I

V

R

R

T

K

Q

R

I

M

G

S

M

M

L

L

F

F

Q

Q

N

S

S

G

A

A

L

L

F

F

D

T

S

D

M

M

T

N

V

V

E

F

E

D

N

N

I

V

M

A

F

Y

P

P

L

L

N

R

L

E

F

H

T

T

N

Q

K

L

T

P

K

A

E

P

E

L

K

L

L

H

E

S

R

T

A

V

N

M

F

M

C

K

L

L

E

E

R

A

V

V

N

G

L

L

K

R

G

G

T

A

N

A

K

K

L

L

F

M

P

P

S

S

E

E

L

L

S

S

G

G

G

G

M

M

K

A

K

R

R

R

V

A

G

A

I

L

A

A

R

R

A

A

I

I

S

A

M

L

Q

E

P

P

K

D

Y

L

L

I

F

M

V

F

D
|
D

E
|
E

P

P

N

F

S

V

G

G

L

Q

D

D

P

P

K

I

T

T

S

M

I

G

L

V

I

L

D

V

E

K

L

L

I

I

N

S

E

E

L

L

T

N

E

S

E

A

Y

L

Q

G

I

V

T

T

T

C

V

V

I

V

V

V

T

S

H

H

D

D

M

V

N

P

S

E

V

V

M

L

G

S

I

I

G

A

D

D

H

H

I

A

I

W

F

I

L

L

H

A

Q

D

G

K

K

K

K

I

W

V

W

A

E

H

G

G

S

S

N

A

H

Q

E

A

I

L

A

Q

H

A

T

N

D

P

N

D

K

P

E

R

L

V

N

R

D

Q

F

F

V

L

binding adenosine-5'-diphosphate: R14 (≠ F11), G40 (= G37), G42 (= G39), K43 (= K40), T44 (= T41), T45 (= T42)
binding magnesium ion: D165 (= D162), E166 (= E163)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 90% coverage: 1:225/250 of query aligns to 1:228/343 of P30750

query
sites
P30750

M

M

I

I

E

K

I

L

K

S

D

N

I

I

Y

T

K

K

S

V

F

F

G

H

D

Q

N

G

-

T

-

R

-

T

-

I

E

Q

V

A

L

L

K

N

G

N

I

V

S

S

A

L

L

H

F

V

K

P

P

A

G

G

K

Q

N

I

N

Y

L

G

I

V

I

I

G

G

G

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

K

R

C

C

I

V

V

N

G

L

L

L

H

E

E

R

P

P

T

T

K

E

G

G

Q

S

V

V

F

L

F

V

D

D

S

G

E

Q

N

E

F

L

T

T

E

T

M

L

D

S

F

E

E

S

Q

E

R

L

V

T

P

K

I

A

R

R

T

R

Q

Q

I

I

G

G

M

M

L

I

F

F

Q

Q

N

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

T

T

V

V

E

F

E

G

N

N

I

V

M

A

F

L

P

P

L

L

N

E

L

L

F

-

T

D

N

N

K

T

T

P

K

K

E

D

E

E

K

V

L

K

E

R

R

R

A

V

N

T

F

E

C

L

L

L

E

S

R

L

V

V

N

G

L

L

K

G

G

D

T

K

N

H

K

D

L

S

F

Y

P

P

S

S

E

N

L

L

S

S

G

G

G

G

M

Q

K

K

K

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

I

L

S

A

M

S

Q

N

P

P

K

K

Y

V

L

L

F

L

V

C

D

D

E
|
E

P

A

N

T

S

S

G

A

L

L

D

D

P

P

K

A

T

T

S

T

I

R

L

S

I

I

D

L

E

E

L

L

I

L

N

K

E

D

L

I

T

N

E

R

E

R

Y

L

Q

G

I

L

T

T

T

I

V

L

I

L

V

I

T

T

H

H

D

E

M

M

N

D

S

V

V

V

M

K

G

R

I

I

G

C

D

D

H

C

I

V

I

A

F

V

L

I

H

S

Q

N

G

G

K

E

K

L

W

I

W

E

E

Q

G

D

S

T

N

V

H

S

E

E

I

V

40:46 (vs. 36:42, 71% identical) binding ATP
E166 (= E163) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
34% identity, 99% coverage: 1:247/250 of query aligns to 4:233/353 of 1vciA

query
sites
1vciA

M

M

I

V

E

E

I

V

K

K

-

L

-

E

D

N

I

L

Y

T

K

K

S

R

F
|
F

G

G

D

N

N
x
F

E

T

V

A

L

V

K

N

G

K

I

L

S

N

A

L

L

T

F

I

K

K

P

D

G

G

K

E

N

F

N

L

L

V

I

L

I

L

G

G

G

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
|
T

T
|
T

L

T

L

L

K

R

C

M

I

I

V

A

G

G

L

L

H

E

E

E

P

P

T

T

K

E

G

G

Q

R

V

I

F

Y

F

F

D

G

S

D

E

R

N

D

F

V

T

T

E

Y

M

L

D

P

F

P

E

K

Q

D

R

R

V

-

P

-

I

-

R

-

T

-

Q

N

I

I

G

S

M

M

L

V

F

F

Q

Q

N

H

S

-

A

-

L

-

F

-

D

-

S

-

M

M

T

T

V

V

E

Y

E

E

N

N

I

I

M

A

F

F

P

P

L

L

N

K

L

K

F

F

T

P

N

-

K

-

T

-

K

K

E

D

E

E

K

I

L

D

E

K

R

R

A

V

N

R

F

W

C

A

L

A

E

E

R

L

V

L

N

Q

L

I

K

E

G

E

T

L

N

L

K

N

L

R

F

Y

P

P

S

A

E

Q

L

L

S

S

G

G

G

G

M

Q

K

R

K

Q

R

R

V

V

G

A

I

V

A

A

R

R

A

A

I

I

S

V

M

V

Q

E

P

P

K

D

Y

V

L

L

F

L

V

M

D

D

E

E

P

P

N

L

S

S

G

N

L

L

D

D

P

A

K

K

T

L

S

R

I

V

L

A

I

M

D

R

E

A

L

E

I

I

N

K

E

K

L

L

T

Q

E

Q

E

K

Y

L

Q

K

I

V

T

T

T

T

V

I

I

Y

V

V

T

T

H

H

D

D

M

Q

N

V

S

E

V

A

M

M

G

T

I

M

G

G

D

D

H

R

I

I

I

A

F

V

L

M

H

N

Q

R

G

G

K

Q

K

L

W

L

W

Q

E

I

G

G

S

S

N

P

H

T

E

E

I

V

A

Y

H

L

T

R

D

P

N

N

K

S

E

-

L

-

N

-

D

-

F

-

V

V

F

F

A

V

S

A

K

T

F

F

M

I

R

G

A

A

A

P

K

E

binding adenosine-5'-triphosphate: F16 (= F11), F19 (≠ N14), S41 (= S36), G42 (= G37), G44 (= G39), K45 (= K40), T46 (= T41), T47 (= T42)

7ch8I Cryo-em structure of p.Aeruginosa mlafebd with adp-v (see paper)
34% identity, 94% coverage: 2:237/250 of query aligns to 4:236/259 of 7ch8I

query
sites
7ch8I

I

V

E

E

I

L

K

K

D

G

I

L

Y

T

K

F

S

K

F
x
R

G

G

D

S

N
x
R

E

A

V

I

L

F

K

D

G

N

I

I

S

D

A

V

L

R

F

I

K

P

P

R

G

G

K

K

N

V

N

T

L

G

I

I

I

M

G

G

G

-

S
|
S

G

G

S

C

G
|
G

K
|
K

T
|
T

T
|
T

L

L

L

L

K

R

C

L

I

I

V

A

G

S

L

Q

H

L

E

R

P

P

T

S

K

K

G

G

Q

E

V

V

F

W

F

V

D

N

S

G

E

Q

N

N

F

L

T

P

E

Q

M

L

D

S

F

R

E

G

Q

D

R

L

V

F

P

D

I

M

R

R

T

K

Q

Q

I

F

G

G

M

V

L

L

F

F

Q

Q

N

S

S

G

A

A

L

L

F

F

D

T

S

D

M

L

T

D

V

V

E

F

E

E

N

N

I

V

M

A

F

F

P

P

L

L

N

R

L

V

F

H

T

T

N

Q

K

L

T

P

K

E

E

E

E

M

K

I

L

R

E

D

R

I

A

V

N

L

F

M

C

K

L

L

E

Q

R

A

V

V

N

G

L

L

K

R

G

G

T

A

N

V

K

E

L

L

F

M

P

P

S

D

E

E

L

L

S

S

G

G

G

G

M

M

K

K

K

R

R

R

V

V

G

A

I

L

A

A

R

R

A

A

I

I

S

A

M

L

Q

D

P

P

K

Q

Y

I

L

L

F

L

V

Y

D

D

E

E

P

-

N

F

S

V

G

G

L

Q

D

P

P

I

K

A

T

M

S

G

I

V

L

L

I

V

D

-

E

R

L

L

I

I

N

R

E

L

L

L

T

N

E

D

E

A

Y

L

Q

G

I

I

T

T

T

S

V

I

I

V

V

V

T

S

H

H

D

D

M

L

N

A

S

E

V

T

M

A

G

S

I

I

G

A

D

D

H

Y

I

I

I

Y

F

I

L

V

H

G

Q

D

G

G

K

R

K

V

W

L

W

G

E

H

G

G

S

T

N

P

H

D

E

V

I

L

A

K

H

E

T

T

D

D

N

D

K

P

E

R

L

I

N

R

D

Q

F

F

V

V

binding adp metavanadate: R13 (≠ F11), R16 (≠ N14), S37 (= S36), G40 (= G39), K41 (= K40), T42 (= T41), T43 (= T42)

7cgnB The overall structure of the mlafedb complex in atp-bound eqtall conformation (mutation of e170q on mlaf) (see paper)
35% identity, 95% coverage: 1:237/250 of query aligns to 4:240/263 of 7cgnB

query
sites
7cgnB

M

L

I

V

E

D

I

M

K

R

D

D

I

V

Y

S

K

F

S

T

F
x
R

G

G

D

N

N

R

E

C

V

I

L

F

K

D

G

N

I

I

S

S

A

L

L

T

F

V

K

P

P

R

G

G

K

K

N

I

N

T

L

A

I

I

I

M

G

G

G

P

S

S

G
|
G

S
x
I

G

G

K
|
K

T
|
T

T
|
T

L

L

L

L

K

R

C

L

I

I

V

G

G

G

L

Q

H

I

E

A

P

P

T

D

K

H

G

G

Q

E

V

I

F

L

F

F

D

D

S

G

E

E

N

N

F

I

T

P

E

A

M

M

D

S

F

R

E

S

Q

R

R

L

V

Y

P

T

I

V

R

R

T

K

Q

R

I

M

G

S

M

M

L

L

F

F

Q

Q

N

S

S

G

A

A

L

L

F

F

D

T

S

D

M

M

T

N

V

V

E

F

E

D

N

N

I

V

M

A

F

Y

P

P

L

L

N

R

L

E

F

H

T

T

N

Q

K

L

T

P

K

A

E

P

E

L

K

L

L

H

E

S

R

T

A

V

N

M

F

M

C

K

L

L

E

E

R

A

V

V

N

G

L

L

K

R

G

G

T

A

N

A

K

K

L

L

F

M

P

P

S

S

E

E

L

L

S

S

G

G

G

G

M

M

K

A

K

R

R

R

V

A

G

A

I

L

A

A

R

R

A

A

I

I

S

A

M

L

Q

E

P

P

K

D

Y

L

L

I

F

M

V

F

D

D

E

Q

P

P

N

F

S

V

G

G

L

Q

D

D

P

P

K

I

T

T

S

M

I

G

L

V

I

L

D

V

E

K

L

L

I

I

N

S

E

E

L

L

T

N

E

S

E

A

Y

L

Q

G

I

V

T

T

T

C

V

V

I

V

V

V

T

S

H

H

D

D

M

V

N

P

S

E

V

V

M

L

G

S

I

I

G

A

D

D

H

H

I

A

I

W

F

I

L

L

H

A

Q

D

G

K

K

K

K

I

W

V

W

A

E

H

G

G

S

S

N

A

H

Q

E

A

I

L

A

Q

H

A

T

N

D

P

N

D

K

P

E

R

L

V

N

R

D

Q

F

F

V

L

binding adenosine-5'-triphosphate: R14 (≠ F11), G40 (= G37), I41 (≠ S38), K43 (= K40), T44 (= T41), T45 (= T42)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
35% identity, 90% coverage: 1:225/250 of query aligns to 2:229/344 of 6cvlD

query
sites
6cvlD

M

M

I

I

E

K

I

L

K

S

D

N

I

I

Y

T

K

K

S

V

F
|
F

G

H

D
x
Q

N

G

-

T

-

R

-

T

-
x
I

E

Q

V

A

L

L

K

N

G

N

I

V

S

S

A

L

L

H

F

V

K

P

P

A

G

G

K

Q

N

I

N

Y

L

G

I

V

I

I

G

G

G

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

K

R

C

C

I

V

V

N

G

L

L

L

H

E

E

R

P

P

T

T

K

E

G

G

Q

S

V

V

F

L

F

V

D

D

S

G

E

Q

N

E

F

L

T

T

E

T

M

L

D

S

F

E

E

S

Q

E

R

L

V

T

P

K

I

A

R

R

T

R

Q

Q

I

I

G

G

M

M

L

I

F

F

Q

Q

N

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

T

T

V

V

E

F

E

G

N

N

I

V

M

A

F

L

P

P

L

L

N

E

L

L

F

-

T

D

N

N

K

T

T

P

K

K

E

D

E

E

K

V

L

K

E

R

R

R

A

V

N

T

F

E

C

L

L

L

E

S

R

L

V

V

N

G

L

L

K

G

G

D

T

K

N

H

K

D

L

S

F

Y

P

P

S

S

E
x
N

L

L

S
|
S

G

G

G

G

M
x
Q

K

K

K

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

I

L

S

A

M

S

Q

N

P

P

K

K

Y

V

L

L

F

L

V

C

D

D

E

Q

P

A

N

T

S

S

G

A

L

L

D

D

P

P

K

A

T

T

S

T

I

R

L

S

I

I

D

L

E

E

L

L

I

L

N

K

E

D

L

I

T

N

E

R

E

R

Y

L

Q

G

I

L

T

T

T

I

V

L

I

L

V

I

T

T

H
|
H

D

E

M

M

N

D

S

V

V

V

M

K

G

R

I

I

G

C

D

D

H

C

I

V

I

A

F

V

L

I

H

S

Q

N

G

G

K

E

K

L

W

I

W

E

E

Q

G

D

S

T

N

V

H

S

E

E

I

V

binding phosphothiophosphoric acid-adenylate ester: F12 (= F11), Q14 (≠ D13), I19 (vs. gap), S41 (= S36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (≠ T41), T47 (= T42), N141 (≠ E137), S143 (= S139),